MD13G1143700.v1.1
NAC Family

Inactive serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015983
Physical Location & Seq
Reverse (-)
11173268 .. 11180416
7149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1143700.v1.1.491

Sequence Viewer

Length: 4998 bp
ATGGCGCAGCAAATGTGCTTCGACTGCCTCCAACGCCGGATTCACTGCGACTTCTCCGGCAAACTCGTTTTCTCGTACGGCCTCTCCGATTCCGCCTTCCCTTTCGGCTCCTCCGCCGTCGTCCAGTTGTGTAATTCAAGTGGGGAAGCTGCTTCGGCTCCTCAGTTTTTGCTCAAATTTCTACCCAGTCATCCAAACGATTGCTTGACTAAATATGTTAATGAATATGTCCTGGACAATGCTGAAGGCTGCAGTGATAATGGGATTGATGGTAGGATCGCTTCAGTTGGAGTAACTCGGGATGAAGAAGAAATTAGTTCTGATGTTTACAGTAATGAAAAACCAAAGTCAGATTCTTTAATGAATGAGCCTAAGTGTTTGTCAAATGGTGGCACAGAAATATTGCTACATAGTTCTACCTGTAAGCATTCCACTAGGTTTTCTTGCTCGAGGGTTATCAGTGCATTGGTACCGGTTAGTTATGTTGGCAATTTCTCAGACTCAGTTTTTGAAGAGCTTGCTGTTAACTCTTTGACCGGGTCTTTGGAAGATCACATATTGAACTCGCTTAGTCTCTTGATTGAAGGGAAAGCTTCAGGGAGAGACAGTGTAAATTTTCTTAACTTACTTGGGATACCGTCGTTTGATGAGAATCAGTTCCCTGGCTCCCTAAGGCATCCAAATATTGCTCCCGTACTTATGATGGTTAAAGCATCCCATTATATAGATGTAGCTCTTCCAAAAACTCCGTACACATTGGAAAACATTCTCCATTACAGTCCCGAAGCCTTAAAATCTGACTGGCATATAAGGTTTCTGATATACCAGTTGCTCTCGGCTCTAGCTTATATTCATGGTTTAGGGGTTGCCCATGGCAATATATGTCCATCCAGTGTAATGCTTACTGAATCATGCTGGTCTTGGCTGTCCATTTTTGATAAGCCCGGGGTAGGATTTAGTTCAAGTTCAAGAGGCAAGGGATGCACAAGTACCTTACCAGAAAAGGTAGGTTGCTCAGAAGCTGGTTGTCCCTCTCAAGGTCTTTATGCTGATTTGAAGCGTTCCCCATCTATAGACTGGCATCGTGACTTCAACCAATGGTGGAGAGGAGAGATCAGTAATTTTGAGTATCTTCTCATCTTGAATAGATTAGCTGGGCGAAGGTGGGGTGACCACACATTTCATACAGTGATGCCTTGGGTAATAGATTTTAGCACGAAACCTGATGAGAATTCAGATGCAGGGTGGCGGGATTTAAAAAAGAGTAAATGGAGGTTGGCTAAAGGTGATGAACAGTTGGACTTCACCTATTCAACATCGGAGTTCCCACATCACGTATCTGATGAATGTCTTTCTGAATTGGCTGTCTGCAGTTATAAAGCTAGGAGGTTACCTTTGAGTGTTCTACGTATGGCTGTTCGCTCAGTCTATGAACCTAATGAATATCCCTCTACCATGCAAAGACTTTATCAGTGGACCCCTGATGAGTGTATTCCGGAGTTTTACTGCAATCCTCAAATTTTTCATTCACTTCATGCTGGTATGACTGACTTGGCGGTACCTTCATGGGCAGATGGTCCTGAGGAATTCATTAAATTGCATCGTGATGCTTTAGAAAGCGAACGGGTCTCACGTGAACTCCATCACTGGATTGATATCACCTTTGGCTACAAGATGTCAGGTGAGGCAGCTGTTTCTGCAAAGAATGTAATGCTTCCTTCATCAGAACCTATGATGCCGAGGTCTGCAGGACGCCGTCAGCTGTTCACTCAACCTCACCCTATGCGTCGAGGTGCAGTGCGGAAACCATGTGATAGCACCAATGAATCAACTTTGCATCATGAATTAAGGAGTGAGAGTTCTGTCCTATCTGAAACTGCTTACTTGCAAGAATTAGAAGATGCATCTTCCTTTTGTGAACATGCTATGCATTTAAGTCCCCTCTATGGCTACCATCTAGACTTTGTGAGGGACATTTCTCCTGTACAAGAGTCATCAGGTGAGAATGTTAACAAAAGTATACCTCTGTCATCTGACAGTAAGAAAAACCAGTTGTCACGGTTACACATTGATACAAGTTATCTACTTGAGCATATTGAAGTGGAGGATGAAGGTTCCCGGGGATATCAAGAGCTATTTCTTTGGAGGGAAAAATCATCTTGTTTAAAGATGTTCTCTGAAGAAATTGCAAGGGATATATTTTCTGTCGGTTGTCTCTTAGCAGAACTTCATTTGAGGAAGCCACTTTTTGACCCAAGCTCATTGACCATGTACTTGGACAGTGGTGTATTACCTGGATTGATGCATGAACTTCCTCCTCACACCAAATTACTTGTTGAAGCTTGCATTCAAAAGGATTGCATGAGGAGACCATCGGCTAAGTGTCTGTTGGAATCACCATATTTTCCTACTACGGTTAAGGCGTCCTACTTATTTCTGGCCCCTCTCCAGCTGCTGGCTAAAGGTGGATCCTGCCTTCAGTATGCTGCAAATTTTGCAAAGCAAGGAGTCCTCAAGGCAATGGGAACATTTGCTGCGGAAATGTGTGCTCCTTATTGCTTATCATTTGTGGTGACTCCCTTATCAGATACTGAAGCCGAATGGGCTTATATACTACTGAAAGAATTCATCAAAAGTTTGACCCCTAAAGCAGTGAAGACATTAGTCTTGCCTGCCATCCAGAAGATTTTACAGACTACAGTTTATTCACATCTAAAGGTTTCTATTCTACAAGACTCCTTTGTTCGAGAGATATGGAATCGAACTGGCAAACAAGTATATCTTGAAACTGTACATCCTCTTGTTATATTGAACTTATATACTGCCGCTCATAATAGTTCAGCAACCGCTGCTTCCGTGCTGCTTATTTGCTCAAGTGAAGAACTTGGTATACCTATTACCATTCATCAGACAATCTTGCCCCTGATTCAATGCTTTGGGAAAGGTCTCAGTAGTGATGGAATTGACGTGCTGGTTCGAATTGGTGGTCTATTGGGAGAGAACTTCATTGTCAGACAGATGCTGCCGTTGCTAAAACATGTGTTTCATTCCTGTATAGGCGTTTCACGTATGAATAAGCCTGAGCCTGTCCACAGCTGGAGTGCTTTTGCTCTTATTGATTGTTTGACGACAATAGATGGCCTTGTTGCATTTTTGCCAAGGGAGGTGGTTGCGAAGGAGCTAGTTGAAGATAAAAGTTGCCCACAGGTTCTGGTTCTTATGGAGACAAATTTTGAACATAGAGTGCTTCAGGTTGCTGCTACTACCCTAATGGCAGTTTGTGAACGGATAGGACCAGATTTGACAGCATTGCATGTTTTGCCACAACTTAAAGAGCTATTTGATGAGCTTGCTTTCTCACCGAGAAAAGCTAATGCTTCTACTTCCTTTGGCAGAAGGCTGAAGAGTTCAAAGCCAATAATTGACGGAGGGGGTCTGATTGAAAGTCGTATGGACCTTGCGTTGCTTCTGTATCCTTCTTTTGCATACCTTCTTGGGATAGAGAAGCTTCGTCAGTGTTGTACAACATGGTTATTACTTGAGCAGTATCTTCTACAATATCATAACTGGAAGTGGGAACACACAGGAGAATTGTCTCGAAGTGGTTCAGACACTATACTCAGTAAAAGATCTGCATTCAGCAATCGCTCAACTCCTGAATACAGTCCTGCTAAGCTGTTGCTTAATGGGGTTGGATGGTCAATTCCACAATCACAAGGAACTAGAGGTGCCAAAAACATGATGCCTCAAAAACGGTTCTCTGAAATGCATCAGAGTTCAGCTGAAATGCATCCATCAAATTTTAAATTCGAGCCCTGGTTTTGGTTCCCTAGTCCAGCTTCTAGCTGGGATGGGCCTGATTTTCTGGCCCGTGCTGGGGGTGTGAAGGATGAACATCCATGGAAGATCAGAGCATCTGTTATATACTCAGTTCGTGCGCACCCTGGGGCCTTAAGGTATTTAGCTGTCTGCCCAGATGAATGTACGGTTTTTACAGCAGGAATTGGTGCAGGGTTCAAAGGGACTGTTCAAAAATGGGAGCTGGCTAGAATCAATTGTGTTTCGGGATACTATGGCCACGAGGAGGTTGTGAATGATATTTGTGTCTTATCGTCTAGTGGAAGAGTTGCATCCTGTGATGGAACGATACATGTTTGGAGTAGCCGAACTGGAAAATTAATTGCAGTATACTCTGAACCATCTTTGGATTCTGCACATGTTGCAAGCCCTCCATCTTCTTCCTCCAGGGCCAATCTTGACCAGGTTAATATGCTCAGTTCCAATGCACAGTCAAGTGGAATATTGACTGGGGCATTTGATGGGAGCTTGTATACTTGTATGCATCAGACAGAACTTGGTGAAAAGCTTGTAGTTGGCACTGGAAATGGTTCACTCAGGTTCATTGATGTTGTCCGAGGACAAAAGCTTCACCTTTGGAGGGGGGATTCTATGGAGTCTGGATATCCTTCTCTTATTTCTTCCATATGCTCCTGTGGGTCTGATAAAATGCAGCCGGATGGAGCTTCTTCACCCTCTTGGATTGCAGCTGGATTGAGTTCTGGTCATTGTAGATTATTTGATGCAAGGAGCGGAAATGTTATATCCTCTTGGAAGGCTCATGATGGATATGTGACAAAGTTGGCTGCACCAGAGGACCATTTACTTGTGTCCAGCTCTCTTGACAGGTCTTTAAGAATTTGGGACTTGAGAAGGAATTGGCCATCTCAGCCCACAATTCTTCAGGGTCACACAGACGGTGTATCTTCATTCTCCGTGTGGGGCCAAGACGTTATTTCAATTTCCAGAAATAAGATTGGAATTTCTTCTCTGTCTAAATCCTCCGATGATGACGGACAGCAGGCTGTTACATGTCAAAAACTGTACATGGCAGATCACGGAGCAAGAAATTTTTCGGTGTTGTCAAGCATAAGTATTTTACCCTTCTCAAGGTTGTTCCTGGTTGGAACTGAAGATGGCTACCTAAGAATATGTTGCTGA

Protein Analysis

1666

Amino Acids

184.71

Weight (kDa)

6.21

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beach PF02138 365 - 575 3.4e-41 Beige/BEACH domain
Beta-prop_WDR36-Utp21_1st PF25171 1341 - 1593 1.5e-08 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 1454 - 1572 4.5e-06 Prp19 WD40 domain
WD40_Prp19 PF24814 1510 - 1596 2.7e-06 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 1511 - 1601 1.8e-09 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 1511 - 1591 1.6e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 1521 - 1599 3.1e-07 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 1537 - 1603 9.6e-06 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015983)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18525
fragaria_vesca FvH4_4g21250 FvH4_4g21250
malus_domestica MD13G1143700.v1.1
prunus_persica Prupe.1G108200_v2.0.a1
pyrus_communis pycom13g12460
rosa_chinensis RchiOBHm_Chr4g0426871
rosa_laevigata RLG00000007272
rosa_multiflora Rmu_sc0000953.1_g000015
rosa_roxburghii Rroxscaffold_5G00368840
rosa_samantha Rh4AG270500 Rh4BG276800 Rh4CG291500 Rh4DG274000
rosa_wichuraiana Rw4G023470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1377
Acc16I TGCGCA 1 cut(s) 3939
Acc65I GGTACC 2 cut(s) 469, 1558
AccB1I GGYRCC 3 cut(s) 469, 1558, 3728
AccB7I CCANNNNNTGG 1 cut(s) 2455
AccBSI CCGCTC 2 cut(s) 2828, 4590
AccI GTMKAC 4 cut(s) 2020, 2890, 4188, 4331
AccIII TCCGGA 1 cut(s) 1495
AciI CCGC 9 cut(s) 93, 114, 1249, 1556, 1801, 2537, 2826, 2847, 4590
AclWI GGATC 3 cut(s) 284, 2463, 2476
AcoI YGGCCR 2 cut(s) 4075, 4718
AcvI CACGTG 1 cut(s) 1634
AcyI GRCGYC 2 cut(s) 1753, 2423
AflII CTTAAG 1 cut(s) 3952
AflIII ACRYGT 4 cut(s) 3037, 4150, 4216, 4868
AgeI ACCGGT 1 cut(s) 472
AjiI CACGTC 1 cut(s) 2968
AjnI CCWGG 8 cut(s) 231, 661, 2293, 3815, 3943, 4244, 4260, 4956
AjuI GAANNNNNNNTTGG 4 cut(s) 24, 56, 337, 369
AloI GAACNNNNNNTCC 2 cut(s) 1843, 1875
Alw21I GWGCWC 1 cut(s) 2551
Alw26I GTCTC 8 cut(s) 578, 597, 1633, 2219, 2362, 2951, 3218, 3600
AlwI GGATC 3 cut(s) 284, 2463, 2476
AlwNI CAGNNNCTG 5 cut(s) 1022, 2455, 2591, 3022, 3211
Ama87I CYCGRG 4 cut(s) 297, 448, 944, 2118
Aor13HI TCCGGA 1 cut(s) 1495
ArsI GACNNNNNNTTYG 6 cut(s) 491, 523, 526, 558, 2971, 3003
AseI ATTAAT 1 cut(s) 4178
AsiGI ACCGGT 1 cut(s) 472
Asp700I GAANNNNTTC 7 cut(s) 656, 765, 2624, 3604, 4387, 4822, 4909
Asp718I GGTACC 2 cut(s) 469, 1558
AspLEI GCGC 2 cut(s) 7, 3940
AsuC2I CCSGG 5 cut(s) 538, 945, 946, 2119, 2120
AsuII TTCGAA 1 cut(s) 2977
AvaI CYCGRG 4 cut(s) 297, 448, 944, 2118
AvaII GGWCC 5 cut(s) 1476, 1577, 3293, 3454, 4654
AxyI CCTNAGG 2 cut(s) 671, 1581
BaeI ACNNNNGTAYC 2 cut(s) 626, 659
BalI TGGCCA 2 cut(s) 4077, 4720
BamHI GGATCC 1 cut(s) 2468
BanI GGYRCC 3 cut(s) 469, 1558, 3728
BanII GRGCYC 1 cut(s) 3816
BarI GAAGNNNNNNTAC 2 cut(s) 265, 297
BauI CACGAG 1 cut(s) 4079
BbrPI CACGTG 1 cut(s) 1634
BbsI GAAGAC 1 cut(s) 2663
Bbv12I GWGCWC 1 cut(s) 2551
BceAI ACGGC 4 cut(s) 94, 101, 1740, 3010
BciT130I CCWGG 8 cut(s) 233, 663, 2295, 3817, 3945, 4246, 4262, 4958
BciVI GTATCC 3 cut(s) 627, 3483, 4061
BcnI CCSGG 5 cut(s) 538, 945, 946, 2119, 2120
BcoDI GTCTC 8 cut(s) 578, 597, 1633, 2219, 2362, 2951, 3218, 3600
BfmI CTRYAG 5 cut(s) 250, 1071, 1369, 1746, 2697
BfrI CTTAAG 1 cut(s) 3952
BfuI GTATCC 3 cut(s) 627, 3483, 4061
BglI GCCNNNNNGGC 1 cut(s) 2603
BglII AGATCT 1 cut(s) 3629
BlpI GCTNAGC 1 cut(s) 3672
Bme18I GGWCC 5 cut(s) 1476, 1577, 3293, 3454, 4654
BmeT110I CYCGRG 4 cut(s) 297, 448, 944, 2118
BmgBI CACGTC 1 cut(s) 2968
BmrI ACTGGG 2 cut(s) 180, 4317
BmuI ACTGGG 2 cut(s) 180, 4317
BoxI GACNNNNGTC 1 cut(s) 2663
BpiI GAAGAC 1 cut(s) 2663
BpmI CTGGAG 3 cut(s) 2432, 3118, 4228
Bpu10I CCTNAGC 1 cut(s) 3081
Bpu1102I GCTNAGC 1 cut(s) 3672
Bpu14I TTCGAA 1 cut(s) 2977
BpuEI CTTGAG 7 cut(s) 1020, 2108, 2498, 2857, 3560, 4726, 4930
BpuMI CCSGG 5 cut(s) 538, 945, 946, 2119, 2120
BsaAI YACGTR 4 cut(s) 1336, 1409, 1634, 3068
BsaBI GATNNNNATC 3 cut(s) 651, 1655, 3894
BsaHI GRCGYC 2 cut(s) 1753, 2423
BsaI GGTCTC 3 cut(s) 1633, 2362, 2951
BsaWI WCCGGW 2 cut(s) 472, 1495
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 592, 622, 1623, 1653, 1843, 1873
Bse118I RCCGGY 1 cut(s) 472
Bse21I CCTNAGG 2 cut(s) 671, 1581
Bse3DI GCAATG 2 cut(s) 2526, 3308
Bse8I GATNNNNATC 3 cut(s) 651, 1655, 3894
BseAI TCCGGA 1 cut(s) 1495
BseBI CCWGG 8 cut(s) 233, 663, 2295, 3817, 3945, 4246, 4262, 4958
BseJI GATNNNNATC 3 cut(s) 651, 1655, 3894
BseMI GCAATG 2 cut(s) 2526, 3308
BseRI GAGGAG 6 cut(s) 100, 150, 1122, 2307, 2380, 4097
BseYI CCCAGC 3 cut(s) 1154, 3846, 3875
BsgI GTGCAG 4 cut(s) 1815, 4029, 4197, 4629
BshNI GGYRCC 3 cut(s) 469, 1558, 3728
BshTI ACCGGT 1 cut(s) 472
BsiHKAI GWGCWC 1 cut(s) 2551
BsiHKCI CYCGRG 4 cut(s) 297, 448, 944, 2118
BsiSI CCGG 8 cut(s) 37, 57, 473, 537, 945, 1496, 2119, 4514
BsiWI CGTACG 1 cut(s) 75
BslFI GGGAC 6 cut(s) 765, 1014, 1923, 1985, 4036, 4715
BsmAI GTCTC 8 cut(s) 578, 597, 1633, 2219, 2362, 2951, 3218, 3600
BsmFI GGGAC 6 cut(s) 765, 1014, 1923, 1985, 4036, 4715
BsmI GAATGC 3 cut(s) 427, 2346, 3635
Bso31I GGTCTC 3 cut(s) 1633, 2362, 2951
BsoBI CYCGRG 4 cut(s) 297, 448, 944, 2118
Bsp119I TTCGAA 1 cut(s) 2977
Bsp1286I GDGCHC 2 cut(s) 2551, 3816
Bsp13I TCCGGA 1 cut(s) 1495
Bsp1407I TGTACA 4 cut(s) 1984, 2791, 3521, 4881
Bsp143I GATC 7 cut(s) 276, 550, 1113, 2468, 3629, 3906, 4891
Bsp1720I GCTNAGC 1 cut(s) 3672
Bsp19I CCATGG 2 cut(s) 871, 3899
BspACI CCGC 9 cut(s) 93, 114, 1249, 1556, 1801, 2537, 2826, 2847, 4590
BspEI TCCGGA 1 cut(s) 1495
BspHI TCATGA 2 cut(s) 1840, 4618
BspMAI CTGCAG 3 cut(s) 254, 1373, 1750
BspPI GGATC 3 cut(s) 284, 2463, 2476
BspQI GCTCTTC 2 cut(s) 507, 741
BspT104I TTCGAA 1 cut(s) 2977
BspT107I GGYRCC 3 cut(s) 469, 1558, 3728
BspTI CTTAAG 1 cut(s) 3952
BspTNI GGTCTC 3 cut(s) 1633, 2362, 2951
BsrBI CCGCTC 2 cut(s) 2828, 4590
BsrDI GCAATG 2 cut(s) 2526, 3308
BsrFI RCCGGY 1 cut(s) 472
BsrGI TGTACA 4 cut(s) 1984, 2791, 3521, 4881
BssAI RCCGGY 1 cut(s) 472
BssMI GATC 7 cut(s) 276, 550, 1113, 2468, 3629, 3906, 4891
BssNAI GTATAC 4 cut(s) 2021, 2891, 4189, 4332
BssNI GRCGYC 2 cut(s) 1753, 2423
BssSI CACGAG 1 cut(s) 4079
BssT1I CCWWGG 4 cut(s) 871, 1196, 3158, 3899
Bst1107I GTATAC 4 cut(s) 2021, 2891, 4189, 4332
Bst2BI CACGAG 1 cut(s) 4079
Bst2UI CCWGG 8 cut(s) 233, 663, 2295, 3817, 3945, 4246, 4262, 4958
Bst6I CTCTTC 4 cut(s) 507, 741, 3398, 4117
BstACI GRCGYC 2 cut(s) 1753, 2423
BstAFI CTTAAG 1 cut(s) 3952
BstAPI GCANNNNNTGC 5 cut(s) 981, 2495, 2849, 3319, 4220
BstAUI TGTACA 4 cut(s) 1984, 2791, 3521, 4881
BstBAI YACGTR 4 cut(s) 1336, 1409, 1634, 3068
BstBI TTCGAA 1 cut(s) 2977
BstC8I GCNNGC 8 cut(s) 519, 2344, 2457, 2673, 3351, 4044, 4225, 4860
BstDSI CCRYGG 2 cut(s) 871, 3899
BstEII GGTNACC 2 cut(s) 1169, 1389
BstENI CCTNNNNNAGG 1 cut(s) 4945
BstHHI GCGC 2 cut(s) 7, 3940
BstKTI GATC 7 cut(s) 279, 553, 1116, 2471, 3632, 3909, 4894
BstMAI GTCTC 8 cut(s) 578, 597, 1633, 2219, 2362, 2951, 3218, 3600
BstMBI GATC 7 cut(s) 276, 550, 1113, 2468, 3629, 3906, 4891
BstNI CCWGG 8 cut(s) 233, 663, 2295, 3817, 3945, 4246, 4262, 4958
BstNSI RCATGY 6 cut(s) 1925, 3041, 3317, 4154, 4220, 4872
BstPAI GACNNNNGTC 1 cut(s) 2663
BstPI GGTNACC 2 cut(s) 1169, 1389
BstSFI CTRYAG 5 cut(s) 250, 1071, 1369, 1746, 2697
BstSNI TACGTA 1 cut(s) 1409
BstV2I GAAGAC 1 cut(s) 2663
BstX2I RGATCY 2 cut(s) 2468, 3629
BstXI CCANNNNNNTGG 1 cut(s) 2275
BstYI RGATCY 2 cut(s) 2468, 3629
BstZ17I GTATAC 4 cut(s) 2021, 2891, 4189, 4332
Bsu36I CCTNAGG 2 cut(s) 671, 1581
BsuI GTATCC 3 cut(s) 627, 3483, 4061
BtgI CCRYGG 2 cut(s) 871, 3899
BtrI CACGTC 1 cut(s) 2968
BtsI GCAGTG 4 cut(s) 43, 259, 1803, 2658
Cac8I GCNNGC 8 cut(s) 519, 2344, 2457, 2673, 3351, 4044, 4225, 4860
CaiI CAGNNNCTG 5 cut(s) 1022, 2455, 2591, 3022, 3211
CciI TCATGA 2 cut(s) 1840, 4618
CfoI GCGC 2 cut(s) 7, 3940
Cfr10I RCCGGY 1 cut(s) 472
Cfr9I CCCGGG 2 cut(s) 944, 2118
CseI GACGC 3 cut(s) 1761, 1775, 2412
CsiI ACCWGGT 1 cut(s) 4260
CspAI ACCGGT 1 cut(s) 472
CspCI CAANNNNNGTGG 2 cut(s) 3147, 3182
DpnI GATC 7 cut(s) 278, 552, 1115, 2470, 3631, 3908, 4893
DpnII GATC 7 cut(s) 276, 550, 1113, 2468, 3629, 3906, 4891
DraI TTTAAA 3 cut(s) 1257, 2166, 3805
EaeI YGGCCR 2 cut(s) 4075, 4718
Eam1104I CTCTTC 4 cut(s) 507, 741, 3398, 4117
EarI CTCTTC 4 cut(s) 507, 741, 3398, 4117
EciI GGCGGA 2 cut(s) 82, 103
Eco105I TACGTA 1 cut(s) 1409
Eco130I CCWWGG 4 cut(s) 871, 1196, 3158, 3899
Eco24I GRGCYC 1 cut(s) 3816
Eco31I GGTCTC 3 cut(s) 1633, 2362, 2951
Eco32I GATATC 3 cut(s) 1657, 2126, 4463
Eco47I GGWCC 5 cut(s) 1476, 1577, 3293, 3454, 4654
Eco72I CACGTG 1 cut(s) 1634
Eco81I CCTNAGG 2 cut(s) 671, 1581
Eco88I CYCGRG 4 cut(s) 297, 448, 944, 2118
Eco91I GGTNACC 2 cut(s) 1169, 1389
EcoNI CCTNNNNNAGG 1 cut(s) 4945
EcoO109I RGGNCCY 1 cut(s) 3948
EcoO65I GGTNACC 2 cut(s) 1169, 1389
EcoRI GAATTC 3 cut(s) 1231, 1586, 2624
EcoRII CCWGG 8 cut(s) 231, 661, 2293, 3815, 3943, 4244, 4260, 4956
EcoRV GATATC 3 cut(s) 1657, 2126, 4463
EcoT14I CCWWGG 4 cut(s) 871, 1196, 3158, 3899
EcoT22I ATGCAT 6 cut(s) 1906, 1932, 2307, 3771, 3792, 4344
EcoT38I GRGCYC 1 cut(s) 3816
ErhI CCWWGG 4 cut(s) 871, 1196, 3158, 3899
FalI AAGNNNNNCTT 2 cut(s) 2766, 2798
FaqI GGGAC 6 cut(s) 765, 1014, 1923, 1985, 4036, 4715
FauI CCCGC 1 cut(s) 1242
FauNDI CATATG 1 cut(s) 4484
FblI GTMKAC 4 cut(s) 2020, 2890, 4188, 4331
FriOI GRGCYC 1 cut(s) 3816
FspAI RTGCGCAY 1 cut(s) 3939
FspI TGCGCA 1 cut(s) 3939
GlaI GCGC 2 cut(s) 6, 3939
GsaI CCCAGC 3 cut(s) 1158, 3850, 3879
GsuI CTGGAG 3 cut(s) 2432, 3118, 4228
HapII CCGG 8 cut(s) 37, 57, 473, 537, 945, 1496, 2119, 4514
HgaI GACGC 3 cut(s) 1761, 1775, 2412
HhaI GCGC 2 cut(s) 7, 3940
Hin1I GRCGYC 2 cut(s) 1753, 2423
Hin6I GCGC 2 cut(s) 5, 3938
HinP1I GCGC 2 cut(s) 5, 3938
HincII GTYRAC 2 cut(s) 526, 2011
HindII GTYRAC 2 cut(s) 526, 2011
HindIII AAGCTT 5 cut(s) 591, 2340, 3506, 4364, 4424
HpaI GTTAAC 2 cut(s) 526, 2011
HpaII CCGG 8 cut(s) 37, 57, 473, 537, 945, 1496, 2119, 4514
Hpy99I CGWCG 3 cut(s) 122, 643, 1791
HpyCH4IV ACGT 6 cut(s) 1335, 1408, 1633, 2967, 3067, 4788
HpySE526I ACGT 6 cut(s) 1335, 1408, 1633, 2967, 3067, 4788
Hsp92I GRCGYC 2 cut(s) 1753, 2423
HspAI GCGC 2 cut(s) 5, 3938
Kpn2I TCCGGA 1 cut(s) 1495
KpnI GGTACC 2 cut(s) 473, 1562
KspAI GTTAAC 2 cut(s) 526, 2011
Kzo9I GATC 7 cut(s) 276, 550, 1113, 2468, 3629, 3906, 4891
LguI GCTCTTC 2 cut(s) 507, 741
MabI ACCWGGT 1 cut(s) 4260
MaeII ACGT 6 cut(s) 1335, 1408, 1633, 2967, 3067, 4788
MalI GATC 7 cut(s) 278, 552, 1115, 2470, 3631, 3908, 4893
MbiI CCGCTC 2 cut(s) 2828, 4590
MboI GATC 7 cut(s) 276, 550, 1113, 2468, 3629, 3906, 4891
MfeI CAATTG 1 cut(s) 4054
MflI RGATCY 2 cut(s) 2468, 3629
MhlI GDGCHC 2 cut(s) 2551, 3816
MlsI TGGCCA 2 cut(s) 4077, 4720
MluNI TGGCCA 2 cut(s) 4077, 4720
MlyI GAGTC 6 cut(s) 494, 2000, 2517, 2569, 2729, 4463
MmeI TCCRAC 6 cut(s) 55, 268, 1278, 2370, 3673, 4942
Mox20I TGGCCA 2 cut(s) 4077, 4720
Mph1103I ATGCAT 6 cut(s) 1906, 1932, 2307, 3771, 3792, 4344
MroI TCCGGA 1 cut(s) 1495
MroXI GAANNNNTTC 7 cut(s) 656, 765, 2624, 3604, 4387, 4822, 4909
MscI TGGCCA 2 cut(s) 4077, 4720
MslI CAYNNNNRTG 1 cut(s) 1605
Msp20I TGGCCA 2 cut(s) 4077, 4720
MspA1I CMGCKG 7 cut(s) 1691, 1762, 2452, 2849, 3096, 3782, 4547
MspCI CTTAAG 1 cut(s) 3952
MspI CCGG 8 cut(s) 37, 57, 473, 537, 945, 1496, 2119, 4514
MunI CAATTG 1 cut(s) 4054
Mva1269I GAATGC 3 cut(s) 427, 2346, 3635
MvaI CCWGG 8 cut(s) 233, 663, 2295, 3817, 3945, 4246, 4262, 4958
NciI CCSGG 5 cut(s) 538, 945, 946, 2119, 2120
NcoI CCATGG 2 cut(s) 871, 3899
NdeI CATATG 1 cut(s) 4484
NdeII GATC 7 cut(s) 276, 550, 1113, 2468, 3629, 3906, 4891
NmeAIII GCCGAG 2 cut(s) 815, 1764
NmuCI GTSAC 6 cut(s) 1085, 1169, 2055, 2572, 4630, 4745
NsbI TGCGCA 1 cut(s) 3939
NsiI ATGCAT 6 cut(s) 1906, 1932, 2307, 3771, 3792, 4344
NspI RCATGY 6 cut(s) 1925, 3041, 3317, 4154, 4220, 4872
NspV TTCGAA 1 cut(s) 2977
PaeR7I CTCGAG 1 cut(s) 448
PagI TCATGA 2 cut(s) 1840, 4618
PasI CCCWGGG 1 cut(s) 3944
PciI ACATGT 4 cut(s) 3037, 4150, 4216, 4868
PciSI GCTCTTC 2 cut(s) 507, 741
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 1630, 2420
PctI GAATGC 3 cut(s) 427, 2346, 3635
PdmI GAANNNNTTC 7 cut(s) 656, 765, 2624, 3604, 4387, 4822, 4909
Pfl23II CGTACG 1 cut(s) 75
PflFI GACNNNGTC 2 cut(s) 538, 1755
PflMI CCANNNNNTGG 1 cut(s) 2455
PfoI TCCNGGA 1 cut(s) 231
PinAI ACCGGT 1 cut(s) 472
PleI GAGTC 6 cut(s) 494, 1999, 2516, 2569, 2729, 4462
PmaCI CACGTG 1 cut(s) 1634
PmlI CACGTG 1 cut(s) 1634
PpsI GAGTC 6 cut(s) 494, 1999, 2516, 2569, 2729, 4462
Ppu21I YACGTR 4 cut(s) 1336, 1409, 1634, 3068
PscI ACATGT 4 cut(s) 3037, 4150, 4216, 4868
PshAI GACNNNNGTC 1 cut(s) 2663
PshBI ATTAAT 1 cut(s) 4178
PsiI TTATAA 1 cut(s) 1377
Psp6I CCWGG 8 cut(s) 231, 661, 2293, 3815, 3943, 4244, 4260, 4956
PspCI CACGTG 1 cut(s) 1634
PspEI GGTNACC 2 cut(s) 1169, 1389
PspFI CCCAGC 3 cut(s) 1154, 3846, 3875
PspGI CCWGG 8 cut(s) 231, 661, 2293, 3815, 3943, 4244, 4260, 4956
PspLI CGTACG 1 cut(s) 75
PspXI VCTCGAGB 1 cut(s) 448
PsrI GAACNNNNNNTAC 2 cut(s) 2874, 2906
PstI CTGCAG 3 cut(s) 254, 1373, 1750
PstNI CAGNNNCTG 5 cut(s) 1022, 2455, 2591, 3022, 3211
PsuI RGATCY 2 cut(s) 2468, 3629
PsyI GACNNNGTC 2 cut(s) 538, 1755
PvuII CAGCTG 6 cut(s) 1691, 1762, 2452, 3096, 3782, 4547
RseI CAYNNNNRTG 1 cut(s) 1605
SapI GCTCTTC 2 cut(s) 507, 741
Sau3AI GATC 7 cut(s) 276, 550, 1113, 2468, 3629, 3906, 4891
SchI GAGTC 6 cut(s) 494, 2000, 2517, 2569, 2729, 4463
SduI GDGCHC 2 cut(s) 2551, 3816
SexAI ACCWGGT 1 cut(s) 4260
SfcI CTRYAG 5 cut(s) 250, 1071, 1369, 1746, 2697
Sfr274I CTCGAG 1 cut(s) 448
SfuI TTCGAA 1 cut(s) 2977
SinI GGWCC 5 cut(s) 1476, 1577, 3293, 3454, 4654
SlaI CTCGAG 1 cut(s) 448
SmaI CCCGGG 2 cut(s) 946, 2120
SmiMI CAYNNNNRTG 1 cut(s) 1605
SmlI CTYRAG 9 cut(s) 448, 1035, 2087, 2513, 2872, 3539, 3952, 4705, 4945
SmoI CTYRAG 9 cut(s) 448, 1035, 2087, 2513, 2872, 3539, 3952, 4705, 4945
SnaBI TACGTA 1 cut(s) 1409
SsiI CCGC 9 cut(s) 93, 114, 1249, 1556, 1801, 2537, 2826, 2847, 4590
SspI AATATT 3 cut(s) 402, 685, 4302
StyI CCWWGG 4 cut(s) 871, 1196, 3158, 3899
TaiI ACGT 6 cut(s) 1338, 1411, 1636, 2970, 3070, 4791
TaqI TCGA 8 cut(s) 21, 449, 1789, 2746, 2761, 2977, 3598, 3810
TatI WGTACW 5 cut(s) 1984, 2271, 2791, 3521, 4881
TauI GCSGC 1 cut(s) 2828
TseFI GTSAC 6 cut(s) 1085, 1169, 2055, 2572, 4630, 4745
Tsp45I GTSAC 6 cut(s) 1085, 1169, 2055, 2572, 4630, 4745
TspGWI ACGGA 7 cut(s) 738, 2845, 3301, 3441, 4762, 4866, 4911
TspMI CCCGGG 2 cut(s) 944, 2118
Tth111I GACNNNGTC 2 cut(s) 538, 1755
Van91I CCANNNNNTGG 1 cut(s) 2455
Vha464I CTTAAG 1 cut(s) 3952
VpaK11BI GGWCC 5 cut(s) 1476, 1577, 3293, 3454, 4654
VspI ATTAAT 1 cut(s) 4178
XagI CCTNNNNNAGG 1 cut(s) 4945
XbaI TCTAGA 1 cut(s) 1957
XceI RCATGY 6 cut(s) 1925, 3041, 3317, 4154, 4220, 4872
XcmI CCANNNNNNNNNTGG 2 cut(s) 1074, 3843
XhoI CTCGAG 1 cut(s) 448
XmaI CCCGGG 2 cut(s) 944, 2118
XmiI GTMKAC 4 cut(s) 2020, 2890, 4188, 4331
XmnI GAANNNNTTC 7 cut(s) 656, 765, 2624, 3604, 4387, 4822, 4909
Zsp2I ATGCAT 6 cut(s) 1906, 1932, 2307, 3771, 3792, 4344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.