Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0015983

Physical Location & Seq

Genomic Coordinates Forward (+)

52209777 .. 52216312

Gene Structure 6536 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ39585

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4470 bp

ATGGAAGATCACATATTGAACTCACTTGGTCTCTTGATTGAAGGGAAAGCTTCGGGAAGAGATAGTGTCAACTTTCTTAATTTACTTGGGGTACCATCATTTGATGAGAATCCGTTTCCTGGCTCCTTGAGGCATCCGAATATCGCTCCCATACTCGGGATGGTTAAGACATCCGGTTATATTGATGTAGTTCTTCCCAAAGCTCCATATACCTTGGAAAACATTCTCCATTATAGCCCTGATGCCCTAAAGTCTGATTGGCATATAAGGTTTCTAGTGTATCAGTTACTCTCAGCTCTAGCTTATATTCATGGTTTAGGGGTTGCTCATGGAAATATATGTCCTTCCAGTGTAATGCTTTCTGAGTCATGCTGGTCTTGGCTGTGTGTATGTGATAAGCCTGGGTTAGGGTTTAATTCAAGTTCAAGAGGTAATGGGAATACAATTACTGCACCTGAAAAGGTTGGTTGCTCTGTACCAGGTTGTCCTTCTCAAGGTCTTTATGCTGATTTGAAGCTTTCCCCGTCTATAGACTGGCATTGGGACTTTAATCAGTGGTGGAGGGGAGAGATTAGTAATTTTGAATATCTACTCATCTTGAATAGATTAGCTGGGAGAAGGTGGGGTGATCACACATTTCATACAGTGATGCCATGGGTAATAGATTTTAGTACAAAACCTGATGAGAATTCTGATGCAGGGTGGCGGGATTTGAGTAAGAGTAAATGGAGGTTGGCTAAAGGTGATGAGCAGTTGGACTTCACCTATTCAACCTCAGAATTCCCACATCATGTTTCTGATGAATGTCTTTCTGAATTGGCTGTCTGCAGCTATAAAGCAAGGAGGTTGCCTTTGAGTGTTCTACGTATGGCTGTTCGCGCAGTCTATGAACCTAATGAATATCCTTCTACAATGCAAAGACTCTATCAATGGACCCCTGATGAATGCATTCCGGAGTTTTACTGTGATCCCCATGTTTTCAATTCACTTCATGCTGGTATGACCGACTTAGCTGTACCTTCATGGGCAGGTGGTCCTGAGGAATTCATCAAATTGCATCGTGAAGCATTAGAAAGCGATCGGGTTTCCCGCCAACTTCATCACTGGATTGATATCACCTTTGGTTACAAGATGTCAGGTCAGGCAGCTGTTGCAGCAAAGAATGTAATGCTCCCTTCTTCAGAGTCCATGATGCCAAGGTCAGTGGGACGCCGTCAGCTTTTTACTGAACCTCATCCTATGCGTCGATGTGCAATATGGAAGCCATGTGACAGCACCAATGAGTCAGCTTCATATCAAGGAGAAATGAATGAATTAAGGAGCAAAAGTTCTGTCCTATCTGACATGGCTTATTTGCAACTATTAGAAGATGCATCAGCCTTTTGCGAACATGCTATGGAATTGAGTGCCCTCTATGGGTACCATCTCGAATTTGGCAAGGACATTGCTCCTGTAGAAGAACAATCGAGTGAGAATGTTAAGAAAAGCATACCTCGATCATCTGACACTAAGAAACACCAGTGGTTGCCATTACAAATTGATACAAATTATCTTCTTGAGCATATTAAGGTGGAGGATGAAGGCTCCACTGGATATCAAGAGCTATTACTTTGGAAGCAGAAATCATCTTGTTTGAAGACATTTACTGAAGATGTTGCAAGGGACATATTTTCTATTGGTTGTCTCTTAGCAGAACTTTATTTGAGGAGGCCACTTTTTGATCCAACCTCACTGACCATGTACTTGGACAGTGGTCTCTTACCTGGACTAATGCATGAACTTCCTCCTCACACCAAATTACTAGTTGAAGCATGCATTCAAAAGGATTGCATGAGGAGGCCATCAGCGAAGTCTCTTTTGGAATCACCTTACTTTCCTTCTACAGTCAAGGCATCCTACTTGTTTCTTGCCCCACTCCATCTTCGGGCTAAAGATGGATCCTGCCTCCATTATGCTGCAAACTATGCAAAGCAAGGAGTCCTCAAGGCAATGGGAACATTTGCAGCTGAAATGTGTGCTCCTTTTTGCTTATCACTTGTGGTGATTCCTTTATCAGATATTGAAGCAGAATGGGCTTATACAATACTAAAAGAATTTATTAAAAGTTTGACACCTAAAGCAGTAAAGACAATAGTCTTGCCTGCTATCCAGAGGATTTTACAGACTACAGGTTATTCACATCTAAAGGTTTCTATTCTGCAAGACTCATTTGTACAAGAGATATGGAATCGGGTTGGCAAACAAGCATATCTCGAAACCGTACATCCTCTAGTAATAGTGAACTTGTATGCTGCTGCTCATAAGAGTTCAGCAGCTGCTGCTTCCGTGCTGCTGCTCGGCTCAAGTGAAGAGCTTGGTATTCCTATTACTATTCATCAGACAATCTTACCATTGATCCAGTGCTTTGGGAAAGGTCTAAGTACTGATGGAATTGATGTGCTCATTCGAATCGGTGGTCTATTGGGGGAAGACTTCATAGTCAGACAAATGCTACCATTGCTAAAACATGTAGTTCGTTCGTGCATTGACATTTCACGTATGAATAAGCCTGAGCCTGTCCACAGCTGGAGTGCTTTCGCTCTTATTGATTCTTTAATGACAATAGATGGCCTGGTTGCATTCTTGCCAAGGGAGGTGGTTGTAAGGGAGCTAATTGAAGATAGAAGTTGCCTGCATGTTCTGGTTCTTATGCAAAAAAGTTTGGAATATAAAGTGGTTCAGGTTGCTGCCACAACCCTTATGGTTGTTTGTCAGCGGATAGGACCAGATTTGACAGCATTACATGTTTTGCCACAACTGAAGGAGCTATTCGATGAACTTGCTTTCTCACCAGAAACTGCTAATACATCTACTTCCCCTGGCAGAAGGTTGAAGATTTCAAAGCTAAAAGACGGGGCTCTGATTGAAAGTCGTATGGACCTTGCGGTGCTTCTGTATCCTCATTTTGCATCCCTTCTTGGGATAGAGAAGCTTCGTCAGTGTTGTGCAACATGGTTATTACTTGAGCGATATCTTCTACAATTTCATAATTGGAAGTGGGAACACACAGGAGAATTGTCGCGAAGTGGTTCAGACACTGTACTCAATAAAAGAGCATACAGAAAGGGCTCAACTCCTGAATACAGTCCTGCTAAGCTGTTGCTTAATGGGGTTGGATGGTCAATTCCGCAATCTCAAGGAACTAGAGGTGCCAAAAACTTGATGCCTCAGAAACGGTTTCATGCAGTGCATCAGAATCCAACAGAAATGCATGCAGCAGCAGCAGCATCAAATTATAAATATGAGCCCTGGTTTTGGTTCCCTAGTCCAGCTGCTAGCTGGGATGGGCCTGATTTCCTTGGGCGTGCTGGGGGTGTGAAAGATGAACATCCATGGAAGATCAGAGCATCTGTTATACACTCCGTCCGTGCTCATCCTGGAGCCTTAAGATATTTAGCTGTCTGTCCAGATGAATCCACAGTTTTTACTGCAGGGATTGGTGCAGGCTTCAGAGGAACTGTTCAGAAGTGGGAGCTGAGTAGAATTAATTGTGTATCTGGATACTACGGCCATGAAGAGGTTGTGAACGATATTTGCATCTTGTCGTCTAGTAGAAGAGTTGCTTCCTGTGACGGAACAATACATGTCTGGAATAGCCGAACAGGGAAATTAATTTCAGTATTTTCTGAACCATCAGTGGATTCTGCACATATTGCAAGCCCTCCATCTTCTTCTTCAAGGGCCAGTGTTGACCAGAGTAATATTCTCAGTCCCAATACAATGTCCAGTGGAATATTGACTGGTGCATTTGATGGGAGCCTGTATACTTGTATGCATCAAACTGAATTTGGTGAAACCCTTGTTGCTGGCACTGGAGATGGTTCTCTGAGGTTCATTGATGTTGTCCGAGGGGAAAAGCTGCATCTTTGGAAGGGGAATTATCTGGAGTCAGGCTTTCCTTCTATTGTTTCTACCTTATGCTCTTGTGGGTCTGACAAAATGCAACCAGATGGAGCTTCTTCACCATCTTGGATTGCAGCTGGAATGAGTTCTGGCCATTGTAGGTTATTTGATGCAAGGAGCGGAAATGTTATTGCCTCTTGGCGGGCTCATGATGGATATGTTACAAAGTTGGCTGCACCAGAAGACTATTTGCTTGTGTCGAGCTCTCTTGACAAGACTTTAAGGATTTGGGACTTGAGGAGGAATGTGCCATCTCAGCCCACAATTCTTAGAGGTCATACAGATGGTGTATCTGCCTTCTCTGTATGGGGCCAAGATGTTATTTCAATTGCCAGAAATAAAATTGGACTGTCTTCTTTATCTAAATCCCCTGATGATGATGGACAGCAGGCCGTTACATGTCAAAGGCTCTACATGGCAGACCACGGAGCAAGAAGCTTTTCAGTGTTATCAAGCATGAGCATTTTACCCTTCTCAAGGTTGTTCCTTGTTGGGACAGAAGATGGTTACTTAAGAATATGTTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006950 GO:0006952 GO:0006955 GO:0007034 GO:0008104 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0015031 GO:0015833 GO:0031461 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042742 GO:0042886 GO:0043207 GO:0044424 GO:0044464 GO:0045087 GO:0045184 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0098542 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Beach Pkinase WD40

Protein Analysis

1489

Amino Acids

165.42

Weight (kDa)

6.56

Isoelectric Point (pI)

52.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	41 - 122	9.8e-06	Protein kinase domain
Beach	PF02138	184 - 394	1e-41	Beige/BEACH domain
Beta-prop_WDR36-Utp21_1st	PF25171	1200 - 1391	4.2e-07	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	1264 - 1413	9.2e-07	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	1277 - 1396	2.2e-08	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	1335 - 1419	4.3e-09	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	1338 - 1415	4.8e-07	WDR5 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18525
fragaria_vesca	FvH4_4g21250 FvH4_4g21250
malus_domestica	MD13G1143700.v1.1
prunus_persica	Prupe.1G108200_v2.0.a1
pyrus_communis	pycom13g12460
rosa_chinensis	RchiOBHm_Chr4g0426871
rosa_laevigata	RLG00000007272
rosa_multiflora	Rmu_sc0000953.1_g000015
rosa_roxburghii	Rroxscaffold_5G00368840
rosa_samantha	Rh4AG270500 Rh4BG276800 Rh4CG291500 Rh4DG274000
rosa_wichuraiana	Rw4G023470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3276
AarI	CACCTGC	1 cut(s)	1019
AasI	GACNNNNNNGTC	1 cut(s)	2477
Acc36I	ACCTGC	1 cut(s)	1019
Acc65I	GGTACC	2 cut(s)	91, 1419
AccB1I	GGYRCC	3 cut(s)	91, 1419, 3188
AccBSI	CCGCTC	1 cut(s)	4062
AccI	GTMKAC	1 cut(s)	3803
AccII	CGCG	2 cut(s)	879, 3061
AccIII	TCCGGA	1 cut(s)	952
AciI	CCGC	7 cut(s)	706, 1090, 2755, 2924, 3167, 4062, 4084
AclWI	GGATC	5 cut(s)	962, 1715, 1932, 1945, 2389
AcoI	YGGCCR	2 cut(s)	3547, 4033
AcsI	RAATTY	6 cut(s)	688, 779, 1043, 1430, 2093, 3824
AcuI	CTGAAG	4 cut(s)	1164, 1668, 2819, 3472
AcyI	GRCGYC	1 cut(s)	1210
AflII	CTTAAG	2 cut(s)	3424, 4453
AflIII	ACRYGT	4 cut(s)	2506, 2782, 3622, 4340
AhlI	ACTAGT	1 cut(s)	1801
AjnI	CCWGG	8 cut(s)	118, 400, 478, 1762, 2610, 2857, 3287, 3415
AjuI	GAANNNNNNNTTGG	2 cut(s)	1841, 1873
AloI	GAACNNNNNNTCC	2 cut(s)	1312, 1344
Alw21I	GWGCWC	4 cut(s)	2020, 2442, 3412, 4148
Alw26I	GTCTC	4 cut(s)	35, 1688, 1760, 1857
AlwI	GGATC	5 cut(s)	962, 1715, 1932, 1945, 2389
AlwNI	CAGNNNCTG	4 cut(s)	2315, 2318, 2837, 3077
Ama87I	CYCGRG	1 cut(s)	155
Aor13HI	TCCGGA	1 cut(s)	952
AoxI	GGCC	9 cut(s)	1709, 1838, 2608, 3326, 3547, 3720, 4033, 4252, 4332
ApoI	RAATTY	6 cut(s)	688, 779, 1043, 1430, 2093, 3824
ArsI	GACNNNNNNTTYG	4 cut(s)	1317, 1349, 2440, 2472
AseI	ATTAAT	2 cut(s)	3525, 3650
Asp700I	GAANNNNTTC	5 cut(s)	222, 948, 3067, 4027, 4381
Asp718I	GGTACC	2 cut(s)	91, 1419
AspLEI	GCGC	1 cut(s)	881
AspS9I	GGNCC	7 cut(s)	933, 1034, 2762, 2917, 3326, 3720, 4252
AsuHPI	GGTGA	9 cut(s)	638, 754, 755, 1108, 1857, 2053, 2820, 3842, 3993
AsuII	TTCGAA	1 cut(s)	2446
AsuNHI	GCTAGC	1 cut(s)	3314
AvaI	CYCGRG	1 cut(s)	155
AvaII	GGWCC	4 cut(s)	933, 1034, 2762, 2917
AxyI	CCTNAGG	1 cut(s)	1038
BaeGI	GKGCMC	1 cut(s)	1411
BalI	TGGCCA	1 cut(s)	4035
BamHI	GGATCC	1 cut(s)	1937
BanI	GGYRCC	3 cut(s)	91, 1419, 3188
BanII	GRGCYC	5 cut(s)	2899, 3110, 3288, 4090, 4148
BbsI	GAAGAC	4 cut(s)	1643, 2475, 4131, 4287
Bbv12I	GWGCWC	4 cut(s)	2020, 2442, 3412, 4148
BceAI	ACGGC	3 cut(s)	1197, 3562, 4319
BcgI	CGANNNNNNTGC	2 cut(s)	2801, 2835
BciT130I	CCWGG	8 cut(s)	120, 402, 480, 1764, 2612, 2859, 3289, 3417
BciVI	GTATCC	2 cut(s)	2946, 3533
BclI	TGATCA	1 cut(s)	628
BcoDI	GTCTC	4 cut(s)	35, 1688, 1760, 1857
BcuI	ACTAGT	1 cut(s)	1801
BfaI	CTAG	8 cut(s)	275, 299, 1802, 2270, 3183, 3303, 3315, 3588
BfmI	CTRYAG	6 cut(s)	528, 826, 1452, 1881, 2166, 3468
BfrI	CTTAAG	2 cut(s)	3424, 4453
BfuAI	ACCTGC	1 cut(s)	1019
BfuI	GTATCC	2 cut(s)	2946, 3533
BlpI	GCTNAGC	1 cut(s)	3132
BmcAI	AGTACT	1 cut(s)	2422
Bme1390I	CCNGG	8 cut(s)	120, 402, 480, 1764, 2612, 2859, 3289, 3417
Bme18I	GGWCC	4 cut(s)	933, 1034, 2762, 2917
BmeT110I	CYCGRG	1 cut(s)	155
BmgT120I	GGNCC	7 cut(s)	933, 1034, 2762, 2917, 3326, 3720, 4252
BmrFI	CCNGG	8 cut(s)	120, 402, 480, 1764, 2612, 2859, 3289, 3417
BmtI	GCTAGC	1 cut(s)	3318
BoxI	GACNNNNGTC	2 cut(s)	1752, 2132
BpiI	GAAGAC	4 cut(s)	1643, 2475, 4131, 4287
BpmI	CTGGAG	4 cut(s)	2587, 3438, 3873, 3944
Bpu10I	CCTNAGC	1 cut(s)	2550
Bpu1102I	GCTNAGC	1 cut(s)	3132
Bpu14I	TTCGAA	1 cut(s)	2446
BpuEI	CTTGAG	9 cut(s)	148, 477, 1577, 1967, 2326, 3023, 3159, 4198, 4402
BsaAI	YACGTR	2 cut(s)	866, 2537
BsaBI	GATNNNNATC	3 cut(s)	108, 1112, 3366
BsaHI	GRCGYC	1 cut(s)	1210
BsaI	GGTCTC	2 cut(s)	35, 1760
BsaWI	WCCGGW	2 cut(s)	173, 952
BsaXI	ACNNNNNCTCC	2 cut(s)	1312, 1342
Bse21I	CCTNAGG	1 cut(s)	1038
Bse3DI	GCAATG	3 cut(s)	1443, 1995, 2495
Bse8I	GATNNNNATC	3 cut(s)	108, 1112, 3366
BseAI	TCCGGA	1 cut(s)	952
BseBI	CCWGG	8 cut(s)	120, 402, 480, 1764, 2612, 2859, 3289, 3417
BseJI	GATNNNNATC	3 cut(s)	108, 1112, 3366
BseMI	GCAATG	3 cut(s)	1443, 1995, 2495
BseRI	GAGGAG	4 cut(s)	1720, 1776, 1849, 4195
BseSI	GKGCMC	1 cut(s)	1411
BseYI	CCCAGC	3 cut(s)	611, 3318, 3347
BsgI	GTGCAG	4 cut(s)	435, 3501, 3669, 4101
Bsh1236I	CGCG	2 cut(s)	879, 3061
Bsh1285I	CGRYCG	1 cut(s)	1081
BshFI	GGCC	9 cut(s)	1711, 1840, 2610, 3328, 3549, 3722, 4035, 4254, 4334
BshNI	GGYRCC	3 cut(s)	91, 1419, 3188
BsiEI	CGRYCG	1 cut(s)	1081
BsiHKAI	GWGCWC	4 cut(s)	2020, 2442, 3412, 4148
BsiHKCI	CYCGRG	1 cut(s)	155
BsiSI	CCGG	2 cut(s)	174, 953
BslFI	GGGAC	6 cut(s)	557, 1221, 1676, 3735, 4187, 4450
BsmAI	GTCTC	4 cut(s)	35, 1688, 1760, 1857
BsmFI	GGGAC	6 cut(s)	557, 1221, 1676, 3735, 4187, 4450
BsmI	GAATGC	4 cut(s)	948, 950, 1815, 2618
BsnI	GGCC	9 cut(s)	1711, 1840, 2610, 3328, 3549, 3722, 4035, 4254, 4334
Bso31I	GGTCTC	2 cut(s)	35, 1760
BsoBI	CYCGRG	1 cut(s)	155
Bsp119I	TTCGAA	1 cut(s)	2446
Bsp1286I	GDGCHC	9 cut(s)	1411, 2020, 2442, 2899, 3110, 3288, 3412, 4090, 4148
Bsp13I	TCCGGA	1 cut(s)	952
Bsp1407I	TGTACA	1 cut(s)	2212
Bsp143I	GATC	9 cut(s)	7, 628, 967, 1078, 1496, 1720, 1937, 2394, 3378
Bsp1720I	GCTNAGC	1 cut(s)	3132
Bsp19I	CCATGG	2 cut(s)	653, 3371
Bsp68I	TCGCGA	1 cut(s)	3061
BspACI	CCGC	7 cut(s)	706, 1090, 2755, 2924, 3167, 4062, 4084
BspANI	GGCC	9 cut(s)	1711, 1840, 2610, 3328, 3549, 3722, 4035, 4254, 4334
BspEI	TCCGGA	1 cut(s)	952
BspFNI	CGCG	2 cut(s)	879, 3061
BspHI	TCATGA	1 cut(s)	4090
BspMAI	CTGCAG	2 cut(s)	830, 3472
BspMI	ACCTGC	1 cut(s)	1019
BspOI	GCTAGC	1 cut(s)	3318
BspPI	GGATC	5 cut(s)	962, 1715, 1932, 1945, 2389
BspQI	GCTCTTC	1 cut(s)	2343
BspT104I	TTCGAA	1 cut(s)	2446
BspT107I	GGYRCC	3 cut(s)	91, 1419, 3188
BspTI	CTTAAG	2 cut(s)	3424, 4453
BspTNI	GGTCTC	2 cut(s)	35, 1760
BsrBI	CCGCTC	1 cut(s)	4062
BsrDI	GCAATG	3 cut(s)	1443, 1995, 2495
BsrGI	TGTACA	1 cut(s)	2212
BssMI	GATC	9 cut(s)	7, 628, 967, 1078, 1496, 1720, 1937, 2394, 3378
BssNAI	GTATAC	1 cut(s)	3804
BssNI	GRCGYC	1 cut(s)	1210
BssT1I	CCWWGG	6 cut(s)	213, 653, 1196, 2627, 3337, 3371
Bst1107I	GTATAC	1 cut(s)	3804
Bst2UI	CCWGG	8 cut(s)	120, 402, 480, 1764, 2612, 2859, 3289, 3417
Bst6I	CTCTTC	4 cut(s)	52, 2343, 3549, 3589
BstACI	GRCGYC	1 cut(s)	1210
BstAFI	CTTAAG	2 cut(s)	3424, 4453
BstAPI	GCANNNNNTGC	4 cut(s)	1151, 1964, 2318, 3692
BstAUI	TGTACA	1 cut(s)	2212
BstBAI	YACGTR	2 cut(s)	866, 2537
BstBI	TTCGAA	1 cut(s)	2446
BstDSI	CCRYGG	3 cut(s)	653, 3371, 4366
BstENI	CCTNNNNNAGG	3 cut(s)	405, 492, 4417
BstFNI	CGCG	2 cut(s)	879, 3061
BstHHI	GCGC	1 cut(s)	881
BstKTI	GATC	9 cut(s)	10, 631, 970, 1081, 1499, 1723, 1940, 2397, 3381
BstMAI	GTCTC	4 cut(s)	35, 1688, 1760, 1857
BstMBI	GATC	9 cut(s)	7, 628, 967, 1078, 1496, 1720, 1937, 2394, 3378
BstMCI	CGRYCG	1 cut(s)	1081
BstNI	CCWGG	8 cut(s)	120, 402, 480, 1764, 2612, 2859, 3289, 3417
BstNSI	RCATGY	8 cut(s)	1394, 1815, 2510, 2678, 2786, 3254, 3626, 4344
BstPAI	GACNNNNGTC	2 cut(s)	1752, 2132
BstSCI	CCNGG	8 cut(s)	118, 400, 478, 1762, 2610, 2857, 3287, 3415
BstSFI	CTRYAG	6 cut(s)	528, 826, 1452, 1881, 2166, 3468
BstSLI	GKGCMC	1 cut(s)	1411
BstSNI	TACGTA	1 cut(s)	866
BstUI	CGCG	2 cut(s)	879, 3061
BstV2I	GAAGAC	4 cut(s)	1643, 2475, 4131, 4287
BstX2I	RGATCY	1 cut(s)	1937
BstXI	CCANNNNNNTGG	2 cut(s)	1744, 2405
BstYI	RGATCY	1 cut(s)	1937
BstZ17I	GTATAC	1 cut(s)	3804
Bsu36I	CCTNAGG	1 cut(s)	1038
BsuI	GTATCC	2 cut(s)	2946, 3533
BsuRI	GGCC	9 cut(s)	1711, 1840, 2610, 3328, 3549, 3722, 4035, 4254, 4334
BtgI	CCRYGG	3 cut(s)	653, 3371, 4366
BtsI	GCAGTG	1 cut(s)	3231
BtuMI	TCGCGA	1 cut(s)	3061
BveI	ACCTGC	1 cut(s)	1019
CaiI	CAGNNNCTG	4 cut(s)	2315, 2318, 2837, 3077
CciI	TCATGA	1 cut(s)	4090
CfoI	GCGC	1 cut(s)	881
Cfr13I	GGNCC	7 cut(s)	933, 1034, 2762, 2917, 3326, 3720, 4252
CseI	GACGC	2 cut(s)	1218, 1232
CsiI	ACCWGGT	1 cut(s)	478
CspCI	CAANNNNNGTGG	4 cut(s)	1185, 1220, 2616, 2651
DpnI	GATC	9 cut(s)	9, 630, 969, 1080, 1498, 1722, 1939, 2396, 3380
DpnII	GATC	9 cut(s)	7, 628, 967, 1078, 1496, 1720, 1937, 2394, 3378
DrdI	GACNNNNNNGTC	1 cut(s)	2477
DseDI	GACNNNNNNGTC	1 cut(s)	2477
EaeI	YGGCCR	2 cut(s)	3547, 4033
Eam1104I	CTCTTC	4 cut(s)	52, 2343, 3549, 3589
EarI	CTCTTC	4 cut(s)	52, 2343, 3549, 3589
Ecl136II	GAGCTC	1 cut(s)	4146
Eco105I	TACGTA	1 cut(s)	866
Eco130I	CCWWGG	6 cut(s)	213, 653, 1196, 2627, 3337, 3371
Eco24I	GRGCYC	5 cut(s)	2899, 3110, 3288, 4090, 4148
Eco31I	GGTCTC	2 cut(s)	35, 1760
Eco32I	GATATC	3 cut(s)	1114, 1595, 3011
Eco47I	GGWCC	4 cut(s)	933, 1034, 2762, 2917
Eco53kI	GAGCTC	1 cut(s)	4146
Eco57I	CTGAAG	4 cut(s)	1164, 1668, 2819, 3472
Eco81I	CCTNAGG	1 cut(s)	1038
Eco88I	CYCGRG	1 cut(s)	155
EcoICRI	GAGCTC	1 cut(s)	4146
EcoNI	CCTNNNNNAGG	3 cut(s)	405, 492, 4417
EcoRI	GAATTC	3 cut(s)	688, 779, 1043
EcoRII	CCWGG	8 cut(s)	118, 400, 478, 1762, 2610, 2857, 3287, 3415
EcoRV	GATATC	3 cut(s)	1114, 1595, 3011
EcoT14I	CCWWGG	6 cut(s)	213, 653, 1196, 2627, 3337, 3371
EcoT22I	ATGCAT	6 cut(s)	950, 1375, 1776, 1817, 3252, 3816
EcoT38I	GRGCYC	5 cut(s)	2899, 3110, 3288, 4090, 4148
ErhI	CCWWGG	6 cut(s)	213, 653, 1196, 2627, 3337, 3371
FalI	AAGNNNNNCTT	2 cut(s)	3586, 3618
FaqI	GGGAC	6 cut(s)	557, 1221, 1676, 3735, 4187, 4450
FauI	CCCGC	3 cut(s)	699, 1097, 4077
FbaI	TGATCA	1 cut(s)	628
FblI	GTMKAC	1 cut(s)	3803
FriOI	GRGCYC	5 cut(s)	2899, 3110, 3288, 4090, 4148
FspBI	CTAG	8 cut(s)	275, 299, 1802, 2270, 3183, 3303, 3315, 3588
GlaI	GCGC	1 cut(s)	880
GsaI	CCCAGC	3 cut(s)	615, 3322, 3351
GsuI	CTGGAG	4 cut(s)	2587, 3438, 3873, 3944
HaeIII	GGCC	9 cut(s)	1711, 1840, 2610, 3328, 3549, 3722, 4035, 4254, 4334
HapII	CCGG	2 cut(s)	174, 953
HgaI	GACGC	2 cut(s)	1218, 1232
HhaI	GCGC	1 cut(s)	881
Hin1I	GRCGYC	1 cut(s)	1210
Hin6I	GCGC	1 cut(s)	879
HinP1I	GCGC	1 cut(s)	879
HincII	GTYRAC	2 cut(s)	70, 3730
HindII	GTYRAC	2 cut(s)	70, 3730
HindIII	AAGCTT	4 cut(s)	48, 515, 2969, 4378
HpaII	CCGG	2 cut(s)	174, 953
HphI	GGTGA	9 cut(s)	638, 754, 755, 1108, 1857, 2053, 2820, 3842, 3993
Hpy166II	GTNNAC	6 cut(s)	70, 2281, 2560, 3565, 3730, 3804
Hpy8I	GTNNAC	6 cut(s)	70, 2281, 2560, 3565, 3730, 3804
Hpy99I	CGWCG	1 cut(s)	1248
HpyCH4IV	ACGT	2 cut(s)	865, 2536
HpySE526I	ACGT	2 cut(s)	865, 2536
Hsp92I	GRCGYC	1 cut(s)	1210
HspAI	GCGC	1 cut(s)	879
Kpn2I	TCCGGA	1 cut(s)	952
KpnI	GGTACC	2 cut(s)	95, 1423
Ksp22I	TGATCA	1 cut(s)	628
Kzo9I	GATC	9 cut(s)	7, 628, 967, 1078, 1496, 1720, 1937, 2394, 3378
LguI	GCTCTTC	1 cut(s)	2343
MabI	ACCWGGT	1 cut(s)	478
MaeI	CTAG	8 cut(s)	275, 299, 1802, 2270, 3183, 3303, 3315, 3588
MaeII	ACGT	2 cut(s)	865, 2536
MaeIII	GTNAC	7 cut(s)	285, 1124, 1268, 3608, 4102, 4336, 4448
MalI	GATC	9 cut(s)	9, 630, 969, 1080, 1498, 1722, 1939, 2396, 3380
MbiI	CCGCTC	1 cut(s)	4062
MboI	GATC	9 cut(s)	7, 628, 967, 1078, 1496, 1720, 1937, 2394, 3378
MfeI	CAATTG	1 cut(s)	4269
MflI	RGATCY	1 cut(s)	1937
MhlI	GDGCHC	9 cut(s)	1411, 2020, 2442, 2899, 3110, 3288, 3412, 4090, 4148
MlsI	TGGCCA	1 cut(s)	4035
MluNI	TGGCCA	1 cut(s)	4035
MlyI	GAGTC	7 cut(s)	374, 915, 1193, 1292, 1986, 2198, 3935
MmeI	TCCRAC	4 cut(s)	735, 1748, 3133, 3263
Mox20I	TGGCCA	1 cut(s)	4035
Mph1103I	ATGCAT	6 cut(s)	950, 1375, 1776, 1817, 3252, 3816
MroI	TCCGGA	1 cut(s)	952
MroXI	GAANNNNTTC	5 cut(s)	222, 948, 3067, 4027, 4381
MscI	TGGCCA	1 cut(s)	4035
MslI	CAYNNNNRTG	1 cut(s)	4224
Msp20I	TGGCCA	1 cut(s)	4035
MspA1I	CMGCKG	7 cut(s)	1148, 2006, 2315, 2565, 2755, 3311, 4019
MspCI	CTTAAG	2 cut(s)	3424, 4453
MspI	CCGG	2 cut(s)	174, 953
MspR9I	CCNGG	8 cut(s)	120, 402, 480, 1764, 2612, 2859, 3289, 3417
MunI	CAATTG	1 cut(s)	4269
Mva1269I	GAATGC	4 cut(s)	948, 950, 1815, 2618
MvaI	CCWGG	8 cut(s)	120, 402, 480, 1764, 2612, 2859, 3289, 3417
MvnI	CGCG	2 cut(s)	879, 3061
NcoI	CCATGG	2 cut(s)	653, 3371
NdeII	GATC	9 cut(s)	7, 628, 967, 1078, 1496, 1720, 1937, 2394, 3378
NheI	GCTAGC	1 cut(s)	3314
NmeAIII	GCCGAG	1 cut(s)	2316
NmuCI	GTSAC	2 cut(s)	1268, 3608
NruI	TCGCGA	1 cut(s)	3061
NsiI	ATGCAT	6 cut(s)	950, 1375, 1776, 1817, 3252, 3816
NspI	RCATGY	8 cut(s)	1394, 1815, 2510, 2678, 2786, 3254, 3626, 4344
NspV	TTCGAA	1 cut(s)	2446
PaeI	GCATGC	2 cut(s)	1815, 3254
PagI	TCATGA	1 cut(s)	4090
PaqCI	CACCTGC	1 cut(s)	1019
PciI	ACATGT	4 cut(s)	2506, 2782, 3622, 4340
PciSI	GCTCTTC	1 cut(s)	2343
PctI	GAATGC	4 cut(s)	948, 950, 1815, 2618
PdmI	GAANNNNTTC	5 cut(s)	222, 948, 3067, 4027, 4381
PfeI	GAWTC	9 cut(s)	109, 1862, 2044, 2227, 2448, 2588, 3235, 3452, 3680
PflFI	GACNNNGTC	1 cut(s)	1212
PfoI	TCCNGGA	1 cut(s)	3415
Ple19I	CGATCG	1 cut(s)	1081
PleI	GAGTC	7 cut(s)	373, 915, 1192, 1291, 1985, 2198, 3934
PpsI	GAGTC	7 cut(s)	373, 915, 1192, 1291, 1985, 2198, 3934
Ppu21I	YACGTR	2 cut(s)	866, 2537
PscI	ACATGT	4 cut(s)	2506, 2782, 3622, 4340
PshAI	GACNNNNGTC	2 cut(s)	1752, 2132
PshBI	ATTAAT	2 cut(s)	3525, 3650
PsiI	TTATAA	1 cut(s)	3276
Psp124BI	GAGCTC	1 cut(s)	4148
Psp6I	CCWGG	8 cut(s)	118, 400, 478, 1762, 2610, 2857, 3287, 3415
PspFI	CCCAGC	3 cut(s)	611, 3318, 3347
PspGI	CCWGG	8 cut(s)	118, 400, 478, 1762, 2610, 2857, 3287, 3415
PspPI	GGNCC	7 cut(s)	933, 1034, 2762, 2917, 3326, 3720, 4252
PstI	CTGCAG	2 cut(s)	830, 3472
PstNI	CAGNNNCTG	4 cut(s)	2315, 2318, 2837, 3077
PsuI	RGATCY	1 cut(s)	1937
PsyI	GACNNNGTC	1 cut(s)	1212
PvuI	CGATCG	1 cut(s)	1081
PvuII	CAGCTG	6 cut(s)	1148, 2006, 2315, 2565, 3311, 4019
RruI	TCGCGA	1 cut(s)	3061
RseI	CAYNNNNRTG	1 cut(s)	4224
SacI	GAGCTC	1 cut(s)	4148
SapI	GCTCTTC	1 cut(s)	2343
Sau3AI	GATC	9 cut(s)	7, 628, 967, 1078, 1496, 1720, 1937, 2394, 3378
Sau96I	GGNCC	7 cut(s)	933, 1034, 2762, 2917, 3326, 3720, 4252
ScaI	AGTACT	1 cut(s)	2422
SchI	GAGTC	7 cut(s)	374, 915, 1193, 1292, 1986, 2198, 3935
ScrFI	CCNGG	8 cut(s)	120, 402, 480, 1764, 2612, 2859, 3289, 3417
SduI	GDGCHC	9 cut(s)	1411, 2020, 2442, 2899, 3110, 3288, 3412, 4090, 4148
SexAI	ACCWGGT	1 cut(s)	478
SfcI	CTRYAG	6 cut(s)	528, 826, 1452, 1881, 2166, 3468
SfuI	TTCGAA	1 cut(s)	2446
SinI	GGWCC	4 cut(s)	933, 1034, 2762, 2917
SmiMI	CAYNNNNRTG	1 cut(s)	4224
SnaBI	TACGTA	1 cut(s)	866
SpeI	ACTAGT	1 cut(s)	1801
SphI	GCATGC	2 cut(s)	1815, 3254
SsiI	CCGC	7 cut(s)	706, 1090, 2755, 2924, 3167, 4062, 4084
SspI	AATATT	2 cut(s)	3742, 3774
SspMI	CTAG	8 cut(s)	275, 299, 1802, 2270, 3183, 3303, 3315, 3588
SstI	GAGCTC	1 cut(s)	4148
StyD4I	CCNGG	8 cut(s)	118, 400, 478, 1762, 2610, 2857, 3287, 3415
StyI	CCWWGG	6 cut(s)	213, 653, 1196, 2627, 3337, 3371
TaiI	ACGT	2 cut(s)	868, 2539
TaqI	TCGA	8 cut(s)	1246, 1428, 1466, 1495, 2253, 2446, 2811, 4142
TaqII	GACCGA	1 cut(s)	1019
TatI	WGTACW	5 cut(s)	671, 1740, 2212, 2420, 3079
TfiI	GAWTC	9 cut(s)	109, 1862, 2044, 2227, 2448, 2588, 3235, 3452, 3680
TseFI	GTSAC	2 cut(s)	1268, 3608
Tsp45I	GTSAC	2 cut(s)	1268, 3608
TspGWI	ACGGA	6 cut(s)	102, 2314, 3391, 3395, 3627, 4383
Tth111I	GACNNNGTC	1 cut(s)	1212
Vha464I	CTTAAG	2 cut(s)	3424, 4453
VpaK11BI	GGWCC	4 cut(s)	933, 1034, 2762, 2917
VspI	ATTAAT	2 cut(s)	3525, 3650
XagI	CCTNNNNNAGG	3 cut(s)	405, 492, 4417
XapI	RAATTY	6 cut(s)	688, 779, 1043, 1430, 2093, 3824
XceI	RCATGY	8 cut(s)	1394, 1815, 2510, 2678, 2786, 3254, 3626, 4344
XcmI	CCANNNNNNNNNTGG	4 cut(s)	157, 1430, 2737, 3315
XmiI	GTMKAC	1 cut(s)	3803
XmnI	GAANNNNTTC	5 cut(s)	222, 948, 3067, 4027, 4381
XspI	CTAG	8 cut(s)	275, 299, 1802, 2270, 3183, 3303, 3315, 3588
ZrmI	AGTACT	1 cut(s)	2422
Zsp2I	ATGCAT	6 cut(s)	950, 1375, 1776, 1817, 3252, 3816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.