Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0015983

Physical Location & Seq

Genomic Coordinates Reverse (-)

15226654 .. 15233577

Gene Structure 6924 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007272

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4959 bp

ATGGAGCACCAAATGTGTTTCGACTGCCTCCAGCGCCGGATTGAGTCGGAGTTCTCCGGCAAACTCGTCTTCATCCACGGACTCTCCGATTCCGCTTTTCCGTTCGGCTCAAACGCCGTTGTTCAGTTGTCTAGTTCAAGTGGAGAAGCTGCTTCAGCTCCTCAATTCTTGCTCAAGTATCTACCCAGTCATCACCAGGATTGCTTGACCAAATTTGTGGATGAGTATATACTGGATGATGCTGATGTAAATCGAGATGGAGAGGAAGTCAGTTCTGAAATTTGCACTGATGAAAAGCCGAAATTGGGTTCTTTACCGACTGAGTCGCGTTGTTTATCAAATGGTGGGAAAGCATTGGCACAGAGTTCTAATTGTAACCATTCCAGTAGGTTTTCTTGCTCAAGGGTAATCAGTGCCTTGGCACCGGTTACTGAAGTTGGCCTTTCTTCAGACTCCATCGAAGAGCTTGCTTCCAGCTTTTTGTCTGGGTCTATGGAAGATCACATATTGAACTCACTTAGTCTCTTGATTGAAGGGAAAGCTTCGGGAAGAGATAGTCTCAACTTTCTTAATTTACTTGGGGTACCATCATTTGATGAGAATCCGTTTCCTGGCTCCTTGAGGCATCCAAATATCGCTCCCATACTTGGGATGGTTAAGACATCTGGTTATATTGATGTAGTTCTTCCCAAAGCTCCATATACCTTGGAAAACATTCTCCATTATAGCCCTGATGCCCTAAAGTCTGATTGGCATATAAGGTTTCTAGTGTATCAGTTGCTCTCAGCTCTAGCTTATATTCATGGTTTAGGGGTTGCTCATGGCAATATATGTCCGTCCAATGTAATGCTTTCTGAGTCATGCTGGTCTTGGCTGTGTGTATGTGATAAGCCTCGGTTAGGGTTTAATTCAAGTTCAAGAGGTAATGGGAATACAATTACTGCACCTGAAAAGGTTGGTTGCTCTGTACCAGGTTGTCCTTCTCAAGGTCTTTATGCTGATTTGAAGCTTTCCCCGTCTATAGACTGGCATCGGGACTTTAATCAGTGGTGGAGGGGAGAGATCAGTAATTTTGAATATCTACTCATCTTGAATAGATTAGCTGGGAGAAGGTGGGGTGATCACACATTTCATACAGTGATGCCATGGGTAATAGATTTTAGTACAAAACCTGATGAGAATTCTGATGCAGGGTGGCGGGATTTGAGTAAGAGTAAATGGAGGTTGGCTAAAGGTGATGAGCAGTTGGACTTCACCTATTCAACCTCAGAATTCCCACATCATGTTTCTGATGAGTGTCTTTCTGAATTGGCTGTCTGCAGCTATAAAGCAAGGAGGTTGCCTTTGAGTGTTCTACGAATGGCTGTTCGCGCAGTCTATGAACCTAATGAATATCCTTCTACAATGCAAAGACTCTATCAATGGACCCCTGATGAATGCATTCCGGAGTTTTACTGCGATCCCCAAGTTTTCAATTCACTTCATGCTGGTATGATCGACTTAGCTGTACCTTCATGGGCAGATGGTCCTGAGGAATTCATCAAATTGCATCGTGAAGCATTAGAAAGCGATCGGGTTTCCCGCCAACTTCATCACTGGATTGATATCACCTTTGGTTACAAGATGTCAGGTCAGGCAGCTGTTGCAGCAAAGAATGTAATGCTCCCTTCTTCAGAGTCCATGATGCCAAGGTCAGTGGGACGCCGTCAGCTTTTTACTGAACCTCATCCTATGCGTCGATGTGCAATGTGGAAGCCATGTGATAGCACCAATGAGTCAGCTTCATATCAAGGAGAAATGAATGAATTAAGGAGCGAAAGTTCTGTCCTATCTGACATGGCTTATTTGCAACTATTAGAAGATGCATCAGCCTTTTGCGAACATGCTATGGAATTGAGTGCCTTCTATGGGTACCATCTCGAATTTGGCAAGGACATTGCTCCTGTAGAAGAACAATCGAGTGAGAATGTTAAGAACAGCATATCTCGATCATCTGACACTAAGCAAAACCAGTGGTTGCCATTACAAATTGATACAAATTATCTTCTTGAGCATATTAAGGTGGAGGATGAAGGCTCCACTGGATATCAAGAGCTATTGCTTTGGAAGCAGAAATCATCTTGTTTGAAGACATTTACTGAAGATGTTGCAAGGGACATATTTTCTATTGGTTGTCTCTTAGCAGAACTTTATTTGAGGAGGCCAATTTTCGATCCAGCCTCACTGACCATGTACTTGGACAGTGGTCTCTTACCTGGAGTAATCCATGAACTTCCTCCTCACACCAAATTACTGGTTGAAGCATGCATTCAAAAGGATTGCATGAGGAGGCCATCAGTGAAGTCTCTTTTGGAATCACCTTACTTTCCTTCTACAGTCAAGGCGTCCTACTTGTTTCTTGCCCCACTCCATCTTCGGGCTAAAGATGGATCCTGCCTCCATTATGCTGCAAAATATGCAAAGCAAGGAGTCCTCAAGGAAATGGGAAGATTTGCAGCTGAAATGTGTGCTCCTTTTTGCTTATCACTTGTGGTGATTCCTTTGTCAGATATTGAAGCAGAATGGGCTTATACACTACTAAAAGAATTTATTAAAAGTTTGACACCTAAAGCAGTAAAGACAATAGTCTTGCCTGCTATCCAGAGGATTTTACAGACTACAGGTTATTCACATCTAAAGGTTTCTATTCTGCAAGATTCATTTGTACAAGAGATATGGAATCGGGTTGGCAAACAAGCATATCTCGAAACCGTACATCCTCTAGTAATAGTGAACTTATATGCTGCTGCTCATAAGAGTTCATCAGCTGCTGCTTCCGTGCTGCTGCTCGGCTCAAGTGAAGAGCTTGGTATTCCTATTACTATTTATCAGACAATCTTACCTTTGATCCAGTGCTTTGGGAAAGGTCTAAGTACTGATGGAATTGATGTGCTGATTCGAATCGGTGGTCTATTGGGGGAAAGCTTCATAGTCAGACAAATGCTACCATTGCTAAAACATGTAGTTCGTTCGTGCATTGACATTTCACGTATGAATAAGCCTGAGCCTGTCCACAGCTGGAGTGCTTTTGCTCTTATTGATTCTTTAATGACAATAGATGGCCTGGTTGCATTCTTGCCAAGGGAGGTGGTTGTAAAGGAGCTAATTGAAGATAGAAGTTGCCTGCATGTTCTGGTTCTTATGCAAACAAGTTTGGAATATCGAGTGGTTCAGGTTGCTGCCACAACCCTTATGGTTGTTTGTCAGCGGATAGGACCAGATTTGACGGCATTACATGTTTTGCCACAACTGAAGGAGCTATTCGATGAGCTTGCTTTCTCACCAGAAACTGCTAATGCATCTACTTCCCCTGGCAGAAGGTCGAAGATTTCAAAGCTAAAAGACGGGGCTCTGATTGAAAGTCGTATGGACCTTGCGTTGCTTCTGTATCCTCCTTTTGCATCCCTTCTTGGGATAGAGAAGCTTCGTCAGTGTTGTGCAACATGGTTATTACTTGAGCGATATCTTCTACAATTTCATAATTGGAAGTGGGAACACACAGGAGAATTGTCGCGAAGTGGTTCAGACACTATACTCAATAAAAGAGCATACAGAAAGGGCTCAACTCCTGAATACAGTCCTGCTAAGCTGTTGCTTAATGGGGTTGGATGGTCAATTCCGCAATCTCAAGGAACTAGAGGTGCCAAAAACTTGATGCCTCAGAAACGGTTTCATGCAGTGCATCAGAGTCCAACAGAAATGCATGCAGCAGCAGCATCAAATTATAAATATGAGCCCTGGTTTTGGTTCCCTAGTCCAGCTGCTAGCTGGGATGGGCCTGATTTCCTTGGGCGTGCTGGGGGTGTGAAAGATGAACATCCATGGAAGATCAGAGCATCTGTTGTACACTCCGTCCGTGCTCATCCTGGAGCCTTAAGATATTTAGCCGTCTGTCCAGATGAATCCACGGTTTTTACTGCAGGGATTGGTGCAGGCTTCAGAGGAACTGTTCAGAAATGGGAGCTGAGTAGAATTAATTGTGTATCTGGATACTACGGCCATGAGGAGGTTGTGAACGATATTTGCATCTTGTCGTCTAGTAGAAGAGTTGCTTCCTGTGACGGAACAATACATGTCTGGAATAGCCGAACAGGGAAATTAATTTCAGTATTTTCCGAACCATCAGTGGATTCGGCACATATTGCAAGCCCTCCATCTTCTTCTTCTAGGGCCAGTGTTGACCAGAGTAATATTCTCAGTCCCAATTCAATGTCCAGTGGAATATTGACTGGTGCATTTGATGGGAGCCTGTATACTTGTATGCATCAAACTGAATTTGGTGAAACGCTTGTCGCTGGCACTGGAGATGGTTCTCTGAGGTTCATTGATGTTGTCCGAGGGGAAAAGCTGCATCTTTGGAAGGGGAATTATCTGGAGTCAGGCTTTCCTTCTATAGTTTCTACCTTATGCTCTTGTGGGTCTGACAAAATGCAACCAGATGGAGCTTCTTCACCATCTTGGATTGCAGCTGGAATGAGTTCTGGCCATTGTAGGTTATTTGATGCAAGGAGTGGAAATGTTATTACCTCTTGGCGGGCTCATGATGGATATGTTACAAAGTTGGCTGCACCAGAAGACTATTTGCTTGTGTCGAGCTCTCTTGACAAGACTTTAAGGATTTGGGACTTGAGGAGGAATGTGCCATCTCAGCCCACAATTCTTAGAGGTCATACAGATGGTGTATCTGCTTTCTCTTTATGGGGCCAAGATGTTATTTCAATTGCCAGAAATAAAATTGGACTGTCTTCTTTATCAAAATCCCCTGATGATGATGGACAGCAGGCCGTTACATGTCAAAGGCTGTACATGGCAGACCACGGAGCAAGAAGCTTTTCAGTGTTGTCAAGCATAAGCATTTTACCCTTCTCAAGGTTGTTCCTTGTTGGGACAGAAGATGGTTACTTAAGAATATGCTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006950 GO:0006952 GO:0006955 GO:0007034 GO:0008104 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0015031 GO:0015833 GO:0031461 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042742 GO:0042886 GO:0043207 GO:0044424 GO:0044464 GO:0045087 GO:0045184 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0080008 GO:0098542 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Beach Pkinase WD40

Protein Analysis

1653

Amino Acids

183.18

Weight (kDa)

6.13

Isoelectric Point (pI)

55.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	205 - 286	1.6e-07	Protein kinase domain
Beach	PF02138	348 - 558	2.1e-41	Beige/BEACH domain
WD40_Prp19	PF24814	1327 - 1559	2.6e-09	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	1363 - 1554	4.5e-07	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	1427 - 1575	1.1e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	1498 - 1581	5.1e-09	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	1501 - 1579	4.4e-07	WDR5 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18525
fragaria_vesca	FvH4_4g21250 FvH4_4g21250
malus_domestica	MD13G1143700.v1.1
prunus_persica	Prupe.1G108200_v2.0.a1
pyrus_communis	pycom13g12460
rosa_chinensis	RchiOBHm_Chr4g0426871
rosa_laevigata	RLG00000007272
rosa_multiflora	Rmu_sc0000953.1_g000015
rosa_roxburghii	Rroxscaffold_5G00368840
rosa_samantha	Rh4AG270500 Rh4BG276800 Rh4CG291500 Rh4DG274000
rosa_wichuraiana	Rw4G023470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3765
Acc65I	GGTACC	2 cut(s)	583, 1911
AccB1I	GGYRCC	4 cut(s)	421, 583, 1911, 3680
AccI	GTMKAC	1 cut(s)	4292
AccII	CGCG	3 cut(s)	328, 1371, 3553
AccIII	TCCGGA	1 cut(s)	1444
AciI	CCGC	6 cut(s)	93, 1198, 1582, 3247, 3659, 4573
AclWI	GGATC	5 cut(s)	1454, 2207, 2424, 2437, 2881
AcoI	YGGCCR	2 cut(s)	4036, 4522
AcsI	RAATTY	8 cut(s)	212, 279, 1180, 1271, 1535, 1922, 2585, 4313
AcuI	CTGAAG	7 cut(s)	138, 432, 453, 1656, 2160, 3311, 3961
AcyI	GRCGYC	2 cut(s)	1702, 2384
AflII	CTTAAG	2 cut(s)	3913, 4942
AflIII	ACRYGT	4 cut(s)	2998, 3274, 4111, 4829
AgeI	ACCGGT	1 cut(s)	424
AjnI	CCWGG	8 cut(s)	195, 610, 970, 2254, 3102, 3349, 3776, 3904
AjuI	GAANNNNNNNTTGG	2 cut(s)	2333, 2365
AloI	GAACNNNNNNTCC	2 cut(s)	1804, 1836
Alw21I	GWGCWC	4 cut(s)	9, 2512, 3901, 4637
Alw26I	GTCTC	5 cut(s)	527, 563, 2180, 2252, 2349
AlwI	GGATC	5 cut(s)	1454, 2207, 2424, 2437, 2881
AlwNI	CAGNNNCTG	2 cut(s)	2810, 3329
Aor13HI	TCCGGA	1 cut(s)	1444
ApoI	RAATTY	8 cut(s)	212, 279, 1180, 1271, 1535, 1922, 2585, 4313
ArsI	GACNNNNNNTTYG	4 cut(s)	1809, 1841, 2932, 2964
AseI	ATTAAT	2 cut(s)	4014, 4139
AsiGI	ACCGGT	1 cut(s)	424
Asp700I	GAANNNNTTC	5 cut(s)	714, 1440, 3559, 4516, 4870
Asp718I	GGTACC	2 cut(s)	583, 1911
AspLEI	GCGC	2 cut(s)	36, 1373
AspS9I	GGNCC	7 cut(s)	1425, 1526, 3254, 3409, 3815, 4209, 4741
AsuII	TTCGAA	1 cut(s)	2938
AsuNHI	GCTAGC	1 cut(s)	3803
AvaII	GGWCC	4 cut(s)	1425, 1526, 3254, 3409
AxyI	CCTNAGG	1 cut(s)	1530
BalI	TGGCCA	1 cut(s)	4524
BamHI	GGATCC	1 cut(s)	2429
BanI	GGYRCC	4 cut(s)	421, 583, 1911, 3680
BanII	GRGCYC	5 cut(s)	3391, 3602, 3777, 4579, 4637
BbsI	GAAGAC	4 cut(s)	61, 2135, 4620, 4776
Bbv12I	GWGCWC	4 cut(s)	9, 2512, 3901, 4637
BceAI	ACGGC	6 cut(s)	101, 1689, 3282, 3911, 4051, 4808
BcgI	CGANNNNNNTGC	4 cut(s)	449, 483, 3293, 3327
BciT130I	CCWGG	8 cut(s)	197, 612, 972, 2256, 3104, 3351, 3778, 3906
BciVI	GTATCC	2 cut(s)	3438, 4022
BclI	TGATCA	1 cut(s)	1120
BcoDI	GTCTC	5 cut(s)	527, 563, 2180, 2252, 2349
BfaI	CTAG	9 cut(s)	132, 767, 791, 2762, 3675, 3792, 3804, 4077, 4206
BfmI	CTRYAG	7 cut(s)	1020, 1318, 1944, 2373, 2658, 3957, 4431
BfoI	RGCGCY	1 cut(s)	37
BfrI	CTTAAG	2 cut(s)	3913, 4942
BfuI	GTATCC	2 cut(s)	3438, 4022
BlpI	GCTNAGC	1 cut(s)	3624
BmcAI	AGTACT	1 cut(s)	2914
Bme1390I	CCNGG	8 cut(s)	197, 612, 972, 2256, 3104, 3351, 3778, 3906
Bme18I	GGWCC	4 cut(s)	1425, 1526, 3254, 3409
BmgT120I	GGNCC	7 cut(s)	1425, 1526, 3254, 3409, 3815, 4209, 4741
BmrFI	CCNGG	8 cut(s)	197, 612, 972, 2256, 3104, 3351, 3778, 3906
BmrI	ACTGGG	1 cut(s)	180
BmtI	GCTAGC	1 cut(s)	3807
BmuI	ACTGGG	1 cut(s)	180
BoxI	GACNNNNGTC	2 cut(s)	2244, 2624
BpiI	GAAGAC	4 cut(s)	61, 2135, 4620, 4776
BplI	GAGNNNNNCTC	2 cut(s)	543, 575
BpmI	CTGGAG	6 cut(s)	14, 2277, 3079, 3927, 4362, 4433
Bpu10I	CCTNAGC	1 cut(s)	3042
Bpu1102I	GCTNAGC	1 cut(s)	3624
Bpu14I	TTCGAA	1 cut(s)	2938
BsaAI	YACGTR	1 cut(s)	3029
BsaBI	GATNNNNATC	5 cut(s)	249, 600, 1604, 2939, 3855
BsaHI	GRCGYC	2 cut(s)	1702, 2384
BsaI	GGTCTC	1 cut(s)	2252
BsaWI	WCCGGW	2 cut(s)	424, 1444
BsaXI	ACNNNNNCTCC	6 cut(s)	68, 98, 252, 282, 1804, 1834
Bse118I	RCCGGY	1 cut(s)	424
Bse21I	CCTNAGG	1 cut(s)	1530
Bse3DI	GCAATG	3 cut(s)	1752, 1935, 2987
Bse8I	GATNNNNATC	5 cut(s)	249, 600, 1604, 2939, 3855
BseAI	TCCGGA	1 cut(s)	1444
BseBI	CCWGG	8 cut(s)	197, 612, 972, 2256, 3104, 3351, 3778, 3906
BseJI	GATNNNNATC	5 cut(s)	249, 600, 1604, 2939, 3855
BseMI	GCAATG	3 cut(s)	1752, 1935, 2987
BseRI	GAGGAG	6 cut(s)	150, 2212, 2268, 2341, 4058, 4684
BseYI	CCCAGC	3 cut(s)	1103, 3807, 3836
BsgI	GTGCAG	3 cut(s)	927, 3990, 4590
Bsh1236I	CGCG	3 cut(s)	328, 1371, 3553
Bsh1285I	CGRYCG	1 cut(s)	1573
BshNI	GGYRCC	4 cut(s)	421, 583, 1911, 3680
BshTI	ACCGGT	1 cut(s)	424
BsiEI	CGRYCG	1 cut(s)	1573
BsiHKAI	GWGCWC	4 cut(s)	9, 2512, 3901, 4637
BsiSI	CCGG	4 cut(s)	37, 57, 425, 1445
BslFI	GGGAC	6 cut(s)	1049, 1713, 2168, 4224, 4676, 4939
BsmAI	GTCTC	5 cut(s)	527, 563, 2180, 2252, 2349
BsmFI	GGGAC	6 cut(s)	1049, 1713, 2168, 4224, 4676, 4939
BsmI	GAATGC	4 cut(s)	1440, 1442, 2307, 3110
Bso31I	GGTCTC	1 cut(s)	2252
Bsp119I	TTCGAA	1 cut(s)	2938
Bsp1286I	GDGCHC	8 cut(s)	9, 2512, 3391, 3602, 3777, 3901, 4579, 4637
Bsp13I	TCCGGA	1 cut(s)	1444
Bsp1407I	TGTACA	3 cut(s)	2704, 3883, 4842
Bsp1720I	GCTNAGC	1 cut(s)	3624
Bsp19I	CCATGG	2 cut(s)	1145, 3860
Bsp68I	TCGCGA	1 cut(s)	3553
BspACI	CCGC	6 cut(s)	93, 1198, 1582, 3247, 3659, 4573
BspEI	TCCGGA	1 cut(s)	1444
BspFNI	CGCG	3 cut(s)	328, 1371, 3553
BspHI	TCATGA	1 cut(s)	4579
BspMAI	CTGCAG	2 cut(s)	1322, 3961
BspOI	GCTAGC	1 cut(s)	3807
BspPI	GGATC	5 cut(s)	1454, 2207, 2424, 2437, 2881
BspQI	GCTCTTC	2 cut(s)	456, 2835
BspT104I	TTCGAA	1 cut(s)	2938
BspT107I	GGYRCC	4 cut(s)	421, 583, 1911, 3680
BspTI	CTTAAG	2 cut(s)	3913, 4942
BspTNI	GGTCTC	1 cut(s)	2252
BsrDI	GCAATG	3 cut(s)	1752, 1935, 2987
BsrFI	RCCGGY	1 cut(s)	424
BsrGI	TGTACA	3 cut(s)	2704, 3883, 4842
BssAI	RCCGGY	1 cut(s)	424
BssNAI	GTATAC	1 cut(s)	4293
BssNI	GRCGYC	2 cut(s)	1702, 2384
BssT1I	CCWWGG	7 cut(s)	417, 705, 1145, 1688, 3119, 3826, 3860
Bst1107I	GTATAC	1 cut(s)	4293
Bst2UI	CCWGG	8 cut(s)	197, 612, 972, 2256, 3104, 3351, 3778, 3906
Bst4CI	ACNGT	9 cut(s)	1138, 2243, 2377, 2752, 3617, 3708, 3949, 3988, 4782
Bst6I	CTCTTC	4 cut(s)	456, 544, 2835, 4078
BstACI	GRCGYC	2 cut(s)	1702, 2384
BstAFI	CTTAAG	2 cut(s)	3913, 4942
BstAPI	GCANNNNNTGC	3 cut(s)	1643, 2456, 4181
BstAUI	TGTACA	3 cut(s)	2704, 3883, 4842
BstBAI	YACGTR	1 cut(s)	3029
BstBI	TTCGAA	1 cut(s)	2938
BstDSI	CCRYGG	5 cut(s)	76, 1145, 3860, 3945, 4855
BstENI	CCTNNNNNAGG	3 cut(s)	897, 984, 4906
BstFNI	CGCG	3 cut(s)	328, 1371, 3553
BstH2I	RGCGCY	1 cut(s)	37
BstHHI	GCGC	2 cut(s)	36, 1373
BstMAI	GTCTC	5 cut(s)	527, 563, 2180, 2252, 2349
BstMCI	CGRYCG	1 cut(s)	1573
BstNI	CCWGG	8 cut(s)	197, 612, 972, 2256, 3104, 3351, 3778, 3906
BstNSI	RCATGY	8 cut(s)	1886, 2307, 3002, 3170, 3278, 3746, 4115, 4833
BstPAI	GACNNNNGTC	2 cut(s)	2244, 2624
BstSCI	CCNGG	8 cut(s)	195, 610, 970, 2254, 3102, 3349, 3776, 3904
BstSFI	CTRYAG	7 cut(s)	1020, 1318, 1944, 2373, 2658, 3957, 4431
BstUI	CGCG	3 cut(s)	328, 1371, 3553
BstV2I	GAAGAC	4 cut(s)	61, 2135, 4620, 4776
BstX2I	RGATCY	1 cut(s)	2429
BstXI	CCANNNNNNTGG	4 cut(s)	217, 2236, 2293, 2897
BstYI	RGATCY	1 cut(s)	2429
BstZ17I	GTATAC	1 cut(s)	4293
Bsu36I	CCTNAGG	1 cut(s)	1530
BsuI	GTATCC	2 cut(s)	3438, 4022
BtgI	CCRYGG	5 cut(s)	76, 1145, 3860, 3945, 4855
BtsI	GCAGTG	1 cut(s)	3723
BtuMI	TCGCGA	1 cut(s)	3553
CaiI	CAGNNNCTG	2 cut(s)	2810, 3329
CciI	TCATGA	1 cut(s)	4579
CfoI	GCGC	2 cut(s)	36, 1373
Cfr10I	RCCGGY	1 cut(s)	424
Cfr13I	GGNCC	7 cut(s)	1425, 1526, 3254, 3409, 3815, 4209, 4741
CseI	GACGC	3 cut(s)	1710, 1724, 2373
CsiI	ACCWGGT	1 cut(s)	970
CspAI	ACCGGT	1 cut(s)	424
CspCI	CAANNNNNGTGG	6 cut(s)	1677, 1712, 1994, 2029, 3108, 3143
EaeI	YGGCCR	2 cut(s)	4036, 4522
Eam1104I	CTCTTC	4 cut(s)	456, 544, 2835, 4078
EarI	CTCTTC	4 cut(s)	456, 544, 2835, 4078
Ecl136II	GAGCTC	1 cut(s)	4635
Eco130I	CCWWGG	7 cut(s)	417, 705, 1145, 1688, 3119, 3826, 3860
Eco24I	GRGCYC	5 cut(s)	3391, 3602, 3777, 4579, 4637
Eco31I	GGTCTC	1 cut(s)	2252
Eco32I	GATATC	3 cut(s)	1606, 2087, 3503
Eco47I	GGWCC	4 cut(s)	1425, 1526, 3254, 3409
Eco53kI	GAGCTC	1 cut(s)	4635
Eco57I	CTGAAG	7 cut(s)	138, 432, 453, 1656, 2160, 3311, 3961
Eco81I	CCTNAGG	1 cut(s)	1530
EcoICRI	GAGCTC	1 cut(s)	4635
EcoNI	CCTNNNNNAGG	3 cut(s)	897, 984, 4906
EcoRI	GAATTC	3 cut(s)	1180, 1271, 1535
EcoRII	CCWGG	8 cut(s)	195, 610, 970, 2254, 3102, 3349, 3776, 3904
EcoRV	GATATC	3 cut(s)	1606, 2087, 3503
EcoT14I	CCWWGG	7 cut(s)	417, 705, 1145, 1688, 3119, 3826, 3860
EcoT22I	ATGCAT	6 cut(s)	1442, 1867, 2309, 3340, 3744, 4305
EcoT38I	GRGCYC	5 cut(s)	3391, 3602, 3777, 4579, 4637
ErhI	CCWWGG	7 cut(s)	417, 705, 1145, 1688, 3119, 3826, 3860
FalI	AAGNNNNNCTT	4 cut(s)	426, 458, 4075, 4107
FaqI	GGGAC	6 cut(s)	1049, 1713, 2168, 4224, 4676, 4939
FauI	CCCGC	3 cut(s)	1191, 1589, 4566
FbaI	TGATCA	1 cut(s)	1120
FblI	GTMKAC	1 cut(s)	4292
FriOI	GRGCYC	5 cut(s)	3391, 3602, 3777, 4579, 4637
FspBI	CTAG	9 cut(s)	132, 767, 791, 2762, 3675, 3792, 3804, 4077, 4206
GlaI	GCGC	2 cut(s)	35, 1372
GsaI	CCCAGC	3 cut(s)	1107, 3811, 3840
GsuI	CTGGAG	6 cut(s)	14, 2277, 3079, 3927, 4362, 4433
HaeII	RGCGCY	1 cut(s)	37
HapII	CCGG	4 cut(s)	37, 57, 425, 1445
HgaI	GACGC	3 cut(s)	1710, 1724, 2373
HhaI	GCGC	2 cut(s)	36, 1373
Hin1I	GRCGYC	2 cut(s)	1702, 2384
Hin6I	GCGC	2 cut(s)	34, 1371
HinP1I	GCGC	2 cut(s)	34, 1371
HincII	GTYRAC	1 cut(s)	4219
HindII	GTYRAC	1 cut(s)	4219
HindIII	AAGCTT	5 cut(s)	540, 1007, 2962, 3461, 4867
HpaII	CCGG	4 cut(s)	37, 57, 425, 1445
Hpy166II	GTNNAC	6 cut(s)	2773, 3052, 3886, 4054, 4219, 4293
Hpy8I	GTNNAC	6 cut(s)	2773, 3052, 3886, 4054, 4219, 4293
Hpy99I	CGWCG	1 cut(s)	1740
HpyCH4III	ACNGT	9 cut(s)	1138, 2243, 2377, 2752, 3617, 3708, 3949, 3988, 4782
HpyCH4IV	ACGT	1 cut(s)	3028
HpySE526I	ACGT	1 cut(s)	3028
Hsp92I	GRCGYC	2 cut(s)	1702, 2384
HspAI	GCGC	2 cut(s)	34, 1371
Kpn2I	TCCGGA	1 cut(s)	1444
KpnI	GGTACC	2 cut(s)	587, 1915
Ksp22I	TGATCA	1 cut(s)	1120
LguI	GCTCTTC	2 cut(s)	456, 2835
MabI	ACCWGGT	1 cut(s)	970
MaeI	CTAG	9 cut(s)	132, 767, 791, 2762, 3675, 3792, 3804, 4077, 4206
MaeII	ACGT	1 cut(s)	3028
MaeIII	GTNAC	7 cut(s)	374, 427, 1616, 4097, 4591, 4825, 4937
MfeI	CAATTG	1 cut(s)	4758
MflI	RGATCY	1 cut(s)	2429
MhlI	GDGCHC	8 cut(s)	9, 2512, 3391, 3602, 3777, 3901, 4579, 4637
MlsI	TGGCCA	1 cut(s)	4524
MluNI	TGGCCA	1 cut(s)	4524
MmeI	TCCRAC	4 cut(s)	27, 1227, 3625, 3755
Mox20I	TGGCCA	1 cut(s)	4524
Mph1103I	ATGCAT	6 cut(s)	1442, 1867, 2309, 3340, 3744, 4305
MroI	TCCGGA	1 cut(s)	1444
MroXI	GAANNNNTTC	5 cut(s)	714, 1440, 3559, 4516, 4870
MscI	TGGCCA	1 cut(s)	4524
MslI	CAYNNNNRTG	1 cut(s)	4713
Msp20I	TGGCCA	1 cut(s)	4524
MspA1I	CMGCKG	7 cut(s)	1640, 2498, 2807, 3057, 3247, 3800, 4508
MspCI	CTTAAG	2 cut(s)	3913, 4942
MspI	CCGG	4 cut(s)	37, 57, 425, 1445
MspR9I	CCNGG	8 cut(s)	197, 612, 972, 2256, 3104, 3351, 3778, 3906
MunI	CAATTG	1 cut(s)	4758
Mva1269I	GAATGC	4 cut(s)	1440, 1442, 2307, 3110
MvaI	CCWGG	8 cut(s)	197, 612, 972, 2256, 3104, 3351, 3778, 3906
MvnI	CGCG	3 cut(s)	328, 1371, 3553
NcoI	CCATGG	2 cut(s)	1145, 3860
NheI	GCTAGC	1 cut(s)	3803
NmeAIII	GCCGAG	1 cut(s)	2808
NmuCI	GTSAC	1 cut(s)	4097
NruI	TCGCGA	1 cut(s)	3553
NsiI	ATGCAT	6 cut(s)	1442, 1867, 2309, 3340, 3744, 4305
NspI	RCATGY	8 cut(s)	1886, 2307, 3002, 3170, 3278, 3746, 4115, 4833
NspV	TTCGAA	1 cut(s)	2938
PaeI	GCATGC	2 cut(s)	2307, 3746
PagI	TCATGA	1 cut(s)	4579
PciI	ACATGT	4 cut(s)	2998, 3274, 4111, 4829
PciSI	GCTCTTC	2 cut(s)	456, 2835
PcsI	WCGNNNNNNNCGW	1 cut(s)	84
PctI	GAATGC	4 cut(s)	1440, 1442, 2307, 3110
PdmI	GAANNNNTTC	5 cut(s)	714, 1440, 3559, 4516, 4870
PflFI	GACNNNGTC	2 cut(s)	322, 1704
PfoI	TCCNGGA	1 cut(s)	3904
PinAI	ACCGGT	1 cut(s)	424
Ple19I	CGATCG	1 cut(s)	1573
Ppu21I	YACGTR	1 cut(s)	3029
PscI	ACATGT	4 cut(s)	2998, 3274, 4111, 4829
PshAI	GACNNNNGTC	2 cut(s)	2244, 2624
PshBI	ATTAAT	2 cut(s)	4014, 4139
PsiI	TTATAA	1 cut(s)	3765
Psp124BI	GAGCTC	1 cut(s)	4637
Psp6I	CCWGG	8 cut(s)	195, 610, 970, 2254, 3102, 3349, 3776, 3904
PspFI	CCCAGC	3 cut(s)	1103, 3807, 3836
PspGI	CCWGG	8 cut(s)	195, 610, 970, 2254, 3102, 3349, 3776, 3904
PspPI	GGNCC	7 cut(s)	1425, 1526, 3254, 3409, 3815, 4209, 4741
PstI	CTGCAG	2 cut(s)	1322, 3961
PstNI	CAGNNNCTG	2 cut(s)	2810, 3329
PsuI	RGATCY	1 cut(s)	2429
PsyI	GACNNNGTC	2 cut(s)	322, 1704
PvuI	CGATCG	1 cut(s)	1573
PvuII	CAGCTG	6 cut(s)	1640, 2498, 2807, 3057, 3800, 4508
RruI	TCGCGA	1 cut(s)	3553
RseI	CAYNNNNRTG	1 cut(s)	4713
SacI	GAGCTC	1 cut(s)	4637
SapI	GCTCTTC	2 cut(s)	456, 2835
Sau96I	GGNCC	7 cut(s)	1425, 1526, 3254, 3409, 3815, 4209, 4741
ScaI	AGTACT	1 cut(s)	2914
ScrFI	CCNGG	8 cut(s)	197, 612, 972, 2256, 3104, 3351, 3778, 3906
SduI	GDGCHC	8 cut(s)	9, 2512, 3391, 3602, 3777, 3901, 4579, 4637
SexAI	ACCWGGT	1 cut(s)	970
SfcI	CTRYAG	7 cut(s)	1020, 1318, 1944, 2373, 2658, 3957, 4431
SfuI	TTCGAA	1 cut(s)	2938
SinI	GGWCC	4 cut(s)	1425, 1526, 3254, 3409
SmiMI	CAYNNNNRTG	1 cut(s)	4713
SphI	GCATGC	2 cut(s)	2307, 3746
SsiI	CCGC	6 cut(s)	93, 1198, 1582, 3247, 3659, 4573
SspI	AATATT	2 cut(s)	4231, 4263
SspMI	CTAG	9 cut(s)	132, 767, 791, 2762, 3675, 3792, 3804, 4077, 4206
SstI	GAGCTC	1 cut(s)	4637
StyD4I	CCNGG	8 cut(s)	195, 610, 970, 2254, 3102, 3349, 3776, 3904
StyI	CCWWGG	7 cut(s)	417, 705, 1145, 1688, 3119, 3826, 3860
TaaI	ACNGT	9 cut(s)	1138, 2243, 2377, 2752, 3617, 3708, 3949, 3988, 4782
TaiI	ACGT	1 cut(s)	3031
TatI	WGTACW	6 cut(s)	1163, 2232, 2704, 2912, 3883, 4842
TseFI	GTSAC	1 cut(s)	4097
Tsp45I	GTSAC	1 cut(s)	4097
TspGWI	ACGGA	9 cut(s)	90, 93, 594, 825, 2806, 3880, 3884, 4116, 4872
Tth111I	GACNNNGTC	2 cut(s)	322, 1704
Vha464I	CTTAAG	2 cut(s)	3913, 4942
VpaK11BI	GGWCC	4 cut(s)	1425, 1526, 3254, 3409
VspI	ATTAAT	2 cut(s)	4014, 4139
XagI	CCTNNNNNAGG	3 cut(s)	897, 984, 4906
XapI	RAATTY	8 cut(s)	212, 279, 1180, 1271, 1535, 1922, 2585, 4313
XceI	RCATGY	8 cut(s)	1886, 2307, 3002, 3170, 3278, 3746, 4115, 4833
XcmI	CCANNNNNNNNNTGG	4 cut(s)	649, 1922, 3229, 3804
XmiI	GTMKAC	1 cut(s)	4292
XmnI	GAANNNNTTC	5 cut(s)	714, 1440, 3559, 4516, 4870
XspI	CTAG	9 cut(s)	132, 767, 791, 2762, 3675, 3792, 3804, 4077, 4206
ZrmI	AGTACT	1 cut(s)	2914
Zsp2I	ATGCAT	6 cut(s)	1442, 1867, 2309, 3340, 3744, 4305

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.