MD13G1168500.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010022
Physical Location & Seq
Reverse (-)
13630857 .. 13636161
5305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1168500.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGGAGGGCTCTGCGGCAGAAGCAGCGAAGAAGCGGCAGGAGTTTGCATTGGCATCCGCCTCCGAGCTCGCTTCGTCGTCGTCTTCTTTTACTCTGGCACCGGCGGTGGCTCGGTTCAGCGCCGAAGACGGAGTTGCAGAGCTCCGGTTTCAGCAGGAGGCTGAGTCCGATGCCCGCCTCAATGTCGATCTCCGAACTGCTAAGCTATTCAAGTTAGGTCCGGTGCAGTCGGTTTGCATAACGGAAGGTTCTGATACCGACAAAGAGAAATCGTATTCAAGGGGAGTCACTATTCGTTTTAATAACGAGGAGGAGAGTGCAGCCTTCCATCATGCATTTGAGCAATGGAAGAAGGACCTTGTTGATCAAGGACAAAAGTTACCAAATGGAGAACTATCATCTACCAAAAGCAAGTTTGACGACAAAATAGAGCCGTCTTCTGCTAAAATGTATTTCCATTACTACGGACAATTGCTACATCAACAGAACATGTTACAGGATTATGTTAGGACAGGAACCTATTATGCTGCAGTCATAGAAAATCGTGTGGATTTTAATGGTCGTGTGGTTGTTGATGTTGGTGCTGGCAGTGGTATTTTGTCATTATTTGCTGCTCAGGCTGGTGCTAAACATGTTTATGCTGTGGAAGCATCTGAAATGGCAGAATATGCACGCAAGCTAATTGCAGGGAACCCTTCACTGGGTGAAAGGATAACTGTAATCAAAGGTAAAATTGAGGAGGTTGAATTGCCAGAGAAAGCAGATATCCTGATCTCAGAGCCAATGGGCACTTTGTTAATTAATGAAAGAATGCTGGAAACCTATGTGATTGCAAGAGATCGGTTTCTTCAACCAAATGGGAAAATGTTCCCCACATTGGGAAGGATTCACATGGCACCTTTCAGTGATGAATATTTGTTTGTTGAAATAGCAAATAAGGCCCTCTTCTGGCAGCAACAAAATTATTATGGAGTTGATTTGCAACCGTTGTATGGATCTGCATTCAAGGGATATTTTTCACAGCCTGTGGTAGATGCCTTTGATCCAAGATTACTGGTGGCTCCTTCCATGTCTCATGTGATTGACTTTACCAAAACAAGCGAAGAGGACTTGTATGAAATCGACATACCTTTACGGTTTACAGCTGCTGTAGGCACTAGAGTGCATGGGTTAGCATGCTGGTTCGATGTCTTGTTTGATGGGAGTACCGTACAAAGGTGGCTTACCACCGCGCCTGGTGCACCAACAACCCATTGGTACCAATTACGCTGCGTTCTCTCTCAGCCAATTTATGTGATGGCAGGACAAGAAATCACTGGCCGACTCCACATGGTTGCCCACAATTCTCAGAGTTACACAATATATCTCACTTTGGCAGCTAAAATGTGGGGCCCTGGGGCTGAGCTAGGAGGAATAATTCAGCAGTCTTCTTGTAAACTTGATCTCAAAGAGCCCTACTATAGAATGTCGCAACCGCAAGCCTGCACAATTGCCCAAGATCAGCAACCACATCAGCAAATACAGACACAGGATTTGGACGATTCTGAATTTATTCCACAACAACCCTCACCGACTTCAGGCTCAAAGATCTTACTGGAAGAATTTGCCAAATCCGCGGGGGCTTGGAAGAACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008469 GO:0008757 GO:0009628 GO:0009651 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009987 GO:0010035 GO:0010038 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016273 GO:0016274 GO:0016277 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019919 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032259 GO:0032501 GO:0032502 GO:0034969 GO:0034970 GO:0034971 GO:0034972 GO:0035241 GO:0035242 GO:0035246 GO:0035247 GO:0036211 GO:0042054 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046686 GO:0046982 GO:0046983 GO:0048518 GO:0048522 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0140096 GO:1901564 GO:1902680 GO:1902882 GO:1902884 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01522 map01522
Pfam Domains
Protein Families
Methyltransf_25 PrmA

Protein Analysis

546

Amino Acids

60.72

Weight (kDa)

5.31

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_PRMT_N PF25350 13 - 118 7.8e-44 Protein arginine methyltransferase N-terminal PH domain
PrmA PF06325 184 - 260 1.1e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_25 PF13649 190 - 287 3.6e-07 Methyltransferase domain
PRMT_C PF22528 296 - 450 1.3e-20 Arginine methyltransferase oligomerization subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1257
AccB1I GGYRCC 3 cut(s) 97, 895, 1257
AccII CGCG 2 cut(s) 1232, 1616
AciI CCGC 9 cut(s) 14, 34, 57, 104, 175, 1230, 1475, 1614, 1616
AclWI GGATC 2 cut(s) 1003, 1037
AcoI YGGCCR 1 cut(s) 1318
AcsI RAATTY 2 cut(s) 1547, 1601
AcuI CTGAAG 1 cut(s) 1560
AdeI CACNNNGTG 1 cut(s) 704
AfaI GTAC 3 cut(s) 1207, 1212, 1259
AfiI CCNNNNNNNGG 8 cut(s) 700, 701, 877, 878, 948, 992, 1215, 1577
AflIII ACRYGT 2 cut(s) 489, 631
AgsI TTSAA 6 cut(s) 211, 279, 746, 851, 926, 1006
AjnI CCWGG 2 cut(s) 1234, 1393
AleI CACNNNNGTG 1 cut(s) 1160
AluBI AGCT 7 cut(s) 67, 142, 205, 679, 1145, 1379, 1405
AluI AGCT 7 cut(s) 67, 142, 205, 679, 1145, 1379, 1405
Alw21I GWGCWC 3 cut(s) 69, 144, 1243
Alw26I GTCTC 1 cut(s) 1077
Alw44I GTGCAC 1 cut(s) 1239
AlwI GGATC 2 cut(s) 1003, 1037
AlwNI CAGNNNCTG 1 cut(s) 1148
AoxI GGCC 3 cut(s) 939, 1318, 1390
ApaI GGGCCC 1 cut(s) 1394
ApaLI GTGCAC 1 cut(s) 1239
ApeKI GCWGC 8 cut(s) 23, 320, 527, 611, 952, 1145, 1269, 1376
ApoI RAATTY 2 cut(s) 1547, 1601
ArsI GACNNNNNNTTYG 2 cut(s) 1516, 1548
AseI ATTAAT 1 cut(s) 801
Asp700I GAANNNNTTC 2 cut(s) 866, 1551
Asp718I GGTACC 1 cut(s) 1257
AspLEI GCGC 2 cut(s) 122, 1234
AspS9I GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
AsuHPI GGTGA 2 cut(s) 716, 1560
AvaII GGWCC 2 cut(s) 218, 355
BaeGI GKGCMC 3 cut(s) 791, 1243, 1394
BanI GGYRCC 3 cut(s) 97, 895, 1257
BanII GRGCYC 5 cut(s) 11, 69, 144, 1394, 1455
BbsI GAAGAC 4 cut(s) 75, 132, 429, 1419
Bbv12I GWGCWC 3 cut(s) 69, 144, 1243
BbvI GCAGC 8 cut(s) 35, 332, 514, 598, 964, 1132, 1256, 1388
BccI CCATC 3 cut(s) 336, 1193, 1291
BceAI ACGGC 1 cut(s) 418
BciT130I CCWGG 2 cut(s) 1236, 1395
BclI TGATCA 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 1077
BfaI CTAG 2 cut(s) 1158, 1406
BfmI CTRYAG 3 cut(s) 528, 1149, 1459
BfoI RGCGCY 1 cut(s) 123
BglII AGATCT 1 cut(s) 1587
BlpI GCTNAGC 2 cut(s) 201, 1401
Bme1390I CCNGG 2 cut(s) 1236, 1395
Bme18I GGWCC 2 cut(s) 218, 355
BmgT120I GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
BmiI GGNNCC 8 cut(s) 99, 517, 692, 897, 1062, 1259, 1391, 1392
BmrFI CCNGG 2 cut(s) 1236, 1395
BmrI ACTGGG 1 cut(s) 710
BmsI GCATC 4 cut(s) 62, 160, 659, 1024
BmuI ACTGGG 1 cut(s) 710
BpiI GAAGAC 4 cut(s) 75, 132, 429, 1419
Bpu10I CCTNAGC 1 cut(s) 615
Bpu1102I GCTNAGC 2 cut(s) 201, 1401
BsaJI CCNNGG 3 cut(s) 1393, 1394, 1614
BsaWI WCCGGW 2 cut(s) 144, 220
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 8 cut(s) 700, 701, 877, 878, 948, 992, 1215, 1577
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 4 cut(s) 705, 1059, 1321, 1599
Bse3DI GCAATG 1 cut(s) 350
BseBI CCWGG 2 cut(s) 1236, 1395
BseDI CCNNGG 3 cut(s) 1393, 1394, 1614
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 8 cut(s) 700, 701, 877, 878, 948, 992, 1215, 1577
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 6 cut(s) 153, 629, 789, 1295, 1361, 1392
BseNI ACTGG 4 cut(s) 705, 1059, 1321, 1599
BseRI GAGGAG 3 cut(s) 323, 326, 752
BseSI GKGCMC 3 cut(s) 791, 1243, 1394
BseXI GCAGC 8 cut(s) 35, 332, 514, 598, 964, 1132, 1256, 1388
BsgI GTGCAG 3 cut(s) 245, 339, 1468
Bsh1236I CGCG 2 cut(s) 1232, 1616
BshFI GGCC 3 cut(s) 941, 1320, 1392
BshNI GGYRCC 3 cut(s) 97, 895, 1257
BsiHKAI GWGCWC 3 cut(s) 69, 144, 1243
BsiSI CCGG 3 cut(s) 101, 145, 221
BslI CCNNNNNNNGG 8 cut(s) 700, 701, 877, 878, 948, 992, 1215, 1577
BsmAI GTCTC 1 cut(s) 1077
BsmI GAATGC 2 cut(s) 816, 1001
BsnI GGCC 3 cut(s) 941, 1320, 1392
Bsp120I GGGCCC 1 cut(s) 1390
Bsp1286I GDGCHC 7 cut(s) 11, 69, 144, 791, 1243, 1394, 1455
Bsp143I GATC 9 cut(s) 187, 364, 771, 838, 995, 1042, 1441, 1498, 1587
Bsp1720I GCTNAGC 2 cut(s) 201, 1401
BspACI CCGC 9 cut(s) 14, 34, 57, 104, 175, 1230, 1475, 1614, 1616
BspANI GGCC 3 cut(s) 941, 1320, 1392
BspCNI CTCAG 6 cut(s) 154, 628, 788, 1294, 1360, 1393
BspFNI CGCG 2 cut(s) 1232, 1616
BspLI GGNNCC 8 cut(s) 99, 517, 692, 897, 1062, 1259, 1391, 1392
BspMAI CTGCAG 1 cut(s) 532
BspPI GGATC 2 cut(s) 1003, 1037
BspT107I GGYRCC 3 cut(s) 97, 895, 1257
BsrDI GCAATG 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 4 cut(s) 705, 1059, 1321, 1599
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 3 cut(s) 1393, 1394, 1614
BssMI GATC 9 cut(s) 187, 364, 771, 838, 995, 1042, 1441, 1498, 1587
Bst2UI CCWGG 2 cut(s) 1236, 1395
Bst4CI ACNGT 4 cut(s) 718, 987, 1137, 1210
Bst6I CTCTTC 2 cut(s) 950, 1098
BstAPI GCANNNNNTGC 1 cut(s) 668
BstC8I GCNNGC 8 cut(s) 69, 175, 586, 673, 677, 1177, 1479, 1483
BstDEI CTNAG 7 cut(s) 162, 201, 615, 775, 1281, 1347, 1401
BstDSI CCRYGG 1 cut(s) 1614
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 2 cut(s) 1232, 1616
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 2 cut(s) 122, 1234
BstKTI GATC 9 cut(s) 190, 367, 774, 841, 998, 1045, 1444, 1501, 1590
BstMAI GTCTC 1 cut(s) 1077
BstMBI GATC 9 cut(s) 187, 364, 771, 838, 995, 1042, 1441, 1498, 1587
BstMWI GCNNNNNNNGC 8 cut(s) 20, 23, 617, 647, 668, 938, 1238, 1613
BstNI CCWGG 2 cut(s) 1236, 1395
BstNSI RCATGY 3 cut(s) 493, 635, 1179
BstSCI CCNGG 2 cut(s) 1234, 1393
BstSFI CTRYAG 3 cut(s) 528, 1149, 1459
BstSLI GKGCMC 3 cut(s) 791, 1243, 1394
BstUI CGCG 2 cut(s) 1232, 1616
BstV1I GCAGC 8 cut(s) 35, 332, 514, 598, 964, 1132, 1256, 1388
BstV2I GAAGAC 4 cut(s) 75, 132, 429, 1419
BstX2I RGATCY 2 cut(s) 995, 1587
BstYI RGATCY 2 cut(s) 995, 1587
BsuRI GGCC 3 cut(s) 941, 1320, 1392
BtgI CCRYGG 1 cut(s) 1614
BtsCI GGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 4 cut(s) 595, 698, 910, 1314
Cac8I GCNNGC 8 cut(s) 69, 175, 586, 673, 677, 1177, 1479, 1483
CaiI CAGNNNCTG 1 cut(s) 1148
CfoI GCGC 2 cut(s) 122, 1234
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
Cfr42I CCGCGG 1 cut(s) 1617
Csp6I GTAC 3 cut(s) 1206, 1211, 1258
CviAII CATG 9 cut(s) 332, 490, 632, 892, 1069, 1076, 1166, 1176, 1330
CviQI GTAC 3 cut(s) 1206, 1211, 1258
DdeI CTNAG 7 cut(s) 162, 201, 615, 775, 1281, 1347, 1401
DpnI GATC 9 cut(s) 189, 366, 773, 840, 997, 1044, 1443, 1500, 1589
DpnII GATC 9 cut(s) 187, 364, 771, 838, 995, 1042, 1441, 1498, 1587
DraIII CACNNNGTG 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 1318
Eam1104I CTCTTC 2 cut(s) 950, 1098
EarI CTCTTC 2 cut(s) 950, 1098
EciI GGCGGA 1 cut(s) 46
Ecl136II GAGCTC 2 cut(s) 67, 142
Eco24I GRGCYC 5 cut(s) 11, 69, 144, 1394, 1455
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 2 cut(s) 218, 355
Eco53kI GAGCTC 2 cut(s) 67, 142
Eco57I CTGAAG 1 cut(s) 1560
EcoICRI GAGCTC 2 cut(s) 67, 142
EcoO109I RGGNCCY 4 cut(s) 355, 940, 1390, 1391
EcoRII CCWGG 2 cut(s) 1234, 1393
EcoRV GATATC 1 cut(s) 766
EcoT22I ATGCAT 1 cut(s) 337
EcoT38I GRGCYC 5 cut(s) 11, 69, 144, 1394, 1455
FaeI CATG 9 cut(s) 335, 493, 635, 895, 1072, 1079, 1169, 1179, 1333
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FatI CATG 9 cut(s) 331, 489, 631, 891, 1068, 1075, 1165, 1175, 1329
FauI CCCGC 2 cut(s) 182, 1609
FbaI TGATCA 1 cut(s) 364
FokI GGATG 1 cut(s) 40
FriOI GRGCYC 5 cut(s) 11, 69, 144, 1394, 1455
FspBI CTAG 2 cut(s) 1158, 1406
GlaI GCGC 2 cut(s) 121, 1233
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 3 cut(s) 941, 1320, 1392
HapII CCGG 3 cut(s) 101, 145, 221
HhaI GCGC 2 cut(s) 122, 1234
Hin1II CATG 9 cut(s) 335, 493, 635, 895, 1072, 1079, 1169, 1179, 1333
Hin6I GCGC 2 cut(s) 120, 1232
HinP1I GCGC 2 cut(s) 120, 1232
HinfI GANTC 5 cut(s) 164, 285, 886, 1323, 1541
HpaII CCGG 3 cut(s) 101, 145, 221
HphI GGTGA 2 cut(s) 716, 1560
Hpy166II GTNNAC 3 cut(s) 1140, 1241, 1436
Hpy188I TCNGA 8 cut(s) 64, 169, 194, 253, 655, 778, 1350, 1546
Hpy188III TCNNGA 1 cut(s) 769
Hpy8I GTNNAC 3 cut(s) 1140, 1241, 1436
Hpy99I CGWCG 2 cut(s) 79, 82
HpyAV CCTTC 6 cut(s) 239, 334, 346, 705, 876, 1074
HpyCH4III ACNGT 4 cut(s) 718, 987, 1137, 1210
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 23, 617, 647, 668, 938, 1238, 1613
HpyF3I CTNAG 7 cut(s) 162, 201, 615, 775, 1281, 1347, 1401
Hsp92II CATG 9 cut(s) 335, 493, 635, 895, 1072, 1079, 1169, 1179, 1333
HspAI GCGC 2 cut(s) 120, 1232
KpnI GGTACC 1 cut(s) 1261
Ksp22I TGATCA 1 cut(s) 364
KspI CCGCGG 1 cut(s) 1617
Kzo9I GATC 9 cut(s) 187, 364, 771, 838, 995, 1042, 1441, 1498, 1587
LmnI GCTCC 2 cut(s) 147, 1066
Lsp1109I GCAGC 8 cut(s) 35, 332, 514, 598, 964, 1132, 1256, 1388
LweI GCATC 4 cut(s) 62, 160, 659, 1024
MaeI CTAG 2 cut(s) 1158, 1406
MaeIII GTNAC 4 cut(s) 286, 378, 492, 1352
MalI GATC 9 cut(s) 189, 366, 773, 840, 997, 1044, 1443, 1500, 1589
MboI GATC 9 cut(s) 187, 364, 771, 838, 995, 1042, 1441, 1498, 1587
MfeI CAATTG 2 cut(s) 470, 1488
MflI RGATCY 2 cut(s) 995, 1587
MhlI GDGCHC 7 cut(s) 11, 69, 144, 791, 1243, 1394, 1455
MlyI GAGTC 3 cut(s) 173, 294, 1317
Mph1103I ATGCAT 1 cut(s) 337
MroXI GAANNNNTTC 2 cut(s) 866, 1551
MseI TTAA 4 cut(s) 300, 555, 797, 801
MslI CAYNNNNRTG 2 cut(s) 636, 1160
MspA1I CMGCKG 2 cut(s) 1145, 1616
MspI CCGG 3 cut(s) 101, 145, 221
MspR9I CCNGG 2 cut(s) 1236, 1395
MunI CAATTG 2 cut(s) 470, 1488
Mva1269I GAATGC 2 cut(s) 816, 1001
MvaI CCWGG 2 cut(s) 1236, 1395
MvnI CGCG 2 cut(s) 1232, 1616
MwoI GCNNNNNNNGC 8 cut(s) 20, 23, 617, 647, 668, 938, 1238, 1613
NdeII GATC 9 cut(s) 187, 364, 771, 838, 995, 1042, 1441, 1498, 1587
NlaIII CATG 9 cut(s) 335, 493, 635, 895, 1072, 1079, 1169, 1179, 1333
NlaIV GGNNCC 8 cut(s) 99, 517, 692, 897, 1062, 1259, 1391, 1392
NmuCI GTSAC 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 3 cut(s) 493, 635, 1179
OliI CACNNNNGTG 1 cut(s) 1160
PacI TTAATTAA 1 cut(s) 801
PaeI GCATGC 1 cut(s) 1179
PasI CCCWGGG 1 cut(s) 1394
PciI ACATGT 2 cut(s) 489, 631
PctI GAATGC 2 cut(s) 816, 1001
PdmI GAANNNNTTC 2 cut(s) 866, 1551
PfeI GAWTC 2 cut(s) 886, 1541
PleI GAGTC 3 cut(s) 172, 293, 1317
PpsI GAGTC 3 cut(s) 172, 293, 1317
PpuMI RGGWCCY 1 cut(s) 355
PscI ACATGT 2 cut(s) 489, 631
PshBI ATTAAT 1 cut(s) 801
Psp124BI GAGCTC 2 cut(s) 69, 144
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 2 cut(s) 1234, 1393
PspGI CCWGG 2 cut(s) 1234, 1393
PspN4I GGNNCC 8 cut(s) 99, 517, 692, 897, 1062, 1259, 1391, 1392
PspOMI GGGCCC 1 cut(s) 1390
PspPI GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
PspPPI RGGWCCY 1 cut(s) 355
PstI CTGCAG 1 cut(s) 532
PstNI CAGNNNCTG 1 cut(s) 1148
PsuI RGATCY 2 cut(s) 995, 1587
PvuII CAGCTG 1 cut(s) 1145
RsaI GTAC 3 cut(s) 1207, 1212, 1259
RsaNI GTAC 3 cut(s) 1206, 1211, 1258
RseI CAYNNNNRTG 2 cut(s) 636, 1160
SacI GAGCTC 2 cut(s) 69, 144
SacII CCGCGG 1 cut(s) 1617
SaqAI TTAA 4 cut(s) 300, 555, 797, 801
Sau3AI GATC 9 cut(s) 187, 364, 771, 838, 995, 1042, 1441, 1498, 1587
Sau96I GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
SchI GAGTC 3 cut(s) 173, 294, 1317
ScrFI CCNGG 2 cut(s) 1236, 1395
SduI GDGCHC 7 cut(s) 11, 69, 144, 791, 1243, 1394, 1455
SfaNI GCATC 4 cut(s) 62, 160, 659, 1024
SfcI CTRYAG 3 cut(s) 528, 1149, 1459
Sfr303I CCGCGG 1 cut(s) 1617
SgrAI CRCCGGYG 1 cut(s) 100
SgrBI CCGCGG 1 cut(s) 1617
SinI GGWCC 2 cut(s) 218, 355
SmiMI CAYNNNNRTG 2 cut(s) 636, 1160
SphI GCATGC 1 cut(s) 1179
SsiI CCGC 9 cut(s) 14, 34, 57, 104, 175, 1230, 1475, 1614, 1616
SspI AATATT 1 cut(s) 914
SspMI CTAG 2 cut(s) 1158, 1406
SstI GAGCTC 2 cut(s) 69, 144
StyD4I CCNGG 2 cut(s) 1234, 1393
TaaI ACNGT 4 cut(s) 718, 987, 1137, 1210
TaqI TCGA 3 cut(s) 186, 1122, 1185
TauI GCSGC 2 cut(s) 17, 37
TfiI GAWTC 2 cut(s) 886, 1541
Tru1I TTAA 4 cut(s) 300, 555, 797, 801
Tru9I TTAA 4 cut(s) 300, 555, 797, 801
TscAI CASTG 4 cut(s) 595, 705, 910, 1321
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 8 cut(s) 23, 320, 527, 611, 952, 1145, 1269, 1376
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 3 cut(s) 819, 924, 1131
TspGWI ACGGA 3 cut(s) 144, 257, 480
TspRI CASTG 4 cut(s) 595, 705, 910, 1321
VneI GTGCAC 1 cut(s) 1239
VpaK11BI GGWCC 2 cut(s) 218, 355
VspI ATTAAT 1 cut(s) 801
XapI RAATTY 2 cut(s) 1547, 1601
XceI RCATGY 3 cut(s) 493, 635, 1179
XcmI CCANNNNNNNNNTGG 1 cut(s) 1251
XmnI GAANNNNTTC 2 cut(s) 866, 1551
XspI CTAG 2 cut(s) 1158, 1406
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.