MD16G1168900.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010022
Physical Location & Seq
Reverse (-)
14047574 .. 14053079
5506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1168900.v1.1.491

Sequence Viewer

Length: 1659 bp
ATGGAGGGCTCTGTAGCAGAAACGGCGAAGAAGCAGCAGGTGTTTGCTTTGGCATCCGCCTCCGAGCTCGCTTCGTCGTCGTCTTCTTCTACTCTTGCGCCGGCGCTGGCTCGGTTCAGCGCCGAAGACGGAGTTGCAGAGCTTCGGTTTCAGCAGGAGGCCGAGTCCGTTGCTCGTCTCAATGTCGATCTCCTAACTGCTAAGCTATTCAAGTTAGGTCCGGTGCAGTCAGTTTGCATAACGGAAGGTTCTGATACCGACAAAGAGAAATCGTATTCAAGGGGAGTCACTATTCATTTTAATAATGAGGTGGACAGTGGAGCCTTCCATCATGCATTTGAGCAATGGAAGAAGGACCTTGTTGATCAAGGACAAAAATTACCAAATGGAGAACTATCATCTACCAAAAGCAAGTTTGACGACAAAATAGAGCCATCTTCTGCTAAAATGTATTTCCATTACTATGGACAATTGCTGCATCAACAGAACATGTTACAAGATTATGTGAGGACAGGAACCTATTATGCTGCAGTCATAGAAAATCGTGTGGATTTTAATGGTCGTGTGGTTGTTGATGTTGGTGCTGGTAGTGGTATTTTGTCATTATTTGCTGCTCAGGCTGGTGCTAAACATGTTTATGCTGTGGAAGCATCTGAAATGGCAGAATATGCACGCAAGCTAATTGCAGGGAACCCATTACTGGGTCAAAGGATAACTGTAATCAAAGGTAAAGTCGAGGAGGTTGAATTGCCTGAGAAAGCAGATATCCTTATCTCGGAGCCAATGGGCACCTTGTTAATTAATGAAAGAATGCTGGAAACCTATGTGATTGCAAGGGATCGGTTTCTTCAGCCAAATGGAAAAATGTTCCCCACATTGGGAAGGATTCACATGGCACCTTTCAGTGATGAATATTTGTTTGTTGAAATTGCAAATAAGGCCCTCTTCTGGCAGCAACCAAATTATTATGGTGTTGATTTGCAACCATTGTATGGATCTGCATTCCAGGGATATTTTTCACAGCCAGTGGTAGATGCTTTTGATCCAAGATTACTGGTGGCTCCTTCAATGTCTCATGTGATTGACTTTACCAAAATAAGCGAAGAGGACTTGTATGAAATTGATATACCGCTACGGTTTACGGCTGCAGTAGGCACTAGAGTGCATGGGTTAGCATGCTGGTTCGATGTCTTGTTTGATGGGAGTACCGTACAAAGGTGGCTTACCACCGCACCTGGTGCACCAACCACCCATTGGTACCAATTACGTTGCGTTCTCGCTCAGCCAATTTATGTGATGGCAGGACAAGAAATCACTGGCCGACTCCACATGGTTGCCCACAATGCTCAAAGTTACACAATGTATCTCACATTGGCAGCTAAAATGTGGGGCCCTGGTGCCGAGCAAGGAGGAATAATTCAGCAATCTTCTTGTAAACTTGATCTCAAAGAGCCCTACTATAGAATGTCCCAACCGCAAGCGTACACAATTGCCCAAGATCCGCAACCACATCAGCAAATACAGACGCAGGATATACCCATTCATTCCCAGGATTTGGATGATTCTGAATTTTTTCCACAACCACCATCACCAACTTCAGGGACAAAGATCTTACTGGAAGAGTTTGCCAAATCCGCGGGGGCTTGGAAGAACGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008469 GO:0008757 GO:0009628 GO:0009651 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009987 GO:0010035 GO:0010038 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016273 GO:0016274 GO:0016277 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019919 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032259 GO:0032501 GO:0032502 GO:0034969 GO:0034970 GO:0034971 GO:0034972 GO:0035241 GO:0035242 GO:0035246 GO:0035247 GO:0036211 GO:0042054 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046686 GO:0046982 GO:0046983 GO:0048518 GO:0048522 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0140096 GO:1901564 GO:1902680 GO:1902882 GO:1902884 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01522 map01522
Pfam Domains
Protein Families
Methyltransf_25 PrmA

Protein Analysis

553

Amino Acids

61.35

Weight (kDa)

5.33

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_PRMT_N PF25350 12 - 118 1.1e-40 Protein arginine methyltransferase N-terminal PH domain
PrmA PF06325 184 - 260 1e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_25 PF13649 190 - 287 1.9e-07 Methyltransferase domain
PRMT_C PF22528 296 - 448 2.4e-20 Arginine methyltransferase oligomerization subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
Acc36I ACCTGC 1 cut(s) 28
Acc65I GGTACC 1 cut(s) 1257
AccB1I GGYRCC 4 cut(s) 788, 895, 1257, 1397
AccB7I CCANNNNNTGG 3 cut(s) 992, 1254, 1555
AccII CGCG 1 cut(s) 1637
AciI CCGC 7 cut(s) 57, 1130, 1230, 1475, 1502, 1635, 1637
AclI AACGTT 1 cut(s) 1653
AclWI GGATC 4 cut(s) 846, 1003, 1037, 1493
AcoI YGGCCR 1 cut(s) 1318
AcsI RAATTY 1 cut(s) 1568
AcuI CTGAAG 2 cut(s) 833, 1581
AdeI CACNNNGTG 1 cut(s) 1238
AfaI GTAC 4 cut(s) 1207, 1212, 1259, 1484
AflIII ACRYGT 2 cut(s) 489, 631
AgsI TTSAA 5 cut(s) 211, 279, 746, 926, 1068
AjnI CCWGG 4 cut(s) 1005, 1234, 1393, 1548
AleI CACNNNNGTG 1 cut(s) 1160
AluBI AGCT 5 cut(s) 67, 142, 205, 679, 1379
AluI AGCT 5 cut(s) 67, 142, 205, 679, 1379
Alw21I GWGCWC 2 cut(s) 69, 1243
Alw26I GTCTC 2 cut(s) 182, 1077
Alw44I GTGCAC 1 cut(s) 1239
AlwI GGATC 4 cut(s) 846, 1003, 1037, 1493
AoxI GGCC 4 cut(s) 159, 939, 1318, 1390
ApaI GGGCCC 1 cut(s) 1394
ApaLI GTGCAC 1 cut(s) 1239
ApeKI GCWGC 7 cut(s) 34, 475, 527, 611, 952, 1145, 1376
ApoI RAATTY 1 cut(s) 1568
ArsI GACNNNNNNTTYG 2 cut(s) 717, 749
AseI ATTAAT 1 cut(s) 801
Asp700I GAANNNNTTC 1 cut(s) 1572
Asp718I GGTACC 1 cut(s) 1257
AspLEI GCGC 3 cut(s) 100, 106, 122
AspS9I GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
AsuHPI GGTGA 1 cut(s) 1581
AvaII GGWCC 2 cut(s) 218, 355
BaeGI GKGCMC 3 cut(s) 791, 1243, 1394
BanI GGYRCC 4 cut(s) 788, 895, 1257, 1397
BanII GRGCYC 4 cut(s) 11, 69, 1394, 1455
BbsI GAAGAC 2 cut(s) 75, 132
Bbv12I GWGCWC 2 cut(s) 69, 1243
BbvI GCAGC 7 cut(s) 46, 462, 514, 598, 964, 1132, 1388
BccI CCATC 5 cut(s) 336, 442, 1193, 1291, 1594
BceAI ACGGC 2 cut(s) 39, 1158
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BciT130I CCWGG 4 cut(s) 1007, 1236, 1395, 1550
BclI TGATCA 1 cut(s) 364
BcoDI GTCTC 2 cut(s) 182, 1077
BfaI CTAG 1 cut(s) 1158
BfmI CTRYAG 4 cut(s) 12, 528, 1146, 1459
BfoI RGCGCY 2 cut(s) 107, 123
BfuAI ACCTGC 1 cut(s) 28
BglII AGATCT 1 cut(s) 1608
BisI GCNGC 7 cut(s) 35, 476, 528, 612, 953, 1146, 1377
BlpI GCTNAGC 2 cut(s) 201, 1281
BlsI GCNGC 7 cut(s) 36, 477, 529, 613, 954, 1147, 1378
Bme1390I CCNGG 4 cut(s) 1007, 1236, 1395, 1550
Bme18I GGWCC 2 cut(s) 218, 355
BmgT120I GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
BmrFI CCNGG 4 cut(s) 1007, 1236, 1395, 1550
BmrI ACTGGG 1 cut(s) 710
BmsI GCATC 4 cut(s) 62, 487, 659, 1024
BmuI ACTGGG 1 cut(s) 710
BpiI GAAGAC 2 cut(s) 75, 132
Bpu10I CCTNAGC 1 cut(s) 615
Bpu1102I GCTNAGC 2 cut(s) 201, 1281
BsaJI CCNNGG 4 cut(s) 1006, 1393, 1548, 1635
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 5 cut(s) 705, 1025, 1059, 1321, 1620
Bse3DI GCAATG 1 cut(s) 350
BseBI CCWGG 4 cut(s) 1007, 1236, 1395, 1550
BseDI CCNNGG 4 cut(s) 1006, 1393, 1548, 1635
BseGI GGATG 2 cut(s) 53, 1564
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 3 cut(s) 629, 744, 1295
BseNI ACTGG 5 cut(s) 705, 1025, 1059, 1321, 1620
BseRI GAGGAG 1 cut(s) 752
BseSI GKGCMC 3 cut(s) 791, 1243, 1394
BseXI GCAGC 7 cut(s) 46, 462, 514, 598, 964, 1132, 1388
BsgI GTGCAG 1 cut(s) 245
Bsh1236I CGCG 1 cut(s) 1637
BshFI GGCC 4 cut(s) 161, 941, 1320, 1392
BshNI GGYRCC 4 cut(s) 788, 895, 1257, 1397
BsiHKAI GWGCWC 2 cut(s) 69, 1243
BsiSI CCGG 2 cut(s) 101, 221
BslFI GGGAC 2 cut(s) 1453, 1615
BsmAI GTCTC 2 cut(s) 182, 1077
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 1453, 1615
BsmI GAATGC 2 cut(s) 816, 1001
BsnI GGCC 4 cut(s) 161, 941, 1320, 1392
Bsp120I GGGCCC 1 cut(s) 1390
Bsp1286I GDGCHC 6 cut(s) 11, 69, 791, 1243, 1394, 1455
Bsp143I GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1608
Bsp1720I GCTNAGC 2 cut(s) 201, 1281
BspACI CCGC 7 cut(s) 57, 1130, 1230, 1475, 1502, 1635, 1637
BspANI GGCC 4 cut(s) 161, 941, 1320, 1392
BspCNI CTCAG 3 cut(s) 628, 745, 1294
BspFNI CGCG 1 cut(s) 1637
BspMAI CTGCAG 2 cut(s) 532, 1150
BspMI ACCTGC 1 cut(s) 28
BspPI GGATC 4 cut(s) 846, 1003, 1037, 1493
BspT107I GGYRCC 4 cut(s) 788, 895, 1257, 1397
BsrDI GCAATG 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 5 cut(s) 705, 1025, 1059, 1321, 1620
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 4 cut(s) 1006, 1393, 1548, 1635
BssMI GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1608
Bst2UI CCWGG 4 cut(s) 1007, 1236, 1395, 1550
Bst4CI ACNGT 4 cut(s) 317, 718, 1137, 1210
Bst6I CTCTTC 3 cut(s) 950, 1098, 1614
BstAPI GCANNNNNTGC 2 cut(s) 668, 1238
BstC8I GCNNGC 7 cut(s) 69, 102, 108, 673, 677, 1177, 1479
BstDEI CTNAG 4 cut(s) 201, 615, 753, 1281
BstDSI CCRYGG 1 cut(s) 1635
BstF5I GGATG 2 cut(s) 53, 1564
BstFNI CGCG 1 cut(s) 1637
BstH2I RGCGCY 2 cut(s) 107, 123
BstHHI GCGC 3 cut(s) 100, 106, 122
BstKTI GATC 8 cut(s) 190, 367, 841, 998, 1045, 1444, 1501, 1611
BstMAI GTCTC 2 cut(s) 182, 1077
BstMBI GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1608
BstMWI GCNNNNNNNGC 8 cut(s) 23, 617, 647, 668, 938, 1238, 1343, 1634
BstNI CCWGG 4 cut(s) 1007, 1236, 1395, 1550
BstNSI RCATGY 3 cut(s) 493, 635, 1179
BstSCI CCNGG 4 cut(s) 1005, 1234, 1393, 1548
BstSFI CTRYAG 4 cut(s) 12, 528, 1146, 1459
BstSLI GKGCMC 3 cut(s) 791, 1243, 1394
BstUI CGCG 1 cut(s) 1637
BstV1I GCAGC 7 cut(s) 46, 462, 514, 598, 964, 1132, 1388
BstV2I GAAGAC 2 cut(s) 75, 132
BstX2I RGATCY 3 cut(s) 995, 1498, 1608
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 3 cut(s) 995, 1498, 1608
BsuRI GGCC 4 cut(s) 161, 941, 1320, 1392
BtgI CCRYGG 1 cut(s) 1635
BtsCI GGATG 2 cut(s) 53, 1564
BtsIMutI CAGTG 4 cut(s) 322, 910, 1032, 1314
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 7 cut(s) 69, 102, 108, 673, 677, 1177, 1479
CfoI GCGC 3 cut(s) 100, 106, 122
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
Cfr42I CCGCGG 1 cut(s) 1638
CseI GACGC 1 cut(s) 1534
CsiI ACCWGGT 1 cut(s) 1234
Csp6I GTAC 4 cut(s) 1206, 1211, 1258, 1483
CviAII CATG 8 cut(s) 332, 490, 632, 892, 1076, 1166, 1176, 1330
CviQI GTAC 4 cut(s) 1206, 1211, 1258, 1483
DdeI CTNAG 4 cut(s) 201, 615, 753, 1281
DpnI GATC 8 cut(s) 189, 366, 840, 997, 1044, 1443, 1500, 1610
DpnII GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1608
DraIII CACNNNGTG 1 cut(s) 1238
EaeI YGGCCR 1 cut(s) 1318
Eam1104I CTCTTC 3 cut(s) 950, 1098, 1614
EarI CTCTTC 3 cut(s) 950, 1098, 1614
EciI GGCGGA 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 67
Eco24I GRGCYC 4 cut(s) 11, 69, 1394, 1455
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 2 cut(s) 218, 355
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 833, 1581
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 4 cut(s) 355, 940, 1390, 1391
EcoRII CCWGG 4 cut(s) 1005, 1234, 1393, 1548
EcoRV GATATC 1 cut(s) 766
EcoT22I ATGCAT 1 cut(s) 337
EcoT38I GRGCYC 4 cut(s) 11, 69, 1394, 1455
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 8 cut(s) 335, 493, 635, 895, 1079, 1169, 1179, 1333
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 2 cut(s) 1453, 1615
FatI CATG 8 cut(s) 331, 489, 631, 891, 1075, 1165, 1175, 1329
FauI CCCGC 1 cut(s) 1630
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 7 cut(s) 35, 476, 528, 612, 953, 1146, 1377
FokI GGATG 2 cut(s) 40, 1571
FriOI GRGCYC 4 cut(s) 11, 69, 1394, 1455
Fsp4HI GCNGC 7 cut(s) 35, 476, 528, 612, 953, 1146, 1377
FspBI CTAG 1 cut(s) 1158
GlaI GCGC 3 cut(s) 99, 105, 121
GluI GCNGC 7 cut(s) 35, 476, 528, 612, 953, 1146, 1377
HaeII RGCGCY 2 cut(s) 107, 123
HaeIII GGCC 4 cut(s) 161, 941, 1320, 1392
HapII CCGG 2 cut(s) 101, 221
HgaI GACGC 1 cut(s) 1534
HhaI GCGC 3 cut(s) 100, 106, 122
Hin1II CATG 8 cut(s) 335, 493, 635, 895, 1079, 1169, 1179, 1333
Hin6I GCGC 3 cut(s) 98, 104, 120
HinP1I GCGC 3 cut(s) 98, 104, 120
HinfI GANTC 5 cut(s) 164, 285, 886, 1323, 1562
HpaII CCGG 2 cut(s) 101, 221
HphI GGTGA 1 cut(s) 1581
Hpy166II GTNNAC 5 cut(s) 313, 1140, 1241, 1436, 1485
Hpy188I TCNGA 5 cut(s) 64, 253, 655, 778, 1567
Hpy8I GTNNAC 5 cut(s) 313, 1140, 1241, 1436, 1485
Hpy99I CGWCG 2 cut(s) 79, 82
HpyAV CCTTC 5 cut(s) 239, 334, 346, 876, 1074
HpyCH4III ACNGT 4 cut(s) 317, 718, 1137, 1210
HpyCH4IV ACGT 2 cut(s) 1267, 1653
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 617, 647, 668, 938, 1238, 1343, 1634
HpyF3I CTNAG 4 cut(s) 201, 615, 753, 1281
HpySE526I ACGT 2 cut(s) 1267, 1653
Hsp92II CATG 8 cut(s) 335, 493, 635, 895, 1079, 1169, 1179, 1333
HspAI GCGC 3 cut(s) 98, 104, 120
KpnI GGTACC 1 cut(s) 1261
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
Ksp22I TGATCA 1 cut(s) 364
KspI CCGCGG 1 cut(s) 1638
Kzo9I GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1608
LmnI GCTCC 3 cut(s) 320, 778, 1066
Lsp1109I GCAGC 7 cut(s) 46, 462, 514, 598, 964, 1132, 1388
LweI GCATC 4 cut(s) 62, 487, 659, 1024
MabI ACCWGGT 1 cut(s) 1234
MaeI CTAG 1 cut(s) 1158
MaeII ACGT 2 cut(s) 1267, 1653
MaeIII GTNAC 3 cut(s) 286, 492, 1352
MalI GATC 8 cut(s) 189, 366, 840, 997, 1044, 1443, 1500, 1610
MboI GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1608
MfeI CAATTG 2 cut(s) 470, 1488
MflI RGATCY 3 cut(s) 995, 1498, 1608
MhlI GDGCHC 6 cut(s) 11, 69, 791, 1243, 1394, 1455
MlyI GAGTC 3 cut(s) 173, 294, 1317
MnlI CCTC 9 cut(s) 70, 151, 301, 501, 730, 733, 953, 1099, 1403
Mph1103I ATGCAT 1 cut(s) 337
MreI CGCCGGCG 1 cut(s) 100
MroNI GCCGGC 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 1572
MseI TTAA 4 cut(s) 300, 555, 797, 801
MslI CAYNNNNRTG 3 cut(s) 462, 636, 1160
MspA1I CMGCKG 1 cut(s) 1637
MspI CCGG 2 cut(s) 101, 221
MspR9I CCNGG 4 cut(s) 1007, 1236, 1395, 1550
MunI CAATTG 2 cut(s) 470, 1488
Mva1269I GAATGC 2 cut(s) 816, 1001
MvaI CCWGG 4 cut(s) 1007, 1236, 1395, 1550
MvnI CGCG 1 cut(s) 1637
MwoI GCNNNNNNNGC 8 cut(s) 23, 617, 647, 668, 938, 1238, 1343, 1634
NaeI GCCGGC 1 cut(s) 102
NdeII GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1608
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 8 cut(s) 335, 493, 635, 895, 1079, 1169, 1179, 1333
NmeAIII GCCGAG 2 cut(s) 187, 1426
NmuCI GTSAC 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 3 cut(s) 493, 635, 1179
OliI CACNNNNGTG 1 cut(s) 1160
PacI TTAATTAA 1 cut(s) 801
PaeI GCATGC 1 cut(s) 1179
PaqCI CACCTGC 1 cut(s) 28
PciI ACATGT 2 cut(s) 489, 631
PctI GAATGC 2 cut(s) 816, 1001
PdiI GCCGGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 1572
PfeI GAWTC 2 cut(s) 886, 1562
PflMI CCANNNNNTGG 3 cut(s) 992, 1254, 1555
PkrI GCNGC 7 cut(s) 36, 477, 529, 613, 954, 1147, 1378
PleI GAGTC 3 cut(s) 172, 293, 1317
PpsI GAGTC 3 cut(s) 172, 293, 1317
PpuMI RGGWCCY 1 cut(s) 355
PscI ACATGT 2 cut(s) 489, 631
PshBI ATTAAT 1 cut(s) 801
Psp124BI GAGCTC 1 cut(s) 69
Psp1406I AACGTT 1 cut(s) 1653
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 4 cut(s) 1005, 1234, 1393, 1548
PspGI CCWGG 4 cut(s) 1005, 1234, 1393, 1548
PspOMI GGGCCC 1 cut(s) 1390
PspPI GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
PspPPI RGGWCCY 1 cut(s) 355
PstI CTGCAG 2 cut(s) 532, 1150
PsuI RGATCY 3 cut(s) 995, 1498, 1608
RsaI GTAC 4 cut(s) 1207, 1212, 1259, 1484
RsaNI GTAC 4 cut(s) 1206, 1211, 1258, 1483
RseI CAYNNNNRTG 3 cut(s) 462, 636, 1160
SacI GAGCTC 1 cut(s) 69
SacII CCGCGG 1 cut(s) 1638
SaqAI TTAA 4 cut(s) 300, 555, 797, 801
SatI GCNGC 7 cut(s) 35, 476, 528, 612, 953, 1146, 1377
Sau3AI GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1608
Sau96I GGNCC 5 cut(s) 218, 355, 940, 1390, 1391
SchI GAGTC 3 cut(s) 173, 294, 1317
ScrFI CCNGG 4 cut(s) 1007, 1236, 1395, 1550
SduI GDGCHC 6 cut(s) 11, 69, 791, 1243, 1394, 1455
SexAI ACCWGGT 1 cut(s) 1234
SfaNI GCATC 4 cut(s) 62, 487, 659, 1024
SfcI CTRYAG 4 cut(s) 12, 528, 1146, 1459
Sfr303I CCGCGG 1 cut(s) 1638
SgrAI CRCCGGYG 1 cut(s) 100
SgrBI CCGCGG 1 cut(s) 1638
SinI GGWCC 2 cut(s) 218, 355
SmiMI CAYNNNNRTG 3 cut(s) 462, 636, 1160
SphI GCATGC 1 cut(s) 1179
SsiI CCGC 7 cut(s) 57, 1130, 1230, 1475, 1502, 1635, 1637
SspI AATATT 1 cut(s) 914
SspMI CTAG 1 cut(s) 1158
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 4 cut(s) 1005, 1234, 1393, 1548
TaaI ACNGT 4 cut(s) 317, 718, 1137, 1210
TaiI ACGT 2 cut(s) 1270, 1656
TaqI TCGA 3 cut(s) 186, 735, 1185
TfiI GAWTC 2 cut(s) 886, 1562
Tru1I TTAA 4 cut(s) 300, 555, 797, 801
Tru9I TTAA 4 cut(s) 300, 555, 797, 801
TscAI CASTG 4 cut(s) 322, 910, 1032, 1321
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 7 cut(s) 34, 475, 527, 611, 952, 1145, 1376
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 5 cut(s) 284, 819, 924, 1131, 1532
TspGWI ACGGA 3 cut(s) 144, 157, 257
TspRI CASTG 4 cut(s) 322, 910, 1032, 1321
Van91I CCANNNNNTGG 3 cut(s) 992, 1254, 1555
VneI GTGCAC 1 cut(s) 1239
VpaK11BI GGWCC 2 cut(s) 218, 355
VspI ATTAAT 1 cut(s) 801
XapI RAATTY 1 cut(s) 1568
XceI RCATGY 3 cut(s) 493, 635, 1179
XcmI CCANNNNNNNNNTGG 1 cut(s) 1251
XmnI GAANNNNTTC 1 cut(s) 1572
XspI CTAG 1 cut(s) 1158
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.