Rw4G020610
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010022
Physical Location & Seq
Forward (+)
44617712 .. 44621291
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020610.1

Sequence Viewer

Length: 933 bp
ATGATAGTCGTATATTCGTTAATTAATGAGATTCACGGACGATGCGATTCCCGTATTGTCAATTTGGGCCAGACAGAAGCTAAACTGTACAAGTTGGGCCCCTTGCAGTCTGTCTGCATATCTGAAGGTTCTGATACCGGCAAAGAGAAATTGTATTCAAGGGGCATCACAATCCAATTTAAGAATGAGGAGGAGAGTGGAGCCTTCCATCATGCATTTGAGCAATGGAAGACGGAGCTTGTTGTTGAAGGAAAATGTTTACCAAATGGAGAGCTAACATCTTCCAAAAGCAAGTTTGATGACAAAATAGAGCCATCTTCTGCCAAAATGTACTTCCATTACTATGGACAATTGCTACATCAACAAAACATGTTACAAGATTATGTGAGGACAGGAACCTATTATGCAGCAGTGATTGAAAACCGTGCAGATTTCGCTGGCCGTGTGGTAGTTGATGTTGGTGCCGGTAGTGGTATTCTGTCATTATTTGCTGCTCAGGCTGGTGCAAAGCATGTTTATGCTGTGGAAGCATCTGAAATGGCAGAATATGCACGCAAACTAATTGCTGGGAACCCTTCACTGGGTCAAAGGATAACTGTAATCAAAGGTAAAGTTGAGGAGGTTGAATTACCTGAGAAAGCAGATATACTTATCTCGGAGCCAATGGGCACATTGTTAATTAATGAAAGAATGCTGGAGACCTATGTGATTGCAAGAGATCGGTTTCTTCAGCCAAATGGAAAAATGTTTCCTGGAGTTGGAAGGATACACATGGCACCTTTCAGTGATGAATATTTGTTTGTTGAAATTGCAAATAAGGCTCTCTTCTGGCAGCAACAAAATTATTATGGTGTTGATTTGACCCCCTTGTATGGATCTGCATTCGAAGGATATTTTTCACAGTTGTTGATCTTTCTATACTATGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008469 GO:0008757 GO:0009628 GO:0009651 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009987 GO:0010035 GO:0010038 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016273 GO:0016274 GO:0016277 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019919 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032259 GO:0032501 GO:0032502 GO:0034969 GO:0034970 GO:0034971 GO:0034972 GO:0035241 GO:0035242 GO:0035246 GO:0035247 GO:0036211 GO:0042054 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046686 GO:0046982 GO:0046983 GO:0048518 GO:0048522 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0140096 GO:1901564 GO:1902680 GO:1902882 GO:1902884 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01522 map01522
Pfam Domains
Protein Families
Methyltransf_25 PrmA

Protein Analysis

310

Amino Acids

34.88

Weight (kDa)

5.48

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_PRMT_N PF25350 17 - 77 3.5e-25 Protein arginine methyltransferase N-terminal PH domain
PrmA PF06325 146 - 220 6.2e-08 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_25 PF13649 150 - 247 1.4e-07 Methyltransferase domain
Methyltransf_11 PF08241 151 - 249 1e-05 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 461, 775
AclWI GGATC 1 cut(s) 883
AcoI YGGCCR 1 cut(s) 439
AcuI CTGAAG 2 cut(s) 144, 713
AfaI GTAC 2 cut(s) 89, 332
AfiI CCNNNNNNNGG 4 cut(s) 580, 581, 758, 872
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 5 cut(s) 159, 248, 419, 626, 806
AjnI CCWGG 1 cut(s) 751
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 3 cut(s) 80, 238, 274
AluI AGCT 3 cut(s) 80, 238, 274
Alw26I GTCTC 1 cut(s) 692
AlwI GGATC 1 cut(s) 883
AoxI GGCC 3 cut(s) 67, 97, 439
ApaI GGGCCC 1 cut(s) 101
ApeKI GCWGC 3 cut(s) 407, 491, 832
AseI ATTAAT 2 cut(s) 24, 681
AspS9I GGNCC 3 cut(s) 67, 97, 98
AsuII TTCGAA 1 cut(s) 885
BaeGI GKGCMC 2 cut(s) 101, 671
BanI GGYRCC 2 cut(s) 461, 775
BanII GRGCYC 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 236
BbvI GCAGC 3 cut(s) 419, 478, 844
BccI CCATC 2 cut(s) 216, 322
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 1 cut(s) 753
BciVI GTATCC 1 cut(s) 759
BcoDI GTCTC 1 cut(s) 692
BfuI GTATCC 1 cut(s) 759
BisI GCNGC 3 cut(s) 408, 492, 833
BlsI GCNGC 3 cut(s) 409, 493, 834
Bme1390I CCNGG 1 cut(s) 753
BmgT120I GGNCC 3 cut(s) 67, 97, 98
BmiI GGNNCC 8 cut(s) 99, 100, 202, 397, 463, 572, 660, 777
BmrFI CCNGG 1 cut(s) 753
BmrI ACTGGG 1 cut(s) 590
BmsI GCATC 3 cut(s) 32, 174, 539
BmuI ACTGGG 1 cut(s) 590
BpiI GAAGAC 1 cut(s) 236
BpmI CTGGAG 2 cut(s) 716, 774
Bpu10I CCTNAGC 1 cut(s) 495
Bpu14I TTCGAA 1 cut(s) 885
BsaI GGTCTC 1 cut(s) 692
Bsc4I CCNNNNNNNGG 4 cut(s) 580, 581, 758, 872
Bse118I RCCGGY 2 cut(s) 137, 464
Bse1I ACTGG 1 cut(s) 585
Bse3DI GCAATG 1 cut(s) 230
BseBI CCWGG 1 cut(s) 753
BseLI CCNNNNNNNGG 4 cut(s) 580, 581, 758, 872
BseMI GCAATG 1 cut(s) 230
BseMII CTCAG 2 cut(s) 509, 624
BseNI ACTGG 1 cut(s) 585
BseRI GAGGAG 3 cut(s) 203, 206, 632
BseSI GKGCMC 2 cut(s) 101, 671
BseXI GCAGC 3 cut(s) 419, 478, 844
BseYI CCCAGC 1 cut(s) 566
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 3 cut(s) 69, 99, 441
BshNI GGYRCC 2 cut(s) 461, 775
BsiSI CCGG 2 cut(s) 138, 465
BslI CCNNNNNNNGG 4 cut(s) 580, 581, 758, 872
BsmAI GTCTC 1 cut(s) 692
BsmI GAATGC 2 cut(s) 696, 881
BsnI GGCC 3 cut(s) 69, 99, 441
Bso31I GGTCTC 1 cut(s) 692
Bsp119I TTCGAA 1 cut(s) 885
Bsp120I GGGCCC 1 cut(s) 97
Bsp1286I GDGCHC 2 cut(s) 101, 671
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 3 cut(s) 718, 875, 909
BspANI GGCC 3 cut(s) 69, 99, 441
BspCNI CTCAG 2 cut(s) 508, 625
BspLI GGNNCC 8 cut(s) 99, 100, 202, 397, 463, 572, 660, 777
BspPI GGATC 1 cut(s) 883
BspT104I TTCGAA 1 cut(s) 885
BspT107I GGYRCC 2 cut(s) 461, 775
BspTNI GGTCTC 1 cut(s) 692
BsrDI GCAATG 1 cut(s) 230
BsrFI RCCGGY 2 cut(s) 137, 464
BsrGI TGTACA 1 cut(s) 87
BsrI ACTGG 1 cut(s) 585
BssAI RCCGGY 2 cut(s) 137, 464
BssMI GATC 3 cut(s) 718, 875, 909
Bst2UI CCWGG 1 cut(s) 753
Bst4CI ACNGT 4 cut(s) 87, 425, 598, 903
Bst6I CTCTTC 1 cut(s) 830
BstAPI GCANNNNNTGC 1 cut(s) 548
BstAUI TGTACA 1 cut(s) 87
BstBI TTCGAA 1 cut(s) 885
BstC8I GCNNGC 2 cut(s) 439, 553
BstDEI CTNAG 2 cut(s) 495, 633
BstKTI GATC 3 cut(s) 721, 878, 912
BstMAI GTCTC 1 cut(s) 692
BstMBI GATC 3 cut(s) 718, 875, 909
BstMWI GCNNNNNNNGC 5 cut(s) 434, 497, 527, 548, 818
BstNI CCWGG 1 cut(s) 753
BstNSI RCATGY 2 cut(s) 373, 515
BstSCI CCNGG 1 cut(s) 751
BstSLI GKGCMC 2 cut(s) 101, 671
BstV1I GCAGC 3 cut(s) 419, 478, 844
BstV2I GAAGAC 1 cut(s) 236
BstX2I RGATCY 1 cut(s) 875
BstXI CCANNNNNNTGG 1 cut(s) 344
BstYI RGATCY 1 cut(s) 875
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 3 cut(s) 69, 99, 441
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 3 cut(s) 417, 578, 790
Cac8I GCNNGC 2 cut(s) 439, 553
Cfr10I RCCGGY 2 cut(s) 137, 464
Cfr13I GGNCC 3 cut(s) 67, 97, 98
Csp6I GTAC 2 cut(s) 88, 331
CviAII CATG 4 cut(s) 212, 370, 512, 772
CviQI GTAC 2 cut(s) 88, 331
DdeI CTNAG 2 cut(s) 495, 633
DpnI GATC 3 cut(s) 720, 877, 911
DpnII GATC 3 cut(s) 718, 875, 909
EaeI YGGCCR 1 cut(s) 439
Eam1104I CTCTTC 1 cut(s) 830
EarI CTCTTC 1 cut(s) 830
Eco24I GRGCYC 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 692
Eco57I CTGAAG 2 cut(s) 144, 713
EcoO109I RGGNCCY 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 751
EcoT22I ATGCAT 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 101
FaeI CATG 4 cut(s) 215, 373, 515, 775
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FatI CATG 4 cut(s) 211, 369, 511, 771
Fnu4HI GCNGC 3 cut(s) 408, 492, 833
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 3 cut(s) 408, 492, 833
GluI GCNGC 3 cut(s) 408, 492, 833
GsaI CCCAGC 1 cut(s) 570
GsuI CTGGAG 2 cut(s) 716, 774
HaeIII GGCC 3 cut(s) 69, 99, 441
HapII CCGG 2 cut(s) 138, 465
Hin1II CATG 4 cut(s) 215, 373, 515, 775
HinfI GANTC 2 cut(s) 31, 47
HpaII CCGG 2 cut(s) 138, 465
Hpy166II GTNNAC 1 cut(s) 260
Hpy188I TCNGA 4 cut(s) 124, 133, 535, 658
Hpy8I GTNNAC 1 cut(s) 260
HpyAV CCTTC 6 cut(s) 119, 214, 242, 585, 756, 881
HpyCH4III ACNGT 4 cut(s) 87, 425, 598, 903
HpyF10VI GCNNNNNNNGC 5 cut(s) 434, 497, 527, 548, 818
HpyF3I CTNAG 2 cut(s) 495, 633
Hsp92II CATG 4 cut(s) 215, 373, 515, 775
Kzo9I GATC 3 cut(s) 718, 875, 909
LmnI GCTCC 3 cut(s) 200, 235, 658
Lsp1109I GCAGC 3 cut(s) 419, 478, 844
LweI GCATC 3 cut(s) 32, 174, 539
MaeIII GTNAC 1 cut(s) 372
MalI GATC 3 cut(s) 720, 877, 911
MboI GATC 3 cut(s) 718, 875, 909
MboII GAAGA 5 cut(s) 241, 273, 309, 719, 817
MfeI CAATTG 1 cut(s) 350
MflI RGATCY 1 cut(s) 875
MhlI GDGCHC 2 cut(s) 101, 671
MmeI TCCRAC 1 cut(s) 739
MnlI CCTC 5 cut(s) 181, 184, 381, 610, 613
Mph1103I ATGCAT 1 cut(s) 217
MseI TTAA 5 cut(s) 20, 24, 180, 677, 681
MslI CAYNNNNRTG 2 cut(s) 342, 516
MspI CCGG 2 cut(s) 138, 465
MspR9I CCNGG 1 cut(s) 753
MunI CAATTG 1 cut(s) 350
Mva1269I GAATGC 2 cut(s) 696, 881
MvaI CCWGG 1 cut(s) 753
MwoI GCNNNNNNNGC 5 cut(s) 434, 497, 527, 548, 818
NdeII GATC 3 cut(s) 718, 875, 909
NlaIII CATG 4 cut(s) 215, 373, 515, 775
NlaIV GGNNCC 8 cut(s) 99, 100, 202, 397, 463, 572, 660, 777
NsiI ATGCAT 1 cut(s) 217
NspI RCATGY 2 cut(s) 373, 515
NspV TTCGAA 1 cut(s) 885
PacI TTAATTAA 2 cut(s) 24, 681
PciI ACATGT 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PctI GAATGC 2 cut(s) 696, 881
PfeI GAWTC 2 cut(s) 31, 47
PfoI TCCNGGA 1 cut(s) 751
PkrI GCNGC 3 cut(s) 409, 493, 834
PscI ACATGT 1 cut(s) 369
PshBI ATTAAT 2 cut(s) 24, 681
Psp6I CCWGG 1 cut(s) 751
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 1 cut(s) 751
PspN4I GGNNCC 8 cut(s) 99, 100, 202, 397, 463, 572, 660, 777
PspOMI GGGCCC 1 cut(s) 97
PspPI GGNCC 3 cut(s) 67, 97, 98
PsuI RGATCY 1 cut(s) 875
RsaI GTAC 2 cut(s) 89, 332
RsaNI GTAC 2 cut(s) 88, 331
RseI CAYNNNNRTG 2 cut(s) 342, 516
SaqAI TTAA 5 cut(s) 20, 24, 180, 677, 681
SatI GCNGC 3 cut(s) 408, 492, 833
Sau3AI GATC 3 cut(s) 718, 875, 909
Sau96I GGNCC 3 cut(s) 67, 97, 98
ScrFI CCNGG 1 cut(s) 753
SduI GDGCHC 2 cut(s) 101, 671
SfaNI GCATC 3 cut(s) 32, 174, 539
SfuI TTCGAA 1 cut(s) 885
SmiMI CAYNNNNRTG 2 cut(s) 342, 516
SspI AATATT 1 cut(s) 794
StyD4I CCNGG 1 cut(s) 751
TaaI ACNGT 4 cut(s) 87, 425, 598, 903
TaqI TCGA 1 cut(s) 885
TatI WGTACW 2 cut(s) 87, 330
TfiI GAWTC 2 cut(s) 31, 47
Tru1I TTAA 5 cut(s) 20, 24, 180, 677, 681
Tru9I TTAA 5 cut(s) 20, 24, 180, 677, 681
TscAI CASTG 3 cut(s) 417, 585, 790
TseI GCWGC 3 cut(s) 407, 491, 832
TspDTI ATGAA 2 cut(s) 699, 804
TspGWI ACGGA 2 cut(s) 51, 248
TspRI CASTG 3 cut(s) 417, 585, 790
VspI ATTAAT 2 cut(s) 24, 681
XceI RCATGY 2 cut(s) 373, 515
Zsp2I ATGCAT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.