MD13G1176100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013404
Physical Location & Seq
Forward (+)
14558285 .. 14559230
946 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1176100.v1.1.491

Sequence Viewer

Length: 291 bp
ATGGCGTCGCCCGCCGCAAGGATCACCTTCCGCATAGTATTCGTGGCTCTGGTCGTTCTCGTCCTCTTCTACGTTGGCCGCCCTCTCTACTGGAAAATCTCCGCCACCATCCACGAGATCCGCCAAAACAAACAGACCGTTAAGCAAGGTATATCGCAGATTGTTCTGGAAGCTCAGAAATCGGTCGGTTGGTACCACGACGAGTCGGATTCGGGTGCCCGCTACGATAGGGCCGCGAAGAATGTCGGGTTGGCCACGACCCGGAGGCTGCTGCTCCGCCAGGTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

10.87

Weight (kDa)

10.47

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18850
fragaria_vesca FvH4_4g16860
malus_domestica MD13G1176100.v1.1 MD16G1177900.v1.1
prunus_persica Prupe.1G145200_v2.0.a1
pyrus_communis pycom13g15180 pycom16g14930
rosa_chinensis RchiOBHm_Chr4g0420431
rosa_multiflora Rmu_co8133642.1_g000001 Rmu_sc0002248.1_g000025 Rmu_sc0033516.1_g000002
rosa_roxburghii Rroxscaffold_5G00363070
rosa_rugosa Rorug04G0163800
rosa_samantha Rh4AG225300 Rh4BG228100 Rh4CG239600 Rh4DG223800
rosa_wichuraiana Rw4G019450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 192
AccB1I GGYRCC 2 cut(s) 192, 215
AccII CGCG 1 cut(s) 236
AciI CCGC 9 cut(s) 12, 15, 31, 79, 102, 121, 220, 234, 277
AclWI GGATC 2 cut(s) 29, 112
AcoI YGGCCR 2 cut(s) 76, 252
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 18, 261
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 1 cut(s) 173
AluI AGCT 1 cut(s) 173
AlwI GGATC 2 cut(s) 29, 112
AoxI GGCC 3 cut(s) 76, 231, 252
ApeKI GCWGC 2 cut(s) 268, 271
Asp718I GGTACC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 231
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 254
BanI GGYRCC 2 cut(s) 192, 215
BauI CACGAG 1 cut(s) 113
BbvI GCAGC 2 cut(s) 255, 258
BccI CCATC 1 cut(s) 116
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 1 cut(s) 281
BcnI CCSGG 1 cut(s) 262
BisI GCNGC 5 cut(s) 15, 79, 234, 269, 272
BlsI GCNGC 5 cut(s) 16, 80, 235, 270, 273
Bme1390I CCNGG 2 cut(s) 262, 281
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 2 cut(s) 194, 217
BmrFI CCNGG 2 cut(s) 262, 281
BpuMI CCSGG 1 cut(s) 262
BsaHI GRCGYC 1 cut(s) 5
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 261
Bse1I ACTGG 1 cut(s) 95
BseBI CCWGG 1 cut(s) 281
BseGI GGATG 1 cut(s) 108
BseLI CCNNNNNNNGG 2 cut(s) 18, 261
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 2 cut(s) 255, 258
Bsh1236I CGCG 1 cut(s) 236
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 3 cut(s) 78, 233, 254
BshNI GGYRCC 2 cut(s) 192, 215
BsiEI CGRYCG 1 cut(s) 186
BsiSI CCGG 1 cut(s) 262
BslI CCNNNNNNNGG 2 cut(s) 18, 261
BsnI GGCC 3 cut(s) 78, 233, 254
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 2 cut(s) 21, 117
BspACI CCGC 9 cut(s) 12, 15, 31, 79, 102, 121, 220, 234, 277
BspANI GGCC 3 cut(s) 78, 233, 254
BspCNI CTCAG 1 cut(s) 187
BspFNI CGCG 1 cut(s) 236
BspLI GGNNCC 2 cut(s) 194, 217
BspPI GGATC 2 cut(s) 29, 112
BspT107I GGYRCC 2 cut(s) 192, 215
BsrI ACTGG 1 cut(s) 95
BssMI GATC 2 cut(s) 21, 117
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 113
Bst2BI CACGAG 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 12, 220
BstDEI CTNAG 1 cut(s) 174
BstF5I GGATG 1 cut(s) 108
BstFNI CGCG 1 cut(s) 236
BstKTI GATC 2 cut(s) 24, 120
BstMBI GATC 2 cut(s) 21, 117
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 260, 279
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 2 cut(s) 255, 258
BstX2I RGATCY 1 cut(s) 117
BstYI RGATCY 1 cut(s) 117
BsuRI GGCC 3 cut(s) 78, 233, 254
BtsCI GGATG 1 cut(s) 108
Cac8I GCNNGC 2 cut(s) 12, 220
Cfr13I GGNCC 1 cut(s) 231
Csp6I GTAC 1 cut(s) 193
CviJI RGCY 6 cut(s) 47, 78, 173, 233, 254, 268
CviKI_1 RGCY 6 cut(s) 47, 78, 173, 233, 254, 268
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 174
DpnI GATC 2 cut(s) 23, 119
DpnII GATC 2 cut(s) 21, 117
EaeI YGGCCR 2 cut(s) 76, 252
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
EciI GGCGGA 3 cut(s) 91, 110, 266
EcoRII CCWGG 1 cut(s) 279
FaiI YATR 2 cut(s) 35, 152
FauI CCCGC 2 cut(s) 19, 227
Fnu4HI GCNGC 5 cut(s) 15, 79, 234, 269, 272
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 5 cut(s) 15, 79, 234, 269, 272
GluI GCNGC 5 cut(s) 15, 79, 234, 269, 272
HaeIII GGCC 3 cut(s) 78, 233, 254
HapII CCGG 1 cut(s) 262
Hin1I GRCGYC 1 cut(s) 5
HinfI GANTC 2 cut(s) 203, 209
HpaII CCGG 1 cut(s) 262
HphI GGTGA 1 cut(s) 16
Hpy188I TCNGA 2 cut(s) 177, 208
Hpy188III TCNNGA 2 cut(s) 167, 288
Hpy99I CGWCG 2 cut(s) 10, 203
HpyAV CCTTC 1 cut(s) 37
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4IV ACGT 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 5
KpnI GGTACC 1 cut(s) 196
Kzo9I GATC 2 cut(s) 21, 117
LmnI GCTCC 1 cut(s) 279
LpnPI CCDG 5 cut(s) 35, 76, 152, 266, 275
Lsp1109I GCAGC 2 cut(s) 255, 258
MaeII ACGT 1 cut(s) 72
MalI GATC 2 cut(s) 23, 119
MboI GATC 2 cut(s) 21, 117
MboII GAAGA 2 cut(s) 58, 250
MflI RGATCY 1 cut(s) 117
MhlI GDGCHC 1 cut(s) 220
MlsI TGGCCA 1 cut(s) 254
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 186
MnlI CCTC 3 cut(s) 74, 93, 258
Mox20I TGGCCA 1 cut(s) 254
MscI TGGCCA 1 cut(s) 254
MseI TTAA 1 cut(s) 141
Msp20I TGGCCA 1 cut(s) 254
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 2 cut(s) 262, 281
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 262
NdeII GATC 2 cut(s) 21, 117
NlaIV GGNNCC 2 cut(s) 194, 217
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 5 cut(s) 16, 80, 235, 270, 273
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 2 cut(s) 194, 217
PspPI GGNCC 1 cut(s) 231
PsuI RGATCY 1 cut(s) 117
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 5 cut(s) 15, 79, 234, 269, 272
Sau3AI GATC 2 cut(s) 21, 117
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 262, 281
SduI GDGCHC 1 cut(s) 220
SetI ASST 5 cut(s) 29, 75, 151, 175, 285
SsiI CCGC 9 cut(s) 12, 15, 31, 79, 102, 121, 220, 234, 277
StyD4I CCNGG 2 cut(s) 260, 279
TaaI ACNGT 1 cut(s) 139
TaiI ACGT 1 cut(s) 75
TaqII GACCGA 1 cut(s) 172
TauI GCSGC 3 cut(s) 17, 81, 236
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TseI GCWGC 2 cut(s) 268, 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.