Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008688

Physical Location & Seq

Genomic Coordinates Forward (+)

21365642 .. 21372618

Gene Structure 6977 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1220800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGAACTGCGCACTATCCGGCGAGGTGCCGGAGGAGCCTGTGGTCTCCAAGAAGTCCGGGTTGCTCTTCGAGAAGCGTTTAATCGAACGACATATCTCGGAATATGGAAAATGCCCGGTAACCGGTGAACCACTTACCGCTGATGATATCATTCCAGTTCAAACGGGGAAGATAGTGAGGCCCAGACCTGCACAAGCTGCTAGTATTCCTGGAATGCTTGGAATGTTCCAGCTTGAATGGGATAGTTTGATGCTAGCCAACTTTGCAATGGAGAAACAACTACATACAGCAAGGCAAGAGTTGAGTCATGCTTTGTATCAGCATGATGCCGCTTGCCGTGTTATTGCAAGACTCAAAAAAGAAAGGGATGAAGCAAGGTCATTACTTGCCCAGGCTGAGAGACAAATTCCCGCACAATCAACAGTTACGGCAAATGCTGCTGTCCATAGCAATGGAACACCAGTTGCTGATGACCAGCAAGGACCTGGTGCGAGCAATATACGACCTGGAATATCTGCCAGTATCCTAGAGAAGATGACCGAATGTAATGTAACTCTTTCACAGCAGCGGAAGATGCGGCAGATATCCCCAACTTTGGCTCCAGTTGATGCGTTGGAGAGGTATACTCAGATATCTAGTCATCCTCTTCATAGAACAAGCAAACCAGGAATTTTATCAATTGACATTCAATCTAACAAGAACCTCATTGCAACTGGAGGGGTTGATACAAATGCTGTAATCTTTGATCAAACATCAGCGCAAATCATATCTACTCTTAGTGGTCATTCGAAGAAGGTTACAAGCGTGAAATTTGTAGGTCAGGATGATCTGTTCATAAGTGGGTCAGCAGACAAGACAGTTCGCATATGGCAAGGCACTGATGATGGGAACTATAACTGTAGGCACATTTTGAAGGACCATACTGCGGAGGTGCAAGCTGTCACTGTACATGCCACGGGTGACTACTTTGTGACTGCTTCTCTTGATAGTACATGGTGCTTTTACGATATTGCATCTGGGTTATGTCTAACACAGGTTGAGGACGCTTCTGCTTCGGAAGGCGGCTATACATCTGTGGCATTTCATCCTGATGGTCTCATTCTTGGAACAGGCACCTCAGAGGCTCTTGTGAAAATTTGGGATGTAAAGGGTCAGTCAAATGTTGCAACATTTGAGGGACATGTTGGCGCAATAACTGCAATCTCCTTCTCTGAGAATGGTTACTTTCTTGCGACCGCAGCTAGTGATGGCATTAAGTTGTGGGATCTACGCAAATTGAGGAACTTCAAGAAGTTTACTCCATACGGTGCTGAACCAACAAACTGTGTTGAATTTGACTACAGTGGAAATTACCTTGCTGCTGCAGGCTCTGATATAAGAATCATCCAAACTGGTAGCGTCAAAAGTGAATGGAACACTGTAAAGACTTTCCCTGATATGTCTGGCACAGGTAAAACAACAAGCGTGAAGTTTGGACGAGATGCAAATTATATTGCAGTAGGATCAATGGACCGAAACCTTCGCATTTTTGGCCTGCCTGGCGACGATAGTGCAATGGAGTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0000349 GO:0000375 GO:0000377 GO:0000393 GO:0000398 GO:0000974 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008380 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022607 GO:0022613 GO:0022618 GO:0030312 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0034622 GO:0034641 GO:0036211 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0061630 GO:0061659 GO:0065003 GO:0070013 GO:0070534 GO:0070647 GO:0071006 GO:0071012 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0080008 GO:0090304 GO:0098542 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 ko04120 map03040 map04120

Pfam Domains

Protein Families

Prp19 U-box WD40

Protein Analysis

522

Amino Acids

56.38

Weight (kDa)

5.93

Isoelectric Point (pI)

29.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Prp19	PF08606	66 - 131	6.4e-29	Prp19/Pso4-like
WD40_Prp19	PF24814	216 - 504	1.1e-105	Prp19 WD40 domain
Beta-prop_DCAF12	PF23760	218 - 296	3.4e-06	DDB1- and CUL4-associated factor 12 beta propeller
Beta-prop_WDR5	PF25175	220 - 348	7.1e-23	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	221 - 385	2.2e-09	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	223 - 337	8.4e-19	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	223 - 361	4.2e-11	G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd	PF25172	223 - 334	5.4e-14	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	223 - 345	2.5e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	224 - 296	1.7e-09	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	224 - 422	3.9e-08	Echinoderm microtubule-associated protein first beta-propeller
WD40_CDC20-Fz	PF24807	226 - 346	7.3e-11	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	229 - 421	5.9e-19	MABP1/WDR62 second WD40 domain
Beta-prop_WDR19_1st	PF23389	232 - 455	3.4e-06	WDR19 first beta-propeller
WD40	PF00400	255 - 290	9.2e-09	WD domain, G-beta repeat
EIF3I	PF24805	259 - 350	4e-08	EIF3I
Beta-prop_THOC3	PF25174	262 - 511	5.3e-38	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	298 - 424	2.9e-07	TEP-1 second beta-propeller
WDR55	PF24796	300 - 445	3.8e-12	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	305 - 456	2.5e-13	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	312 - 431	4.2e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	322 - 418	4.2e-10	WDR90/POC16, second beta-propeller
WD40_Gbeta	PF25391	324 - 510	1.1e-12	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	330 - 459	5.5e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	352 - 421	1.7e-06	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	353 - 510	3.9e-21	WDR5 beta-propeller domain
EIF3I	PF24805	356 - 509	8.6e-08	EIF3I
WD40_WDHD1_1st	PF24817	357 - 510	1.7e-13	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	357 - 459	9.1e-14	WDR3 first beta-propeller domain
WD40	PF00400	387 - 422	4.7e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008688)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04510 AT1G04510 AT2G33340 AT2G33340 AT2G33340
fragaria_vesca	FvH4_4g04520 FvH4_4g04520
malus_domestica	MD13G1220800.v1.1 MD16G1225900.v1.1
prunus_persica	Prupe.1G047500_v2.0.a1
pyrus_communis	pycom13g19530 pycom16g18900
rosa_chinensis	RchiOBHm_Chr4g0395291
rosa_laevigata	RLG00000009634
rosa_multiflora	Rmu_sc0015296.1_g000001 Rmu_sc0032966.1_g000001
rosa_roxburghii	Rroxscaffold_5G00340170
rosa_rugosa	Rorug03G0358300
rosa_samantha	Rh4AG058200 Rh4BG057000 Rh4CG062800 Rh4DG053700
rosa_wichuraiana	Rw4G004680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	10
Acc36I	ACCTGC	1 cut(s)	196
AccB1I	GGYRCC	2 cut(s)	25, 1112
AccI	GTMKAC	1 cut(s)	623
AciI	CCGC	8 cut(s)	138, 330, 411, 568, 577, 926, 1062, 1236
AclWI	GGATC	2 cut(s)	1272, 1510
AcsI	RAATTY	5 cut(s)	405, 669, 809, 1134, 1331
AfaI	GTAC	2 cut(s)	948, 991
AfiI	CCNNNNNNNGG	3 cut(s)	122, 595, 925
AflIII	ACRYGT	1 cut(s)	1180
AgeI	ACCGGT	1 cut(s)	122
AgsI	TTSAA	6 cut(s)	161, 236, 689, 913, 1288, 1331
AjnI	CCWGG	6 cut(s)	208, 390, 484, 505, 664, 1537
AloI	GAACNNNNNNTCC	2 cut(s)	815, 847
AluBI	AGCT	4 cut(s)	197, 232, 938, 1241
AluI	AGCT	4 cut(s)	197, 232, 938, 1241
Alw26I	GTCTC	3 cut(s)	49, 394, 1100
AlwI	GGATC	2 cut(s)	1272, 1510
AlwNI	CAGNNNCTG	2 cut(s)	467, 1370
AoxI	GGCC	2 cut(s)	179, 1531
ApeKI	GCWGC	6 cut(s)	197, 437, 565, 1238, 1358, 1361
ApoI	RAATTY	5 cut(s)	405, 669, 809, 1134, 1331
AsiGI	ACCGGT	1 cut(s)	122
AspLEI	GCGC	3 cut(s)	11, 760, 1190
AspS9I	GGNCC	4 cut(s)	180, 482, 916, 1510
AsuC2I	CCSGG	2 cut(s)	58, 116
AsuHPI	GGTGA	2 cut(s)	137, 971
AsuII	TTCGAA	1 cut(s)	788
AsuNHI	GCTAGC	1 cut(s)	253
AvaII	GGWCC	3 cut(s)	482, 916, 1510
BanI	GGYRCC	2 cut(s)	25, 1112
BbvI	GCAGC	6 cut(s)	184, 424, 577, 1250, 1345, 1348
BccI	CCATC	3 cut(s)	878, 1085, 1241
BceAI	ACGGC	2 cut(s)	321, 444
BcgI	CGANNNNNNTGC	3 cut(s)	1532, 1542, 1566
BciT130I	CCWGG	6 cut(s)	210, 392, 486, 507, 666, 1539
BciVI	GTATCC	1 cut(s)	533
BclI	TGATCA	1 cut(s)	745
BcnI	CCSGG	2 cut(s)	58, 116
BcoDI	GTCTC	3 cut(s)	49, 394, 1100
BfaI	CTAG	5 cut(s)	201, 254, 527, 636, 1242
BfmI	CTRYAG	3 cut(s)	898, 1339, 1362
BfuAI	ACCTGC	1 cut(s)	196
BfuI	GTATCC	1 cut(s)	533
BglI	GCCNNNNNGGC	1 cut(s)	1539
BisI	GCNGC	9 cut(s)	198, 330, 438, 566, 578, 1063, 1239, 1359, 1362
BlsI	GCNGC	9 cut(s)	199, 331, 439, 567, 579, 1064, 1240, 1360, 1363
Bme1390I	CCNGG	8 cut(s)	58, 116, 210, 392, 486, 507, 666, 1539
Bme18I	GGWCC	3 cut(s)	482, 916, 1510
BmgT120I	GGNCC	4 cut(s)	180, 482, 916, 1510
BmiI	GGNNCC	4 cut(s)	27, 36, 600, 1114
BmrFI	CCNGG	8 cut(s)	58, 116, 210, 392, 486, 507, 666, 1539
BmsI	GCATC	6 cut(s)	240, 316, 564, 598, 1022, 1471
BmtI	GCTAGC	1 cut(s)	257
BplI	GAGNNNNNCTC	2 cut(s)	610, 642
BpmI	CTGGAG	2 cut(s)	585, 735
Bpu14I	TTCGAA	1 cut(s)	788
BpuMI	CCSGG	2 cut(s)	58, 116
BsaI	GGTCTC	2 cut(s)	49, 1100
BsaJI	CCNNGG	2 cut(s)	390, 954
BsaWI	WCCGGW	1 cut(s)	122
BsaXI	ACNNNNNCTCC	2 cut(s)	583, 613
Bsc4I	CCNNNNNNNGG	3 cut(s)	122, 595, 925
Bse118I	RCCGGY	1 cut(s)	122
Bse1I	ACTGG	6 cut(s)	155, 461, 519, 602, 718, 1396
Bse3DI	GCAATG	4 cut(s)	273, 457, 705, 1560
BseBI	CCWGG	6 cut(s)	210, 392, 486, 507, 666, 1539
BseDI	CCNNGG	2 cut(s)	390, 954
BseGI	GGATG	6 cut(s)	373, 640, 829, 1084, 1147, 1383
BseLI	CCNNNNNNNGG	3 cut(s)	122, 595, 925
BseMI	GCAATG	4 cut(s)	273, 457, 705, 1560
BseMII	CTCAG	4 cut(s)	387, 641, 1131, 1203
BseNI	ACTGG	6 cut(s)	155, 461, 519, 602, 718, 1396
BseRI	GAGGAG	1 cut(s)	47
BseXI	GCAGC	6 cut(s)	184, 424, 577, 1250, 1345, 1348
BsgI	GTGCAG	1 cut(s)	174
Bsh1285I	CGRYCG	1 cut(s)	1236
BshFI	GGCC	2 cut(s)	181, 1533
BshNI	GGYRCC	2 cut(s)	25, 1112
BshTI	ACCGGT	1 cut(s)	122
BsiEI	CGRYCG	1 cut(s)	1236
BsiSI	CCGG	5 cut(s)	18, 29, 57, 116, 123
BslFI	GGGAC	1 cut(s)	1191
BslI	CCNNNNNNNGG	3 cut(s)	122, 595, 925
BsmAI	GTCTC	3 cut(s)	49, 394, 1100
BsmFI	GGGAC	1 cut(s)	1191
BsmI	GAATGC	1 cut(s)	219
BsnI	GGCC	2 cut(s)	181, 1533
Bso31I	GGTCTC	2 cut(s)	49, 1100
Bsp119I	TTCGAA	1 cut(s)	788
Bsp1407I	TGTACA	1 cut(s)	946
Bsp143I	GATC	4 cut(s)	745, 826, 1264, 1502
BspACI	CCGC	8 cut(s)	138, 330, 411, 568, 577, 926, 1062, 1236
BspANI	GGCC	2 cut(s)	181, 1533
BspCNI	CTCAG	4 cut(s)	388, 640, 1130, 1204
BspLI	GGNNCC	4 cut(s)	27, 36, 600, 1114
BspMAI	CTGCAG	1 cut(s)	1366
BspMI	ACCTGC	1 cut(s)	196
BspOI	GCTAGC	1 cut(s)	257
BspPI	GGATC	2 cut(s)	1272, 1510
BspQI	GCTCTTC	1 cut(s)	71
BspT104I	TTCGAA	1 cut(s)	788
BspT107I	GGYRCC	2 cut(s)	25, 1112
BspTNI	GGTCTC	2 cut(s)	49, 1100
BsrDI	GCAATG	4 cut(s)	273, 457, 705, 1560
BsrFI	RCCGGY	1 cut(s)	122
BsrGI	TGTACA	1 cut(s)	946
BsrI	ACTGG	6 cut(s)	155, 461, 519, 602, 718, 1396
BssAI	RCCGGY	1 cut(s)	122
BssECI	CCNNGG	2 cut(s)	390, 954
BssMI	GATC	4 cut(s)	745, 826, 1264, 1502
BssNAI	GTATAC	1 cut(s)	624
Bst1107I	GTATAC	1 cut(s)	624
Bst2UI	CCWGG	6 cut(s)	210, 392, 486, 507, 666, 1539
Bst4CI	ACNGT	8 cut(s)	424, 859, 899, 946, 1307, 1325, 1343, 1420
Bst6I	CTCTTC	2 cut(s)	71, 651
BstAPI	GCANNNNNTGC	3 cut(s)	197, 437, 1196
BstAUI	TGTACA	1 cut(s)	946
BstBI	TTCGAA	1 cut(s)	788
BstC8I	GCNNGC	6 cut(s)	255, 334, 493, 936, 1366, 1535
BstDEI	CTNAG	5 cut(s)	396, 627, 776, 1117, 1212
BstDSI	CCRYGG	1 cut(s)	954
BstEII	GGTNACC	1 cut(s)	118
BstF5I	GGATG	6 cut(s)	373, 640, 829, 1084, 1147, 1383
BstHHI	GCGC	3 cut(s)	11, 760, 1190
BstKTI	GATC	4 cut(s)	748, 829, 1267, 1505
BstMAI	GTCTC	3 cut(s)	49, 394, 1100
BstMBI	GATC	4 cut(s)	745, 826, 1264, 1502
BstMCI	CGRYCG	1 cut(s)	1236
BstMWI	GCNNNNNNNGC	9 cut(s)	34, 197, 263, 437, 574, 1196, 1238, 1530, 1539
BstNI	CCWGG	6 cut(s)	210, 392, 486, 507, 666, 1539
BstNSI	RCATGY	2 cut(s)	953, 1184
BstPI	GGTNACC	1 cut(s)	118
BstSCI	CCNGG	8 cut(s)	56, 114, 208, 390, 484, 505, 664, 1537
BstSFI	CTRYAG	3 cut(s)	898, 1339, 1362
BstV1I	GCAGC	6 cut(s)	184, 424, 577, 1250, 1345, 1348
BstX2I	RGATCY	1 cut(s)	1264
BstXI	CCANNNNNNTGG	1 cut(s)	452
BstYI	RGATCY	1 cut(s)	1264
BstZ17I	GTATAC	1 cut(s)	624
BsuI	GTATCC	1 cut(s)	533
BsuRI	GGCC	2 cut(s)	181, 1533
BtgI	CCRYGG	1 cut(s)	954
BtsCI	GGATG	6 cut(s)	373, 640, 829, 1084, 1147, 1383
BtsIMutI	CAGTG	4 cut(s)	876, 942, 1348, 1416
BveI	ACCTGC	1 cut(s)	196
Cac8I	GCNNGC	6 cut(s)	255, 334, 493, 936, 1366, 1535
CaiI	CAGNNNCTG	2 cut(s)	467, 1370
CfoI	GCGC	3 cut(s)	11, 760, 1190
Cfr10I	RCCGGY	1 cut(s)	122
Cfr13I	GGNCC	4 cut(s)	180, 482, 916, 1510
CseI	GACGC	2 cut(s)	1052, 1387
CsiI	ACCWGGT	1 cut(s)	484
Csp6I	GTAC	2 cut(s)	947, 990
CspAI	ACCGGT	1 cut(s)	122
CviAII	CATG	5 cut(s)	308, 323, 950, 993, 1181
CviQI	GTAC	2 cut(s)	947, 990
DdeI	CTNAG	5 cut(s)	396, 627, 776, 1117, 1212
DpnI	GATC	4 cut(s)	747, 828, 1266, 1504
DpnII	GATC	4 cut(s)	745, 826, 1264, 1502
Eam1104I	CTCTTC	2 cut(s)	71, 651
EarI	CTCTTC	2 cut(s)	71, 651
Eco31I	GGTCTC	2 cut(s)	49, 1100
Eco32I	GATATC	3 cut(s)	148, 585, 633
Eco47I	GGWCC	3 cut(s)	482, 916, 1510
Eco91I	GGTNACC	1 cut(s)	118
EcoO109I	RGGNCCY	1 cut(s)	482
EcoO65I	GGTNACC	1 cut(s)	118
EcoRII	CCWGG	6 cut(s)	208, 390, 484, 505, 664, 1537
EcoRV	GATATC	3 cut(s)	148, 585, 633
FaeI	CATG	5 cut(s)	311, 326, 953, 996, 1184
FaqI	GGGAC	1 cut(s)	1191
FatI	CATG	5 cut(s)	307, 322, 949, 992, 1180
FauI	CCCGC	1 cut(s)	418
FauNDI	CATATG	1 cut(s)	866
FbaI	TGATCA	1 cut(s)	745
FblI	GTMKAC	1 cut(s)	623
Fnu4HI	GCNGC	9 cut(s)	198, 330, 438, 566, 578, 1063, 1239, 1359, 1362
FokI	GGATG	6 cut(s)	380, 627, 836, 1071, 1154, 1370
Fsp4HI	GCNGC	9 cut(s)	198, 330, 438, 566, 578, 1063, 1239, 1359, 1362
FspBI	CTAG	5 cut(s)	201, 254, 527, 636, 1242
FspI	TGCGCA	1 cut(s)	10
GlaI	GCGC	3 cut(s)	10, 759, 1189
GluI	GCNGC	9 cut(s)	198, 330, 438, 566, 578, 1063, 1239, 1359, 1362
GsuI	CTGGAG	2 cut(s)	585, 735
HaeIII	GGCC	2 cut(s)	181, 1533
HapII	CCGG	5 cut(s)	18, 29, 57, 116, 123
HgaI	GACGC	2 cut(s)	1052, 1387
HhaI	GCGC	3 cut(s)	11, 760, 1190
Hin1II	CATG	5 cut(s)	311, 326, 953, 996, 1184
Hin6I	GCGC	3 cut(s)	9, 758, 1188
HinP1I	GCGC	3 cut(s)	9, 758, 1188
HinfI	GANTC	4 cut(s)	304, 351, 1380, 1559
HpaII	CCGG	5 cut(s)	18, 29, 57, 116, 123
HphI	GGTGA	2 cut(s)	137, 971
Hpy166II	GTNNAC	3 cut(s)	128, 624, 1296
Hpy188I	TCNGA	6 cut(s)	100, 630, 1057, 1120, 1213, 1372
Hpy188III	TCNNGA	6 cut(s)	70, 821, 983, 1088, 1288, 1563
Hpy8I	GTNNAC	3 cut(s)	128, 624, 1296
Hpy99I	CGWCG	1 cut(s)	1547
HpyAV	CCTTC	5 cut(s)	787, 907, 1052, 1216, 1529
HpyCH4III	ACNGT	8 cut(s)	424, 859, 899, 946, 1307, 1325, 1343, 1420
HpyF10VI	GCNNNNNNNGC	9 cut(s)	34, 197, 263, 437, 574, 1196, 1238, 1530, 1539
HpyF3I	CTNAG	5 cut(s)	396, 627, 776, 1117, 1212
Hsp92II	CATG	5 cut(s)	311, 326, 953, 996, 1184
HspAI	GCGC	3 cut(s)	9, 758, 1188
Ksp22I	TGATCA	1 cut(s)	745
Kzo9I	GATC	4 cut(s)	745, 826, 1264, 1502
LguI	GCTCTTC	1 cut(s)	71
LmnI	GCTCC	2 cut(s)	34, 604
Lsp1109I	GCAGC	6 cut(s)	184, 424, 577, 1250, 1345, 1348
LweI	GCATC	6 cut(s)	240, 316, 564, 598, 1022, 1471
MabI	ACCWGGT	1 cut(s)	484
MaeI	CTAG	5 cut(s)	201, 254, 527, 636, 1242
MaeIII	GTNAC	8 cut(s)	118, 424, 550, 796, 940, 959, 970, 1220
MalI	GATC	4 cut(s)	747, 828, 1266, 1504
MboI	GATC	4 cut(s)	745, 826, 1264, 1502
MboII	GAAGA	6 cut(s)	58, 181, 544, 583, 638, 802
MfeI	CAATTG	1 cut(s)	678
MflI	RGATCY	1 cut(s)	1264
MluCI	AATT	9 cut(s)	405, 669, 678, 809, 1134, 1274, 1331, 1348, 1486
MlyI	GAGTC	2 cut(s)	313, 345
MmeI	TCCRAC	1 cut(s)	594
MseI	TTAA	2 cut(s)	80, 1254
MslI	CAYNNNNRTG	2 cut(s)	450, 1089
MspA1I	CMGCKG	2 cut(s)	140, 568
MspI	CCGG	5 cut(s)	18, 29, 57, 116, 123
MspR9I	CCNGG	8 cut(s)	58, 116, 210, 392, 486, 507, 666, 1539
MunI	CAATTG	1 cut(s)	678
Mva1269I	GAATGC	1 cut(s)	219
MvaI	CCWGG	6 cut(s)	210, 392, 486, 507, 666, 1539
MwoI	GCNNNNNNNGC	9 cut(s)	34, 197, 263, 437, 574, 1196, 1238, 1530, 1539
NciI	CCSGG	2 cut(s)	58, 116
NdeI	CATATG	1 cut(s)	866
NdeII	GATC	4 cut(s)	745, 826, 1264, 1502
NheI	GCTAGC	1 cut(s)	253
NlaIII	CATG	5 cut(s)	311, 326, 953, 996, 1184
NlaIV	GGNNCC	4 cut(s)	27, 36, 600, 1114
NmuCI	GTSAC	3 cut(s)	940, 959, 970
NsbI	TGCGCA	1 cut(s)	10
NspI	RCATGY	2 cut(s)	953, 1184
NspV	TTCGAA	1 cut(s)	788
PciI	ACATGT	1 cut(s)	1180
PciSI	GCTCTTC	1 cut(s)	71
PctI	GAATGC	1 cut(s)	219
PfeI	GAWTC	1 cut(s)	1380
PfoI	TCCNGGA	1 cut(s)	208
PinAI	ACCGGT	1 cut(s)	122
PkrI	GCNGC	9 cut(s)	199, 331, 439, 567, 579, 1064, 1240, 1360, 1363
PleI	GAGTC	2 cut(s)	312, 345
PpsI	GAGTC	2 cut(s)	312, 345
PpuMI	RGGWCCY	1 cut(s)	482
PscI	ACATGT	1 cut(s)	1180
Psp5II	RGGWCCY	1 cut(s)	482
Psp6I	CCWGG	6 cut(s)	208, 390, 484, 505, 664, 1537
PspEI	GGTNACC	1 cut(s)	118
PspGI	CCWGG	6 cut(s)	208, 390, 484, 505, 664, 1537
PspN4I	GGNNCC	4 cut(s)	27, 36, 600, 1114
PspPI	GGNCC	4 cut(s)	180, 482, 916, 1510
PspPPI	RGGWCCY	1 cut(s)	482
PstI	CTGCAG	1 cut(s)	1366
PstNI	CAGNNNCTG	2 cut(s)	467, 1370
PsuI	RGATCY	1 cut(s)	1264
RsaI	GTAC	2 cut(s)	948, 991
RsaNI	GTAC	2 cut(s)	947, 990
RseI	CAYNNNNRTG	2 cut(s)	450, 1089
SapI	GCTCTTC	1 cut(s)	71
SaqAI	TTAA	2 cut(s)	80, 1254
SatI	GCNGC	9 cut(s)	198, 330, 438, 566, 578, 1063, 1239, 1359, 1362
Sau3AI	GATC	4 cut(s)	745, 826, 1264, 1502
Sau96I	GGNCC	4 cut(s)	180, 482, 916, 1510
SchI	GAGTC	2 cut(s)	313, 345
ScrFI	CCNGG	8 cut(s)	58, 116, 210, 392, 486, 507, 666, 1539
SexAI	ACCWGGT	1 cut(s)	484
SfaNI	GCATC	6 cut(s)	240, 316, 564, 598, 1022, 1471
SfcI	CTRYAG	3 cut(s)	898, 1339, 1362
SfuI	TTCGAA	1 cut(s)	788
SinI	GGWCC	3 cut(s)	482, 916, 1510
SmiMI	CAYNNNNRTG	2 cut(s)	450, 1089
Sse9I	AATT	9 cut(s)	405, 669, 678, 809, 1134, 1274, 1331, 1348, 1486
SsiI	CCGC	8 cut(s)	138, 330, 411, 568, 577, 926, 1062, 1236
SspMI	CTAG	5 cut(s)	201, 254, 527, 636, 1242
StyD4I	CCNGG	8 cut(s)	56, 114, 208, 390, 484, 505, 664, 1537
TaaI	ACNGT	8 cut(s)	424, 859, 899, 946, 1307, 1325, 1343, 1420
TaqI	TCGA	3 cut(s)	69, 84, 788
TaqII	GACCGA	2 cut(s)	554, 1527
TasI	AATT	9 cut(s)	405, 669, 678, 809, 1134, 1274, 1331, 1348, 1486
TatI	WGTACW	2 cut(s)	946, 989
TauI	GCSGC	3 cut(s)	332, 580, 1065
TfiI	GAWTC	1 cut(s)	1380
Tru1I	TTAA	2 cut(s)	80, 1254
Tru9I	TTAA	2 cut(s)	80, 1254
TscAI	CASTG	4 cut(s)	883, 949, 1348, 1423
TseFI	GTSAC	3 cut(s)	940, 959, 970
TseI	GCWGC	6 cut(s)	197, 437, 565, 1238, 1358, 1361
Tsp45I	GTSAC	3 cut(s)	940, 959, 970
TspDTI	ATGAA	5 cut(s)	17, 384, 638, 823, 1073
TspRI	CASTG	4 cut(s)	883, 949, 1348, 1423
VpaK11BI	GGWCC	3 cut(s)	482, 916, 1510
XapI	RAATTY	5 cut(s)	405, 669, 809, 1134, 1331
XceI	RCATGY	2 cut(s)	953, 1184
XcmI	CCANNNNNNNNNTGG	2 cut(s)	265, 482
XmiI	GTMKAC	1 cut(s)	623
XspI	CTAG	5 cut(s)	201, 254, 527, 636, 1242

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.