Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008688

Physical Location & Seq

Genomic Coordinates Forward (+)

22847532 .. 22856828

Gene Structure 9297 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1225900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGAACTGCGCACTATCCGGCGAGGTGCCGGAAGAGCCTGTGGTCTCCAAGAAGTCCGGGTTGCTCTTCGAGAAGCGTTTAATCGAGCGACATATCTCGGAATATGGGAAATGCCCGGTAACCGGTGAACCACTTTCCGCTGATGATATCGTTCCAGTTCAAACGGGGAAGATAGTGAGGCCCCGACCTGCACAAGCTGCTAGTATTCCTGGAATGCTCGGAATGTTCCAGCTTGAATGGGACAGTTTGATGCTAGCCAACTTTGCAATGGAGAAACAACTACATACAGCAAGGCAAGAGTTGAGTCATGCTTTGTATCAGCATGATGCTGCATGCCGTGTTATTGCAAGACTCAAAAAAGAAAGGGACGAAGCAAGGTCATTACTTGCTCAAGCTGAGAGACAAATTCCCGCAGCACAATCAGTAGTTGCAGTAAATGCTTCTGTCCATAGCAATGGAACGCCAGTTGCTGATGACCAGCAGGGACCTGGTGCGAGCAATATACGACCTGGAATATCTGCCAGTATCCTAGAGAAAATGACCGAATGTAATGTAACTCTTTCACAGCAGCGGAAGATGCGGCAGATATCCCCAACATTAGCTCCAGTTGATGCGTTGGAGAGGTATACTCTGATATCTAGTCATCCTCTTCATAGAACAAGCAAACCAGGAATTCTATCGATTGATATTCAGTCTAACAAGAATCTCATTGCAACTGGAGGGGTTGATACAAATGCTGTAATCTTTGATCAGCCATCAGGGCAAATAATATCTACTCTTAGTGGTCATTCAAAGAAGGTTACAAGCGTGAAATTTGTAGGTCGGGATGATATGTTCATAAGTGGGTCAGCAGACAAGACAGTTCGCATATGGCAAGGCTCTGATGATGGGAACTATAACTGTAGGCACATTTTGAAGGACCATACTGCTGAGGTGCAAGCTGTTACTGTGCATGCCACTGGGGACTACTTTGTGACTGCTTCTCTTGATAGTACATGGTGCTTTTACAATATTGCCTCTGGGTTATGTCTAACACAGGTTGAGGATGCTTCTGCTTCGGAAGGCTATACATCCGTTGCATTTCATCCTGATGGTCTCATTCTTGGAACTGGCACCTCAGAGGCTCTTGTGAAAATTTGGGATGTAAAGGGTCAGTCAAATGTTGCAACATTTGAGGGACATGTTGGCGCAATAACTGCAATCGCCTTCTCTGAGAATGGTTACTTTCTTGCGACTGCAGCTAGTGATGGCATTAAGTTGTGGGATCTACGAAAATTGAGGAACTTCAAGAAATTTACTCCCTACGATGCTGAACCAACAAACTGTGTTGAATTTGACTACAGTGGAAATTACCTTGCTGCTGCAGGCTCTGATATAAGAATCATCCAAACAGCTAGCGTCAAAAGTGAATGGAACACTATAAAGACTTTCCCTGACATGTCTGGCACAGGTAAAGCAACAAGTGTGAAGTTTGGACCAGATGCAAATTATATTGCAGTAGGATCTATGGACAGAAACCTTCGCATTTTTGGCCTGCCCGGCGTAGATGGTGCAATGGAGTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0000349 GO:0000375 GO:0000377 GO:0000393 GO:0000398 GO:0000974 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008380 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022607 GO:0022613 GO:0022618 GO:0030312 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0034622 GO:0034641 GO:0036211 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0061630 GO:0061659 GO:0065003 GO:0070013 GO:0070534 GO:0070647 GO:0071006 GO:0071012 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0080008 GO:0090304 GO:0098542 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 ko04120 map03040 map04120

Pfam Domains

Protein Families

Prp19 U-box WD40

Protein Analysis

522

Amino Acids

56.32

Weight (kDa)

6.03

Isoelectric Point (pI)

30.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Prp19	PF08606	66 - 131	6.4e-29	Prp19/Pso4-like
WD40_Prp19	PF24814	217 - 504	2.1e-107	Prp19 WD40 domain
Beta-prop_DCAF12	PF23760	220 - 297	1.1e-06	DDB1- and CUL4-associated factor 12 beta propeller
Beta-prop_WDR5	PF25175	221 - 365	5.6e-24	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	224 - 338	8.7e-19	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	224 - 346	3.3e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	224 - 368	7.1e-13	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	225 - 300	3.1e-09	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	225 - 422	2.2e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd	PF25172	225 - 336	2e-15	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	230 - 348	1.2e-10	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	231 - 421	3.4e-17	MABP1/WDR62 second WD40 domain
Beta-prop_WDR19_1st	PF23389	233 - 456	2.6e-06	WDR19 first beta-propeller
WD40	PF00400	255 - 291	9.3e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	257 - 351	6.9e-08	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	260 - 351	2.8e-07	EIF3I
Beta-prop_THOC3	PF25174	263 - 510	2.4e-38	THOC3 beta-propeller domain
WDR55	PF24796	265 - 439	1.3e-15	WDR55
Beta-prop_TEP1_2nd	PF25047	299 - 424	3.8e-07	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	306 - 457	5.3e-14	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	313 - 431	1.9e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	323 - 417	3.7e-09	WDR90/POC16, second beta-propeller
WD40_CDC20-Fz	PF24807	327 - 459	2e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	333 - 458	9.7e-06	WDR36/Utp21 first beta-propeller
Beta-prop_WDR5	PF25175	353 - 510	2.9e-21	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	355 - 421	1.3e-06	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	356 - 432	8.7e-10	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	356 - 459	3e-14	WDR3 first beta-propeller domain
EIF3I	PF24805	357 - 508	6.3e-08	EIF3I
WD40_Gbeta	PF25391	357 - 510	2e-10	G protein beta WD-40 repeat protein
WD40	PF00400	387 - 422	2.7e-06	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	397 - 510	5.6e-06	CDC20/Fizzy WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008688)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04510 AT1G04510 AT2G33340 AT2G33340 AT2G33340
fragaria_vesca	FvH4_4g04520 FvH4_4g04520
malus_domestica	MD13G1220800.v1.1 MD16G1225900.v1.1
prunus_persica	Prupe.1G047500_v2.0.a1
pyrus_communis	pycom13g19530 pycom16g18900
rosa_chinensis	RchiOBHm_Chr4g0395291
rosa_laevigata	RLG00000009634
rosa_multiflora	Rmu_sc0015296.1_g000001 Rmu_sc0032966.1_g000001
rosa_roxburghii	Rroxscaffold_5G00340170
rosa_rugosa	Rorug03G0358300
rosa_samantha	Rh4AG058200 Rh4BG057000 Rh4CG062800 Rh4DG053700
rosa_wichuraiana	Rw4G004680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	10
Acc36I	ACCTGC	1 cut(s)	196
AccB1I	GGYRCC	2 cut(s)	25, 1112
AccI	GTMKAC	1 cut(s)	626
AciI	CCGC	4 cut(s)	138, 411, 571, 580
AclWI	GGATC	2 cut(s)	1272, 1510
AcsI	RAATTY	6 cut(s)	405, 672, 812, 1134, 1292, 1331
AfaI	GTAC	1 cut(s)	994
AfiI	CCNNNNNNNGG	1 cut(s)	122
AflIII	ACRYGT	2 cut(s)	1180, 1437
AgeI	ACCGGT	1 cut(s)	122
AgsI	TTSAA	6 cut(s)	161, 236, 792, 916, 1288, 1331
AjnI	CCWGG	4 cut(s)	208, 487, 508, 667
AloI	GAACNNNNNNTCC	2 cut(s)	818, 850
AluBI	AGCT	7 cut(s)	197, 232, 395, 602, 941, 1241, 1394
AluI	AGCT	7 cut(s)	197, 232, 395, 602, 941, 1241, 1394
Alw26I	GTCTC	3 cut(s)	49, 394, 1100
AlwI	GGATC	2 cut(s)	1272, 1510
AlwNI	CAGNNNCTG	2 cut(s)	470, 1370
AoxI	GGCC	2 cut(s)	179, 1531
ApeKI	GCWGC	7 cut(s)	197, 329, 413, 568, 1238, 1358, 1361
ApoI	RAATTY	6 cut(s)	405, 672, 812, 1134, 1292, 1331
AsiGI	ACCGGT	1 cut(s)	122
AspLEI	GCGC	2 cut(s)	11, 1190
AspS9I	GGNCC	4 cut(s)	180, 485, 919, 1475
AsuC2I	CCSGG	3 cut(s)	58, 116, 1539
AsuHPI	GGTGA	1 cut(s)	137
AsuNHI	GCTAGC	2 cut(s)	253, 1394
AvaII	GGWCC	3 cut(s)	485, 919, 1475
BanI	GGYRCC	2 cut(s)	25, 1112
BbvCI	CCTCAGC	1 cut(s)	930
BbvI	GCAGC	7 cut(s)	184, 316, 425, 580, 1250, 1345, 1348
BccI	CCATC	5 cut(s)	763, 881, 1085, 1241, 1541
BceAI	ACGGC	1 cut(s)	321
BciT130I	CCWGG	4 cut(s)	210, 489, 510, 669
BciVI	GTATCC	1 cut(s)	536
BclI	TGATCA	1 cut(s)	748
BcnI	CCSGG	3 cut(s)	58, 116, 1539
BcoDI	GTCTC	3 cut(s)	49, 394, 1100
BfaI	CTAG	6 cut(s)	201, 254, 530, 639, 1242, 1395
BfmI	CTRYAG	4 cut(s)	901, 1236, 1339, 1362
BfuAI	ACCTGC	1 cut(s)	196
BfuI	GTATCC	1 cut(s)	536
BglI	GCCNNNNNGGC	2 cut(s)	760, 1539
BisI	GCNGC	8 cut(s)	198, 330, 414, 569, 581, 1239, 1359, 1362
BlsI	GCNGC	8 cut(s)	199, 331, 415, 570, 582, 1240, 1360, 1363
Bme1390I	CCNGG	7 cut(s)	58, 116, 210, 489, 510, 669, 1539
Bme18I	GGWCC	3 cut(s)	485, 919, 1475
BmgT120I	GGNCC	4 cut(s)	180, 485, 919, 1475
BmiI	GGNNCC	4 cut(s)	27, 182, 486, 1114
BmrFI	CCNGG	7 cut(s)	58, 116, 210, 489, 510, 669, 1539
BmrI	ACTGGG	1 cut(s)	969
BmsI	GCATC	7 cut(s)	240, 316, 567, 601, 1036, 1297, 1471
BmtI	GCTAGC	2 cut(s)	257, 1398
BmuI	ACTGGG	1 cut(s)	969
BplI	GAGNNNNNCTC	2 cut(s)	613, 645
BpmI	CTGGAG	2 cut(s)	588, 738
Bpu10I	CCTNAGC	1 cut(s)	930
BpuEI	CTTGAG	1 cut(s)	375
BpuMI	CCSGG	3 cut(s)	58, 116, 1539
Bsa29I	ATCGAT	1 cut(s)	680
BsaI	GGTCTC	2 cut(s)	49, 1100
BsaWI	WCCGGW	1 cut(s)	122
BsaXI	ACNNNNNCTCC	2 cut(s)	586, 616
Bsc4I	CCNNNNNNNGG	1 cut(s)	122
Bse118I	RCCGGY	1 cut(s)	122
Bse1I	ACTGG	7 cut(s)	155, 464, 522, 605, 721, 964, 1114
Bse3DI	GCAATG	4 cut(s)	273, 460, 708, 1560
BseBI	CCWGG	4 cut(s)	210, 489, 510, 669
BseCI	ATCGAT	1 cut(s)	680
BseGI	GGATG	7 cut(s)	643, 832, 1051, 1070, 1084, 1147, 1383
BseLI	CCNNNNNNNGG	1 cut(s)	122
BseMI	GCAATG	4 cut(s)	273, 460, 708, 1560
BseMII	CTCAG	4 cut(s)	387, 921, 1131, 1203
BseNI	ACTGG	7 cut(s)	155, 464, 522, 605, 721, 964, 1114
BseXI	GCAGC	7 cut(s)	184, 316, 425, 580, 1250, 1345, 1348
BsgI	GTGCAG	1 cut(s)	174
BshFI	GGCC	2 cut(s)	181, 1533
BshNI	GGYRCC	2 cut(s)	25, 1112
BshTI	ACCGGT	1 cut(s)	122
BshVI	ATCGAT	1 cut(s)	680
BsiSI	CCGG	6 cut(s)	18, 29, 57, 116, 123, 1539
BslFI	GGGAC	5 cut(s)	254, 380, 498, 977, 1191
BslI	CCNNNNNNNGG	1 cut(s)	122
BsmAI	GTCTC	3 cut(s)	49, 394, 1100
BsmFI	GGGAC	5 cut(s)	254, 380, 498, 977, 1191
BsmI	GAATGC	1 cut(s)	219
BsnI	GGCC	2 cut(s)	181, 1533
Bso31I	GGTCTC	2 cut(s)	49, 1100
Bsp143I	GATC	3 cut(s)	748, 1264, 1502
BspACI	CCGC	4 cut(s)	138, 411, 571, 580
BspANI	GGCC	2 cut(s)	181, 1533
BspCNI	CTCAG	4 cut(s)	388, 922, 1130, 1204
BspDI	ATCGAT	1 cut(s)	680
BspLI	GGNNCC	4 cut(s)	27, 182, 486, 1114
BspMAI	CTGCAG	2 cut(s)	1240, 1366
BspMI	ACCTGC	1 cut(s)	196
BspOI	GCTAGC	2 cut(s)	257, 1398
BspPI	GGATC	2 cut(s)	1272, 1510
BspQI	GCTCTTC	2 cut(s)	27, 71
BspT107I	GGYRCC	2 cut(s)	25, 1112
BspTNI	GGTCTC	2 cut(s)	49, 1100
BsrDI	GCAATG	4 cut(s)	273, 460, 708, 1560
BsrFI	RCCGGY	1 cut(s)	122
BsrI	ACTGG	7 cut(s)	155, 464, 522, 605, 721, 964, 1114
BssAI	RCCGGY	1 cut(s)	122
BssMI	GATC	3 cut(s)	748, 1264, 1502
BssNAI	GTATAC	1 cut(s)	627
Bst1107I	GTATAC	1 cut(s)	627
Bst2UI	CCWGG	4 cut(s)	210, 489, 510, 669
Bst4CI	ACNGT	6 cut(s)	245, 862, 902, 949, 1325, 1343
Bst6I	CTCTTC	3 cut(s)	27, 71, 654
BstAPI	GCANNNNNTGC	3 cut(s)	197, 437, 1196
BstC8I	GCNNGC	8 cut(s)	255, 334, 496, 939, 954, 1366, 1396, 1535
BstDEI	CTNAG	5 cut(s)	396, 779, 930, 1117, 1212
BstEII	GGTNACC	1 cut(s)	118
BstF5I	GGATG	7 cut(s)	643, 832, 1051, 1070, 1084, 1147, 1383
BstHHI	GCGC	2 cut(s)	11, 1190
BstKTI	GATC	3 cut(s)	751, 1267, 1505
BstMAI	GTCTC	3 cut(s)	49, 394, 1100
BstMBI	GATC	3 cut(s)	748, 1264, 1502
BstNI	CCWGG	4 cut(s)	210, 489, 510, 669
BstNSI	RCATGY	4 cut(s)	336, 956, 1184, 1441
BstPI	GGTNACC	1 cut(s)	118
BstSCI	CCNGG	7 cut(s)	56, 114, 208, 487, 508, 667, 1537
BstSFI	CTRYAG	4 cut(s)	901, 1236, 1339, 1362
BstV1I	GCAGC	7 cut(s)	184, 316, 425, 580, 1250, 1345, 1348
BstX2I	RGATCY	2 cut(s)	1264, 1502
BstXI	CCANNNNNNTGG	1 cut(s)	455
BstYI	RGATCY	2 cut(s)	1264, 1502
BstZ17I	GTATAC	1 cut(s)	627
Bsu15I	ATCGAT	1 cut(s)	680
BsuI	GTATCC	1 cut(s)	536
BsuRI	GGCC	2 cut(s)	181, 1533
BsuTUI	ATCGAT	1 cut(s)	680
BtsCI	GGATG	7 cut(s)	643, 832, 1051, 1070, 1084, 1147, 1383
BtsIMutI	CAGTG	2 cut(s)	957, 1348
BveI	ACCTGC	1 cut(s)	196
Cac8I	GCNNGC	8 cut(s)	255, 334, 496, 939, 954, 1366, 1396, 1535
CaiI	CAGNNNCTG	2 cut(s)	470, 1370
CfoI	GCGC	2 cut(s)	11, 1190
Cfr10I	RCCGGY	1 cut(s)	122
Cfr13I	GGNCC	4 cut(s)	180, 485, 919, 1475
ClaI	ATCGAT	1 cut(s)	680
CseI	GACGC	1 cut(s)	1387
CsiI	ACCWGGT	1 cut(s)	487
Csp6I	GTAC	1 cut(s)	993
CspAI	ACCGGT	1 cut(s)	122
CviAII	CATG	7 cut(s)	308, 323, 333, 953, 996, 1181, 1438
CviQI	GTAC	1 cut(s)	993
DdeI	CTNAG	5 cut(s)	396, 779, 930, 1117, 1212
DpnI	GATC	3 cut(s)	750, 1266, 1504
DpnII	GATC	3 cut(s)	748, 1264, 1502
Eam1104I	CTCTTC	3 cut(s)	27, 71, 654
EarI	CTCTTC	3 cut(s)	27, 71, 654
Eco31I	GGTCTC	2 cut(s)	49, 1100
Eco32I	GATATC	3 cut(s)	148, 588, 636
Eco47I	GGWCC	3 cut(s)	485, 919, 1475
Eco91I	GGTNACC	1 cut(s)	118
EcoO109I	RGGNCCY	2 cut(s)	180, 485
EcoO65I	GGTNACC	1 cut(s)	118
EcoRI	GAATTC	1 cut(s)	672
EcoRII	CCWGG	4 cut(s)	208, 487, 508, 667
EcoRV	GATATC	3 cut(s)	148, 588, 636
FaeI	CATG	7 cut(s)	311, 326, 336, 956, 999, 1184, 1441
FaqI	GGGAC	5 cut(s)	254, 380, 498, 977, 1191
FatI	CATG	7 cut(s)	307, 322, 332, 952, 995, 1180, 1437
FauI	CCCGC	1 cut(s)	418
FauNDI	CATATG	1 cut(s)	869
FbaI	TGATCA	1 cut(s)	748
FblI	GTMKAC	1 cut(s)	626
Fnu4HI	GCNGC	8 cut(s)	198, 330, 414, 569, 581, 1239, 1359, 1362
FokI	GGATG	7 cut(s)	630, 839, 1057, 1058, 1071, 1154, 1370
Fsp4HI	GCNGC	8 cut(s)	198, 330, 414, 569, 581, 1239, 1359, 1362
FspBI	CTAG	6 cut(s)	201, 254, 530, 639, 1242, 1395
FspI	TGCGCA	1 cut(s)	10
GlaI	GCGC	2 cut(s)	10, 1189
GluI	GCNGC	8 cut(s)	198, 330, 414, 569, 581, 1239, 1359, 1362
GsuI	CTGGAG	2 cut(s)	588, 738
HaeIII	GGCC	2 cut(s)	181, 1533
HapII	CCGG	6 cut(s)	18, 29, 57, 116, 123, 1539
HgaI	GACGC	1 cut(s)	1387
HhaI	GCGC	2 cut(s)	11, 1190
Hin1II	CATG	7 cut(s)	311, 326, 336, 956, 999, 1184, 1441
Hin6I	GCGC	2 cut(s)	9, 1188
HinP1I	GCGC	2 cut(s)	9, 1188
HinfI	GANTC	5 cut(s)	304, 351, 703, 1380, 1559
HpaII	CCGG	6 cut(s)	18, 29, 57, 116, 123, 1539
HphI	GGTGA	1 cut(s)	137
Hpy166II	GTNNAC	2 cut(s)	128, 627
Hpy188I	TCNGA	8 cut(s)	100, 221, 633, 883, 1060, 1120, 1213, 1372
Hpy188III	TCNNGA	6 cut(s)	70, 824, 986, 1088, 1288, 1563
Hpy8I	GTNNAC	2 cut(s)	128, 627
HpyAV	CCTTC	5 cut(s)	790, 910, 1055, 1216, 1529
HpyCH4III	ACNGT	6 cut(s)	245, 862, 902, 949, 1325, 1343
HpyF3I	CTNAG	5 cut(s)	396, 779, 930, 1117, 1212
Hsp92II	CATG	7 cut(s)	311, 326, 336, 956, 999, 1184, 1441
HspAI	GCGC	2 cut(s)	9, 1188
Ksp22I	TGATCA	1 cut(s)	748
Kzo9I	GATC	3 cut(s)	748, 1264, 1502
LguI	GCTCTTC	2 cut(s)	27, 71
LmnI	GCTCC	1 cut(s)	607
Lsp1109I	GCAGC	7 cut(s)	184, 316, 425, 580, 1250, 1345, 1348
LweI	GCATC	7 cut(s)	240, 316, 567, 601, 1036, 1297, 1471
MabI	ACCWGGT	1 cut(s)	487
MaeI	CTAG	6 cut(s)	201, 254, 530, 639, 1242, 1395
MaeIII	GTNAC	6 cut(s)	118, 553, 799, 943, 973, 1220
MalI	GATC	3 cut(s)	750, 1266, 1504
MboI	GATC	3 cut(s)	748, 1264, 1502
MboII	GAAGA	5 cut(s)	44, 58, 181, 586, 641
MflI	RGATCY	2 cut(s)	1264, 1502
MluCI	AATT	9 cut(s)	405, 672, 812, 1134, 1274, 1292, 1331, 1348, 1486
MlyI	GAGTC	2 cut(s)	313, 345
MmeI	TCCRAC	1 cut(s)	597
MseI	TTAA	2 cut(s)	80, 1254
MslI	CAYNNNNRTG	2 cut(s)	453, 1089
MspA1I	CMGCKG	2 cut(s)	140, 571
MspI	CCGG	6 cut(s)	18, 29, 57, 116, 123, 1539
MspR9I	CCNGG	7 cut(s)	58, 116, 210, 489, 510, 669, 1539
Mva1269I	GAATGC	1 cut(s)	219
MvaI	CCWGG	4 cut(s)	210, 489, 510, 669
NciI	CCSGG	3 cut(s)	58, 116, 1539
NdeI	CATATG	1 cut(s)	869
NdeII	GATC	3 cut(s)	748, 1264, 1502
NheI	GCTAGC	2 cut(s)	253, 1394
NlaIII	CATG	7 cut(s)	311, 326, 336, 956, 999, 1184, 1441
NlaIV	GGNNCC	4 cut(s)	27, 182, 486, 1114
NmuCI	GTSAC	1 cut(s)	973
NsbI	TGCGCA	1 cut(s)	10
NspI	RCATGY	4 cut(s)	336, 956, 1184, 1441
PaeI	GCATGC	2 cut(s)	336, 956
PciI	ACATGT	2 cut(s)	1180, 1437
PciSI	GCTCTTC	2 cut(s)	27, 71
PctI	GAATGC	1 cut(s)	219
PfeI	GAWTC	2 cut(s)	703, 1380
PfoI	TCCNGGA	1 cut(s)	208
PinAI	ACCGGT	1 cut(s)	122
PkrI	GCNGC	8 cut(s)	199, 331, 415, 570, 582, 1240, 1360, 1363
PleI	GAGTC	2 cut(s)	312, 345
PpsI	GAGTC	2 cut(s)	312, 345
PpuMI	RGGWCCY	1 cut(s)	485
PscI	ACATGT	2 cut(s)	1180, 1437
Psp5II	RGGWCCY	1 cut(s)	485
Psp6I	CCWGG	4 cut(s)	208, 487, 508, 667
PspEI	GGTNACC	1 cut(s)	118
PspGI	CCWGG	4 cut(s)	208, 487, 508, 667
PspN4I	GGNNCC	4 cut(s)	27, 182, 486, 1114
PspPI	GGNCC	4 cut(s)	180, 485, 919, 1475
PspPPI	RGGWCCY	1 cut(s)	485
PstI	CTGCAG	2 cut(s)	1240, 1366
PstNI	CAGNNNCTG	2 cut(s)	470, 1370
PsuI	RGATCY	2 cut(s)	1264, 1502
RsaI	GTAC	1 cut(s)	994
RsaNI	GTAC	1 cut(s)	993
RseI	CAYNNNNRTG	2 cut(s)	453, 1089
SapI	GCTCTTC	2 cut(s)	27, 71
SaqAI	TTAA	2 cut(s)	80, 1254
SatI	GCNGC	8 cut(s)	198, 330, 414, 569, 581, 1239, 1359, 1362
Sau3AI	GATC	3 cut(s)	748, 1264, 1502
Sau96I	GGNCC	4 cut(s)	180, 485, 919, 1475
SchI	GAGTC	2 cut(s)	313, 345
ScrFI	CCNGG	7 cut(s)	58, 116, 210, 489, 510, 669, 1539
SexAI	ACCWGGT	1 cut(s)	487
SfaNI	GCATC	7 cut(s)	240, 316, 567, 601, 1036, 1297, 1471
SfcI	CTRYAG	4 cut(s)	901, 1236, 1339, 1362
SinI	GGWCC	3 cut(s)	485, 919, 1475
SmiMI	CAYNNNNRTG	2 cut(s)	453, 1089
SmlI	CTYRAG	1 cut(s)	390
SmoI	CTYRAG	1 cut(s)	390
SphI	GCATGC	2 cut(s)	336, 956
Sse9I	AATT	9 cut(s)	405, 672, 812, 1134, 1274, 1292, 1331, 1348, 1486
SsiI	CCGC	4 cut(s)	138, 411, 571, 580
SspI	AATATT	1 cut(s)	1012
SspMI	CTAG	6 cut(s)	201, 254, 530, 639, 1242, 1395
StyD4I	CCNGG	7 cut(s)	56, 114, 208, 487, 508, 667, 1537
TaaI	ACNGT	6 cut(s)	245, 862, 902, 949, 1325, 1343
TaqI	TCGA	3 cut(s)	69, 84, 680
TaqII	GACCGA	1 cut(s)	557
TasI	AATT	9 cut(s)	405, 672, 812, 1134, 1274, 1292, 1331, 1348, 1486
TatI	WGTACW	1 cut(s)	992
TauI	GCSGC	1 cut(s)	583
TfiI	GAWTC	2 cut(s)	703, 1380
Tru1I	TTAA	2 cut(s)	80, 1254
Tru9I	TTAA	2 cut(s)	80, 1254
TscAI	CASTG	2 cut(s)	964, 1348
TseFI	GTSAC	1 cut(s)	973
TseI	GCWGC	7 cut(s)	197, 329, 413, 568, 1238, 1358, 1361
Tsp45I	GTSAC	1 cut(s)	973
TspDTI	ATGAA	4 cut(s)	17, 641, 826, 1073
TspGWI	ACGGA	1 cut(s)	1063
TspRI	CASTG	2 cut(s)	964, 1348
VpaK11BI	GGWCC	3 cut(s)	485, 919, 1475
XapI	RAATTY	6 cut(s)	405, 672, 812, 1134, 1292, 1331
XceI	RCATGY	4 cut(s)	336, 956, 1184, 1441
XcmI	CCANNNNNNNNNTGG	2 cut(s)	265, 485
XmiI	GTMKAC	1 cut(s)	626
XspI	CTAG	6 cut(s)	201, 254, 530, 639, 1242, 1395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.