Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011741

Physical Location & Seq

Genomic Coordinates Reverse (-)

22035829 .. 22037346

Gene Structure 1518 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1225400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGGCGACGTGGGCAACACTAGACTGCTCGGCTACCCAGTTGGCCGACCTCCTTGGGGGGCCCAACTCCACCGGCGCAGCCAACTTCATCTGCAACCAAATCGCCGGCGTCTCCTCCAAGCTCTCCGACACCGCCTACGCCGTCGACACCACCTTCCTCCTCTTCTCCGCCTACCTCGTCTTCTCAATGCAGCTCGGCTTCGCCATGCTCTGCGCCGGCTCCGTCCGCGCCAAGAACACCATGAACATCATGCTCACCAACGTCCTCGACGCCGCCGCCGGCGGCCTCTTCTACTACCTCTTCGGCTTCGCCTTCGCCTTTGGTGGCCCCTCCAACGGATTCATCGGCAAACACTTCTTCGGCCTCAAAGAGGTCCCGTCCAACACCGGTTTCGACTACAGCCACTTCCTCTACCAGTGGGCCTTCGCCATCGCCGCCGCCGGAATCACCAGCGGATCCATTGCCGAGAGGACTCAGTTCGTCGCCTACCTCATTTACTCCTCCTTCCTCACCGGATTCGTCTACCCGGTCGTCGCCCACTGGTTCTGGTCAGTCGATGGCTGGGCTAGTGCCTTAAATACCGGAAACCTCTTATTTGGAACCGGTGTCATCGATTTCGCCGGGTCGGGTGTGGTCCATATGGTCGGAGGCATCGCCGGTCTCTGGGGAGCTTTCATCGAAGGCCCCCGAATCGGGCGGTTCGACCACTCCGGCCGGGCCGTTGCTCTCCGCGGACACAGCGCTTCTCTCGTCGTTCTGGGCACATTTTTACTCTGGTTCGGGTGGTACGGGTTTAACCCCGGTTCGTTTACCAAGATCCTGAGTCCGTACGGGTCATCCGGATCCTATTACGGGATGTGGTCAGCTGTGGGTCGGACCGCTGTCACCACAACTCTCGCCGGATGCACCGCCGCTCTCACCACTCTCTTCGGAAAAAGAATTCTGTCGGGTCACTGGAACGTAACGGACGTCTGTAACGGCCTTCTCGGCGGATTCGCGGCGATAACTGCTGGATGTTCTGTCGTCGAACCGTGGGCGGCGATCGTATGCGGGTTCGTGGCCGCTATCGTTTTGATCTCGTGCAACAAACTGGCCGAGAAGTTGAAATTCGACGACCCGCTGGAGGCGGCGCAGCTTCACGGAGGGTGCGGCGCGTGGGGGATAATTTTCACGGCGCTGTTTGCGAGGGAGAAGTACGTGAACGAGGTGTACCCGGGAAAAGAGGGGCGGCCATACGGGCTGTTTATGGGCGGCGGAGGGAAGCTGCTGGGGGCCCACTTGATACAGATAGTGGTAATTATAGGGTGGGTGAGCGCGACGATGGGGCCGTTGTTCTTTGTGCTGCACAAGCTGAAGCTGCTGAGGATATCGGCGGAGGACGAAATGGCGGGTATGGATCTGACCCGGCACGGGGGATTTGCTTACGTGTACCATGACGACGAAGACGCGCAGAAGCCGAGCGTGATTCAGCTTAGAAAAGTAGAACCGAACGCTTCCACGCCGCCGACTTCTGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0001763 GO:0003674 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0007275 GO:0008150 GO:0008324 GO:0008519 GO:0009506 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0015075 GO:0015695 GO:0015696 GO:0016020 GO:0016021 GO:0016043 GO:0022607 GO:0022622 GO:0022857 GO:0030054 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0033993 GO:0034220 GO:0034622 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0043933 GO:0044085 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0048364 GO:0048527 GO:0048528 GO:0048646 GO:0048731 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0051258 GO:0055044 GO:0055085 GO:0065003 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072488 GO:0080167 GO:0080181 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0098655 GO:0099402 GO:1901700 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ammonium_transp

Protein Analysis

506

Amino Acids

53.7

Weight (kDa)

6.85

Isoelectric Point (pI)

16.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ammonium_transp	PF00909	52 - 475	4.4e-133	Ammonium Transporter Family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011741)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24290 AT3G24300 AT4G13510
fragaria_vesca	FvH4_4g05160
malus_domestica	MD13G1225400.v1.1 MD16G1230500.v1.1
prunus_persica	Prupe.1G052400_v2.0.a1
pyrus_communis	pycom13g19870 pycom13g19880
rosa_chinensis	RchiOBHm_Chr4g0396081
rosa_laevigata	RLG00000009574
rosa_multiflora	Rmu_sc0006325.1_g000013 Rmu_sc0015594.1_g000001
rosa_roxburghii	Rroxscaffold_5G00340880
rosa_rugosa	Rorug03G0352700
rosa_samantha	Rh4BG064000 Rh4CG070500 Rh4DG060800
rosa_wichuraiana	Rw4G005300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	972
AccBSI	CCGCTC	1 cut(s)	914
AccI	GTMKAC	2 cut(s)	144, 522
AccII	CGCG	6 cut(s)	228, 732, 998, 1154, 1316, 1448
AccIII	TCCGGA	1 cut(s)	839
AclWI	GGATC	6 cut(s)	450, 463, 811, 837, 850, 1404
AcoI	YGGCCR	5 cut(s)	42, 712, 1059, 1092, 1229
AcsI	RAATTY	2 cut(s)	939, 1106
AcuI	CTGAAG	1 cut(s)	1373
AcyI	GRCGYC	3 cut(s)	108, 270, 969
AfaI	GTAC	5 cut(s)	788, 830, 1196, 1211, 1430
AfeI	AGCGCT	1 cut(s)	742
AflIII	ACRYGT	1 cut(s)	1425
AgeI	ACCGGT	2 cut(s)	386, 602
AgsI	TTSAA	1 cut(s)	1105
AjiI	CACGTC	1 cut(s)	9
AjuI	GAANNNNNNNTTGG	2 cut(s)	374, 406
AluBI	AGCT	9 cut(s)	121, 193, 671, 866, 1135, 1264, 1351, 1357, 1471
AluI	AGCT	9 cut(s)	121, 193, 671, 866, 1135, 1264, 1351, 1357, 1471
Alw26I	GTCTC	2 cut(s)	115, 665
AlwI	GGATC	6 cut(s)	450, 463, 811, 837, 850, 1404
Ama87I	CYCGRG	1 cut(s)	1213
Aor13HI	TCCGGA	1 cut(s)	839
Aor51HI	AGCGCT	1 cut(s)	742
ApaI	GGGCCC	2 cut(s)	63, 1276
ApeKI	GCWGC	6 cut(s)	77, 190, 1132, 1264, 1342, 1357
ApoI	RAATTY	2 cut(s)	939, 1106
AsiGI	ACCGGT	2 cut(s)	386, 602
AspLEI	GCGC	9 cut(s)	77, 215, 230, 743, 1132, 1154, 1177, 1316, 1450
AsuC2I	CCSGG	7 cut(s)	527, 622, 716, 801, 1214, 1215, 1405
AsuHPI	GGTGA	6 cut(s)	247, 439, 502, 877, 910, 1321
AvaI	CYCGRG	1 cut(s)	1213
AvaII	GGWCC	3 cut(s)	373, 634, 876
BaeGI	GKGCMC	3 cut(s)	63, 764, 1276
BamHI	GGATCC	2 cut(s)	455, 842
BanII	GRGCYC	2 cut(s)	63, 1276
BauI	CACGAG	1 cut(s)	1078
BbsI	GAAGAC	2 cut(s)	172, 1449
BbvCI	CCTCAGC	1 cut(s)	1361
BbvI	GCAGC	6 cut(s)	89, 202, 1144, 1251, 1329, 1344
BccI	CCATC	3 cut(s)	437, 551, 1315
BceAI	ACGGC	5 cut(s)	125, 704, 994, 1188, 1312
BcgI	CGANNNNNNTGC	2 cut(s)	82, 116
BcnI	CCSGG	7 cut(s)	527, 622, 716, 801, 1214, 1215, 1405
BcoDI	GTCTC	2 cut(s)	115, 665
BfaI	CTAG	2 cut(s)	20, 567
BfmI	CTRYAG	1 cut(s)	397
BfoI	RGCGCY	2 cut(s)	744, 1178
BglI	GCCNNNNNGGC	2 cut(s)	987, 1237
Bme1390I	CCNGG	7 cut(s)	527, 622, 716, 801, 1214, 1215, 1405
Bme18I	GGWCC	3 cut(s)	373, 634, 876
BmeT110I	CYCGRG	1 cut(s)	1213
BmgBI	CACGTC	1 cut(s)	9
BmrFI	CCNGG	7 cut(s)	527, 622, 716, 801, 1214, 1215, 1405
BmrI	ACTGGG	1 cut(s)	31
BmsI	GCATC	2 cut(s)	660, 893
BmuI	ACTGGG	1 cut(s)	31
BoxI	GACNNNNGTC	1 cut(s)	881
BpiI	GAAGAC	2 cut(s)	172, 1449
BpmI	CTGGAG	1 cut(s)	1142
Bpu10I	CCTNAGC	1 cut(s)	1361
BpuMI	CCSGG	7 cut(s)	527, 622, 716, 801, 1214, 1215, 1405
Bsa29I	ATCGAT	1 cut(s)	612
BsaAI	YACGTR	2 cut(s)	1198, 1426
BsaHI	GRCGYC	3 cut(s)	108, 270, 969
BsaI	GGTCTC	1 cut(s)	665
BsaJI	CCNNGG	5 cut(s)	52, 730, 799, 1031, 1213
BsaWI	WCCGGW	5 cut(s)	386, 512, 581, 602, 839
Bse118I	RCCGGY	7 cut(s)	71, 104, 215, 278, 386, 602, 656
Bse1I	ACTGG	5 cut(s)	37, 415, 545, 959, 1095
Bse3DI	GCAATG	1 cut(s)	459
BseAI	TCCGGA	1 cut(s)	839
BseCI	ATCGAT	1 cut(s)	612
BseDI	CCNNGG	5 cut(s)	52, 730, 799, 1031, 1213
BseGI	GGATG	4 cut(s)	836, 861, 908, 1019
BseMI	GCAATG	1 cut(s)	459
BseMII	CTCAG	3 cut(s)	488, 812, 1352
BseNI	ACTGG	5 cut(s)	37, 415, 545, 959, 1095
BseRI	GAGGAG	3 cut(s)	103, 149, 490
BseSI	GKGCMC	3 cut(s)	63, 764, 1276
BseX3I	CGGCCG	1 cut(s)	712
BseXI	GCAGC	6 cut(s)	89, 202, 1144, 1251, 1329, 1344
BseYI	CCCAGC	2 cut(s)	561, 1267
BsgI	GTGCAG	1 cut(s)	1328
Bsh1236I	CGCG	6 cut(s)	228, 732, 998, 1154, 1316, 1448
Bsh1285I	CGRYCG	3 cut(s)	531, 715, 1044
BshTI	ACCGGT	2 cut(s)	386, 602
BshVI	ATCGAT	1 cut(s)	612
BsiEI	CGRYCG	3 cut(s)	531, 715, 1044
BsiHKCI	CYCGRG	1 cut(s)	1213
BsiWI	CGTACG	1 cut(s)	828
BslFI	GGGAC	1 cut(s)	359
BsmAI	GTCTC	2 cut(s)	115, 665
BsmBI	CGTCTC	1 cut(s)	115
BsmFI	GGGAC	1 cut(s)	359
Bso31I	GGTCTC	1 cut(s)	665
BsoBI	CYCGRG	1 cut(s)	1213
Bsp120I	GGGCCC	2 cut(s)	59, 1272
Bsp1286I	GDGCHC	3 cut(s)	63, 764, 1276
Bsp13I	TCCGGA	1 cut(s)	839
Bsp143I	GATC	6 cut(s)	455, 816, 842, 1041, 1074, 1396
BspCNI	CTCAG	3 cut(s)	487, 813, 1353
BspDI	ATCGAT	1 cut(s)	612
BspEI	TCCGGA	1 cut(s)	839
BspFNI	CGCG	6 cut(s)	228, 732, 998, 1154, 1316, 1448
BspPI	GGATC	6 cut(s)	450, 463, 811, 837, 850, 1404
BspTNI	GGTCTC	1 cut(s)	665
BsrBI	CCGCTC	1 cut(s)	914
BsrDI	GCAATG	1 cut(s)	459
BsrFI	RCCGGY	7 cut(s)	71, 104, 215, 278, 386, 602, 656
BsrI	ACTGG	5 cut(s)	37, 415, 545, 959, 1095
BssAI	RCCGGY	7 cut(s)	71, 104, 215, 278, 386, 602, 656
BssECI	CCNNGG	5 cut(s)	52, 730, 799, 1031, 1213
BssMI	GATC	6 cut(s)	455, 816, 842, 1041, 1074, 1396
BssNI	GRCGYC	3 cut(s)	108, 270, 969
BssSI	CACGAG	1 cut(s)	1078
BssT1I	CCWWGG	1 cut(s)	52
Bst2BI	CACGAG	1 cut(s)	1078
Bst4CI	ACNGT	1 cut(s)	1032
Bst6I	CTCTTC	4 cut(s)	167, 293, 305, 932
BstACI	GRCGYC	3 cut(s)	108, 270, 969
BstBAI	YACGTR	2 cut(s)	1198, 1426
BstC8I	GCNNGC	3 cut(s)	106, 217, 280
BstDEI	CTNAG	4 cut(s)	474, 821, 1361, 1472
BstDSI	CCRYGG	2 cut(s)	730, 1031
BstENI	CCTNNNNNAGG	1 cut(s)	368
BstF5I	GGATG	4 cut(s)	836, 861, 908, 1019
BstFNI	CGCG	6 cut(s)	228, 732, 998, 1154, 1316, 1448
BstH2I	RGCGCY	2 cut(s)	744, 1178
BstHHI	GCGC	9 cut(s)	77, 215, 230, 743, 1132, 1154, 1177, 1316, 1450
BstKTI	GATC	6 cut(s)	458, 819, 845, 1044, 1077, 1399
BstMAI	GTCTC	2 cut(s)	115, 665
BstMBI	GATC	6 cut(s)	455, 816, 842, 1041, 1074, 1396
BstMCI	CGRYCG	3 cut(s)	531, 715, 1044
BstPAI	GACNNNNGTC	1 cut(s)	881
BstSCI	CCNGG	7 cut(s)	525, 620, 714, 799, 1212, 1213, 1403
BstSFI	CTRYAG	1 cut(s)	397
BstSLI	GKGCMC	3 cut(s)	63, 764, 1276
BstUI	CGCG	6 cut(s)	228, 732, 998, 1154, 1316, 1448
BstV1I	GCAGC	6 cut(s)	89, 202, 1144, 1251, 1329, 1344
BstV2I	GAAGAC	2 cut(s)	172, 1449
BstX2I	RGATCY	4 cut(s)	455, 816, 842, 1396
BstYI	RGATCY	4 cut(s)	455, 816, 842, 1396
BstZI	CGGCCG	1 cut(s)	712
Bsu15I	ATCGAT	1 cut(s)	612
BsuTUI	ATCGAT	1 cut(s)	612
BtgI	CCRYGG	2 cut(s)	730, 1031
BtgZI	GCGATG	2 cut(s)	415, 637
BtrI	CACGTC	1 cut(s)	9
BtsCI	GGATG	4 cut(s)	836, 861, 908, 1019
BtsIMutI	CAGTG	3 cut(s)	422, 538, 952
Cac8I	GCNNGC	3 cut(s)	106, 217, 280
CfoI	GCGC	9 cut(s)	77, 215, 230, 743, 1132, 1154, 1177, 1316, 1450
Cfr10I	RCCGGY	7 cut(s)	71, 104, 215, 278, 386, 602, 656
Cfr42I	CCGCGG	1 cut(s)	733
Cfr9I	CCCGGG	1 cut(s)	1213
ClaI	ATCGAT	1 cut(s)	612
CpoI	CGGWCCG	1 cut(s)	876
CseI	GACGC	3 cut(s)	97, 278, 1454
Csp6I	GTAC	5 cut(s)	787, 829, 1195, 1210, 1429
CspAI	ACCGGT	2 cut(s)	386, 602
CspI	CGGWCCG	1 cut(s)	876
CviAII	CATG	4 cut(s)	205, 241, 250, 1433
CviQI	GTAC	5 cut(s)	787, 829, 1195, 1210, 1429
DdeI	CTNAG	4 cut(s)	474, 821, 1361, 1472
DpnI	GATC	6 cut(s)	457, 818, 844, 1043, 1076, 1398
DpnII	GATC	6 cut(s)	455, 816, 842, 1041, 1074, 1396
EaeI	YGGCCR	5 cut(s)	42, 712, 1059, 1092, 1229
EagI	CGGCCG	1 cut(s)	712
Eam1104I	CTCTTC	4 cut(s)	167, 293, 305, 932
EarI	CTCTTC	4 cut(s)	167, 293, 305, 932
EciI	GGCGGA	4 cut(s)	157, 1005, 1269, 1388
EclXI	CGGCCG	1 cut(s)	712
Eco130I	CCWWGG	1 cut(s)	52
Eco24I	GRGCYC	2 cut(s)	63, 1276
Eco31I	GGTCTC	1 cut(s)	665
Eco32I	GATATC	1 cut(s)	1368
Eco47I	GGWCC	3 cut(s)	373, 634, 876
Eco47III	AGCGCT	1 cut(s)	742
Eco52I	CGGCCG	1 cut(s)	712
Eco57I	CTGAAG	1 cut(s)	1373
Eco88I	CYCGRG	1 cut(s)	1213
EcoNI	CCTNNNNNAGG	1 cut(s)	368
EcoO109I	RGGNCCY	4 cut(s)	59, 373, 683, 1272
EcoRI	GAATTC	1 cut(s)	939
EcoRV	GATATC	1 cut(s)	1368
EcoT14I	CCWWGG	1 cut(s)	52
EcoT38I	GRGCYC	2 cut(s)	63, 1276
ErhI	CCWWGG	1 cut(s)	52
Esp3I	CGTCTC	1 cut(s)	115
FaeI	CATG	4 cut(s)	208, 244, 253, 1436
FaqI	GGGAC	1 cut(s)	359
FatI	CATG	4 cut(s)	204, 240, 249, 1432
FauI	CCCGC	3 cut(s)	1043, 1125, 1381
FauNDI	CATATG	1 cut(s)	639
FblI	GTMKAC	2 cut(s)	144, 522
FokI	GGATG	4 cut(s)	823, 868, 915, 1026
FriOI	GRGCYC	2 cut(s)	63, 1276
FspBI	CTAG	2 cut(s)	20, 567
GlaI	GCGC	9 cut(s)	76, 214, 229, 742, 1131, 1153, 1176, 1315, 1449
GsaI	CCCAGC	2 cut(s)	565, 1271
GsuI	CTGGAG	1 cut(s)	1142
HaeII	RGCGCY	2 cut(s)	744, 1178
HgaI	GACGC	3 cut(s)	97, 278, 1454
HhaI	GCGC	9 cut(s)	77, 215, 230, 743, 1132, 1154, 1177, 1316, 1450
Hin1I	GRCGYC	3 cut(s)	108, 270, 969
Hin1II	CATG	4 cut(s)	208, 244, 253, 1436
Hin6I	GCGC	9 cut(s)	75, 213, 228, 741, 1130, 1152, 1175, 1314, 1448
HinP1I	GCGC	9 cut(s)	75, 213, 228, 741, 1130, 1152, 1175, 1314, 1448
HincII	GTYRAC	1 cut(s)	145
HindII	GTYRAC	1 cut(s)	145
HinfI	GANTC	8 cut(s)	339, 444, 472, 516, 690, 823, 993, 1465
HphI	GGTGA	6 cut(s)	247, 439, 502, 877, 910, 1321
Hpy166II	GTNNAC	6 cut(s)	145, 523, 810, 1201, 1210, 1429
Hpy188I	TCNGA	5 cut(s)	127, 647, 876, 932, 1401
Hpy188III	TCNNGA	2 cut(s)	820, 840
Hpy8I	GTNNAC	6 cut(s)	145, 523, 810, 1201, 1210, 1429
HpyAV	CCTTC	6 cut(s)	163, 322, 433, 514, 674, 992
HpyCH4III	ACNGT	1 cut(s)	1032
HpyCH4IV	ACGT	6 cut(s)	8, 261, 960, 969, 1197, 1425
HpyCH4V	TGCA	5 cut(s)	93, 190, 906, 1083, 1345
HpyF3I	CTNAG	4 cut(s)	474, 821, 1361, 1472
HpySE526I	ACGT	6 cut(s)	8, 261, 960, 969, 1197, 1425
Hsp92I	GRCGYC	3 cut(s)	108, 270, 969
Hsp92II	CATG	4 cut(s)	208, 244, 253, 1436
HspAI	GCGC	9 cut(s)	75, 213, 228, 741, 1130, 1152, 1175, 1314, 1448
Kpn2I	TCCGGA	1 cut(s)	839
KroI	GCCGGC	3 cut(s)	104, 215, 278
KroNI	GCCGGC	3 cut(s)	106, 217, 280
KspI	CCGCGG	1 cut(s)	733
Kzo9I	GATC	6 cut(s)	455, 816, 842, 1041, 1074, 1396
LmnI	GCTCC	2 cut(s)	224, 668
Lsp1109I	GCAGC	6 cut(s)	89, 202, 1144, 1251, 1329, 1344
LweI	GCATC	2 cut(s)	660, 893
MaeI	CTAG	2 cut(s)	20, 567
MaeII	ACGT	6 cut(s)	8, 261, 960, 969, 1197, 1425
MaeIII	GTNAC	4 cut(s)	883, 950, 961, 974
MalI	GATC	6 cut(s)	457, 818, 844, 1043, 1076, 1398
MbiI	CCGCTC	1 cut(s)	914
MboI	GATC	6 cut(s)	455, 816, 842, 1041, 1074, 1396
MboII	GAAGA	7 cut(s)	154, 172, 280, 292, 349, 919, 1454
MflI	RGATCY	4 cut(s)	455, 816, 842, 1396
MhlI	GDGCHC	3 cut(s)	63, 764, 1276
MluCI	AATT	4 cut(s)	939, 1106, 1164, 1296
MlyI	GAGTC	2 cut(s)	466, 832
MmeI	TCCRAC	5 cut(s)	150, 357, 405, 625, 854
MreI	CGCCGGCG	2 cut(s)	104, 278
MroI	TCCGGA	1 cut(s)	839
MroNI	GCCGGC	3 cut(s)	104, 215, 278
MseI	TTAA	2 cut(s)	575, 795
MspA1I	CMGCKG	5 cut(s)	453, 732, 866, 881, 1120
MspR9I	CCNGG	7 cut(s)	527, 622, 716, 801, 1214, 1215, 1405
MvnI	CGCG	6 cut(s)	228, 732, 998, 1154, 1316, 1448
NaeI	GCCGGC	3 cut(s)	106, 217, 280
NciI	CCSGG	7 cut(s)	527, 622, 716, 801, 1214, 1215, 1405
NdeI	CATATG	1 cut(s)	639
NdeII	GATC	6 cut(s)	455, 816, 842, 1041, 1074, 1396
NgoMIV	GCCGGC	3 cut(s)	104, 215, 278
NlaIII	CATG	4 cut(s)	208, 244, 253, 1436
NmeAIII	GCCGAG	6 cut(s)	8, 174, 490, 966, 1120, 1482
NmuCI	GTSAC	2 cut(s)	883, 950
PcsI	WCGNNNNNNNCGW	3 cut(s)	699, 966, 1325
PdiI	GCCGGC	3 cut(s)	106, 217, 280
PfeI	GAWTC	6 cut(s)	339, 444, 516, 690, 993, 1465
Pfl23II	CGTACG	1 cut(s)	828
PinAI	ACCGGT	2 cut(s)	386, 602
Ple19I	CGATCG	1 cut(s)	1044
PleI	GAGTC	2 cut(s)	466, 831
PpsI	GAGTC	2 cut(s)	466, 831
Ppu21I	YACGTR	2 cut(s)	1198, 1426
PpuMI	RGGWCCY	1 cut(s)	373
PshAI	GACNNNNGTC	1 cut(s)	881
Psp5II	RGGWCCY	1 cut(s)	373
PspFI	CCCAGC	2 cut(s)	561, 1267
PspLI	CGTACG	1 cut(s)	828
PspOMI	GGGCCC	2 cut(s)	59, 1272
PspPPI	RGGWCCY	1 cut(s)	373
PsrI	GAACNNNNNNTAC	2 cut(s)	1193, 1225
PsuI	RGATCY	4 cut(s)	455, 816, 842, 1396
PvuI	CGATCG	1 cut(s)	1044
PvuII	CAGCTG	1 cut(s)	866
RsaI	GTAC	5 cut(s)	788, 830, 1196, 1211, 1430
RsaNI	GTAC	5 cut(s)	787, 829, 1195, 1210, 1429
Rsr2I	CGGWCCG	1 cut(s)	876
RsrII	CGGWCCG	1 cut(s)	876
SacII	CCGCGG	1 cut(s)	733
SalI	GTCGAC	1 cut(s)	143
SaqAI	TTAA	2 cut(s)	575, 795
Sau3AI	GATC	6 cut(s)	455, 816, 842, 1041, 1074, 1396
SchI	GAGTC	2 cut(s)	466, 832
ScrFI	CCNGG	7 cut(s)	527, 622, 716, 801, 1214, 1215, 1405
SduI	GDGCHC	3 cut(s)	63, 764, 1276
SfaNI	GCATC	2 cut(s)	660, 893
SfcI	CTRYAG	1 cut(s)	397
Sfr303I	CCGCGG	1 cut(s)	733
SgrAI	CRCCGGYG	3 cut(s)	71, 104, 278
SgrBI	CCGCGG	1 cut(s)	733
SinI	GGWCC	3 cut(s)	373, 634, 876
SmaI	CCCGGG	1 cut(s)	1215
Sse9I	AATT	4 cut(s)	939, 1106, 1164, 1296
SspMI	CTAG	2 cut(s)	20, 567
StyD4I	CCNGG	7 cut(s)	525, 620, 714, 799, 1212, 1213, 1403
StyI	CCWWGG	1 cut(s)	52
TaaI	ACNGT	1 cut(s)	1032
TaiI	ACGT	6 cut(s)	11, 264, 963, 972, 1200, 1428
TaqI	TCGA	9 cut(s)	144, 267, 393, 555, 612, 678, 702, 1026, 1110
TasI	AATT	4 cut(s)	939, 1106, 1164, 1296
TfiI	GAWTC	6 cut(s)	339, 444, 516, 690, 993, 1465
Tru1I	TTAA	2 cut(s)	575, 795
Tru9I	TTAA	2 cut(s)	575, 795
TscAI	CASTG	3 cut(s)	422, 545, 959
TseFI	GTSAC	2 cut(s)	883, 950
TseI	GCWGC	6 cut(s)	77, 190, 1132, 1264, 1342, 1357
Tsp45I	GTSAC	2 cut(s)	883, 950
TspDTI	ATGAA	4 cut(s)	76, 257, 331, 664
TspGWI	ACGGA	5 cut(s)	211, 351, 816, 980, 1155
TspMI	CCCGGG	1 cut(s)	1213
TspRI	CASTG	3 cut(s)	422, 545, 959
VpaK11BI	GGWCC	3 cut(s)	373, 634, 876
XagI	CCTNNNNNAGG	1 cut(s)	368
XapI	RAATTY	2 cut(s)	939, 1106
XmaI	CCCGGG	1 cut(s)	1213
XmiI	GTMKAC	2 cut(s)	144, 522
XspI	CTAG	2 cut(s)	20, 567
ZraI	GACGTC	1 cut(s)	970

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.