Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0013127

Physical Location & Seq

Genomic Coordinates Forward (+)

22123159 .. 22126494

Gene Structure 3336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1226400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGCGTTGAGCATGACGCACCAGATCAGCGCGCTCTCCGGGACTCCGATTAGGACGACCGACGCCAGCTCATCATCCGTGGAATCGGTCAGCGCGTCGCCAATGTGGAAGTCTCCGGCGGCGGGTCTGACTTGCAAAATTCAGAAGCCGGAAAGGGTTGACGGGCTGTCGCCGCCGTTGAGTCCGTGCAGATCGCCGGTTCTCGGGTCGAGCCGGCCGGATCTGTCCATGGCGTGCAGGGCGTTCGCGACGGAGGCGGAATCGCCGGTTCTGGAGCACCAGGTGAGGGGTGCGCACGATAAGGGGACGGGAGTGCCGGTTTACGTGATGATGCCGTTGGACAGCGTGACGATGCACAACTCGGTGAATCGGAAGAAGGCGATGAACGCGAGTCTGCAGGCGCTTAAGAGCGCTGGGGTGGAGGGTGTGATGATGGACGTATGGTGGGGGTTGGTGGAGAGGGACGCGCCCGGCGCGTACAATTGGGGCGGGTACGCTGAGCTTCTCGAGATGGCGAGGAAGCACGGTCTCAAAGTACAGGCTGTGATGTCGTTTCATCAATGTGGCGGGAACGTCGGTGACTCCGTCACCATTCCTTTACCCAAATGGGTTGTGGAGGAGGTCGATAAAGATCGAGACCTTGCATACACTGATCAGTGGGGAAAGAGGAATTGCGAGTATTTATCGCTGGGCGCTGATACCCTCCCGGTCCTCAAGGGCCGCACGCCGGTCCAGTGCTACGCGGACTTGATGCGTGCCTTCAGAGACAACTTCAAACACCTTCTTGGTGACACCATTGTGGAAATCCAAGTCGGAATGGGTCCAGCAGGTGAGCTGCGTTACCCTTCGTATCCAGAATCGAATGGGACATGGCAATTTCCAGGAATTGGCGCCTTCCAATGTTTTGACAAGTATATGCTCAGCAGCTTGAAAGCTGCTGCTGAAGCTGCGGGTAAGCCAGAATGGGGTAGCACAGGCCCCACTGATGCAGGTCACTATAACAACTGGCCGGAAGATACCCCATTTTTCAGAAAAGAAGGTGGAGGCTGGAACACTACTTATGGTGAATTCTTCCTCGGCTGGTACTCTCAGATGCTCTTGGACCACGGTGAGAGGATACTTACCTCAGCTAAGTCCATCTTCGAGGAGGCCGGTGTGAAGATCTCAGTAAAGGTTGCAGGTATCCACTGGCACTACGGGACCCGGTCCCATGCCCCCGAGCTCACAGCAGGGTACTACAACACACGATTCAGAGATGGTTACATTCCTATTGCGCAAATGCTAGCACGCCATGGTGCCATCTTCAATTTCACTTGCGTTGAGATGCGCGACCACGAGCAGCCACAGGAAGCTCAATGTTTACCAGAGAAGCTTGTTAGGCAAGTGGCTATGGCTACACAGAAAGTAAACGTACCACTTGCCGGTGAAAATGCTCTGCCAAGGTACGATGATTACGCACACGAGCAGATCTTGGAAGCATCATCTTTGAACGTTGAGGGCAACACCCAAGGCAACCAGATGTGCGCGTTCACATACTTGAGGATGAACCCGCACCTGTTCCAGCCCGACAACTGGAGGCGTTTTGTGGCGTTTGTGAAGAAAATGAAGGAAAAGGGTAGCCACAAGTGCAGGGAAGAGGTGGAGAGGGAAGCGGAGCATTTCGTGCACATTTCAACACCTTTGGTGCAGGAGGCTGCTGTTGCTCTGATGCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016052 GO:0016160 GO:0016161 GO:0016787 GO:0016798 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_14

Protein Analysis

572

Amino Acids

63.36

Weight (kDa)

6.3

Isoelectric Point (pI)

35.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_14	PF01373	108 - 531	2.4e-171	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013127)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23920
fragaria_vesca	FvH4_4g05230
malus_domestica	MD12G1073900.v1.1 MD13G1226400.v1.1 MD16G1231500.v1.1
prunus_persica	Prupe.1G053800_v2.0.a1
pyrus_communis	pycom03g22190 pycom13g19950 pycom16g19370
rosa_chinensis	RchiOBHm_Chr4g0396151
rosa_laevigata	RLG00000009565
rosa_multiflora	Rmu_sc0006325.1_g000039
rosa_roxburghii	Rroxscaffold_5G00341000
rosa_rugosa	Rorug03G0351400
rosa_samantha	Rh4BG065100 Rh4CG071900 Rh4DG061900
rosa_wichuraiana	Rw4G005380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	818
Acc16I	TGCGCA	2 cut(s)	294, 1275
Acc36I	ACCTGC	3 cut(s)	818, 980, 1169
AccB1I	GGYRCC	2 cut(s)	890, 1295
AccB7I	CCANNNNNTGG	1 cut(s)	887
AccII	CGCG	9 cut(s)	32, 95, 248, 389, 467, 476, 743, 1329, 1526
AclI	AACGTT	1 cut(s)	1491
AclWI	GGATC	1 cut(s)	228
AcoI	YGGCCR	2 cut(s)	215, 1007
AcsI	RAATTY	2 cut(s)	138, 1067
AcuI	CTGAAG	2 cut(s)	745, 963
AcyI	GRCGYC	2 cut(s)	63, 891
AdeI	CACNNNGTG	1 cut(s)	283
AfaI	GTAC	7 cut(s)	479, 494, 537, 1085, 1235, 1413, 1445
AfeI	AGCGCT	1 cut(s)	412
AfiI	CCNNNNNNNGG	8 cut(s)	122, 203, 286, 727, 887, 965, 1421, 1572
AflII	CTTAAG	1 cut(s)	404
AgsI	TTSAA	5 cut(s)	775, 931, 1306, 1489, 1674
AhdI	GACNNNNNGTC	1 cut(s)	166
AjnI	CCWGG	2 cut(s)	279, 880
AleI	CACNNNNGTG	1 cut(s)	797
Alw21I	GWGCWC	3 cut(s)	279, 1224, 1668
Alw26I	GTCTC	4 cut(s)	117, 533, 630, 759
Alw44I	GTGCAC	1 cut(s)	1664
AlwI	GGATC	1 cut(s)	228
Ama87I	CYCGRG	3 cut(s)	203, 506, 1217
Aor51HI	AGCGCT	1 cut(s)	412
AoxI	GGCC	5 cut(s)	215, 718, 976, 1007, 1149
ApaLI	GTGCAC	1 cut(s)	1664
ApeKI	GCWGC	7 cut(s)	835, 924, 935, 938, 947, 1339, 1694
ApoI	RAATTY	2 cut(s)	138, 1067
AspS9I	GGNCC	8 cut(s)	709, 718, 730, 821, 977, 1102, 1200, 1206
AsuC2I	CCSGG	4 cut(s)	40, 471, 707, 1204
AsuHPI	GGTGA	9 cut(s)	295, 376, 580, 590, 800, 842, 1076, 1121, 1436
AsuNHI	GCTAGC	1 cut(s)	1282
AvaI	CYCGRG	3 cut(s)	203, 506, 1217
AvaII	GGWCC	6 cut(s)	709, 730, 821, 1102, 1200, 1206
BaeGI	GKGCMC	1 cut(s)	1668
BanI	GGYRCC	2 cut(s)	890, 1295
BanII	GRGCYC	1 cut(s)	1224
BauI	CACGAG	2 cut(s)	1334, 1460
Bbv12I	GWGCWC	3 cut(s)	279, 1224, 1668
BbvCI	CCTCAGC	1 cut(s)	1126
BbvI	GCAGC	7 cut(s)	822, 922, 925, 934, 936, 1351, 1681
BccI	CCATC	5 cut(s)	427, 505, 1145, 1250, 1307
BceAI	ACGGC	2 cut(s)	160, 319
BcgI	CGANNNNNNTGC	2 cut(s)	226, 260
BciT130I	CCWGG	2 cut(s)	281, 882
BciVI	GTATCC	3 cut(s)	861, 1110, 1193
BclI	TGATCA	1 cut(s)	652
BcnI	CCSGG	4 cut(s)	40, 471, 707, 1204
BcoDI	GTCTC	4 cut(s)	117, 533, 630, 759
BfaI	CTAG	1 cut(s)	1283
BfmI	CTRYAG	1 cut(s)	395
BfoI	RGCGCY	4 cut(s)	404, 414, 696, 894
BfrI	CTTAAG	1 cut(s)	404
BfuAI	ACCTGC	3 cut(s)	818, 980, 1169
BfuI	GTATCC	3 cut(s)	861, 1110, 1193
BglII	AGATCT	2 cut(s)	1161, 1467
BlpI	GCTNAGC	2 cut(s)	498, 920
Bme1390I	CCNGG	6 cut(s)	40, 281, 471, 707, 882, 1204
Bme18I	GGWCC	6 cut(s)	709, 730, 821, 1102, 1200, 1206
BmeRI	GACNNNNNGTC	1 cut(s)	166
BmeT110I	CYCGRG	3 cut(s)	203, 506, 1217
BmgT120I	GGNCC	8 cut(s)	709, 718, 730, 821, 977, 1102, 1200, 1206
BmiI	GGNNCC	7 cut(s)	822, 892, 979, 1201, 1202, 1208, 1297
BmrFI	CCNGG	6 cut(s)	40, 281, 471, 707, 882, 1204
BmsI	GCATC	8 cut(s)	321, 342, 741, 976, 1083, 1314, 1487, 1698
BmtI	GCTAGC	1 cut(s)	1286
BpmI	CTGGAG	2 cut(s)	293, 1594
Bpu10I	CCTNAGC	1 cut(s)	1126
Bpu1102I	GCTNAGC	2 cut(s)	498, 920
BpuEI	CTTGAG	2 cut(s)	698, 1558
BpuMI	CCSGG	4 cut(s)	40, 471, 707, 1204
BsaAI	YACGTR	1 cut(s)	325
BsaBI	GATNNNNATC	1 cut(s)	630
BsaHI	GRCGYC	2 cut(s)	63, 891
BsaI	GGTCTC	2 cut(s)	533, 630
BsaJI	CCNNGG	7 cut(s)	78, 228, 1075, 1105, 1291, 1439, 1507
BsaXI	ACNNNNNCTCC	2 cut(s)	303, 333
Bsc4I	CCNNNNNNNGG	8 cut(s)	122, 203, 286, 727, 887, 965, 1421, 1572
Bse118I	RCCGGY	7 cut(s)	196, 213, 265, 316, 727, 1151, 1421
Bse1I	ACTGG	4 cut(s)	733, 1010, 1193, 1577
Bse8I	GATNNNNATC	1 cut(s)	630
BseBI	CCWGG	2 cut(s)	281, 882
BseDI	CCNNGG	7 cut(s)	78, 228, 1075, 1105, 1291, 1439, 1507
BseGI	GGATG	2 cut(s)	74, 1548
BseJI	GATNNNNATC	1 cut(s)	630
BseLI	CCNNNNNNNGG	8 cut(s)	122, 203, 286, 727, 887, 965, 1421, 1572
BseMII	CTCAG	5 cut(s)	489, 934, 1103, 1140, 1179
BseNI	ACTGG	4 cut(s)	733, 1010, 1193, 1577
BsePI	GCGCGC	1 cut(s)	30
BseRI	GAGGAG	2 cut(s)	632, 1160
BseSI	GKGCMC	1 cut(s)	1668
BseX3I	CGGCCG	1 cut(s)	215
BseXI	GCAGC	7 cut(s)	822, 922, 925, 934, 936, 1351, 1681
BseYI	CCCAGC	2 cut(s)	413, 688
BsgI	GTGCAG	4 cut(s)	208, 256, 1648, 1706
Bsh1236I	CGCG	9 cut(s)	32, 95, 248, 389, 467, 476, 743, 1329, 1526
Bsh1285I	CGRYCG	2 cut(s)	60, 218
BshFI	GGCC	5 cut(s)	217, 720, 978, 1009, 1151
BshNI	GGYRCC	2 cut(s)	890, 1295
BsiEI	CGRYCG	2 cut(s)	60, 218
BsiHKAI	GWGCWC	3 cut(s)	279, 1224, 1668
BsiHKCI	CYCGRG	3 cut(s)	203, 506, 1217
BslFI	GGGAC	6 cut(s)	55, 319, 476, 880, 1192, 1213
BslI	CCNNNNNNNGG	8 cut(s)	122, 203, 286, 727, 887, 965, 1421, 1572
BsmAI	GTCTC	4 cut(s)	117, 533, 630, 759
BsmFI	GGGAC	6 cut(s)	55, 319, 476, 880, 1192, 1213
BsnI	GGCC	5 cut(s)	217, 720, 978, 1009, 1151
Bso31I	GGTCTC	2 cut(s)	533, 630
BsoBI	CYCGRG	3 cut(s)	203, 506, 1217
Bsp1286I	GDGCHC	3 cut(s)	279, 1224, 1668
Bsp143I	GATC	7 cut(s)	24, 191, 220, 631, 652, 1161, 1467
Bsp1720I	GCTNAGC	2 cut(s)	498, 920
Bsp19I	CCATGG	2 cut(s)	228, 1291
Bsp68I	TCGCGA	1 cut(s)	248
BspANI	GGCC	5 cut(s)	217, 720, 978, 1009, 1151
BspCNI	CTCAG	5 cut(s)	490, 933, 1102, 1139, 1178
BspFNI	CGCG	9 cut(s)	32, 95, 248, 389, 467, 476, 743, 1329, 1526
BspLI	GGNNCC	7 cut(s)	822, 892, 979, 1201, 1202, 1208, 1297
BspMAI	CTGCAG	1 cut(s)	399
BspMI	ACCTGC	3 cut(s)	818, 980, 1169
BspOI	GCTAGC	1 cut(s)	1286
BspPI	GGATC	1 cut(s)	228
BspT107I	GGYRCC	2 cut(s)	890, 1295
BspTI	CTTAAG	1 cut(s)	404
BspTNI	GGTCTC	2 cut(s)	533, 630
BsrFI	RCCGGY	7 cut(s)	196, 213, 265, 316, 727, 1151, 1421
BsrI	ACTGG	4 cut(s)	733, 1010, 1193, 1577
BssAI	RCCGGY	7 cut(s)	196, 213, 265, 316, 727, 1151, 1421
BssECI	CCNNGG	7 cut(s)	78, 228, 1075, 1105, 1291, 1439, 1507
BssHII	GCGCGC	1 cut(s)	30
BssMI	GATC	7 cut(s)	24, 191, 220, 631, 652, 1161, 1467
BssNI	GRCGYC	2 cut(s)	63, 891
BssSI	CACGAG	2 cut(s)	1334, 1460
BssT1I	CCWWGG	4 cut(s)	228, 1291, 1439, 1507
Bst2BI	CACGAG	2 cut(s)	1334, 1460
Bst2UI	CCWGG	2 cut(s)	281, 882
Bst4CI	ACNGT	2 cut(s)	527, 1109
Bst6I	CTCTTC	1 cut(s)	1629
BstACI	GRCGYC	2 cut(s)	63, 891
BstAFI	CTTAAG	1 cut(s)	404
BstAPI	GCANNNNNTGC	1 cut(s)	1663
BstBAI	YACGTR	1 cut(s)	325
BstC8I	GCNNGC	9 cut(s)	32, 67, 215, 235, 399, 725, 756, 1284, 1288
BstDEI	CTNAG	6 cut(s)	498, 920, 1089, 1126, 1131, 1165
BstDSI	CCRYGG	4 cut(s)	78, 228, 1105, 1291
BstF5I	GGATG	2 cut(s)	74, 1548
BstFNI	CGCG	9 cut(s)	32, 95, 248, 389, 467, 476, 743, 1329, 1526
BstH2I	RGCGCY	4 cut(s)	404, 414, 696, 894
BstKTI	GATC	7 cut(s)	27, 194, 223, 634, 655, 1164, 1470
BstMAI	GTCTC	4 cut(s)	117, 533, 630, 759
BstMBI	GATC	7 cut(s)	24, 191, 220, 631, 652, 1161, 1467
BstMCI	CGRYCG	2 cut(s)	60, 218
BstNI	CCWGG	2 cut(s)	281, 882
BstSCI	CCNGG	6 cut(s)	38, 279, 469, 705, 880, 1202
BstSFI	CTRYAG	1 cut(s)	395
BstSLI	GKGCMC	1 cut(s)	1668
BstUI	CGCG	9 cut(s)	32, 95, 248, 389, 467, 476, 743, 1329, 1526
BstV1I	GCAGC	7 cut(s)	822, 922, 925, 934, 936, 1351, 1681
BstX2I	RGATCY	3 cut(s)	220, 1161, 1467
BstYI	RGATCY	3 cut(s)	220, 1161, 1467
BstZI	CGGCCG	1 cut(s)	215
BsuI	GTATCC	3 cut(s)	861, 1110, 1193
BsuRI	GGCC	5 cut(s)	217, 720, 978, 1009, 1151
BtgI	CCRYGG	4 cut(s)	78, 228, 1105, 1291
BtgZI	GCGATG	1 cut(s)	395
BtsCI	GGATG	2 cut(s)	74, 1548
BtsIMutI	CAGTG	5 cut(s)	648, 662, 740, 981, 1186
BtuMI	TCGCGA	1 cut(s)	248
BveI	ACCTGC	3 cut(s)	818, 980, 1169
Cac8I	GCNNGC	9 cut(s)	32, 67, 215, 235, 399, 725, 756, 1284, 1288
Cfr10I	RCCGGY	7 cut(s)	196, 213, 265, 316, 727, 1151, 1421
Cfr13I	GGNCC	8 cut(s)	709, 718, 730, 821, 977, 1102, 1200, 1206
CseI	GACGC	4 cut(s)	25, 71, 84, 473
CsiI	ACCWGGT	1 cut(s)	279
Csp6I	GTAC	7 cut(s)	478, 493, 536, 1084, 1234, 1412, 1444
CviAII	CATG	5 cut(s)	13, 229, 870, 1211, 1292
CviQI	GTAC	7 cut(s)	478, 493, 536, 1084, 1234, 1412, 1444
DdeI	CTNAG	6 cut(s)	498, 920, 1089, 1126, 1131, 1165
DinI	GGCGCC	1 cut(s)	892
DpnI	GATC	7 cut(s)	26, 193, 222, 633, 654, 1163, 1469
DpnII	GATC	7 cut(s)	24, 191, 220, 631, 652, 1161, 1467
DraIII	CACNNNGTG	1 cut(s)	283
DriI	GACNNNNNGTC	1 cut(s)	166
EaeI	YGGCCR	2 cut(s)	215, 1007
EagI	CGGCCG	1 cut(s)	215
Eam1104I	CTCTTC	1 cut(s)	1629
Eam1105I	GACNNNNNGTC	1 cut(s)	166
EarI	CTCTTC	1 cut(s)	1629
EciI	GGCGGA	1 cut(s)	272
Ecl136II	GAGCTC	1 cut(s)	1222
EclXI	CGGCCG	1 cut(s)	215
Eco130I	CCWWGG	4 cut(s)	228, 1291, 1439, 1507
Eco24I	GRGCYC	1 cut(s)	1224
Eco31I	GGTCTC	2 cut(s)	533, 630
Eco47I	GGWCC	6 cut(s)	709, 730, 821, 1102, 1200, 1206
Eco47III	AGCGCT	1 cut(s)	412
Eco52I	CGGCCG	1 cut(s)	215
Eco53kI	GAGCTC	1 cut(s)	1222
Eco57I	CTGAAG	2 cut(s)	745, 963
Eco88I	CYCGRG	3 cut(s)	203, 506, 1217
EcoICRI	GAGCTC	1 cut(s)	1222
EcoO109I	RGGNCCY	2 cut(s)	977, 1200
EcoRI	GAATTC	1 cut(s)	1067
EcoRII	CCWGG	2 cut(s)	279, 880
EcoT14I	CCWWGG	4 cut(s)	228, 1291, 1439, 1507
EcoT38I	GRGCYC	1 cut(s)	1224
EgeI	GGCGCC	1 cut(s)	892
EheI	GGCGCC	1 cut(s)	892
ErhI	CCWWGG	4 cut(s)	228, 1291, 1439, 1507
FaeI	CATG	5 cut(s)	16, 232, 873, 1214, 1295
FalI	AAGNNNNNCTT	2 cut(s)	1124, 1156
FaqI	GGGAC	6 cut(s)	55, 319, 476, 880, 1192, 1213
FatI	CATG	5 cut(s)	12, 228, 869, 1210, 1291
FauI	CCCGC	5 cut(s)	115, 482, 560, 943, 1557
FbaI	TGATCA	1 cut(s)	652
FokI	GGATG	2 cut(s)	61, 1555
FriOI	GRGCYC	1 cut(s)	1224
FspAI	RTGCGCAY	1 cut(s)	294
FspBI	CTAG	1 cut(s)	1283
FspI	TGCGCA	2 cut(s)	294, 1275
GsaI	CCCAGC	2 cut(s)	417, 692
GsuI	CTGGAG	2 cut(s)	293, 1594
HaeII	RGCGCY	4 cut(s)	404, 414, 696, 894
HaeIII	GGCC	5 cut(s)	217, 720, 978, 1009, 1151
HgaI	GACGC	4 cut(s)	25, 71, 84, 473
Hin1I	GRCGYC	2 cut(s)	63, 891
Hin1II	CATG	5 cut(s)	16, 232, 873, 1214, 1295
HincII	GTYRAC	1 cut(s)	160
HindII	GTYRAC	1 cut(s)	160
HindIII	AAGCTT	1 cut(s)	1370
HinfI	GANTC	9 cut(s)	43, 83, 181, 260, 367, 391, 581, 857, 1248
HphI	GGTGA	9 cut(s)	295, 376, 580, 590, 800, 842, 1076, 1121, 1436
Hpy166II	GTNNAC	6 cut(s)	160, 322, 1361, 1408, 1530, 1666
Hpy188III	TCNNGA	6 cut(s)	247, 272, 506, 508, 635, 854
Hpy8I	GTNNAC	6 cut(s)	160, 322, 1361, 1408, 1530, 1666
Hpy99I	CGWCG	4 cut(s)	65, 100, 253, 578
HpyAV	CCTTC	7 cut(s)	370, 769, 791, 855, 904, 1031, 1600
HpyCH4III	ACNGT	2 cut(s)	527, 1109
HpyCH4IV	ACGT	5 cut(s)	324, 438, 573, 1410, 1491
HpyF3I	CTNAG	6 cut(s)	498, 920, 1089, 1126, 1131, 1165
HpySE526I	ACGT	5 cut(s)	324, 438, 573, 1410, 1491
Hsp92I	GRCGYC	2 cut(s)	63, 891
Hsp92II	CATG	5 cut(s)	16, 232, 873, 1214, 1295
KasI	GGCGCC	1 cut(s)	890
KflI	GGGWCCC	1 cut(s)	1200
KroI	GCCGGC	1 cut(s)	213
KroNI	GCCGGC	1 cut(s)	215
Ksp22I	TGATCA	1 cut(s)	652
Kzo9I	GATC	7 cut(s)	24, 191, 220, 631, 652, 1161, 1467
LmnI	GCTCC	2 cut(s)	274, 1654
Lsp1109I	GCAGC	7 cut(s)	822, 922, 925, 934, 936, 1351, 1681
LweI	GCATC	8 cut(s)	321, 342, 741, 976, 1083, 1314, 1487, 1698
MabI	ACCWGGT	1 cut(s)	279
MaeI	CTAG	1 cut(s)	1283
MaeII	ACGT	5 cut(s)	324, 438, 573, 1410, 1491
MaeIII	GTNAC	7 cut(s)	346, 578, 586, 788, 839, 992, 1259
MalI	GATC	7 cut(s)	26, 193, 222, 633, 654, 1163, 1469
MboI	GATC	7 cut(s)	24, 191, 220, 631, 652, 1161, 1467
MboII	GAAGA	8 cut(s)	385, 1025, 1063, 1132, 1171, 1294, 1609, 1646
MfeI	CAATTG	1 cut(s)	481
MflI	RGATCY	3 cut(s)	220, 1161, 1467
MhlI	GDGCHC	3 cut(s)	279, 1224, 1668
MluCI	AATT	7 cut(s)	138, 481, 670, 875, 885, 1067, 1306
Mly113I	GGCGCC	1 cut(s)	891
MlyI	GAGTC	4 cut(s)	37, 190, 400, 575
MmeI	TCCRAC	2 cut(s)	318, 793
MroNI	GCCGGC	1 cut(s)	213
MseI	TTAA	1 cut(s)	405
MslI	CAYNNNNRTG	2 cut(s)	561, 797
MspCI	CTTAAG	1 cut(s)	404
MspR9I	CCNGG	6 cut(s)	40, 281, 471, 707, 882, 1204
MunI	CAATTG	1 cut(s)	481
MvaI	CCWGG	2 cut(s)	281, 882
MvnI	CGCG	9 cut(s)	32, 95, 248, 389, 467, 476, 743, 1329, 1526
NaeI	GCCGGC	1 cut(s)	215
NarI	GGCGCC	1 cut(s)	891
NciI	CCSGG	4 cut(s)	40, 471, 707, 1204
NcoI	CCATGG	2 cut(s)	228, 1291
NdeII	GATC	7 cut(s)	24, 191, 220, 631, 652, 1161, 1467
NgoMIV	GCCGGC	1 cut(s)	213
NheI	GCTAGC	1 cut(s)	1282
NlaIII	CATG	5 cut(s)	16, 232, 873, 1214, 1295
NlaIV	GGNNCC	7 cut(s)	822, 892, 979, 1201, 1202, 1208, 1297
NmeAIII	GCCGAG	1 cut(s)	1056
NmuCI	GTSAC	5 cut(s)	346, 578, 586, 788, 992
NruI	TCGCGA	1 cut(s)	248
NsbI	TGCGCA	2 cut(s)	294, 1275
OliI	CACNNNNGTG	1 cut(s)	797
PaeR7I	CTCGAG	1 cut(s)	506
PaqCI	CACCTGC	1 cut(s)	818
PauI	GCGCGC	1 cut(s)	30
PcsI	WCGNNNNNNNCGW	2 cut(s)	92, 582
PdiI	GCCGGC	1 cut(s)	215
PfeI	GAWTC	5 cut(s)	83, 260, 367, 857, 1248
PflFI	GACNNNGTC	2 cut(s)	584, 1204
PflMI	CCANNNNNTGG	1 cut(s)	887
PfoI	TCCNGGA	2 cut(s)	38, 880
PleI	GAGTC	4 cut(s)	37, 189, 399, 575
PluTI	GGCGCC	1 cut(s)	894
PpsI	GAGTC	4 cut(s)	37, 189, 399, 575
Ppu21I	YACGTR	1 cut(s)	325
PpuMI	RGGWCCY	1 cut(s)	1200
Psp124BI	GAGCTC	1 cut(s)	1224
Psp1406I	AACGTT	1 cut(s)	1491
Psp5II	RGGWCCY	1 cut(s)	1200
Psp6I	CCWGG	2 cut(s)	279, 880
PspFI	CCCAGC	2 cut(s)	413, 688
PspGI	CCWGG	2 cut(s)	279, 880
PspN4I	GGNNCC	7 cut(s)	822, 892, 979, 1201, 1202, 1208, 1297
PspPI	GGNCC	8 cut(s)	709, 718, 730, 821, 977, 1102, 1200, 1206
PspPPI	RGGWCCY	1 cut(s)	1200
PstI	CTGCAG	1 cut(s)	399
PsuI	RGATCY	3 cut(s)	220, 1161, 1467
PsyI	GACNNNGTC	2 cut(s)	584, 1204
PteI	GCGCGC	1 cut(s)	30
RruI	TCGCGA	1 cut(s)	248
RsaI	GTAC	7 cut(s)	479, 494, 537, 1085, 1235, 1413, 1445
RsaNI	GTAC	7 cut(s)	478, 493, 536, 1084, 1234, 1412, 1444
RseI	CAYNNNNRTG	2 cut(s)	561, 797
SacI	GAGCTC	1 cut(s)	1224
SaqAI	TTAA	1 cut(s)	405
Sau3AI	GATC	7 cut(s)	24, 191, 220, 631, 652, 1161, 1467
Sau96I	GGNCC	8 cut(s)	709, 718, 730, 821, 977, 1102, 1200, 1206
SchI	GAGTC	4 cut(s)	37, 190, 400, 575
ScrFI	CCNGG	6 cut(s)	40, 281, 471, 707, 882, 1204
SduI	GDGCHC	3 cut(s)	279, 1224, 1668
SexAI	ACCWGGT	1 cut(s)	279
SfaNI	GCATC	8 cut(s)	321, 342, 741, 976, 1083, 1314, 1487, 1698
SfcI	CTRYAG	1 cut(s)	395
SfoI	GGCGCC	1 cut(s)	892
Sfr274I	CTCGAG	1 cut(s)	506
SinI	GGWCC	6 cut(s)	709, 730, 821, 1102, 1200, 1206
SlaI	CTCGAG	1 cut(s)	506
SmiMI	CAYNNNNRTG	2 cut(s)	561, 797
SmlI	CTYRAG	4 cut(s)	404, 506, 713, 1537
SmoI	CTYRAG	4 cut(s)	404, 506, 713, 1537
Sse9I	AATT	7 cut(s)	138, 481, 670, 875, 885, 1067, 1306
SspDI	GGCGCC	1 cut(s)	890
SspMI	CTAG	1 cut(s)	1283
SstI	GAGCTC	1 cut(s)	1224
StyD4I	CCNGG	6 cut(s)	38, 279, 469, 705, 880, 1202
StyI	CCWWGG	4 cut(s)	228, 1291, 1439, 1507
TaaI	ACNGT	2 cut(s)	527, 1109
TaiI	ACGT	5 cut(s)	327, 441, 576, 1413, 1494
TaqI	TCGA	6 cut(s)	209, 507, 624, 634, 860, 1143
TaqII	GACCGA	2 cut(s)	74, 76
TasI	AATT	7 cut(s)	138, 481, 670, 875, 885, 1067, 1306
TatI	WGTACW	1 cut(s)	535
TauI	GCSGC	3 cut(s)	122, 175, 723
TfiI	GAWTC	5 cut(s)	83, 260, 367, 857, 1248
Tru1I	TTAA	1 cut(s)	405
Tru9I	TTAA	1 cut(s)	405
TscAI	CASTG	5 cut(s)	655, 662, 740, 988, 1193
TseFI	GTSAC	5 cut(s)	346, 578, 586, 788, 992
TseI	GCWGC	7 cut(s)	835, 924, 935, 938, 947, 1339, 1694
Tsp45I	GTSAC	5 cut(s)	346, 578, 586, 788, 992
TspDTI	ATGAA	4 cut(s)	398, 545, 1559, 1619
TspGWI	ACGGA	4 cut(s)	67, 174, 266, 574
TspRI	CASTG	5 cut(s)	655, 662, 740, 988, 1193
Tth111I	GACNNNGTC	2 cut(s)	584, 1204
Van91I	CCANNNNNTGG	1 cut(s)	887
Vha464I	CTTAAG	1 cut(s)	404
VneI	GTGCAC	1 cut(s)	1664
VpaK11BI	GGWCC	6 cut(s)	709, 730, 821, 1102, 1200, 1206
XapI	RAATTY	2 cut(s)	138, 1067
XcmI	CCANNNNNNNNNTGG	1 cut(s)	610
XhoI	CTCGAG	1 cut(s)	506
XspI	CTAG	1 cut(s)	1283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.