Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0013127

Physical Location & Seq

Genomic Coordinates Forward (+)

23732829 .. 23736834

Gene Structure 4006 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1231500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGCGTTGAGCATGACGCATCAGATCGGCGCACTCTCCGGGACTCCGGTTACGGCGACCGAAGCCAGCTCATCATCCGTTGAATCGGTCAGCACGTCGCCGATGTGGAAGTCTCCGGCGGCTAGTCTGACTTGCAAAATTCAGAAGCCGGAGGGGATGGACGGGTTGTCGCCGCCGTTAAGCCCGTGCAGATCGCCGGGTCTCGGGTCGAGCCGGGCGGATCTGTCGATGGCGTGCAGGGCGTTCGCGACGGAGGTGGAATCGCCGGTTCTGGAGCACCAGGTGAGGGGTGCGCGCGATAAGGGCACGGGAGTGCCGGTTTATGTGATGATGCCGTTGGACAGCGTGACGATGAACAACTCGGTGAACCGGAAGAAGGCGATGAACGCGAGTCTTCAGGCGCTGAAGAGCGCGGGGGCGGAGGGTGTGATGATGGACGTGTGGTGGGGGTTGGTGGAGAGGGAAGTGCCCGGCGCGTACAATTGGGGCGGGTACGCTGAGCTTCTCGAGATGGCGAAGAAGCACGGCCTCAAAGTACAGGCTGTGATGTCGTTTCATCGGTGCGGCGGGAACGTCGGTGACTCCGTTACCATTCCTTTACCCAAGTGGGTTGTGGAAGAGGTTGAGAAAGATCCAGACCTTGCATACACTGATCAGTGGGGAAGGAAGAATTGCGAGTATTTATCGCTTGGCGCTGATACCCTCCCAGTCCTCAAGGGCCGCACGCCGGTCCAGTGCTACGCCGACTTCATGCGTGCCTTCCGGGACAACTTCAAACACCTTCTTGGTGACACCATTGTGGAAATCCAAGTTGGAATGGGTCCAGCAGGTGAGCTGCGTTACCCTTCGTATCCCGAATCCAATGGGACATGGAAATTTCCAGGAATTGGCGCCTTCCAATGTTTTGACAAGTACATACTCGGTGGCTTGAAAGCTGCTGCTGAAGCTGCGGGTAAACCAGAATGGGGTAGCACAGGTCCCACTGATGCCGGCCACTATAACAACTGGCCGGAAGATACCCCTTTTTTCAGAAAAGACGGTGGAGGGTGGAACAGCACTTACGGTGAATTCTTCCTCGGCTGGTACTCCCAAATGCTCTTGGACCATGGTGAGAGGATACTTACCTCAGCTAAGTCTATCTTCGAGGAGGCCGGTGTAAAGATCTCAGTAAAGGTTGCAGGCATCCACTGGCATTACGGGACCCGGTCCCATGCCCCCGAGCTCACAGCAGGGTATTACAACACACGATTCAGAGATGGTTATGTTCCTATTGCGCAAATGCTAGCACGCCATGGTGCCATCTTCAATTTCACTTGTGTTGAGATGCACGACCACGAGCAGCCACAGGATGCTCAATGTTTACCAGAGAAGCTTGTTAGGCAAGTGGCTATGGCCACACAGAAAACAAACGTACCACTTGCGGGTGAAAATGCACTGCCAAGGTACGACGACTATGCACATAAGCAGATATTGGAAGCATCATCTCTGAAAGTTGAGGGCAACACTGAAGGCAACCAAATGTGTGCGTTCACATACTTGAGGATGAACCCGCATTTGTTCCAGCCCGATAACTGGAGGCGTTTTGTGGGGTTTGTAAAGAAAATGAAGGAAAAGGGTAGCCACAAGTGCCGGGAAGAGGTGGAGAGGGAAGCGGAGCATTTTGTGCACGTTTCAACACCTTCGGTGCAGGAGGCTGCCGTTTCTCTTATGCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016052 GO:0016160 GO:0016161 GO:0016787 GO:0016798 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_14

Protein Analysis

572

Amino Acids

63.02

Weight (kDa)

6.36

Isoelectric Point (pI)

35.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_14	PF01373	108 - 531	1.3e-170	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013127)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23920
fragaria_vesca	FvH4_4g05230
malus_domestica	MD12G1073900.v1.1 MD13G1226400.v1.1 MD16G1231500.v1.1
prunus_persica	Prupe.1G053800_v2.0.a1
pyrus_communis	pycom03g22190 pycom13g19950 pycom16g19370
rosa_chinensis	RchiOBHm_Chr4g0396151
rosa_laevigata	RLG00000009565
rosa_multiflora	Rmu_sc0006325.1_g000039
rosa_roxburghii	Rroxscaffold_5G00341000
rosa_rugosa	Rorug03G0351400
rosa_samantha	Rh4BG065100 Rh4CG071900 Rh4DG061900
rosa_wichuraiana	Rw4G005380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	818
Acc16I	TGCGCA	1 cut(s)	1275
Acc36I	ACCTGC	1 cut(s)	818
AccB1I	GGYRCC	2 cut(s)	890, 1295
AccB7I	CCANNNNNTGG	1 cut(s)	887
AccII	CGCG	6 cut(s)	248, 295, 297, 389, 413, 476
AclWI	GGATC	2 cut(s)	228, 626
AcoI	YGGCCR	3 cut(s)	991, 1007, 1392
AcsI	RAATTY	3 cut(s)	138, 875, 1067
AcuI	CTGAAG	4 cut(s)	380, 425, 963, 1527
AcyI	GRCGYC	1 cut(s)	891
AdeI	CACNNNGTG	1 cut(s)	283
AfaI	GTAC	7 cut(s)	479, 494, 537, 914, 1085, 1413, 1445
AfiI	CCNNNNNNNGG	9 cut(s)	203, 286, 376, 727, 887, 965, 1421, 1572, 1636
AflIII	ACRYGT	1 cut(s)	438
AgsI	TTSAA	5 cut(s)	83, 775, 931, 1306, 1674
AhdI	GACNNNNNGTC	1 cut(s)	166
AjiI	CACGTC	2 cut(s)	96, 439
AjnI	CCWGG	2 cut(s)	279, 880
AjuI	GAANNNNNNNTTGG	2 cut(s)	795, 827
AleI	CACNNNNGTG	2 cut(s)	311, 797
AluBI	AGCT	8 cut(s)	69, 502, 835, 935, 947, 1130, 1222, 1372
AluI	AGCT	8 cut(s)	69, 502, 835, 935, 947, 1130, 1222, 1372
Alw21I	GWGCWC	3 cut(s)	279, 1224, 1668
Alw26I	GTCTC	2 cut(s)	117, 206
Alw44I	GTGCAC	1 cut(s)	1664
AlwI	GGATC	2 cut(s)	228, 626
AlwNI	CAGNNNCTG	1 cut(s)	403
Ama87I	CYCGRG	3 cut(s)	203, 506, 1217
AoxI	GGCC	6 cut(s)	526, 718, 991, 1007, 1149, 1392
ApaLI	GTGCAC	1 cut(s)	1664
ApeKI	GCWGC	6 cut(s)	835, 935, 938, 947, 1339, 1694
ApoI	RAATTY	3 cut(s)	138, 875, 1067
AspLEI	GCGC	9 cut(s)	32, 295, 297, 403, 413, 476, 695, 893, 1276
AspS9I	GGNCC	7 cut(s)	718, 730, 821, 977, 1102, 1200, 1206
AsuC2I	CCSGG	7 cut(s)	40, 198, 215, 471, 764, 1204, 1631
AsuHPI	GGTGA	8 cut(s)	295, 376, 590, 800, 842, 1076, 1121, 1436
AsuNHI	GCTAGC	1 cut(s)	1282
AvaI	CYCGRG	3 cut(s)	203, 506, 1217
AvaII	GGWCC	6 cut(s)	730, 821, 977, 1102, 1200, 1206
BaeGI	GKGCMC	3 cut(s)	308, 471, 1668
BaeI	ACNNNNGTAYC	2 cut(s)	1395, 1428
BalI	TGGCCA	1 cut(s)	1394
BanI	GGYRCC	2 cut(s)	890, 1295
BanII	GRGCYC	1 cut(s)	1224
BauI	CACGAG	1 cut(s)	1334
BbsI	GAAGAC	1 cut(s)	386
Bbv12I	GWGCWC	3 cut(s)	279, 1224, 1668
BbvCI	CCTCAGC	1 cut(s)	1126
BbvI	GCAGC	6 cut(s)	822, 922, 925, 934, 1351, 1681
BccI	CCATC	6 cut(s)	151, 223, 427, 505, 1250, 1307
BceAI	ACGGC	5 cut(s)	69, 160, 319, 541, 1682
BcgI	CGANNNNNNTGC	4 cut(s)	226, 260, 1436, 1470
BciT130I	CCWGG	2 cut(s)	281, 882
BciVI	GTATCC	2 cut(s)	861, 1110
BclI	TGATCA	1 cut(s)	652
BcnI	CCSGG	7 cut(s)	40, 198, 215, 471, 764, 1204, 1631
BcoDI	GTCTC	2 cut(s)	117, 206
BfaI	CTAG	2 cut(s)	123, 1283
BfoI	RGCGCY	3 cut(s)	404, 696, 894
BfuAI	ACCTGC	1 cut(s)	818
BfuI	GTATCC	2 cut(s)	861, 1110
BglII	AGATCT	1 cut(s)	1161
BlpI	GCTNAGC	1 cut(s)	498
Bme1390I	CCNGG	9 cut(s)	40, 198, 215, 281, 471, 764, 882, 1204, 1631
Bme18I	GGWCC	6 cut(s)	730, 821, 977, 1102, 1200, 1206
BmeRI	GACNNNNNGTC	1 cut(s)	166
BmeT110I	CYCGRG	3 cut(s)	203, 506, 1217
BmgBI	CACGTC	2 cut(s)	96, 439
BmgT120I	GGNCC	7 cut(s)	718, 730, 821, 977, 1102, 1200, 1206
BmiI	GGNNCC	7 cut(s)	822, 892, 979, 1201, 1202, 1208, 1297
BmrFI	CCNGG	9 cut(s)	40, 198, 215, 281, 471, 764, 882, 1204, 1631
BmrI	ACTGGG	1 cut(s)	701
BmsI	GCATC	7 cut(s)	28, 321, 976, 1191, 1314, 1339, 1487
BmtI	GCTAGC	1 cut(s)	1286
BmuI	ACTGGG	1 cut(s)	701
BpiI	GAAGAC	1 cut(s)	386
BpmI	CTGGAG	2 cut(s)	293, 1594
Bpu10I	CCTNAGC	1 cut(s)	1126
Bpu1102I	GCTNAGC	1 cut(s)	498
BpuEI	CTTGAG	2 cut(s)	698, 1558
BpuMI	CCSGG	7 cut(s)	40, 198, 215, 471, 764, 1204, 1631
BsaHI	GRCGYC	1 cut(s)	891
BsaI	GGTCTC	1 cut(s)	206
BsaJI	CCNNGG	4 cut(s)	1075, 1105, 1291, 1439
BsaWI	WCCGGW	2 cut(s)	46, 369
BsaXI	ACNNNNNCTCC	2 cut(s)	303, 333
Bsc4I	CCNNNNNNNGG	9 cut(s)	203, 286, 376, 727, 887, 965, 1421, 1572, 1636
Bse118I	RCCGGY	5 cut(s)	265, 316, 727, 989, 1151
Bse1I	ACTGG	5 cut(s)	707, 733, 1010, 1193, 1577
BseBI	CCWGG	2 cut(s)	281, 882
BseDI	CCNNGG	4 cut(s)	1075, 1105, 1291, 1439
BseGI	GGATG	5 cut(s)	74, 162, 1182, 1354, 1548
BseLI	CCNNNNNNNGG	9 cut(s)	203, 286, 376, 727, 887, 965, 1421, 1572, 1636
BseMII	CTCAG	3 cut(s)	489, 1140, 1179
BseNI	ACTGG	5 cut(s)	707, 733, 1010, 1193, 1577
BsePI	GCGCGC	1 cut(s)	293
BseRI	GAGGAG	1 cut(s)	1160
BseSI	GKGCMC	3 cut(s)	308, 471, 1668
BseXI	GCAGC	6 cut(s)	822, 922, 925, 934, 1351, 1681
BsgI	GTGCAG	3 cut(s)	208, 256, 1706
Bsh1236I	CGCG	6 cut(s)	248, 295, 297, 389, 413, 476
Bsh1285I	CGRYCG	1 cut(s)	60
BshFI	GGCC	6 cut(s)	528, 720, 993, 1009, 1151, 1394
BshNI	GGYRCC	2 cut(s)	890, 1295
BsiEI	CGRYCG	1 cut(s)	60
BsiHKAI	GWGCWC	3 cut(s)	279, 1224, 1668
BsiHKCI	CYCGRG	3 cut(s)	203, 506, 1217
BslFI	GGGAC	6 cut(s)	55, 779, 880, 963, 1192, 1213
BslI	CCNNNNNNNGG	9 cut(s)	203, 286, 376, 727, 887, 965, 1421, 1572, 1636
BsmAI	GTCTC	2 cut(s)	117, 206
BsmFI	GGGAC	6 cut(s)	55, 779, 880, 963, 1192, 1213
BsnI	GGCC	6 cut(s)	528, 720, 993, 1009, 1151, 1394
Bso31I	GGTCTC	1 cut(s)	206
BsoBI	CYCGRG	3 cut(s)	203, 506, 1217
Bsp1286I	GDGCHC	5 cut(s)	279, 308, 471, 1224, 1668
Bsp143I	GATC	6 cut(s)	24, 191, 220, 631, 652, 1161
Bsp1720I	GCTNAGC	1 cut(s)	498
Bsp19I	CCATGG	2 cut(s)	1105, 1291
Bsp68I	TCGCGA	1 cut(s)	248
BspANI	GGCC	6 cut(s)	528, 720, 993, 1009, 1151, 1394
BspCNI	CTCAG	3 cut(s)	490, 1139, 1178
BspFNI	CGCG	6 cut(s)	248, 295, 297, 389, 413, 476
BspLI	GGNNCC	7 cut(s)	822, 892, 979, 1201, 1202, 1208, 1297
BspMI	ACCTGC	1 cut(s)	818
BspOI	GCTAGC	1 cut(s)	1286
BspPI	GGATC	2 cut(s)	228, 626
BspQI	GCTCTTC	1 cut(s)	401
BspT107I	GGYRCC	2 cut(s)	890, 1295
BspTNI	GGTCTC	1 cut(s)	206
BsrFI	RCCGGY	5 cut(s)	265, 316, 727, 989, 1151
BsrI	ACTGG	5 cut(s)	707, 733, 1010, 1193, 1577
BssAI	RCCGGY	5 cut(s)	265, 316, 727, 989, 1151
BssECI	CCNNGG	4 cut(s)	1075, 1105, 1291, 1439
BssHII	GCGCGC	1 cut(s)	293
BssMI	GATC	6 cut(s)	24, 191, 220, 631, 652, 1161
BssNI	GRCGYC	1 cut(s)	891
BssSI	CACGAG	1 cut(s)	1334
BssT1I	CCWWGG	3 cut(s)	1105, 1291, 1439
Bst2BI	CACGAG	1 cut(s)	1334
Bst2UI	CCWGG	2 cut(s)	281, 882
Bst4CI	ACNGT	2 cut(s)	1040, 1064
Bst6I	CTCTTC	3 cut(s)	401, 612, 1629
BstACI	GRCGYC	1 cut(s)	891
BstAPI	GCANNNNNTGC	1 cut(s)	1663
BstC8I	GCNNGC	9 cut(s)	67, 235, 295, 725, 756, 991, 1180, 1284, 1288
BstDEI	CTNAG	4 cut(s)	498, 1126, 1131, 1165
BstDSI	CCRYGG	2 cut(s)	1105, 1291
BstF5I	GGATG	5 cut(s)	74, 162, 1182, 1354, 1548
BstFNI	CGCG	6 cut(s)	248, 295, 297, 389, 413, 476
BstH2I	RGCGCY	3 cut(s)	404, 696, 894
BstHHI	GCGC	9 cut(s)	32, 295, 297, 403, 413, 476, 695, 893, 1276
BstKTI	GATC	6 cut(s)	27, 194, 223, 634, 655, 1164
BstMAI	GTCTC	2 cut(s)	117, 206
BstMBI	GATC	6 cut(s)	24, 191, 220, 631, 652, 1161
BstMCI	CGRYCG	1 cut(s)	60
BstMWI	GCNNNNNNNGC	9 cut(s)	62, 239, 303, 386, 944, 947, 1378, 1626, 1663
BstNI	CCWGG	2 cut(s)	281, 882
BstSCI	CCNGG	9 cut(s)	38, 196, 213, 279, 469, 762, 880, 1202, 1629
BstSLI	GKGCMC	3 cut(s)	308, 471, 1668
BstUI	CGCG	6 cut(s)	248, 295, 297, 389, 413, 476
BstV1I	GCAGC	6 cut(s)	822, 922, 925, 934, 1351, 1681
BstV2I	GAAGAC	1 cut(s)	386
BstX2I	RGATCY	3 cut(s)	220, 631, 1161
BstYI	RGATCY	3 cut(s)	220, 631, 1161
BsuI	GTATCC	2 cut(s)	861, 1110
BsuRI	GGCC	6 cut(s)	528, 720, 993, 1009, 1151, 1394
BtgI	CCRYGG	2 cut(s)	1105, 1291
BtgZI	GCGATG	1 cut(s)	395
BtrI	CACGTC	2 cut(s)	96, 439
BtsCI	GGATG	5 cut(s)	74, 162, 1182, 1354, 1548
BtsI	GCAGTG	1 cut(s)	1433
BtsIMutI	CAGTG	7 cut(s)	648, 662, 740, 981, 1186, 1433, 1503
BtuMI	TCGCGA	1 cut(s)	248
BveI	ACCTGC	1 cut(s)	818
Cac8I	GCNNGC	9 cut(s)	67, 235, 295, 725, 756, 991, 1180, 1284, 1288
CaiI	CAGNNNCTG	1 cut(s)	403
CfoI	GCGC	9 cut(s)	32, 295, 297, 403, 413, 476, 695, 893, 1276
Cfr10I	RCCGGY	5 cut(s)	265, 316, 727, 989, 1151
Cfr13I	GGNCC	7 cut(s)	718, 730, 821, 977, 1102, 1200, 1206
CseI	GACGC	1 cut(s)	25
CsiI	ACCWGGT	1 cut(s)	279
Csp6I	GTAC	7 cut(s)	478, 493, 536, 913, 1084, 1412, 1444
CviAII	CATG	6 cut(s)	13, 751, 870, 1106, 1211, 1292
CviQI	GTAC	7 cut(s)	478, 493, 536, 913, 1084, 1412, 1444
DdeI	CTNAG	4 cut(s)	498, 1126, 1131, 1165
DinI	GGCGCC	1 cut(s)	892
DpnI	GATC	6 cut(s)	26, 193, 222, 633, 654, 1163
DpnII	GATC	6 cut(s)	24, 191, 220, 631, 652, 1161
DraIII	CACNNNGTG	1 cut(s)	283
DriI	GACNNNNNGTC	1 cut(s)	166
EaeI	YGGCCR	3 cut(s)	991, 1007, 1392
Eam1104I	CTCTTC	3 cut(s)	401, 612, 1629
Eam1105I	GACNNNNNGTC	1 cut(s)	166
EarI	CTCTTC	3 cut(s)	401, 612, 1629
EciI	GGCGGA	2 cut(s)	233, 434
Ecl136II	GAGCTC	1 cut(s)	1222
Eco130I	CCWWGG	3 cut(s)	1105, 1291, 1439
Eco24I	GRGCYC	1 cut(s)	1224
Eco31I	GGTCTC	1 cut(s)	206
Eco47I	GGWCC	6 cut(s)	730, 821, 977, 1102, 1200, 1206
Eco53kI	GAGCTC	1 cut(s)	1222
Eco57I	CTGAAG	4 cut(s)	380, 425, 963, 1527
Eco88I	CYCGRG	3 cut(s)	203, 506, 1217
EcoICRI	GAGCTC	1 cut(s)	1222
EcoO109I	RGGNCCY	2 cut(s)	977, 1200
EcoRI	GAATTC	1 cut(s)	1067
EcoRII	CCWGG	2 cut(s)	279, 880
EcoT14I	CCWWGG	3 cut(s)	1105, 1291, 1439
EcoT38I	GRGCYC	1 cut(s)	1224
EgeI	GGCGCC	1 cut(s)	892
EheI	GGCGCC	1 cut(s)	892
ErhI	CCWWGG	3 cut(s)	1105, 1291, 1439
FaeI	CATG	6 cut(s)	16, 754, 873, 1109, 1214, 1295
FalI	AAGNNNNNCTT	2 cut(s)	1124, 1156
FaqI	GGGAC	6 cut(s)	55, 779, 880, 963, 1192, 1213
FatI	CATG	6 cut(s)	12, 750, 869, 1105, 1210, 1291
FauI	CCCGC	6 cut(s)	406, 482, 560, 943, 1414, 1557
FbaI	TGATCA	1 cut(s)	652
FokI	GGATG	5 cut(s)	61, 169, 1169, 1361, 1555
FriOI	GRGCYC	1 cut(s)	1224
FspBI	CTAG	2 cut(s)	123, 1283
FspI	TGCGCA	1 cut(s)	1275
GlaI	GCGC	9 cut(s)	31, 294, 296, 402, 412, 475, 694, 892, 1275
GsuI	CTGGAG	2 cut(s)	293, 1594
HaeII	RGCGCY	3 cut(s)	404, 696, 894
HaeIII	GGCC	6 cut(s)	528, 720, 993, 1009, 1151, 1394
HgaI	GACGC	1 cut(s)	25
HhaI	GCGC	9 cut(s)	32, 295, 297, 403, 413, 476, 695, 893, 1276
Hin1I	GRCGYC	1 cut(s)	891
Hin1II	CATG	6 cut(s)	16, 754, 873, 1109, 1214, 1295
Hin6I	GCGC	9 cut(s)	30, 293, 295, 401, 411, 474, 693, 891, 1274
HinP1I	GCGC	9 cut(s)	30, 293, 295, 401, 411, 474, 693, 891, 1274
HindIII	AAGCTT	1 cut(s)	1370
HinfI	GANTC	7 cut(s)	43, 83, 260, 391, 581, 857, 1248
HphI	GGTGA	8 cut(s)	295, 376, 590, 800, 842, 1076, 1121, 1436
Hpy166II	GTNNAC	5 cut(s)	367, 956, 1361, 1530, 1666
Hpy188I	TCNGA	6 cut(s)	24, 129, 144, 1031, 1253, 1488
Hpy188III	TCNNGA	6 cut(s)	247, 272, 506, 508, 635, 854
Hpy8I	GTNNAC	5 cut(s)	367, 956, 1361, 1530, 1666
Hpy99I	CGWCG	4 cut(s)	100, 253, 578, 1451
HpyAV	CCTTC	9 cut(s)	370, 657, 769, 791, 855, 904, 1502, 1600, 1689
HpyCH4III	ACNGT	2 cut(s)	1040, 1064
HpyCH4IV	ACGT	5 cut(s)	95, 438, 573, 1410, 1668
HpyF10VI	GCNNNNNNNGC	9 cut(s)	62, 239, 303, 386, 944, 947, 1378, 1626, 1663
HpyF3I	CTNAG	4 cut(s)	498, 1126, 1131, 1165
HpySE526I	ACGT	5 cut(s)	95, 438, 573, 1410, 1668
Hsp92I	GRCGYC	1 cut(s)	891
Hsp92II	CATG	6 cut(s)	16, 754, 873, 1109, 1214, 1295
HspAI	GCGC	9 cut(s)	30, 293, 295, 401, 411, 474, 693, 891, 1274
KasI	GGCGCC	1 cut(s)	890
KflI	GGGWCCC	1 cut(s)	1200
KroI	GCCGGC	1 cut(s)	989
KroNI	GCCGGC	1 cut(s)	991
Ksp22I	TGATCA	1 cut(s)	652
Kzo9I	GATC	6 cut(s)	24, 191, 220, 631, 652, 1161
LguI	GCTCTTC	1 cut(s)	401
LmnI	GCTCC	2 cut(s)	274, 1654
Lsp1109I	GCAGC	6 cut(s)	822, 922, 925, 934, 1351, 1681
LweI	GCATC	7 cut(s)	28, 321, 976, 1191, 1314, 1339, 1487
MabI	ACCWGGT	1 cut(s)	279
MaeI	CTAG	2 cut(s)	123, 1283
MaeII	ACGT	5 cut(s)	95, 438, 573, 1410, 1668
MaeIII	GTNAC	6 cut(s)	49, 346, 578, 586, 788, 839
MalI	GATC	6 cut(s)	26, 193, 222, 633, 654, 1163
MboI	GATC	6 cut(s)	24, 191, 220, 631, 652, 1161
MfeI	CAATTG	1 cut(s)	481
MflI	RGATCY	3 cut(s)	220, 631, 1161
MhlI	GDGCHC	5 cut(s)	279, 308, 471, 1224, 1668
MlsI	TGGCCA	1 cut(s)	1394
MluCI	AATT	7 cut(s)	138, 481, 670, 875, 885, 1067, 1306
MluNI	TGGCCA	1 cut(s)	1394
Mly113I	GGCGCC	1 cut(s)	891
MlyI	GAGTC	3 cut(s)	37, 400, 575
MmeI	TCCRAC	2 cut(s)	318, 793
Mox20I	TGGCCA	1 cut(s)	1394
MroNI	GCCGGC	1 cut(s)	989
MscI	TGGCCA	1 cut(s)	1394
MseI	TTAA	1 cut(s)	179
MslI	CAYNNNNRTG	2 cut(s)	311, 797
Msp20I	TGGCCA	1 cut(s)	1394
MspR9I	CCNGG	9 cut(s)	40, 198, 215, 281, 471, 764, 882, 1204, 1631
MunI	CAATTG	1 cut(s)	481
MvaI	CCWGG	2 cut(s)	281, 882
MvnI	CGCG	6 cut(s)	248, 295, 297, 389, 413, 476
MwoI	GCNNNNNNNGC	9 cut(s)	62, 239, 303, 386, 944, 947, 1378, 1626, 1663
NaeI	GCCGGC	1 cut(s)	991
NarI	GGCGCC	1 cut(s)	891
NciI	CCSGG	7 cut(s)	40, 198, 215, 471, 764, 1204, 1631
NcoI	CCATGG	2 cut(s)	1105, 1291
NdeII	GATC	6 cut(s)	24, 191, 220, 631, 652, 1161
NgoMIV	GCCGGC	1 cut(s)	989
NheI	GCTAGC	1 cut(s)	1282
NlaIII	CATG	6 cut(s)	16, 754, 873, 1109, 1214, 1295
NlaIV	GGNNCC	7 cut(s)	822, 892, 979, 1201, 1202, 1208, 1297
NmeAIII	GCCGAG	1 cut(s)	1056
NmuCI	GTSAC	3 cut(s)	346, 578, 788
NruI	TCGCGA	1 cut(s)	248
NsbI	TGCGCA	1 cut(s)	1275
OliI	CACNNNNGTG	2 cut(s)	311, 797
PaeR7I	CTCGAG	1 cut(s)	506
PaqCI	CACCTGC	1 cut(s)	818
PauI	GCGCGC	1 cut(s)	293
PciSI	GCTCTTC	1 cut(s)	401
PcsI	WCGNNNNNNNCGW	2 cut(s)	92, 582
PdiI	GCCGGC	1 cut(s)	991
PfeI	GAWTC	4 cut(s)	83, 260, 857, 1248
PflFI	GACNNNGTC	1 cut(s)	1204
PflMI	CCANNNNNTGG	1 cut(s)	887
PfoI	TCCNGGA	3 cut(s)	38, 762, 880
PleI	GAGTC	3 cut(s)	37, 399, 575
PluTI	GGCGCC	1 cut(s)	894
PpsI	GAGTC	3 cut(s)	37, 399, 575
PpuMI	RGGWCCY	2 cut(s)	977, 1200
Psp124BI	GAGCTC	1 cut(s)	1224
Psp5II	RGGWCCY	2 cut(s)	977, 1200
Psp6I	CCWGG	2 cut(s)	279, 880
PspGI	CCWGG	2 cut(s)	279, 880
PspN4I	GGNNCC	7 cut(s)	822, 892, 979, 1201, 1202, 1208, 1297
PspPI	GGNCC	7 cut(s)	718, 730, 821, 977, 1102, 1200, 1206
PspPPI	RGGWCCY	2 cut(s)	977, 1200
PsrI	GAACNNNNNNTAC	2 cut(s)	1043, 1075
PstNI	CAGNNNCTG	1 cut(s)	403
PsuI	RGATCY	3 cut(s)	220, 631, 1161
PsyI	GACNNNGTC	1 cut(s)	1204
PteI	GCGCGC	1 cut(s)	293
RruI	TCGCGA	1 cut(s)	248
RsaI	GTAC	7 cut(s)	479, 494, 537, 914, 1085, 1413, 1445
RsaNI	GTAC	7 cut(s)	478, 493, 536, 913, 1084, 1412, 1444
RseI	CAYNNNNRTG	2 cut(s)	311, 797
SacI	GAGCTC	1 cut(s)	1224
SapI	GCTCTTC	1 cut(s)	401
SaqAI	TTAA	1 cut(s)	179
Sau3AI	GATC	6 cut(s)	24, 191, 220, 631, 652, 1161
Sau96I	GGNCC	7 cut(s)	718, 730, 821, 977, 1102, 1200, 1206
SchI	GAGTC	3 cut(s)	37, 400, 575
ScrFI	CCNGG	9 cut(s)	40, 198, 215, 281, 471, 764, 882, 1204, 1631
SduI	GDGCHC	5 cut(s)	279, 308, 471, 1224, 1668
SexAI	ACCWGGT	1 cut(s)	279
SfaNI	GCATC	7 cut(s)	28, 321, 976, 1191, 1314, 1339, 1487
SfoI	GGCGCC	1 cut(s)	892
Sfr274I	CTCGAG	1 cut(s)	506
SinI	GGWCC	6 cut(s)	730, 821, 977, 1102, 1200, 1206
SlaI	CTCGAG	1 cut(s)	506
SmiMI	CAYNNNNRTG	2 cut(s)	311, 797
SmlI	CTYRAG	3 cut(s)	506, 713, 1537
SmoI	CTYRAG	3 cut(s)	506, 713, 1537
Sse9I	AATT	7 cut(s)	138, 481, 670, 875, 885, 1067, 1306
SspDI	GGCGCC	1 cut(s)	890
SspMI	CTAG	2 cut(s)	123, 1283
SstI	GAGCTC	1 cut(s)	1224
StyD4I	CCNGG	9 cut(s)	38, 196, 213, 279, 469, 762, 880, 1202, 1629
StyI	CCWWGG	3 cut(s)	1105, 1291, 1439
TaaI	ACNGT	2 cut(s)	1040, 1064
TaiI	ACGT	5 cut(s)	98, 441, 576, 1413, 1671
TaqI	TCGA	4 cut(s)	209, 227, 507, 1143
TaqII	GACCGA	2 cut(s)	74, 76
TasI	AATT	7 cut(s)	138, 481, 670, 875, 885, 1067, 1306
TatI	WGTACW	2 cut(s)	535, 912
TauI	GCSGC	4 cut(s)	122, 175, 567, 723
TfiI	GAWTC	4 cut(s)	83, 260, 857, 1248
Tru1I	TTAA	1 cut(s)	179
Tru9I	TTAA	1 cut(s)	179
TscAI	CASTG	7 cut(s)	655, 662, 740, 988, 1193, 1440, 1510
TseFI	GTSAC	3 cut(s)	346, 578, 788
TseI	GCWGC	6 cut(s)	835, 935, 938, 947, 1339, 1694
Tsp45I	GTSAC	3 cut(s)	346, 578, 788
TspDTI	ATGAA	6 cut(s)	368, 398, 545, 739, 1559, 1619
TspGWI	ACGGA	3 cut(s)	67, 266, 574
TspRI	CASTG	7 cut(s)	655, 662, 740, 988, 1193, 1440, 1510
Tth111I	GACNNNGTC	1 cut(s)	1204
Van91I	CCANNNNNTGG	1 cut(s)	887
VneI	GTGCAC	1 cut(s)	1664
VpaK11BI	GGWCC	6 cut(s)	730, 821, 977, 1102, 1200, 1206
XapI	RAATTY	3 cut(s)	138, 875, 1067
XcmI	CCANNNNNNNNNTGG	1 cut(s)	610
XhoI	CTCGAG	1 cut(s)	506
XspI	CTAG	2 cut(s)	123, 1283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.