Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0013127

Physical Location & Seq

Genomic Coordinates Forward (+)

9481184 .. 9485081

Gene Structure 3898 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00341000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2025 bp

ATGGAGGGAATGCATGGCCCACCACCCCAATCCAGCCTTATCCCACGGCCGACACTTACCGGAAATCTTACGTGTTACTTCCCTTCGTGGACGCCGAAGCTGCCTCAGAAAGCAAACAAACAAAAACCATCACAAGCCGGACCTCCCCCACCTTTTACATGCCTTAAGCCGAAAGGTAGGACTACAAACCAGTCCCTCCCCGCCACGTGCCGCCCAAACGGGCCATCCCCGGGACTTCCAGATCCCACCGTCCGCCGGTCACGCGCGTCGAGCAATCGTAGCCGTCGCATGATCATCCGCATGGAGAGACGCCACCATCACACACCAGATCGGAGCACTGGCCGGGACGCCGATATCGGTGACGGAGCCGACCACGACCTCCGGCGAATCGACGGCGAGCGTGAGCGCTTCCGCGGTGAGGAAGTCACCGGGGACGAGCCTGACGTGCAAAATTCAGAGGCCGGAGGCGAAGGACGGGATGCTAACCCCGCCGTTGAGCCCGTGCCGGTCGCCGGTTCTCGGGGCGACCCGGCCGGATCTGTCGGTGGCGTGCCAGGCGTACGCGGAGGCTCCGGTTTTGGAGCACGAGGCGAGGGCCGCCGGGGAGAGGGTAAACGGCGTGCCGGTTTTTGTAATGATGCCGTTGGACAGCGTGACGATGAACCACACGGTGAATCGGAAGAAGGCGATGAACGCGAGTCTTCAGGCGTTGAAGAGCGCAGGGGTGGAGGGGATCATGATGGACGTGTGGTGGGGTTGGTGGAGAGGGACGCGCCCGGCGCGTACAATTGGGGCGGTTACGCCGAACTCTTGGAGATGGCCAAGAAACACGGCCTCAAAGTTCAGGCCGTCATGTCGTTCCACAAGTGCGGCGGTAACGTCGGGGACTCTTGCACTATTCCTTTACCCAGTTGGGTTGTGGAGGAGGTTAACAACGATCCAGACCTTGCGTACACTGATCAATGGGGAAATAGGAACTACGAGTATCTTTCACTTGGCGCTGATACACTTCCGGTCCTTAAAGGCCGCACGCCAGTGCAATGTTACGCTGACTTCATGCGTGCCTTCAGAGATAACTTCAAACACCTTCTTGGTGACACCATTGTGGAAATCCAAGTTGGGATGGGTCCAGCAGGTGAGCTCCGTTACCCTTCATATCCAGAGGGGGATGGGAGATGGTCATTTCCTGGAATTGGTGCCTTCCAATGTTTTGACAAGTATATGCTGAGTAGCTTAAAAGCTTATTGCGAATCTGAGGGTAAGCCAGAATGGGGTAGCACAGGCCCCACTGATGCCGGTCACTACAATAACTGGCCGGAAGATACCTCCTTCTTCAGAAGAGAAGGTGGAGGCTGGAATAGTAGTTACGGTGAATTCTTCCTAGGCTGGTACTCCCAGTTGCTCATGGACCATGGTGAGAGGATCCTTTCCTCAGCCAAGTCTATCTATGAGAATGAGGGCGTGAAAATCTCAGTAAAGATTGCAGGCATCCATTGGCATTATGGAACCCGGTCCCACGCCCCCGAGCTCACAGCAGGGTACTACAACACACGATTCCGGGATGGTTACCTACCCCTTGCTCAGATGTTAGCCCGCCATGGTGCCATTTTCAATTTCACTTGCATTGAGATGCACGACCACGAGCAGCCACAGGAAGCCCAATGTTCCCCTGAGAAGCTTGTTAGGCAAGTGGCCTTGGCCACTCAGAAGGCAGGAGTACCACTTGCAGGTGAAAATGCATTGCCAAGGTATGATGACTATGCACACGAGCAGATCTTGCAAGCATCAGCGTTGAACATTGAGGGCAACACTGAAGGCAAGCAGATGTGTGCGTTCACATACTTGAGGATGAACCCGCAACTATTTCAGGCCGACAACTGGAGGCGTTTTGTGTCATTTGTGAAGAAAATGAAGGAAGGAAAGAACGCTCAAAAGTGTAGGGAACAGGTGGAGAGGGAAGCCGAGCATTTCGTGCACGTTACAAGACCTTTGGTGCAGGAGGCTGCTGTTGCTCTGATGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016052 GO:0016160 GO:0016161 GO:0016787 GO:0016798 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_14

Protein Analysis

674

Amino Acids

74.56

Weight (kDa)

6.22

Isoelectric Point (pI)

49.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_14	PF01373	252 - 633	1.7e-152	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013127)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23920
fragaria_vesca	FvH4_4g05230
malus_domestica	MD12G1073900.v1.1 MD13G1226400.v1.1 MD16G1231500.v1.1
prunus_persica	Prupe.1G053800_v2.0.a1
pyrus_communis	pycom03g22190 pycom13g19950 pycom16g19370
rosa_chinensis	RchiOBHm_Chr4g0396151
rosa_laevigata	RLG00000009565
rosa_multiflora	Rmu_sc0006325.1_g000039
rosa_roxburghii	Rroxscaffold_5G00341000
rosa_rugosa	Rorug03G0351400
rosa_samantha	Rh4BG065100 Rh4CG071900 Rh4DG061900
rosa_wichuraiana	Rw4G005380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1124, 1718
Acc36I	ACCTGC	2 cut(s)	1124, 1718
AccB1I	GGYRCC	2 cut(s)	1196, 1601
AccII	CGCG	7 cut(s)	264, 266, 414, 564, 696, 773, 782
AclWI	GGATC	6 cut(s)	236, 544, 741, 932, 1417, 1430
AcoI	YGGCCR	6 cut(s)	47, 340, 531, 819, 1313, 1698
AcsI	RAATTY	2 cut(s)	451, 1373
AcuI	CTGAAG	4 cut(s)	687, 1051, 1318, 1833
AcvI	CACGTG	1 cut(s)	207
AcyI	GRCGYC	3 cut(s)	92, 310, 348
AdeI	CACNNNGTG	1 cut(s)	671
AfaI	GTAC	6 cut(s)	561, 785, 953, 1391, 1541, 1719
AfeI	AGCGCT	1 cut(s)	407
AflII	CTTAAG	1 cut(s)	164
AflIII	ACRYGT	2 cut(s)	71, 745
AgsI	TTSAA	4 cut(s)	713, 1081, 1612, 1795
AjiI	CACGTC	2 cut(s)	445, 746
AjnI	CCWGG	2 cut(s)	553, 1186
AjuI	GAANNNNNNNTTGG	2 cut(s)	1101, 1133
AleI	CACNNNNGTG	2 cut(s)	1034, 1103
AluBI	AGCT	6 cut(s)	100, 1141, 1233, 1241, 1528, 1678
AluI	AGCT	6 cut(s)	100, 1141, 1233, 1241, 1528, 1678
Alw21I	GWGCWC	5 cut(s)	338, 586, 1143, 1530, 1977
Alw26I	GTCTC	1 cut(s)	301
Alw44I	GTGCAC	1 cut(s)	1973
AlwI	GGATC	6 cut(s)	236, 544, 741, 932, 1417, 1430
Ama87I	CYCGRG	3 cut(s)	229, 519, 1523
Aor51HI	AGCGCT	1 cut(s)	407
ApaLI	GTGCAC	1 cut(s)	1973
ApeKI	GCWGC	3 cut(s)	100, 1645, 2003
ApoI	RAATTY	2 cut(s)	451, 1373
AspA2I	CCTAGG	1 cut(s)	1381
AspLEI	GCGC	6 cut(s)	266, 408, 720, 775, 782, 1001
AspS9I	GGNCC	9 cut(s)	17, 140, 221, 595, 1015, 1127, 1283, 1408, 1512
AsuC2I	CCSGG	9 cut(s)	230, 231, 344, 430, 530, 602, 777, 1510, 1559
AsuHPI	GGTGA	9 cut(s)	371, 418, 428, 683, 1106, 1148, 1382, 1427, 1742
AvaI	CYCGRG	3 cut(s)	229, 519, 1523
AvaII	GGWCC	5 cut(s)	140, 1015, 1127, 1408, 1512
AvrII	CCTAGG	1 cut(s)	1381
BaeGI	GKGCMC	1 cut(s)	1977
BalI	TGGCCA	2 cut(s)	821, 1700
BamHI	GGATCC	1 cut(s)	1422
BanI	GGYRCC	2 cut(s)	1196, 1601
BanII	GRGCYC	3 cut(s)	501, 1143, 1530
BauI	CACGAG	3 cut(s)	585, 1640, 1766
BbrPI	CACGTG	1 cut(s)	207
BbsI	GAAGAC	1 cut(s)	693
Bbv12I	GWGCWC	5 cut(s)	338, 586, 1143, 1530, 1977
BbvCI	CCTCAGC	1 cut(s)	1432
BbvI	GCAGC	3 cut(s)	87, 1657, 1990
BccI	CCATC	9 cut(s)	136, 232, 324, 734, 811, 1117, 1163, 1170, 1556
BceAI	ACGGC	8 cut(s)	62, 267, 409, 476, 626, 632, 833, 847
BciT130I	CCWGG	2 cut(s)	555, 1188
BclI	TGATCA	2 cut(s)	291, 958
BcnI	CCSGG	9 cut(s)	230, 231, 344, 430, 530, 602, 777, 1510, 1559
BcoDI	GTCTC	1 cut(s)	301
BfaI	CTAG	1 cut(s)	1382
BfoI	RGCGCY	2 cut(s)	409, 1002
BfrI	CTTAAG	1 cut(s)	164
BfuAI	ACCTGC	2 cut(s)	1124, 1718
BglII	AGATCT	1 cut(s)	1773
BisI	GCNGC	7 cut(s)	101, 211, 598, 871, 1027, 1646, 2004
BlnI	CCTAGG	1 cut(s)	1381
BlsI	GCNGC	7 cut(s)	102, 212, 599, 872, 1028, 1647, 2005
Bme18I	GGWCC	5 cut(s)	140, 1015, 1127, 1408, 1512
BmeT110I	CYCGRG	3 cut(s)	229, 519, 1523
BmgBI	CACGTC	2 cut(s)	445, 746
BmgT120I	GGNCC	9 cut(s)	17, 140, 221, 595, 1015, 1127, 1283, 1408, 1512
BmiI	GGNNCC	9 cut(s)	367, 571, 1128, 1198, 1285, 1424, 1507, 1514, 1603
BmrI	ACTGGG	2 cut(s)	903, 1390
BmsI	GCATC	7 cut(s)	469, 628, 1282, 1497, 1620, 1793, 2007
BmuI	ACTGGG	2 cut(s)	903, 1390
BpiI	GAAGAC	1 cut(s)	693
BpmI	CTGGAG	1 cut(s)	1900
Bpu10I	CCTNAGC	1 cut(s)	1432
BpuEI	CTTGAG	1 cut(s)	1864
BpuMI	CCSGG	9 cut(s)	230, 231, 344, 430, 530, 602, 777, 1510, 1559
BsaAI	YACGTR	2 cut(s)	72, 207
BsaHI	GRCGYC	3 cut(s)	92, 310, 348
BsaWI	WCCGGW	3 cut(s)	59, 572, 1012
Bse118I	RCCGGY	5 cut(s)	255, 505, 512, 623, 1295
Bse1I	ACTGG	7 cut(s)	190, 343, 909, 1034, 1316, 1396, 1883
Bse3DI	GCAATG	2 cut(s)	1046, 1739
BseBI	CCWGG	2 cut(s)	555, 1188
BseGI	GGATG	8 cut(s)	224, 294, 484, 1128, 1174, 1488, 1567, 1854
BseMI	GCAATG	2 cut(s)	1046, 1739
BseMII	CTCAG	8 cut(s)	119, 1217, 1245, 1446, 1485, 1595, 1662, 1718
BseNI	ACTGG	7 cut(s)	190, 343, 909, 1034, 1316, 1396, 1883
BseRI	GAGGAG	1 cut(s)	938
BseSI	GKGCMC	1 cut(s)	1977
BseX3I	CGGCCG	2 cut(s)	47, 531
BseXI	GCAGC	3 cut(s)	87, 1657, 1990
BsgI	GTGCAG	1 cut(s)	2015
Bsh1236I	CGCG	7 cut(s)	264, 266, 414, 564, 696, 773, 782
Bsh1285I	CGRYCG	3 cut(s)	50, 510, 534
BshNI	GGYRCC	2 cut(s)	1196, 1601
BsiEI	CGRYCG	3 cut(s)	50, 510, 534
BsiHKAI	GWGCWC	5 cut(s)	338, 586, 1143, 1530, 1977
BsiHKCI	CYCGRG	3 cut(s)	229, 519, 1523
BsiWI	CGTACG	1 cut(s)	559
BslFI	GGGAC	7 cut(s)	178, 246, 359, 446, 782, 899, 1498
BsmAI	GTCTC	1 cut(s)	301
BsmBI	CGTCTC	1 cut(s)	301
BsmFI	GGGAC	7 cut(s)	178, 246, 359, 446, 782, 899, 1498
BsmI	GAATGC	1 cut(s)	15
BsoBI	CYCGRG	3 cut(s)	229, 519, 1523
Bsp1286I	GDGCHC	6 cut(s)	338, 501, 586, 1143, 1530, 1977
Bsp143I	GATC	9 cut(s)	241, 291, 328, 536, 733, 937, 958, 1422, 1773
Bsp19I	CCATGG	2 cut(s)	1411, 1597
BspCNI	CTCAG	8 cut(s)	118, 1218, 1246, 1445, 1484, 1594, 1663, 1717
BspFNI	CGCG	7 cut(s)	264, 266, 414, 564, 696, 773, 782
BspHI	TCATGA	1 cut(s)	736
BspLI	GGNNCC	9 cut(s)	367, 571, 1128, 1198, 1285, 1424, 1507, 1514, 1603
BspMI	ACCTGC	2 cut(s)	1124, 1718
BspPI	GGATC	6 cut(s)	236, 544, 741, 932, 1417, 1430
BspQI	GCTCTTC	1 cut(s)	708
BspT107I	GGYRCC	2 cut(s)	1196, 1601
BspTI	CTTAAG	1 cut(s)	164
BsrDI	GCAATG	2 cut(s)	1046, 1739
BsrFI	RCCGGY	5 cut(s)	255, 505, 512, 623, 1295
BsrI	ACTGG	7 cut(s)	190, 343, 909, 1034, 1316, 1396, 1883
BssAI	RCCGGY	5 cut(s)	255, 505, 512, 623, 1295
BssMI	GATC	9 cut(s)	241, 291, 328, 536, 733, 937, 958, 1422, 1773
BssNI	GRCGYC	3 cut(s)	92, 310, 348
BssSI	CACGAG	3 cut(s)	585, 1640, 1766
BssT1I	CCWWGG	5 cut(s)	1381, 1411, 1597, 1695, 1745
Bst2BI	CACGAG	3 cut(s)	585, 1640, 1766
Bst2UI	CCWGG	2 cut(s)	555, 1188
Bst4CI	ACNGT	3 cut(s)	250, 671, 1370
Bst6I	CTCTTC	2 cut(s)	708, 1333
BstACI	GRCGYC	3 cut(s)	92, 310, 348
BstAFI	CTTAAG	1 cut(s)	164
BstAPI	GCANNNNNTGC	2 cut(s)	1777, 1972
BstBAI	YACGTR	2 cut(s)	72, 207
BstC8I	GCNNGC	9 cut(s)	398, 551, 621, 1031, 1062, 1486, 1594, 1782, 1820
BstDEI	CTNAG	8 cut(s)	105, 1226, 1254, 1432, 1471, 1581, 1671, 1704
BstDSI	CCRYGG	4 cut(s)	44, 412, 1411, 1597
BstEII	GGTNACC	1 cut(s)	1565
BstF5I	GGATG	8 cut(s)	224, 294, 484, 1128, 1174, 1488, 1567, 1854
BstFNI	CGCG	7 cut(s)	264, 266, 414, 564, 696, 773, 782
BstH2I	RGCGCY	2 cut(s)	409, 1002
BstHHI	GCGC	6 cut(s)	266, 408, 720, 775, 782, 1001
BstKTI	GATC	9 cut(s)	244, 294, 331, 539, 736, 940, 961, 1425, 1776
BstMAI	GTCTC	1 cut(s)	301
BstMBI	GATC	9 cut(s)	241, 291, 328, 536, 733, 937, 958, 1422, 1773
BstMCI	CGRYCG	3 cut(s)	50, 510, 534
BstNI	CCWGG	2 cut(s)	555, 1188
BstNSI	RCATGY	1 cut(s)	162
BstPI	GGTNACC	1 cut(s)	1565
BstSLI	GKGCMC	1 cut(s)	1977
BstUI	CGCG	7 cut(s)	264, 266, 414, 564, 696, 773, 782
BstV1I	GCAGC	3 cut(s)	87, 1657, 1990
BstV2I	GAAGAC	1 cut(s)	693
BstX2I	RGATCY	4 cut(s)	241, 536, 1422, 1773
BstYI	RGATCY	4 cut(s)	241, 536, 1422, 1773
BstZI	CGGCCG	2 cut(s)	47, 531
BtgI	CCRYGG	4 cut(s)	44, 412, 1411, 1597
BtgZI	GCGATG	1 cut(s)	702
BtrI	CACGTC	2 cut(s)	445, 746
BtsCI	GGATG	8 cut(s)	224, 294, 484, 1128, 1174, 1488, 1567, 1854
BtsIMutI	CAGTG	6 cut(s)	336, 954, 1041, 1287, 1809, 2020
BveI	ACCTGC	2 cut(s)	1124, 1718
Cac8I	GCNNGC	9 cut(s)	398, 551, 621, 1031, 1062, 1486, 1594, 1782, 1820
CciI	TCATGA	1 cut(s)	736
CfoI	GCGC	6 cut(s)	266, 408, 720, 775, 782, 1001
Cfr10I	RCCGGY	5 cut(s)	255, 505, 512, 623, 1295
Cfr13I	GGNCC	9 cut(s)	17, 140, 221, 595, 1015, 1127, 1283, 1408, 1512
Cfr42I	CCGCGG	1 cut(s)	415
Cfr9I	CCCGGG	1 cut(s)	229
CseI	GACGC	5 cut(s)	100, 255, 318, 356, 779
Csp6I	GTAC	6 cut(s)	560, 784, 952, 1390, 1540, 1718
CviQI	GTAC	6 cut(s)	560, 784, 952, 1390, 1540, 1718
DdeI	CTNAG	8 cut(s)	105, 1226, 1254, 1432, 1471, 1581, 1671, 1704
DpnI	GATC	9 cut(s)	243, 293, 330, 538, 735, 939, 960, 1424, 1775
DpnII	GATC	9 cut(s)	241, 291, 328, 536, 733, 937, 958, 1422, 1773
DraIII	CACNNNGTG	1 cut(s)	671
EaeI	YGGCCR	6 cut(s)	47, 340, 531, 819, 1313, 1698
EagI	CGGCCG	2 cut(s)	47, 531
Eam1104I	CTCTTC	2 cut(s)	708, 1333
EarI	CTCTTC	2 cut(s)	708, 1333
EciI	GGCGGA	1 cut(s)	242
Ecl136II	GAGCTC	2 cut(s)	1141, 1528
EclXI	CGGCCG	2 cut(s)	47, 531
Eco130I	CCWWGG	5 cut(s)	1381, 1411, 1597, 1695, 1745
Eco24I	GRGCYC	3 cut(s)	501, 1143, 1530
Eco32I	GATATC	1 cut(s)	355
Eco47I	GGWCC	5 cut(s)	140, 1015, 1127, 1408, 1512
Eco47III	AGCGCT	1 cut(s)	407
Eco52I	CGGCCG	2 cut(s)	47, 531
Eco53kI	GAGCTC	2 cut(s)	1141, 1528
Eco57I	CTGAAG	4 cut(s)	687, 1051, 1318, 1833
Eco72I	CACGTG	1 cut(s)	207
Eco88I	CYCGRG	3 cut(s)	229, 519, 1523
Eco91I	GGTNACC	1 cut(s)	1565
EcoICRI	GAGCTC	2 cut(s)	1141, 1528
EcoO109I	RGGNCCY	1 cut(s)	1283
EcoO65I	GGTNACC	1 cut(s)	1565
EcoRI	GAATTC	1 cut(s)	1373
EcoRII	CCWGG	2 cut(s)	553, 1186
EcoRV	GATATC	1 cut(s)	355
EcoT14I	CCWWGG	5 cut(s)	1381, 1411, 1597, 1695, 1745
EcoT22I	ATGCAT	2 cut(s)	15, 1741
EcoT38I	GRGCYC	3 cut(s)	501, 1143, 1530
ErhI	CCWWGG	5 cut(s)	1381, 1411, 1597, 1695, 1745
Esp3I	CGTCTC	1 cut(s)	301
FaqI	GGGAC	7 cut(s)	178, 246, 359, 446, 782, 899, 1498
FauI	CCCGC	4 cut(s)	208, 496, 1601, 1863
FbaI	TGATCA	2 cut(s)	291, 958
Fnu4HI	GCNGC	7 cut(s)	101, 211, 598, 871, 1027, 1646, 2004
FokI	GGATG	8 cut(s)	211, 281, 491, 1135, 1181, 1475, 1574, 1861
FriOI	GRGCYC	3 cut(s)	501, 1143, 1530
Fsp4HI	GCNGC	7 cut(s)	101, 211, 598, 871, 1027, 1646, 2004
FspBI	CTAG	1 cut(s)	1382
GlaI	GCGC	6 cut(s)	265, 407, 719, 774, 781, 1000
GluI	GCNGC	7 cut(s)	101, 211, 598, 871, 1027, 1646, 2004
GsuI	CTGGAG	1 cut(s)	1900
HaeII	RGCGCY	2 cut(s)	409, 1002
HgaI	GACGC	5 cut(s)	100, 255, 318, 356, 779
HhaI	GCGC	6 cut(s)	266, 408, 720, 775, 782, 1001
Hin1I	GRCGYC	3 cut(s)	92, 310, 348
Hin6I	GCGC	6 cut(s)	264, 406, 718, 773, 780, 999
HinP1I	GCGC	6 cut(s)	264, 406, 718, 773, 780, 999
HincII	GTYRAC	1 cut(s)	931
HindII	GTYRAC	1 cut(s)	931
HindIII	AAGCTT	2 cut(s)	1239, 1676
HinfI	GANTC	6 cut(s)	387, 674, 698, 887, 1250, 1554
HpaI	GTTAAC	1 cut(s)	931
HphI	GGTGA	9 cut(s)	371, 418, 428, 683, 1106, 1148, 1382, 1427, 1742
Hpy166II	GTNNAC	6 cut(s)	90, 614, 931, 954, 1836, 1975
Hpy188III	TCNNGA	4 cut(s)	239, 737, 941, 1160
Hpy8I	GTNNAC	6 cut(s)	90, 614, 931, 954, 1836, 1975
Hpy99I	CGWCG	4 cut(s)	271, 288, 395, 884
HpyCH4III	ACNGT	3 cut(s)	250, 671, 1370
HpyCH4IV	ACGT	6 cut(s)	71, 206, 444, 745, 879, 1977
HpyF3I	CTNAG	8 cut(s)	105, 1226, 1254, 1432, 1471, 1581, 1671, 1704
HpySE526I	ACGT	6 cut(s)	71, 206, 444, 745, 879, 1977
Hsp92I	GRCGYC	3 cut(s)	92, 310, 348
HspAI	GCGC	6 cut(s)	264, 406, 718, 773, 780, 999
Ksp22I	TGATCA	2 cut(s)	291, 958
KspAI	GTTAAC	1 cut(s)	931
KspI	CCGCGG	1 cut(s)	415
Kzo9I	GATC	9 cut(s)	241, 291, 328, 536, 733, 937, 958, 1422, 1773
LguI	GCTCTTC	1 cut(s)	708
LmnI	GCTCC	5 cut(s)	333, 365, 575, 581, 1146
Lsp1109I	GCAGC	3 cut(s)	87, 1657, 1990
LweI	GCATC	7 cut(s)	469, 628, 1282, 1497, 1620, 1793, 2007
MaeI	CTAG	1 cut(s)	1382
MaeII	ACGT	6 cut(s)	71, 206, 444, 745, 879, 1977
MalI	GATC	9 cut(s)	243, 293, 330, 538, 735, 939, 960, 1424, 1775
MboI	GATC	9 cut(s)	241, 291, 328, 536, 733, 937, 958, 1422, 1773
MboII	GAAGA	8 cut(s)	692, 693, 725, 1324, 1331, 1350, 1369, 1915
MfeI	CAATTG	1 cut(s)	787
MflI	RGATCY	4 cut(s)	241, 536, 1422, 1773
MhlI	GDGCHC	6 cut(s)	338, 501, 586, 1143, 1530, 1977
MlsI	TGGCCA	2 cut(s)	821, 1700
MluCI	AATT	5 cut(s)	451, 787, 1191, 1373, 1612
MluNI	TGGCCA	2 cut(s)	821, 1700
MlyI	GAGTC	2 cut(s)	707, 881
MmeI	TCCRAC	1 cut(s)	625
Mox20I	TGGCCA	2 cut(s)	821, 1700
Mph1103I	ATGCAT	2 cut(s)	15, 1741
MscI	TGGCCA	2 cut(s)	821, 1700
MseI	TTAA	4 cut(s)	165, 930, 1020, 1235
MslI	CAYNNNNRTG	4 cut(s)	299, 1034, 1103, 1628
Msp20I	TGGCCA	2 cut(s)	821, 1700
MspA1I	CMGCKG	1 cut(s)	414
MspCI	CTTAAG	1 cut(s)	164
MunI	CAATTG	1 cut(s)	787
Mva1269I	GAATGC	1 cut(s)	15
MvaI	CCWGG	2 cut(s)	555, 1188
MvnI	CGCG	7 cut(s)	264, 266, 414, 564, 696, 773, 782
NciI	CCSGG	9 cut(s)	230, 231, 344, 430, 530, 602, 777, 1510, 1559
NcoI	CCATGG	2 cut(s)	1411, 1597
NdeII	GATC	9 cut(s)	241, 291, 328, 536, 733, 937, 958, 1422, 1773
NlaIV	GGNNCC	9 cut(s)	367, 571, 1128, 1198, 1285, 1424, 1507, 1514, 1603
NmeAIII	GCCGAG	1 cut(s)	1987
NmuCI	GTSAC	6 cut(s)	258, 359, 424, 653, 1094, 1298
NsiI	ATGCAT	2 cut(s)	15, 1741
NspI	RCATGY	1 cut(s)	162
OliI	CACNNNNGTG	2 cut(s)	1034, 1103
PagI	TCATGA	1 cut(s)	736
PaqCI	CACCTGC	2 cut(s)	1124, 1718
PciSI	GCTCTTC	1 cut(s)	708
PcsI	WCGNNNNNNNCGW	2 cut(s)	92, 441
PctI	GAATGC	1 cut(s)	15
PfeI	GAWTC	4 cut(s)	387, 674, 1250, 1554
Pfl23II	CGTACG	1 cut(s)	559
PfoI	TCCNGGA	2 cut(s)	1186, 1557
PkrI	GCNGC	7 cut(s)	102, 212, 599, 872, 1028, 1647, 2005
PleI	GAGTC	2 cut(s)	706, 881
PmaCI	CACGTG	1 cut(s)	207
PmlI	CACGTG	1 cut(s)	207
PpsI	GAGTC	2 cut(s)	706, 881
Ppu21I	YACGTR	2 cut(s)	72, 207
Psp124BI	GAGCTC	2 cut(s)	1143, 1530
Psp6I	CCWGG	2 cut(s)	553, 1186
PspCI	CACGTG	1 cut(s)	207
PspEI	GGTNACC	1 cut(s)	1565
PspGI	CCWGG	2 cut(s)	553, 1186
PspLI	CGTACG	1 cut(s)	559
PspN4I	GGNNCC	9 cut(s)	367, 571, 1128, 1198, 1285, 1424, 1507, 1514, 1603
PspPI	GGNCC	9 cut(s)	17, 140, 221, 595, 1015, 1127, 1283, 1408, 1512
PsuI	RGATCY	4 cut(s)	241, 536, 1422, 1773
RsaI	GTAC	6 cut(s)	561, 785, 953, 1391, 1541, 1719
RsaNI	GTAC	6 cut(s)	560, 784, 952, 1390, 1540, 1718
RseI	CAYNNNNRTG	4 cut(s)	299, 1034, 1103, 1628
SacI	GAGCTC	2 cut(s)	1143, 1530
SacII	CCGCGG	1 cut(s)	415
SapI	GCTCTTC	1 cut(s)	708
SaqAI	TTAA	4 cut(s)	165, 930, 1020, 1235
SatI	GCNGC	7 cut(s)	101, 211, 598, 871, 1027, 1646, 2004
Sau3AI	GATC	9 cut(s)	241, 291, 328, 536, 733, 937, 958, 1422, 1773
Sau96I	GGNCC	9 cut(s)	17, 140, 221, 595, 1015, 1127, 1283, 1408, 1512
SchI	GAGTC	2 cut(s)	707, 881
SduI	GDGCHC	6 cut(s)	338, 501, 586, 1143, 1530, 1977
SfaNI	GCATC	7 cut(s)	469, 628, 1282, 1497, 1620, 1793, 2007
Sfr303I	CCGCGG	1 cut(s)	415
SgrBI	CCGCGG	1 cut(s)	415
SinI	GGWCC	5 cut(s)	140, 1015, 1127, 1408, 1512
SmaI	CCCGGG	1 cut(s)	231
SmiMI	CAYNNNNRTG	4 cut(s)	299, 1034, 1103, 1628
SmlI	CTYRAG	2 cut(s)	164, 1843
SmoI	CTYRAG	2 cut(s)	164, 1843
Sse9I	AATT	5 cut(s)	451, 787, 1191, 1373, 1612
SspMI	CTAG	1 cut(s)	1382
SstI	GAGCTC	2 cut(s)	1143, 1530
StyI	CCWWGG	5 cut(s)	1381, 1411, 1597, 1695, 1745
TaaI	ACNGT	3 cut(s)	250, 671, 1370
TaiI	ACGT	6 cut(s)	74, 209, 447, 748, 882, 1980
TaqI	TCGA	2 cut(s)	269, 390
TasI	AATT	5 cut(s)	451, 787, 1191, 1373, 1612
TauI	GCSGC	4 cut(s)	213, 600, 873, 1029
TfiI	GAWTC	4 cut(s)	387, 674, 1250, 1554
Tru1I	TTAA	4 cut(s)	165, 930, 1020, 1235
Tru9I	TTAA	4 cut(s)	165, 930, 1020, 1235
TscAI	CASTG	5 cut(s)	343, 961, 1041, 1294, 1816
TseFI	GTSAC	6 cut(s)	258, 359, 424, 653, 1094, 1298
TseI	GCWGC	3 cut(s)	100, 1645, 2003
Tsp45I	GTSAC	6 cut(s)	258, 359, 424, 653, 1094, 1298
TspDTI	ATGAA	6 cut(s)	675, 705, 1045, 1143, 1865, 1925
TspGWI	ACGGA	2 cut(s)	378, 1133
TspMI	CCCGGG	1 cut(s)	229
TspRI	CASTG	5 cut(s)	343, 961, 1041, 1294, 1816
Vha464I	CTTAAG	1 cut(s)	164
VneI	GTGCAC	1 cut(s)	1973
VpaK11BI	GGWCC	5 cut(s)	140, 1015, 1127, 1408, 1512
XapI	RAATTY	2 cut(s)	451, 1373
XceI	RCATGY	1 cut(s)	162
XcmI	CCANNNNNNNNNTGG	2 cut(s)	916, 1499
XmaI	CCCGGG	1 cut(s)	229
XmaJI	CCTAGG	1 cut(s)	1381
XspI	CTAG	1 cut(s)	1382
Zsp2I	ATGCAT	2 cut(s)	15, 1741

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.