MD13G1246700.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014920
Physical Location & Seq
Forward (+)
25989270 .. 25991264
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1246700.v1.1.491

Sequence Viewer

Length: 981 bp
ATGGAAACTCGTAGTTGCATTCTCCTATCTGCTTTTCTCATCCCTTTCCTTCTTGGAACAGCTTGTGCTCAACTTAGGACAGACTTCTACAAAGGCACGTGTCCAAATCTTGAGTCCATAGTCTCTTCTGCTGTCAAAACCAAGTTCCAGCAGACTTTTGTAACTGCTCCAGCCACTTTGAGGCTCTTCTTCCACGACTGCTTTGTTCGGGGTTGTGATGCTTCAGTGTTGATTCAGTCACCAACTAATCAGGGGGAGAAGGATCACCCCGATAACCTTTCGCTTGCTGGAGATGGATTTGACACTGTGATCAAAGCCAAGGCAGCGGTTGATGGCGATCCTAACTGCAAAAACAAGGTTTCATGTGCAGATATTCTTGCTCTGGCTACTAGAGATGTTGTACAGCTGGCTGGGGGACCATCTTATACAGTTGAACTGGGTAGGCGAGATGGAAGGGTATCTACTATGGCTAGTGTTCAGAGACGCCTTCCTTCCCCTACTTTCAATCTAGACCAGCTTAACACCATGTTTACCTCTCATGGTCTCTCCCAGACTGACATGGTTGCATTATCAGGTGCGCACACACTTGGTTTCTCTCATTGCAGCCGGTTTTCCAAGAGAATCTACAGCTTCAGCAGCAAAACCCGTATTGATCCGACACTCGATCCAGCTTATGCATTGCAGCTTCGACAGATGTGTCCAACTCGAGTGGATCCCAGAATTGCCATTAACATGGACCCAACCACACCTCGAGCATTTGACAATGTTTACTTCCAGAATCTTCAGCAAGGAAAGGGTCTGTTCACCTCTGATCAGATACTGTTTACAGATACAAGAACAAGGGCAACCGTCAACCAATTTGCATCAAGTAATGCAGCTTTTAATCAAGCTTTTATACAAGCCATGACTAAGTTAGGCAGAATTGGGGTTCTGACGGGCAATCAAGGCGAGATTCGGCGAGACTGCACTCGCCCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

327

Amino Acids

35.77

Weight (kDa)

9.05

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 289 1.6e-83 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014920)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18980 AT4G30170
fragaria_vesca FvH4_4g07750
malus_domestica MD13G1246700.v1.1
prunus_persica Prupe.1G081600_v2.0.a1
pyrus_communis pycom13g21780
rosa_chinensis RchiOBHm_Chr4g0400821
rosa_laevigata RLG00000009178
rosa_multiflora Rmu_sc0004591.1_g000015
rosa_roxburghii Rroxscaffold_5G00345640
rosa_rugosa Rorug04G0026100
rosa_samantha Rh4AG104000 Rh4BG099000 Rh4CG112200 Rh4DG095800
rosa_wichuraiana Rw4G008410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 696
Acc16I TGCGCA 1 cut(s) 579
AciI CCGC 1 cut(s) 326
AclWI GGATC 6 cut(s) 270, 332, 647, 659, 707, 720
AcuI CTGAAG 3 cut(s) 207, 616, 767
AcvI CACGTG 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 484
AfaI GTAC 1 cut(s) 402
AfiI CCNNNNNNNGG 1 cut(s) 180
AflIII ACRYGT 1 cut(s) 98
AgsI TTSAA 2 cut(s) 434, 505
AluBI AGCT 8 cut(s) 62, 406, 517, 630, 671, 685, 878, 890
AluI AGCT 8 cut(s) 62, 406, 517, 630, 671, 685, 878, 890
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 4 cut(s) 127, 475, 548, 954
AlwI GGATC 6 cut(s) 270, 332, 647, 659, 707, 720
AlwNI CAGNNNCTG 1 cut(s) 820
Ama87I CYCGRG 2 cut(s) 705, 750
ApeKI GCWGC 5 cut(s) 323, 603, 636, 682, 875
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
AspLEI GCGC 1 cut(s) 580
AspS9I GGNCC 2 cut(s) 416, 736
AsuHPI GGTGA 3 cut(s) 231, 257, 796
AvaI CYCGRG 2 cut(s) 705, 750
AvaII GGWCC 2 cut(s) 416, 736
BamHI GGATCC 1 cut(s) 712
BarI GAAGNNNNNNTAC 2 cut(s) 445, 477
BbrPI CACGTG 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 5 cut(s) 335, 615, 648, 694, 887
BccI CCATC 4 cut(s) 287, 326, 427, 443
BclI TGATCA 2 cut(s) 309, 811
BcoDI GTCTC 4 cut(s) 127, 475, 548, 954
BfaI CTAG 4 cut(s) 390, 471, 509, 979
BfmI CTRYAG 1 cut(s) 625
BisI GCNGC 5 cut(s) 324, 604, 637, 683, 876
BlsI GCNGC 5 cut(s) 325, 605, 638, 684, 877
Bme18I GGWCC 2 cut(s) 416, 736
BmeT110I CYCGRG 2 cut(s) 705, 750
BmgT120I GGNCC 2 cut(s) 416, 736
BmiI GGNNCC 3 cut(s) 417, 714, 738
BmrI ACTGGG 1 cut(s) 446
BmsI GCATC 2 cut(s) 208, 872
BmuI ACTGGG 1 cut(s) 446
BpmI CTGGAG 2 cut(s) 153, 309
BpuEI CTTGAG 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 484
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 1 cut(s) 318
Bsc4I CCNNNNNNNGG 1 cut(s) 180
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 1 cut(s) 441
Bse3DI GCAATG 2 cut(s) 598, 677
Bse8I GATNNNNATC 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 1 cut(s) 39
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 1 cut(s) 180
BseMI GCAATG 2 cut(s) 598, 677
BseNI ACTGG 1 cut(s) 441
BseXI GCAGC 5 cut(s) 335, 615, 648, 694, 887
BseYI CCCAGC 1 cut(s) 410
BsgI GTGCAG 2 cut(s) 387, 949
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 2 cut(s) 705, 750
BsiSI CCGG 1 cut(s) 607
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 1 cut(s) 180
BsmAI GTCTC 4 cut(s) 127, 475, 548, 954
BsmBI CGTCTC 1 cut(s) 475
BsmFI GGGAC 1 cut(s) 429
BsmI GAATGC 1 cut(s) 18
Bso31I GGTCTC 1 cut(s) 548
BsoBI CYCGRG 2 cut(s) 705, 750
Bsp1286I GDGCHC 1 cut(s) 70
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 7 cut(s) 262, 309, 337, 652, 664, 712, 811
BspACI CCGC 1 cut(s) 326
BspLI GGNNCC 3 cut(s) 417, 714, 738
BspPI GGATC 6 cut(s) 270, 332, 647, 659, 707, 720
BspQI GCTCTTC 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 2 cut(s) 598, 677
BsrFI RCCGGY 1 cut(s) 606
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 1 cut(s) 441
BssAI RCCGGY 1 cut(s) 606
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 7 cut(s) 262, 309, 337, 652, 664, 712, 811
BssNI GRCGYC 1 cut(s) 484
BssT1I CCWWGG 1 cut(s) 318
Bst4CI ACNGT 4 cut(s) 307, 430, 822, 850
Bst6I CTCTTC 2 cut(s) 130, 191
BstACI GRCGYC 1 cut(s) 484
BstAUI TGTACA 1 cut(s) 400
BstBAI YACGTR 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 285, 408
BstDEI CTNAG 2 cut(s) 74, 909
BstF5I GGATG 1 cut(s) 39
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 7 cut(s) 265, 312, 340, 655, 667, 715, 814
BstMAI GTCTC 4 cut(s) 127, 475, 548, 954
BstMBI GATC 7 cut(s) 262, 309, 337, 652, 664, 712, 811
BstMWI GCNNNNNNNGC 3 cut(s) 323, 636, 945
BstSFI CTRYAG 1 cut(s) 625
BstV1I GCAGC 5 cut(s) 335, 615, 648, 694, 887
BstX2I RGATCY 1 cut(s) 712
BstXI CCANNNNNNTGG 1 cut(s) 733
BstYI RGATCY 1 cut(s) 712
BtsCI GGATG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 231, 303
Cac8I GCNNGC 2 cut(s) 285, 408
CaiI CAGNNNCTG 1 cut(s) 820
CfoI GCGC 1 cut(s) 580
Cfr10I RCCGGY 1 cut(s) 606
Cfr13I GGNCC 2 cut(s) 416, 736
CseI GACGC 1 cut(s) 492
Csp6I GTAC 1 cut(s) 401
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviAII CATG 6 cut(s) 363, 526, 539, 559, 733, 904
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 2 cut(s) 74, 909
DpnI GATC 7 cut(s) 264, 311, 339, 654, 666, 714, 813
DpnII GATC 7 cut(s) 262, 309, 337, 652, 664, 712, 811
DrdI GACNNNNNNGTC 1 cut(s) 696
DseDI GACNNNNNNGTC 1 cut(s) 696
Eam1104I CTCTTC 2 cut(s) 130, 191
EarI CTCTTC 2 cut(s) 130, 191
Eco130I CCWWGG 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 2 cut(s) 416, 736
Eco57I CTGAAG 3 cut(s) 207, 616, 767
Eco72I CACGTG 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 705, 750
EcoT14I CCWWGG 1 cut(s) 318
EcoT22I ATGCAT 1 cut(s) 679
ErhI CCWWGG 1 cut(s) 318
Esp3I CGTCTC 1 cut(s) 475
FaeI CATG 6 cut(s) 366, 529, 542, 562, 736, 907
FaqI GGGAC 1 cut(s) 429
FatI CATG 6 cut(s) 362, 525, 538, 558, 732, 903
FbaI TGATCA 2 cut(s) 309, 811
Fnu4HI GCNGC 5 cut(s) 324, 604, 637, 683, 876
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 5 cut(s) 324, 604, 637, 683, 876
FspAI RTGCGCAY 1 cut(s) 579
FspBI CTAG 4 cut(s) 390, 471, 509, 979
FspI TGCGCA 1 cut(s) 579
GlaI GCGC 1 cut(s) 579
GluI GCNGC 5 cut(s) 324, 604, 637, 683, 876
GsaI CCCAGC 1 cut(s) 414
GsuI CTGGAG 2 cut(s) 153, 309
HapII CCGG 1 cut(s) 607
HgaI GACGC 1 cut(s) 492
HhaI GCGC 1 cut(s) 580
Hin1I GRCGYC 1 cut(s) 484
Hin1II CATG 6 cut(s) 366, 529, 542, 562, 736, 907
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HindIII AAGCTT 1 cut(s) 888
HinfI GANTC 5 cut(s) 113, 232, 621, 778, 952
HpaII CCGG 1 cut(s) 607
HphI GGTGA 3 cut(s) 231, 257, 796
Hpy166II GTNNAC 5 cut(s) 531, 769, 804, 825, 853
Hpy188I TCNGA 5 cut(s) 480, 657, 811, 816, 933
Hpy188III TCNNGA 3 cut(s) 110, 509, 775
Hpy8I GTNNAC 5 cut(s) 531, 769, 804, 825, 853
HpyAV CCTTC 5 cut(s) 59, 253, 447, 497, 501
HpyCH4III ACNGT 4 cut(s) 307, 430, 822, 850
HpyCH4IV ACGT 1 cut(s) 98
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 636, 945
HpyF3I CTNAG 2 cut(s) 74, 909
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 1 cut(s) 484
Hsp92II CATG 6 cut(s) 366, 529, 542, 562, 736, 907
HspAI GCGC 1 cut(s) 578
Ksp22I TGATCA 2 cut(s) 309, 811
Kzo9I GATC 7 cut(s) 262, 309, 337, 652, 664, 712, 811
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 1 cut(s) 172
Lsp1109I GCAGC 5 cut(s) 335, 615, 648, 694, 887
LweI GCATC 2 cut(s) 208, 872
MaeI CTAG 4 cut(s) 390, 471, 509, 979
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 160, 237
MalI GATC 7 cut(s) 264, 311, 339, 654, 666, 714, 813
MboI GATC 7 cut(s) 262, 309, 337, 652, 664, 712, 811
MboII GAAGA 4 cut(s) 117, 178, 181, 773
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 3 cut(s) 720, 857, 921
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 2 cut(s) 680, 725
MnlI CCTC 4 cut(s) 174, 544, 759, 817
Mph1103I ATGCAT 1 cut(s) 679
MseI TTAA 3 cut(s) 519, 729, 882
MslI CAYNNNNRTG 1 cut(s) 731
MspA1I CMGCKG 2 cut(s) 326, 406
MspI CCGG 1 cut(s) 607
Mva1269I GAATGC 1 cut(s) 18
MwoI GCNNNNNNNGC 3 cut(s) 323, 636, 945
NdeII GATC 7 cut(s) 262, 309, 337, 652, 664, 712, 811
NlaIII CATG 6 cut(s) 366, 529, 542, 562, 736, 907
NlaIV GGNNCC 3 cut(s) 417, 714, 738
NmuCI GTSAC 1 cut(s) 237
NsbI TGCGCA 1 cut(s) 579
NsiI ATGCAT 1 cut(s) 679
PaeR7I CTCGAG 2 cut(s) 705, 750
PciSI GCTCTTC 1 cut(s) 191
PctI GAATGC 1 cut(s) 18
PfeI GAWTC 4 cut(s) 232, 621, 778, 952
PkrI GCNGC 5 cut(s) 325, 605, 638, 684, 877
PleI GAGTC 1 cut(s) 121
PmaCI CACGTG 1 cut(s) 99
PmlI CACGTG 1 cut(s) 99
PpsI GAGTC 1 cut(s) 121
Ppu21I YACGTR 1 cut(s) 99
PspCI CACGTG 1 cut(s) 99
PspFI CCCAGC 1 cut(s) 410
PspN4I GGNNCC 3 cut(s) 417, 714, 738
PspPI GGNCC 2 cut(s) 416, 736
PspXI VCTCGAGB 2 cut(s) 705, 750
PstNI CAGNNNCTG 1 cut(s) 820
PsuI RGATCY 1 cut(s) 712
PvuII CAGCTG 1 cut(s) 406
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
RseI CAYNNNNRTG 1 cut(s) 731
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 3 cut(s) 519, 729, 882
SatI GCNGC 5 cut(s) 324, 604, 637, 683, 876
Sau3AI GATC 7 cut(s) 262, 309, 337, 652, 664, 712, 811
Sau96I GGNCC 2 cut(s) 416, 736
SchI GAGTC 1 cut(s) 122
SduI GDGCHC 1 cut(s) 70
SfaNI GCATC 2 cut(s) 208, 872
SfcI CTRYAG 1 cut(s) 625
Sfr274I CTCGAG 2 cut(s) 705, 750
SinI GGWCC 2 cut(s) 416, 736
SlaI CTCGAG 2 cut(s) 705, 750
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 3 cut(s) 110, 705, 750
SmoI CTYRAG 3 cut(s) 110, 705, 750
Sse9I AATT 3 cut(s) 720, 857, 921
SsiI CCGC 1 cut(s) 326
SspMI CTAG 4 cut(s) 390, 471, 509, 979
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 4 cut(s) 307, 430, 822, 850
TaiI ACGT 1 cut(s) 101
TaqI TCGA 4 cut(s) 663, 688, 706, 751
TasI AATT 3 cut(s) 720, 857, 921
TatI WGTACW 1 cut(s) 400
TfiI GAWTC 4 cut(s) 232, 621, 778, 952
Tru1I TTAA 3 cut(s) 519, 729, 882
Tru9I TTAA 3 cut(s) 519, 729, 882
TscAI CASTG 2 cut(s) 231, 310
TseFI GTSAC 1 cut(s) 237
TseI GCWGC 5 cut(s) 323, 603, 636, 682, 875
Tsp45I GTSAC 1 cut(s) 237
TspDTI ATGAA 1 cut(s) 351
TspRI CASTG 2 cut(s) 231, 310
VpaK11BI GGWCC 2 cut(s) 416, 736
XbaI TCTAGA 1 cut(s) 508
XhoI CTCGAG 2 cut(s) 705, 750
XspI CTAG 4 cut(s) 390, 471, 509, 979
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.