MD13G1247100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016000
Physical Location & Seq
Reverse (-)
26051629 .. 26053607
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1247100.v1.1.491

Sequence Viewer

Length: 555 bp
ATGTCGTCCTCCATATCCTCATCTCAACAGAGCTTTCTAGCCAACGTTCTCACCCCCCCACCCCACAAACCCAAAACCAAGATTCTCATTTCTAACACTTTCATCTCATGCAAAATATTTGATGAAACGCCCAGAAAGGATGAGAACAAATTGGCAAAGTTGGCAATGGTGACACTGGCAGCTGGGGTGTTGACATTGGGCTCAGTTCATGATGCATCAGCTGCCAAGTCTGGTGGGAGAGTTGGAGGCCAGGCGTTTCGGTCTTCGACTCCTCGGTCTTCGTCCCCTAGGATCAACAACAATTCAAGGACCAATATCTACATCAATCCTCCAGTAGCACCACCTCTAGTTGGTGGCTATGGATATGGCTATGGAGTACCGTTCTTCGGAGGCTGGGGTTGGTCTCCATTTTCATTTTTTGCCCCAGGCCCTGGTGTTGCCATTGGCATCGGTGGCGGGTTCCAACTTTTCGCTTTCTTTGTGTTTCTTGGTGCTCTTGCTGCTGTTGCAAGGAGATTCACTGGATCAATAGACGAAGAAGAAGATGATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

185

Amino Acids

19.44

Weight (kDa)

9.52

Isoelectric Point (pI)

56.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57345
fragaria_vesca FvH4_4g07780
malus_domestica MD13G1247100.v1.1
pyrus_communis pycom13g21800
rosa_chinensis RchiOBHm_Chr4g0400871
rosa_laevigata RLG00000009171
rosa_multiflora Rmu_sc0004401.1_g000016 Rmu_sc0018346.1_g000005
rosa_roxburghii Rroxscaffold_5G00345750
rosa_rugosa Rorug04G0026500
rosa_samantha Rh4AG104500 Rh4BG099700 Rh4CG112900 Rh4DG096600
rosa_wichuraiana Rw4G008460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 274
AccB7I CCANNNNNTGG 1 cut(s) 431
AciI CCGC 1 cut(s) 456
AclI AACGTT 1 cut(s) 45
AclWI GGATC 2 cut(s) 299, 532
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 3 cut(s) 350, 386, 431
AgsI TTSAA 1 cut(s) 306
AjnI CCWGG 3 cut(s) 249, 424, 430
AluBI AGCT 3 cut(s) 33, 182, 221
AluI AGCT 3 cut(s) 33, 182, 221
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 2 cut(s) 299, 532
AlwNI CAGNNNCTG 1 cut(s) 431
AoxI GGCC 2 cut(s) 247, 427
ApeKI GCWGC 3 cut(s) 179, 221, 500
AspA2I CCTAGG 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 309, 428
AsuHPI GGTGA 2 cut(s) 43, 181
AvaII GGWCC 1 cut(s) 309
AvrII CCTAGG 1 cut(s) 287
BanII GRGCYC 1 cut(s) 203
BbsI GAAGAC 2 cut(s) 255, 270
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 3 cut(s) 191, 208, 487
BciT130I CCWGG 3 cut(s) 251, 426, 432
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 4 cut(s) 38, 288, 347, 553
BisI GCNGC 3 cut(s) 180, 222, 501
BlnI CCTAGG 1 cut(s) 287
BlsI GCNGC 3 cut(s) 181, 223, 502
Bme1390I CCNGG 3 cut(s) 251, 426, 432
Bme18I GGWCC 1 cut(s) 309
BmgT120I GGNCC 2 cut(s) 309, 428
BmiI GGNNCC 1 cut(s) 461
BmrFI CCNGG 3 cut(s) 251, 426, 432
BmsI GCATC 3 cut(s) 202, 224, 456
BpiI GAAGAC 2 cut(s) 255, 270
BpmI CTGGAG 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 408
BsaJI CCNNGG 4 cut(s) 272, 287, 424, 430
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 386, 431
Bse1I ACTGG 3 cut(s) 180, 332, 526
Bse3DI GCAATG 1 cut(s) 171
BseBI CCWGG 3 cut(s) 251, 426, 432
BseDI CCNNGG 4 cut(s) 272, 287, 424, 430
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 3 cut(s) 350, 386, 431
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 3 cut(s) 180, 332, 526
BseRI GAGGAG 1 cut(s) 261
BseXI GCAGC 3 cut(s) 191, 208, 487
BseYI CCCAGC 2 cut(s) 182, 393
BshFI GGCC 2 cut(s) 249, 429
BsiHKAI GWGCWC 1 cut(s) 496
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 3 cut(s) 350, 386, 431
BsmAI GTCTC 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 2 cut(s) 249, 429
Bso31I GGTCTC 1 cut(s) 408
Bsp1286I GDGCHC 2 cut(s) 203, 496
Bsp143I GATC 2 cut(s) 291, 524
BspACI CCGC 1 cut(s) 456
BspANI GGCC 2 cut(s) 249, 429
BspCNI CTCAG 1 cut(s) 215
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 461
BspPI GGATC 2 cut(s) 299, 532
BspTNI GGTCTC 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 171
BsrI ACTGG 3 cut(s) 180, 332, 526
BssECI CCNNGG 4 cut(s) 272, 287, 424, 430
BssMI GATC 2 cut(s) 291, 524
BssT1I CCWWGG 1 cut(s) 287
Bst2UI CCWGG 3 cut(s) 251, 426, 432
Bst4CI ACNGT 1 cut(s) 381
BstAPI GCANNNNNTGC 1 cut(s) 221
BstDEI CTNAG 1 cut(s) 202
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 2 cut(s) 294, 527
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 2 cut(s) 291, 524
BstMWI GCNNNNNNNGC 5 cut(s) 161, 221, 453, 500, 506
BstNI CCWGG 3 cut(s) 251, 426, 432
BstSCI CCNGG 3 cut(s) 249, 424, 430
BstV1I GCAGC 3 cut(s) 191, 208, 487
BstV2I GAAGAC 2 cut(s) 255, 270
BsuRI GGCC 2 cut(s) 249, 429
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 2 cut(s) 173, 519
CaiI CAGNNNCTG 1 cut(s) 431
CciI TCATGA 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 309, 428
Csp6I GTAC 1 cut(s) 377
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviAII CATG 2 cut(s) 108, 209
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 1 cut(s) 202
DpnI GATC 2 cut(s) 293, 526
DpnII GATC 2 cut(s) 291, 524
DrdI GACNNNNNNGTC 1 cut(s) 274
DseDI GACNNNNNNGTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 203
Eco31I GGTCTC 1 cut(s) 408
Eco47I GGWCC 1 cut(s) 309
EcoO109I RGGNCCY 1 cut(s) 428
EcoRII CCWGG 3 cut(s) 249, 424, 430
EcoT14I CCWWGG 1 cut(s) 287
EcoT22I ATGCAT 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 2 cut(s) 111, 212
FaiI YATR 6 cut(s) 14, 109, 210, 360, 366, 372
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 107, 208
FauI CCCGC 1 cut(s) 449
Fnu4HI GCNGC 3 cut(s) 180, 222, 501
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 3 cut(s) 180, 222, 501
FspBI CTAG 4 cut(s) 38, 288, 347, 553
GluI GCNGC 3 cut(s) 180, 222, 501
GsaI CCCAGC 2 cut(s) 186, 397
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 2 cut(s) 249, 429
Hin1II CATG 2 cut(s) 111, 212
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HinfI GANTC 3 cut(s) 82, 268, 516
HphI GGTGA 2 cut(s) 43, 181
Hpy166II GTNNAC 1 cut(s) 192
Hpy188I TCNGA 1 cut(s) 389
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 1 cut(s) 192
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 3 cut(s) 111, 215, 509
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 221, 453, 500, 506
HpyF3I CTNAG 1 cut(s) 202
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 2 cut(s) 111, 212
Kzo9I GATC 2 cut(s) 291, 524
Lsp1109I GCAGC 3 cut(s) 191, 208, 487
LweI GCATC 3 cut(s) 202, 224, 456
MaeI CTAG 4 cut(s) 38, 288, 347, 553
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 169
MalI GATC 2 cut(s) 293, 526
MboI GATC 2 cut(s) 291, 524
MboII GAAGA 6 cut(s) 255, 270, 376, 548, 551, 554
MhlI GDGCHC 2 cut(s) 203, 496
MluCI AATT 2 cut(s) 149, 301
MlyI GAGTC 1 cut(s) 262
MmeI TCCRAC 2 cut(s) 223, 487
MnlI CCTC 7 cut(s) 19, 28, 239, 282, 339, 354, 383
Mph1103I ATGCAT 1 cut(s) 217
MspA1I CMGCKG 2 cut(s) 182, 221
MspR9I CCNGG 3 cut(s) 251, 426, 432
MvaI CCWGG 3 cut(s) 251, 426, 432
MwoI GCNNNNNNNGC 5 cut(s) 161, 221, 453, 500, 506
NdeII GATC 2 cut(s) 291, 524
NlaIII CATG 2 cut(s) 111, 212
NlaIV GGNNCC 1 cut(s) 461
NmuCI GTSAC 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 217
PagI TCATGA 1 cut(s) 208
PfeI GAWTC 2 cut(s) 82, 516
PflMI CCANNNNNTGG 1 cut(s) 431
PkrI GCNGC 3 cut(s) 181, 223, 502
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp1406I AACGTT 1 cut(s) 45
Psp6I CCWGG 3 cut(s) 249, 424, 430
PspFI CCCAGC 2 cut(s) 182, 393
PspGI CCWGG 3 cut(s) 249, 424, 430
PspN4I GGNNCC 1 cut(s) 461
PspPI GGNCC 2 cut(s) 309, 428
PstNI CAGNNNCTG 1 cut(s) 431
PvuII CAGCTG 2 cut(s) 182, 221
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SatI GCNGC 3 cut(s) 180, 222, 501
Sau3AI GATC 2 cut(s) 291, 524
Sau96I GGNCC 2 cut(s) 309, 428
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 3 cut(s) 251, 426, 432
SduI GDGCHC 2 cut(s) 203, 496
SetI ASST 5 cut(s) 35, 48, 184, 223, 346
SfaNI GCATC 3 cut(s) 202, 224, 456
SinI GGWCC 1 cut(s) 309
Sse9I AATT 2 cut(s) 149, 301
SsiI CCGC 1 cut(s) 456
SspI AATATT 1 cut(s) 117
SspMI CTAG 4 cut(s) 38, 288, 347, 553
StyD4I CCNGG 3 cut(s) 249, 424, 430
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 1 cut(s) 381
TaiI ACGT 1 cut(s) 48
TaqI TCGA 1 cut(s) 266
TaqII GACCGA 2 cut(s) 249, 264
TasI AATT 2 cut(s) 149, 301
TfiI GAWTC 2 cut(s) 82, 516
TscAI CASTG 2 cut(s) 180, 526
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 3 cut(s) 179, 221, 500
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 4 cut(s) 91, 138, 197, 402
TspRI CASTG 2 cut(s) 180, 526
Van91I CCANNNNNTGG 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 309
XmaJI CCTAGG 1 cut(s) 287
XspI CTAG 4 cut(s) 38, 288, 347, 553
Zsp2I ATGCAT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.