Rmu_sc0004401.1_g000016

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004401.1
OG0016000
Physical Location & Seq
Reverse (-)
81511 .. 85473
3963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004401.1_g000016.1.cds

Sequence Viewer

Length: 792 bp
atgagatctccgacgacctctttgaggattttcggtgaggttttcaggaaagtctggtccgcgattgccggtgataggaatccggtagccggtgaccagaatctaaagatcggtgacaggaattcggcggctggtgactggaatctggcgcccggtgacgggaatccaacaacaggtgaccggaatccgatgaacagtgacggggctccggcgaagtctcctataagctttcttgcaaacgctctcattccacaaacccacaaacccaaaaccaagtacttcacttcgccatacactatctcatgcaaaagaagctcctcatcagatgatgatcatcagaaaggtctttccagtaatgaaattcccagaaagaatgagaaccaatttgcaaagttggcaatggtgacattggcagctggtgtgttgactctgggctctgttcatgatgcttcagctgccaagagtggtggcagagtaggtggacaggcgttccggtcttcggctcctcggccttcctcccctaggatcaataacaattccaggaccaatatttacatcaatcctcctgttgctccgccattagtcggtggttatggatatggttatggatcaccattctttggtgggtggggttggtctccattttcatttttcgccccaggtcctggtgttgtcgttggcattggtggcggcttccaacttttagctattgctgtgtttcttggtgcaattgccgccgtggcaaggagattcaacgcttcaagtgacgaagaagatgatgattactattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

263

Amino Acids

27.76

Weight (kDa)

9.46

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57345
fragaria_vesca FvH4_4g07780
malus_domestica MD13G1247100.v1.1
pyrus_communis pycom13g21800
rosa_chinensis RchiOBHm_Chr4g0400871
rosa_laevigata RLG00000009171
rosa_multiflora Rmu_sc0004401.1_g000016 Rmu_sc0018346.1_g000005
rosa_roxburghii Rroxscaffold_5G00345750
rosa_rugosa Rorug04G0026500
rosa_samantha Rh4AG104500 Rh4BG099700 Rh4CG112900 Rh4DG096600
rosa_wichuraiana Rw4G008460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 148
AccB7I CCANNNNNTGG 2 cut(s) 620, 665
AccII CGCG 1 cut(s) 62
AciI CCGC 5 cut(s) 60, 128, 575, 690, 735
AclWI GGATC 2 cut(s) 533, 616
AcsI RAATTY 2 cut(s) 121, 360
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 1 cut(s) 278
AgsI TTSAA 2 cut(s) 754, 762
AjnI CCWGG 3 cut(s) 539, 658, 664
AluBI AGCT 5 cut(s) 228, 315, 416, 455, 707
AluI AGCT 5 cut(s) 228, 315, 416, 455, 707
Alw26I GTCTC 2 cut(s) 222, 642
AlwI GGATC 2 cut(s) 533, 616
AlwNI CAGNNNCTG 1 cut(s) 665
AoxI GGCC 1 cut(s) 509
ApeKI GCWGC 2 cut(s) 413, 455
ApoI RAATTY 2 cut(s) 121, 360
ArsI GACNNNNNNTTYG 1 cut(s) 36
AspA2I CCTAGG 1 cut(s) 521
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 3 cut(s) 57, 543, 662
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 9 cut(s) 47, 83, 104, 125, 146, 167, 188, 415, 603
AvaII GGWCC 3 cut(s) 57, 543, 662
AvrII CCTAGG 1 cut(s) 521
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 2 cut(s) 208, 437
BbsI GAAGAC 1 cut(s) 489
BbvI GCAGC 2 cut(s) 425, 442
BceAI ACGGC 1 cut(s) 722
BciT130I CCWGG 3 cut(s) 541, 660, 666
BclI TGATCA 1 cut(s) 331
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 2 cut(s) 222, 642
BfaI CTAG 1 cut(s) 522
BfoI RGCGCY 1 cut(s) 152
BglI GCCNNNNNGGC 1 cut(s) 740
BglII AGATCT 1 cut(s) 5
BisI GCNGC 5 cut(s) 129, 414, 456, 691, 735
BlnI CCTAGG 1 cut(s) 521
BlsI GCNGC 5 cut(s) 130, 415, 457, 692, 736
BmcAI AGTACT 1 cut(s) 278
Bme1390I CCNGG 4 cut(s) 153, 541, 660, 666
Bme18I GGWCC 3 cut(s) 57, 543, 662
BmgT120I GGNCC 3 cut(s) 57, 543, 662
BmiI GGNNCC 3 cut(s) 150, 207, 504
BmrFI CCNGG 4 cut(s) 153, 541, 660, 666
BmsI GCATC 1 cut(s) 436
BpiI GAAGAC 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 153
BsaBI GATNNNNATC 3 cut(s) 78, 330, 333
BsaHI GRCGYC 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 642
BsaJI CCNNGG 4 cut(s) 506, 521, 658, 738
BsaWI WCCGGW 3 cut(s) 82, 180, 492
Bse118I RCCGGY 2 cut(s) 68, 89
Bse1I ACTGG 2 cut(s) 143, 351
Bse3DI GCAATG 1 cut(s) 405
Bse8I GATNNNNATC 3 cut(s) 78, 330, 333
BseBI CCWGG 3 cut(s) 541, 660, 666
BseDI CCNNGG 4 cut(s) 506, 521, 658, 738
BseJI GATNNNNATC 3 cut(s) 78, 330, 333
BseMI GCAATG 1 cut(s) 405
BseNI ACTGG 2 cut(s) 143, 351
BseRI GAGGAG 2 cut(s) 307, 495
BseXI GCAGC 2 cut(s) 425, 442
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 1 cut(s) 511
BshNI GGYRCC 1 cut(s) 148
BsiSI CCGG 7 cut(s) 69, 83, 90, 153, 181, 209, 493
BsmAI GTCTC 2 cut(s) 222, 642
BsnI GGCC 1 cut(s) 511
Bso31I GGTCTC 1 cut(s) 642
Bsp1286I GDGCHC 2 cut(s) 208, 437
Bsp143I GATC 5 cut(s) 5, 108, 331, 525, 608
BspACI CCGC 5 cut(s) 60, 128, 575, 690, 735
BspANI GGCC 1 cut(s) 511
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 3 cut(s) 150, 207, 504
BspPI GGATC 2 cut(s) 533, 616
BspT107I GGYRCC 1 cut(s) 148
BspTNI GGTCTC 1 cut(s) 642
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 2 cut(s) 68, 89
BsrI ACTGG 2 cut(s) 143, 351
BssAI RCCGGY 2 cut(s) 68, 89
BssECI CCNNGG 4 cut(s) 506, 521, 658, 738
BssMI GATC 5 cut(s) 5, 108, 331, 525, 608
BssNI GRCGYC 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 521
Bst2UI CCWGG 3 cut(s) 541, 660, 666
Bst4CI ACNGT 1 cut(s) 197
BstACI GRCGYC 1 cut(s) 149
BstDSI CCRYGG 1 cut(s) 738
BstEII GGTNACC 2 cut(s) 92, 176
BstENI CCTNNNNNAGG 2 cut(s) 22, 520
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 5 cut(s) 8, 111, 334, 528, 611
BstMAI GTCTC 2 cut(s) 222, 642
BstMBI GATC 5 cut(s) 5, 108, 331, 525, 608
BstMWI GCNNNNNNNGC 6 cut(s) 312, 395, 455, 687, 734, 740
BstNI CCWGG 3 cut(s) 541, 660, 666
BstPI GGTNACC 2 cut(s) 92, 176
BstSCI CCNGG 4 cut(s) 151, 539, 658, 664
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 2 cut(s) 425, 442
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BsuRI GGCC 1 cut(s) 511
BtgI CCRYGG 1 cut(s) 738
BtsIMutI CAGTG 1 cut(s) 202
CaiI CAGNNNCTG 1 cut(s) 665
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 151
Cfr10I RCCGGY 2 cut(s) 68, 89
Cfr13I GGNCC 3 cut(s) 57, 543, 662
Csp6I GTAC 1 cut(s) 277
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 2 cut(s) 303, 443
CviQI GTAC 1 cut(s) 277
DinI GGCGCC 1 cut(s) 150
DpnI GATC 5 cut(s) 7, 110, 333, 527, 610
DpnII GATC 5 cut(s) 5, 108, 331, 525, 608
EciI GGCGGA 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 521
Eco24I GRGCYC 2 cut(s) 208, 437
Eco31I GGTCTC 1 cut(s) 642
Eco47I GGWCC 3 cut(s) 57, 543, 662
Eco57I CTGAAG 1 cut(s) 435
Eco91I GGTNACC 2 cut(s) 92, 176
EcoNI CCTNNNNNAGG 2 cut(s) 22, 520
EcoO109I RGGNCCY 1 cut(s) 662
EcoO65I GGTNACC 2 cut(s) 92, 176
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 539, 658, 664
EcoT14I CCWWGG 1 cut(s) 521
EcoT38I GRGCYC 2 cut(s) 208, 437
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 521
FaeI CATG 2 cut(s) 306, 446
FaiI YATR 7 cut(s) 224, 292, 304, 444, 594, 600, 606
FatI CATG 2 cut(s) 302, 442
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 5 cut(s) 129, 414, 456, 691, 735
FriOI GRGCYC 2 cut(s) 208, 437
Fsp4HI GCNGC 5 cut(s) 129, 414, 456, 691, 735
FspBI CTAG 1 cut(s) 522
GlaI GCGC 1 cut(s) 150
GluI GCNGC 5 cut(s) 129, 414, 456, 691, 735
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 1 cut(s) 511
HapII CCGG 7 cut(s) 69, 83, 90, 153, 181, 209, 493
HhaI GCGC 1 cut(s) 151
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 2 cut(s) 306, 446
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HindIII AAGCTT 1 cut(s) 226
HinfI GANTC 7 cut(s) 79, 100, 142, 163, 184, 427, 750
HpaII CCGG 7 cut(s) 69, 83, 90, 153, 181, 209, 493
HphI GGTGA 9 cut(s) 47, 83, 104, 125, 146, 167, 188, 415, 603
Hpy166II GTNNAC 2 cut(s) 426, 482
Hpy188I TCNGA 4 cut(s) 12, 189, 325, 339
Hpy188III TCNNGA 2 cut(s) 46, 443
Hpy8I GTNNAC 2 cut(s) 426, 482
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 1 cut(s) 522
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 4 cut(s) 236, 306, 389, 728
HpyF10VI GCNNNNNNNGC 6 cut(s) 312, 395, 455, 687, 734, 740
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 2 cut(s) 306, 446
HspAI GCGC 1 cut(s) 149
KasI GGCGCC 1 cut(s) 148
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 5 cut(s) 5, 108, 331, 525, 608
LmnI GCTCC 4 cut(s) 211, 320, 508, 577
Lsp1109I GCAGC 2 cut(s) 425, 442
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 522
MaeIII GTNAC 8 cut(s) 92, 113, 134, 155, 176, 197, 403, 764
MalI GATC 5 cut(s) 7, 110, 333, 527, 610
MboI GATC 5 cut(s) 5, 108, 331, 525, 608
MboII GAAGA 3 cut(s) 489, 782, 785
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 1 cut(s) 5
MhlI GDGCHC 2 cut(s) 208, 437
MluCI AATT 5 cut(s) 121, 360, 383, 535, 729
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 3 cut(s) 35, 191, 721
MnlI CCTC 7 cut(s) 18, 28, 31, 328, 516, 526, 573
MseI TTAA 1 cut(s) 790
MspA1I CMGCKG 2 cut(s) 416, 455
MspI CCGG 7 cut(s) 69, 83, 90, 153, 181, 209, 493
MspR9I CCNGG 4 cut(s) 153, 541, 660, 666
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 3 cut(s) 541, 660, 666
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 6 cut(s) 312, 395, 455, 687, 734, 740
NarI GGCGCC 1 cut(s) 149
NciI CCSGG 1 cut(s) 153
NdeII GATC 5 cut(s) 5, 108, 331, 525, 608
NlaIII CATG 2 cut(s) 306, 446
NlaIV GGNNCC 3 cut(s) 150, 207, 504
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 8 cut(s) 92, 113, 134, 155, 176, 197, 403, 764
PagI TCATGA 1 cut(s) 442
PfeI GAWTC 6 cut(s) 79, 100, 142, 163, 184, 750
PflMI CCANNNNNTGG 2 cut(s) 620, 665
PfoI TCCNGGA 1 cut(s) 539
PkrI GCNGC 5 cut(s) 130, 415, 457, 692, 736
PleI GAGTC 1 cut(s) 421
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 421
PpuMI RGGWCCY 1 cut(s) 662
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 3 cut(s) 539, 658, 664
PspEI GGTNACC 2 cut(s) 92, 176
PspGI CCWGG 3 cut(s) 539, 658, 664
PspN4I GGNNCC 3 cut(s) 150, 207, 504
PspPI GGNCC 3 cut(s) 57, 543, 662
PspPPI RGGWCCY 1 cut(s) 662
PstNI CAGNNNCTG 1 cut(s) 665
PsuI RGATCY 1 cut(s) 5
PvuII CAGCTG 2 cut(s) 416, 455
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SaqAI TTAA 1 cut(s) 790
SatI GCNGC 5 cut(s) 129, 414, 456, 691, 735
Sau3AI GATC 5 cut(s) 5, 108, 331, 525, 608
Sau96I GGNCC 3 cut(s) 57, 543, 662
ScaI AGTACT 1 cut(s) 278
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 4 cut(s) 153, 541, 660, 666
SduI GDGCHC 2 cut(s) 208, 437
SfaNI GCATC 1 cut(s) 436
SfoI GGCGCC 1 cut(s) 150
SinI GGWCC 3 cut(s) 57, 543, 662
Sse9I AATT 5 cut(s) 121, 360, 383, 535, 729
SsiI CCGC 5 cut(s) 60, 128, 575, 690, 735
SspDI GGCGCC 1 cut(s) 148
SspI AATATT 1 cut(s) 550
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 4 cut(s) 151, 539, 658, 664
StyI CCWWGG 1 cut(s) 521
TaaI ACNGT 1 cut(s) 197
TasI AATT 5 cut(s) 121, 360, 383, 535, 729
TatI WGTACW 1 cut(s) 276
TauI GCSGC 3 cut(s) 131, 693, 737
TfiI GAWTC 6 cut(s) 79, 100, 142, 163, 184, 750
Tru1I TTAA 1 cut(s) 790
Tru9I TTAA 1 cut(s) 790
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 8 cut(s) 92, 113, 134, 155, 176, 197, 403, 764
TseI GCWGC 2 cut(s) 413, 455
Tsp45I GTSAC 8 cut(s) 92, 113, 134, 155, 176, 197, 403, 764
TspDTI ATGAA 4 cut(s) 206, 372, 431, 636
TspRI CASTG 1 cut(s) 202
Van91I CCANNNNNTGG 2 cut(s) 620, 665
VpaK11BI GGWCC 3 cut(s) 57, 543, 662
XagI CCTNNNNNAGG 2 cut(s) 22, 520
XapI RAATTY 2 cut(s) 121, 360
XmaJI CCTAGG 1 cut(s) 521
XspI CTAG 1 cut(s) 522
ZrmI AGTACT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.