Rh4AG104500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016000
Physical Location & Seq
Reverse (-)
22399892 .. 22403160
3269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG104500.1

Sequence Viewer

Length: 576 bp
ATGACTGTGGCCTCATCCCAACAGAGCTTTCTTGCAAACGCTCTCATTCCACAAACCCACAAACCCAAAACCAAGTTCTTCACTTCACCATACACTATCTCATGCAAAAGAAGCTCCTCATCAGATGATGATCATCAGAAAGGTCTTTCCAGAAAGAATGAGAACCAATTTGCAAAGTTGGCAATGGTGACATTGGCAGCTGGTGTGTTGACTCTGGGCTCTGTTCATGATGCTTCAGCTGCCAAGAGTGGTGGCAGAGTAGGTGGACAGGCGTTCCGGTCTTCGGCTCCTCGGCCTTCCTCCCCTAGGATCAATAACAATTCCAGGACCAATATTTACATCAATCCTCCTGTTGCTCCGCCATTAGTCGGTGGTTATGGATATGGTTATGGATCACCATTCTTTGGTGGGTGGGGTTGGTCTCCATTTTCATTTTTCGCCCCAGGTCCTGGTGTTGTCGTTGGCATTGGTGGCGGCTTCCAACTGTTAGCTATTGCTGTGTTTCTTGGTGCAATTGCCGCCGTGGCAAGGAGATTCAACGCTTCAAGAGACGAAGAAGATGATGATTACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

191

Amino Acids

20.21

Weight (kDa)

9.71

Isoelectric Point (pI)

56.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57345
fragaria_vesca FvH4_4g07780
malus_domestica MD13G1247100.v1.1
pyrus_communis pycom13g21800
rosa_chinensis RchiOBHm_Chr4g0400871
rosa_laevigata RLG00000009171
rosa_multiflora Rmu_sc0004401.1_g000016 Rmu_sc0018346.1_g000005
rosa_roxburghii Rroxscaffold_5G00345750
rosa_rugosa Rorug04G0026500
rosa_samantha Rh4AG104500 Rh4BG099700 Rh4CG112900 Rh4DG096600
rosa_wichuraiana Rw4G008460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 404, 449
AciI CCGC 3 cut(s) 359, 474, 519
AclWI GGATC 2 cut(s) 317, 400
AcuI CTGAAG 1 cut(s) 219
AfiI CCNNNNNNNGG 6 cut(s) 283, 306, 368, 404, 449, 528
AgsI TTSAA 2 cut(s) 538, 546
AjnI CCWGG 3 cut(s) 323, 442, 448
AjuI GAANNNNNNNTTGG 4 cut(s) 12, 44, 59, 91
AluBI AGCT 5 cut(s) 27, 114, 200, 239, 491
AluI AGCT 5 cut(s) 27, 114, 200, 239, 491
Alw26I GTCTC 2 cut(s) 426, 543
AlwI GGATC 2 cut(s) 317, 400
AlwNI CAGNNNCTG 1 cut(s) 449
AoxI GGCC 2 cut(s) 9, 293
ApeKI GCWGC 2 cut(s) 197, 239
AspA2I CCTAGG 1 cut(s) 305
AspS9I GGNCC 2 cut(s) 327, 446
AsuHPI GGTGA 3 cut(s) 78, 199, 387
AvaII GGWCC 2 cut(s) 327, 446
AvrII CCTAGG 1 cut(s) 305
BanII GRGCYC 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 209, 226
BceAI ACGGC 1 cut(s) 506
BciT130I CCWGG 3 cut(s) 325, 444, 450
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 2 cut(s) 426, 543
BfaI CTAG 1 cut(s) 306
BglI GCCNNNNNGGC 1 cut(s) 524
BisI GCNGC 4 cut(s) 198, 240, 475, 519
BlnI CCTAGG 1 cut(s) 305
BlsI GCNGC 4 cut(s) 199, 241, 476, 520
Bme1390I CCNGG 3 cut(s) 325, 444, 450
Bme18I GGWCC 2 cut(s) 327, 446
BmgT120I GGNCC 2 cut(s) 327, 446
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 3 cut(s) 325, 444, 450
BmsI GCATC 1 cut(s) 220
BpiI GAAGAC 1 cut(s) 273
BsaBI GATNNNNATC 2 cut(s) 129, 132
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 4 cut(s) 290, 305, 442, 522
BsaWI WCCGGW 1 cut(s) 276
Bsc4I CCNNNNNNNGG 6 cut(s) 283, 306, 368, 404, 449, 528
Bse3DI GCAATG 1 cut(s) 189
Bse8I GATNNNNATC 2 cut(s) 129, 132
BseBI CCWGG 3 cut(s) 325, 444, 450
BseDI CCNNGG 4 cut(s) 290, 305, 442, 522
BseGI GGATG 1 cut(s) 14
BseJI GATNNNNATC 2 cut(s) 129, 132
BseLI CCNNNNNNNGG 6 cut(s) 283, 306, 368, 404, 449, 528
BseMI GCAATG 1 cut(s) 189
BseRI GAGGAG 2 cut(s) 106, 279
BseXI GCAGC 2 cut(s) 209, 226
BshFI GGCC 2 cut(s) 11, 295
BsiSI CCGG 1 cut(s) 277
BslI CCNNNNNNNGG 6 cut(s) 283, 306, 368, 404, 449, 528
BsmAI GTCTC 2 cut(s) 426, 543
BsmBI CGTCTC 1 cut(s) 543
BsnI GGCC 2 cut(s) 11, 295
Bso31I GGTCTC 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 3 cut(s) 130, 309, 392
BspACI CCGC 3 cut(s) 359, 474, 519
BspANI GGCC 2 cut(s) 11, 295
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 1 cut(s) 288
BspPI GGATC 2 cut(s) 317, 400
BspTNI GGTCTC 1 cut(s) 426
BsrDI GCAATG 1 cut(s) 189
BssECI CCNNGG 4 cut(s) 290, 305, 442, 522
BssMI GATC 3 cut(s) 130, 309, 392
BssT1I CCWWGG 1 cut(s) 305
Bst2UI CCWGG 3 cut(s) 325, 444, 450
Bst4CI ACNGT 2 cut(s) 7, 486
BstDSI CCRYGG 1 cut(s) 522
BstENI CCTNNNNNAGG 1 cut(s) 304
BstF5I GGATG 1 cut(s) 14
BstKTI GATC 3 cut(s) 133, 312, 395
BstMAI GTCTC 2 cut(s) 426, 543
BstMBI GATC 3 cut(s) 130, 309, 392
BstMWI GCNNNNNNNGC 6 cut(s) 111, 179, 239, 471, 518, 524
BstNI CCWGG 3 cut(s) 325, 444, 450
BstSCI CCNGG 3 cut(s) 323, 442, 448
BstV1I GCAGC 2 cut(s) 209, 226
BstV2I GAAGAC 1 cut(s) 273
BsuRI GGCC 2 cut(s) 11, 295
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 1 cut(s) 14
CaiI CAGNNNCTG 1 cut(s) 449
CciI TCATGA 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 327, 446
CspCI CAANNNNNGTGG 2 cut(s) 232, 267
CviAII CATG 2 cut(s) 102, 227
DpnI GATC 3 cut(s) 132, 311, 394
DpnII GATC 3 cut(s) 130, 309, 392
EciI GGCGGA 1 cut(s) 348
Eco130I CCWWGG 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 426
Eco47I GGWCC 2 cut(s) 327, 446
Eco57I CTGAAG 1 cut(s) 219
EcoNI CCTNNNNNAGG 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 3 cut(s) 323, 442, 448
EcoT14I CCWWGG 1 cut(s) 305
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 305
Esp3I CGTCTC 1 cut(s) 543
FaeI CATG 2 cut(s) 105, 230
FaiI YATR 6 cut(s) 91, 103, 228, 378, 384, 390
FatI CATG 2 cut(s) 101, 226
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 4 cut(s) 198, 240, 475, 519
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 4 cut(s) 198, 240, 475, 519
FspBI CTAG 1 cut(s) 306
GluI GCNGC 4 cut(s) 198, 240, 475, 519
HaeIII GGCC 2 cut(s) 11, 295
HapII CCGG 1 cut(s) 277
Hin1II CATG 2 cut(s) 105, 230
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 2 cut(s) 211, 534
HpaII CCGG 1 cut(s) 277
HphI GGTGA 3 cut(s) 78, 199, 387
Hpy166II GTNNAC 2 cut(s) 210, 266
Hpy188I TCNGA 2 cut(s) 124, 138
Hpy188III TCNNGA 3 cut(s) 150, 227, 546
Hpy8I GTNNAC 2 cut(s) 210, 266
HpyAV CCTTC 1 cut(s) 306
HpyCH4III ACNGT 2 cut(s) 7, 486
HpyCH4V TGCA 4 cut(s) 35, 105, 173, 512
HpyF10VI GCNNNNNNNGC 6 cut(s) 111, 179, 239, 471, 518, 524
Hsp92II CATG 2 cut(s) 105, 230
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 3 cut(s) 130, 309, 392
LmnI GCTCC 3 cut(s) 119, 292, 361
Lsp1109I GCAGC 2 cut(s) 209, 226
LweI GCATC 1 cut(s) 220
MaeI CTAG 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 187
MalI GATC 3 cut(s) 132, 311, 394
MboI GATC 3 cut(s) 130, 309, 392
MboII GAAGA 4 cut(s) 70, 273, 566, 569
MfeI CAATTG 1 cut(s) 513
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 3 cut(s) 167, 319, 513
MlyI GAGTC 1 cut(s) 205
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 5 cut(s) 22, 127, 300, 310, 357
MseI TTAA 1 cut(s) 574
MspA1I CMGCKG 2 cut(s) 200, 239
MspI CCGG 1 cut(s) 277
MspR9I CCNGG 3 cut(s) 325, 444, 450
MunI CAATTG 1 cut(s) 513
MvaI CCWGG 3 cut(s) 325, 444, 450
MwoI GCNNNNNNNGC 6 cut(s) 111, 179, 239, 471, 518, 524
NdeII GATC 3 cut(s) 130, 309, 392
NlaIII CATG 2 cut(s) 105, 230
NlaIV GGNNCC 1 cut(s) 288
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 1 cut(s) 187
PagI TCATGA 1 cut(s) 226
PfeI GAWTC 1 cut(s) 534
PflMI CCANNNNNTGG 2 cut(s) 404, 449
PfoI TCCNGGA 1 cut(s) 323
PkrI GCNGC 4 cut(s) 199, 241, 476, 520
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PpuMI RGGWCCY 1 cut(s) 446
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 3 cut(s) 323, 442, 448
PspGI CCWGG 3 cut(s) 323, 442, 448
PspN4I GGNNCC 1 cut(s) 288
PspPI GGNCC 2 cut(s) 327, 446
PspPPI RGGWCCY 1 cut(s) 446
PstNI CAGNNNCTG 1 cut(s) 449
PvuII CAGCTG 2 cut(s) 200, 239
SaqAI TTAA 1 cut(s) 574
SatI GCNGC 4 cut(s) 198, 240, 475, 519
Sau3AI GATC 3 cut(s) 130, 309, 392
Sau96I GGNCC 2 cut(s) 327, 446
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 3 cut(s) 325, 444, 450
SduI GDGCHC 1 cut(s) 221
SetI ASST 8 cut(s) 29, 116, 145, 202, 241, 265, 448, 493
SfaNI GCATC 1 cut(s) 220
SinI GGWCC 2 cut(s) 327, 446
Sse9I AATT 3 cut(s) 167, 319, 513
SsiI CCGC 3 cut(s) 359, 474, 519
SspI AATATT 1 cut(s) 334
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 3 cut(s) 323, 442, 448
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 2 cut(s) 7, 486
TasI AATT 3 cut(s) 167, 319, 513
TauI GCSGC 2 cut(s) 477, 521
TfiI GAWTC 1 cut(s) 534
Tru1I TTAA 1 cut(s) 574
Tru9I TTAA 1 cut(s) 574
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 2 cut(s) 197, 239
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 215, 420
Van91I CCANNNNNTGG 2 cut(s) 404, 449
VpaK11BI GGWCC 2 cut(s) 327, 446
XagI CCTNNNNNAGG 1 cut(s) 304
XmaJI CCTAGG 1 cut(s) 305
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.