MD13G1249400.v1.1

Adagio protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004937
Physical Location & Seq
Forward (+)
26495237 .. 26500705
5469 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1249400.v1.1.491

Sequence Viewer

Length: 1872 bp
ATGGAGTGGGATAGCAATTCCGATCTGAGCTCCGAGGAGGAGGAGGACGAGGAAGGGAGCTTGATTTTCCCTGACGACGACGACGTTTCTGCCCCTGGTTCGCTTCCGCTTCCGTTCCCCGTCGAGAATTTGCTCCAAACGGCGCCGTGTGGCTTCGTTGTGACGGACGCTCTCGACCCTGACCACCCCATCATCTACGTCAACACTGTGTTTGAAATGGTCTCGGGGTACCGTGCCGAGGAGGTTCTTGGTCGCAATTGCCGGTTTTTGCAATATAGAGGACCATTTGCAAAAAGAAGGCATCCACTAGTAGACTCTGCAGTAGTTGCAGAAATTAAAAGATGTCTGGAGGAGGGTATTGAATTCCAAGGTGAGTTATTAAACTTTAGGAAAGATGGATCTCCAATGATGAATAGATTGCGGCTGACACCAATCTATGGGGATGATGATGTGATAACTCACATCATCGGGATTCAGTTCTTCACGGAGGCAGATATTGATCTAGGTCCCGTGCCTGGCACTTCAATCAAGGAGTCAGCAAAATTTTCTGATCGGTTTCGTTCTTGTCTTTCTGGTTTCCGTCCTGCTGCCTTCCTGGGGGAGCGGAATATGTGTCGTGGGGTTTGTGGAATGTTGCAATTGAGTGATGAGGTACTTGCTCTCAAGATTCTATCGAAATTGGCACCTAGAGACATTGCATCTATTGGTTCAGTCTGTAGGCGGCTGTATGAGCTTTCGAAGAATGAAGACCTTTGGAGAATGGTCTGCCAAAATGCATGGGGTAGTGAAACAACTCGGGTTTTAGAGGCTGTGCCTGGTGCGAAGAGGCTAGGTTGGGGTAGGTTGGCACGGGAGCTGACTACTCTTGAAGCAGCGACATGGAGAAAGGTGACTGTTGGAGGTTCTGTTGAACCCTCACGCTGTAACTTTAGTGCTTGTGCAGTTGGAAATCGTGTTGTCCTTTTTGGTGGTGAAGGGGTTAATATGCAACCGATGAATGACACCTTCATATTGGATCTAAATGCCAGTAACCCAGAGTGGCAGCATGTCCAAGTCAGCTCTCCTCCACCTGGTCGTTGGGGCCATACACTATCTTGTGTGAATGGGTCCCATTTGGTGGTATTTGGAGGCTGTGGAAGGCAGGGCTTGCTAAATGATGTGTTCGTACTGGATTTGGACGCAAAGCCTCCAACTTGGCGTGAAATATCTGGGTTGGCTCCTCCACTTCCTAGATCATGGCACAGTTCCTGCACTCTTGATGGCACCAAGTTGATAGTTTCAGGTGGCTGTGCAGATTCTGGAGTACTCCTTAGTGATACTTTTTTGCTTGATCTCTCAATGGAGAAACCTGTGTGGAGAGAGATACCAGTAGCATGGAAGCCCCCTTCAAGATTGGGCCACACCCTGTCTGTATATGGTGGTAGAAAAATTTTGATGTTTGGGGGCCTGGCCAAAAGTGGTCCACTTCGTTTTCGTTCCAGCGATGTGTTCACAATGGATCTGAGTGAGGATGAACCGTGTTGGAGGTGTGTTACAGGGAGTGGAATGCCCGGTTCTGGAAATCCAGGGGGCATGGCTCCTCCTCCTAGACTGGATCATGTAGCTGTGAGCCTTCCAGGTGGGAGAATACTGATCTTTGGAGGGTCTGTTGCTGGTCTTCACTCTGCGTCACAACTTTATCTCTTAGATCCAATGGATGAAAAACCTACATGGAGGATATTGAATGTACCTGGGCGGCCCCCAAGATTCGCCTGGGGACATAGTACTTGTGTTGTTGGAGGGACAAGGGCAATTGTCCTTGGTGGTCAAACTGGAGAAGAGTGGATGTTAAGTGAACTCCATGAGCTTTCACTGGCAAACTCTGTAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
F-box-like Kelch_2 Kelch_4 PAS_9

Protein Analysis

624

Amino Acids

68.02

Weight (kDa)

5.34

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 42 - 158 6.8e-08 PAS fold
PAS_9 PF13426 49 - 159 7.4e-16 PAS domain
PAS_3 PF08447 64 - 158 9.4e-07 PAS fold
F-box-like PF12937 219 - 257 4.3e-09 F-box-like
Kelch_KLHDC2_KLHL20_DRC7 PF24681 288 - 482 2e-37 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 289 - 447 6.1e-27 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 289 - 430 1.6e-10 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 306 - 501 8.1e-09 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_HCF PF13854 345 - 603 7.5e-14 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 448 - 619 2.8e-15 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 451 - 620 1.7e-18 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2 PF07646 531 - 578 4.1e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 228
AccB1I GGYRCC 4 cut(s) 142, 228, 682, 1262
AccB7I CCANNNNNTGG 2 cut(s) 437, 1117
AccBSI CCGCTC 1 cut(s) 604
AccI GTMKAC 1 cut(s) 312
AciI CCGC 5 cut(s) 107, 421, 604, 721, 1735
AclWI GGATC 5 cut(s) 406, 1023, 1506, 1602, 1682
AcoI YGGCCR 1 cut(s) 1449
AcsI RAATTY 4 cut(s) 127, 362, 542, 1428
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 6 cut(s) 230, 654, 1167, 1305, 1728, 1765
AfiI CCNNNNNNNGG 7 cut(s) 238, 437, 515, 597, 1070, 1117, 1556
AgsI TTSAA 7 cut(s) 215, 362, 525, 869, 911, 1389, 1723
AhlI ACTAGT 1 cut(s) 307
AluBI AGCT 7 cut(s) 30, 60, 733, 856, 1059, 1604, 1846
AluI AGCT 7 cut(s) 30, 60, 733, 856, 1059, 1604, 1846
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 226, 684
AlwI GGATC 5 cut(s) 406, 1023, 1506, 1602, 1682
AlwNI CAGNNNCTG 2 cut(s) 1248, 1298
Ama87I CYCGRG 2 cut(s) 223, 795
AoxI GGCC 5 cut(s) 1081, 1396, 1444, 1449, 1736
ApeKI GCWGC 3 cut(s) 587, 872, 1042
ApoI RAATTY 4 cut(s) 127, 362, 542, 1428
Asp718I GGTACC 1 cut(s) 228
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 8 cut(s) 281, 506, 1081, 1107, 1396, 1444, 1460, 1737
AsuC2I CCSGG 1 cut(s) 1551
AsuHPI GGTGA 3 cut(s) 383, 901, 983
AsuII TTCGAA 1 cut(s) 737
AvaI CYCGRG 2 cut(s) 223, 795
AvaII GGWCC 4 cut(s) 281, 506, 1107, 1460
BalI TGGCCA 1 cut(s) 1451
BanI GGYRCC 4 cut(s) 142, 228, 682, 1262
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 2 cut(s) 753, 1649
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 3 cut(s) 574, 884, 1054
BccI CCATC 3 cut(s) 197, 389, 1253
BceAI ACGGC 2 cut(s) 130, 156
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BcnI CCSGG 1 cut(s) 1551
BcoDI GTCTC 2 cut(s) 226, 684
BcuI ACTAGT 1 cut(s) 307
BfaI CTAG 6 cut(s) 308, 503, 687, 830, 1230, 1587
BfmI CTRYAG 2 cut(s) 318, 715
BfoI RGCGCY 1 cut(s) 146
BisI GCNGC 6 cut(s) 422, 588, 722, 873, 1043, 1736
BlsI GCNGC 6 cut(s) 423, 589, 723, 874, 1044, 1737
BmcAI AGTACT 2 cut(s) 1305, 1765
Bme18I GGWCC 4 cut(s) 281, 506, 1107, 1460
BmeT110I CYCGRG 2 cut(s) 223, 795
BmgT120I GGNCC 8 cut(s) 281, 506, 1081, 1107, 1396, 1444, 1460, 1737
BmsI GCATC 2 cut(s) 310, 707
BpiI GAAGAC 2 cut(s) 753, 1649
BpmI CTGGAG 3 cut(s) 368, 1320, 1833
Bpu14I TTCGAA 1 cut(s) 737
BpuEI CTTGAG 1 cut(s) 647
BpuMI CCSGG 1 cut(s) 1551
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 143
BsaI GGTCTC 1 cut(s) 226
BsaJI CCNNGG 9 cut(s) 33, 94, 237, 367, 595, 1565, 1730, 1752, 1798
BsaXI ACNNNNNCTCC 4 cut(s) 891, 921, 1632, 1662
Bsc4I CCNNNNNNNGG 7 cut(s) 238, 437, 515, 597, 1070, 1117, 1556
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 6 cut(s) 1026, 1173, 1367, 1596, 1816, 1857
Bse3DI GCAATG 1 cut(s) 693
Bse8I GATNNNNATC 1 cut(s) 498
BseDI CCNNGG 9 cut(s) 33, 94, 237, 367, 595, 1565, 1730, 1752, 1798
BseGI GGATG 5 cut(s) 301, 448, 1516, 1702, 1830
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 7 cut(s) 238, 437, 515, 597, 1070, 1117, 1556
BseMI GCAATG 1 cut(s) 693
BseMII CTCAG 2 cut(s) 17, 1493
BseNI ACTGG 6 cut(s) 1026, 1173, 1367, 1596, 1816, 1857
BseRI GAGGAG 9 cut(s) 50, 53, 56, 254, 365, 1053, 1209, 1569, 1572
BseXI GCAGC 3 cut(s) 574, 884, 1054
BsgI GTGCAG 3 cut(s) 960, 1234, 1311
BshFI GGCC 5 cut(s) 1083, 1398, 1446, 1451, 1738
BshNI GGYRCC 4 cut(s) 142, 228, 682, 1262
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 2 cut(s) 223, 795
BsiSI CCGG 2 cut(s) 262, 1551
BslFI GGGAC 4 cut(s) 492, 1093, 1770, 1795
BslI CCNNNNNNNGG 7 cut(s) 238, 437, 515, 597, 1070, 1117, 1556
BsmAI GTCTC 2 cut(s) 226, 684
BsmFI GGGAC 4 cut(s) 492, 1093, 1770, 1795
BsmI GAATGC 1 cut(s) 1551
BsnI GGCC 5 cut(s) 1083, 1398, 1446, 1451, 1738
Bso31I GGTCTC 1 cut(s) 226
BsoBI CYCGRG 2 cut(s) 223, 795
Bsp119I TTCGAA 1 cut(s) 737
Bsp1286I GDGCHC 1 cut(s) 32
BspACI CCGC 5 cut(s) 107, 421, 604, 721, 1735
BspANI GGCC 5 cut(s) 1083, 1398, 1446, 1451, 1738
BspCNI CTCAG 2 cut(s) 18, 1494
BspMAI CTGCAG 1 cut(s) 322
BspPI GGATC 5 cut(s) 406, 1023, 1506, 1602, 1682
BspT104I TTCGAA 1 cut(s) 737
BspT107I GGYRCC 4 cut(s) 142, 228, 682, 1262
BspTNI GGTCTC 1 cut(s) 226
BsrBI CCGCTC 1 cut(s) 604
BsrDI GCAATG 1 cut(s) 693
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 6 cut(s) 1026, 1173, 1367, 1596, 1816, 1857
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 9 cut(s) 33, 94, 237, 367, 595, 1565, 1730, 1752, 1798
BssNI GRCGYC 1 cut(s) 143
BssT1I CCWWGG 2 cut(s) 367, 1798
Bst4CI ACNGT 5 cut(s) 208, 233, 895, 1244, 1518
Bst6I CTCTTC 2 cut(s) 818, 1812
BstACI GRCGYC 1 cut(s) 143
BstAPI GCANNNNNTGC 2 cut(s) 326, 1147
BstBI TTCGAA 1 cut(s) 737
BstC8I GCNNGC 1 cut(s) 1148
BstDEI CTNAG 4 cut(s) 26, 1310, 1502, 1684
BstF5I GGATG 5 cut(s) 301, 448, 1516, 1702, 1830
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 1 cut(s) 145
BstMAI GTCTC 2 cut(s) 226, 684
BstMWI GCNNNNNNNGC 3 cut(s) 326, 730, 1147
BstNSI RCATGY 1 cut(s) 1049
BstSFI CTRYAG 2 cut(s) 318, 715
BstV1I GCAGC 3 cut(s) 574, 884, 1054
BstV2I GAAGAC 2 cut(s) 753, 1649
BstX2I RGATCY 4 cut(s) 398, 1015, 1498, 1687
BstXI CCANNNNNNTGG 1 cut(s) 1374
BstYI RGATCY 4 cut(s) 398, 1015, 1498, 1687
BsuRI GGCC 5 cut(s) 1083, 1398, 1446, 1451, 1738
BtgZI GCGATG 1 cut(s) 1497
BtsCI GGATG 5 cut(s) 301, 448, 1516, 1702, 1830
BtsIMutI CAGTG 2 cut(s) 204, 1850
Cac8I GCNNGC 1 cut(s) 1148
CaiI CAGNNNCTG 2 cut(s) 1248, 1298
CfoI GCGC 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 8 cut(s) 281, 506, 1081, 1107, 1396, 1444, 1460, 1737
CseI GACGC 3 cut(s) 176, 1187, 1656
CsiI ACCWGGT 1 cut(s) 1069
Csp6I GTAC 6 cut(s) 229, 653, 1166, 1304, 1727, 1764
CviAII CATG 9 cut(s) 777, 879, 1046, 1236, 1374, 1573, 1598, 1710, 1841
CviQI GTAC 6 cut(s) 229, 653, 1166, 1304, 1727, 1764
DdeI CTNAG 4 cut(s) 26, 1310, 1502, 1684
DinI GGCGCC 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 1449
Eam1104I CTCTTC 2 cut(s) 818, 1812
EarI CTCTTC 2 cut(s) 818, 1812
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 2 cut(s) 367, 1798
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 226
Eco47I GGWCC 4 cut(s) 281, 506, 1107, 1460
Eco53kI GAGCTC 1 cut(s) 30
Eco88I CYCGRG 2 cut(s) 223, 795
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 3 cut(s) 506, 1107, 1444
EcoRI GAATTC 1 cut(s) 362
EcoT14I CCWWGG 2 cut(s) 367, 1798
EcoT22I ATGCAT 1 cut(s) 778
EcoT38I GRGCYC 1 cut(s) 32
EgeI GGCGCC 1 cut(s) 144
EheI GGCGCC 1 cut(s) 144
ErhI CCWWGG 2 cut(s) 367, 1798
FaeI CATG 9 cut(s) 780, 882, 1049, 1239, 1377, 1576, 1601, 1713, 1844
FaqI GGGAC 4 cut(s) 492, 1093, 1770, 1795
FatI CATG 9 cut(s) 776, 878, 1045, 1235, 1373, 1572, 1597, 1709, 1840
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 6 cut(s) 422, 588, 722, 873, 1043, 1736
FokI GGATG 5 cut(s) 288, 455, 1523, 1709, 1837
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 6 cut(s) 422, 588, 722, 873, 1043, 1736
FspBI CTAG 6 cut(s) 308, 503, 687, 830, 1230, 1587
GlaI GCGC 1 cut(s) 144
GluI GCNGC 6 cut(s) 422, 588, 722, 873, 1043, 1736
GsuI CTGGAG 3 cut(s) 368, 1320, 1833
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 5 cut(s) 1083, 1398, 1446, 1451, 1738
HapII CCGG 2 cut(s) 262, 1551
HgaI GACGC 3 cut(s) 176, 1187, 1656
HhaI GCGC 1 cut(s) 145
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 9 cut(s) 780, 882, 1049, 1239, 1377, 1576, 1601, 1713, 1844
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 6 cut(s) 314, 472, 533, 667, 1295, 1746
HpaII CCGG 2 cut(s) 262, 1551
HphI GGTGA 3 cut(s) 383, 901, 983
Hpy166II GTNNAC 5 cut(s) 202, 313, 1463, 1491, 1835
Hpy188I TCNGA 6 cut(s) 22, 27, 34, 550, 1503, 1871
Hpy8I GTNNAC 5 cut(s) 202, 313, 1463, 1491, 1835
Hpy99I CGWCG 4 cut(s) 80, 83, 86, 125
HpyAV CCTTC 8 cut(s) 47, 291, 601, 968, 1015, 1131, 1395, 1622
HpyCH4III ACNGT 5 cut(s) 208, 233, 895, 1244, 1518
HpyCH4IV ACGT 2 cut(s) 84, 198
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 730, 1147
HpyF3I CTNAG 4 cut(s) 26, 1310, 1502, 1684
HpySE526I ACGT 2 cut(s) 84, 198
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 9 cut(s) 780, 882, 1049, 1239, 1377, 1576, 1601, 1713, 1844
HspAI GCGC 1 cut(s) 143
KasI GGCGCC 1 cut(s) 142
KflI GGGWCCC 1 cut(s) 1107
KpnI GGTACC 1 cut(s) 232
LmnI GCTCC 7 cut(s) 35, 57, 138, 601, 853, 1222, 1582
Lsp1109I GCAGC 3 cut(s) 574, 884, 1054
LweI GCATC 2 cut(s) 310, 707
MabI ACCWGGT 1 cut(s) 1069
MaeI CTAG 6 cut(s) 308, 503, 687, 830, 1230, 1587
MaeII ACGT 2 cut(s) 84, 198
MaeIII GTNAC 6 cut(s) 160, 889, 923, 1028, 1531, 1668
MbiI CCGCTC 1 cut(s) 604
MboII GAAGA 6 cut(s) 472, 751, 758, 835, 1649, 1829
MfeI CAATTG 3 cut(s) 256, 638, 1791
MflI RGATCY 4 cut(s) 398, 1015, 1498, 1687
MhlI GDGCHC 1 cut(s) 32
MlsI TGGCCA 1 cut(s) 1451
MluNI TGGCCA 1 cut(s) 1451
Mly113I GGCGCC 1 cut(s) 143
MlyI GAGTC 2 cut(s) 308, 542
MmeI TCCRAC 5 cut(s) 877, 925, 1214, 1502, 1756
Mox20I TGGCCA 1 cut(s) 1451
Mph1103I ATGCAT 1 cut(s) 778
MscI TGGCCA 1 cut(s) 1451
MseI TTAA 4 cut(s) 336, 380, 981, 1829
Msp20I TGGCCA 1 cut(s) 1451
MspI CCGG 2 cut(s) 262, 1551
MunI CAATTG 3 cut(s) 256, 638, 1791
Mva1269I GAATGC 1 cut(s) 1551
MwoI GCNNNNNNNGC 3 cut(s) 326, 730, 1147
NarI GGCGCC 1 cut(s) 143
NciI CCSGG 1 cut(s) 1551
NlaIII CATG 9 cut(s) 780, 882, 1049, 1239, 1377, 1576, 1601, 1713, 1844
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 3 cut(s) 160, 889, 1668
NsiI ATGCAT 1 cut(s) 778
NspI RCATGY 1 cut(s) 1049
NspV TTCGAA 1 cut(s) 737
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 1551
PfeI GAWTC 4 cut(s) 472, 667, 1295, 1746
PflMI CCANNNNNTGG 2 cut(s) 437, 1117
PkrI GCNGC 6 cut(s) 423, 589, 723, 874, 1044, 1737
PleI GAGTC 2 cut(s) 308, 541
PluTI GGCGCC 1 cut(s) 146
PpsI GAGTC 2 cut(s) 308, 541
PpuMI RGGWCCY 2 cut(s) 506, 1107
Psp124BI GAGCTC 1 cut(s) 32
Psp5II RGGWCCY 2 cut(s) 506, 1107
PspPI GGNCC 8 cut(s) 281, 506, 1081, 1107, 1396, 1444, 1460, 1737
PspPPI RGGWCCY 2 cut(s) 506, 1107
PstI CTGCAG 1 cut(s) 322
PstNI CAGNNNCTG 2 cut(s) 1248, 1298
PsuI RGATCY 4 cut(s) 398, 1015, 1498, 1687
RsaI GTAC 6 cut(s) 230, 654, 1167, 1305, 1728, 1765
RsaNI GTAC 6 cut(s) 229, 653, 1166, 1304, 1727, 1764
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 4 cut(s) 336, 380, 981, 1829
SatI GCNGC 6 cut(s) 422, 588, 722, 873, 1043, 1736
Sau96I GGNCC 8 cut(s) 281, 506, 1081, 1107, 1396, 1444, 1460, 1737
ScaI AGTACT 2 cut(s) 1305, 1765
SchI GAGTC 2 cut(s) 308, 542
SduI GDGCHC 1 cut(s) 32
SexAI ACCWGGT 1 cut(s) 1069
SfaNI GCATC 2 cut(s) 310, 707
SfcI CTRYAG 2 cut(s) 318, 715
SfoI GGCGCC 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 737
SinI GGWCC 4 cut(s) 281, 506, 1107, 1460
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
SpeI ACTAGT 1 cut(s) 307
SsiI CCGC 5 cut(s) 107, 421, 604, 721, 1735
SspDI GGCGCC 1 cut(s) 142
SspMI CTAG 6 cut(s) 308, 503, 687, 830, 1230, 1587
SstI GAGCTC 1 cut(s) 32
StyI CCWWGG 2 cut(s) 367, 1798
TaaI ACNGT 5 cut(s) 208, 233, 895, 1244, 1518
TaiI ACGT 2 cut(s) 87, 201
TaqI TCGA 4 cut(s) 123, 174, 674, 737
TatI WGTACW 2 cut(s) 1303, 1763
TauI GCSGC 3 cut(s) 424, 724, 1738
TfiI GAWTC 4 cut(s) 472, 667, 1295, 1746
Tru1I TTAA 4 cut(s) 336, 380, 981, 1829
Tru9I TTAA 4 cut(s) 336, 380, 981, 1829
TscAI CASTG 2 cut(s) 211, 1857
TseFI GTSAC 3 cut(s) 160, 889, 1668
TseI GCWGC 3 cut(s) 587, 872, 1042
Tsp45I GTSAC 3 cut(s) 160, 889, 1668
TspDTI ATGAA 6 cut(s) 425, 759, 997, 1010, 1527, 1713
TspGWI ACGGA 4 cut(s) 102, 179, 500, 569
TspRI CASTG 2 cut(s) 211, 1857
Van91I CCANNNNNTGG 2 cut(s) 437, 1117
VpaK11BI GGWCC 4 cut(s) 281, 506, 1107, 1460
XapI RAATTY 4 cut(s) 127, 362, 542, 1428
XceI RCATGY 1 cut(s) 1049
XcmI CCANNNNNNNNNTGG 2 cut(s) 1074, 1749
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 6 cut(s) 308, 503, 687, 830, 1230, 1587
ZrmI AGTACT 2 cut(s) 1305, 1765
Zsp2I ATGCAT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.