pycom13g21980

Adagio protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004937
Physical Location & Seq
Forward (+)
19063936 .. 19069062
5127 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21980.1

Sequence Viewer

Length: 1695 bp
ATGGAGTGGGATAGCAATTCCGATCTGAGCTCCGAGGAGGAGGAGGACGAGGATGGGAGCTTGATTTTCCCTGACGACGACGACGTTTCTGCCCCGGGTCCGCTTCCGTTCCCCGTCGAGAATTTGTTCCAAACGGCGCCGTGTGGCTTCGTTGTGACGGACGCTCTCGACCCTGACCACCCCATCATCTACGTCAACACTGTGTTTGAAATGGTCTCGGGGTACCGTGCCGAGGAGGTTCTTGGTCGCAATTGCCGGTTTTTGCAATATAGAGGGCCATTTGCAAAAAGAAGGCATCCACTAGTAGACTCTGCAGTAGTTGCAGAAATTAAAAGATGTCTGGAGGAGGGTATTGAATTCCAAGGCGAGTTATTAAACTTTAGGAAAGATGGATCTCCAATGATGAATAGATTGCGGCTGACACCAATATATGGGGATGATGATGTGATAACTCACGTCATCGGGATTCAGTTCTTCACGGAGGCAGACATTGATCTAGGTCCTGTGCCCGGCACTTCAATCAAGGAGTCAGCAAAATTTTCTGATCGGTTTCGTTCTTGTCTTTCTGCTTTCCGTCCTGCTGCCTTCCTGGGGGAGCGGAATATGTGTCGTGGGGTTTGTGGAATGTTGCAATTGAGTGATGAGGTACTTGCTCTCAAGATTCTATCGAAATTGGCACCTAGAGACATTGCATCTATTGGTTCAGTCTGTAGGCGACTGTATGAGCTTTCGAAGAATGAAGACCTTTGGAGAATGGTCTGCCAAAATGCATGGGGTAGTGAAACAACTCGGGTTTTAGAGGCCGTGCCTGGTGCGAAGAGGCTAGGTTGGGGTAGGTTGGCACGGGAGCTGACTACTCTTGAAGCAGCGACATGGAGAAAGGTGACTGTTGGAGGTTCTGTTGAACCCTCACGCTGTAACTTTAGTGCTTGTGCAGTTGGAAATCGTGTTGTCCTTTTTGGTGGTGAAGGGGTTAATATGCAACCGATGAATGACACCTTCATATTGGATCTAAATGCCAGTAACCCAGAGTGGCAGCATGTCCAAGTCAGCTCTCCTCCACCTGGTCGTTGGGGCCATACACTATCTTGTGTGAATGGGTCCCATTTGGTGGTATTTGGAGGCTGTGGAAGGCAGGGCTTGCTAAATGATGTGTTCGTACTGGATTTGGACGCAAAGCCTCCAACTTGGCGTGAAATATCTGGGTTGGCTCCTCCGCTTCCTAGATCATGGCACAGTTCCTGCACTCTTGATGGCACCAAGTTGATAGTTTCAGGTGGCTGTGCAGATTCTGGAGTACTCCTAAGTGATACTTTTTTGCTTGATCTCTCAATGGAGAAACCTGTGTGGAGAGAGATACCAGTAGCATGGAAGCCCCCTTCAAGATTGGGCCACACCCTGTCTGTATATGGTGGTAGAAAAATTTTGATGTTTGGGGGCCTGGCCAAAAGTGGTCCACTTCGTTTTCGTTCCAGTGATGTGTTTACAATGGATCTGAGTGAGGATGAACCGTGTTGGAGGTGTGTTACAGGGAGTGGAATGCCCGGTTCTGGAAATCCAGGGGGCATGGCTCCTCCTCCTAGACTGGATCGATGTAGCTGTGAGCCTTCCAGGTGGGAGAATACTGATCTTTGGAGGGTCTGTTGCTGGTCTTCACTCTGCGTCGCAACTTTATCTCTTAGATCCAACGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
F-box-like Kelch_2 Kelch_4 PAS_9

Protein Analysis

565

Amino Acids

62.03

Weight (kDa)

5.23

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 40 - 156 1.7e-07 PAS fold
PAS_9 PF13426 47 - 157 6.5e-16 PAS domain
PAS_3 PF08447 62 - 156 8.3e-07 PAS fold
F-box-like PF12937 217 - 255 3.8e-09 F-box-like
Kelch_KLHDC2_KLHL20_DRC7 PF24681 286 - 480 1.6e-37 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 287 - 429 1.3e-10 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 289 - 392 4.5e-18 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 304 - 502 4.5e-09 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_HCF PF13854 343 - 483 3.1e-09 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 344 - 445 6.1e-20 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 405 - 514 1.5e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 4 cut(s) 136, 222, 676, 1256
AccB7I CCANNNNNTGG 2 cut(s) 431, 1111
AccBSI CCGCTC 1 cut(s) 598
AccI GTMKAC 1 cut(s) 306
AciI CCGC 4 cut(s) 101, 415, 598, 1217
AclWI GGATC 5 cut(s) 400, 1017, 1500, 1596, 1677
AcoI YGGCCR 1 cut(s) 1443
AcsI RAATTY 4 cut(s) 121, 356, 536, 1422
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 4 cut(s) 224, 648, 1161, 1299
AfiI CCNNNNNNNGG 7 cut(s) 232, 431, 509, 591, 1064, 1111, 1550
AgsI TTSAA 6 cut(s) 209, 356, 519, 863, 905, 1383
AhlI ACTAGT 1 cut(s) 301
AjiI CACGTC 1 cut(s) 457
AjnI CCWGG 6 cut(s) 588, 808, 1063, 1440, 1558, 1610
AluBI AGCT 6 cut(s) 30, 60, 727, 850, 1053, 1599
AluI AGCT 6 cut(s) 30, 60, 727, 850, 1053, 1599
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 220, 678
AlwI GGATC 5 cut(s) 400, 1017, 1500, 1596, 1677
AlwNI CAGNNNCTG 2 cut(s) 1242, 1292
Ama87I CYCGRG 3 cut(s) 94, 217, 789
AoxI GGCC 6 cut(s) 275, 801, 1075, 1390, 1438, 1443
ApeKI GCWGC 3 cut(s) 581, 866, 1036
ApoI RAATTY 4 cut(s) 121, 356, 536, 1422
Asp700I GAANNNNTTC 1 cut(s) 125
Asp718I GGTACC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 8 cut(s) 98, 275, 500, 1075, 1101, 1390, 1438, 1454
AsuC2I CCSGG 4 cut(s) 95, 96, 510, 1545
AsuHPI GGTGA 2 cut(s) 895, 977
AsuII TTCGAA 1 cut(s) 731
AvaI CYCGRG 3 cut(s) 94, 217, 789
AvaII GGWCC 4 cut(s) 98, 500, 1101, 1454
BaeGI GKGCMC 1 cut(s) 510
BalI TGGCCA 1 cut(s) 1445
BanI GGYRCC 4 cut(s) 136, 222, 676, 1256
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 2 cut(s) 747, 1644
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 3 cut(s) 568, 878, 1048
BccI CCATC 4 cut(s) 47, 191, 383, 1247
BceAI ACGGC 3 cut(s) 124, 150, 788
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 6 cut(s) 590, 810, 1065, 1442, 1560, 1612
BcnI CCSGG 4 cut(s) 95, 96, 510, 1545
BcoDI GTCTC 2 cut(s) 220, 678
BcuI ACTAGT 1 cut(s) 301
BfaI CTAG 6 cut(s) 302, 497, 681, 824, 1224, 1581
BfmI CTRYAG 2 cut(s) 312, 709
BfoI RGCGCY 1 cut(s) 140
BisI GCNGC 4 cut(s) 416, 582, 867, 1037
BlsI GCNGC 4 cut(s) 417, 583, 868, 1038
BmcAI AGTACT 1 cut(s) 1299
Bme18I GGWCC 4 cut(s) 98, 500, 1101, 1454
BmeT110I CYCGRG 3 cut(s) 94, 217, 789
BmgBI CACGTC 1 cut(s) 457
BmgT120I GGNCC 8 cut(s) 98, 275, 500, 1075, 1101, 1390, 1438, 1454
BmsI GCATC 2 cut(s) 304, 701
BpiI GAAGAC 2 cut(s) 747, 1644
BpmI CTGGAG 2 cut(s) 362, 1314
Bpu14I TTCGAA 1 cut(s) 731
BpuEI CTTGAG 1 cut(s) 641
BpuMI CCSGG 4 cut(s) 95, 96, 510, 1545
Bsa29I ATCGAT 1 cut(s) 1591
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 7 cut(s) 33, 93, 94, 231, 361, 589, 1559
BsaXI ACNNNNNCTCC 4 cut(s) 885, 915, 1627, 1657
Bsc4I CCNNNNNNNGG 7 cut(s) 232, 431, 509, 591, 1064, 1111, 1550
Bse118I RCCGGY 1 cut(s) 255
Bse1I ACTGG 5 cut(s) 1020, 1167, 1361, 1473, 1590
Bse3DI GCAATG 1 cut(s) 687
BseBI CCWGG 6 cut(s) 590, 810, 1065, 1442, 1560, 1612
BseCI ATCGAT 1 cut(s) 1591
BseDI CCNNGG 7 cut(s) 33, 93, 94, 231, 361, 589, 1559
BseGI GGATG 4 cut(s) 58, 295, 442, 1510
BseLI CCNNNNNNNGG 7 cut(s) 232, 431, 509, 591, 1064, 1111, 1550
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 2 cut(s) 17, 1487
BseNI ACTGG 5 cut(s) 1020, 1167, 1361, 1473, 1590
BseRI GAGGAG 9 cut(s) 50, 53, 56, 248, 359, 1047, 1203, 1563, 1566
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 3 cut(s) 568, 878, 1048
BsgI GTGCAG 3 cut(s) 954, 1228, 1305
BshFI GGCC 6 cut(s) 277, 803, 1077, 1392, 1440, 1445
BshNI GGYRCC 4 cut(s) 136, 222, 676, 1256
BshVI ATCGAT 1 cut(s) 1591
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 3 cut(s) 94, 217, 789
BsiSI CCGG 4 cut(s) 95, 256, 510, 1545
BslFI GGGAC 1 cut(s) 1087
BslI CCNNNNNNNGG 7 cut(s) 232, 431, 509, 591, 1064, 1111, 1550
BsmAI GTCTC 2 cut(s) 220, 678
BsmFI GGGAC 1 cut(s) 1087
BsmI GAATGC 1 cut(s) 1545
BsnI GGCC 6 cut(s) 277, 803, 1077, 1392, 1440, 1445
Bso31I GGTCTC 1 cut(s) 220
BsoBI CYCGRG 3 cut(s) 94, 217, 789
Bsp119I TTCGAA 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 32, 510
BspACI CCGC 4 cut(s) 101, 415, 598, 1217
BspANI GGCC 6 cut(s) 277, 803, 1077, 1392, 1440, 1445
BspCNI CTCAG 2 cut(s) 18, 1488
BspDI ATCGAT 1 cut(s) 1591
BspMAI CTGCAG 1 cut(s) 316
BspPI GGATC 5 cut(s) 400, 1017, 1500, 1596, 1677
BspT104I TTCGAA 1 cut(s) 731
BspT107I GGYRCC 4 cut(s) 136, 222, 676, 1256
BspTNI GGTCTC 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 598
BsrDI GCAATG 1 cut(s) 687
BsrFI RCCGGY 1 cut(s) 255
BsrI ACTGG 5 cut(s) 1020, 1167, 1361, 1473, 1590
BssAI RCCGGY 1 cut(s) 255
BssECI CCNNGG 7 cut(s) 33, 93, 94, 231, 361, 589, 1559
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 1 cut(s) 361
Bst2UI CCWGG 6 cut(s) 590, 810, 1065, 1442, 1560, 1612
Bst4CI ACNGT 6 cut(s) 202, 227, 720, 889, 1238, 1512
Bst6I CTCTTC 1 cut(s) 812
BstACI GRCGYC 1 cut(s) 137
BstAPI GCANNNNNTGC 2 cut(s) 320, 1141
BstBI TTCGAA 1 cut(s) 731
BstC8I GCNNGC 1 cut(s) 1142
BstDEI CTNAG 4 cut(s) 26, 1304, 1496, 1679
BstF5I GGATG 4 cut(s) 58, 295, 442, 1510
BstH2I RGCGCY 1 cut(s) 140
BstHHI GCGC 1 cut(s) 139
BstMAI GTCTC 2 cut(s) 220, 678
BstMWI GCNNNNNNNGC 2 cut(s) 320, 1141
BstNI CCWGG 6 cut(s) 590, 810, 1065, 1442, 1560, 1612
BstNSI RCATGY 1 cut(s) 1043
BstSFI CTRYAG 2 cut(s) 312, 709
BstSLI GKGCMC 1 cut(s) 510
BstV1I GCAGC 3 cut(s) 568, 878, 1048
BstV2I GAAGAC 2 cut(s) 747, 1644
BstX2I RGATCY 4 cut(s) 392, 1009, 1492, 1682
BstXI CCANNNNNNTGG 1 cut(s) 1368
BstYI RGATCY 4 cut(s) 392, 1009, 1492, 1682
Bsu15I ATCGAT 1 cut(s) 1591
BsuRI GGCC 6 cut(s) 277, 803, 1077, 1392, 1440, 1445
BsuTUI ATCGAT 1 cut(s) 1591
BtrI CACGTC 1 cut(s) 457
BtsCI GGATG 4 cut(s) 58, 295, 442, 1510
BtsIMutI CAGTG 2 cut(s) 198, 1480
Cac8I GCNNGC 1 cut(s) 1142
CaiI CAGNNNCTG 2 cut(s) 1242, 1292
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 255
Cfr13I GGNCC 8 cut(s) 98, 275, 500, 1075, 1101, 1390, 1438, 1454
Cfr9I CCCGGG 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 1591
CseI GACGC 3 cut(s) 170, 1181, 1651
CsiI ACCWGGT 1 cut(s) 1063
Csp6I GTAC 4 cut(s) 223, 647, 1160, 1298
CviAII CATG 6 cut(s) 771, 873, 1040, 1230, 1368, 1567
CviQI GTAC 4 cut(s) 223, 647, 1160, 1298
DdeI CTNAG 4 cut(s) 26, 1304, 1496, 1679
DinI GGCGCC 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 1443
Eam1104I CTCTTC 1 cut(s) 812
EarI CTCTTC 1 cut(s) 812
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 220
Eco47I GGWCC 4 cut(s) 98, 500, 1101, 1454
Eco53kI GAGCTC 1 cut(s) 30
Eco88I CYCGRG 3 cut(s) 94, 217, 789
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 3 cut(s) 500, 1101, 1438
EcoRI GAATTC 1 cut(s) 356
EcoRII CCWGG 6 cut(s) 588, 808, 1063, 1440, 1558, 1610
EcoT14I CCWWGG 1 cut(s) 361
EcoT22I ATGCAT 1 cut(s) 772
EcoT38I GRGCYC 1 cut(s) 32
EgeI GGCGCC 1 cut(s) 138
EheI GGCGCC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 361
FaeI CATG 6 cut(s) 774, 876, 1043, 1233, 1371, 1570
FalI AAGNNNNNCTT 2 cut(s) 1297, 1329
FaqI GGGAC 1 cut(s) 1087
FatI CATG 6 cut(s) 770, 872, 1039, 1229, 1367, 1566
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 4 cut(s) 416, 582, 867, 1037
FokI GGATG 4 cut(s) 65, 282, 449, 1517
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 4 cut(s) 416, 582, 867, 1037
FspBI CTAG 6 cut(s) 302, 497, 681, 824, 1224, 1581
GlaI GCGC 1 cut(s) 138
GluI GCNGC 4 cut(s) 416, 582, 867, 1037
GsuI CTGGAG 2 cut(s) 362, 1314
HaeII RGCGCY 1 cut(s) 140
HaeIII GGCC 6 cut(s) 277, 803, 1077, 1392, 1440, 1445
HapII CCGG 4 cut(s) 95, 256, 510, 1545
HgaI GACGC 3 cut(s) 170, 1181, 1651
HhaI GCGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 6 cut(s) 774, 876, 1043, 1233, 1371, 1570
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 5 cut(s) 308, 466, 527, 661, 1289
HpaII CCGG 4 cut(s) 95, 256, 510, 1545
HphI GGTGA 2 cut(s) 895, 977
Hpy166II GTNNAC 4 cut(s) 196, 307, 1457, 1485
Hpy188I TCNGA 5 cut(s) 22, 27, 34, 544, 1497
Hpy8I GTNNAC 4 cut(s) 196, 307, 1457, 1485
Hpy99I CGWCG 5 cut(s) 80, 83, 86, 119, 1667
HpyAV CCTTC 7 cut(s) 285, 595, 962, 1009, 1125, 1389, 1617
HpyCH4III ACNGT 6 cut(s) 202, 227, 720, 889, 1238, 1512
HpyCH4IV ACGT 3 cut(s) 84, 192, 456
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 1141
HpyF3I CTNAG 4 cut(s) 26, 1304, 1496, 1679
HpySE526I ACGT 3 cut(s) 84, 192, 456
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 6 cut(s) 774, 876, 1043, 1233, 1371, 1570
HspAI GCGC 1 cut(s) 137
KasI GGCGCC 1 cut(s) 136
KflI GGGWCCC 1 cut(s) 1101
KpnI GGTACC 1 cut(s) 226
LmnI GCTCC 6 cut(s) 35, 57, 595, 847, 1216, 1576
Lsp1109I GCAGC 3 cut(s) 568, 878, 1048
LweI GCATC 2 cut(s) 304, 701
MabI ACCWGGT 1 cut(s) 1063
MaeI CTAG 6 cut(s) 302, 497, 681, 824, 1224, 1581
MaeII ACGT 3 cut(s) 84, 192, 456
MaeIII GTNAC 5 cut(s) 154, 883, 917, 1022, 1525
MbiI CCGCTC 1 cut(s) 598
MboII GAAGA 5 cut(s) 466, 745, 752, 829, 1644
MfeI CAATTG 2 cut(s) 250, 632
MflI RGATCY 4 cut(s) 392, 1009, 1492, 1682
MhlI GDGCHC 2 cut(s) 32, 510
MlsI TGGCCA 1 cut(s) 1445
MluCI AATT 9 cut(s) 16, 121, 250, 327, 356, 536, 632, 671, 1422
MluNI TGGCCA 1 cut(s) 1445
Mly113I GGCGCC 1 cut(s) 137
MlyI GAGTC 2 cut(s) 302, 536
MmeI TCCRAC 4 cut(s) 871, 919, 1208, 1496
Mox20I TGGCCA 1 cut(s) 1445
Mph1103I ATGCAT 1 cut(s) 772
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 1445
MseI TTAA 3 cut(s) 330, 374, 975
Msp20I TGGCCA 1 cut(s) 1445
MspI CCGG 4 cut(s) 95, 256, 510, 1545
MunI CAATTG 2 cut(s) 250, 632
Mva1269I GAATGC 1 cut(s) 1545
MvaI CCWGG 6 cut(s) 590, 810, 1065, 1442, 1560, 1612
MwoI GCNNNNNNNGC 2 cut(s) 320, 1141
NarI GGCGCC 1 cut(s) 137
NciI CCSGG 4 cut(s) 95, 96, 510, 1545
NlaIII CATG 6 cut(s) 774, 876, 1043, 1233, 1371, 1570
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 154, 883
NsiI ATGCAT 1 cut(s) 772
NspI RCATGY 1 cut(s) 1043
NspV TTCGAA 1 cut(s) 731
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 1545
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 3 cut(s) 466, 661, 1289
PflMI CCANNNNNTGG 2 cut(s) 431, 1111
PkrI GCNGC 4 cut(s) 417, 583, 868, 1038
PleI GAGTC 2 cut(s) 302, 535
PluTI GGCGCC 1 cut(s) 140
PpsI GAGTC 2 cut(s) 302, 535
PpuMI RGGWCCY 2 cut(s) 500, 1101
Psp124BI GAGCTC 1 cut(s) 32
Psp5II RGGWCCY 2 cut(s) 500, 1101
Psp6I CCWGG 6 cut(s) 588, 808, 1063, 1440, 1558, 1610
PspGI CCWGG 6 cut(s) 588, 808, 1063, 1440, 1558, 1610
PspPI GGNCC 8 cut(s) 98, 275, 500, 1075, 1101, 1390, 1438, 1454
PspPPI RGGWCCY 2 cut(s) 500, 1101
PstI CTGCAG 1 cut(s) 316
PstNI CAGNNNCTG 2 cut(s) 1242, 1292
PsuI RGATCY 4 cut(s) 392, 1009, 1492, 1682
RsaI GTAC 4 cut(s) 224, 648, 1161, 1299
RsaNI GTAC 4 cut(s) 223, 647, 1160, 1298
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 3 cut(s) 330, 374, 975
SatI GCNGC 4 cut(s) 416, 582, 867, 1037
Sau96I GGNCC 8 cut(s) 98, 275, 500, 1075, 1101, 1390, 1438, 1454
ScaI AGTACT 1 cut(s) 1299
SchI GAGTC 2 cut(s) 302, 536
SduI GDGCHC 2 cut(s) 32, 510
SexAI ACCWGGT 1 cut(s) 1063
SfaNI GCATC 2 cut(s) 304, 701
SfcI CTRYAG 2 cut(s) 312, 709
SfoI GGCGCC 1 cut(s) 138
SfuI TTCGAA 1 cut(s) 731
SinI GGWCC 4 cut(s) 98, 500, 1101, 1454
SmaI CCCGGG 1 cut(s) 96
SmlI CTYRAG 1 cut(s) 656
SmoI CTYRAG 1 cut(s) 656
SpeI ACTAGT 1 cut(s) 301
Sse9I AATT 9 cut(s) 16, 121, 250, 327, 356, 536, 632, 671, 1422
SsiI CCGC 4 cut(s) 101, 415, 598, 1217
SspDI GGCGCC 1 cut(s) 136
SspMI CTAG 6 cut(s) 302, 497, 681, 824, 1224, 1581
SstI GAGCTC 1 cut(s) 32
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 6 cut(s) 202, 227, 720, 889, 1238, 1512
TaiI ACGT 3 cut(s) 87, 195, 459
TaqI TCGA 5 cut(s) 117, 168, 668, 731, 1591
TasI AATT 9 cut(s) 16, 121, 250, 327, 356, 536, 632, 671, 1422
TatI WGTACW 1 cut(s) 1297
TauI GCSGC 1 cut(s) 418
TfiI GAWTC 3 cut(s) 466, 661, 1289
Tru1I TTAA 3 cut(s) 330, 374, 975
Tru9I TTAA 3 cut(s) 330, 374, 975
TscAI CASTG 2 cut(s) 205, 1480
TseFI GTSAC 2 cut(s) 154, 883
TseI GCWGC 3 cut(s) 581, 866, 1036
Tsp45I GTSAC 2 cut(s) 154, 883
TspDTI ATGAA 5 cut(s) 419, 753, 991, 1004, 1521
TspGWI ACGGA 4 cut(s) 96, 173, 494, 563
TspMI CCCGGG 1 cut(s) 94
TspRI CASTG 2 cut(s) 205, 1480
Van91I CCANNNNNTGG 2 cut(s) 431, 1111
VpaK11BI GGWCC 4 cut(s) 98, 500, 1101, 1454
XapI RAATTY 4 cut(s) 121, 356, 536, 1422
XceI RCATGY 1 cut(s) 1043
XcmI CCANNNNNNNNNTGG 1 cut(s) 1068
XmaI CCCGGG 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 6 cut(s) 302, 497, 681, 824, 1224, 1581
ZrmI AGTACT 1 cut(s) 1299
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.