Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0010333

Physical Location & Seq

Genomic Coordinates Reverse (-)

28302180 .. 28306914

Gene Structure 4735 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1256500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGGCGAATCTTGTCCCTGGCGTTCTCCTCAAGCTGCTACAGCATATGAACACGGATGTCAAAGTCGCCGGCGAGCACAGGTCGTCTCTGTTGCAAGTGGTGAGCATTGTTCCGGCGCTTGCAGGCGGTGAGCTTTTCCCAAACCAAGGTTTCTATCTTAAGGTATCAGATTCCTCTCATGCCACCTATGTATCTCTCCCTGATGAACATGATGATTTAATTCTCAGTGATAAGATTCAACTGGGTCAGTTTATTCATGTCGACCGGCTTCAGGCTGCCACCCCGGTCCCCATTCTCCAGGGGGTTAGGCCTGTGCCAGGGAGGCACTCTTGCGTAGGGAGCCCTGAAGACATCGTTGCAACTCATTCTCTTGGTTTTCTGAACAATAATTCGTCTTCGGGTTCGAAAACTTTAGAGAAAGTACAGTCTGAGTATAAGGTATTAGGAAATAGTCATGCCGGAGAAAGGGATAAACATGGACTAGTGAGGTCGAATAGTAGTGCTAAGGCGGATAAATTGGATAAGAAAATAGGGTCTTTTGACAGATCAAAGTCTCAGCCATCAAAACCTGCATTAACAATAGATATGAAGAGGGAACCTTTGCCGAGAATGAAGTCATTGAATTCACGGTCTATCCCGTCATCTCCATCAAGTTGTTATTCTTTGCCGACTTCTTTTGAGAAATTTGCAAATGGGATCAAGCATCATGCAAAAGTTAAGGGAACACCAAAGGTGGGAGTGGTGGAAAGGGCAAGTTCTGTACATGGGGCGAGTCCCGGGAGGAGATCAGTGGTGGGAAACCCAATAAAGAATTTTGTTCAGGGGTTTGAGCTGGAGGCCAAGGCCCTGAGGAAGAGCTGGGAAGGAAATATGGAGGGGAAGTCTAAAGAGATGTCTGCTCCTAGAAAAAGTATCTCAACTGAAAGGTTGCCATATAAAGAGGACAATAGGACCCAGATATCTGCAAAGTCATTGAAAGAGGAGAGTAAAGTTCAAACGTCAACAAAGAAATTTACAGCAAATGGAACTTTGAGTGATCTAGACAGGTCAAGTAAACTTAGAGTATCTGTTGGAAAGAAATCTTCAGATGTAGCCAGTAATGGATTTCCTGGAAACTTGTTCAGGGTTTCCTTAAATAACAGAAAATTGACTGATGGAGTTCCATGGGCTTCGCTCCCATTGTCAGTTGCAAAGCTTGGTAAGGAAGTTATGAGGCACAGGGATGCTGCACAAATGGCAGCAAAAGAGGCGATGCAGGAGGCTTCTGCTGCAGAGAACTTACTACGATGTTTGAGCATGTATTCAGAGCTAAGTACCTCTGCCAAGGAAGACAACCCTCAGCCTGCAGTAGAGCAGTTTCTTGCCCTCCATGCTAGTTTGAGTAATGCTCGCGTAGTTGCTGATTCTCTATCCAAAATCATGCCAGCTGGTTCTTCCCCAGACCGTGAAGAAACCCCCCCATCAGAAGTACTGAAGGTAGCTTCAGACAGACGTAAACAGGCAGCATCTTGGGTACAAGCTGCATTAGCCACCAATCTGTCATCTTTTGCTGTATTTAATAAAGAGTCCAGTTTAATCTCTGTTCAAGCTTCAGCTTCCTCTCAAAACCAAAAGATTGTCTCTGCAAATCAACCCGTGTTAGTTCTAGAAAATTCTACAAAGAATGCTTCAACAAAATCCCAAGGCAAACTTCGCCAGACGGTGGGTTCCAAGCTTGGGACACCAGGGACCCCTCGCCGACTAGGGGATGGATCAGTCATCAATCAAAAGCCGCAGGCACGACCTCTGCCTGAATGGAATAAAGGAAATGGCCTGGACGAGGTTGTTGACTTGGCTGAAAAGTTGCGACTGCAGTCACAAGCTTGGTTTTTGGGATTTGTGGAGAGGTTCCTAGATGCTGACGTTGACAGTTCAGCTCTTTCAGATAATGGACAAATAGCAGGAATGCTAACTCAGCTCAAGAGTGTGAATGATTGGTTGGATAACATTGGTTCTATTCAAGAAGGGGTTGAAACAACCGATGTTTCAGCGGAGACCATTGACAGCCTGAGGAAGAAGATCTATGAGTATCTTCTCACACATGTTGAATCTGCTGCCGCTGCACTTGGCGGTGGATCACAGTCATCACCTCGAATCCAAACACTAGATACAAAACTTAGAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF936

Protein Analysis

722

Amino Acids

77.86

Weight (kDa)

9.53

Isoelectric Point (pI)

40.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF936	PF06075	4 - 120	5.5e-58	Plant protein of unknown function (DUF936)
DUF6857	PF21647	378 - 702	1.6e-103	Domain of unknown function (DUF6857)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08760
fragaria_vesca	FvH4_1g23460
malus_domestica	MD04G1045400.v1.1 MD13G1256500.v1.1
prunus_persica	Prupe.1G198600_v2.0.a1
pyrus_communis	pycom04g03910 pycom13g27120
rosa_chinensis	RchiOBHm_Chr2g0118831
rosa_laevigata	RLG00000018424
rosa_multiflora	Rmu_sc0001814.1_g000010 Rmu_sc0015187.1_g000001 Rmu_sc0030646.1_g000003
rosa_roxburghii	Rroxscaffold_2G00124660 Rroxscaffold_2G00124670 Rroxscaffold_2G00125140
rosa_rugosa	Rorug02G0219100
rosa_samantha	Rh2AG276200 Rh2BG288000 Rh2DG283100 Rh2DG302200
rosa_wichuraiana	Rw2G021980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	577
AccB7I	CCANNNNNTGG	1 cut(s)	1702
AccI	GTMKAC	1 cut(s)	261
AccII	CGCG	1 cut(s)	1392
AciI	CCGC	6 cut(s)	126, 509, 1772, 2030, 2097, 2109
AclWI	GGATC	3 cut(s)	704, 1759, 2122
AcsI	RAATTY	5 cut(s)	622, 683, 811, 1010, 1651
AcuI	CTGAAG	6 cut(s)	254, 366, 1068, 1467, 1493, 1575
AfaI	GTAC	5 cut(s)	423, 762, 1315, 1470, 1515
AfiI	CCNNNNNNNGG	9 cut(s)	146, 271, 317, 465, 734, 1702, 1716, 1744, 1819
AflII	CTTAAG	1 cut(s)	158
AflIII	ACRYGT	1 cut(s)	2080
AgsI	TTSAA	9 cut(s)	239, 622, 976, 995, 1586, 1671, 2000, 2012, 2087
AhlI	ACTAGT	1 cut(s)	481
AjnI	CCWGG	6 cut(s)	16, 297, 316, 1108, 1723, 1812
AjuI	GAANNNNNNNTTGG	4 cut(s)	1674, 1706, 1961, 1993
Alw21I	GWGCWC	1 cut(s)	78
Alw26I	GTCTC	4 cut(s)	90, 558, 1624, 2027
AlwI	GGATC	3 cut(s)	704, 1759, 2122
Ama87I	CYCGRG	1 cut(s)	776
AoxI	GGCC	4 cut(s)	308, 837, 843, 1810
ApeKI	GCWGC	9 cut(s)	34, 275, 1226, 1238, 1268, 1502, 1520, 2093, 2099
ApoI	RAATTY	5 cut(s)	622, 683, 811, 1010, 1651
AspLEI	GCGC	1 cut(s)	118
AspS9I	GGNCC	4 cut(s)	286, 844, 951, 1728
AsuC2I	CCSGG	3 cut(s)	284, 777, 778
AsuHPI	GGTGA	3 cut(s)	112, 140, 2118
AsuII	TTCGAA	1 cut(s)	404
AvaI	CYCGRG	1 cut(s)	776
AvaII	GGWCC	3 cut(s)	286, 951, 1728
AxyI	CCTNAGG	2 cut(s)	848, 2048
BaeI	ACNNNNGTAYC	2 cut(s)	174, 207
BanII	GRGCYC	1 cut(s)	344
BbsI	GAAGAC	3 cut(s)	354, 387, 1335
Bbv12I	GWGCWC	1 cut(s)	78
BbvCI	CCTCAGC	1 cut(s)	1338
BbvI	GCAGC	9 cut(s)	21, 262, 1213, 1250, 1255, 1507, 1514, 2080, 2086
BccI	CCATC	5 cut(s)	568, 655, 1148, 1468, 1742
BciT130I	CCWGG	6 cut(s)	18, 299, 318, 1110, 1725, 1814
BcnI	CCSGG	3 cut(s)	284, 777, 778
BcoDI	GTCTC	4 cut(s)	90, 558, 1624, 2027
BcuI	ACTAGT	1 cut(s)	481
BfaI	CTAG	8 cut(s)	482, 903, 1040, 1374, 1646, 1742, 1892, 2144
BfmI	CTRYAG	4 cut(s)	38, 1269, 1344, 1850
BfoI	RGCGCY	1 cut(s)	119
BfrI	CTTAAG	1 cut(s)	158
BfuAI	ACCTGC	1 cut(s)	577
BglI	GCCNNNNNGGC	1 cut(s)	322
BglII	AGATCT	1 cut(s)	2058
BmcAI	AGTACT	1 cut(s)	1470
Bme1390I	CCNGG	9 cut(s)	18, 284, 299, 318, 777, 778, 1110, 1725, 1814
Bme18I	GGWCC	3 cut(s)	286, 951, 1728
BmeT110I	CYCGRG	1 cut(s)	776
BmgT120I	GGNCC	4 cut(s)	286, 844, 951, 1728
BmiI	GGNNCC	8 cut(s)	288, 341, 597, 953, 1708, 1729, 1730, 1889
BmrFI	CCNGG	9 cut(s)	18, 284, 299, 318, 777, 778, 1110, 1725, 1814
BmrI	ACTGGG	1 cut(s)	251
BmsI	GCATC	5 cut(s)	712, 1213, 1242, 1514, 1885
BmuI	ACTGGG	1 cut(s)	251
BoxI	GACNNNNGTC	1 cut(s)	1852
BpiI	GAAGAC	3 cut(s)	354, 387, 1335
BplI	GAGNNNNNCTC	2 cut(s)	1372, 1404
BpmI	CTGGAG	2 cut(s)	281, 854
Bpu10I	CCTNAGC	2 cut(s)	504, 1338
Bpu14I	TTCGAA	1 cut(s)	404
BpuEI	CTTGAG	2 cut(s)	14, 1943
BpuMI	CCSGG	3 cut(s)	284, 777, 778
BsaI	GGTCTC	1 cut(s)	2027
BsaXI	ACNNNNNCTCC	2 cut(s)	866, 896
Bsc4I	CCNNNNNNNGG	9 cut(s)	146, 271, 317, 465, 734, 1702, 1716, 1744, 1819
Bse118I	RCCGGY	2 cut(s)	68, 264
Bse1I	ACTGG	3 cut(s)	246, 1095, 1569
Bse21I	CCTNAGG	2 cut(s)	848, 2048
BseBI	CCWGG	6 cut(s)	18, 299, 318, 1110, 1725, 1814
BseGI	GGATG	3 cut(s)	61, 1228, 1753
BseLI	CCNNNNNNNGG	9 cut(s)	146, 271, 317, 465, 734, 1702, 1716, 1744, 1819
BseMII	CTCAG	7 cut(s)	238, 420, 569, 839, 1352, 1967, 2039
BseNI	ACTGG	3 cut(s)	246, 1095, 1569
BseRI	GAGGAG	3 cut(s)	17, 796, 995
BseXI	GCAGC	9 cut(s)	21, 262, 1213, 1250, 1255, 1507, 1514, 2080, 2086
BseYI	CCCAGC	1 cut(s)	858
BsgI	GTGCAG	2 cut(s)	1212, 2085
Bsh1236I	CGCG	1 cut(s)	1392
Bsh1285I	CGRYCG	1 cut(s)	265
BshFI	GGCC	4 cut(s)	310, 839, 845, 1812
BsiEI	CGRYCG	1 cut(s)	265
BsiHKAI	GWGCWC	1 cut(s)	78
BsiHKCI	CYCGRG	1 cut(s)	776
BsiSI	CCGG	6 cut(s)	69, 113, 265, 284, 459, 777
BslFI	GGGAC	4 cut(s)	272, 759, 1732, 1741
BslI	CCNNNNNNNGG	9 cut(s)	146, 271, 317, 465, 734, 1702, 1716, 1744, 1819
BsmAI	GTCTC	4 cut(s)	90, 558, 1624, 2027
BsmBI	CGTCTC	1 cut(s)	90
BsmFI	GGGAC	4 cut(s)	272, 759, 1732, 1741
BsmI	GAATGC	2 cut(s)	1669, 1950
BsnI	GGCC	4 cut(s)	310, 839, 845, 1812
Bso31I	GGTCTC	1 cut(s)	2027
BsoBI	CYCGRG	1 cut(s)	776
Bsp119I	TTCGAA	1 cut(s)	404
Bsp1286I	GDGCHC	2 cut(s)	78, 344
Bsp1407I	TGTACA	1 cut(s)	760
Bsp143I	GATC	7 cut(s)	545, 696, 785, 1036, 1751, 2058, 2114
Bsp19I	CCATGG	1 cut(s)	1163
BspACI	CCGC	6 cut(s)	126, 509, 1772, 2030, 2097, 2109
BspANI	GGCC	4 cut(s)	310, 839, 845, 1812
BspCNI	CTCAG	7 cut(s)	237, 421, 568, 840, 1351, 1966, 2040
BspFNI	CGCG	1 cut(s)	1392
BspLI	GGNNCC	8 cut(s)	288, 341, 597, 953, 1708, 1729, 1730, 1889
BspMAI	CTGCAG	3 cut(s)	1273, 1348, 1854
BspMI	ACCTGC	1 cut(s)	577
BspPI	GGATC	3 cut(s)	704, 1759, 2122
BspQI	GCTCTTC	1 cut(s)	848
BspT104I	TTCGAA	1 cut(s)	404
BspTI	CTTAAG	1 cut(s)	158
BspTNI	GGTCTC	1 cut(s)	2027
BsrFI	RCCGGY	2 cut(s)	68, 264
BsrGI	TGTACA	1 cut(s)	760
BsrI	ACTGG	3 cut(s)	246, 1095, 1569
BssAI	RCCGGY	2 cut(s)	68, 264
BssMI	GATC	7 cut(s)	545, 696, 785, 1036, 1751, 2058, 2114
BssT1I	CCWWGG	5 cut(s)	145, 840, 1163, 1323, 1681
Bst2UI	CCWGG	6 cut(s)	18, 299, 318, 1110, 1725, 1814
Bst4CI	ACNGT	6 cut(s)	426, 630, 1445, 1702, 1910, 2121
Bst6I	CTCTTC	2 cut(s)	584, 848
BstAFI	CTTAAG	1 cut(s)	158
BstAUI	TGTACA	1 cut(s)	760
BstBI	TTCGAA	1 cut(s)	404
BstC8I	GCNNGC	8 cut(s)	70, 74, 120, 124, 1344, 1390, 1425, 1776
BstDSI	CCRYGG	1 cut(s)	1163
BstENI	CCTNNNNNAGG	2 cut(s)	315, 1817
BstF5I	GGATG	3 cut(s)	61, 1228, 1753
BstFNI	CGCG	1 cut(s)	1392
BstH2I	RGCGCY	1 cut(s)	119
BstHHI	GCGC	1 cut(s)	118
BstKTI	GATC	7 cut(s)	548, 699, 788, 1039, 1754, 2061, 2117
BstMAI	GTCTC	4 cut(s)	90, 558, 1624, 2027
BstMBI	GATC	7 cut(s)	545, 696, 785, 1036, 1751, 2058, 2114
BstMCI	CGRYCG	1 cut(s)	265
BstNI	CCWGG	6 cut(s)	18, 299, 318, 1110, 1725, 1814
BstNSI	RCATGY	2 cut(s)	1300, 2084
BstPAI	GACNNNNGTC	1 cut(s)	1852
BstSCI	CCNGG	9 cut(s)	16, 282, 297, 316, 775, 776, 1108, 1723, 1812
BstSFI	CTRYAG	4 cut(s)	38, 1269, 1344, 1850
BstUI	CGCG	1 cut(s)	1392
BstV1I	GCAGC	9 cut(s)	21, 262, 1213, 1250, 1255, 1507, 1514, 2080, 2086
BstV2I	GAAGAC	3 cut(s)	354, 387, 1335
BstX2I	RGATCY	1 cut(s)	2058
BstYI	RGATCY	1 cut(s)	2058
Bsu36I	CCTNAGG	2 cut(s)	848, 2048
BsuRI	GGCC	4 cut(s)	310, 839, 845, 1812
BtgI	CCRYGG	1 cut(s)	1163
BtgZI	GCGATG	1 cut(s)	1265
BtsCI	GGATG	3 cut(s)	61, 1228, 1753
BtsIMutI	CAGTG	2 cut(s)	232, 795
BveI	ACCTGC	1 cut(s)	577
Cac8I	GCNNGC	8 cut(s)	70, 74, 120, 124, 1344, 1390, 1425, 1776
CfoI	GCGC	1 cut(s)	118
Cfr10I	RCCGGY	2 cut(s)	68, 264
Cfr13I	GGNCC	4 cut(s)	286, 844, 951, 1728
Cfr9I	CCCGGG	1 cut(s)	776
Csp6I	GTAC	5 cut(s)	422, 761, 1314, 1469, 1514
CviQI	GTAC	5 cut(s)	422, 761, 1314, 1469, 1514
DpnI	GATC	7 cut(s)	547, 698, 787, 1038, 1753, 2060, 2116
DpnII	GATC	7 cut(s)	545, 696, 785, 1036, 1751, 2058, 2114
Eam1104I	CTCTTC	2 cut(s)	584, 848
EarI	CTCTTC	2 cut(s)	584, 848
EciI	GGCGGA	1 cut(s)	524
Eco130I	CCWWGG	5 cut(s)	145, 840, 1163, 1323, 1681
Eco147I	AGGCCT	1 cut(s)	310
Eco24I	GRGCYC	1 cut(s)	344
Eco31I	GGTCTC	1 cut(s)	2027
Eco32I	GATATC	1 cut(s)	960
Eco47I	GGWCC	3 cut(s)	286, 951, 1728
Eco57I	CTGAAG	6 cut(s)	254, 366, 1068, 1467, 1493, 1575
Eco81I	CCTNAGG	2 cut(s)	848, 2048
Eco88I	CYCGRG	1 cut(s)	776
EcoNI	CCTNNNNNAGG	2 cut(s)	315, 1817
EcoO109I	RGGNCCY	3 cut(s)	844, 951, 1728
EcoRI	GAATTC	1 cut(s)	622
EcoRII	CCWGG	6 cut(s)	16, 297, 316, 1108, 1723, 1812
EcoRV	GATATC	1 cut(s)	960
EcoT14I	CCWWGG	5 cut(s)	145, 840, 1163, 1323, 1681
EcoT38I	GRGCYC	1 cut(s)	344
ErhI	CCWWGG	5 cut(s)	145, 840, 1163, 1323, 1681
Esp3I	CGTCTC	1 cut(s)	90
FalI	AAGNNNNNCTT	2 cut(s)	1674, 1706
FaqI	GGGAC	4 cut(s)	272, 759, 1732, 1741
FauNDI	CATATG	1 cut(s)	45
FblI	GTMKAC	1 cut(s)	261
FokI	GGATG	3 cut(s)	68, 1235, 1760
FriOI	GRGCYC	1 cut(s)	344
FspBI	CTAG	8 cut(s)	482, 903, 1040, 1374, 1646, 1742, 1892, 2144
GlaI	GCGC	1 cut(s)	117
GsaI	CCCAGC	1 cut(s)	862
GsuI	CTGGAG	2 cut(s)	281, 854
HaeII	RGCGCY	1 cut(s)	119
HaeIII	GGCC	4 cut(s)	310, 839, 845, 1812
HapII	CCGG	6 cut(s)	69, 113, 265, 284, 459, 777
HhaI	GCGC	1 cut(s)	118
Hin6I	GCGC	1 cut(s)	116
HinP1I	GCGC	1 cut(s)	116
HincII	GTYRAC	4 cut(s)	262, 1002, 1828, 1906
HindII	GTYRAC	4 cut(s)	262, 1002, 1828, 1906
HindIII	AAGCTT	4 cut(s)	1193, 1587, 1712, 1860
HinfI	GANTC	8 cut(s)	7, 170, 235, 772, 1403, 1565, 2087, 2133
HpaII	CCGG	6 cut(s)	69, 113, 265, 284, 459, 777
HphI	GGTGA	3 cut(s)	112, 140, 2118
Hpy166II	GTNNAC	6 cut(s)	262, 1002, 1055, 1496, 1828, 1906
Hpy188I	TCNGA	8 cut(s)	169, 381, 430, 1087, 1306, 1465, 1486, 1924
Hpy188III	TCNNGA	4 cut(s)	1040, 1646, 1960, 2000
Hpy8I	GTNNAC	6 cut(s)	262, 1002, 1055, 1496, 1828, 1906
HpyAV	CCTTC	3 cut(s)	857, 1468, 1997
HpyCH4III	ACNGT	6 cut(s)	426, 630, 1445, 1702, 1910, 2121
HpyCH4IV	ACGT	3 cut(s)	998, 1492, 1902
HpySE526I	ACGT	3 cut(s)	998, 1492, 1902
HspAI	GCGC	1 cut(s)	116
KflI	GGGWCCC	1 cut(s)	1728
KroI	GCCGGC	1 cut(s)	68
KroNI	GCCGGC	1 cut(s)	70
Kzo9I	GATC	7 cut(s)	545, 696, 785, 1036, 1751, 2058, 2114
LguI	GCTCTTC	1 cut(s)	848
LmnI	GCTCC	3 cut(s)	339, 904, 1179
Lsp1109I	GCAGC	9 cut(s)	21, 262, 1213, 1250, 1255, 1507, 1514, 2080, 2086
LweI	GCATC	5 cut(s)	712, 1213, 1242, 1514, 1885
MaeI	CTAG	8 cut(s)	482, 903, 1040, 1374, 1646, 1742, 1892, 2144
MaeII	ACGT	3 cut(s)	998, 1492, 1902
MaeIII	GTNAC	1 cut(s)	1854
MalI	GATC	7 cut(s)	547, 698, 787, 1038, 1753, 2060, 2116
MboI	GATC	7 cut(s)	545, 696, 785, 1036, 1751, 2058, 2114
MflI	RGATCY	1 cut(s)	2058
MhlI	GDGCHC	2 cut(s)	78, 344
MluCI	AATT	9 cut(s)	219, 388, 515, 622, 683, 811, 1010, 1145, 1651
MlyI	GAGTC	2 cut(s)	781, 1574
MmeI	TCCRAC	2 cut(s)	1051, 1959
MreI	CGCCGGCG	1 cut(s)	68
MroNI	GCCGGC	1 cut(s)	68
MseI	TTAA	7 cut(s)	159, 218, 575, 717, 1133, 1557, 1574
MslI	CAYNNNNRTG	1 cut(s)	1221
MspA1I	CMGCKG	3 cut(s)	1427, 2030, 2099
MspCI	CTTAAG	1 cut(s)	158
MspI	CCGG	6 cut(s)	69, 113, 265, 284, 459, 777
MspR9I	CCNGG	9 cut(s)	18, 284, 299, 318, 777, 778, 1110, 1725, 1814
Mva1269I	GAATGC	2 cut(s)	1669, 1950
MvaI	CCWGG	6 cut(s)	18, 299, 318, 1110, 1725, 1814
MvnI	CGCG	1 cut(s)	1392
NaeI	GCCGGC	1 cut(s)	70
NciI	CCSGG	3 cut(s)	284, 777, 778
NcoI	CCATGG	1 cut(s)	1163
NdeI	CATATG	1 cut(s)	45
NdeII	GATC	7 cut(s)	545, 696, 785, 1036, 1751, 2058, 2114
NgoMIV	GCCGGC	1 cut(s)	68
NlaIV	GGNNCC	8 cut(s)	288, 341, 597, 953, 1708, 1729, 1730, 1889
NmeAIII	GCCGAG	1 cut(s)	630
NmuCI	GTSAC	1 cut(s)	1854
NspI	RCATGY	2 cut(s)	1300, 2084
NspV	TTCGAA	1 cut(s)	404
PceI	AGGCCT	1 cut(s)	310
PciI	ACATGT	1 cut(s)	2080
PciSI	GCTCTTC	1 cut(s)	848
PctI	GAATGC	2 cut(s)	1669, 1950
PdiI	GCCGGC	1 cut(s)	70
PfeI	GAWTC	6 cut(s)	7, 170, 235, 1403, 2087, 2133
PflMI	CCANNNNNTGG	1 cut(s)	1702
PfoI	TCCNGGA	1 cut(s)	1108
PleI	GAGTC	2 cut(s)	780, 1573
PpsI	GAGTC	2 cut(s)	780, 1573
PpuMI	RGGWCCY	2 cut(s)	951, 1728
PscI	ACATGT	1 cut(s)	2080
PshAI	GACNNNNGTC	1 cut(s)	1852
Psp5II	RGGWCCY	2 cut(s)	951, 1728
Psp6I	CCWGG	6 cut(s)	16, 297, 316, 1108, 1723, 1812
PspFI	CCCAGC	1 cut(s)	858
PspGI	CCWGG	6 cut(s)	16, 297, 316, 1108, 1723, 1812
PspN4I	GGNNCC	8 cut(s)	288, 341, 597, 953, 1708, 1729, 1730, 1889
PspPI	GGNCC	4 cut(s)	286, 844, 951, 1728
PspPPI	RGGWCCY	2 cut(s)	951, 1728
PstI	CTGCAG	3 cut(s)	1273, 1348, 1854
PsuI	RGATCY	1 cut(s)	2058
PvuII	CAGCTG	1 cut(s)	1427
RsaI	GTAC	5 cut(s)	423, 762, 1315, 1470, 1515
RsaNI	GTAC	5 cut(s)	422, 761, 1314, 1469, 1514
RseI	CAYNNNNRTG	1 cut(s)	1221
SalI	GTCGAC	1 cut(s)	260
SapI	GCTCTTC	1 cut(s)	848
SaqAI	TTAA	7 cut(s)	159, 218, 575, 717, 1133, 1557, 1574
Sau3AI	GATC	7 cut(s)	545, 696, 785, 1036, 1751, 2058, 2114
Sau96I	GGNCC	4 cut(s)	286, 844, 951, 1728
ScaI	AGTACT	1 cut(s)	1470
SchI	GAGTC	2 cut(s)	781, 1574
ScrFI	CCNGG	9 cut(s)	18, 284, 299, 318, 777, 778, 1110, 1725, 1814
SduI	GDGCHC	2 cut(s)	78, 344
SfaNI	GCATC	5 cut(s)	712, 1213, 1242, 1514, 1885
SfcI	CTRYAG	4 cut(s)	38, 1269, 1344, 1850
SfuI	TTCGAA	1 cut(s)	404
SgrAI	CRCCGGYG	1 cut(s)	68
SinI	GGWCC	3 cut(s)	286, 951, 1728
SmaI	CCCGGG	1 cut(s)	778
SmiMI	CAYNNNNRTG	1 cut(s)	1221
SmlI	CTYRAG	3 cut(s)	29, 158, 1958
SmoI	CTYRAG	3 cut(s)	29, 158, 1958
SpeI	ACTAGT	1 cut(s)	481
Sse9I	AATT	9 cut(s)	219, 388, 515, 622, 683, 811, 1010, 1145, 1651
SseBI	AGGCCT	1 cut(s)	310
SsiI	CCGC	6 cut(s)	126, 509, 1772, 2030, 2097, 2109
SspMI	CTAG	8 cut(s)	482, 903, 1040, 1374, 1646, 1742, 1892, 2144
StuI	AGGCCT	1 cut(s)	310
StyD4I	CCNGG	9 cut(s)	16, 282, 297, 316, 775, 776, 1108, 1723, 1812
StyI	CCWWGG	5 cut(s)	145, 840, 1163, 1323, 1681
TaaI	ACNGT	6 cut(s)	426, 630, 1445, 1702, 1910, 2121
TaiI	ACGT	3 cut(s)	1001, 1495, 1905
TaqI	TCGA	4 cut(s)	261, 404, 491, 2131
TasI	AATT	9 cut(s)	219, 388, 515, 622, 683, 811, 1010, 1145, 1651
TatI	WGTACW	3 cut(s)	421, 760, 1468
TauI	GCSGC	2 cut(s)	1774, 2099
TfiI	GAWTC	6 cut(s)	7, 170, 235, 1403, 2087, 2133
Tru1I	TTAA	7 cut(s)	159, 218, 575, 717, 1133, 1557, 1574
Tru9I	TTAA	7 cut(s)	159, 218, 575, 717, 1133, 1557, 1574
TscAI	CASTG	2 cut(s)	232, 795
TseFI	GTSAC	1 cut(s)	1854
TseI	GCWGC	9 cut(s)	34, 275, 1226, 1238, 1268, 1502, 1520, 2093, 2099
Tsp45I	GTSAC	1 cut(s)	1854
TspDTI	ATGAA	5 cut(s)	62, 219, 245, 602, 626
TspGWI	ACGGA	1 cut(s)	68
TspMI	CCCGGG	1 cut(s)	776
TspRI	CASTG	2 cut(s)	232, 795
Van91I	CCANNNNNTGG	1 cut(s)	1702
Vha464I	CTTAAG	1 cut(s)	158
VpaK11BI	GGWCC	3 cut(s)	286, 951, 1728
XagI	CCTNNNNNAGG	2 cut(s)	315, 1817
XapI	RAATTY	5 cut(s)	622, 683, 811, 1010, 1651
XbaI	TCTAGA	2 cut(s)	1039, 1645
XceI	RCATGY	2 cut(s)	1300, 2084
XmaI	CCCGGG	1 cut(s)	776
XmiI	GTMKAC	1 cut(s)	261
XspI	CTAG	8 cut(s)	482, 903, 1040, 1374, 1646, 1742, 1892, 2144
ZrmI	AGTACT	1 cut(s)	1470

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.