Rroxscaffold_2G00125140

Plant protein of unknown function (DUF936)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010333
Physical Location & Seq
Reverse (-)
59716061 .. 59723381
7321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00125140.1

Sequence Viewer

Length: 1011 bp
ATGGCCTTCACTCCCATCCTCACTTGCAAAGCTTGCAAAGGAAGTTTTGAGGCACAGGGATTCAGCACAAATGGCAGCAATAGAGGCAATGCAGGAGGCTTCTACTGCAGAAAGCTTGCTACGGTGTTTGAGGTAAGCATATATTCTGAGCTAATTACCACTGCAAAGGAAGATAATCCACAGCCTGCGGTAGAGCAGTTTTTAGCCCTCCATGCTAGCTTGAATAATGCTCGTCTAGTAGCTGATTCTCTATCCAAACTTACACCAGCTGGTTCATCCCCAGATCATGAAGAAAGCCCGTCAGAAGAAGTGCTAAAGGTTACATCAGAGAGGCGGAAACGGGCAACATCATGGGTACAAGCTGCATTAGCCACTAATTTGTCTTCCTTTGCTGTATTTAGTAAAGAGTCCTGTTCAACCTCAGTTCCGGCTTCAGCTTCCTCTCAAAACCAAAAGAATGTCTCTGCAAATCAACCCATCTTAGTTCTAGACAACTCTACCAAGAATGGCACAACAAAATCCCAGGGAAAAATTCGCCGGACAGTTGGCTCCAAGGTTGGTGCCTCACCAGGGACTCCTCGACGACTAGGGGATGGATCAGCCATCAGTCAAAAACCACAGACACAACCTCTACCTGAATGGATTAAAGGAAATGGCCTATATGAGACTGTCGACTTGGCTGAAATGTTGCGACTGCAGTCACAAGATTGGTTCTTGGGATTTGTAGAGAGGTTCCTGGATGCTGATGTGGACACTTCAGCTCTGTCAGATAATGGTCAAATATCAGGAATGCTAACTCAGCTCAAGAGTGTGAATGATTGGTTGGATGGTATTGGTTCTAATACAGATGCAGAAGAAGCAACTGCTGTTTCTGAAGAGACCATTGATAGGCTAAGGAAGAAGATCTACGAGTATCTTCTTACGCATGTTGAATCTGCTGCCGCTGCCCTTGGTGGTGGATCACAGTCATCACCGCGACTTCAAACAACAGATACAAAAGTTAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF936

Protein Analysis

336

Amino Acids

36.07

Weight (kDa)

6.37

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6857 PF21647 45 - 317 4e-77 Domain of unknown function (DUF6857)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 560
AccI GTMKAC 1 cut(s) 672
AccII CGCG 1 cut(s) 976
AciI CCGC 4 cut(s) 188, 334, 942, 974
AclWI GGATC 2 cut(s) 604, 967
AcsI RAATTY 1 cut(s) 531
AcuI CTGAAG 3 cut(s) 417, 741, 894
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 2 cut(s) 570, 888
AgsI TTSAA 4 cut(s) 223, 417, 932, 983
AjnI CCWGG 3 cut(s) 522, 568, 735
AjuI GAANNNNNNNTTGG 2 cut(s) 806, 838
Alw26I GTCTC 3 cut(s) 466, 659, 872
AlwI GGATC 2 cut(s) 604, 967
AoxI GGCC 2 cut(s) 3, 655
ApeKI GCWGC 4 cut(s) 75, 362, 938, 944
ApoI RAATTY 1 cut(s) 531
AsuHPI GGTGA 2 cut(s) 558, 963
AsuNHI GCTAGC 1 cut(s) 215
BaeI ACNNNNGTAYC 1 cut(s) 984
BanI GGYRCC 1 cut(s) 560
BarI GAAGNNNNNNTAC 2 cut(s) 890, 922
BbsI GAAGAC 1 cut(s) 375
BbvI GCAGC 4 cut(s) 87, 349, 925, 931
BccI CCATC 5 cut(s) 23, 485, 587, 611, 821
BciT130I CCWGG 3 cut(s) 524, 570, 737
BcoDI GTCTC 3 cut(s) 466, 659, 872
BfaI CTAG 4 cut(s) 216, 236, 488, 587
BfmI CTRYAG 2 cut(s) 106, 695
BglII AGATCT 1 cut(s) 903
BisI GCNGC 5 cut(s) 76, 363, 939, 942, 945
BlsI GCNGC 5 cut(s) 77, 364, 940, 943, 946
Bme1390I CCNGG 3 cut(s) 524, 570, 737
BmiI GGNNCC 3 cut(s) 550, 562, 734
BmrFI CCNGG 3 cut(s) 524, 570, 737
BmsI GCATC 2 cut(s) 730, 838
BmtI GCTAGC 1 cut(s) 219
BoxI GACNNNNGTC 1 cut(s) 697
BpiI GAAGAC 1 cut(s) 375
Bpu10I CCTNAGC 1 cut(s) 893
BpuEI CTTGAG 1 cut(s) 788
BsaI GGTCTC 1 cut(s) 872
BsaJI CCNNGG 5 cut(s) 522, 523, 552, 569, 949
Bsc4I CCNNNNNNNGG 2 cut(s) 570, 888
Bse3DI GCAATG 1 cut(s) 94
BseBI CCWGG 3 cut(s) 524, 570, 737
BseDI CCNNGG 5 cut(s) 522, 523, 552, 569, 949
BseGI GGATG 5 cut(s) 15, 275, 598, 745, 832
BseLI CCNNNNNNNGG 2 cut(s) 570, 888
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 3 cut(s) 138, 435, 812
BseRI GAGGAG 1 cut(s) 567
BseXI GCAGC 4 cut(s) 87, 349, 925, 931
Bsh1236I CGCG 1 cut(s) 976
BshFI GGCC 2 cut(s) 5, 657
BshNI GGYRCC 1 cut(s) 560
BsiSI CCGG 2 cut(s) 428, 538
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 2 cut(s) 570, 888
BsmAI GTCTC 3 cut(s) 466, 659, 872
BsmFI GGGAC 1 cut(s) 586
BsmI GAATGC 1 cut(s) 795
BsnI GGCC 2 cut(s) 5, 657
Bso31I GGTCTC 1 cut(s) 872
Bsp143I GATC 4 cut(s) 283, 596, 903, 959
BspACI CCGC 4 cut(s) 188, 334, 942, 974
BspANI GGCC 2 cut(s) 5, 657
BspCNI CTCAG 3 cut(s) 139, 434, 811
BspFNI CGCG 1 cut(s) 976
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 3 cut(s) 550, 562, 734
BspMAI CTGCAG 2 cut(s) 110, 699
BspOI GCTAGC 1 cut(s) 219
BspPI GGATC 2 cut(s) 604, 967
BspT107I GGYRCC 1 cut(s) 560
BspTNI GGTCTC 1 cut(s) 872
BsrDI GCAATG 1 cut(s) 94
BssECI CCNNGG 5 cut(s) 522, 523, 552, 569, 949
BssMI GATC 4 cut(s) 283, 596, 903, 959
BssT1I CCWWGG 2 cut(s) 552, 949
Bst2UI CCWGG 3 cut(s) 524, 570, 737
Bst4CI ACNGT 4 cut(s) 124, 544, 670, 966
Bst6I CTCTTC 1 cut(s) 870
BstAPI GCANNNNNTGC 1 cut(s) 33
BstC8I GCNNGC 4 cut(s) 34, 117, 186, 217
BstDEI CTNAG 5 cut(s) 147, 421, 481, 798, 893
BstF5I GGATG 5 cut(s) 15, 275, 598, 745, 832
BstFNI CGCG 1 cut(s) 976
BstKTI GATC 4 cut(s) 286, 599, 906, 962
BstMAI GTCTC 3 cut(s) 466, 659, 872
BstMBI GATC 4 cut(s) 283, 596, 903, 959
BstMWI GCNNNNNNNGC 9 cut(s) 33, 72, 84, 105, 212, 368, 799, 857, 944
BstNI CCWGG 3 cut(s) 524, 570, 737
BstNSI RCATGY 1 cut(s) 929
BstPAI GACNNNNGTC 1 cut(s) 697
BstSCI CCNGG 3 cut(s) 522, 568, 735
BstSFI CTRYAG 2 cut(s) 106, 695
BstUI CGCG 1 cut(s) 976
BstV1I GCAGC 4 cut(s) 87, 349, 925, 931
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 903
BstYI RGATCY 1 cut(s) 903
BsuRI GGCC 2 cut(s) 5, 657
BtsCI GGATG 5 cut(s) 15, 275, 598, 745, 832
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 4 cut(s) 34, 117, 186, 217
CciI TCATGA 1 cut(s) 286
Csp6I GTAC 1 cut(s) 356
CviAII CATG 4 cut(s) 212, 287, 351, 926
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 5 cut(s) 147, 421, 481, 798, 893
DpnI GATC 4 cut(s) 285, 598, 905, 961
DpnII GATC 4 cut(s) 283, 596, 903, 959
Eam1104I CTCTTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 870
EciI GGCGGA 1 cut(s) 349
Eco130I CCWWGG 2 cut(s) 552, 949
Eco31I GGTCTC 1 cut(s) 872
Eco57I CTGAAG 3 cut(s) 417, 741, 894
EcoRII CCWGG 3 cut(s) 522, 568, 735
EcoT14I CCWWGG 2 cut(s) 552, 949
ErhI CCWWGG 2 cut(s) 552, 949
FaeI CATG 4 cut(s) 215, 290, 354, 929
FaiI YATR 8 cut(s) 140, 142, 213, 288, 352, 661, 663, 927
FaqI GGGAC 1 cut(s) 586
FatI CATG 4 cut(s) 211, 286, 350, 925
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 5 cut(s) 76, 363, 939, 942, 945
FokI GGATG 5 cut(s) 2, 262, 605, 752, 839
Fsp4HI GCNGC 5 cut(s) 76, 363, 939, 942, 945
FspBI CTAG 4 cut(s) 216, 236, 488, 587
GluI GCNGC 5 cut(s) 76, 363, 939, 942, 945
HaeIII GGCC 2 cut(s) 5, 657
HapII CCGG 2 cut(s) 428, 538
Hin1II CATG 4 cut(s) 215, 290, 354, 929
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 2 cut(s) 30, 113
HinfI GANTC 5 cut(s) 60, 245, 407, 574, 932
HpaII CCGG 2 cut(s) 428, 538
HphI GGTGA 2 cut(s) 558, 963
Hpy166II GTNNAC 2 cut(s) 673, 751
Hpy188I TCNGA 5 cut(s) 148, 304, 328, 769, 874
Hpy188III TCNNGA 4 cut(s) 287, 488, 786, 805
Hpy8I GTNNAC 2 cut(s) 673, 751
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 4 cut(s) 124, 544, 670, 966
HpyCH4V TGCA 9 cut(s) 27, 36, 92, 108, 164, 365, 467, 697, 851
HpyF10VI GCNNNNNNNGC 9 cut(s) 33, 72, 84, 105, 212, 368, 799, 857, 944
HpyF3I CTNAG 5 cut(s) 147, 421, 481, 798, 893
Hsp92II CATG 4 cut(s) 215, 290, 354, 929
Kzo9I GATC 4 cut(s) 283, 596, 903, 959
LmnI GCTCC 1 cut(s) 554
Lsp1109I GCAGC 4 cut(s) 87, 349, 925, 931
LweI GCATC 2 cut(s) 730, 838
MaeI CTAG 4 cut(s) 216, 236, 488, 587
MaeIII GTNAC 2 cut(s) 319, 699
MalI GATC 4 cut(s) 285, 598, 905, 961
MboI GATC 4 cut(s) 283, 596, 903, 959
MboII GAAGA 9 cut(s) 182, 302, 317, 375, 866, 887, 908, 910, 913
MflI RGATCY 1 cut(s) 903
MluCI AATT 3 cut(s) 153, 376, 531
MlyI GAGTC 2 cut(s) 416, 568
MmeI TCCRAC 1 cut(s) 804
MseI TTAA 1 cut(s) 645
MspA1I CMGCKG 2 cut(s) 269, 944
MspI CCGG 2 cut(s) 428, 538
MspR9I CCNGG 3 cut(s) 524, 570, 737
Mva1269I GAATGC 1 cut(s) 795
MvaI CCWGG 3 cut(s) 524, 570, 737
MvnI CGCG 1 cut(s) 976
MwoI GCNNNNNNNGC 9 cut(s) 33, 72, 84, 105, 212, 368, 799, 857, 944
NdeII GATC 4 cut(s) 283, 596, 903, 959
NheI GCTAGC 1 cut(s) 215
NlaIII CATG 4 cut(s) 215, 290, 354, 929
NlaIV GGNNCC 3 cut(s) 550, 562, 734
NmuCI GTSAC 1 cut(s) 699
NspI RCATGY 1 cut(s) 929
PagI TCATGA 1 cut(s) 286
PasI CCCWGGG 1 cut(s) 523
PctI GAATGC 1 cut(s) 795
PfeI GAWTC 3 cut(s) 60, 245, 932
PfoI TCCNGGA 1 cut(s) 735
PkrI GCNGC 5 cut(s) 77, 364, 940, 943, 946
PleI GAGTC 2 cut(s) 415, 568
PpsI GAGTC 2 cut(s) 415, 568
PshAI GACNNNNGTC 1 cut(s) 697
Psp6I CCWGG 3 cut(s) 522, 568, 735
PspGI CCWGG 3 cut(s) 522, 568, 735
PspN4I GGNNCC 3 cut(s) 550, 562, 734
PstI CTGCAG 2 cut(s) 110, 699
PsuI RGATCY 1 cut(s) 903
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
SalI GTCGAC 1 cut(s) 671
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 5 cut(s) 76, 363, 939, 942, 945
Sau3AI GATC 4 cut(s) 283, 596, 903, 959
SchI GAGTC 2 cut(s) 416, 568
ScrFI CCNGG 3 cut(s) 524, 570, 737
SfaNI GCATC 2 cut(s) 730, 838
SfcI CTRYAG 2 cut(s) 106, 695
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
Sse9I AATT 3 cut(s) 153, 376, 531
SsiI CCGC 4 cut(s) 188, 334, 942, 974
SspMI CTAG 4 cut(s) 216, 236, 488, 587
StyD4I CCNGG 3 cut(s) 522, 568, 735
StyI CCWWGG 2 cut(s) 552, 949
TaaI ACNGT 4 cut(s) 124, 544, 670, 966
TaqI TCGA 2 cut(s) 580, 672
TasI AATT 3 cut(s) 153, 376, 531
TauI GCSGC 1 cut(s) 944
TfiI GAWTC 3 cut(s) 60, 245, 932
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 699
TseI GCWGC 4 cut(s) 75, 362, 938, 944
Tsp45I GTSAC 1 cut(s) 699
TspDTI ATGAA 2 cut(s) 264, 303
TspRI CASTG 1 cut(s) 166
XapI RAATTY 1 cut(s) 531
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 1 cut(s) 929
XmiI GTMKAC 1 cut(s) 672
XspI CTAG 4 cut(s) 216, 236, 488, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.