Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0015181

Physical Location & Seq

Genomic Coordinates Reverse (-)

36968490 .. 36974799

Gene Structure 6310 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1273200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1902 bp

ATGGGTGCTCCCTTGTCTGCTTGGCCTTGGGAATACCTAGGCTCCTTCAAGTATGTGCTGTTCGGACCTTTGATCGGAAAAGTCTTGTATTCAATAATCCATGAGAAGGAGGATCCCCTCACACTACTCACCACCAGCTGGTGTCTCCATATTCTCCTTCTATGCATACTTCGAGCCGTAATTCACCAGCTATGGTCTAGTTACACCAACATGCTCTTCCTCACAAGAAATCGCCGGATCATCCAACAGGGGGTTGATTTCAAGCAGATTGACAAAGAATGGCACTGGGATAACTTCATAATTCTTCACGGTCTGATTGCCTCCATGGTCTGCTGTTTGGTGCCATCATCTCTGCAAAACCTACCCTTGTGGAGTACAAGAGGATTTATTACCACTCTGGTACTTCACGTGGGAGTTTCAGAACCTTTATATTACTGGGTGCACAAACGTTTTCACAGCAACAACTACTTTTTTGACAATTACCATTCACTTCACCATTCATCTCCAGTACCACAGACCTCTACCGCTGGAAATGCAACCCTGTTGGAACATCTGATATTGAGCATAACTATTGGAATTCCAGTTTTTGGTACGAATCTGATGGGTTTTGGATCAGTGTGCATGATCTATGGCTACGTTTTGGTTTTTGATTTTCTGAGATGTTTGGGGCATTCCAATGTTGAGGTCGTTCCTCATCAACTCTTTGAGAAAAAACCGTTCCTCAGATATCTTCTCTATACTCCAACATACCACAGCCTACACCATACTGAAATGGACACCAACTTCTGCCTTTTCATGCCTCTCTTTGATGCACTGGGGAAGACACTAAACGCAAAATCCTGGGGACTACACAAACAGATTAGCTTGAATGCAGGTAAAAATGGCAAGGCACCGGATTTCGTTTTCCTCGCGCATGTGGTGGATCTGTCGTCGTCTTTGCACGTGCCGTTTGTTTTCCGATCATTCTCATCGATTCCATTCTCAATGAGATTCTTCTTAGTGTTTGGTTGGCTTCCAGGCATTGTAGCCATGCTAGCAATGTGGGCATGGGCTAAAACTTTCCTGATTTCTTTTTACAACCTCAGAGACCGCTTGCACCAGACATGGGCTGTGCCTCGATACGGCTTTCAGTATTTTTTACCATTTGCTCTAGATGGCATCAATAATCAAATAGAGCAAGCAATCCTTAGAGCTGATAGAATGGGGGTCAAGGTCATCAGCCTTGCAGCATTAAATAAGAACGAAGGACTGAATGGGGGCGGAACGCTCTTTGTCAACAAGCATCCAGACCTTAGAGTTCGAGTGGTCCATGGCAACACATTAACTGCTGCTGTCATTCTCAACGAGATTCCAAAGGATGTCAAGGAGGTTTTTCTTAGTGGTGCTACCTCCAAGCTTGGAAGAGCTATTGCCCTCTATCTTTGCCGTAGGAGAGTACGCGTTCTGATGCTGACTCTATCAACGGAAAGATTTCAAAAAATTCAGAAGGAAGCGCCAGCAGATTATCAACAATACCTTGTGCAAGTAACAAAGTACCATGCCGCACAAAATTGCAAGACATGGATTGTGGGGAAATGGATCACACCAAGAGAGCAACGTTGGGCCCCAAGTGGAACTCATTTCCATCAGTTCGTGGTTCCACCCATCTTGCAGTTTAGAAAAGACTGCACTTACGGTGATCTTGCAGCCATGAGGTTACCTGATGACGTTGAAGGCCTTGGCATCTGCGAGTACACGATGGACAGAGGAGTAGTGCATGCATGCCATGCGGGAGGCGTTGTTCATTACCTGGAAGGTTGGACTCACCACGAAGTCGGGGCTCTGGATGTTGACAGAATTGACCTTGTATGGAATGCTGCTCTTAAACACGGTTTAAAGCCTGTTTCATCGAAGCCAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006629 GO:0006723 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009058 GO:0009555 GO:0009987 GO:0010025 GO:0010166 GO:0012505 GO:0016020 GO:0016491 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0042175 GO:0042335 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043446 GO:0043447 GO:0044237 GO:0044238 GO:0044249 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0055114 GO:0071704 GO:0071944 GO:0098827 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FA_hydroxylase Wax2_C

Protein Analysis

634

Amino Acids

72.16

Weight (kDa)

9.13

Isoelectric Point (pI)

31.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FA_hydroxylase	PF04116	134 - 275	1.6e-19	Fatty acid hydroxylase
CER1-like_C	PF12076	457 - 627	2e-69	CER1-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57800
fragaria_vesca	FvH4_4g10190
malus_domestica	MD13G1273200.v1.1
prunus_persica	Prupe.1G098000_v2.0.a1
pyrus_communis	pycom13g28860
rosa_chinensis	RchiOBHm_Chr4g0404991
rosa_laevigata	RLG00000008887
rosa_multiflora	Rmu_ssc0000127.1_g000007
rosa_roxburghii	Rroxscaffold_5G00349160
rosa_rugosa	Rorug04G0051000 Rorug04G0051100
rosa_samantha	Rh4AG126700 Rh4BG120900 Rh4CG134900 Rh4DG121200
rosa_wichuraiana	Rw4G010300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	863
AccB1I	GGYRCC	2 cut(s)	340, 889
AccB7I	CCANNNNNTGG	2 cut(s)	138, 587
AccII	CGCG	2 cut(s)	911, 1440
AciI	CCGC	5 cut(s)	525, 1090, 1260, 1542, 1769
AclI	AACGTT	2 cut(s)	448, 1597
AclWI	GGATC	6 cut(s)	107, 120, 245, 619, 930, 1586
AcsI	RAATTY	2 cut(s)	576, 1479
AcvI	CACGTG	2 cut(s)	409, 943
AfaI	GTAC	7 cut(s)	376, 402, 510, 592, 1437, 1535, 1733
AfiI	CCNNNNNNNGG	7 cut(s)	74, 106, 138, 250, 587, 1105, 1121
AflIII	ACRYGT	1 cut(s)	1438
AgsI	TTSAA	6 cut(s)	49, 93, 262, 868, 1475, 1712
AjnI	CCWGG	3 cut(s)	839, 1015, 1788
AjuI	GAANNNNNNNTTGG	2 cut(s)	200, 232
AloI	GAACNNNNNNTCC	2 cut(s)	1764, 1796
AluBI	AGCT	6 cut(s)	138, 190, 864, 1193, 1396, 1406
AluI	AGCT	6 cut(s)	138, 190, 864, 1193, 1396, 1406
Alw21I	GWGCWC	2 cut(s)	10, 444
Alw26I	GTCTC	2 cut(s)	149, 1080
Alw44I	GTGCAC	1 cut(s)	440
AlwI	GGATC	6 cut(s)	107, 120, 245, 619, 930, 1586
AoxI	GGCC	3 cut(s)	23, 1602, 1714
ApaI	GGGCCC	1 cut(s)	1606
ApaLI	GTGCAC	1 cut(s)	440
ApeKI	GCWGC	4 cut(s)	1226, 1328, 1685, 1856
ApoI	RAATTY	2 cut(s)	576, 1479
Asp700I	GAANNNNTTC	1 cut(s)	1470
AspA2I	CCTAGG	1 cut(s)	37
AspLEI	GCGC	2 cut(s)	913, 1495
AspS9I	GGNCC	4 cut(s)	65, 1306, 1602, 1603
AsuHPI	GGTGA	5 cut(s)	121, 176, 485, 1688, 1796
AsuNHI	GCTAGC	1 cut(s)	1033
AvaII	GGWCC	2 cut(s)	65, 1306
AvrII	CCTAGG	1 cut(s)	37
BaeGI	GKGCMC	2 cut(s)	444, 1606
BamHI	GGATCC	1 cut(s)	112
BanI	GGYRCC	2 cut(s)	340, 889
BanII	GRGCYC	2 cut(s)	1606, 1822
BbrPI	CACGTG	2 cut(s)	409, 943
BbsI	GAAGAC	1 cut(s)	827
Bbv12I	GWGCWC	2 cut(s)	10, 444
BbvI	GCAGC	4 cut(s)	1238, 1315, 1697, 1843
BccI	CCATC	6 cut(s)	352, 595, 1148, 1632, 1652, 1732
BceAI	ACGGC	4 cut(s)	161, 931, 1138, 1410
BciT130I	CCWGG	3 cut(s)	841, 1017, 1790
BcoDI	GTCTC	2 cut(s)	149, 1080
BfaI	CTAG	4 cut(s)	38, 198, 1034, 1151
BfoI	RGCGCY	1 cut(s)	1496
BfuAI	ACCTGC	1 cut(s)	863
BisI	GCNGC	5 cut(s)	1227, 1329, 1542, 1686, 1857
BlnI	CCTAGG	1 cut(s)	37
BlsI	GCNGC	5 cut(s)	1228, 1330, 1543, 1687, 1858
Bme1390I	CCNGG	3 cut(s)	841, 1017, 1790
Bme18I	GGWCC	2 cut(s)	65, 1306
BmgT120I	GGNCC	4 cut(s)	65, 1306, 1602, 1603
BmiI	GGNNCC	7 cut(s)	43, 114, 342, 891, 1604, 1605, 1638
BmrFI	CCNGG	3 cut(s)	841, 1017, 1790
BmrI	ACTGGG	3 cut(s)	295, 445, 824
BmsI	GCATC	5 cut(s)	799, 1167, 1291, 1437, 1731
BmtI	GCTAGC	1 cut(s)	1037
BmuI	ACTGGG	3 cut(s)	295, 445, 824
BpiI	GAAGAC	1 cut(s)	827
BpmI	CTGGAG	1 cut(s)	489
Bsa29I	ATCGAT	1 cut(s)	971
BsaAI	YACGTR	2 cut(s)	409, 943
BsaI	GGTCTC	1 cut(s)	1080
BsaJI	CCNNGG	6 cut(s)	26, 37, 324, 840, 1309, 1717
BsaWI	WCCGGW	1 cut(s)	892
BsaXI	ACNNNNNCTCC	4 cut(s)	26, 56, 1764, 1794
Bsc4I	CCNNNNNNNGG	7 cut(s)	74, 106, 138, 250, 587, 1105, 1121
Bse1I	ACTGG	5 cut(s)	290, 440, 506, 581, 819
Bse3DI	GCAATG	1 cut(s)	1044
BseBI	CCWGG	3 cut(s)	841, 1017, 1790
BseCI	ATCGAT	1 cut(s)	971
BseDI	CCNNGG	6 cut(s)	26, 37, 324, 840, 1309, 1717
BseGI	GGATG	4 cut(s)	240, 1282, 1363, 1831
BseLI	CCNNNNNNNGG	7 cut(s)	74, 106, 138, 250, 587, 1105, 1121
BseMI	GCAATG	1 cut(s)	1044
BseMII	CTCAG	3 cut(s)	647, 736, 1096
BseNI	ACTGG	5 cut(s)	290, 440, 506, 581, 819
BseRI	GAGGAG	1 cut(s)	1761
BseSI	GKGCMC	2 cut(s)	444, 1606
BseXI	GCAGC	4 cut(s)	1238, 1315, 1697, 1843
BsgI	GTGCAG	1 cut(s)	1651
Bsh1236I	CGCG	2 cut(s)	911, 1440
BshFI	GGCC	3 cut(s)	25, 1604, 1716
BshNI	GGYRCC	2 cut(s)	340, 889
BshVI	ATCGAT	1 cut(s)	971
BsiHKAI	GWGCWC	2 cut(s)	10, 444
BsiSI	CCGG	2 cut(s)	235, 893
BslFI	GGGAC	1 cut(s)	858
BslI	CCNNNNNNNGG	7 cut(s)	74, 106, 138, 250, 587, 1105, 1121
BsmAI	GTCTC	2 cut(s)	149, 1080
BsmFI	GGGAC	1 cut(s)	858
BsmI	GAATGC	3 cut(s)	670, 874, 1858
BsnI	GGCC	3 cut(s)	25, 1604, 1716
Bso31I	GGTCTC	1 cut(s)	1080
Bsp120I	GGGCCC	1 cut(s)	1602
Bsp1286I	GDGCHC	4 cut(s)	10, 444, 1606, 1822
Bsp143I	GATC	9 cut(s)	72, 112, 237, 611, 624, 922, 959, 1578, 1678
Bsp19I	CCATGG	2 cut(s)	324, 1309
BspACI	CCGC	5 cut(s)	525, 1090, 1260, 1542, 1769
BspANI	GGCC	3 cut(s)	25, 1604, 1716
BspCNI	CTCAG	3 cut(s)	648, 735, 1095
BspDI	ATCGAT	1 cut(s)	971
BspFNI	CGCG	2 cut(s)	911, 1440
BspLI	GGNNCC	7 cut(s)	43, 114, 342, 891, 1604, 1605, 1638
BspMI	ACCTGC	1 cut(s)	863
BspOI	GCTAGC	1 cut(s)	1037
BspPI	GGATC	6 cut(s)	107, 120, 245, 619, 930, 1586
BspQI	GCTCTTC	2 cut(s)	221, 1396
BspT107I	GGYRCC	2 cut(s)	340, 889
BspTNI	GGTCTC	1 cut(s)	1080
BsrDI	GCAATG	1 cut(s)	1044
BsrI	ACTGG	5 cut(s)	290, 440, 506, 581, 819
BssECI	CCNNGG	6 cut(s)	26, 37, 324, 840, 1309, 1717
BssMI	GATC	9 cut(s)	72, 112, 237, 611, 624, 922, 959, 1578, 1678
BssT1I	CCWWGG	5 cut(s)	26, 37, 324, 1309, 1717
Bst2UI	CCWGG	3 cut(s)	841, 1017, 1790
Bst4CI	ACNGT	4 cut(s)	311, 717, 1676, 1871
Bst6I	CTCTTC	2 cut(s)	221, 1396
BstAPI	GCANNNNNTGC	1 cut(s)	1766
BstBAI	YACGTR	2 cut(s)	409, 943
BstC8I	GCNNGC	6 cut(s)	1035, 1094, 1179, 1497, 1758, 1762
BstDEI	CTNAG	7 cut(s)	656, 722, 997, 1082, 1187, 1292, 1376
BstDSI	CCRYGG	2 cut(s)	324, 1309
BstEII	GGTNACC	1 cut(s)	1695
BstF5I	GGATG	4 cut(s)	240, 1282, 1363, 1831
BstFNI	CGCG	2 cut(s)	911, 1440
BstH2I	RGCGCY	1 cut(s)	1496
BstHHI	GCGC	2 cut(s)	913, 1495
BstKTI	GATC	9 cut(s)	75, 115, 240, 614, 627, 925, 962, 1581, 1681
BstMAI	GTCTC	2 cut(s)	149, 1080
BstMBI	GATC	9 cut(s)	72, 112, 237, 611, 624, 922, 959, 1578, 1678
BstMWI	GCNNNNNNNGC	4 cut(s)	533, 1034, 1043, 1766
BstNI	CCWGG	3 cut(s)	841, 1017, 1790
BstNSI	RCATGY	4 cut(s)	214, 917, 1760, 1764
BstPI	GGTNACC	1 cut(s)	1695
BstSCI	CCNGG	3 cut(s)	839, 1015, 1788
BstSLI	GKGCMC	2 cut(s)	444, 1606
BstUI	CGCG	2 cut(s)	911, 1440
BstV1I	GCAGC	4 cut(s)	1238, 1315, 1697, 1843
BstV2I	GAAGAC	1 cut(s)	827
BstX2I	RGATCY	2 cut(s)	112, 922
BstYI	RGATCY	2 cut(s)	112, 922
Bsu15I	ATCGAT	1 cut(s)	971
BsuRI	GGCC	3 cut(s)	25, 1604, 1716
BsuTUI	ATCGAT	1 cut(s)	971
BtgI	CCRYGG	2 cut(s)	324, 1309
BtsCI	GGATG	4 cut(s)	240, 1282, 1363, 1831
BtsIMutI	CAGTG	3 cut(s)	283, 621, 812
BveI	ACCTGC	1 cut(s)	863
Cac8I	GCNNGC	6 cut(s)	1035, 1094, 1179, 1497, 1758, 1762
CfoI	GCGC	2 cut(s)	913, 1495
Cfr13I	GGNCC	4 cut(s)	65, 1306, 1602, 1603
ClaI	ATCGAT	1 cut(s)	971
Csp6I	GTAC	7 cut(s)	375, 401, 509, 591, 1436, 1534, 1732
CspCI	CAANNNNNGTGG	2 cut(s)	1629, 1664
CviQI	GTAC	7 cut(s)	375, 401, 509, 591, 1436, 1534, 1732
DdeI	CTNAG	7 cut(s)	656, 722, 997, 1082, 1187, 1292, 1376
DpnI	GATC	9 cut(s)	74, 114, 239, 613, 626, 924, 961, 1580, 1680
DpnII	GATC	9 cut(s)	72, 112, 237, 611, 624, 922, 959, 1578, 1678
DraI	TTTAAA	1 cut(s)	1875
Eam1104I	CTCTTC	2 cut(s)	221, 1396
EarI	CTCTTC	2 cut(s)	221, 1396
EciI	GGCGGA	1 cut(s)	1275
Eco130I	CCWWGG	5 cut(s)	26, 37, 324, 1309, 1717
Eco147I	AGGCCT	1 cut(s)	1716
Eco24I	GRGCYC	2 cut(s)	1606, 1822
Eco31I	GGTCTC	1 cut(s)	1080
Eco32I	GATATC	1 cut(s)	728
Eco47I	GGWCC	2 cut(s)	65, 1306
Eco72I	CACGTG	2 cut(s)	409, 943
Eco91I	GGTNACC	1 cut(s)	1695
EcoO109I	RGGNCCY	1 cut(s)	1603
EcoO65I	GGTNACC	1 cut(s)	1695
EcoRI	GAATTC	1 cut(s)	576
EcoRII	CCWGG	3 cut(s)	839, 1015, 1788
EcoRV	GATATC	1 cut(s)	728
EcoT14I	CCWWGG	5 cut(s)	26, 37, 324, 1309, 1717
EcoT22I	ATGCAT	2 cut(s)	167, 1762
EcoT38I	GRGCYC	2 cut(s)	1606, 1822
ErhI	CCWWGG	5 cut(s)	26, 37, 324, 1309, 1717
FalI	AAGNNNNNCTT	2 cut(s)	1170, 1202
FaqI	GGGAC	1 cut(s)	858
FauI	CCCGC	1 cut(s)	1762
Fnu4HI	GCNGC	5 cut(s)	1227, 1329, 1542, 1686, 1857
FokI	GGATG	4 cut(s)	227, 1269, 1370, 1838
FriOI	GRGCYC	2 cut(s)	1606, 1822
Fsp4HI	GCNGC	5 cut(s)	1227, 1329, 1542, 1686, 1857
FspBI	CTAG	4 cut(s)	38, 198, 1034, 1151
GlaI	GCGC	2 cut(s)	912, 1494
GluI	GCNGC	5 cut(s)	1227, 1329, 1542, 1686, 1857
GsuI	CTGGAG	1 cut(s)	489
HaeII	RGCGCY	1 cut(s)	1496
HaeIII	GGCC	3 cut(s)	25, 1604, 1716
HapII	CCGG	2 cut(s)	235, 893
HhaI	GCGC	2 cut(s)	913, 1495
Hin6I	GCGC	2 cut(s)	911, 1493
HinP1I	GCGC	2 cut(s)	911, 1493
HincII	GTYRAC	2 cut(s)	1276, 1831
HindII	GTYRAC	2 cut(s)	1276, 1831
HindIII	AAGCTT	1 cut(s)	1394
HinfI	GANTC	6 cut(s)	595, 973, 990, 1348, 1453, 1801
HpaII	CCGG	2 cut(s)	235, 893
HphI	GGTGA	5 cut(s)	121, 176, 485, 1688, 1796
Hpy166II	GTNNAC	4 cut(s)	442, 1276, 1734, 1831
Hpy188III	TCNNGA	4 cut(s)	1063, 1151, 1286, 1823
Hpy8I	GTNNAC	4 cut(s)	442, 1276, 1734, 1831
Hpy99I	CGWCG	1 cut(s)	934
HpyAV	CCTTC	7 cut(s)	55, 100, 167, 1238, 1480, 1706, 1787
HpyCH4III	ACNGT	4 cut(s)	311, 717, 1676, 1871
HpyCH4IV	ACGT	6 cut(s)	408, 448, 636, 942, 1597, 1707
HpyF10VI	GCNNNNNNNGC	4 cut(s)	533, 1034, 1043, 1766
HpyF3I	CTNAG	7 cut(s)	656, 722, 997, 1082, 1187, 1292, 1376
HpySE526I	ACGT	6 cut(s)	408, 448, 636, 942, 1597, 1707
HspAI	GCGC	2 cut(s)	911, 1493
Kzo9I	GATC	9 cut(s)	72, 112, 237, 611, 624, 922, 959, 1578, 1678
LguI	GCTCTTC	2 cut(s)	221, 1396
LmnI	GCTCC	2 cut(s)	13, 47
Lsp1109I	GCAGC	4 cut(s)	1238, 1315, 1697, 1843
LweI	GCATC	5 cut(s)	799, 1167, 1291, 1437, 1731
MaeI	CTAG	4 cut(s)	38, 198, 1034, 1151
MaeII	ACGT	6 cut(s)	408, 448, 636, 942, 1597, 1707
MaeIII	GTNAC	3 cut(s)	200, 1525, 1695
MalI	GATC	9 cut(s)	74, 114, 239, 613, 626, 924, 961, 1580, 1680
MboI	GATC	9 cut(s)	72, 112, 237, 611, 624, 922, 959, 1578, 1678
MboII	GAAGA	6 cut(s)	208, 296, 722, 832, 985, 1413
MflI	RGATCY	2 cut(s)	112, 922
MhlI	GDGCHC	4 cut(s)	10, 444, 1606, 1822
MluCI	AATT	7 cut(s)	180, 300, 478, 576, 1479, 1549, 1836
MluI	ACGCGT	1 cut(s)	1438
MlyI	GAGTC	2 cut(s)	1447, 1795
MmeI	TCCRAC	4 cut(s)	268, 525, 767, 1778
Mph1103I	ATGCAT	2 cut(s)	167, 1762
MroXI	GAANNNNTTC	1 cut(s)	1470
MseI	TTAA	5 cut(s)	1232, 1322, 1863, 1874, 1900
MslI	CAYNNNNRTG	2 cut(s)	209, 675
MspA1I	CMGCKG	2 cut(s)	138, 527
MspI	CCGG	2 cut(s)	235, 893
MspR9I	CCNGG	3 cut(s)	841, 1017, 1790
Mva1269I	GAATGC	3 cut(s)	670, 874, 1858
MvaI	CCWGG	3 cut(s)	841, 1017, 1790
MvnI	CGCG	2 cut(s)	911, 1440
MwoI	GCNNNNNNNGC	4 cut(s)	533, 1034, 1043, 1766
NcoI	CCATGG	2 cut(s)	324, 1309
NdeII	GATC	9 cut(s)	72, 112, 237, 611, 624, 922, 959, 1578, 1678
NheI	GCTAGC	1 cut(s)	1033
NlaIV	GGNNCC	7 cut(s)	43, 114, 342, 891, 1604, 1605, 1638
NsiI	ATGCAT	2 cut(s)	167, 1762
NspI	RCATGY	4 cut(s)	214, 917, 1760, 1764
PaeI	GCATGC	2 cut(s)	1760, 1764
PceI	AGGCCT	1 cut(s)	1716
PciSI	GCTCTTC	2 cut(s)	221, 1396
PctI	GAATGC	3 cut(s)	670, 874, 1858
PdmI	GAANNNNTTC	1 cut(s)	1470
PfeI	GAWTC	4 cut(s)	595, 973, 990, 1348
PflMI	CCANNNNNTGG	2 cut(s)	138, 587
PkrI	GCNGC	5 cut(s)	1228, 1330, 1543, 1687, 1858
PleI	GAGTC	2 cut(s)	1447, 1795
PmaCI	CACGTG	2 cut(s)	409, 943
PmlI	CACGTG	2 cut(s)	409, 943
PpsI	GAGTC	2 cut(s)	1447, 1795
Ppu21I	YACGTR	2 cut(s)	409, 943
Psp1406I	AACGTT	2 cut(s)	448, 1597
Psp6I	CCWGG	3 cut(s)	839, 1015, 1788
PspCI	CACGTG	2 cut(s)	409, 943
PspEI	GGTNACC	1 cut(s)	1695
PspGI	CCWGG	3 cut(s)	839, 1015, 1788
PspN4I	GGNNCC	7 cut(s)	43, 114, 342, 891, 1604, 1605, 1638
PspOMI	GGGCCC	1 cut(s)	1602
PspPI	GGNCC	4 cut(s)	65, 1306, 1602, 1603
PsrI	GAACNNNNNNTAC	2 cut(s)	44, 76
PsuI	RGATCY	2 cut(s)	112, 922
PvuII	CAGCTG	1 cut(s)	138
RsaI	GTAC	7 cut(s)	376, 402, 510, 592, 1437, 1535, 1733
RsaNI	GTAC	7 cut(s)	375, 401, 509, 591, 1436, 1534, 1732
RseI	CAYNNNNRTG	2 cut(s)	209, 675
SapI	GCTCTTC	2 cut(s)	221, 1396
SaqAI	TTAA	5 cut(s)	1232, 1322, 1863, 1874, 1900
SatI	GCNGC	5 cut(s)	1227, 1329, 1542, 1686, 1857
Sau3AI	GATC	9 cut(s)	72, 112, 237, 611, 624, 922, 959, 1578, 1678
Sau96I	GGNCC	4 cut(s)	65, 1306, 1602, 1603
SchI	GAGTC	2 cut(s)	1447, 1795
ScrFI	CCNGG	3 cut(s)	841, 1017, 1790
SduI	GDGCHC	4 cut(s)	10, 444, 1606, 1822
SfaNI	GCATC	5 cut(s)	799, 1167, 1291, 1437, 1731
SinI	GGWCC	2 cut(s)	65, 1306
SmiMI	CAYNNNNRTG	2 cut(s)	209, 675
SphI	GCATGC	2 cut(s)	1760, 1764
Sse9I	AATT	7 cut(s)	180, 300, 478, 576, 1479, 1549, 1836
SseBI	AGGCCT	1 cut(s)	1716
SsiI	CCGC	5 cut(s)	525, 1090, 1260, 1542, 1769
SspMI	CTAG	4 cut(s)	38, 198, 1034, 1151
StuI	AGGCCT	1 cut(s)	1716
StyD4I	CCNGG	3 cut(s)	839, 1015, 1788
StyI	CCWWGG	5 cut(s)	26, 37, 324, 1309, 1717
TaaI	ACNGT	4 cut(s)	311, 717, 1676, 1871
TaiI	ACGT	6 cut(s)	411, 451, 639, 945, 1600, 1710
TaqI	TCGA	5 cut(s)	172, 971, 1117, 1300, 1889
TasI	AATT	7 cut(s)	180, 300, 478, 576, 1479, 1549, 1836
TatI	WGTACW	2 cut(s)	374, 1731
TauI	GCSGC	1 cut(s)	1544
TfiI	GAWTC	4 cut(s)	595, 973, 990, 1348
Tru1I	TTAA	5 cut(s)	1232, 1322, 1863, 1874, 1900
Tru9I	TTAA	5 cut(s)	1232, 1322, 1863, 1874, 1900
TscAI	CASTG	3 cut(s)	290, 621, 819
TseI	GCWGC	4 cut(s)	1226, 1328, 1685, 1856
TspDTI	ATGAA	5 cut(s)	286, 489, 784, 1772, 1875
TspGWI	ACGGA	1 cut(s)	1478
TspRI	CASTG	3 cut(s)	290, 621, 819
Van91I	CCANNNNNTGG	2 cut(s)	138, 587
VneI	GTGCAC	1 cut(s)	440
VpaK11BI	GGWCC	2 cut(s)	65, 1306
XapI	RAATTY	2 cut(s)	576, 1479
XbaI	TCTAGA	1 cut(s)	1150
XceI	RCATGY	4 cut(s)	214, 917, 1760, 1764
XmaJI	CCTAGG	1 cut(s)	37
XmnI	GAANNNNTTC	1 cut(s)	1470
XspI	CTAG	4 cut(s)	38, 198, 1034, 1151
Zsp2I	ATGCAT	2 cut(s)	167, 1762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.