Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0013446

Physical Location & Seq

Genomic Coordinates Forward (+)

1478858 .. 1480465

Gene Structure 1608 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1016400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGAGCTGCTGCTGCCCTTTTGTTTCCCTCCCATGGCTGCTTTTGTTCTGTAGTCATCACTCTCTCTCTCCCTCTCCCTCTCTCTCTCTGATATTCATTTCCACTATGGAAACGTTTACCAAATCAGTGACTCGTCTGAGCTCAGCTCAGCTTCTTCTAATAACACTCACTGTATTACTCTACGTCACATCGCCACCTTACGTCGCCGCCGCCCAAGCGGAGCTTCCCAGAGGCTTCAAAGCCGCTCCGGACCCTTCAATCCAAACCTTCCAACCTCTGCTCACCGACCCAACCGGCAACTTCTCACTGGGTTTCCTCCGAGTCAACAAAACCCAACTAGCTCTGGCTCTCCTCCACGTGCCTTCCTCCGACCCGTTTTGGCTCGCCAACCCGACCCAACTCGCCCGCTGGTCCGACCGCACCACCCTCCTCTTCAACGGCAGCCTCGTCCTCTTAGATTCCCAGTCGAGAGTATTCTGGTCAACTCACACCGAAACCGGCGATCGGGTTGCAATCCTCGACACCTCCAACGTCCAAATCCAAACAACTCAGGACAACGTTCTATGGCAGAGTTTTGACGTTCCCACAAATACCCTCGTCGAGAACCAGAATTTCACATCGACCATGAAGCTTGTCTCATCCAACGGCCTATATTCGATGCAATTGGGCAACGATTTCATGGGTCTGTACGCGAAGTTCAAACCAGGTTCAAACGCAGACCAAATGTACTGGAAACACAAAGCCATGGAAGTCAAGGCCCAAGTCGTCGAAGGAAAAGGACCCATTTACGCCCGAGTAGAGCCGGACGGGTTCATTGGAATGTACCAAACCGGGAACCCAGCTCCGGTCGACATCCAACCGTTCATGACCTATCCGAGGCTAATCAACGGCCTCCGTAAAGTCCGTTTGGAACCGGACGGAAACCTCAAGGCGTATTACTGGGGCGGTTCGAACTGGGCTTTGGATTTCCAGGCGATTGACACCACGTGCGAGCTTCCCAGTCCATGCGGTTCGTTCGGTTTGTGCGAGGCCGGGAACAGTTCGTGTTCGTGTTTGGATAACCAGACGGAGTTCCGGTCCGGCGAGTGTTTTCCGGTTCAGAGCGGCGACTTCTGCGGTTCCGGATGGGGGGAAATTAATAGCTTCTGGGTTTTAAGGAGGAGCGGCGTGGAGCTGCCGTACAAGGAGTTGATGCGGTATAAAACGATGTCGTCTTATGGGGAATGCGAACGAACCTGTGAAAGCAACTGTAGCTGTTGGGGCGCGGTGTACAACAACGGGTCCGGGTTTTGTTACTTGATTGATTACCCGATCCAGAGTTTGGTGGGCGTTGGGGATGAGAGTAAGATGGGTTATTTTAAGGTGCGGGAGGGTGCAGGAAGGAAGAAAATAAATGTGGGTGTGGGAGTTGGGATTGGGGTGGTTTGTGTGGCACTTTTGATATTTATTGGGGTGGTGGGGGTTTGGAGGTTTAGGGTTTGGAGGAGGAAGAGAGGAGGAGCAGGGAAGAGGTTCATGGGTCAAGATGGCGGTGTTTCCCCCGGCCCGTATAAGGATCTCGGGTCCGCAAGCTTTAAGTCCATTGAGATTGTCGGGCAAAATAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0012505 GO:0016020 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_1 PAN_2 S_locus_glycop

Protein Analysis

536

Amino Acids

58.92

Weight (kDa)

8.04

Isoelectric Point (pI)

38.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	127 - 215	1e-10	D-mannose binding lectin
S_locus_glycop	PF00954	297 - 353	1.2e-06	S-locus glycoprotein domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013446)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03700
fragaria_vesca	FvH4_6g17930
malus_domestica	MD12G1018400.v1.1 MD14G1016400.v1.1
prunus_persica	Prupe.7G116800_v2.0.a1
pyrus_communis	pycom12g01520 pycom14g01410
rosa_chinensis	RchiOBHm_Chr3g0472201
rosa_laevigata	RLG00000024098
rosa_multiflora	Rmu_sc0002532.1_g000034
rosa_roxburghii	Rroxscaffold_6G00409160
rosa_rugosa	Rorug03G0122800
rosa_samantha	Rh3AG175100 Rh3BG200100 Rh3CG191600 Rh3CG198900 Rh3DG174300
rosa_wichuraiana	Rw3G016620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1321
AccBSI	CCGCTC	3 cut(s)	245, 1104, 1164
AccI	GTMKAC	1 cut(s)	849
AccII	CGCG	2 cut(s)	692, 1265
AccIII	TCCGGA	2 cut(s)	247, 1121
AclI	AACGTT	2 cut(s)	113, 558
AclWI	GGATC	2 cut(s)	1306, 1563
AcsI	RAATTY	1 cut(s)	610
AcvI	CACGTG	2 cut(s)	358, 987
AdeI	CACNNNGTG	1 cut(s)	987
AfaI	GTAC	5 cut(s)	689, 728, 824, 1181, 1271
AfiI	CCNNNNNNNGG	7 cut(s)	33, 504, 844, 876, 1128, 1321, 1552
AgsI	TTSAA	5 cut(s)	238, 258, 436, 700, 711
AjnI	CCWGG	2 cut(s)	703, 969
AjuI	GAANNNNNNNTTGG	4 cut(s)	207, 239, 944, 976
Alw21I	GWGCWC	1 cut(s)	143
Alw26I	GTCTC	1 cut(s)	640
AlwI	GGATC	2 cut(s)	1306, 1563
Ama87I	CYCGRG	2 cut(s)	792, 1559
Aor13HI	TCCGGA	2 cut(s)	247, 1121
AoxI	GGCC	5 cut(s)	646, 756, 889, 1029, 1543
ApeKI	GCWGC	6 cut(s)	6, 9, 12, 37, 441, 1174
ApoI	RAATTY	1 cut(s)	610
AseI	ATTAAT	1 cut(s)	1137
Asp700I	GAANNNNTTC	1 cut(s)	1511
AspLEI	GCGC	1 cut(s)	1265
AspS9I	GGNCC	8 cut(s)	250, 411, 757, 779, 1077, 1281, 1544, 1563
AsuC2I	CCSGG	4 cut(s)	832, 1033, 1285, 1542
AsuHPI	GGTGA	1 cut(s)	274
AsuII	TTCGAA	1 cut(s)	950
AvaI	CYCGRG	2 cut(s)	792, 1559
AvaII	GGWCC	6 cut(s)	250, 411, 779, 1077, 1281, 1563
BanII	GRGCYC	1 cut(s)	143
BbrPI	CACGTG	2 cut(s)	358, 987
Bbv12I	GWGCWC	1 cut(s)	143
BbvI	GCAGC	3 cut(s)	24, 453, 1161
BccI	CCATC	3 cut(s)	1119, 1342, 1520
BceAI	ACGGC	4 cut(s)	454, 661, 904, 1162
BcgI	CGANNNNNNTGC	2 cut(s)	491, 525
BciT130I	CCWGG	2 cut(s)	705, 971
BcnI	CCSGG	4 cut(s)	832, 1033, 1285, 1542
BcoDI	GTCTC	1 cut(s)	640
BfaI	CTAG	1 cut(s)	338
BfmI	CTRYAG	2 cut(s)	49, 1249
BlpI	GCTNAGC	2 cut(s)	142, 147
Bme1390I	CCNGG	6 cut(s)	705, 832, 971, 1033, 1285, 1542
Bme18I	GGWCC	6 cut(s)	250, 411, 779, 1077, 1281, 1563
BmeT110I	CYCGRG	2 cut(s)	792, 1559
BmgT120I	GGNCC	8 cut(s)	250, 411, 757, 779, 1077, 1281, 1544, 1563
BmiI	GGNNCC	7 cut(s)	252, 781, 836, 912, 1120, 1282, 1564
BmrFI	CCNGG	6 cut(s)	705, 832, 971, 1033, 1285, 1542
BmrI	ACTGGG	5 cut(s)	317, 457, 949, 964, 993
BmsI	GCATC	2 cut(s)	648, 1182
BmuI	ACTGGG	5 cut(s)	317, 457, 949, 964, 993
BplI	GAGNNNNNCTC	2 cut(s)	130, 162
Bpu1102I	GCTNAGC	2 cut(s)	142, 147
Bpu14I	TTCGAA	1 cut(s)	950
BpuEI	CTTGAG	1 cut(s)	911
BpuMI	CCSGG	4 cut(s)	832, 1033, 1285, 1542
BsaAI	YACGTR	2 cut(s)	358, 987
BsaJI	CCNNGG	4 cut(s)	32, 744, 875, 1540
BsaWI	WCCGGW	6 cut(s)	247, 844, 913, 1074, 1093, 1121
BsaXI	ACNNNNNCTCC	4 cut(s)	1061, 1091, 1163, 1193
Bsc4I	CCNNNNNNNGG	7 cut(s)	33, 504, 844, 876, 1128, 1321, 1552
Bse118I	RCCGGY	2 cut(s)	293, 497
Bse1I	ACTGG	6 cut(s)	312, 463, 734, 944, 959, 999
BseAI	TCCGGA	2 cut(s)	247, 1121
BseBI	CCWGG	2 cut(s)	705, 971
BseDI	CCNNGG	4 cut(s)	32, 744, 875, 1540
BseGI	GGATG	4 cut(s)	638, 852, 1130, 1342
BseLI	CCNNNNNNNGG	7 cut(s)	33, 504, 844, 876, 1128, 1321, 1552
BseMII	CTCAG	4 cut(s)	128, 156, 161, 563
BseNI	ACTGG	6 cut(s)	312, 463, 734, 944, 959, 999
BseRI	GAGGAG	6 cut(s)	341, 419, 1174, 1498, 1509, 1512
BseXI	GCAGC	3 cut(s)	24, 453, 1161
BseYI	CCCAGC	1 cut(s)	838
BsgI	GTGCAG	1 cut(s)	1395
Bsh1236I	CGCG	2 cut(s)	692, 1265
Bsh1285I	CGRYCG	3 cut(s)	418, 505, 849
BshFI	GGCC	5 cut(s)	648, 758, 891, 1031, 1545
BsiEI	CGRYCG	3 cut(s)	418, 505, 849
BsiHKAI	GWGCWC	1 cut(s)	143
BsiHKCI	CYCGRG	2 cut(s)	792, 1559
BslI	CCNNNNNNNGG	7 cut(s)	33, 504, 844, 876, 1128, 1321, 1552
BsmAI	GTCTC	1 cut(s)	640
BsmI	GAATGC	1 cut(s)	1229
BsnI	GGCC	5 cut(s)	648, 758, 891, 1031, 1545
BsoBI	CYCGRG	2 cut(s)	792, 1559
Bsp119I	TTCGAA	1 cut(s)	950
Bsp1286I	GDGCHC	1 cut(s)	143
Bsp13I	TCCGGA	2 cut(s)	247, 1121
Bsp1407I	TGTACA	1 cut(s)	1269
Bsp143I	GATC	3 cut(s)	502, 1311, 1555
Bsp1720I	GCTNAGC	2 cut(s)	142, 147
Bsp19I	CCATGG	2 cut(s)	32, 744
BspANI	GGCC	5 cut(s)	648, 758, 891, 1031, 1545
BspCNI	CTCAG	4 cut(s)	129, 155, 160, 562
BspEI	TCCGGA	2 cut(s)	247, 1121
BspFNI	CGCG	2 cut(s)	692, 1265
BspHI	TCATGA	1 cut(s)	864
BspLI	GGNNCC	7 cut(s)	252, 781, 836, 912, 1120, 1282, 1564
BspPI	GGATC	2 cut(s)	1306, 1563
BspT104I	TTCGAA	1 cut(s)	950
BsrBI	CCGCTC	3 cut(s)	245, 1104, 1164
BsrFI	RCCGGY	2 cut(s)	293, 497
BsrGI	TGTACA	1 cut(s)	1269
BsrI	ACTGG	6 cut(s)	312, 463, 734, 944, 959, 999
BssAI	RCCGGY	2 cut(s)	293, 497
BssECI	CCNNGG	4 cut(s)	32, 744, 875, 1540
BssMI	GATC	3 cut(s)	502, 1311, 1555
BssT1I	CCWWGG	2 cut(s)	32, 744
Bst2UI	CCWGG	2 cut(s)	705, 971
Bst4CI	ACNGT	4 cut(s)	172, 861, 1040, 1250
Bst6I	CTCTTC	3 cut(s)	437, 1484, 1502
BstAUI	TGTACA	1 cut(s)	1269
BstBAI	YACGTR	2 cut(s)	358, 987
BstBI	TTCGAA	1 cut(s)	950
BstC8I	GCNNGC	4 cut(s)	384, 406, 992, 1570
BstDEI	CTNAG	5 cut(s)	137, 142, 147, 454, 549
BstDSI	CCRYGG	2 cut(s)	32, 744
BstENI	CCTNNNNNAGG	1 cut(s)	874
BstF5I	GGATG	4 cut(s)	638, 852, 1130, 1342
BstFNI	CGCG	2 cut(s)	692, 1265
BstHHI	GCGC	1 cut(s)	1265
BstKTI	GATC	3 cut(s)	505, 1314, 1558
BstMAI	GTCTC	1 cut(s)	640
BstMBI	GATC	3 cut(s)	502, 1311, 1555
BstMCI	CGRYCG	3 cut(s)	418, 505, 849
BstMWI	GCNNNNNNNGC	5 cut(s)	12, 215, 1113, 1251, 1260
BstNI	CCWGG	2 cut(s)	705, 971
BstSCI	CCNGG	6 cut(s)	703, 830, 969, 1031, 1283, 1540
BstSFI	CTRYAG	2 cut(s)	49, 1249
BstUI	CGCG	2 cut(s)	692, 1265
BstV1I	GCAGC	3 cut(s)	24, 453, 1161
BstX2I	RGATCY	1 cut(s)	1555
BstYI	RGATCY	1 cut(s)	1555
BsuRI	GGCC	5 cut(s)	648, 758, 891, 1031, 1545
BtgI	CCRYGG	2 cut(s)	32, 744
BtgZI	GCGATG	1 cut(s)	174
BtsCI	GGATG	4 cut(s)	638, 852, 1130, 1342
BtsIMutI	CAGTG	3 cut(s)	132, 168, 305
Cac8I	GCNNGC	4 cut(s)	384, 406, 992, 1570
CciI	TCATGA	1 cut(s)	864
CfoI	GCGC	1 cut(s)	1265
Cfr10I	RCCGGY	2 cut(s)	293, 497
Cfr13I	GGNCC	8 cut(s)	250, 411, 757, 779, 1077, 1281, 1544, 1563
CpoI	CGGWCCG	1 cut(s)	1077
CsiI	ACCWGGT	1 cut(s)	703
Csp6I	GTAC	5 cut(s)	688, 727, 823, 1180, 1270
CspI	CGGWCCG	1 cut(s)	1077
CviAII	CATG	7 cut(s)	33, 625, 679, 745, 865, 1005, 1516
CviQI	GTAC	5 cut(s)	688, 727, 823, 1180, 1270
DdeI	CTNAG	5 cut(s)	137, 142, 147, 454, 549
DpnI	GATC	3 cut(s)	504, 1313, 1557
DpnII	GATC	3 cut(s)	502, 1311, 1555
DraIII	CACNNNGTG	1 cut(s)	987
Eam1104I	CTCTTC	3 cut(s)	437, 1484, 1502
EarI	CTCTTC	3 cut(s)	437, 1484, 1502
Ecl136II	GAGCTC	1 cut(s)	141
Eco130I	CCWWGG	2 cut(s)	32, 744
Eco24I	GRGCYC	1 cut(s)	143
Eco47I	GGWCC	6 cut(s)	250, 411, 779, 1077, 1281, 1563
Eco53kI	GAGCTC	1 cut(s)	141
Eco72I	CACGTG	2 cut(s)	358, 987
Eco88I	CYCGRG	2 cut(s)	792, 1559
EcoICRI	GAGCTC	1 cut(s)	141
EcoNI	CCTNNNNNAGG	1 cut(s)	874
EcoO109I	RGGNCCY	1 cut(s)	779
EcoRII	CCWGG	2 cut(s)	703, 969
EcoT14I	CCWWGG	2 cut(s)	32, 744
EcoT38I	GRGCYC	1 cut(s)	143
ErhI	CCWWGG	2 cut(s)	32, 744
FaeI	CATG	7 cut(s)	36, 628, 682, 748, 868, 1008, 1519
FalI	AAGNNNNNCTT	2 cut(s)	207, 239
FatI	CATG	7 cut(s)	32, 624, 678, 744, 864, 1004, 1515
FauI	CCCGC	2 cut(s)	413, 1359
FblI	GTMKAC	1 cut(s)	849
FokI	GGATG	4 cut(s)	625, 839, 1137, 1349
FriOI	GRGCYC	1 cut(s)	143
FspBI	CTAG	1 cut(s)	338
GlaI	GCGC	1 cut(s)	1264
GsaI	CCCAGC	1 cut(s)	842
HaeIII	GGCC	5 cut(s)	648, 758, 891, 1031, 1545
HhaI	GCGC	1 cut(s)	1265
Hin1II	CATG	7 cut(s)	36, 628, 682, 748, 868, 1008, 1519
Hin6I	GCGC	1 cut(s)	1263
HinP1I	GCGC	1 cut(s)	1263
HincII	GTYRAC	3 cut(s)	325, 483, 850
HindII	GTYRAC	3 cut(s)	325, 483, 850
HindIII	AAGCTT	2 cut(s)	629, 1570
HinfI	GANTC	3 cut(s)	130, 321, 458
HphI	GGTGA	1 cut(s)	274
Hpy166II	GTNNAC	5 cut(s)	117, 325, 483, 850, 1270
Hpy188I	TCNGA	7 cut(s)	90, 138, 320, 370, 415, 876, 1101
Hpy188III	TCNNGA	8 cut(s)	248, 468, 551, 601, 865, 1122, 1315, 1523
Hpy8I	GTNNAC	5 cut(s)	117, 325, 483, 850, 1270
Hpy99I	CGWCG	3 cut(s)	206, 602, 770
HpyAV	CCTTC	5 cut(s)	264, 277, 372, 764, 1374
HpyCH4III	ACNGT	4 cut(s)	172, 861, 1040, 1250
HpyCH4IV	ACGT	8 cut(s)	113, 183, 201, 357, 531, 558, 579, 986
HpyCH4V	TGCA	3 cut(s)	512, 661, 1376
HpyF10VI	GCNNNNNNNGC	5 cut(s)	12, 215, 1113, 1251, 1260
HpyF3I	CTNAG	5 cut(s)	137, 142, 147, 454, 549
HpySE526I	ACGT	8 cut(s)	113, 183, 201, 357, 531, 558, 579, 986
Hsp92II	CATG	7 cut(s)	36, 628, 682, 748, 868, 1008, 1519
HspAI	GCGC	1 cut(s)	1263
Kpn2I	TCCGGA	2 cut(s)	247, 1121
Kzo9I	GATC	3 cut(s)	502, 1311, 1555
LmnI	GCTCC	6 cut(s)	220, 250, 847, 1161, 1171, 1499
Lsp1109I	GCAGC	3 cut(s)	24, 453, 1161
LweI	GCATC	2 cut(s)	648, 1182
MabI	ACCWGGT	1 cut(s)	703
MaeI	CTAG	1 cut(s)	338
MaeII	ACGT	8 cut(s)	113, 183, 201, 357, 531, 558, 579, 986
MaeIII	GTNAC	3 cut(s)	127, 184, 1292
MalI	GATC	3 cut(s)	504, 1313, 1557
MbiI	CCGCTC	3 cut(s)	245, 1104, 1164
MboI	GATC	3 cut(s)	502, 1311, 1555
MboII	GAAGA	5 cut(s)	146, 424, 1396, 1501, 1519
MfeI	CAATTG	1 cut(s)	662
MflI	RGATCY	1 cut(s)	1555
MhlI	GDGCHC	1 cut(s)	143
MluCI	AATT	3 cut(s)	610, 662, 1134
MlyI	GAGTC	2 cut(s)	124, 330
MmeI	TCCRAC	6 cut(s)	295, 393, 438, 552, 666, 880
MroI	TCCGGA	2 cut(s)	247, 1121
MroXI	GAANNNNTTC	1 cut(s)	1511
MseI	TTAA	5 cut(s)	1137, 1154, 1359, 1575, 1606
MslI	CAYNNNNRTG	1 cut(s)	818
MspA1I	CMGCKG	1 cut(s)	408
MspR9I	CCNGG	6 cut(s)	705, 832, 971, 1033, 1285, 1542
MunI	CAATTG	1 cut(s)	662
Mva1269I	GAATGC	1 cut(s)	1229
MvaI	CCWGG	2 cut(s)	705, 971
MvnI	CGCG	2 cut(s)	692, 1265
MwoI	GCNNNNNNNGC	5 cut(s)	12, 215, 1113, 1251, 1260
NciI	CCSGG	4 cut(s)	832, 1033, 1285, 1542
NcoI	CCATGG	2 cut(s)	32, 744
NdeII	GATC	3 cut(s)	502, 1311, 1555
NlaIII	CATG	7 cut(s)	36, 628, 682, 748, 868, 1008, 1519
NlaIV	GGNNCC	7 cut(s)	252, 781, 836, 912, 1120, 1282, 1564
NmuCI	GTSAC	2 cut(s)	127, 184
NspV	TTCGAA	1 cut(s)	950
PagI	TCATGA	1 cut(s)	864
PcsI	WCGNNNNNNNCGW	2 cut(s)	444, 1023
PctI	GAATGC	1 cut(s)	1229
PdmI	GAANNNNTTC	1 cut(s)	1511
PfeI	GAWTC	1 cut(s)	458
PflMI	CCANNNNNTGG	1 cut(s)	1321
Ple19I	CGATCG	1 cut(s)	505
PleI	GAGTC	2 cut(s)	124, 329
PmaCI	CACGTG	2 cut(s)	358, 987
PmlI	CACGTG	2 cut(s)	358, 987
PpsI	GAGTC	2 cut(s)	124, 329
Ppu21I	YACGTR	2 cut(s)	358, 987
PpuMI	RGGWCCY	1 cut(s)	779
PshBI	ATTAAT	1 cut(s)	1137
Psp124BI	GAGCTC	1 cut(s)	143
Psp1406I	AACGTT	2 cut(s)	113, 558
Psp5II	RGGWCCY	1 cut(s)	779
Psp6I	CCWGG	2 cut(s)	703, 969
PspCI	CACGTG	2 cut(s)	358, 987
PspFI	CCCAGC	1 cut(s)	838
PspGI	CCWGG	2 cut(s)	703, 969
PspN4I	GGNNCC	7 cut(s)	252, 781, 836, 912, 1120, 1282, 1564
PspPI	GGNCC	8 cut(s)	250, 411, 757, 779, 1077, 1281, 1544, 1563
PspPPI	RGGWCCY	1 cut(s)	779
PsrI	GAACNNNNNNTAC	2 cut(s)	680, 712
PsuI	RGATCY	1 cut(s)	1555
PvuI	CGATCG	1 cut(s)	505
RsaI	GTAC	5 cut(s)	689, 728, 824, 1181, 1271
RsaNI	GTAC	5 cut(s)	688, 727, 823, 1180, 1270
RseI	CAYNNNNRTG	1 cut(s)	818
Rsr2I	CGGWCCG	1 cut(s)	1077
RsrII	CGGWCCG	1 cut(s)	1077
SacI	GAGCTC	1 cut(s)	143
SalI	GTCGAC	1 cut(s)	848
SaqAI	TTAA	5 cut(s)	1137, 1154, 1359, 1575, 1606
Sau3AI	GATC	3 cut(s)	502, 1311, 1555
Sau96I	GGNCC	8 cut(s)	250, 411, 757, 779, 1077, 1281, 1544, 1563
SchI	GAGTC	2 cut(s)	124, 330
ScrFI	CCNGG	6 cut(s)	705, 832, 971, 1033, 1285, 1542
SduI	GDGCHC	1 cut(s)	143
SexAI	ACCWGGT	1 cut(s)	703
SfaNI	GCATC	2 cut(s)	648, 1182
SfcI	CTRYAG	2 cut(s)	49, 1249
SfuI	TTCGAA	1 cut(s)	950
SinI	GGWCC	6 cut(s)	250, 411, 779, 1077, 1281, 1563
SmiMI	CAYNNNNRTG	1 cut(s)	818
SmlI	CTYRAG	1 cut(s)	926
SmoI	CTYRAG	1 cut(s)	926
Sse9I	AATT	3 cut(s)	610, 662, 1134
SspMI	CTAG	1 cut(s)	338
SstI	GAGCTC	1 cut(s)	143
StyD4I	CCNGG	6 cut(s)	703, 830, 969, 1031, 1283, 1540
StyI	CCWWGG	2 cut(s)	32, 744
TaaI	ACNGT	4 cut(s)	172, 861, 1040, 1250
TaiI	ACGT	8 cut(s)	116, 186, 204, 360, 534, 561, 582, 989
TaqI	TCGA	8 cut(s)	467, 519, 600, 620, 656, 768, 849, 950
TasI	AATT	3 cut(s)	610, 662, 1134
TatI	WGTACW	2 cut(s)	726, 1269
TauI	GCSGC	5 cut(s)	209, 212, 245, 1107, 1167
TfiI	GAWTC	1 cut(s)	458
Tru1I	TTAA	5 cut(s)	1137, 1154, 1359, 1575, 1606
Tru9I	TTAA	5 cut(s)	1137, 1154, 1359, 1575, 1606
TscAI	CASTG	3 cut(s)	132, 175, 312
TseFI	GTSAC	2 cut(s)	127, 184
TseI	GCWGC	6 cut(s)	6, 9, 12, 37, 441, 1174
Tsp45I	GTSAC	2 cut(s)	127, 184
TspDTI	ATGAA	6 cut(s)	85, 641, 667, 802, 853, 1504
TspGWI	ACGGA	4 cut(s)	884, 893, 933, 1082
TspRI	CASTG	3 cut(s)	132, 175, 312
Van91I	CCANNNNNTGG	1 cut(s)	1321
VpaK11BI	GGWCC	6 cut(s)	250, 411, 779, 1077, 1281, 1563
VspI	ATTAAT	1 cut(s)	1137
XagI	CCTNNNNNAGG	1 cut(s)	874
XapI	RAATTY	1 cut(s)	610
XmiI	GTMKAC	1 cut(s)	849
XmnI	GAANNNNTTC	1 cut(s)	1511
XspI	CTAG	1 cut(s)	338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.