MD14G1020400.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013245
Physical Location & Seq
Forward (+)
1915448 .. 1917990
2543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1020400.v1.1.491

Sequence Viewer

Length: 999 bp
ATGGCGGACGTCCAACGCCGGCCGGGACTGGTTCTCTTTGTTTTTACGTTAATGGGGTTGGCTTTCCCGGTGTTGTTTGGTGCATTTGTCCAGGGGTATAATCGTCCACCGTCTCGCAAGAACCTCTACGTCCCTGCGGATGATCCTGGTTCTGCTTCTCCACAACAGGTGCATATTTCAGTAGTAGGCGAGGACAAAATGAGGGTGACATGGATCACAGACATCCCTAGTCCGGCAACAGTGGTATATGGAACATCTCCAGGTGCTTATGAAAGCACTGCAACTGGAAATACAAGTTCCTACACTCATGGAATAATATACACGTCTGGGGATATTCAGGACGTCGTTATCGGTTCGTTGAAACCCAACACGCAGTACTACTGCCGCTGTGGCTCCTCTCATTCGGATCCTGAGTTCAGTTTCAAAACCCCGCCTGCAGCATTCCCTATCACATTTGCAGTCGTAGGTGATCTTGGACAAACTGAATGGACCTCCTCAACCCTCCAACACATAGCCAAAACAAACCACGACATGTTATTACTACCAGGAGACTTATCCTATGCAGACTTGCGCCAAAGTAAATGGAACTCATTTGGCCCTCTGGTGCAGCCACTGGCAAGCCAACGGCCTTGGATGGTCACACAAGGCAATCACGAGGTCGAAAAGATCCCGATCTTCCACCCGCATGCCTTCACCGCATACAATGCGCGTATCAGTGGGTTGCAAGCGAATTTGGGAAAGGTCGACAGGGGGAGGACGCCGTGGATCGTCGTGCTTATCCATGCTCCTTGGTACAATTCTAATAAAGCTCATCAGGGTGAGTCTGAATCTGTTGACATGAAGGAGGCCATGGAAGATTTGCTCTATCAAGCTAGTGTTGATGTGGTTTATGCAGGGCACGTACATGCCTACGAGCGTTTTGATCAAGCCAATAATTGTAGTCCAGTGCATATTACCATTGGAGATGGTGGCAACCGTGAAGGCCTTGCTAGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

333

Amino Acids

36.41

Weight (kDa)

6.09

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 54 - 143 1.7e-18 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 151 - 304 7.9e-13 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013245)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52780 AT3G52780
fragaria_vesca FvH4_6g18430 FvH4_6g18430
malus_domestica MD12G1023400.v1.1 MD14G1020400.v1.1
prunus_persica Prupe.7G113100_v2.0.a1
pyrus_communis pycom12g02180
rosa_chinensis RchiOBHm_Chr3g0472961
rosa_laevigata RLG00000024044
rosa_multiflora Rmu_sc0021557.1_g000005
rosa_roxburghii Rroxscaffold_6G00408460
rosa_rugosa Rorug03G0129900
rosa_samantha Rh3AG182000 Rh3BG208300 Rh3CG205300 Rh3DG167800
rosa_wichuraiana Rw3G016150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 12, 345
AccI GTMKAC 1 cut(s) 744
AccII CGCG 1 cut(s) 709
AciI CCGC 6 cut(s) 5, 137, 385, 431, 683, 696
AclWI GGATC 6 cut(s) 137, 221, 401, 414, 661, 773
AcoI YGGCCR 1 cut(s) 20
AcsI RAATTY 1 cut(s) 730
AcyI GRCGYC 3 cut(s) 9, 342, 758
AfaI GTAC 3 cut(s) 377, 794, 903
AfiI CCNNNNNNNGG 1 cut(s) 232
AflIII ACRYGT 2 cut(s) 321, 531
AgsI TTSAA 2 cut(s) 361, 424
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 4 cut(s) 90, 145, 259, 544
AloI GAACNNNNNNTCC 2 cut(s) 398, 430
AluBI AGCT 2 cut(s) 809, 872
AluI AGCT 2 cut(s) 809, 872
Alw26I GTCTC 2 cut(s) 117, 543
AlwI GGATC 6 cut(s) 137, 221, 401, 414, 661, 773
AlwNI CAGNNNCTG 1 cut(s) 613
AoxI GGCC 5 cut(s) 20, 595, 626, 846, 982
ApeKI GCWGC 2 cut(s) 437, 607
ApoI RAATTY 1 cut(s) 730
AspLEI GCGC 2 cut(s) 573, 709
AspS9I GGNCC 2 cut(s) 489, 596
AsuC2I CCSGG 2 cut(s) 24, 68
AsuHPI GGTGA 4 cut(s) 217, 479, 685, 830
AsuNHI GCTAGC 1 cut(s) 989
AvaII GGWCC 1 cut(s) 489
BaeGI GKGCMC 1 cut(s) 900
BamHI GGATCC 1 cut(s) 406
BauI CACGAG 1 cut(s) 653
BbvI GCAGC 2 cut(s) 449, 619
BccI CCATC 2 cut(s) 628, 959
BceAI ACGGC 2 cut(s) 641, 745
BciT130I CCWGG 4 cut(s) 92, 147, 261, 546
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 2 cut(s) 24, 68
BcoDI GTCTC 2 cut(s) 117, 543
BfaI CTAG 3 cut(s) 228, 873, 990
BfmI CTRYAG 1 cut(s) 435
BglI GCCNNNNNGGC 1 cut(s) 390
BisI GCNGC 3 cut(s) 385, 438, 608
BlsI GCNGC 3 cut(s) 386, 439, 609
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 6 cut(s) 24, 68, 92, 147, 261, 546
Bme18I GGWCC 1 cut(s) 489
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 2 cut(s) 489, 596
BmiI GGNNCC 2 cut(s) 394, 408
BmrFI CCNGG 6 cut(s) 24, 68, 92, 147, 261, 546
BmtI GCTAGC 1 cut(s) 993
BpmI CTGGAG 1 cut(s) 243
BpuMI CCSGG 2 cut(s) 24, 68
BsaAI YACGTR 1 cut(s) 901
BsaBI GATNNNNATC 1 cut(s) 671
BsaHI GRCGYC 3 cut(s) 9, 342, 758
BsaJI CCNNGG 5 cut(s) 91, 629, 761, 788, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 4 cut(s) 33, 289, 618, 944
Bse8I GATNNNNATC 1 cut(s) 671
BseBI CCWGG 4 cut(s) 92, 147, 261, 546
BseDI CCNNGG 5 cut(s) 91, 629, 761, 788, 849
BseGI GGATG 3 cut(s) 145, 222, 639
BseJI GATNNNNATC 1 cut(s) 671
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 4 cut(s) 33, 289, 618, 944
BseRI GAGGAG 2 cut(s) 385, 484
BseSI GKGCMC 1 cut(s) 900
BseX3I CGGCCG 1 cut(s) 20
BseXI GCAGC 2 cut(s) 449, 619
BsgI GTGCAG 1 cut(s) 626
Bsh1236I CGCG 1 cut(s) 709
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 5 cut(s) 22, 597, 628, 848, 984
BsiEI CGRYCG 1 cut(s) 23
BsiSI CCGG 4 cut(s) 19, 23, 68, 233
BslFI GGGAC 2 cut(s) 39, 116
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 2 cut(s) 117, 543
BsmBI CGTCTC 1 cut(s) 117
BsmFI GGGAC 2 cut(s) 39, 116
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 5 cut(s) 22, 597, 628, 848, 984
Bsp1286I GDGCHC 1 cut(s) 900
Bsp143I GATC 8 cut(s) 142, 213, 406, 469, 666, 672, 765, 922
Bsp19I CCATGG 1 cut(s) 849
BspACI CCGC 6 cut(s) 5, 137, 385, 431, 683, 696
BspANI GGCC 5 cut(s) 22, 597, 628, 848, 984
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 1 cut(s) 709
BspLI GGNNCC 2 cut(s) 394, 408
BspMAI CTGCAG 1 cut(s) 439
BspOI GCTAGC 1 cut(s) 993
BspPI GGATC 6 cut(s) 137, 221, 401, 414, 661, 773
BsrFI RCCGGY 1 cut(s) 18
BsrI ACTGG 4 cut(s) 33, 289, 618, 944
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 5 cut(s) 91, 629, 761, 788, 849
BssMI GATC 8 cut(s) 142, 213, 406, 469, 666, 672, 765, 922
BssNI GRCGYC 3 cut(s) 9, 342, 758
BssSI CACGAG 1 cut(s) 653
BssT1I CCWWGG 3 cut(s) 629, 788, 849
Bst2BI CACGAG 1 cut(s) 653
Bst2UI CCWGG 4 cut(s) 92, 147, 261, 546
Bst4CI ACNGT 3 cut(s) 111, 241, 977
BstACI GRCGYC 3 cut(s) 9, 342, 758
BstAPI GCANNNNNTGC 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 901
BstC8I GCNNGC 6 cut(s) 20, 435, 619, 687, 726, 991
BstDEI CTNAG 1 cut(s) 411
BstDSI CCRYGG 2 cut(s) 761, 849
BstF5I GGATG 3 cut(s) 145, 222, 639
BstFNI CGCG 1 cut(s) 709
BstHHI GCGC 2 cut(s) 573, 709
BstKTI GATC 8 cut(s) 145, 216, 409, 472, 669, 675, 768, 925
BstMAI GTCTC 2 cut(s) 117, 543
BstMBI GATC 8 cut(s) 142, 213, 406, 469, 666, 672, 765, 922
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 4 cut(s) 390, 695, 704, 990
BstNI CCWGG 4 cut(s) 92, 147, 261, 546
BstNSI RCATGY 3 cut(s) 535, 689, 908
BstSCI CCNGG 6 cut(s) 22, 66, 90, 145, 259, 544
BstSFI CTRYAG 1 cut(s) 435
BstSLI GKGCMC 1 cut(s) 900
BstUI CGCG 1 cut(s) 709
BstV1I GCAGC 2 cut(s) 449, 619
BstX2I RGATCY 2 cut(s) 406, 666
BstYI RGATCY 2 cut(s) 406, 666
BstZI CGGCCG 1 cut(s) 20
BsuRI GGCC 5 cut(s) 22, 597, 628, 848, 984
BtgI CCRYGG 2 cut(s) 761, 849
BtrI CACGTC 1 cut(s) 324
BtsCI GGATG 3 cut(s) 145, 222, 639
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 5 cut(s) 246, 276, 611, 721, 951
Cac8I GCNNGC 6 cut(s) 20, 435, 619, 687, 726, 991
CaiI CAGNNNCTG 1 cut(s) 613
CfoI GCGC 2 cut(s) 573, 709
Cfr10I RCCGGY 1 cut(s) 18
Cfr13I GGNCC 2 cut(s) 489, 596
CseI GACGC 1 cut(s) 766
Csp6I GTAC 3 cut(s) 376, 793, 902
CviAII CATG 8 cut(s) 210, 308, 532, 686, 782, 838, 850, 905
CviQI GTAC 3 cut(s) 376, 793, 902
DdeI CTNAG 1 cut(s) 411
DpnI GATC 8 cut(s) 144, 215, 408, 471, 668, 674, 767, 924
DpnII GATC 8 cut(s) 142, 213, 406, 469, 666, 672, 765, 922
EaeI YGGCCR 1 cut(s) 20
EagI CGGCCG 1 cut(s) 20
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 629, 788, 849
Eco147I AGGCCT 1 cut(s) 984
Eco47I GGWCC 1 cut(s) 489
Eco52I CGGCCG 1 cut(s) 20
EcoRII CCWGG 4 cut(s) 90, 145, 259, 544
EcoT14I CCWWGG 3 cut(s) 629, 788, 849
ErhI CCWWGG 3 cut(s) 629, 788, 849
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 8 cut(s) 213, 311, 535, 689, 785, 841, 853, 908
FaqI GGGAC 2 cut(s) 39, 116
FatI CATG 8 cut(s) 209, 307, 531, 685, 781, 837, 849, 904
FauI CCCGC 2 cut(s) 438, 690
FbaI TGATCA 1 cut(s) 922
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 3 cut(s) 385, 438, 608
FokI GGATG 3 cut(s) 152, 209, 646
Fsp4HI GCNGC 3 cut(s) 385, 438, 608
FspBI CTAG 3 cut(s) 228, 873, 990
GlaI GCGC 2 cut(s) 572, 708
GluI GCNGC 3 cut(s) 385, 438, 608
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 5 cut(s) 22, 597, 628, 848, 984
HapII CCGG 4 cut(s) 19, 23, 68, 233
HgaI GACGC 1 cut(s) 766
HhaI GCGC 2 cut(s) 573, 709
Hin1I GRCGYC 3 cut(s) 9, 342, 758
Hin1II CATG 8 cut(s) 213, 311, 535, 689, 785, 841, 853, 908
Hin6I GCGC 2 cut(s) 571, 707
HinP1I GCGC 2 cut(s) 571, 707
HincII GTYRAC 2 cut(s) 745, 835
HindII GTYRAC 2 cut(s) 745, 835
HinfI GANTC 2 cut(s) 821, 827
HpaII CCGG 4 cut(s) 19, 23, 68, 233
HphI GGTGA 4 cut(s) 217, 479, 685, 830
Hpy166II GTNNAC 3 cut(s) 107, 745, 835
Hpy188I TCNGA 2 cut(s) 406, 826
Hpy188III TCNNGA 4 cut(s) 338, 410, 653, 670
Hpy8I GTNNAC 3 cut(s) 107, 745, 835
Hpy99I CGWCG 2 cut(s) 347, 773
HpyAV CCTTC 3 cut(s) 700, 835, 974
HpyCH4III ACNGT 3 cut(s) 111, 241, 977
HpyCH4IV ACGT 6 cut(s) 9, 47, 129, 323, 342, 900
HpyF10VI GCNNNNNNNGC 4 cut(s) 390, 695, 704, 990
HpyF3I CTNAG 1 cut(s) 411
HpySE526I ACGT 6 cut(s) 9, 47, 129, 323, 342, 900
Hsp92I GRCGYC 3 cut(s) 9, 342, 758
Hsp92II CATG 8 cut(s) 213, 311, 535, 689, 785, 841, 853, 908
HspAI GCGC 2 cut(s) 571, 707
KroI GCCGGC 1 cut(s) 18
KroNI GCCGGC 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 8 cut(s) 142, 213, 406, 469, 666, 672, 765, 922
LmnI GCTCC 2 cut(s) 398, 790
Lsp1109I GCAGC 2 cut(s) 449, 619
MaeI CTAG 3 cut(s) 228, 873, 990
MaeII ACGT 6 cut(s) 9, 47, 129, 323, 342, 900
MaeIII GTNAC 2 cut(s) 205, 637
MalI GATC 8 cut(s) 144, 215, 408, 471, 668, 674, 767, 924
MboI GATC 8 cut(s) 142, 213, 406, 469, 666, 672, 765, 922
MboII GAAGA 2 cut(s) 667, 866
MflI RGATCY 2 cut(s) 406, 666
MhlI GDGCHC 1 cut(s) 900
MluCI AATT 3 cut(s) 730, 796, 934
MlyI GAGTC 1 cut(s) 830
MmeI TCCRAC 2 cut(s) 37, 529
MroNI GCCGGC 1 cut(s) 18
MseI TTAA 1 cut(s) 50
MslI CAYNNNNRTG 3 cut(s) 684, 816, 903
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 4 cut(s) 19, 23, 68, 233
MspR9I CCNGG 6 cut(s) 24, 68, 92, 147, 261, 546
Mva1269I GAATGC 1 cut(s) 440
MvaI CCWGG 4 cut(s) 92, 147, 261, 546
MvnI CGCG 1 cut(s) 709
MwoI GCNNNNNNNGC 4 cut(s) 390, 695, 704, 990
NaeI GCCGGC 1 cut(s) 20
NciI CCSGG 2 cut(s) 24, 68
NcoI CCATGG 1 cut(s) 849
NdeII GATC 8 cut(s) 142, 213, 406, 469, 666, 672, 765, 922
NgoMIV GCCGGC 1 cut(s) 18
NheI GCTAGC 1 cut(s) 989
NlaIII CATG 8 cut(s) 213, 311, 535, 689, 785, 841, 853, 908
NlaIV GGNNCC 2 cut(s) 394, 408
NmuCI GTSAC 2 cut(s) 205, 637
NspI RCATGY 3 cut(s) 535, 689, 908
PaeI GCATGC 1 cut(s) 689
PceI AGGCCT 1 cut(s) 984
PciI ACATGT 1 cut(s) 531
PctI GAATGC 1 cut(s) 440
PdiI GCCGGC 1 cut(s) 20
PfeI GAWTC 1 cut(s) 827
PkrI GCNGC 3 cut(s) 386, 439, 609
PleI GAGTC 1 cut(s) 829
PpsI GAGTC 1 cut(s) 829
Ppu21I YACGTR 1 cut(s) 901
PscI ACATGT 1 cut(s) 531
Psp6I CCWGG 4 cut(s) 90, 145, 259, 544
PspGI CCWGG 4 cut(s) 90, 145, 259, 544
PspN4I GGNNCC 2 cut(s) 394, 408
PspPI GGNCC 2 cut(s) 489, 596
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 613
PsuI RGATCY 2 cut(s) 406, 666
RsaI GTAC 3 cut(s) 377, 794, 903
RsaNI GTAC 3 cut(s) 376, 793, 902
RseI CAYNNNNRTG 3 cut(s) 684, 816, 903
SalI GTCGAC 1 cut(s) 743
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 3 cut(s) 385, 438, 608
Sau3AI GATC 8 cut(s) 142, 213, 406, 469, 666, 672, 765, 922
Sau96I GGNCC 2 cut(s) 489, 596
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 1 cut(s) 830
ScrFI CCNGG 6 cut(s) 24, 68, 92, 147, 261, 546
SduI GDGCHC 1 cut(s) 900
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 1 cut(s) 489
SmiMI CAYNNNNRTG 3 cut(s) 684, 816, 903
SphI GCATGC 1 cut(s) 689
Sse9I AATT 3 cut(s) 730, 796, 934
SseBI AGGCCT 1 cut(s) 984
SsiI CCGC 6 cut(s) 5, 137, 385, 431, 683, 696
SspMI CTAG 3 cut(s) 228, 873, 990
StuI AGGCCT 1 cut(s) 984
StyD4I CCNGG 6 cut(s) 22, 66, 90, 145, 259, 544
StyI CCWWGG 3 cut(s) 629, 788, 849
TaaI ACNGT 3 cut(s) 111, 241, 977
TaiI ACGT 6 cut(s) 12, 50, 132, 326, 345, 903
TaqI TCGA 2 cut(s) 660, 744
TasI AATT 3 cut(s) 730, 796, 934
TatI WGTACW 1 cut(s) 375
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 1 cut(s) 827
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 5 cut(s) 246, 283, 618, 721, 951
TseFI GTSAC 2 cut(s) 205, 637
TseI GCWGC 2 cut(s) 437, 607
Tsp45I GTSAC 2 cut(s) 205, 637
TspDTI ATGAA 2 cut(s) 285, 854
TspRI CASTG 5 cut(s) 246, 283, 618, 721, 951
VpaK11BI GGWCC 1 cut(s) 489
XapI RAATTY 1 cut(s) 730
XceI RCATGY 3 cut(s) 535, 689, 908
XmiI GTMKAC 1 cut(s) 744
XspI CTAG 3 cut(s) 228, 873, 990
ZraI GACGTC 2 cut(s) 10, 343
ZrmI AGTACT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.