Prupe.7G113100_v2.0.a1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0013245
Physical Location & Seq
Reverse (-)
13903508 .. 13906719
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G113100.1

Sequence Viewer

Length: 1308 bp
ATGGGGGTTGTCGAAGGCCGGCTGGGAGGGTTTCTAATTGTTTTGACAATAATGGGGTTGGCTTTTCCGGTGTTCTTTAATGCACTGGTCCAGGGGTATAACCGTCCACCGCCGCGCAAAAACGTCTATGTCCCTTCTGATGATCCTGGTTCTGATTCTCCACAACAGGTGCATATATCTACAGTAGGTGCAGACAAAATGAGAGTATCCTGGATCACCGAGAGTCCAAGTTCAGCAACGGTGGAATATGGCACATCTCCTGGTGCTTATGAGCAGTCAGCAACTGGGTCGACAAGTTCATACAAGTATTTGATGTACGAGTCTGGGGAAATCCATGACGTGGTCATCGGTCCCTTGAAACCCAACACAGTATACTACTACCGTTGTGGATCCAATTCGGGTCCTGAATTCAGTTTCAAAACCCCTCCTGCATCCTTCCCTATCACATTTGCAGTCGTAGGTGATCTTGGGCAAACTGAATGGACTAAATCAACCCTCGATCACATATCCAAAGTAAACTACGACATGTTTATGCTACCAGGAGACTTATCCTATGCAGACGTGGACCAACCTTTATGGGACTCATTTGGGCGTCTGGTGCAGCCCTTGGCAAGCCAGCGGCCTTGGATGGTGACACAAGGCAATCACGAAATCGAGAAAATCCCTGTCCTGCACACTCATGCCTTCACTGCGTACAATGCAAGGTGGCACATGCCGTACGAAGAGAGTGGTTCGGATTCGAACCTCTACTACTCATTCGATGTTGCTGGAGTTCATGTGATCATGTTGGGGTCGTACACCGATTTTGATCCTAGTTCTGCTCAGTACCAGTGGTTGCAAGTAGATTTGGGGAAGGTGGATAGGGGAAGGACCCCGTGGATCGTTGTGCTTATCCATGCTCCTTGGTACAATTCTAACAAGGCTCACCAGGGTGAATCTGAATCTATTGACATGAAGGACGCCATGGAAGATCTGCTCTATCAAGCTCGTGTCGATGTTGTTTTAGCTGGCCATGTACATGCATATGAGCGTTTTACCCGCGTTTACAAGGATGAAGCCAATAATTGTGGTCCAGTGCATATTACCATTGGAGATGGTGGCAACCGTGAAGGTCTTGCTAGCAAGTATAAGAATCCGCAACCCAAAATATCGGTTTTCAGGGAGGCCAGCTTTGGGCACGGGCAATTAGTGGTGGCAAATGCAACTCATGCACAGTGGACATGGCATAGGAATGAAGATGATGAGGCAGTTGCCAGTGACTCCATTTGGCTCACAAGCCTCTCCTCTGATCCTGCCTGCAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

436

Amino Acids

48.38

Weight (kDa)

5.32

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013245)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52780 AT3G52780
fragaria_vesca FvH4_6g18430 FvH4_6g18430
malus_domestica MD12G1023400.v1.1 MD14G1020400.v1.1
prunus_persica Prupe.7G113100_v2.0.a1
pyrus_communis pycom12g02180
rosa_chinensis RchiOBHm_Chr3g0472961
rosa_laevigata RLG00000024044
rosa_multiflora Rmu_sc0021557.1_g000005
rosa_roxburghii Rroxscaffold_6G00408460
rosa_rugosa Rorug03G0129900
rosa_samantha Rh3AG182000 Rh3BG208300 Rh3CG205300 Rh3DG167800
rosa_wichuraiana Rw3G016150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 340
AccI GTMKAC 2 cut(s) 290, 372
AccII CGCG 2 cut(s) 115, 1041
AciI CCGC 5 cut(s) 110, 113, 619, 1039, 1136
AclWI GGATC 7 cut(s) 137, 221, 384, 397, 803, 887, 1283
AcoI YGGCCR 1 cut(s) 1009
AcsI RAATTY 1 cut(s) 407
AcyI GRCGYC 2 cut(s) 592, 960
AfaI GTAC 7 cut(s) 317, 695, 719, 797, 827, 908, 1017
AfiI CCNNNNNNNGG 2 cut(s) 340, 1173
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 2 cut(s) 358, 418
AjiI CACGTC 2 cut(s) 340, 562
AjnI CCWGG 6 cut(s) 90, 145, 209, 259, 538, 927
AleI CACNNNNGTG 1 cut(s) 930
AluBI AGCT 3 cut(s) 986, 1007, 1170
AluI AGCT 3 cut(s) 986, 1007, 1170
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 7 cut(s) 137, 221, 384, 397, 803, 887, 1283
AlwNI CAGNNNCTG 1 cut(s) 284
AoxI GGCC 4 cut(s) 16, 620, 1009, 1164
ApeKI GCWGC 1 cut(s) 601
ApoI RAATTY 1 cut(s) 407
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 6 cut(s) 88, 350, 401, 565, 870, 1070
AsuHPI GGTGA 5 cut(s) 208, 473, 643, 917, 944
AsuII TTCGAA 1 cut(s) 740
AsuNHI GCTAGC 1 cut(s) 1118
AvaII GGWCC 6 cut(s) 88, 350, 401, 565, 870, 1070
BaeGI GKGCMC 1 cut(s) 1179
BalI TGGCCA 1 cut(s) 1011
BamHI GGATCC 1 cut(s) 389
BauI CACGAG 1 cut(s) 987
BbvI GCAGC 1 cut(s) 613
BccI CCATC 2 cut(s) 622, 1088
BceAI ACGGC 1 cut(s) 700
BcgI CGANNNNNNTGC 2 cut(s) 270, 304
BciT130I CCWGG 6 cut(s) 92, 147, 211, 261, 540, 929
BciVI GTATCC 1 cut(s) 217
BclI TGATCA 1 cut(s) 780
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 2 cut(s) 813, 1119
BfmI CTRYAG 1 cut(s) 180
BfuI GTATCC 1 cut(s) 217
BglII AGATCT 1 cut(s) 970
BisI GCNGC 3 cut(s) 113, 602, 620
BlsI GCNGC 3 cut(s) 114, 603, 621
Bme1390I CCNGG 6 cut(s) 92, 147, 211, 261, 540, 929
Bme18I GGWCC 6 cut(s) 88, 350, 401, 565, 870, 1070
BmgBI CACGTC 2 cut(s) 340, 562
BmgT120I GGNCC 6 cut(s) 88, 350, 401, 565, 870, 1070
BmiI GGNNCC 4 cut(s) 352, 391, 402, 872
BmrFI CCNGG 6 cut(s) 92, 147, 211, 261, 540, 929
BmrI ACTGGG 1 cut(s) 294
BmsI GCATC 1 cut(s) 440
BmtI GCTAGC 1 cut(s) 1122
BmuI ACTGGG 1 cut(s) 294
BpmI CTGGAG 1 cut(s) 789
Bpu14I TTCGAA 1 cut(s) 740
BsaBI GATNNNNATC 1 cut(s) 807
BsaHI GRCGYC 2 cut(s) 592, 960
BsaJI CCNNGG 7 cut(s) 91, 606, 623, 875, 902, 928, 963
BsaWI WCCGGW 1 cut(s) 67
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 1173
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 5 cut(s) 90, 289, 829, 1073, 1254
Bse8I GATNNNNATC 1 cut(s) 807
BseBI CCWGG 6 cut(s) 92, 147, 211, 261, 540, 929
BseDI CCNNGG 7 cut(s) 91, 606, 623, 875, 902, 928, 963
BseGI GGATG 3 cut(s) 431, 633, 1057
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 2 cut(s) 340, 1173
BseMII CTCAG 1 cut(s) 836
BseNI ACTGG 5 cut(s) 90, 289, 829, 1073, 1254
BseRI GAGGAG 1 cut(s) 1273
BseSI GKGCMC 1 cut(s) 1179
BseXI GCAGC 1 cut(s) 613
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 3 cut(s) 210, 620, 656
Bsh1236I CGCG 2 cut(s) 115, 1041
BshFI GGCC 4 cut(s) 18, 622, 1011, 1166
BsiSI CCGG 2 cut(s) 19, 68
BsiWI CGTACG 1 cut(s) 717
BslFI GGGAC 3 cut(s) 116, 336, 593
BslI CCNNNNNNNGG 2 cut(s) 340, 1173
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 3 cut(s) 116, 336, 593
BsnI GGCC 4 cut(s) 18, 622, 1011, 1166
Bsp119I TTCGAA 1 cut(s) 740
Bsp1286I GDGCHC 1 cut(s) 1179
Bsp1407I TGTACA 1 cut(s) 1015
Bsp19I CCATGG 1 cut(s) 963
BspACI CCGC 5 cut(s) 110, 113, 619, 1039, 1136
BspANI GGCC 4 cut(s) 18, 622, 1011, 1166
BspCNI CTCAG 1 cut(s) 835
BspFNI CGCG 2 cut(s) 115, 1041
BspLI GGNNCC 4 cut(s) 352, 391, 402, 872
BspOI GCTAGC 1 cut(s) 1122
BspPI GGATC 7 cut(s) 137, 221, 384, 397, 803, 887, 1283
BspT104I TTCGAA 1 cut(s) 740
BsrFI RCCGGY 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 1015
BsrI ACTGG 5 cut(s) 90, 289, 829, 1073, 1254
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 7 cut(s) 91, 606, 623, 875, 902, 928, 963
BssNAI GTATAC 1 cut(s) 373
BssNI GRCGYC 2 cut(s) 592, 960
BssSI CACGAG 1 cut(s) 987
BssT1I CCWWGG 4 cut(s) 606, 623, 902, 963
Bst1107I GTATAC 1 cut(s) 373
Bst2BI CACGAG 1 cut(s) 987
Bst2UI CCWGG 6 cut(s) 92, 147, 211, 261, 540, 929
Bst4CI ACNGT 7 cut(s) 104, 184, 241, 370, 383, 1106, 1215
Bst6I CTCTTC 1 cut(s) 717
BstACI GRCGYC 2 cut(s) 592, 960
BstAPI GCANNNNNTGC 1 cut(s) 1208
BstAUI TGTACA 1 cut(s) 1015
BstBI TTCGAA 1 cut(s) 740
BstC8I GCNNGC 7 cut(s) 20, 613, 617, 1009, 1120, 1168, 1297
BstDEI CTNAG 1 cut(s) 822
BstDSI CCRYGG 2 cut(s) 875, 963
BstF5I GGATG 3 cut(s) 431, 633, 1057
BstFNI CGCG 2 cut(s) 115, 1041
BstHHI GCGC 1 cut(s) 117
BstMAI GTCTC 1 cut(s) 537
BstMWI GCNNNNNNNGC 4 cut(s) 598, 689, 698, 1208
BstNI CCWGG 6 cut(s) 92, 147, 211, 261, 540, 929
BstNSI RCATGY 3 cut(s) 529, 715, 1022
BstSCI CCNGG 6 cut(s) 90, 145, 209, 259, 538, 927
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 1 cut(s) 1179
BstUI CGCG 2 cut(s) 115, 1041
BstV1I GCAGC 1 cut(s) 613
BstX2I RGATCY 2 cut(s) 389, 970
BstYI RGATCY 2 cut(s) 389, 970
BstZ17I GTATAC 1 cut(s) 373
BsuI GTATCC 1 cut(s) 217
BsuRI GGCC 4 cut(s) 18, 622, 1011, 1166
BtgI CCRYGG 2 cut(s) 875, 963
BtrI CACGTC 2 cut(s) 340, 562
BtsCI GGATG 3 cut(s) 431, 633, 1057
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 6 cut(s) 83, 687, 836, 1080, 1220, 1261
Cac8I GCNNGC 7 cut(s) 20, 613, 617, 1009, 1120, 1168, 1297
CaiI CAGNNNCTG 1 cut(s) 284
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 18
Cfr13I GGNCC 6 cut(s) 88, 350, 401, 565, 870, 1070
CseI GACGC 2 cut(s) 581, 968
Csp6I GTAC 7 cut(s) 316, 694, 718, 796, 826, 907, 1016
CspCI CAANNNNNGTGG 2 cut(s) 1048, 1083
CviQI GTAC 7 cut(s) 316, 694, 718, 796, 826, 907, 1016
DdeI CTNAG 1 cut(s) 822
EaeI YGGCCR 1 cut(s) 1009
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
Eco130I CCWWGG 4 cut(s) 606, 623, 902, 963
Eco47I GGWCC 6 cut(s) 88, 350, 401, 565, 870, 1070
EcoO109I RGGNCCY 2 cut(s) 401, 870
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 6 cut(s) 90, 145, 209, 259, 538, 927
EcoT14I CCWWGG 4 cut(s) 606, 623, 902, 963
EcoT22I ATGCAT 1 cut(s) 1024
ErhI CCWWGG 4 cut(s) 606, 623, 902, 963
FaqI GGGAC 3 cut(s) 116, 336, 593
FauI CCCGC 1 cut(s) 1046
FauNDI CATATG 1 cut(s) 1024
FbaI TGATCA 1 cut(s) 780
FblI GTMKAC 2 cut(s) 290, 372
Fnu4HI GCNGC 3 cut(s) 113, 602, 620
FokI GGATG 3 cut(s) 418, 640, 1064
Fsp4HI GCNGC 3 cut(s) 113, 602, 620
FspBI CTAG 2 cut(s) 813, 1119
GlaI GCGC 1 cut(s) 116
GluI GCNGC 3 cut(s) 113, 602, 620
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 789
HaeIII GGCC 4 cut(s) 18, 622, 1011, 1166
HapII CCGG 2 cut(s) 19, 68
HgaI GACGC 2 cut(s) 581, 968
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 2 cut(s) 592, 960
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 9 cut(s) 155, 223, 320, 581, 737, 935, 941, 1132, 1259
HpaII CCGG 2 cut(s) 19, 68
HphI GGTGA 5 cut(s) 208, 473, 643, 917, 944
Hpy166II GTNNAC 8 cut(s) 107, 291, 373, 517, 565, 798, 1045, 1218
Hpy188I TCNGA 5 cut(s) 139, 154, 736, 940, 1288
Hpy188III TCNNGA 3 cut(s) 404, 647, 655
Hpy8I GTNNAC 8 cut(s) 107, 291, 373, 517, 565, 798, 1045, 1218
HpyAV CCTTC 8 cut(s) 8, 144, 445, 694, 847, 861, 949, 1103
HpyCH4III ACNGT 7 cut(s) 104, 184, 241, 370, 383, 1106, 1215
HpyCH4IV ACGT 3 cut(s) 123, 339, 561
HpyF10VI GCNNNNNNNGC 4 cut(s) 598, 689, 698, 1208
HpyF3I CTNAG 1 cut(s) 822
HpySE526I ACGT 3 cut(s) 123, 339, 561
Hsp92I GRCGYC 2 cut(s) 592, 960
HspAI GCGC 1 cut(s) 115
KroI GCCGGC 1 cut(s) 18
KroNI GCCGGC 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 780
LmnI GCTCC 1 cut(s) 904
Lsp1109I GCAGC 1 cut(s) 613
LweI GCATC 1 cut(s) 440
MaeI CTAG 2 cut(s) 813, 1119
MaeII ACGT 3 cut(s) 123, 339, 561
MaeIII GTNAC 2 cut(s) 631, 1256
MboII GAAGA 3 cut(s) 734, 980, 1247
MflI RGATCY 2 cut(s) 389, 970
MhlI GDGCHC 1 cut(s) 1179
MlsI TGGCCA 1 cut(s) 1011
MluCI AATT 7 cut(s) 36, 394, 407, 910, 1063, 1184, 1303
MluNI TGGCCA 1 cut(s) 1011
MlyI GAGTC 4 cut(s) 232, 329, 575, 1253
MnlI CCTC 8 cut(s) 20, 435, 506, 755, 1156, 1237, 1289, 1294
Mox20I TGGCCA 1 cut(s) 1011
Mph1103I ATGCAT 1 cut(s) 1024
MroNI GCCGGC 1 cut(s) 18
MscI TGGCCA 1 cut(s) 1011
MseI TTAA 2 cut(s) 78, 1306
MslI CAYNNNNRTG 6 cut(s) 530, 678, 930, 1017, 1023, 1230
Msp20I TGGCCA 1 cut(s) 1011
MspA1I CMGCKG 1 cut(s) 619
MspI CCGG 2 cut(s) 19, 68
MspR9I CCNGG 6 cut(s) 92, 147, 211, 261, 540, 929
MvaI CCWGG 6 cut(s) 92, 147, 211, 261, 540, 929
MvnI CGCG 2 cut(s) 115, 1041
MwoI GCNNNNNNNGC 4 cut(s) 598, 689, 698, 1208
NaeI GCCGGC 1 cut(s) 20
NcoI CCATGG 1 cut(s) 963
NdeI CATATG 1 cut(s) 1024
NgoMIV GCCGGC 1 cut(s) 18
NheI GCTAGC 1 cut(s) 1118
NlaIV GGNNCC 4 cut(s) 352, 391, 402, 872
NmuCI GTSAC 2 cut(s) 631, 1256
NsiI ATGCAT 1 cut(s) 1024
NspI RCATGY 3 cut(s) 529, 715, 1022
NspV TTCGAA 1 cut(s) 740
OliI CACNNNNGTG 1 cut(s) 930
PciI ACATGT 1 cut(s) 525
PdiI GCCGGC 1 cut(s) 20
PfeI GAWTC 5 cut(s) 155, 737, 935, 941, 1132
Pfl23II CGTACG 1 cut(s) 717
PflFI GACNNNGTC 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 340
PfoI TCCNGGA 1 cut(s) 209
PkrI GCNGC 3 cut(s) 114, 603, 621
PleI GAGTC 4 cut(s) 231, 328, 575, 1253
PpsI GAGTC 4 cut(s) 231, 328, 575, 1253
PpuMI RGGWCCY 2 cut(s) 401, 870
PscI ACATGT 1 cut(s) 525
Psp5II RGGWCCY 2 cut(s) 401, 870
Psp6I CCWGG 6 cut(s) 90, 145, 209, 259, 538, 927
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 6 cut(s) 90, 145, 209, 259, 538, 927
PspLI CGTACG 1 cut(s) 717
PspN4I GGNNCC 4 cut(s) 352, 391, 402, 872
PspPI GGNCC 6 cut(s) 88, 350, 401, 565, 870, 1070
PspPPI RGGWCCY 2 cut(s) 401, 870
PsrI GAACNNNNNNTAC 2 cut(s) 734, 766
PstNI CAGNNNCTG 1 cut(s) 284
PsuI RGATCY 2 cut(s) 389, 970
PsyI GACNNNGTC 1 cut(s) 341
RsaI GTAC 7 cut(s) 317, 695, 719, 797, 827, 908, 1017
RsaNI GTAC 7 cut(s) 316, 694, 718, 796, 826, 907, 1016
RseI CAYNNNNRTG 6 cut(s) 530, 678, 930, 1017, 1023, 1230
SalI GTCGAC 1 cut(s) 289
SaqAI TTAA 2 cut(s) 78, 1306
SatI GCNGC 3 cut(s) 113, 602, 620
Sau96I GGNCC 6 cut(s) 88, 350, 401, 565, 870, 1070
SchI GAGTC 4 cut(s) 232, 329, 575, 1253
ScrFI CCNGG 6 cut(s) 92, 147, 211, 261, 540, 929
SduI GDGCHC 1 cut(s) 1179
SfaNI GCATC 1 cut(s) 440
SfcI CTRYAG 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 740
SinI GGWCC 6 cut(s) 88, 350, 401, 565, 870, 1070
SmiMI CAYNNNNRTG 6 cut(s) 530, 678, 930, 1017, 1023, 1230
Sse9I AATT 7 cut(s) 36, 394, 407, 910, 1063, 1184, 1303
SsiI CCGC 5 cut(s) 110, 113, 619, 1039, 1136
SspMI CTAG 2 cut(s) 813, 1119
StyD4I CCNGG 6 cut(s) 90, 145, 209, 259, 538, 927
StyI CCWWGG 4 cut(s) 606, 623, 902, 963
TaaI ACNGT 7 cut(s) 104, 184, 241, 370, 383, 1106, 1215
TaiI ACGT 3 cut(s) 126, 342, 564
TaqI TCGA 7 cut(s) 12, 290, 498, 654, 740, 759, 993
TaqII GACCGA 1 cut(s) 338
TasI AATT 7 cut(s) 36, 394, 407, 910, 1063, 1184, 1303
TatI WGTACW 1 cut(s) 1015
TauI GCSGC 2 cut(s) 115, 622
TfiI GAWTC 5 cut(s) 155, 737, 935, 941, 1132
Tru1I TTAA 2 cut(s) 78, 1306
Tru9I TTAA 2 cut(s) 78, 1306
TscAI CASTG 6 cut(s) 90, 694, 836, 1080, 1220, 1261
TseFI GTSAC 2 cut(s) 631, 1256
TseI GCWGC 1 cut(s) 601
Tsp45I GTSAC 2 cut(s) 631, 1256
TspDTI ATGAA 5 cut(s) 288, 764, 968, 1068, 1248
TspRI CASTG 6 cut(s) 90, 694, 836, 1080, 1220, 1261
Tth111I GACNNNGTC 1 cut(s) 341
Van91I CCANNNNNTGG 1 cut(s) 340
VpaK11BI GGWCC 6 cut(s) 88, 350, 401, 565, 870, 1070
XapI RAATTY 1 cut(s) 407
XceI RCATGY 3 cut(s) 529, 715, 1022
XmiI GTMKAC 2 cut(s) 290, 372
XspI CTAG 2 cut(s) 813, 1119
Zsp2I ATGCAT 1 cut(s) 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.