Rh3AG182000

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0013245
Physical Location & Seq
Forward (+)
17134266 .. 17138033
3768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG182000.1

Sequence Viewer

Length: 1113 bp
ATGAGAGTATCATGGACGACAAAGAACCCAAGTCCAGCAACAGTGGAATACGGCACATCTCCTGGTGCTTATTCAAGCTCTGCAACTGGAAGTACAACTTCATACTCCTATTTATTGTACCACAGTGGAGAATTCCATCATGTTGACATCGGTCCATTGAATGCCAACACAGTATACTACTACCGCTGCGGCTCCAGTCCGGGTCCTGAGTTCAGTTTCAAAACCCCTCCTGCTGCCTTCCCTCTCACATTTGCAGTGGCAGGTGATCTTGGACAGACTGAGTGGACTAACGAAACCCTCCAACACATAAGCAAATCACCATATGACGTACTCCTATTGCCCGGAGACTTGTCCTATGCTGATTTCAAGCAAAATTTATGGGACTCATTCGGCCGATTGGTCCAGCCACTGGCAAGCCAGCGGCCGTGGATGGTCACAGAAGGCAACCATGAGGTCGAGAAGATCCCAGTGGTGCATTCTGAACCCTTCACGGCATACAACGCCAGGTGGAGAATGCCGTTCGAACAGAGTGGGTCGGAATCCAACCTCTACTATTCCTTCAATGTGGCAGGAGTCCATGTTATCATGTTGGGTTCATACACCGATTTTGATCCTGCTTCTTTACAATACAAGTGGCTACAGACGGATTTGGCAAAGGTTGACAGGGGGAGAACTCCTTGGGTTGTGGTGCTTATCCATGCCCCATGGTACAACTCTAACGAAGCTCATCAGGGTGAATCCGAATCAATTGACATGAAGAGTTCCATGGAAGAATTACTCTATAAAGGTCGTGTTGATCTTGTTTTTGCAGGACATGTACATGCCTATGAGCGTTTCACTCGAGTTTACAAAGACAAAGCAGATGATTGTGGTCCGGTGCATATTACTATTGGAGATGGAGGCAACCGCGAAGGACTTGCCACAAAGTATGAAGAACCAAAACCGGCAATATCAATGTTCAGGGAGGCGAGCTTTGGGCATGGACAGTTGCAGGTAGTGAATGGAACCCATGCGCTGTGGACATGGCGTACGAATCAGGATGATAATGAGACTGTTGCAAGTGATAGCATTTGGCTCACAAGCCTCTTGGCTACTCCTGCTTGTAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

370

Amino Acids

41.42

Weight (kDa)

5.58

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 1 - 75 2.2e-17 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 83 - 276 5.3e-20 Calcineurin-like phosphoesterase
Metallophos_C PF14008 291 - 351 1.1e-19 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013245)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52780 AT3G52780
fragaria_vesca FvH4_6g18430 FvH4_6g18430
malus_domestica MD12G1023400.v1.1 MD14G1020400.v1.1
prunus_persica Prupe.7G113100_v2.0.a1
pyrus_communis pycom12g02180
rosa_chinensis RchiOBHm_Chr3g0472961
rosa_laevigata RLG00000024044
rosa_multiflora Rmu_sc0021557.1_g000005
rosa_roxburghii Rroxscaffold_6G00408460
rosa_rugosa Rorug03G0129900
rosa_samantha Rh3AG182000 Rh3BG208300 Rh3CG205300 Rh3DG167800
rosa_wichuraiana Rw3G016150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 251
Acc36I ACCTGC 2 cut(s) 251, 982
AccB7I CCANNNNNTGG 1 cut(s) 409
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 909
AciI CCGC 4 cut(s) 184, 189, 421, 907
AclWI GGATC 2 cut(s) 457, 605
AcoI YGGCCR 2 cut(s) 391, 422
AcsI RAATTY 2 cut(s) 131, 373
AfaI GTAC 6 cut(s) 94, 119, 330, 710, 819, 1030
AfiI CCNNNNNNNGG 1 cut(s) 409
AflIII ACRYGT 1 cut(s) 814
AgsI TTSAA 5 cut(s) 75, 160, 220, 367, 562
AjnI CCWGG 2 cut(s) 61, 503
AluBI AGCT 3 cut(s) 78, 725, 972
AluI AGCT 3 cut(s) 78, 725, 972
Alw26I GTCTC 2 cut(s) 339, 1043
AlwI GGATC 2 cut(s) 457, 605
AlwNI CAGNNNCTG 1 cut(s) 409
Ama87I CYCGRG 1 cut(s) 840
AoxI GGCC 2 cut(s) 391, 422
ApeKI GCWGC 2 cut(s) 186, 233
ApoI RAATTY 2 cut(s) 131, 373
AspLEI GCGC 1 cut(s) 1015
AspS9I GGNCC 4 cut(s) 152, 203, 400, 872
AsuC2I CCSGG 2 cut(s) 201, 342
AsuHPI GGTGA 3 cut(s) 275, 309, 746
AsuII TTCGAA 1 cut(s) 522
AvaI CYCGRG 1 cut(s) 840
AvaII GGWCC 4 cut(s) 152, 203, 400, 872
BbvI GCAGC 2 cut(s) 173, 220
BccI CCATC 3 cut(s) 144, 424, 890
BceAI ACGGC 4 cut(s) 67, 409, 502, 507
BcgI CGANNNNNNTGC 2 cut(s) 899, 933
BciT130I CCWGG 2 cut(s) 63, 505
BcnI CCSGG 2 cut(s) 201, 342
BcoDI GTCTC 2 cut(s) 339, 1043
BfmI CTRYAG 1 cut(s) 638
BfuAI ACCTGC 2 cut(s) 251, 982
BisI GCNGC 4 cut(s) 187, 190, 234, 422
BlsI GCNGC 4 cut(s) 188, 191, 235, 423
Bme1390I CCNGG 4 cut(s) 63, 201, 342, 505
Bme18I GGWCC 4 cut(s) 152, 203, 400, 872
BmeT110I CYCGRG 1 cut(s) 840
BmgT120I GGNCC 4 cut(s) 152, 203, 400, 872
BmiI GGNNCC 3 cut(s) 193, 204, 1006
BmrFI CCNGG 4 cut(s) 63, 201, 342, 505
BmrI ACTGGG 1 cut(s) 461
BmuI ACTGGG 1 cut(s) 461
BoxI GACNNNNGTC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 178
Bpu14I TTCGAA 1 cut(s) 522
BpuMI CCSGG 2 cut(s) 201, 342
BsaBI GATNNNNATC 1 cut(s) 609
BsaJI CCNNGG 4 cut(s) 425, 677, 704, 765
BsaWI WCCGGW 1 cut(s) 874
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 409
Bse118I RCCGGY 1 cut(s) 943
Bse1I ACTGG 4 cut(s) 91, 195, 414, 467
Bse8I GATNNNNATC 1 cut(s) 609
BseBI CCWGG 2 cut(s) 63, 505
BseDI CCNNGG 4 cut(s) 425, 677, 704, 765
BseGI GGATG 2 cut(s) 435, 1045
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 1 cut(s) 409
BseMII CTCAG 2 cut(s) 198, 270
BseNI ACTGG 4 cut(s) 91, 195, 414, 467
BseX3I CGGCCG 2 cut(s) 391, 422
BseXI GCAGC 2 cut(s) 173, 220
Bsh1236I CGCG 1 cut(s) 909
Bsh1285I CGRYCG 2 cut(s) 394, 425
BshFI GGCC 2 cut(s) 393, 424
BsiEI CGRYCG 2 cut(s) 394, 425
BsiHKCI CYCGRG 1 cut(s) 840
BsiSI CCGG 4 cut(s) 200, 342, 875, 944
BsiWI CGTACG 1 cut(s) 1028
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 409
BsmAI GTCTC 2 cut(s) 339, 1043
BsmFI GGGAC 1 cut(s) 395
BsmI GAATGC 3 cut(s) 166, 475, 519
BsnI GGCC 2 cut(s) 393, 424
BsoBI CYCGRG 1 cut(s) 840
Bsp119I TTCGAA 1 cut(s) 522
Bsp1407I TGTACA 1 cut(s) 817
Bsp143I GATC 4 cut(s) 265, 462, 610, 796
Bsp19I CCATGG 2 cut(s) 704, 765
BspACI CCGC 4 cut(s) 184, 189, 421, 907
BspANI GGCC 2 cut(s) 393, 424
BspCNI CTCAG 2 cut(s) 199, 271
BspFNI CGCG 1 cut(s) 909
BspLI GGNNCC 3 cut(s) 193, 204, 1006
BspMI ACCTGC 2 cut(s) 251, 982
BspPI GGATC 2 cut(s) 457, 605
BspT104I TTCGAA 1 cut(s) 522
BsrFI RCCGGY 1 cut(s) 943
BsrGI TGTACA 1 cut(s) 817
BsrI ACTGG 4 cut(s) 91, 195, 414, 467
BssAI RCCGGY 1 cut(s) 943
BssECI CCNNGG 4 cut(s) 425, 677, 704, 765
BssMI GATC 4 cut(s) 265, 462, 610, 796
BssNAI GTATAC 1 cut(s) 175
BssT1I CCWWGG 3 cut(s) 677, 704, 765
Bst1107I GTATAC 1 cut(s) 175
Bst2UI CCWGG 2 cut(s) 63, 505
Bst4CI ACNGT 5 cut(s) 43, 125, 172, 987, 1054
Bst6I CTCTTC 1 cut(s) 752
BstAUI TGTACA 1 cut(s) 817
BstBI TTCGAA 1 cut(s) 522
BstC8I GCNNGC 3 cut(s) 415, 419, 970
BstDEI CTNAG 2 cut(s) 207, 279
BstDSI CCRYGG 3 cut(s) 425, 704, 765
BstF5I GGATG 2 cut(s) 435, 1045
BstFNI CGCG 1 cut(s) 909
BstHHI GCGC 1 cut(s) 1015
BstKTI GATC 4 cut(s) 268, 465, 613, 799
BstMAI GTCTC 2 cut(s) 339, 1043
BstMBI GATC 4 cut(s) 265, 462, 610, 796
BstMCI CGRYCG 2 cut(s) 394, 425
BstMWI GCNNNNNNNGC 2 cut(s) 500, 1097
BstNI CCWGG 2 cut(s) 63, 505
BstNSI RCATGY 2 cut(s) 818, 824
BstPAI GACNNNNGTC 1 cut(s) 150
BstSCI CCNGG 4 cut(s) 61, 199, 340, 503
BstSFI CTRYAG 1 cut(s) 638
BstUI CGCG 1 cut(s) 909
BstV1I GCAGC 2 cut(s) 173, 220
BstX2I RGATCY 1 cut(s) 462
BstYI RGATCY 1 cut(s) 462
BstZ17I GTATAC 1 cut(s) 175
BstZI CGGCCG 2 cut(s) 391, 422
BsuRI GGCC 2 cut(s) 393, 424
BtgI CCRYGG 3 cut(s) 425, 704, 765
BtsCI GGATG 2 cut(s) 435, 1045
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 5 cut(s) 48, 130, 261, 407, 474
BveI ACCTGC 2 cut(s) 251, 982
Cac8I GCNNGC 3 cut(s) 415, 419, 970
CaiI CAGNNNCTG 1 cut(s) 409
CfoI GCGC 1 cut(s) 1015
Cfr10I RCCGGY 1 cut(s) 943
Cfr13I GGNCC 4 cut(s) 152, 203, 400, 872
Csp6I GTAC 6 cut(s) 93, 118, 329, 709, 818, 1029
CspCI CAANNNNNGTGG 2 cut(s) 614, 649
CviQI GTAC 6 cut(s) 93, 118, 329, 709, 818, 1029
DdeI CTNAG 2 cut(s) 207, 279
DpnI GATC 4 cut(s) 267, 464, 612, 798
DpnII GATC 4 cut(s) 265, 462, 610, 796
EaeI YGGCCR 2 cut(s) 391, 422
EagI CGGCCG 2 cut(s) 391, 422
Eam1104I CTCTTC 1 cut(s) 752
EarI CTCTTC 1 cut(s) 752
EclXI CGGCCG 2 cut(s) 391, 422
Eco130I CCWWGG 3 cut(s) 677, 704, 765
Eco47I GGWCC 4 cut(s) 152, 203, 400, 872
Eco52I CGGCCG 2 cut(s) 391, 422
Eco88I CYCGRG 1 cut(s) 840
EcoO109I RGGNCCY 1 cut(s) 203
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 61, 503
EcoT14I CCWWGG 3 cut(s) 677, 704, 765
ErhI CCWWGG 3 cut(s) 677, 704, 765
FalI AAGNNNNNCTT 2 cut(s) 82, 114
FaqI GGGAC 1 cut(s) 395
FauNDI CATATG 1 cut(s) 322
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 4 cut(s) 187, 190, 234, 422
FokI GGATG 2 cut(s) 442, 1052
Fsp4HI GCNGC 4 cut(s) 187, 190, 234, 422
GlaI GCGC 1 cut(s) 1014
GluI GCNGC 4 cut(s) 187, 190, 234, 422
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 2 cut(s) 393, 424
HapII CCGG 4 cut(s) 200, 342, 875, 944
HhaI GCGC 1 cut(s) 1015
Hin6I GCGC 1 cut(s) 1013
HinP1I GCGC 1 cut(s) 1013
HincII GTYRAC 2 cut(s) 145, 661
HindII GTYRAC 2 cut(s) 145, 661
HinfI GANTC 6 cut(s) 383, 539, 573, 737, 743, 1033
HpaII CCGG 4 cut(s) 200, 342, 875, 944
HphI GGTGA 3 cut(s) 275, 309, 746
Hpy166II GTNNAC 6 cut(s) 145, 175, 285, 661, 847, 1020
Hpy188I TCNGA 3 cut(s) 481, 538, 742
Hpy188III TCNNGA 3 cut(s) 206, 457, 1037
Hpy8I GTNNAC 6 cut(s) 145, 175, 285, 661, 847, 1020
HpyAV CCTTC 5 cut(s) 247, 434, 496, 568, 905
HpyCH4III ACNGT 5 cut(s) 43, 125, 172, 987, 1054
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 7 cut(s) 83, 254, 475, 809, 880, 991, 1058
HpyF10VI GCNNNNNNNGC 2 cut(s) 500, 1097
HpyF3I CTNAG 2 cut(s) 207, 279
HpySE526I ACGT 1 cut(s) 327
HspAI GCGC 1 cut(s) 1013
Kzo9I GATC 4 cut(s) 265, 462, 610, 796
LmnI GCTCC 1 cut(s) 197
Lsp1109I GCAGC 2 cut(s) 173, 220
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 433
MalI GATC 4 cut(s) 267, 464, 612, 798
MboI GATC 4 cut(s) 265, 462, 610, 796
MboII GAAGA 4 cut(s) 472, 769, 782, 944
MfeI CAATTG 1 cut(s) 747
MflI RGATCY 1 cut(s) 462
MluCI AATT 4 cut(s) 131, 373, 747, 773
MlyI GAGTC 2 cut(s) 377, 582
MmeI TCCRAC 3 cut(s) 325, 516, 567
MnlI CCTC 8 cut(s) 237, 252, 308, 445, 557, 893, 958, 1094
MslI CAYNNNNRTG 3 cut(s) 732, 819, 825
MspA1I CMGCKG 2 cut(s) 186, 421
MspI CCGG 4 cut(s) 200, 342, 875, 944
MspR9I CCNGG 4 cut(s) 63, 201, 342, 505
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 3 cut(s) 166, 475, 519
MvaI CCWGG 2 cut(s) 63, 505
MvnI CGCG 1 cut(s) 909
MwoI GCNNNNNNNGC 2 cut(s) 500, 1097
NciI CCSGG 2 cut(s) 201, 342
NcoI CCATGG 2 cut(s) 704, 765
NdeI CATATG 1 cut(s) 322
NdeII GATC 4 cut(s) 265, 462, 610, 796
NlaIV GGNNCC 3 cut(s) 193, 204, 1006
NmuCI GTSAC 1 cut(s) 433
NspI RCATGY 2 cut(s) 818, 824
NspV TTCGAA 1 cut(s) 522
PaeR7I CTCGAG 1 cut(s) 840
PaqCI CACCTGC 1 cut(s) 251
PciI ACATGT 1 cut(s) 814
PctI GAATGC 3 cut(s) 166, 475, 519
PfeI GAWTC 4 cut(s) 539, 737, 743, 1033
Pfl23II CGTACG 1 cut(s) 1028
PflMI CCANNNNNTGG 1 cut(s) 409
PkrI GCNGC 4 cut(s) 188, 191, 235, 423
PleI GAGTC 2 cut(s) 377, 581
PpsI GAGTC 2 cut(s) 377, 581
PpuMI RGGWCCY 1 cut(s) 203
PscI ACATGT 1 cut(s) 814
PshAI GACNNNNGTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 61, 503
PspGI CCWGG 2 cut(s) 61, 503
PspLI CGTACG 1 cut(s) 1028
PspN4I GGNNCC 3 cut(s) 193, 204, 1006
PspPI GGNCC 4 cut(s) 152, 203, 400, 872
PspPPI RGGWCCY 1 cut(s) 203
PspXI VCTCGAGB 1 cut(s) 840
PstNI CAGNNNCTG 1 cut(s) 409
PsuI RGATCY 1 cut(s) 462
RsaI GTAC 6 cut(s) 94, 119, 330, 710, 819, 1030
RsaNI GTAC 6 cut(s) 93, 118, 329, 709, 818, 1029
RseI CAYNNNNRTG 3 cut(s) 732, 819, 825
SatI GCNGC 4 cut(s) 187, 190, 234, 422
Sau3AI GATC 4 cut(s) 265, 462, 610, 796
Sau96I GGNCC 4 cut(s) 152, 203, 400, 872
SchI GAGTC 2 cut(s) 377, 582
ScrFI CCNGG 4 cut(s) 63, 201, 342, 505
SfcI CTRYAG 1 cut(s) 638
Sfr274I CTCGAG 1 cut(s) 840
SfuI TTCGAA 1 cut(s) 522
SinI GGWCC 4 cut(s) 152, 203, 400, 872
SlaI CTCGAG 1 cut(s) 840
SmiMI CAYNNNNRTG 3 cut(s) 732, 819, 825
SmlI CTYRAG 1 cut(s) 840
SmoI CTYRAG 1 cut(s) 840
Sse9I AATT 4 cut(s) 131, 373, 747, 773
SsiI CCGC 4 cut(s) 184, 189, 421, 907
StyD4I CCNGG 4 cut(s) 61, 199, 340, 503
StyI CCWWGG 3 cut(s) 677, 704, 765
TaaI ACNGT 5 cut(s) 43, 125, 172, 987, 1054
TaiI ACGT 1 cut(s) 330
TaqI TCGA 3 cut(s) 456, 522, 841
TaqII GACCGA 1 cut(s) 140
TasI AATT 4 cut(s) 131, 373, 747, 773
TatI WGTACW 2 cut(s) 92, 817
TauI GCSGC 2 cut(s) 192, 424
TfiI GAWTC 4 cut(s) 539, 737, 743, 1033
TscAI CASTG 5 cut(s) 48, 130, 261, 414, 474
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 2 cut(s) 186, 233
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 4 cut(s) 90, 585, 770, 945
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 5 cut(s) 48, 130, 261, 414, 474
Van91I CCANNNNNTGG 1 cut(s) 409
VpaK11BI GGWCC 4 cut(s) 152, 203, 400, 872
XapI RAATTY 2 cut(s) 131, 373
XceI RCATGY 2 cut(s) 818, 824
XhoI CTCGAG 1 cut(s) 840
XmiI GTMKAC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.