Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0019754

Physical Location & Seq

Genomic Coordinates Reverse (-)

3026735 .. 3036147

Gene Structure 9413 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1034100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 465 bp

ATGACATCACTGATTGATGCAATTCGTTCAGTTTATATGGGGTGTCAGTTGCTCAGTCTGATGAAAGTAGATGCTTCAGGTTTCGAAGAGCTCATGTATCAGCAAGTTCTGCGTAGGTTTGCCTATTTTAATGCTATACAAATAAGTGAGCCCGCCCCGCTGAAGGAGTTGATCGTGTTGGCACTAAAAGAGGGGGAGGATCCGGAATGCAGTGTGAATAATGAACCGAATGAGAAGATTGCAGAGTCTGGAAGAGATGACATTGTCATGACAGAAAATGAAATTGAGCATGAACTAGTTGCAGAGGCAGAGAGTGCATGGGCTCTGCGTGAATGGTCAATCCAGCATGTTTTGTTTCCATCAATGAGGTTCTCTTTCAAGCCACCAAATTCCATGGCTACAAACGGAGCATTTGTCCGGGTAGCTTCATTGGAGAAACTATACAGGACTTTCGGAAGATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000793 GO:0000794 GO:0000795 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005712 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009791 GO:0009845 GO:0009987 GO:0010154 GO:0016445 GO:0016446 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022414 GO:0031974 GO:0031981 GO:0032300 GO:0032389 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051716 GO:0061458 GO:0070013 GO:0071704 GO:0090304 GO:0090351 GO:0097159 GO:0099086 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko01524 ko03430 ko03460 ko05200 ko05210 ko05213 ko05226 map01524 map03430 map03460 map05200 map05210 map05213 map05226

Pfam Domains

Protein Families

DNA_mis_repair HATPase_c_3 Mlh1_C

Protein Analysis

155

Amino Acids

17.5

Weight (kDa)

4.82

Isoelectric Point (pI)

36.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mlh1_C	PF16413	28 - 83	4.5e-08	DNA mismatch repair protein Mlh1 C-terminus
Mlh1_C	PF16413	93 - 154	6.5e-13	DNA mismatch repair protein Mlh1 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0019754)

Species	Orthologous Gene IDs
malus_domestica	MD14G1034100.v1.1
pyrus_communis	pycom08g11520
rosa_multiflora	Rmu_sc0003722.1_g000005 Rmu_sc0039398.1_g000001
rosa_samantha	Rh7AG388500 Rh7AG388600 Rh7CG407800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	1 cut(s)	202
AciI	CCGC	2 cut(s)	153, 158
AclWI	GGATC	2 cut(s)	194, 207
AcsI	RAATTY	1 cut(s)	388
AcuI	CTGAAG	2 cut(s)	60, 182
AfiI	CCNNNNNNNGG	1 cut(s)	163
AgsI	TTSAA	1 cut(s)	379
AhlI	ACTAGT	1 cut(s)	295
AluBI	AGCT	2 cut(s)	91, 425
AluI	AGCT	2 cut(s)	91, 425
Alw21I	GWGCWC	1 cut(s)	93
AlwI	GGATC	2 cut(s)	194, 207
AlwNI	CAGNNNCTG	1 cut(s)	248
Aor13HI	TCCGGA	1 cut(s)	202
ApoI	RAATTY	1 cut(s)	388
AsuC2I	CCSGG	1 cut(s)	419
AsuII	TTCGAA	1 cut(s)	84
BamHI	GGATCC	1 cut(s)	199
BanII	GRGCYC	3 cut(s)	93, 153, 325
Bbv12I	GWGCWC	1 cut(s)	93
BccI	CCATC	1 cut(s)	367
BcnI	CCSGG	1 cut(s)	419
BcuI	ACTAGT	1 cut(s)	295
BfaI	CTAG	1 cut(s)	296
Bme1390I	CCNGG	1 cut(s)	419
BmiI	GGNNCC	1 cut(s)	201
BmrFI	CCNGG	1 cut(s)	419
BmsI	GCATC	3 cut(s)	7, 61, 449
Bpu14I	TTCGAA	1 cut(s)	84
BpuMI	CCSGG	1 cut(s)	419
BsaJI	CCNNGG	1 cut(s)	393
BsaWI	WCCGGW	1 cut(s)	202
BsaXI	ACNNNNNCTCC	2 cut(s)	399, 429
Bsc4I	CCNNNNNNNGG	1 cut(s)	163
BseAI	TCCGGA	1 cut(s)	202
BseDI	CCNNGG	1 cut(s)	393
BseLI	CCNNNNNNNGG	1 cut(s)	163
BseMII	CTCAG	1 cut(s)	67
BsiHKAI	GWGCWC	1 cut(s)	93
BsiSI	CCGG	2 cut(s)	203, 418
BslI	CCNNNNNNNGG	1 cut(s)	163
BsmI	GAATGC	1 cut(s)	212
Bsp119I	TTCGAA	1 cut(s)	84
Bsp1286I	GDGCHC	3 cut(s)	93, 153, 325
Bsp13I	TCCGGA	1 cut(s)	202
Bsp143I	GATC	2 cut(s)	171, 199
Bsp19I	CCATGG	1 cut(s)	393
BspACI	CCGC	2 cut(s)	153, 158
BspCNI	CTCAG	1 cut(s)	66
BspEI	TCCGGA	1 cut(s)	202
BspHI	TCATGA	1 cut(s)	267
BspLI	GGNNCC	1 cut(s)	201
BspPI	GGATC	2 cut(s)	194, 207
BspQI	GCTCTTC	1 cut(s)	81
BspT104I	TTCGAA	1 cut(s)	84
BssECI	CCNNGG	1 cut(s)	393
BssMI	GATC	2 cut(s)	171, 199
BssT1I	CCWWGG	1 cut(s)	393
Bst6I	CTCTTC	2 cut(s)	81, 247
BstAPI	GCANNNNNTGC	2 cut(s)	109, 314
BstBI	TTCGAA	1 cut(s)	84
BstC8I	GCNNGC	1 cut(s)	153
BstDEI	CTNAG	1 cut(s)	53
BstDSI	CCRYGG	1 cut(s)	393
BstKTI	GATC	2 cut(s)	174, 202
BstMBI	GATC	2 cut(s)	171, 199
BstMWI	GCNNNNNNNGC	3 cut(s)	109, 157, 314
BstNSI	RCATGY	1 cut(s)	350
BstSCI	CCNGG	1 cut(s)	417
BstX2I	RGATCY	1 cut(s)	199
BstYI	RGATCY	1 cut(s)	199
BtgI	CCRYGG	1 cut(s)	393
BtsI	GCAGTG	1 cut(s)	217
BtsIMutI	CAGTG	2 cut(s)	8, 217
Cac8I	GCNNGC	1 cut(s)	153
CaiI	CAGNNNCTG	1 cut(s)	248
CciI	TCATGA	1 cut(s)	267
CviAII	CATG	6 cut(s)	94, 268, 290, 318, 347, 394
CviJI	RGCY	6 cut(s)	91, 151, 323, 382, 398, 425
CviKI_1	RGCY	6 cut(s)	91, 151, 323, 382, 398, 425
DdeI	CTNAG	1 cut(s)	53
DpnI	GATC	2 cut(s)	173, 201
DpnII	GATC	2 cut(s)	171, 199
Eam1104I	CTCTTC	2 cut(s)	81, 247
EarI	CTCTTC	2 cut(s)	81, 247
Ecl136II	GAGCTC	1 cut(s)	91
Eco130I	CCWWGG	1 cut(s)	393
Eco24I	GRGCYC	3 cut(s)	93, 153, 325
Eco53kI	GAGCTC	1 cut(s)	91
Eco57I	CTGAAG	2 cut(s)	60, 182
EcoICRI	GAGCTC	1 cut(s)	91
EcoT14I	CCWWGG	1 cut(s)	393
EcoT38I	GRGCYC	3 cut(s)	93, 153, 325
ErhI	CCWWGG	1 cut(s)	393
FaeI	CATG	6 cut(s)	97, 271, 293, 321, 350, 397
FatI	CATG	6 cut(s)	93, 267, 289, 317, 346, 393
FauI	CCCGC	2 cut(s)	160, 165
FriOI	GRGCYC	3 cut(s)	93, 153, 325
FspBI	CTAG	1 cut(s)	296
HapII	CCGG	2 cut(s)	203, 418
Hin1II	CATG	6 cut(s)	97, 271, 293, 321, 350, 397
HinfI	GANTC	1 cut(s)	245
HpaII	CCGG	2 cut(s)	203, 418
Hpy188I	TCNGA	2 cut(s)	60, 455
Hpy188III	TCNNGA	3 cut(s)	203, 249, 268
HpyAV	CCTTC	1 cut(s)	157
HpyCH4V	TGCA	5 cut(s)	20, 210, 242, 302, 317
HpyF10VI	GCNNNNNNNGC	3 cut(s)	109, 157, 314
HpyF3I	CTNAG	1 cut(s)	53
Hsp92II	CATG	6 cut(s)	97, 271, 293, 321, 350, 397
Kpn2I	TCCGGA	1 cut(s)	202
Kzo9I	GATC	2 cut(s)	171, 199
LguI	GCTCTTC	1 cut(s)	81
LmnI	GCTCC	1 cut(s)	407
LpnPI	CCDG	6 cut(s)	63, 216, 234, 356, 430, 431
LweI	GCATC	3 cut(s)	7, 61, 449
MaeI	CTAG	1 cut(s)	296
MalI	GATC	2 cut(s)	173, 201
MboI	GATC	2 cut(s)	171, 199
MboII	GAAGA	3 cut(s)	98, 247, 264
MflI	RGATCY	1 cut(s)	199
MhlI	GDGCHC	3 cut(s)	93, 153, 325
MluCI	AATT	3 cut(s)	21, 282, 388
MlyI	GAGTC	1 cut(s)	254
MnlI	CCTC	4 cut(s)	184, 190, 298, 360
MroI	TCCGGA	1 cut(s)	202
MseI	TTAA	1 cut(s)	129
MslI	CAYNNNNRTG	1 cut(s)	266
MspA1I	CMGCKG	1 cut(s)	160
MspI	CCGG	2 cut(s)	203, 418
MspR9I	CCNGG	1 cut(s)	419
Mva1269I	GAATGC	1 cut(s)	212
MwoI	GCNNNNNNNGC	3 cut(s)	109, 157, 314
NciI	CCSGG	1 cut(s)	419
NcoI	CCATGG	1 cut(s)	393
NdeII	GATC	2 cut(s)	171, 199
NlaIII	CATG	6 cut(s)	97, 271, 293, 321, 350, 397
NlaIV	GGNNCC	1 cut(s)	201
NspI	RCATGY	1 cut(s)	350
NspV	TTCGAA	1 cut(s)	84
PagI	TCATGA	1 cut(s)	267
PciSI	GCTCTTC	1 cut(s)	81
PctI	GAATGC	1 cut(s)	212
PflFI	GACNNNGTC	1 cut(s)	263
PleI	GAGTC	1 cut(s)	253
PpsI	GAGTC	1 cut(s)	253
Psp124BI	GAGCTC	1 cut(s)	93
PspN4I	GGNNCC	1 cut(s)	201
PstNI	CAGNNNCTG	1 cut(s)	248
PsuI	RGATCY	1 cut(s)	199
PsyI	GACNNNGTC	1 cut(s)	263
RseI	CAYNNNNRTG	1 cut(s)	266
SacI	GAGCTC	1 cut(s)	93
SapI	GCTCTTC	1 cut(s)	81
SaqAI	TTAA	1 cut(s)	129
Sau3AI	GATC	2 cut(s)	171, 199
SchI	GAGTC	1 cut(s)	254
ScrFI	CCNGG	1 cut(s)	419
SduI	GDGCHC	3 cut(s)	93, 153, 325
SetI	ASST	5 cut(s)	82, 93, 119, 371, 427
SfaNI	GCATC	3 cut(s)	7, 61, 449
SfuI	TTCGAA	1 cut(s)	84
SmiMI	CAYNNNNRTG	1 cut(s)	266
SpeI	ACTAGT	1 cut(s)	295
Sse9I	AATT	3 cut(s)	21, 282, 388
SsiI	CCGC	2 cut(s)	153, 158
SspMI	CTAG	1 cut(s)	296
SstI	GAGCTC	1 cut(s)	93
StyD4I	CCNGG	1 cut(s)	417
StyI	CCWWGG	1 cut(s)	393
TaqI	TCGA	1 cut(s)	84
TasI	AATT	3 cut(s)	21, 282, 388
Tru1I	TTAA	1 cut(s)	129
Tru9I	TTAA	1 cut(s)	129
TscAI	CASTG	2 cut(s)	15, 217
TspDTI	ATGAA	5 cut(s)	77, 237, 294, 306, 417
TspGWI	ACGGA	1 cut(s)	420
TspRI	CASTG	2 cut(s)	15, 217
Tth111I	GACNNNGTC	1 cut(s)	263
XapI	RAATTY	1 cut(s)	388
XceI	RCATGY	1 cut(s)	350
XspI	CTAG	1 cut(s)	296

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.