MD14G1074200.v1.1

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0018886
Physical Location & Seq
Forward (+)
8236010 .. 8236561
552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1074200.v1.1.491

Sequence Viewer

Length: 552 bp
ATGAAAGTGCATAAAACAGTACATCAACTGAAGAAGCCTACACTCGCGATCATGTCTAGAAACGGATCGAGATCAATAATGAATGAGAACTTCTTGGTGAAAATGGCTGTGAAAATAGGGTTTCACGTCGAAATTTTGAGGCCGAAGCGCACCACAGAACTAGCGAAGATTTATCGGGTCCTGAACTCAAGCGATGTTATGATAGGAGTTCATGGAGCTGCAATGACACATTTTCTTTTCATGAGGCCTGGCTCAGTTTTTATCCAAATTATTCCAATCGTAACTGCATGGGCAGCAGAGGAATATTATGGTGCACCTGCAAAGAAGCTTGGTTTAAAGTACATTGGCTATGAAATTCTTACCACAGAAAGCTCACTGTATGATAAATATGAATTAGATGATCCCGTTCTTGCAGATCCAACTAAAGTGACAGAACAAGGATGGCAAATCACAAAGAAGATATATCTGCAATGTCAAACTGTGAGATTGGATCTCATAAGGTTTAGGAAGCAGTTGGTTGAAGCTTATGATTACATCATTGATAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

184

Amino Acids

21.09

Weight (kDa)

9.5

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 8 - 88 4e-13 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018886)

Species Orthologous Gene IDs
malus_domestica MD14G1074200.v1.1
pyrus_communis pycom14g06330
rosa_chinensis RchiOBHm_Chr3g0483211
rosa_laevigata RLG00000023296 RLG00000023298
rosa_multiflora Rmu_co8194502.1_g000001 Rmu_sc0007550.1_g000001
rosa_roxburghii Rroxscaffold_6G00398540
rosa_samantha Rh3BG290200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
Acc36I ACCTGC 1 cut(s) 325
AccII CGCG 1 cut(s) 47
AclWI GGATC 4 cut(s) 73, 395, 410, 498
AcsI RAATTY 2 cut(s) 132, 354
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 2 cut(s) 21, 341
AgsI TTSAA 1 cut(s) 521
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 4 cut(s) 218, 328, 372, 524
AluI AGCT 4 cut(s) 218, 328, 372, 524
Alw21I GWGCWC 1 cut(s) 316
Alw44I GTGCAC 1 cut(s) 312
AlwI GGATC 4 cut(s) 73, 395, 410, 498
AoxI GGCC 2 cut(s) 140, 245
ApaLI GTGCAC 1 cut(s) 312
ApeKI GCWGC 2 cut(s) 218, 293
ApoI RAATTY 2 cut(s) 132, 354
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 1 cut(s) 178
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 1 cut(s) 178
BaeGI GKGCMC 1 cut(s) 316
Bbv12I GWGCWC 1 cut(s) 316
BbvI GCAGC 2 cut(s) 205, 305
BccI CCATC 1 cut(s) 435
BciT130I CCWGG 1 cut(s) 249
BfaI CTAG 2 cut(s) 57, 161
BfuAI ACCTGC 1 cut(s) 325
BisI GCNGC 2 cut(s) 219, 294
BlsI GCNGC 2 cut(s) 220, 295
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 1 cut(s) 178
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 178
BmiI GGNNCC 1 cut(s) 179
BmrFI CCNGG 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 172
BsaBI GATNNNNATC 1 cut(s) 70
Bse3DI GCAATG 2 cut(s) 228, 476
Bse8I GATNNNNATC 1 cut(s) 70
BseBI CCWGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 446
BseJI GATNNNNATC 1 cut(s) 70
BseMI GCAATG 2 cut(s) 228, 476
BseMII CTCAG 1 cut(s) 267
BseSI GKGCMC 1 cut(s) 316
BseXI GCAGC 2 cut(s) 205, 305
Bsh1236I CGCG 1 cut(s) 47
BshFI GGCC 2 cut(s) 142, 247
BsiHKAI GWGCWC 1 cut(s) 316
BsnI GGCC 2 cut(s) 142, 247
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 6 cut(s) 48, 65, 71, 400, 415, 490
Bsp68I TCGCGA 1 cut(s) 47
BspANI GGCC 2 cut(s) 142, 247
BspCNI CTCAG 1 cut(s) 266
BspFNI CGCG 1 cut(s) 47
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 179
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 4 cut(s) 73, 395, 410, 498
BsrDI GCAATG 2 cut(s) 228, 476
BssMI GATC 6 cut(s) 48, 65, 71, 400, 415, 490
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 3 cut(s) 19, 378, 481
BstDEI CTNAG 1 cut(s) 253
BstF5I GGATG 1 cut(s) 446
BstFNI CGCG 1 cut(s) 47
BstHHI GCGC 1 cut(s) 150
BstKTI GATC 6 cut(s) 51, 68, 74, 403, 418, 493
BstMBI GATC 6 cut(s) 48, 65, 71, 400, 415, 490
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 247
BstSLI GKGCMC 1 cut(s) 316
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 2 cut(s) 205, 305
BstX2I RGATCY 2 cut(s) 415, 490
BstYI RGATCY 2 cut(s) 415, 490
BsuRI GGCC 2 cut(s) 142, 247
BtgZI GCGATG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 1 cut(s) 446
BtsIMutI CAGTG 1 cut(s) 374
BtuMI TCGCGA 1 cut(s) 47
BveI ACCTGC 1 cut(s) 325
CciI TCATGA 1 cut(s) 240
CfoI GCGC 1 cut(s) 150
Cfr13I GGNCC 1 cut(s) 178
Csp6I GTAC 2 cut(s) 20, 340
CviAII CATG 4 cut(s) 52, 212, 241, 288
CviQI GTAC 2 cut(s) 20, 340
DdeI CTNAG 1 cut(s) 253
DpnI GATC 6 cut(s) 50, 67, 73, 402, 417, 492
DpnII GATC 6 cut(s) 48, 65, 71, 400, 415, 490
DraI TTTAAA 1 cut(s) 336
Eco147I AGGCCT 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 247
FaeI CATG 4 cut(s) 55, 215, 244, 291
FatI CATG 4 cut(s) 51, 211, 240, 287
Fnu4HI GCNGC 2 cut(s) 219, 294
FokI GGATG 1 cut(s) 453
Fsp4HI GCNGC 2 cut(s) 219, 294
FspBI CTAG 2 cut(s) 57, 161
GlaI GCGC 1 cut(s) 149
GluI GCNGC 2 cut(s) 219, 294
HaeIII GGCC 2 cut(s) 142, 247
HhaI GCGC 1 cut(s) 150
Hin1II CATG 4 cut(s) 55, 215, 244, 291
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 326, 522
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 314
Hpy188III TCNNGA 5 cut(s) 46, 57, 69, 181, 241
Hpy8I GTNNAC 1 cut(s) 314
Hpy99I CGWCG 1 cut(s) 131
HpyCH4III ACNGT 3 cut(s) 19, 378, 481
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 7 cut(s) 10, 221, 287, 314, 320, 413, 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 1 cut(s) 253
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 4 cut(s) 55, 215, 244, 291
HspAI GCGC 1 cut(s) 148
Kzo9I GATC 6 cut(s) 48, 65, 71, 400, 415, 490
LmnI GCTCC 1 cut(s) 215
LpnPI CCDG 4 cut(s) 194, 234, 261, 330
Lsp1109I GCAGC 2 cut(s) 205, 305
MaeI CTAG 2 cut(s) 57, 161
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 280, 427
MalI GATC 6 cut(s) 50, 67, 73, 402, 417, 492
MboI GATC 6 cut(s) 48, 65, 71, 400, 415, 490
MboII GAAGA 3 cut(s) 43, 178, 469
MflI RGATCY 2 cut(s) 415, 490
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 4 cut(s) 132, 267, 354, 392
MmeI TCCRAC 1 cut(s) 443
MnlI CCTC 3 cut(s) 132, 237, 292
MseI TTAA 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 1 cut(s) 293
NdeII GATC 6 cut(s) 48, 65, 71, 400, 415, 490
NlaIII CATG 4 cut(s) 55, 215, 244, 291
NlaIV GGNNCC 1 cut(s) 179
NmuCI GTSAC 1 cut(s) 427
NruI TCGCGA 1 cut(s) 47
PagI TCATGA 1 cut(s) 240
PaqCI CACCTGC 1 cut(s) 325
PceI AGGCCT 1 cut(s) 247
PkrI GCNGC 2 cut(s) 220, 295
PpuMI RGGWCCY 1 cut(s) 178
Psp5II RGGWCCY 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 179
PspPI GGNCC 1 cut(s) 178
PspPPI RGGWCCY 1 cut(s) 178
PsuI RGATCY 2 cut(s) 415, 490
RruI TCGCGA 1 cut(s) 47
RsaI GTAC 2 cut(s) 21, 341
RsaNI GTAC 2 cut(s) 20, 340
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 2 cut(s) 219, 294
Sau3AI GATC 6 cut(s) 48, 65, 71, 400, 415, 490
Sau96I GGNCC 1 cut(s) 178
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 1 cut(s) 316
SetI ASST 7 cut(s) 129, 220, 319, 330, 374, 503, 526
SinI GGWCC 1 cut(s) 178
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
Sse9I AATT 4 cut(s) 132, 267, 354, 392
SseBI AGGCCT 1 cut(s) 247
SspI AATATT 1 cut(s) 305
SspMI CTAG 2 cut(s) 57, 161
StuI AGGCCT 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 3 cut(s) 19, 378, 481
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 68, 129
TasI AATT 4 cut(s) 132, 267, 354, 392
TatI WGTACW 2 cut(s) 19, 339
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 1 cut(s) 381
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 2 cut(s) 218, 293
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 6 cut(s) 17, 95, 200, 229, 366, 405
TspGWI ACGGA 1 cut(s) 78
TspRI CASTG 1 cut(s) 381
VneI GTGCAC 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 178
XapI RAATTY 2 cut(s) 132, 354
XbaI TCTAGA 1 cut(s) 56
XspI CTAG 2 cut(s) 57, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.