RLG00000023296

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018886
Physical Location & Seq
Forward (+)
21298125 .. 21298701
577 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023296

Sequence Viewer

Length: 480 bp
ATGAAAGCTCATTGCTTCCCCGAGGCCAATGTTGGTCTGAGAATTCATGATGAGCTCAGTAACAAGAAGCACAAGATCGACAAACTTTCTGCTTCTGTTTCTCCAACATGGAACATATCAATAAAAAGCAAGGCAGAAGTGGAAGCACCAAAATTGAGGAATTCAAAATTGGTTATCATATCTAGAAATGGATCAAGAGCGACAACTAATGAGAACTTCTTGGTGAAGATGGTTGAGAAAATTGGTTTTCAAGTTCAAGTTTTGAGACCCGAGCCAACAACTGAATCAGCAAAGATTTACTGGGATCTAAATTCAAGTGATGTCATGATTGGAGTTCGTGGTGCTGCCATCACACAACTTCTGTTTATGAAGCCTGGATGGGGAGCTGAGGCCTATTATGGAGGATCTGCAAGGAAGCTTGGCCTAAAGTACACTGGCTATCACATTCTTACCAAGGAGAGCTCATTGTATGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

160

Amino Acids

17.76

Weight (kDa)

9.65

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 38 - 126 3.2e-11 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018886)

Species Orthologous Gene IDs
malus_domestica MD14G1074200.v1.1
pyrus_communis pycom14g06330
rosa_chinensis RchiOBHm_Chr3g0483211
rosa_laevigata RLG00000023296 RLG00000023298
rosa_multiflora Rmu_co8194502.1_g000001 Rmu_sc0007550.1_g000001
rosa_roxburghii Rroxscaffold_6G00398540
rosa_samantha Rh3BG290200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 199, 312, 412
AcsI RAATTY 3 cut(s) 42, 160, 310
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 380
AgsI TTSAA 4 cut(s) 165, 251, 257, 315
AjnI CCWGG 1 cut(s) 373
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 5 cut(s) 8, 55, 386, 418, 462
AluI AGCT 5 cut(s) 8, 55, 386, 418, 462
Alw21I GWGCWC 2 cut(s) 57, 464
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 3 cut(s) 199, 312, 412
Ama87I CYCGRG 2 cut(s) 20, 269
AoxI GGCC 3 cut(s) 24, 390, 421
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 3 cut(s) 42, 160, 310
AsuHPI GGTGA 1 cut(s) 235
AvaI CYCGRG 2 cut(s) 20, 269
BanII GRGCYC 2 cut(s) 57, 464
Bbv12I GWGCWC 2 cut(s) 57, 464
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 331
BccI CCATC 3 cut(s) 223, 356, 372
BciT130I CCWGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 1 cut(s) 183
BisI GCNGC 1 cut(s) 345
BlsI GCNGC 1 cut(s) 346
Bme1390I CCNGG 1 cut(s) 375
BmeT110I CYCGRG 2 cut(s) 20, 269
BmrFI CCNGG 1 cut(s) 375
BmrI ACTGGG 1 cut(s) 310
BmuI ACTGGG 1 cut(s) 310
Bpu10I CCTNAGC 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 2 cut(s) 21, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 380
Bse1I ACTGG 2 cut(s) 305, 439
Bse3DI GCAATG 1 cut(s) 10
BseBI CCWGG 1 cut(s) 375
BseDI CCNNGG 2 cut(s) 21, 453
BseGI GGATG 1 cut(s) 383
BseLI CCNNNNNNNGG 1 cut(s) 380
BseMI GCAATG 1 cut(s) 10
BseMII CTCAG 3 cut(s) 29, 70, 378
BseNI ACTGG 2 cut(s) 305, 439
BseXI GCAGC 1 cut(s) 331
BshFI GGCC 3 cut(s) 26, 392, 423
BsiHKAI GWGCWC 2 cut(s) 57, 464
BsiHKCI CYCGRG 2 cut(s) 20, 269
BslI CCNNNNNNNGG 1 cut(s) 380
BsmAI GTCTC 1 cut(s) 259
BsnI GGCC 3 cut(s) 26, 392, 423
Bso31I GGTCTC 1 cut(s) 259
BsoBI CYCGRG 2 cut(s) 20, 269
Bsp1286I GDGCHC 2 cut(s) 57, 464
Bsp143I GATC 4 cut(s) 75, 191, 304, 404
BspANI GGCC 3 cut(s) 26, 392, 423
BspCNI CTCAG 3 cut(s) 30, 69, 379
BspHI TCATGA 2 cut(s) 46, 324
BspPI GGATC 3 cut(s) 199, 312, 412
BspTNI GGTCTC 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 10
BsrI ACTGG 2 cut(s) 305, 439
BssECI CCNNGG 2 cut(s) 21, 453
BssMI GATC 4 cut(s) 75, 191, 304, 404
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 375
BstDEI CTNAG 3 cut(s) 38, 56, 387
BstF5I GGATG 1 cut(s) 383
BstKTI GATC 4 cut(s) 78, 194, 307, 407
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 4 cut(s) 75, 191, 304, 404
BstNI CCWGG 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 373
BstV1I GCAGC 1 cut(s) 331
BstX2I RGATCY 2 cut(s) 304, 404
BstYI RGATCY 2 cut(s) 304, 404
BsuRI GGCC 3 cut(s) 26, 392, 423
BtsCI GGATG 1 cut(s) 383
BtsIMutI CAGTG 1 cut(s) 432
CciI TCATGA 2 cut(s) 46, 324
Csp6I GTAC 1 cut(s) 430
CviAII CATG 3 cut(s) 47, 108, 325
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 3 cut(s) 38, 56, 387
DpnI GATC 4 cut(s) 77, 193, 306, 406
DpnII GATC 4 cut(s) 75, 191, 304, 404
Ecl136II GAGCTC 2 cut(s) 55, 462
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 1 cut(s) 392
Eco24I GRGCYC 2 cut(s) 57, 464
Eco31I GGTCTC 1 cut(s) 259
Eco53kI GAGCTC 2 cut(s) 55, 462
Eco88I CYCGRG 2 cut(s) 20, 269
EcoICRI GAGCTC 2 cut(s) 55, 462
EcoRI GAATTC 2 cut(s) 42, 160
EcoRII CCWGG 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 2 cut(s) 57, 464
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 3 cut(s) 50, 111, 328
FaiI YATR 8 cut(s) 48, 109, 116, 179, 326, 368, 399, 471
FatI CATG 3 cut(s) 46, 107, 324
Fnu4HI GCNGC 1 cut(s) 345
FokI GGATG 1 cut(s) 390
FriOI GRGCYC 2 cut(s) 57, 464
Fsp4HI GCNGC 1 cut(s) 345
FspBI CTAG 1 cut(s) 183
GluI GCNGC 1 cut(s) 345
HaeIII GGCC 3 cut(s) 26, 392, 423
Hin1II CATG 3 cut(s) 50, 111, 328
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 1 cut(s) 284
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 432
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 4 cut(s) 47, 183, 195, 325
Hpy8I GTNNAC 1 cut(s) 432
HpyCH4V TGCA 1 cut(s) 410
HpyF3I CTNAG 3 cut(s) 38, 56, 387
Hsp92II CATG 3 cut(s) 50, 111, 328
Kzo9I GATC 4 cut(s) 75, 191, 304, 404
LmnI GCTCC 1 cut(s) 383
LpnPI CCDG 4 cut(s) 286, 360, 387, 420
Lsp1109I GCAGC 1 cut(s) 331
MaeI CTAG 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 59
MalI GATC 4 cut(s) 77, 193, 306, 406
MboI GATC 4 cut(s) 75, 191, 304, 404
MboII GAAGA 1 cut(s) 238
MflI RGATCY 2 cut(s) 304, 404
MhlI GDGCHC 2 cut(s) 57, 464
MluCI AATT 6 cut(s) 42, 152, 160, 167, 240, 310
MmeI TCCRAC 1 cut(s) 128
MnlI CCTC 4 cut(s) 16, 150, 382, 395
MspR9I CCNGG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 375
NdeII GATC 4 cut(s) 75, 191, 304, 404
NlaIII CATG 3 cut(s) 50, 111, 328
PagI TCATGA 2 cut(s) 46, 324
PceI AGGCCT 1 cut(s) 392
PfeI GAWTC 1 cut(s) 284
PkrI GCNGC 1 cut(s) 346
Psp124BI GAGCTC 2 cut(s) 57, 464
Psp6I CCWGG 1 cut(s) 373
PspGI CCWGG 1 cut(s) 373
PsuI RGATCY 2 cut(s) 304, 404
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
SacI GAGCTC 2 cut(s) 57, 464
SatI GCNGC 1 cut(s) 345
Sau3AI GATC 4 cut(s) 75, 191, 304, 404
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 2 cut(s) 57, 464
SetI ASST 5 cut(s) 10, 57, 388, 420, 464
Sse9I AATT 6 cut(s) 42, 152, 160, 167, 240, 310
SseBI AGGCCT 1 cut(s) 392
SspMI CTAG 1 cut(s) 183
SstI GAGCTC 2 cut(s) 57, 464
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 1 cut(s) 453
TaqI TCGA 1 cut(s) 78
TasI AATT 6 cut(s) 42, 152, 160, 167, 240, 310
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 1 cut(s) 284
TscAI CASTG 1 cut(s) 439
TseI GCWGC 1 cut(s) 344
TspDTI ATGAA 3 cut(s) 17, 35, 383
TspRI CASTG 1 cut(s) 439
XapI RAATTY 3 cut(s) 42, 160, 310
XbaI TCTAGA 1 cut(s) 182
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.