RLG00000023298

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018886
Physical Location & Seq
Forward (+)
21334163 .. 21335714
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023298

Sequence Viewer

Length: 924 bp
ATGGTACACTACTATATATGCAATATCGACAGGGAACGAAAGGTTCACATCAGCCTCAAGAAGAGGACGAAGAATTCACCGGAAGTAGACCATGAAGGCCTTGCTCCTGATATGGATATCCACAGACAAACCAAGCTTCATGAAAAATCTATCAGTCGTTATAGCGAGGAAAATGGACAGGGTGACAATGAGGACGAAATCCAACATGAAAAGATCAAACCATATAATCGAAAATGGGAAGCAAATACCACGGCAACCATTACTGAATTACACCTTATTGCAAAGACTGATAGTACTCCTGCACACACCATCCATGTCAAAATGATGCTCCAGCACTGGTTTTTTTCAATTGCAGGCTATACTGGTAACGTCTACCATGAATTCAATGATGGAATTTTGCCATTGTCCATTACTTTACAGCACTTCAACAAGGTTGTGTTCGTAATTGTTGATTTTCATGATTGGTGGGCCACAAAATATGGAGACATTCTTTCCCAACTATCAGATTATCCTCCTATAGATTTTGTTGGAGATATGAAAACTCATTGCTTCCCTGAGGCCAATGTTGGTCTGAGAATTCCTGATGAGCTCAGTAACAAGAAGCACAAGATCGACAAACTTTCTGCTTCTGTTTCTCCAACATGGAACATATCAATAAAAAGCAAGGCAGAAGTGGAAGCACCAAAATTGAGGAATTCAAAATTGGTTATCATATCTAGAAATGGATCAAGAGCGACAACTAATGAGAACTTCTTTGTGAAGATGGTTGAGAAAATTGGTTTTCAAGTTCAAGTTTTGAGACCCGAGCCAACAACTGAATCAGCAAAGATTTACTGGGATCTAAATTCAAGTGATGTCATGATTGGAGTTCGTGGTGCTGCCATCACACAACTTCTGTTTATGAAAGCCTGGATGGGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

308

Amino Acids

35.26

Weight (kDa)

7.28

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 216 - 303 3.4e-09 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018886)

Species Orthologous Gene IDs
malus_domestica MD14G1074200.v1.1
pyrus_communis pycom14g06330
rosa_chinensis RchiOBHm_Chr3g0483211
rosa_laevigata RLG00000023296 RLG00000023298
rosa_multiflora Rmu_co8194502.1_g000001 Rmu_sc0007550.1_g000001
rosa_roxburghii Rroxscaffold_6G00398540
rosa_samantha Rh3BG290200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 87, 372
AclWI GGATC 2 cut(s) 733, 846
AcsI RAATTY 6 cut(s) 73, 380, 393, 576, 694, 844
AfaI GTAC 2 cut(s) 6, 295
AfiI CCNNNNNNNGG 1 cut(s) 915
AgsI TTSAA 7 cut(s) 348, 385, 427, 699, 785, 791, 849
AjnI CCWGG 1 cut(s) 908
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AluBI AGCT 3 cut(s) 136, 589, 921
AluI AGCT 3 cut(s) 136, 589, 921
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 2 cut(s) 477, 793
AlwI GGATC 2 cut(s) 733, 846
Ama87I CYCGRG 1 cut(s) 803
AoxI GGCC 3 cut(s) 97, 468, 558
ApeKI GCWGC 1 cut(s) 878
ApoI RAATTY 6 cut(s) 73, 380, 393, 576, 694, 844
AspS9I GGNCC 1 cut(s) 468
AsuHPI GGTGA 2 cut(s) 69, 194
AvaI CYCGRG 1 cut(s) 803
AxyI CCTNAGG 1 cut(s) 555
BanII GRGCYC 1 cut(s) 591
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 865
BccI CCATC 5 cut(s) 317, 383, 757, 890, 907
BceAI ACGGC 1 cut(s) 267
BciT130I CCWGG 1 cut(s) 910
BcoDI GTCTC 2 cut(s) 477, 793
BfaI CTAG 1 cut(s) 717
BfmI CTRYAG 1 cut(s) 516
BisI GCNGC 1 cut(s) 879
BlsI GCNGC 1 cut(s) 880
BmcAI AGTACT 1 cut(s) 295
Bme1390I CCNGG 1 cut(s) 910
BmeT110I CYCGRG 1 cut(s) 803
BmgT120I GGNCC 1 cut(s) 468
BmrFI CCNGG 1 cut(s) 910
BmrI ACTGGG 1 cut(s) 844
BmsI GCATC 1 cut(s) 315
BmuI ACTGGG 1 cut(s) 844
BpmI CTGGAG 1 cut(s) 314
BpuEI CTTGAG 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 793
BsaJI CCNNGG 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 1 cut(s) 915
Bse1I ACTGG 3 cut(s) 341, 367, 839
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 1 cut(s) 544
BseBI CCWGG 1 cut(s) 910
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 2 cut(s) 309, 918
BseLI CCNNNNNNNGG 1 cut(s) 915
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 3 cut(s) 546, 563, 604
BseNI ACTGG 3 cut(s) 341, 367, 839
BseXI GCAGC 1 cut(s) 865
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 3 cut(s) 99, 470, 560
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 803
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 915
BsmAI GTCTC 2 cut(s) 477, 793
BsnI GGCC 3 cut(s) 99, 470, 560
Bso31I GGTCTC 1 cut(s) 793
BsoBI CYCGRG 1 cut(s) 803
Bsp1286I GDGCHC 1 cut(s) 591
Bsp143I GATC 4 cut(s) 213, 609, 725, 838
BspANI GGCC 3 cut(s) 99, 470, 560
BspCNI CTCAG 3 cut(s) 547, 564, 603
BspHI TCATGA 3 cut(s) 139, 457, 858
BspPI GGATC 2 cut(s) 733, 846
BspTNI GGTCTC 1 cut(s) 793
BsrDI GCAATG 1 cut(s) 544
BsrI ACTGG 3 cut(s) 341, 367, 839
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 4 cut(s) 213, 609, 725, 838
Bst2UI CCWGG 1 cut(s) 910
Bst6I CTCTTC 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 3 cut(s) 555, 572, 590
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 2 cut(s) 309, 918
BstKTI GATC 4 cut(s) 216, 612, 728, 841
BstMAI GTCTC 2 cut(s) 477, 793
BstMBI GATC 4 cut(s) 213, 609, 725, 838
BstNI CCWGG 1 cut(s) 910
BstSCI CCNGG 1 cut(s) 908
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 1 cut(s) 865
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
Bsu36I CCTNAGG 1 cut(s) 555
BsuRI GGCC 3 cut(s) 99, 470, 560
BtgI CCRYGG 1 cut(s) 249
BtsCI GGATG 2 cut(s) 309, 918
BtsIMutI CAGTG 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 355
CciI TCATGA 3 cut(s) 139, 457, 858
Cfr13I GGNCC 1 cut(s) 468
Csp6I GTAC 2 cut(s) 5, 294
CviAII CATG 8 cut(s) 92, 140, 206, 314, 377, 458, 642, 859
CviQI GTAC 2 cut(s) 5, 294
DdeI CTNAG 3 cut(s) 555, 572, 590
DpnI GATC 4 cut(s) 215, 611, 727, 840
DpnII GATC 4 cut(s) 213, 609, 725, 838
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Ecl136II GAGCTC 1 cut(s) 589
Eco147I AGGCCT 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 591
Eco31I GGTCTC 1 cut(s) 793
Eco32I GATATC 1 cut(s) 118
Eco53kI GAGCTC 1 cut(s) 589
Eco81I CCTNAGG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 803
EcoICRI GAGCTC 1 cut(s) 589
EcoRI GAATTC 4 cut(s) 73, 380, 576, 694
EcoRII CCWGG 1 cut(s) 908
EcoRV GATATC 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 591
FaeI CATG 8 cut(s) 95, 143, 209, 317, 380, 461, 645, 862
FatI CATG 8 cut(s) 91, 139, 205, 313, 376, 457, 641, 858
FblI GTMKAC 2 cut(s) 87, 372
Fnu4HI GCNGC 1 cut(s) 879
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 1 cut(s) 591
Fsp4HI GCNGC 1 cut(s) 879
FspBI CTAG 1 cut(s) 717
GluI GCNGC 1 cut(s) 879
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 3 cut(s) 99, 470, 560
HapII CCGG 1 cut(s) 80
Hin1II CATG 8 cut(s) 95, 143, 209, 317, 380, 461, 645, 862
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 1 cut(s) 818
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 69, 194
Hpy166II GTNNAC 4 cut(s) 7, 46, 88, 373
Hpy188I TCNGA 2 cut(s) 505, 573
Hpy188III TCNNGA 8 cut(s) 58, 107, 140, 458, 581, 717, 729, 859
Hpy8I GTNNAC 4 cut(s) 7, 46, 88, 373
HpyAV CCTTC 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 4 cut(s) 21, 281, 302, 353
HpyF3I CTNAG 3 cut(s) 555, 572, 590
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 8 cut(s) 95, 143, 209, 317, 380, 461, 645, 862
Kzo9I GATC 4 cut(s) 213, 609, 725, 838
LmnI GCTCC 3 cut(s) 109, 333, 918
Lsp1109I GCAGC 1 cut(s) 865
LweI GCATC 1 cut(s) 315
MaeI CTAG 1 cut(s) 717
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 3 cut(s) 182, 365, 593
MalI GATC 4 cut(s) 215, 611, 727, 840
MboI GATC 4 cut(s) 213, 609, 725, 838
MboII GAAGA 3 cut(s) 73, 82, 772
MfeI CAATTG 1 cut(s) 348
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 1 cut(s) 591
MmeI TCCRAC 3 cut(s) 226, 508, 662
MnlI CCTC 7 cut(s) 57, 65, 160, 184, 522, 550, 684
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 910
MunI CAATTG 1 cut(s) 348
MvaI CCWGG 1 cut(s) 910
NdeII GATC 4 cut(s) 213, 609, 725, 838
NlaIII CATG 8 cut(s) 95, 143, 209, 317, 380, 461, 645, 862
NmuCI GTSAC 1 cut(s) 182
PagI TCATGA 3 cut(s) 139, 457, 858
PceI AGGCCT 1 cut(s) 99
PfeI GAWTC 1 cut(s) 818
PkrI GCNGC 1 cut(s) 880
Psp124BI GAGCTC 1 cut(s) 591
Psp6I CCWGG 1 cut(s) 908
PspGI CCWGG 1 cut(s) 908
PspPI GGNCC 1 cut(s) 468
PsuI RGATCY 1 cut(s) 838
RsaI GTAC 2 cut(s) 6, 295
RsaNI GTAC 2 cut(s) 5, 294
SacI GAGCTC 1 cut(s) 591
SatI GCNGC 1 cut(s) 879
Sau3AI GATC 4 cut(s) 213, 609, 725, 838
Sau96I GGNCC 1 cut(s) 468
ScaI AGTACT 1 cut(s) 295
ScrFI CCNGG 1 cut(s) 910
SduI GDGCHC 1 cut(s) 591
SetI ASST 7 cut(s) 45, 138, 276, 372, 435, 591, 923
SfaNI GCATC 1 cut(s) 315
SfcI CTRYAG 1 cut(s) 516
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SseBI AGGCCT 1 cut(s) 99
SspMI CTAG 1 cut(s) 717
SstI GAGCTC 1 cut(s) 591
StuI AGGCCT 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 908
TaiI ACGT 1 cut(s) 372
TaqI TCGA 3 cut(s) 27, 229, 612
TatI WGTACW 1 cut(s) 293
TfiI GAWTC 1 cut(s) 818
TscAI CASTG 1 cut(s) 341
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 1 cut(s) 878
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 8 cut(s) 108, 128, 156, 222, 393, 446, 551, 917
TspRI CASTG 1 cut(s) 341
XapI RAATTY 6 cut(s) 73, 380, 393, 576, 694, 844
XbaI TCTAGA 1 cut(s) 716
XmiI GTMKAC 2 cut(s) 87, 372
XspI CTAG 1 cut(s) 717
ZrmI AGTACT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.