Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0009540

Physical Location & Seq

Genomic Coordinates Reverse (-)

8291637 .. 8299224

Gene Structure 7588 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1074400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3351 bp

ATGTTATTGAATTCTCTCTCTCTGAAAGAAGAAATGGAGGACAGTGCAAGAACTGGATTGCATGATTTTGCAAGGGCTTCAAGAAAAGCAGAAGAAGCAGCTTGGAGGCGATTCCAAGCCGTTGAATGGCTTGAAAGCCTGGTAGGTCCACTCGGCGTACCAAAACAGCCATCGGAGAGAGACTTCATTTCTTGCTTGAGAAATGGTCTGATCCTGTGCAATGCTATCAACAAGATTCAACCAGGAGCAGTGCCCAAGGTTGTGGAGAGCCAAATGCCTTTGCAATCGTTAAGTCGGGAATCTCAAGCATTGCCTGCCTATCAGTACTTTGAGAATGTCCGTAACTTTCTGGTCGCTGTGGAAGAGTTGATGCTGCCTGCTTTTGAAGCTTCCGACCTCGAAAGGGAAGCAATGGAGGCAGGGTCAGCAGCCAAGGTTGTCGACTGTGTTTTATCCCTTAAATCGTATTATGAGTGGAAGCAGATGAGCAATGGGAATGGATTTACTAAATATGTGAAATCTCCCCTAGTTATGGTTTCTGCCAATAGACTGCATTCAAGGGCCTCAACTGTAGTCCCATCAGATTCTTGCAGGCACTTGGATATGTCTGCCGTATGTGAGAGACAACCACCTGTAGAGGGTGACAACCAGAAAGTTGAAGATGTGGATTCCGTAGAATCTGTTGTCAAGTTACTTTTTGACTCTATGGTTGACGTAAAGGAAAACATTGATGACAACGTTCTTGCTTCCTACCATAGTGGAGATGTGAATGCAGTCAGCCTCTTACGAAGAATCATGAAAAGTTGTTTGGAGCAACATCTACAGAAGAAGTTTCCAGAGTTAAACTCTAAATTAGAAGAACCTTTGAAAGAAAAAAGGAGATCAACTGGCCTTTCAACAGCTATGCCTCTAGAGGAGTCATCTGCTCCTGGAAATTCCGGATGCTGCAGAGATTGTTTAAGAAAGGGTAACTGCAACCACAGACTTCTATTCCAAACGCAAGAGAAAGAACTTGTGGAACTCAAGGCCTTGTGGTTAAGTGCAAAACGGGACTTTGAAGACTTACAGACACAACTGAATAGTGATTTGAAACACTTGGTTAATCAGGTGCAGGAGCTGTCAACTGCAGCACTCGGGTATTACAAGGTGGTGAAAGAGAATCAAAAGCTATACAACATGGTTCAGGATTTGAAAGGTAATATTCGAGTTTACTGCCGGATTAGGCCTTCCTTCAGTTCTGAATCTGGAAATGTAATAAGTTTCATTGGAGAGGACGGGTCACTTGTGATCTCGGACCCTGCAAAACCCCAAAAGGATGGAAGGAAAGTCTTCCAGTTCAACCGAGTGTTTGGTCCAACTGCTAGGCAAGATGAGGTCTTTAAGGACACTCAACCACTGATCAGGTCTGTGATGGATGGTTACAATGTATGCATTTTTGCTTATGGTCAAACTGGATCCGGTAAAACACATACCATGAGTGGTCCATCAGGAGGGTCAGCTGCGGATATGGGGATTAATTATCTAGCTCTCAGCGATTTGTTCCAGATGTCAAACAAAAGGAAGGATATCATAGATTATGACATTCATGTCCAAATGGTCGAAATTTACAATGAACAAGTACGAGACCTTCTTGCAGAAGATTCATCCACTGCCAAATTAGAGATACGGAGCTGTGCTGGTGATAATGGCTTGAGTATCCCAGGTGCTACAATGCATTCAGTGAAGTCTACTACTGATGTCTTGAACCTCATGAAATTTGGTGAGATGAATCGTATGGTCAGTTCAACCGCAATCAATAGCCGCAGCAGCCGTTCCCATAGTGTCCTAACAGTCCATGTGCATGGAAAAGATACTTCCGGAGGTACTCTACGTAGTTGCCTTCATTTGGTTGACCTTGCAGGAAGCGAAAGAGTGGACAAATCTGAAGTTACAGGAGATAGGCTGAAAGAGGCACAGTATATCAACAAGTCTCTTTCATGTTTAGGAGATGTTATCGCAGCCTTGGCTCAGAAGAATTCTCACATCCCTTACAGAAACAGCAAACTCACACTTCTGTTGCAAGACTCCTTAGGTGGACATGCTAAAACTTTAATGCTTGCTCATGTTAGTCCAGAAGAAGATTCCTTCAGTGAAACTATCAGTACCTTGAAGTTTGCACAAAGGGTTTCCACCGTTGAACTCGGTGCTGCTCATTCAAACAAGGAGAGTGGTGAGGTTATGAAACTCAAAGAACAGATTGAGAGTCTTAAGAAGGCATTAGCAAACAAAGAAGGTCAATGTGTGCCAAGAACAATGACTGAGAGAACTCCCCAACGTCTGAGAAGATTAAGCATTGAAAGTTGCAGCACTGTGAAAACCGAGAAGGCAAAAACCCCTTATATTCCAACTCGTTTGAGGAGATTGAGCTTGGAAGGTCCAAGATCAATTAAGAAGGATAATCTGCATATATCGCATGACATGGGGAAAATCCTAACATCTGAGGCAGTGACAGTGCAGGAATACGGTCAACTTCAAAGTGCAGCGGCAGTCACTACCCCATTGGGCCATTTCAGTAATGAGGATTCCACATTAGAAGTGTTTTGTCCCAAGGCTCCTCAAAGTCCAACCAGCACTACCTACGGGAAGCAAGTGTTGGAACCAGGTAGTAGAACACAAGTTCCTTCCCTTCTAAAACCAACAACACCCGAACCTCGTCCCAGGAATCTTCTAAGTCCAGCAAGTGCTACATATCAGAAGCGAGTGTTGGAACCAGGTAGTAGAACCCAAGTTCCTCCTCCTCAGCGACCAACGACACCTGAGCCTCAGACCAAGGCTCCTCATAGCCCAAAGATGGCTCCTCGCAGCCCAAGGATGGGTAGCTATCAGAAGCAAGGGTTAAAAGCAGATAACAAGACACAAGTTCGTCCCTTTCAGCTACCAACAACACCCGAACCAAAAAAGCATTCCAGAAATGAGGTACAGATTGTCATGCAAAGTAAGGTCACTCTTTCTACAGATTACCTGACACCTAATTTGACCAGTAGCACAAGTGGAAAAGGGTCTCAGATAAGGAAATCCCTGCGGACAATTGGAAAACTGATCAATGGCTCTGAGAAGAGGAACCAGCAAAGTTTGGTGGAAGCACAGTCAAGTGTTAAATGCGCAACCAATATAAATGATGCAAATTCACCGGTAACAAATAGTGCAAGAACTCTGAGGCGGCAATCACTAACAGGCATTCCGCCACCAGGGTATGACAGACGATCATCTCTAGGAGGGAAGCCAACCGAAACTAGTGCAAAGGACAACAGAAATGCCAAAACACCTCCGCCAGTCCGTTCATCAACAAAAAGTAACAAGCGGTGGCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

1117

Amino Acids

123.44

Weight (kDa)

9.2

Isoelectric Point (pI)

54.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	39 - 158	9.6e-12	Calponin homology (CH) domain
Microtub_bd	PF16796	386 - 544	3.3e-42	Microtubule binding
Kinesin	PF00225	406 - 724	1.5e-108	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009540)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10310 AT3G10310
fragaria_vesca	FvH4_6g24140 FvH4_6g24140 FvH4_6g24140 FvH4_6g24140 FvH4_6g24140 FvH4_6g24140 FvH4_6g24140
malus_domestica	MD12G1076000.v1.1 MD14G1074400.v1.1
prunus_persica	Prupe.7G055800_v2.0.a1
pyrus_communis	pycom14g06340
rosa_chinensis	RchiOBHm_Chr3g0483041
rosa_laevigata	RLG00000023304
rosa_multiflora	Rmu_sc0000441.1_g000128
rosa_roxburghii	Rroxscaffold_6G00398680
rosa_rugosa	Rorug03G0204100
rosa_samantha	Rh3AG251900 Rh3CG286100 Rh3DG281700
rosa_wichuraiana	Rw3G022800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1586
Acc16I	TGCGCA	1 cut(s)	3142
AccI	GTMKAC	2 cut(s)	441, 1727
AccIII	TCCGGA	2 cut(s)	938, 1856
AciI	CCGC	9 cut(s)	1502, 1788, 1801, 2522, 3061, 3199, 3221, 3308, 3340
AclI	AACGTT	1 cut(s)	738
AclWI	GGATC	3 cut(s)	205, 1449, 1462
AcsI	RAATTY	6 cut(s)	10, 934, 1602, 1754, 2014, 3163
AcuI	CTGAAG	3 cut(s)	1216, 1944, 2110
AfaI	GTAC	6 cut(s)	159, 326, 1620, 1864, 2143, 2958
AflII	CTTAAG	1 cut(s)	2246
AgeI	ACCGGT	1 cut(s)	3169
AhlI	ACTAGT	1 cut(s)	3272
AjnI	CCWGG	8 cut(s)	138, 241, 928, 1699, 2638, 2696, 2749, 3226
AjuI	GAANNNNNNNTTGG	8 cut(s)	791, 823, 2615, 2647, 2726, 2758, 2926, 2958
AloI	GAACNNNNNNTCC	4 cut(s)	2641, 2673, 2752, 2784
Alw26I	GTCTC	5 cut(s)	174, 616, 1617, 1974, 3045
AlwI	GGATC	3 cut(s)	205, 1449, 1462
AlwNI	CAGNNNCTG	1 cut(s)	1117
Ama87I	CYCGRG	1 cut(s)	1133
Aor13HI	TCCGGA	2 cut(s)	938, 1856
AoxI	GGCC	5 cut(s)	561, 889, 1026, 1223, 2542
ApoI	RAATTY	6 cut(s)	10, 934, 1602, 1754, 2014, 3163
ArsI	GACNNNNNNTTYG	2 cut(s)	2995, 3027
AseI	ATTAAT	1 cut(s)	1515
AsiGI	ACCGGT	1 cut(s)	3169
Asp700I	GAANNNNTTC	1 cut(s)	1328
AspLEI	GCGC	1 cut(s)	3143
AspS9I	GGNCC	7 cut(s)	146, 561, 1294, 1352, 1481, 2414, 2542
AsuHPI	GGTGA	6 cut(s)	653, 1162, 1691, 1772, 2222, 3159
AvaI	CYCGRG	1 cut(s)	1133
AvaII	GGWCC	5 cut(s)	146, 1294, 1352, 1481, 2414
AxyI	CCTNAGG	1 cut(s)	2068
BaeGI	GKGCMC	1 cut(s)	255
BamHI	GGATCC	1 cut(s)	1454
BarI	GAAGNNNNNNTAC	2 cut(s)	1611, 1643
BbsI	GAAGAC	2 cut(s)	1065, 1321
BbvCI	CCTCAGC	1 cut(s)	2778
BccI	CCATC	8 cut(s)	178, 586, 1310, 1405, 1409, 1492, 2824, 2845
BceAI	ACGGC	3 cut(s)	104, 596, 1794
BcgI	CGANNNNNNTGC	4 cut(s)	1194, 1228, 3257, 3291
BciT130I	CCWGG	8 cut(s)	140, 243, 930, 1701, 2640, 2698, 2751, 3228
BciVI	GTATCC	1 cut(s)	1706
BclI	TGATCA	2 cut(s)	1398, 3078
BcoDI	GTCTC	5 cut(s)	174, 616, 1617, 1974, 3045
BcuI	ACTAGT	1 cut(s)	3272
BfaI	CTAG	6 cut(s)	527, 911, 1362, 1523, 3251, 3273
BfmI	CTRYAG	7 cut(s)	570, 633, 821, 946, 1125, 2991, 3347
BfrI	CTTAAG	1 cut(s)	2246
BfuI	GTATCC	1 cut(s)	1706
BmcAI	AGTACT	1 cut(s)	326
Bme1390I	CCNGG	8 cut(s)	140, 243, 930, 1701, 2640, 2698, 2751, 3228
Bme18I	GGWCC	5 cut(s)	146, 1294, 1352, 1481, 2414
BmeT110I	CYCGRG	1 cut(s)	1133
BmgT120I	GGNCC	7 cut(s)	146, 561, 1294, 1352, 1481, 2414, 2542
BmiI	GGNNCC	8 cut(s)	1296, 1456, 2592, 2637, 2748, 2814, 2835, 3101
BmrFI	CCNGG	8 cut(s)	140, 243, 930, 1701, 2640, 2698, 2751, 3228
BmsI	GCATC	3 cut(s)	360, 932, 3148
BpiI	GAAGAC	2 cut(s)	1065, 1321
BplI	GAGNNNNNCTC	2 cut(s)	830, 862
Bpu10I	CCTNAGC	2 cut(s)	2778, 2796
BpuEI	CTTGAG	4 cut(s)	217, 288, 1007, 1711
BsaAI	YACGTR	1 cut(s)	1871
BsaI	GGTCTC	2 cut(s)	1617, 3045
BsaJI	CCNNGG	9 cut(s)	255, 432, 1699, 2001, 2586, 2696, 2808, 2846, 3227
BsaWI	WCCGGW	4 cut(s)	938, 1457, 1856, 3169
BsaXI	ACNNNNNCTCC	4 cut(s)	407, 437, 2797, 2827
Bse118I	RCCGGY	1 cut(s)	3169
Bse1I	ACTGG	6 cut(s)	58, 892, 1333, 1456, 3018, 3311
Bse21I	CCTNAGG	1 cut(s)	2068
Bse3DI	GCAATG	4 cut(s)	226, 308, 417, 496
BseAI	TCCGGA	2 cut(s)	938, 1856
BseBI	CCWGG	8 cut(s)	140, 243, 930, 1701, 2640, 2698, 2751, 3228
BseDI	CCNNGG	9 cut(s)	255, 432, 1699, 2001, 2586, 2696, 2808, 2846, 3227
BseGI	GGATG	6 cut(s)	947, 1321, 1420, 1643, 2022, 2856
BseMI	GCAATG	4 cut(s)	226, 308, 417, 496
BseNI	ACTGG	6 cut(s)	58, 892, 1333, 1456, 3018, 3311
BseRI	GAGGAG	7 cut(s)	929, 2410, 2583, 2763, 2766, 2805, 2826
BseSI	GKGCMC	1 cut(s)	255
BsgI	GTGCAG	3 cut(s)	1130, 2513, 2538
BshFI	GGCC	5 cut(s)	563, 891, 1028, 1225, 2544
BshTI	ACCGGT	1 cut(s)	3169
BsiHKCI	CYCGRG	1 cut(s)	1133
BsiSI	CCGG	5 cut(s)	939, 1216, 1458, 1857, 3170
BslFI	GGGAC	5 cut(s)	560, 1064, 2568, 2679, 2889
BsmAI	GTCTC	5 cut(s)	174, 616, 1617, 1974, 3045
BsmFI	GGGAC	5 cut(s)	560, 1064, 2568, 2679, 2889
BsmI	GAATGC	5 cut(s)	553, 775, 1714, 2941, 3216
BsnI	GGCC	5 cut(s)	563, 891, 1028, 1225, 2544
Bso31I	GGTCTC	2 cut(s)	1617, 3045
BsoBI	CYCGRG	1 cut(s)	1133
Bsp1286I	GDGCHC	1 cut(s)	255
Bsp13I	TCCGGA	2 cut(s)	938, 1856
Bsp143I	GATC	8 cut(s)	210, 881, 1287, 1398, 1454, 2420, 3078, 3242
BspACI	CCGC	9 cut(s)	1502, 1788, 1801, 2522, 3061, 3199, 3221, 3308, 3340
BspANI	GGCC	5 cut(s)	563, 891, 1028, 1225, 2544
BspEI	TCCGGA	2 cut(s)	938, 1856
BspHI	TCATGA	2 cut(s)	795, 1749
BspLI	GGNNCC	8 cut(s)	1296, 1456, 2592, 2637, 2748, 2814, 2835, 3101
BspMAI	CTGCAG	2 cut(s)	950, 1129
BspPI	GGATC	3 cut(s)	205, 1449, 1462
BspTI	CTTAAG	1 cut(s)	2246
BspTNI	GGTCTC	2 cut(s)	1617, 3045
BsrDI	GCAATG	4 cut(s)	226, 308, 417, 496
BsrFI	RCCGGY	1 cut(s)	3169
BsrI	ACTGG	6 cut(s)	58, 892, 1333, 1456, 3018, 3311
BssAI	RCCGGY	1 cut(s)	3169
BssECI	CCNNGG	9 cut(s)	255, 432, 1699, 2001, 2586, 2696, 2808, 2846, 3227
BssMI	GATC	8 cut(s)	210, 881, 1287, 1398, 1454, 2420, 3078, 3242
BssT1I	CCWWGG	6 cut(s)	255, 432, 2001, 2586, 2808, 2846
Bst2UI	CCWGG	8 cut(s)	140, 243, 930, 1701, 2640, 2698, 2751, 3228
Bst6I	CTCTTC	2 cut(s)	357, 3089
BstAFI	CTTAAG	1 cut(s)	2246
BstAPI	GCANNNNNTGC	1 cut(s)	314
BstBAI	YACGTR	1 cut(s)	1871
BstC8I	GCNNGC	4 cut(s)	315, 378, 593, 2097
BstF5I	GGATG	6 cut(s)	947, 1321, 1420, 1643, 2022, 2856
BstHHI	GCGC	1 cut(s)	3143
BstKTI	GATC	8 cut(s)	213, 884, 1290, 1401, 1457, 2423, 3081, 3245
BstMAI	GTCTC	5 cut(s)	174, 616, 1617, 1974, 3045
BstMBI	GATC	8 cut(s)	210, 881, 1287, 1398, 1454, 2420, 3078, 3242
BstMWI	GCNNNNNNNGC	8 cut(s)	95, 314, 386, 416, 425, 1806, 2003, 2449
BstNI	CCWGG	8 cut(s)	140, 243, 930, 1701, 2640, 2698, 2751, 3228
BstNSI	RCATGY	1 cut(s)	2081
BstSCI	CCNGG	8 cut(s)	138, 241, 928, 1699, 2638, 2696, 2749, 3226
BstSFI	CTRYAG	7 cut(s)	570, 633, 821, 946, 1125, 2991, 3347
BstSLI	GKGCMC	1 cut(s)	255
BstSNI	TACGTA	1 cut(s)	1871
BstV2I	GAAGAC	2 cut(s)	1065, 1321
BstX2I	RGATCY	1 cut(s)	1454
BstXI	CCANNNNNNTGG	3 cut(s)	262, 1316, 1841
BstYI	RGATCY	1 cut(s)	1454
Bsu36I	CCTNAGG	1 cut(s)	2068
BsuI	GTATCC	1 cut(s)	1706
BsuRI	GGCC	5 cut(s)	563, 891, 1028, 1225, 2544
BtsCI	GGATG	6 cut(s)	947, 1321, 1420, 1643, 2022, 2856
BtsI	GCAGTG	3 cut(s)	255, 1647, 2490
BtsIMutI	CAGTG	9 cut(s)	49, 255, 1394, 1647, 1725, 2134, 2346, 2490, 2496
Cac8I	GCNNGC	4 cut(s)	315, 378, 593, 2097
CaiI	CAGNNNCTG	1 cut(s)	1117
CciI	TCATGA	2 cut(s)	795, 1749
CfoI	GCGC	1 cut(s)	3143
Cfr10I	RCCGGY	1 cut(s)	3169
Cfr13I	GGNCC	7 cut(s)	146, 561, 1294, 1352, 1481, 2414, 2542
CsiI	ACCWGGT	2 cut(s)	2638, 2749
Csp6I	GTAC	6 cut(s)	158, 325, 1619, 1863, 2142, 2957
CspAI	ACCGGT	1 cut(s)	3169
CspCI	CAANNNNNGTGG	2 cut(s)	2188, 2223
CviQI	GTAC	6 cut(s)	158, 325, 1619, 1863, 2142, 2957
DpnI	GATC	8 cut(s)	212, 883, 1289, 1400, 1456, 2422, 3080, 3244
DpnII	GATC	8 cut(s)	210, 881, 1287, 1398, 1454, 2420, 3078, 3242
DrdI	GACNNNNNNGTC	1 cut(s)	1586
DseDI	GACNNNNNNGTC	1 cut(s)	1586
Eam1104I	CTCTTC	2 cut(s)	357, 3089
EarI	CTCTTC	2 cut(s)	357, 3089
EciI	GGCGGA	2 cut(s)	3210, 3297
Eco105I	TACGTA	1 cut(s)	1871
Eco130I	CCWWGG	6 cut(s)	255, 432, 2001, 2586, 2808, 2846
Eco147I	AGGCCT	2 cut(s)	1028, 1225
Eco31I	GGTCTC	2 cut(s)	1617, 3045
Eco32I	GATATC	1 cut(s)	1567
Eco47I	GGWCC	5 cut(s)	146, 1294, 1352, 1481, 2414
Eco57I	CTGAAG	3 cut(s)	1216, 1944, 2110
Eco81I	CCTNAGG	1 cut(s)	2068
Eco88I	CYCGRG	1 cut(s)	1133
EcoO109I	RGGNCCY	1 cut(s)	561
EcoRI	GAATTC	2 cut(s)	10, 2014
EcoRII	CCWGG	8 cut(s)	138, 241, 928, 1699, 2638, 2696, 2749, 3226
EcoRV	GATATC	1 cut(s)	1567
EcoT14I	CCWWGG	6 cut(s)	255, 432, 2001, 2586, 2808, 2846
EcoT22I	ATGCAT	2 cut(s)	1433, 1716
ErhI	CCWWGG	6 cut(s)	255, 432, 2001, 2586, 2808, 2846
FaqI	GGGAC	5 cut(s)	560, 1064, 2568, 2679, 2889
FbaI	TGATCA	2 cut(s)	1398, 3078
FblI	GTMKAC	2 cut(s)	441, 1727
FokI	GGATG	6 cut(s)	954, 1328, 1427, 1630, 2009, 2863
FspBI	CTAG	6 cut(s)	527, 911, 1362, 1523, 3251, 3273
FspI	TGCGCA	1 cut(s)	3142
GlaI	GCGC	1 cut(s)	3142
HaeIII	GGCC	5 cut(s)	563, 891, 1028, 1225, 2544
HapII	CCGG	5 cut(s)	939, 1216, 1458, 1857, 3170
HhaI	GCGC	1 cut(s)	3143
Hin6I	GCGC	1 cut(s)	3141
HinP1I	GCGC	1 cut(s)	3141
HincII	GTYRAC	5 cut(s)	442, 712, 1122, 1891, 2507
HindII	GTYRAC	5 cut(s)	442, 712, 1122, 1891, 2507
HindIII	AAGCTT	1 cut(s)	387
HpaII	CCGG	5 cut(s)	939, 1216, 1458, 1857, 3170
HphI	GGTGA	6 cut(s)	653, 1162, 1691, 1772, 2222, 3159
HpyCH4IV	ACGT	4 cut(s)	714, 738, 1870, 2314
HpyF10VI	GCNNNNNNNGC	8 cut(s)	95, 314, 386, 416, 425, 1806, 2003, 2449
HpySE526I	ACGT	4 cut(s)	714, 738, 1870, 2314
HspAI	GCGC	1 cut(s)	3141
Kpn2I	TCCGGA	2 cut(s)	938, 1856
Ksp22I	TGATCA	2 cut(s)	1398, 3078
Kzo9I	GATC	8 cut(s)	210, 881, 1287, 1398, 1454, 2420, 3078, 3242
LmnI	GCTCC	8 cut(s)	245, 811, 931, 1114, 1668, 2596, 2818, 2839
LweI	GCATC	3 cut(s)	360, 932, 3148
MabI	ACCWGGT	2 cut(s)	2638, 2749
MaeI	CTAG	6 cut(s)	527, 911, 1362, 1523, 3251, 3273
MaeII	ACGT	4 cut(s)	714, 738, 1870, 2314
MalI	GATC	8 cut(s)	212, 883, 1289, 1400, 1456, 2422, 3080, 3244
MboI	GATC	8 cut(s)	210, 881, 1287, 1398, 1454, 2420, 3078, 3242
MfeI	CAATTG	1 cut(s)	3066
MflI	RGATCY	1 cut(s)	1454
MhlI	GDGCHC	1 cut(s)	255
MlyI	GAGTC	4 cut(s)	695, 926, 2057, 2251
MmeI	TCCRAC	6 cut(s)	417, 1379, 2408, 2613, 2627, 2724
Mph1103I	ATGCAT	2 cut(s)	1433, 1716
MroI	TCCGGA	2 cut(s)	938, 1856
MroXI	GAANNNNTTC	1 cut(s)	1328
MslI	CAYNNNNRTG	1 cut(s)	1839
MspA1I	CMGCKG	2 cut(s)	1499, 2522
MspCI	CTTAAG	1 cut(s)	2246
MspI	CCGG	5 cut(s)	939, 1216, 1458, 1857, 3170
MspR9I	CCNGG	8 cut(s)	140, 243, 930, 1701, 2640, 2698, 2751, 3228
MunI	CAATTG	1 cut(s)	3066
Mva1269I	GAATGC	5 cut(s)	553, 775, 1714, 2941, 3216
MvaI	CCWGG	8 cut(s)	140, 243, 930, 1701, 2640, 2698, 2751, 3228
MwoI	GCNNNNNNNGC	8 cut(s)	95, 314, 386, 416, 425, 1806, 2003, 2449
NdeII	GATC	8 cut(s)	210, 881, 1287, 1398, 1454, 2420, 3078, 3242
NlaIV	GGNNCC	8 cut(s)	1296, 1456, 2592, 2637, 2748, 2814, 2835, 3101
NmeAIII	GCCGAG	1 cut(s)	132
NmuCI	GTSAC	5 cut(s)	641, 1278, 2485, 2527, 2980
NsbI	TGCGCA	1 cut(s)	3142
NsiI	ATGCAT	2 cut(s)	1433, 1716
NspI	RCATGY	1 cut(s)	2081
PagI	TCATGA	2 cut(s)	795, 1749
PceI	AGGCCT	2 cut(s)	1028, 1225
PctI	GAATGC	5 cut(s)	553, 775, 1714, 2941, 3216
PdmI	GAANNNNTTC	1 cut(s)	1328
PfoI	TCCNGGA	1 cut(s)	928
PinAI	ACCGGT	1 cut(s)	3169
PleI	GAGTC	4 cut(s)	695, 925, 2057, 2250
PpsI	GAGTC	4 cut(s)	695, 925, 2057, 2250
Ppu21I	YACGTR	1 cut(s)	1871
PshBI	ATTAAT	1 cut(s)	1515
Psp1406I	AACGTT	1 cut(s)	738
Psp6I	CCWGG	8 cut(s)	138, 241, 928, 1699, 2638, 2696, 2749, 3226
PspGI	CCWGG	8 cut(s)	138, 241, 928, 1699, 2638, 2696, 2749, 3226
PspN4I	GGNNCC	8 cut(s)	1296, 1456, 2592, 2637, 2748, 2814, 2835, 3101
PspPI	GGNCC	7 cut(s)	146, 561, 1294, 1352, 1481, 2414, 2542
PsrI	GAACNNNNNNTAC	2 cut(s)	1765, 1797
PstI	CTGCAG	2 cut(s)	950, 1129
PstNI	CAGNNNCTG	1 cut(s)	1117
PsuI	RGATCY	1 cut(s)	1454
PvuII	CAGCTG	1 cut(s)	1499
RsaI	GTAC	6 cut(s)	159, 326, 1620, 1864, 2143, 2958
RsaNI	GTAC	6 cut(s)	158, 325, 1619, 1863, 2142, 2957
RseI	CAYNNNNRTG	1 cut(s)	1839
SalI	GTCGAC	1 cut(s)	440
Sau3AI	GATC	8 cut(s)	210, 881, 1287, 1398, 1454, 2420, 3078, 3242
Sau96I	GGNCC	7 cut(s)	146, 561, 1294, 1352, 1481, 2414, 2542
ScaI	AGTACT	1 cut(s)	326
SchI	GAGTC	4 cut(s)	695, 926, 2057, 2251
ScrFI	CCNGG	8 cut(s)	140, 243, 930, 1701, 2640, 2698, 2751, 3228
SduI	GDGCHC	1 cut(s)	255
SexAI	ACCWGGT	2 cut(s)	2638, 2749
SfaNI	GCATC	3 cut(s)	360, 932, 3148
SfcI	CTRYAG	7 cut(s)	570, 633, 821, 946, 1125, 2991, 3347
SinI	GGWCC	5 cut(s)	146, 1294, 1352, 1481, 2414
SmiMI	CAYNNNNRTG	1 cut(s)	1839
SmlI	CTYRAG	5 cut(s)	196, 303, 1022, 1690, 2246
SmoI	CTYRAG	5 cut(s)	196, 303, 1022, 1690, 2246
SnaBI	TACGTA	1 cut(s)	1871
SpeI	ACTAGT	1 cut(s)	3272
SseBI	AGGCCT	2 cut(s)	1028, 1225
SsiI	CCGC	9 cut(s)	1502, 1788, 1801, 2522, 3061, 3199, 3221, 3308, 3340
SspI	AATATT	1 cut(s)	1201
SspMI	CTAG	6 cut(s)	527, 911, 1362, 1523, 3251, 3273
StuI	AGGCCT	2 cut(s)	1028, 1225
StyD4I	CCNGG	8 cut(s)	138, 241, 928, 1699, 2638, 2696, 2749, 3226
StyI	CCWWGG	6 cut(s)	255, 432, 2001, 2586, 2808, 2846
TaiI	ACGT	4 cut(s)	717, 741, 1873, 2317
TaqI	TCGA	4 cut(s)	399, 441, 1204, 1599
TatI	WGTACW	1 cut(s)	324
TauI	GCSGC	3 cut(s)	1803, 2525, 3202
TscAI	CASTG	9 cut(s)	49, 255, 1401, 1654, 1725, 2134, 2353, 2490, 2496
TseFI	GTSAC	5 cut(s)	641, 1278, 2485, 2527, 2980
Tsp45I	GTSAC	5 cut(s)	641, 1278, 2485, 2527, 2980
TspGWI	ACGGA	4 cut(s)	329, 661, 1681, 3305
TspRI	CASTG	9 cut(s)	49, 255, 1401, 1654, 1725, 2134, 2353, 2490, 2496
Vha464I	CTTAAG	1 cut(s)	2246
VpaK11BI	GGWCC	5 cut(s)	146, 1294, 1352, 1481, 2414
VspI	ATTAAT	1 cut(s)	1515
XapI	RAATTY	6 cut(s)	10, 934, 1602, 1754, 2014, 3163
XbaI	TCTAGA	1 cut(s)	910
XceI	RCATGY	1 cut(s)	2081
XmiI	GTMKAC	2 cut(s)	441, 1727
XmnI	GAANNNNTTC	1 cut(s)	1328
XspI	CTAG	6 cut(s)	527, 911, 1362, 1523, 3251, 3273
ZrmI	AGTACT	1 cut(s)	326
Zsp2I	ATGCAT	2 cut(s)	1433, 1716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.