MD14G1123200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0018436
Physical Location & Seq
Forward (+)
19768125 .. 19768948
824 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1123200.v1.1.491

Sequence Viewer

Length: 363 bp
ATGGCCTCAATGCAATGCCACAAGCCTGCCACTAATGAGGCATGCCCCAAAACTTCCCAAAACATGCCTGCCAGTGAGAGCTCATTAGGGCAAAAGGTGTCTGGCTTTCTGAAAGGTCACCATGGCTCTCACAGCAGCAGCCACACTCAGCGCTCAGCCACCGCAACTAACGGTCAGGGGCACCATGCAACTAACGGCACGGCGTGCCATGGAAGAACCAAGAAGAAGGGTGAGCACAGGAACAAGAAGACAGGAGGAGGCAGAAATCTTCTTCAGAAGATCAAGGATGGTATTTCCGGCCATAGCAGCAGCAGCAGCGACGGCGAGAGCGACAAGGAAGGCAGCTGCAGAAAGAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

12.59

Weight (kDa)

9.92

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018436)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g04390
malus_domestica MD14G1123200.v1.1
prunus_persica Prupe.5G117100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0195171
rosa_laevigata RLG00000004140
rosa_roxburghii Rroxscaffold_3G00260700
rosa_samantha Rh7AG148700 Rh7CG153300 Rh7DG149800
rosa_wichuraiana Rw7G012680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 180
AciI CCGC 1 cut(s) 162
AcoI YGGCCR 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 257
AdeI CACNNNGTG 1 cut(s) 204
AfeI AGCGCT 1 cut(s) 152
AluBI AGCT 2 cut(s) 81, 345
AluI AGCT 2 cut(s) 81, 345
Alw21I GWGCWC 2 cut(s) 83, 237
Aor51HI AGCGCT 1 cut(s) 152
AoxI GGCC 2 cut(s) 3, 298
ApeKI GCWGC 8 cut(s) 135, 138, 306, 309, 312, 315, 342, 345
AspLEI GCGC 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 110, 242
BaeGI GKGCMC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 180
BanII GRGCYC 1 cut(s) 83
BbsI GAAGAC 1 cut(s) 254
Bbv12I GWGCWC 2 cut(s) 83, 237
BbvI GCAGC 8 cut(s) 147, 150, 318, 321, 324, 327, 332, 354
BccI CCATC 1 cut(s) 281
BceAI ACGGC 3 cut(s) 211, 216, 337
BfmI CTRYAG 1 cut(s) 346
BfoI RGCGCY 1 cut(s) 154
BisI GCNGC 8 cut(s) 136, 139, 307, 310, 313, 316, 343, 346
BlpI GCTNAGC 1 cut(s) 154
BlsI GCNGC 8 cut(s) 137, 140, 308, 311, 314, 317, 344, 347
BmiI GGNNCC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 254
Bpu1102I GCTNAGC 1 cut(s) 154
BsaJI CCNNGG 2 cut(s) 121, 208
Bse1I ACTGG 1 cut(s) 72
Bse3DI GCAATG 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 121, 208
BseGI GGATG 1 cut(s) 292
BseMI GCAATG 1 cut(s) 20
BseMII CTCAG 2 cut(s) 161, 168
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 270
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 8 cut(s) 147, 150, 318, 321, 324, 327, 332, 354
BshFI GGCC 2 cut(s) 5, 300
BshNI GGYRCC 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 83, 237
BsiSI CCGG 1 cut(s) 297
BsnI GGCC 2 cut(s) 5, 300
Bsp1286I GDGCHC 3 cut(s) 83, 183, 237
Bsp143I GATC 1 cut(s) 279
Bsp1720I GCTNAGC 1 cut(s) 154
Bsp19I CCATGG 2 cut(s) 121, 208
BspACI CCGC 1 cut(s) 162
BspANI GGCC 2 cut(s) 5, 300
BspCNI CTCAG 2 cut(s) 160, 167
BspLI GGNNCC 1 cut(s) 182
BspMAI CTGCAG 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 20
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 2 cut(s) 121, 208
BssMI GATC 1 cut(s) 279
BssT1I CCWWGG 2 cut(s) 121, 208
Bst4CI ACNGT 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 204
BstC8I GCNNGC 4 cut(s) 27, 43, 69, 205
BstDEI CTNAG 2 cut(s) 147, 154
BstDSI CCRYGG 2 cut(s) 121, 208
BstEII GGTNACC 1 cut(s) 116
BstF5I GGATG 1 cut(s) 292
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 1 cut(s) 282
BstMBI GATC 1 cut(s) 279
BstMWI GCNNNNNNNGC 6 cut(s) 132, 204, 306, 312, 315, 321
BstNSI RCATGY 2 cut(s) 45, 67
BstPI GGTNACC 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 346
BstSLI GKGCMC 1 cut(s) 183
BstV1I GCAGC 8 cut(s) 147, 150, 318, 321, 324, 327, 332, 354
BstV2I GAAGAC 1 cut(s) 254
BsuRI GGCC 2 cut(s) 5, 300
BtgI CCRYGG 2 cut(s) 121, 208
BtsCI GGATG 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 79
Cac8I GCNNGC 4 cut(s) 27, 43, 69, 205
CfoI GCGC 1 cut(s) 153
CviAII CATG 5 cut(s) 42, 64, 122, 185, 209
CviJI RGCY 9 cut(s) 5, 25, 81, 105, 126, 141, 158, 300, 345
CviKI_1 RGCY 9 cut(s) 5, 25, 81, 105, 126, 141, 158, 300, 345
DdeI CTNAG 2 cut(s) 147, 154
DpnI GATC 1 cut(s) 281
DpnII GATC 1 cut(s) 279
DraIII CACNNNGTG 1 cut(s) 204
EaeI YGGCCR 1 cut(s) 298
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 121, 208
Eco24I GRGCYC 1 cut(s) 83
Eco47III AGCGCT 1 cut(s) 152
Eco53kI GAGCTC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 81
EcoO65I GGTNACC 1 cut(s) 116
EcoT14I CCWWGG 2 cut(s) 121, 208
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 2 cut(s) 121, 208
FaeI CATG 5 cut(s) 45, 67, 125, 188, 212
FaiI YATR 6 cut(s) 43, 65, 123, 186, 210, 303
FatI CATG 5 cut(s) 41, 63, 121, 184, 208
Fnu4HI GCNGC 8 cut(s) 136, 139, 307, 310, 313, 316, 343, 346
FokI GGATG 1 cut(s) 299
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 8 cut(s) 136, 139, 307, 310, 313, 316, 343, 346
GlaI GCGC 1 cut(s) 152
GluI GCNGC 8 cut(s) 136, 139, 307, 310, 313, 316, 343, 346
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 2 cut(s) 5, 300
HapII CCGG 1 cut(s) 297
HhaI GCGC 1 cut(s) 153
Hin1II CATG 5 cut(s) 45, 67, 125, 188, 212
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HpaII CCGG 1 cut(s) 297
HphI GGTGA 2 cut(s) 110, 242
Hpy188I TCNGA 2 cut(s) 111, 276
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 2 cut(s) 220, 332
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 3 cut(s) 13, 188, 348
HpyF10VI GCNNNNNNNGC 6 cut(s) 132, 204, 306, 312, 315, 321
HpyF3I CTNAG 2 cut(s) 147, 154
Hsp92II CATG 5 cut(s) 45, 67, 125, 188, 212
HspAI GCGC 1 cut(s) 151
Kzo9I GATC 1 cut(s) 279
LpnPI CCDG 8 cut(s) 39, 81, 85, 87, 161, 223, 237, 310
Lsp1109I GCAGC 8 cut(s) 147, 150, 318, 321, 324, 327, 332, 354
MaeIII GTNAC 1 cut(s) 116
MalI GATC 1 cut(s) 281
MboI GATC 1 cut(s) 279
MboII GAAGA 6 cut(s) 225, 235, 259, 260, 263, 289
MhlI GDGCHC 3 cut(s) 83, 183, 237
MnlI CCTC 4 cut(s) 16, 31, 248, 251
MspA1I CMGCKG 1 cut(s) 345
MspI CCGG 1 cut(s) 297
MwoI GCNNNNNNNGC 6 cut(s) 132, 204, 306, 312, 315, 321
NcoI CCATGG 2 cut(s) 121, 208
NdeII GATC 1 cut(s) 279
NlaIII CATG 5 cut(s) 45, 67, 125, 188, 212
NlaIV GGNNCC 1 cut(s) 182
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 2 cut(s) 45, 67
PaeI GCATGC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PkrI GCNGC 8 cut(s) 137, 140, 308, 311, 314, 317, 344, 347
Psp124BI GAGCTC 1 cut(s) 83
PspEI GGTNACC 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 182
PstI CTGCAG 1 cut(s) 350
PvuII CAGCTG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 83
SatI GCNGC 8 cut(s) 136, 139, 307, 310, 313, 316, 343, 346
Sau3AI GATC 1 cut(s) 279
SduI GDGCHC 3 cut(s) 83, 183, 237
SetI ASST 4 cut(s) 83, 99, 118, 347
SfcI CTRYAG 1 cut(s) 346
SphI GCATGC 1 cut(s) 45
SsiI CCGC 1 cut(s) 162
SstI GAGCTC 1 cut(s) 83
StyI CCWWGG 2 cut(s) 121, 208
TaaI ACNGT 1 cut(s) 173
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 8 cut(s) 135, 138, 306, 309, 312, 315, 342, 345
Tsp45I GTSAC 1 cut(s) 116
TspRI CASTG 1 cut(s) 79
XceI RCATGY 2 cut(s) 45, 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.