RLG00000004140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018436
Physical Location & Seq
Reverse (-)
56537197 .. 56537843
647 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004140

Sequence Viewer

Length: 435 bp
ATGGCCTCAATCCAATGCTACAAGCCTGCCAATGAGTCATGCCAGCAAAAATGCCACAAGGCTAGCAGCAACCAGAGCTCATCTCTGGGCCAAAAGGTGTCTGAGATGGCTAGCTTCTTGAAAGGAGGAGGTCATCGTATGCATGGACAACCACACACCACCACCACCACGGAATGCTACAGCCAAACCGTTACCTACAGGACCTCTGAAAACCAGCTGGGCTTGGGCGGCCCCAAGCACCACGTAGGTAGTACTACTGATGCAGTGGTGGTATGCAATGGAAAGACCAAGAAGACTAGGGGTAATCGCCACACGAAGGAGAGAGACAGCAGAAATCTGTTTCAGAAGATCAAGGATGGGAGTTCTGGTGATAGCAGCTGCAGTGACAGTGAGAGCGACAGTGACCACGAAGGCAACCGCAAACACAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

15.69

Weight (kDa)

9.2

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018436)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g04390
malus_domestica MD14G1123200.v1.1
prunus_persica Prupe.5G117100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0195171
rosa_laevigata RLG00000004140
rosa_roxburghii Rroxscaffold_3G00260700
rosa_samantha Rh7AG148700 Rh7CG153300 Rh7DG149800
rosa_wichuraiana Rw7G012680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 228, 418
AfaI GTAC 1 cut(s) 253
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 1 cut(s) 121
AluBI AGCT 4 cut(s) 78, 114, 217, 378
AluI AGCT 4 cut(s) 78, 114, 217, 378
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 1 cut(s) 318
AoxI GGCC 3 cut(s) 3, 88, 229
ApeKI GCWGC 3 cut(s) 66, 375, 378
AspS9I GGNCC 3 cut(s) 88, 201, 230
AsuHPI GGTGA 1 cut(s) 380
AsuNHI GCTAGC 2 cut(s) 62, 110
AvaII GGWCC 1 cut(s) 201
BanII GRGCYC 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 3 cut(s) 78, 365, 387
BccI CCATC 2 cut(s) 100, 350
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 3 cut(s) 63, 111, 297
BfmI CTRYAG 3 cut(s) 178, 196, 379
BisI GCNGC 4 cut(s) 67, 229, 376, 379
BlsI GCNGC 4 cut(s) 68, 230, 377, 380
BmcAI AGTACT 1 cut(s) 253
Bme18I GGWCC 1 cut(s) 201
BmgT120I GGNCC 3 cut(s) 88, 201, 230
BmiI GGNNCC 1 cut(s) 232
BmsI GCATC 1 cut(s) 250
BmtI GCTAGC 2 cut(s) 66, 114
BpiI GAAGAC 1 cut(s) 299
BplI GAGNNNNNCTC 2 cut(s) 67, 99
BsaAI YACGTR 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 168
BsaXI ACNNNNNCTCC 2 cut(s) 352, 382
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 141
BseXI GCAGC 3 cut(s) 78, 365, 387
BseYI CCCAGC 1 cut(s) 217
BshFI GGCC 3 cut(s) 5, 90, 231
BsiHKAI GWGCWC 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 318
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 3 cut(s) 5, 90, 231
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 1 cut(s) 348
BspACI CCGC 2 cut(s) 228, 418
BspANI GGCC 3 cut(s) 5, 90, 231
BspCNI CTCAG 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 232
BspMAI CTGCAG 1 cut(s) 383
BspOI GCTAGC 2 cut(s) 66, 114
BsrDI GCAATG 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 1 cut(s) 348
Bst4CI ACNGT 3 cut(s) 190, 389, 401
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 4 cut(s) 27, 44, 64, 112
BstDEI CTNAG 1 cut(s) 102
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 1 cut(s) 351
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 1 cut(s) 348
BstMWI GCNNNNNNNGC 2 cut(s) 75, 228
BstSFI CTRYAG 3 cut(s) 178, 196, 379
BstV1I GCAGC 3 cut(s) 78, 365, 387
BstV2I GAAGAC 1 cut(s) 299
BsuRI GGCC 3 cut(s) 5, 90, 231
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 1 cut(s) 361
BtsI GCAGTG 2 cut(s) 270, 388
BtsIMutI CAGTG 4 cut(s) 270, 388, 394, 406
Cac8I GCNNGC 4 cut(s) 27, 44, 64, 112
Cfr13I GGNCC 3 cut(s) 88, 201, 230
Csp6I GTAC 1 cut(s) 252
CviAII CATG 2 cut(s) 39, 143
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 1 cut(s) 102
DpnI GATC 1 cut(s) 350
DpnII GATC 1 cut(s) 348
Ecl136II GAGCTC 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 78
EcoO109I RGGNCCY 1 cut(s) 201
EcoT22I ATGCAT 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 80
FaeI CATG 2 cut(s) 42, 146
FaiI YATR 4 cut(s) 40, 140, 144, 274
FatI CATG 2 cut(s) 38, 142
Fnu4HI GCNGC 4 cut(s) 67, 229, 376, 379
FokI GGATG 1 cut(s) 368
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 4 cut(s) 67, 229, 376, 379
FspBI CTAG 3 cut(s) 63, 111, 297
GluI GCNGC 4 cut(s) 67, 229, 376, 379
GsaI CCCAGC 1 cut(s) 221
HaeIII GGCC 3 cut(s) 5, 90, 231
Hin1II CATG 2 cut(s) 42, 146
HinfI GANTC 1 cut(s) 35
HphI GGTGA 1 cut(s) 380
Hpy188I TCNGA 3 cut(s) 103, 208, 345
Hpy188III TCNNGA 1 cut(s) 118
HpyAV CCTTC 2 cut(s) 310, 404
HpyCH4III ACNGT 3 cut(s) 190, 389, 401
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 4 cut(s) 142, 263, 276, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 228
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 2 cut(s) 42, 146
Kzo9I GATC 1 cut(s) 348
LpnPI CCDG 8 cut(s) 39, 56, 71, 86, 184, 203, 227, 351
Lsp1109I GCAGC 3 cut(s) 78, 365, 387
LweI GCATC 1 cut(s) 250
MaeI CTAG 3 cut(s) 63, 111, 297
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 3 cut(s) 190, 383, 401
MalI GATC 1 cut(s) 350
MboI GATC 1 cut(s) 348
MboII GAAGA 2 cut(s) 304, 358
MhlI GDGCHC 1 cut(s) 80
MlyI GAGTC 1 cut(s) 44
MnlI CCTC 4 cut(s) 16, 119, 122, 214
Mph1103I ATGCAT 1 cut(s) 144
MspA1I CMGCKG 2 cut(s) 217, 378
Mva1269I GAATGC 1 cut(s) 179
MwoI GCNNNNNNNGC 2 cut(s) 75, 228
NdeII GATC 1 cut(s) 348
NheI GCTAGC 2 cut(s) 62, 110
NlaIII CATG 2 cut(s) 42, 146
NlaIV GGNNCC 1 cut(s) 232
NmuCI GTSAC 2 cut(s) 383, 401
NsiI ATGCAT 1 cut(s) 144
PctI GAATGC 1 cut(s) 179
PkrI GCNGC 4 cut(s) 68, 230, 377, 380
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Ppu21I YACGTR 1 cut(s) 244
PpuMI RGGWCCY 1 cut(s) 201
Psp124BI GAGCTC 1 cut(s) 80
Psp5II RGGWCCY 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 3 cut(s) 88, 201, 230
PspPPI RGGWCCY 1 cut(s) 201
PstI CTGCAG 1 cut(s) 383
PvuII CAGCTG 2 cut(s) 217, 378
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
SacI GAGCTC 1 cut(s) 80
SatI GCNGC 4 cut(s) 67, 229, 376, 379
Sau3AI GATC 1 cut(s) 348
Sau96I GGNCC 3 cut(s) 88, 201, 230
ScaI AGTACT 1 cut(s) 253
SchI GAGTC 1 cut(s) 44
SduI GDGCHC 1 cut(s) 80
SfaNI GCATC 1 cut(s) 250
SfcI CTRYAG 3 cut(s) 178, 196, 379
SinI GGWCC 1 cut(s) 201
SsiI CCGC 2 cut(s) 228, 418
SspMI CTAG 3 cut(s) 63, 111, 297
SstI GAGCTC 1 cut(s) 80
TaaI ACNGT 3 cut(s) 190, 389, 401
TaiI ACGT 1 cut(s) 246
TatI WGTACW 1 cut(s) 251
TauI GCSGC 1 cut(s) 231
TscAI CASTG 4 cut(s) 270, 388, 394, 406
TseFI GTSAC 2 cut(s) 383, 401
TseI GCWGC 3 cut(s) 66, 375, 378
Tsp45I GTSAC 2 cut(s) 383, 401
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 4 cut(s) 270, 388, 394, 406
VpaK11BI GGWCC 1 cut(s) 201
XspI CTAG 3 cut(s) 63, 111, 297
ZrmI AGTACT 1 cut(s) 253
Zsp2I ATGCAT 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.