Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012968

Physical Location & Seq

Genomic Coordinates Forward (+)

21282994 .. 21290297

Gene Structure 7304 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1134300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGACGGTGGCTAGCTCAGTGGTCCACGAAGTGCTGGGCCGGCGGGCCGAGGACGTCGATGAGCCCATAATCGAGTACATCGTTAACGTCCTCGCCGACGAGGACTTCGATTTCGGCGACGACGGTGAAGGCGCCTTCGACGCTCTCGGCGAGCTCCTCGTCGGAGCCGGCTGTGTTAGCGACTTCGCTGAGTGCCGCTCGGTTTGCAGTATAATATCCGAGAAGTTCGGGAAACATGGGTTGGTTAAAGCTAAACCGACTGTGCGGAGCCTATCAGCACCAGTAAGAATGGATGATGGGATGGATGAGAAGGTAGCTCCAAAGAAGAAGGTTGAGGTGATTGATGGCCCTCTGTTAACTGAACGTGACCGAGCTAAGATAGAGAGGAAAAAGAGGAAGGATGATCGCCAAAGAGAGCAACAATATCAAATACATTTAGCAGAGATGGAAGCAGTCAGGGCAGGAATGCCTGTTGTATCCGTAAATCATGAAAGTGCTGGTGGCCCAAATGTCAGGGATATCCGTCTGGAAAACTTCAATGTTTCTGTGGGTGGCCGTGATCTAATTGTGGATGGTTCAGTAACACTTTCTTTTGGAAGGCATTATGGCCTTGTTGGAAGAAATGGTACGGGGAAAACAACGTTTCTTAGGCACTTGGCTATGCATGCCATTGACGGCATTCCTAGGAACTTCCAAATATTGCATGTGGAGCAAGAAGTGGTGGGTGATGATACAACAGCCTTGCAGTGTGTTCTTAACACTGATGTTGAAAGAACAAAGCTTTTGGAAGAAGAAGCTCGTCTTCTCACCCAACAGAGAGCATTGGAGTTTGAGGACTCAACAGAAAAGAGTAACGGGGAAGTTGATAAAGATGCCATTGGGCAGAGGCTTCAGGAGATATACAAAAGGCTCGAGTTCATTGATGCTGATTCGGCAGAGTCACGGGCAGCTTCCATTCTTGCGGGTCTTAGTTTCTCTCCGGAGATGCAGTTGAAACCAACTAAAGCATTTTCAGGAGGATGGCGGATGCGAATAGCTCTTGCTCGTGCCCTGTTTATAGAGCCTGATTTGTTACTGCTTGATGAACCCACGAACCATCTTGATCTTCATGCAGTCCTCTGGCTAGAGGCTTACCTGGTGAAGTGGCCAAAAACATGCATAGTTGTTTCTCATGCTAGAGAATTCTTGAACACGGTAGTCACTGATATACTACATCTTCATGGGCAAAAATTGAATGCTTACAAGGGAGACTACGATACATATGAGAGGACGCGGATAGAACTAGTCAAGAACCAACAGAAGGCTTTTGAGGCCAATGAGCGTTCAAGAACACATATGCAGTCGTTTATTGATAAGTTCCGCTACAATGCCAAGAGAGCTGCGCTTGTTCAGTCAAGAATTAAGGCTTTGGACCGGTTGGGACATGTGGATGAGATTGTAAATGACCCTGACTATAAGTTTGAGTTCCCAACTCCAGACGACAGACCTGGTCCTCCAATAATAAGTTTCAGTGATGCATCATTTGGTTATCCTGGGGGACCCGTCTTGTTTAGGAATTTAAATTTTGGAATTGACCTTGACAGCCGCATCGCAATGGTCGGGCCAAATGGTATTGGTAAATCAACGATACTCAAACTAATTGCAGGGGAGCTACAACCAATCTCTGGGACAGTTTTTCGTTCAGCTAAGGTTCGGATCGCTGTATTCAGTCAGCACCATGTTGACGGACTAGATTTATCTTCTAATCCCCTATTGTATATGATGCGCTGCTTCCCGGGGGTGCCTGAACAAAAGCTCCGATCTCACTTAGGTTCTTTCGGTGTCAGTGGAAATCTTGCACTTCAGCCTATGTACACATTGTCTGGTGGTCAGAAGAGCAGAGTTGCATTTGCCAAGATAACTTTCAAGAAACCGCACATAATACTGCTTGATGAGCCCTCCAATCATCTGGACTTGGATGCTGTGGAGGCACTAATTCAAGGTCTTGTTCTCTTCCAAGGAGGCATTCTCATGGTTAGTCACGACGAGCATCTGATCTCTGGAAGTGTGGACGAGCTGTGGGTGGTATCAGAAGGTAGGATACAACCATTCCATGGCAGTTTTGAGGACTACAAGAAGATACTGCAGTCCTCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0010035 GO:0010038 GO:0042221 GO:0042742 GO:0043207 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC_tran ABC_tran_Xtn

Protein Analysis

712

Amino Acids

79.17

Weight (kDa)

5.79

Isoelectric Point (pI)

35.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PWI_ABCF3	PF26051	6 - 78	5e-15	ABCF3, PWI-like helical bundle domain
ABC_tran	PF00005	193 - 365	1.5e-17	ABC transporter
ABC_tran_Xtn	PF12848	404 - 484	9.3e-23	ABC transporter
ABC_tran	PF00005	518 - 648	6e-22	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64550
fragaria_vesca	FvH4_5g11480 FvH4_5g11480
malus_domestica	MD06G1113200.v1.1 MD14G1134300.v1.1
prunus_persica	Prupe.5G126100_v2.0.a1 Prupe.5G126100_v2.0.a1
pyrus_communis	pycom06g10590
rosa_chinensis	RchiOBHm_Chr7g0186321
rosa_laevigata	RLG00000004849 RLG00000034878
rosa_multiflora	Rmu_sc0000645.1_g000050
rosa_roxburghii	Rroxscaffold_3G00268220
rosa_rugosa	Rorug06G0473500
rosa_samantha	Rh7AG067700 Rh7BG083500 Rh7CG080300
rosa_wichuraiana	Rw7G006730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	57
AccB1I	GGYRCC	2 cut(s)	131, 1780
AccB7I	CCANNNNNTGG	2 cut(s)	1664, 2093
AccBSI	CCGCTC	1 cut(s)	198
AccII	CGCG	1 cut(s)	1273
AccIII	TCCGGA	1 cut(s)	979
AciI	CCGC	9 cut(s)	43, 196, 265, 962, 1024, 1273, 1360, 1585, 1913
AclI	AACGTT	1 cut(s)	641
AclWI	GGATC	1 cut(s)	1703
AcoI	YGGCCR	2 cut(s)	553, 1145
AcsI	RAATTY	3 cut(s)	1181, 1555, 1561
AcuI	CTGAAG	2 cut(s)	875, 1826
AcyI	GRCGYC	2 cut(s)	54, 132
AdeI	CACNNNGTG	1 cut(s)	31
AfaI	GTAC	3 cut(s)	77, 628, 1853
AfiI	CCNNNNNNNGG	3 cut(s)	1300, 1664, 2093
AflIII	ACRYGT	1 cut(s)	1423
AgeI	ACCGGT	1 cut(s)	1413
AgsI	TTSAA	8 cut(s)	538, 770, 994, 1189, 1234, 1326, 1906, 1979
AhlI	ACTAGT	1 cut(s)	1282
AjnI	CCWGG	3 cut(s)	1134, 1486, 1531
AjuI	GAANNNNNNNTTGG	6 cut(s)	224, 256, 1886, 1918, 1989, 2021
AloI	GAACNNNNNNTCC	2 cut(s)	1779, 1811
Alw21I	GWGCWC	1 cut(s)	156
Alw26I	GTCTC	1 cut(s)	1242
AlwI	GGATC	1 cut(s)	1703
Ama87I	CYCGRG	2 cut(s)	911, 1774
Aor13HI	TCCGGA	1 cut(s)	979
AoxI	GGCC	9 cut(s)	37, 45, 346, 502, 553, 607, 1145, 1311, 1601
ApeKI	GCWGC	3 cut(s)	947, 1379, 1767
ApoI	RAATTY	3 cut(s)	1181, 1555, 1561
ArsI	GACNNNNNNTTYG	6 cut(s)	89, 95, 121, 127, 1442, 1474
AsiGI	ACCGGT	1 cut(s)	1413
AspA2I	CCTAGG	1 cut(s)	683
AspLEI	GCGC	3 cut(s)	134, 1384, 1767
AspS9I	GGNCC	9 cut(s)	22, 37, 45, 347, 503, 1411, 1490, 1538, 1601
AsuC2I	CCSGG	2 cut(s)	1775, 1776
AsuHPI	GGTGA	5 cut(s)	137, 349, 737, 799, 1150
AsuNHI	GCTAGC	1 cut(s)	11
AvaI	CYCGRG	2 cut(s)	911, 1774
AvaII	GGWCC	4 cut(s)	22, 1411, 1490, 1538
AvrII	CCTAGG	1 cut(s)	683
BaeGI	GKGCMC	1 cut(s)	1051
BaeI	ACNNNNGTAYC	1 cut(s)	2111
BalI	TGGCCA	1 cut(s)	1147
BanI	GGYRCC	2 cut(s)	131, 1780
BanII	GRGCYC	3 cut(s)	66, 156, 1938
BarI	GAAGNNNNNNTAC	4 cut(s)	610, 642, 2064, 2096
BauI	CACGAG	1 cut(s)	1044
BbsI	GAAGAC	1 cut(s)	794
Bbv12I	GWGCWC	1 cut(s)	156
BbvI	GCAGC	3 cut(s)	959, 1366, 1754
BccI	CCATC	7 cut(s)	290, 295, 338, 439, 566, 1014, 1104
BceAI	ACGGC	2 cut(s)	540, 691
BciT130I	CCWGG	3 cut(s)	1136, 1488, 1533
BciVI	GTATCC	2 cut(s)	487, 2073
BcnI	CCSGG	2 cut(s)	1775, 1776
BcoDI	GTCTC	1 cut(s)	1242
BcuI	ACTAGT	1 cut(s)	1282
BfaI	CTAG	6 cut(s)	12, 684, 1124, 1176, 1283, 1730
BfmI	CTRYAG	1 cut(s)	2123
BfoI	RGCGCY	1 cut(s)	135
BfuI	GTATCC	2 cut(s)	487, 2073
BisI	GCNGC	5 cut(s)	196, 948, 1380, 1585, 1768
BlnI	CCTAGG	1 cut(s)	683
BlsI	GCNGC	5 cut(s)	197, 949, 1381, 1586, 1769
Bme1390I	CCNGG	5 cut(s)	1136, 1488, 1533, 1775, 1776
Bme18I	GGWCC	4 cut(s)	22, 1411, 1490, 1538
BmeT110I	CYCGRG	2 cut(s)	911, 1774
BmgT120I	GGNCC	9 cut(s)	22, 37, 45, 347, 503, 1411, 1490, 1538, 1601
BmiI	GGNNCC	6 cut(s)	133, 166, 269, 1539, 1540, 1782
BmrFI	CCNGG	5 cut(s)	1136, 1488, 1533, 1775, 1776
BmtI	GCTAGC	1 cut(s)	15
BpiI	GAAGAC	1 cut(s)	794
BplI	GAGNNNNNCTC	4 cut(s)	182, 214, 1992, 2024
BpmI	CTGGAG	1 cut(s)	1458
Bpu10I	CCTNAGC	1 cut(s)	1686
BpuMI	CCSGG	2 cut(s)	1775, 1776
BsaHI	GRCGYC	2 cut(s)	54, 132
BsaJI	CCNNGG	7 cut(s)	48, 683, 1532, 1774, 1775, 1996, 2092
BsaWI	WCCGGW	2 cut(s)	979, 1413
BsaXI	ACNNNNNCTCC	4 cut(s)	974, 1004, 1779, 1809
Bsc4I	CCNNNNNNNGG	3 cut(s)	1300, 1664, 2093
Bse118I	RCCGGY	3 cut(s)	39, 167, 1413
Bse1I	ACTGG	1 cut(s)	281
Bse3DI	GCAATG	1 cut(s)	1599
BseAI	TCCGGA	1 cut(s)	979
BseBI	CCWGG	3 cut(s)	1136, 1488, 1533
BseDI	CCNNGG	7 cut(s)	48, 683, 1532, 1774, 1775, 1996, 2092
BseGI	GGATG	9 cut(s)	298, 306, 310, 406, 577, 1025, 1032, 1435, 1963
BseLI	CCNNNNNNNGG	3 cut(s)	1300, 1664, 2093
BseMI	GCAATG	1 cut(s)	1599
BseMII	CTCAG	2 cut(s)	30, 180
BseNI	ACTGG	1 cut(s)	281
BseRI	GAGGAG	1 cut(s)	146
BseSI	GKGCMC	1 cut(s)	1051
BseXI	GCAGC	3 cut(s)	959, 1366, 1754
BseYI	CCCAGC	1 cut(s)	34
Bsh1236I	CGCG	1 cut(s)	1273
BshFI	GGCC	9 cut(s)	39, 47, 348, 504, 555, 609, 1147, 1313, 1603
BshNI	GGYRCC	2 cut(s)	131, 1780
BshTI	ACCGGT	1 cut(s)	1413
BsiHKAI	GWGCWC	1 cut(s)	156
BsiHKCI	CYCGRG	2 cut(s)	911, 1774
BsiSI	CCGG	5 cut(s)	40, 168, 980, 1414, 1775
BslFI	GGGAC	3 cut(s)	1434, 1551, 1681
BslI	CCNNNNNNNGG	3 cut(s)	1300, 1664, 2093
BsmAI	GTCTC	1 cut(s)	1242
BsmFI	GGGAC	3 cut(s)	1434, 1551, 1681
BsmI	GAATGC	4 cut(s)	471, 678, 1240, 2004
BsnI	GGCC	9 cut(s)	39, 47, 348, 504, 555, 609, 1147, 1313, 1603
BsoBI	CYCGRG	2 cut(s)	911, 1774
Bsp1286I	GDGCHC	4 cut(s)	66, 156, 1051, 1938
Bsp13I	TCCGGA	1 cut(s)	979
Bsp1407I	TGTACA	1 cut(s)	1851
Bsp143I	GATC	6 cut(s)	403, 559, 1102, 1695, 1799, 2034
Bsp19I	CCATGG	1 cut(s)	2092
BspACI	CCGC	9 cut(s)	43, 196, 265, 962, 1024, 1273, 1360, 1585, 1913
BspANI	GGCC	9 cut(s)	39, 47, 348, 504, 555, 609, 1147, 1313, 1603
BspCNI	CTCAG	2 cut(s)	29, 181
BspEI	TCCGGA	1 cut(s)	979
BspFNI	CGCG	1 cut(s)	1273
BspHI	TCATGA	1 cut(s)	487
BspLI	GGNNCC	6 cut(s)	133, 166, 269, 1539, 1540, 1782
BspMAI	CTGCAG	1 cut(s)	2127
BspOI	GCTAGC	1 cut(s)	15
BspPI	GGATC	1 cut(s)	1703
BspQI	GCTCTTC	1 cut(s)	1868
BspT107I	GGYRCC	2 cut(s)	131, 1780
BsrBI	CCGCTC	1 cut(s)	198
BsrDI	GCAATG	1 cut(s)	1599
BsrFI	RCCGGY	3 cut(s)	39, 167, 1413
BsrGI	TGTACA	1 cut(s)	1851
BsrI	ACTGG	1 cut(s)	281
BssAI	RCCGGY	3 cut(s)	39, 167, 1413
BssECI	CCNNGG	7 cut(s)	48, 683, 1532, 1774, 1775, 1996, 2092
BssMI	GATC	6 cut(s)	403, 559, 1102, 1695, 1799, 2034
BssNI	GRCGYC	2 cut(s)	54, 132
BssSI	CACGAG	1 cut(s)	1044
BssT1I	CCWWGG	3 cut(s)	683, 1996, 2092
Bst2BI	CACGAG	1 cut(s)	1044
Bst2UI	CCWGG	3 cut(s)	1136, 1488, 1533
Bst4CI	ACNGT	5 cut(s)	7, 125, 262, 1195, 1672
Bst6I	CTCTTC	2 cut(s)	1868, 1997
BstACI	GRCGYC	2 cut(s)	54, 132
BstAUI	TGTACA	1 cut(s)	1851
BstC8I	GCNNGC	6 cut(s)	13, 41, 45, 152, 169, 666
BstDEI	CTNAG	7 cut(s)	16, 189, 375, 647, 968, 1686, 1807
BstDSI	CCRYGG	1 cut(s)	2092
BstF5I	GGATG	9 cut(s)	298, 306, 310, 406, 577, 1025, 1032, 1435, 1963
BstFNI	CGCG	1 cut(s)	1273
BstH2I	RGCGCY	1 cut(s)	135
BstHHI	GCGC	3 cut(s)	134, 1384, 1767
BstKTI	GATC	6 cut(s)	406, 562, 1105, 1698, 1802, 2037
BstMAI	GTCTC	1 cut(s)	1242
BstMBI	GATC	6 cut(s)	403, 559, 1102, 1695, 1799, 2034
BstNI	CCWGG	3 cut(s)	1136, 1488, 1533
BstNSI	RCATGY	4 cut(s)	668, 707, 1158, 1427
BstSCI	CCNGG	5 cut(s)	1134, 1486, 1531, 1773, 1774
BstSFI	CTRYAG	1 cut(s)	2123
BstSLI	GKGCMC	1 cut(s)	1051
BstUI	CGCG	1 cut(s)	1273
BstV1I	GCAGC	3 cut(s)	959, 1366, 1754
BstV2I	GAAGAC	1 cut(s)	794
BstXI	CCANNNNNNTGG	1 cut(s)	1948
BsuI	GTATCC	2 cut(s)	487, 2073
BsuRI	GGCC	9 cut(s)	39, 47, 348, 504, 555, 609, 1147, 1313, 1603
BtgI	CCRYGG	1 cut(s)	2092
BtgZI	GCGATG	1 cut(s)	1573
BtsCI	GGATG	9 cut(s)	298, 306, 310, 406, 577, 1025, 1032, 1435, 1963
BtsI	GCAGTG	1 cut(s)	752
BtsIMutI	CAGTG	6 cut(s)	24, 752, 759, 1200, 1516, 1831
Cac8I	GCNNGC	6 cut(s)	13, 41, 45, 152, 169, 666
CciI	TCATGA	1 cut(s)	487
CfoI	GCGC	3 cut(s)	134, 1384, 1767
Cfr10I	RCCGGY	3 cut(s)	39, 167, 1413
Cfr13I	GGNCC	9 cut(s)	22, 37, 45, 347, 503, 1411, 1490, 1538, 1601
Cfr9I	CCCGGG	1 cut(s)	1774
CseI	GACGC	2 cut(s)	149, 1279
CsiI	ACCWGGT	2 cut(s)	1134, 1486
Csp6I	GTAC	3 cut(s)	76, 627, 1852
CspAI	ACCGGT	1 cut(s)	1413
CviQI	GTAC	3 cut(s)	76, 627, 1852
DdeI	CTNAG	7 cut(s)	16, 189, 375, 647, 968, 1686, 1807
DinI	GGCGCC	1 cut(s)	133
DpnI	GATC	6 cut(s)	405, 561, 1104, 1697, 1801, 2036
DpnII	GATC	6 cut(s)	403, 559, 1102, 1695, 1799, 2034
DraI	TTTAAA	1 cut(s)	1560
DraIII	CACNNNGTG	1 cut(s)	31
EaeI	YGGCCR	2 cut(s)	553, 1145
Eam1104I	CTCTTC	2 cut(s)	1868, 1997
EarI	CTCTTC	2 cut(s)	1868, 1997
EciI	GGCGGA	1 cut(s)	1039
Ecl136II	GAGCTC	1 cut(s)	154
Eco130I	CCWWGG	3 cut(s)	683, 1996, 2092
Eco24I	GRGCYC	3 cut(s)	66, 156, 1938
Eco32I	GATATC	1 cut(s)	520
Eco47I	GGWCC	4 cut(s)	22, 1411, 1490, 1538
Eco53kI	GAGCTC	1 cut(s)	154
Eco57I	CTGAAG	2 cut(s)	875, 1826
Eco88I	CYCGRG	2 cut(s)	911, 1774
EcoICRI	GAGCTC	1 cut(s)	154
EcoO109I	RGGNCCY	1 cut(s)	1538
EcoRI	GAATTC	1 cut(s)	1181
EcoRII	CCWGG	3 cut(s)	1134, 1486, 1531
EcoRV	GATATC	1 cut(s)	520
EcoT14I	CCWWGG	3 cut(s)	683, 1996, 2092
EcoT22I	ATGCAT	3 cut(s)	666, 1160, 1519
EcoT38I	GRGCYC	3 cut(s)	66, 156, 1938
EgeI	GGCGCC	1 cut(s)	133
EheI	GGCGCC	1 cut(s)	133
ErhI	CCWWGG	3 cut(s)	683, 1996, 2092
FalI	AAGNNNNNCTT	2 cut(s)	786, 818
FaqI	GGGAC	3 cut(s)	1434, 1551, 1681
FauI	CCCGC	2 cut(s)	36, 955
FauNDI	CATATG	2 cut(s)	1261, 1335
Fnu4HI	GCNGC	5 cut(s)	196, 948, 1380, 1585, 1768
FokI	GGATG	9 cut(s)	305, 313, 317, 413, 584, 1032, 1039, 1442, 1970
FriOI	GRGCYC	3 cut(s)	66, 156, 1938
Fsp4HI	GCNGC	5 cut(s)	196, 948, 1380, 1585, 1768
FspBI	CTAG	6 cut(s)	12, 684, 1124, 1176, 1283, 1730
GlaI	GCGC	3 cut(s)	133, 1383, 1766
GluI	GCNGC	5 cut(s)	196, 948, 1380, 1585, 1768
GsaI	CCCAGC	1 cut(s)	38
GsuI	CTGGAG	1 cut(s)	1458
HaeII	RGCGCY	1 cut(s)	135
HaeIII	GGCC	9 cut(s)	39, 47, 348, 504, 555, 609, 1147, 1313, 1603
HapII	CCGG	5 cut(s)	40, 168, 980, 1414, 1775
HgaI	GACGC	2 cut(s)	149, 1279
HhaI	GCGC	3 cut(s)	134, 1384, 1767
Hin1I	GRCGYC	2 cut(s)	54, 132
Hin6I	GCGC	3 cut(s)	132, 1382, 1765
HinP1I	GCGC	3 cut(s)	132, 1382, 1765
HincII	GTYRAC	3 cut(s)	85, 357, 1723
HindII	GTYRAC	3 cut(s)	85, 357, 1723
HindIII	AAGCTT	1 cut(s)	779
HinfI	GANTC	3 cut(s)	836, 929, 938
HpaI	GTTAAC	2 cut(s)	85, 357
HpaII	CCGG	5 cut(s)	40, 168, 980, 1414, 1775
HphI	GGTGA	5 cut(s)	137, 349, 737, 799, 1150
Hpy166II	GTNNAC	6 cut(s)	25, 85, 357, 1723, 1854, 2050
Hpy188I	TCNGA	7 cut(s)	164, 220, 1695, 1799, 1872, 2034, 2071
Hpy8I	GTNNAC	6 cut(s)	25, 85, 357, 1723, 1854, 2050
Hpy99I	CGWCG	7 cut(s)	59, 101, 122, 125, 143, 164, 2027
HpyAV	CCTTC	8 cut(s)	122, 145, 304, 322, 391, 591, 1294, 2066
HpyCH4III	ACNGT	5 cut(s)	7, 125, 262, 1195, 1672
HpyCH4IV	ACGT	4 cut(s)	54, 87, 364, 641
HpyF3I	CTNAG	7 cut(s)	16, 189, 375, 647, 968, 1686, 1807
HpySE526I	ACGT	4 cut(s)	54, 87, 364, 641
Hsp92I	GRCGYC	2 cut(s)	54, 132
HspAI	GCGC	3 cut(s)	132, 1382, 1765
KasI	GGCGCC	1 cut(s)	131
KflI	GGGWCCC	1 cut(s)	1538
Kpn2I	TCCGGA	1 cut(s)	979
KroI	GCCGGC	2 cut(s)	39, 167
KroNI	GCCGGC	2 cut(s)	41, 169
KspAI	GTTAAC	2 cut(s)	85, 357
Kzo9I	GATC	6 cut(s)	403, 559, 1102, 1695, 1799, 2034
LguI	GCTCTTC	1 cut(s)	1868
LmnI	GCTCC	7 cut(s)	159, 164, 267, 322, 709, 1648, 1800
Lsp1109I	GCAGC	3 cut(s)	959, 1366, 1754
MabI	ACCWGGT	2 cut(s)	1134, 1486
MaeI	CTAG	6 cut(s)	12, 684, 1124, 1176, 1283, 1730
MaeII	ACGT	4 cut(s)	54, 87, 364, 641
MaeIII	GTNAC	7 cut(s)	365, 580, 851, 939, 1071, 1198, 2018
MalI	GATC	6 cut(s)	405, 561, 1104, 1697, 1801, 2036
MbiI	CCGCTC	1 cut(s)	198
MboI	GATC	6 cut(s)	403, 559, 1102, 1695, 1799, 2034
MhlI	GDGCHC	4 cut(s)	66, 156, 1051, 1938
MlsI	TGGCCA	1 cut(s)	1147
MluCI	AATT	9 cut(s)	564, 1181, 1229, 1398, 1555, 1561, 1569, 1638, 1974
MluNI	TGGCCA	1 cut(s)	1147
Mly113I	GGCGCC	1 cut(s)	132
MlyI	GAGTC	2 cut(s)	830, 947
MmeI	TCCRAC	2 cut(s)	142, 595
Mox20I	TGGCCA	1 cut(s)	1147
Mph1103I	ATGCAT	3 cut(s)	666, 1160, 1519
MroI	TCCGGA	1 cut(s)	979
MroNI	GCCGGC	2 cut(s)	39, 167
MscI	TGGCCA	1 cut(s)	1147
MseI	TTAA	6 cut(s)	84, 246, 356, 756, 1401, 1559
MslI	CAYNNNNRTG	5 cut(s)	492, 1218, 1428, 1592, 2009
Msp20I	TGGCCA	1 cut(s)	1147
MspI	CCGG	5 cut(s)	40, 168, 980, 1414, 1775
MspR9I	CCNGG	5 cut(s)	1136, 1488, 1533, 1775, 1776
Mva1269I	GAATGC	4 cut(s)	471, 678, 1240, 2004
MvaI	CCWGG	3 cut(s)	1136, 1488, 1533
MvnI	CGCG	1 cut(s)	1273
NaeI	GCCGGC	2 cut(s)	41, 169
NarI	GGCGCC	1 cut(s)	132
NciI	CCSGG	2 cut(s)	1775, 1776
NcoI	CCATGG	1 cut(s)	2092
NdeI	CATATG	2 cut(s)	1261, 1335
NdeII	GATC	6 cut(s)	403, 559, 1102, 1695, 1799, 2034
NgoMIV	GCCGGC	2 cut(s)	39, 167
NheI	GCTAGC	1 cut(s)	11
NlaIV	GGNNCC	6 cut(s)	133, 166, 269, 1539, 1540, 1782
NmeAIII	GCCGAG	2 cut(s)	73, 126
NmuCI	GTSAC	4 cut(s)	365, 939, 1198, 2018
NsiI	ATGCAT	3 cut(s)	666, 1160, 1519
NspI	RCATGY	4 cut(s)	668, 707, 1158, 1427
PaeI	GCATGC	1 cut(s)	668
PaeR7I	CTCGAG	1 cut(s)	911
PagI	TCATGA	1 cut(s)	487
PciI	ACATGT	1 cut(s)	1423
PciSI	GCTCTTC	1 cut(s)	1868
PcsI	WCGNNNNNNNCGW	5 cut(s)	78, 93, 105, 114, 147
PctI	GAATGC	4 cut(s)	471, 678, 1240, 2004
PdiI	GCCGGC	2 cut(s)	41, 169
PfeI	GAWTC	1 cut(s)	929
PflFI	GACNNNGTC	1 cut(s)	1488
PflMI	CCANNNNNTGG	2 cut(s)	1664, 2093
PinAI	ACCGGT	1 cut(s)	1413
PkrI	GCNGC	5 cut(s)	197, 949, 1381, 1586, 1769
PleI	GAGTC	2 cut(s)	830, 946
PluTI	GGCGCC	1 cut(s)	135
PpsI	GAGTC	2 cut(s)	830, 946
PpuMI	RGGWCCY	1 cut(s)	1538
PscI	ACATGT	1 cut(s)	1423
Psp124BI	GAGCTC	1 cut(s)	156
Psp1406I	AACGTT	1 cut(s)	641
Psp5II	RGGWCCY	1 cut(s)	1538
Psp6I	CCWGG	3 cut(s)	1134, 1486, 1531
PspFI	CCCAGC	1 cut(s)	34
PspGI	CCWGG	3 cut(s)	1134, 1486, 1531
PspN4I	GGNNCC	6 cut(s)	133, 166, 269, 1539, 1540, 1782
PspPI	GGNCC	9 cut(s)	22, 37, 45, 347, 503, 1411, 1490, 1538, 1601
PspPPI	RGGWCCY	1 cut(s)	1538
PspXI	VCTCGAGB	1 cut(s)	911
PstI	CTGCAG	1 cut(s)	2127
PsyI	GACNNNGTC	1 cut(s)	1488
RsaI	GTAC	3 cut(s)	77, 628, 1853
RsaNI	GTAC	3 cut(s)	76, 627, 1852
RseI	CAYNNNNRTG	5 cut(s)	492, 1218, 1428, 1592, 2009
SacI	GAGCTC	1 cut(s)	156
SapI	GCTCTTC	1 cut(s)	1868
SaqAI	TTAA	6 cut(s)	84, 246, 356, 756, 1401, 1559
SatI	GCNGC	5 cut(s)	196, 948, 1380, 1585, 1768
Sau3AI	GATC	6 cut(s)	403, 559, 1102, 1695, 1799, 2034
Sau96I	GGNCC	9 cut(s)	22, 37, 45, 347, 503, 1411, 1490, 1538, 1601
SchI	GAGTC	2 cut(s)	830, 947
ScrFI	CCNGG	5 cut(s)	1136, 1488, 1533, 1775, 1776
SduI	GDGCHC	4 cut(s)	66, 156, 1051, 1938
SexAI	ACCWGGT	2 cut(s)	1134, 1486
SfcI	CTRYAG	1 cut(s)	2123
SfoI	GGCGCC	1 cut(s)	133
Sfr274I	CTCGAG	1 cut(s)	911
SinI	GGWCC	4 cut(s)	22, 1411, 1490, 1538
SlaI	CTCGAG	1 cut(s)	911
SmaI	CCCGGG	1 cut(s)	1776
SmiI	ATTTAAAT	1 cut(s)	1560
SmiMI	CAYNNNNRTG	5 cut(s)	492, 1218, 1428, 1592, 2009
SmlI	CTYRAG	1 cut(s)	911
SmoI	CTYRAG	1 cut(s)	911
SpeI	ACTAGT	1 cut(s)	1282
SphI	GCATGC	1 cut(s)	668
Sse9I	AATT	9 cut(s)	564, 1181, 1229, 1398, 1555, 1561, 1569, 1638, 1974
SsiI	CCGC	9 cut(s)	43, 196, 265, 962, 1024, 1273, 1360, 1585, 1913
SspDI	GGCGCC	1 cut(s)	131
SspI	AATATT	1 cut(s)	699
SspMI	CTAG	6 cut(s)	12, 684, 1124, 1176, 1283, 1730
SstI	GAGCTC	1 cut(s)	156
StyD4I	CCNGG	5 cut(s)	1134, 1486, 1531, 1773, 1774
StyI	CCWWGG	3 cut(s)	683, 1996, 2092
SwaI	ATTTAAAT	1 cut(s)	1560
TaaI	ACNGT	5 cut(s)	7, 125, 262, 1195, 1672
TaiI	ACGT	4 cut(s)	57, 90, 367, 644
TaqI	TCGA	5 cut(s)	57, 72, 108, 138, 912
TaqII	GACCGA	1 cut(s)	384
TasI	AATT	9 cut(s)	564, 1181, 1229, 1398, 1555, 1561, 1569, 1638, 1974
TatI	WGTACW	2 cut(s)	75, 1851
TauI	GCSGC	2 cut(s)	198, 1587
TfiI	GAWTC	1 cut(s)	929
Tru1I	TTAA	6 cut(s)	84, 246, 356, 756, 1401, 1559
Tru9I	TTAA	6 cut(s)	84, 246, 356, 756, 1401, 1559
TscAI	CASTG	6 cut(s)	24, 752, 766, 1207, 1516, 1831
TseFI	GTSAC	4 cut(s)	365, 939, 1198, 2018
TseI	GCWGC	3 cut(s)	947, 1379, 1767
Tsp45I	GTSAC	4 cut(s)	365, 939, 1198, 2018
TspDTI	ATGAA	5 cut(s)	504, 907, 1097, 1098, 1208
TspGWI	ACGGA	3 cut(s)	469, 512, 1740
TspMI	CCCGGG	1 cut(s)	1774
TspRI	CASTG	6 cut(s)	24, 752, 766, 1207, 1516, 1831
Tth111I	GACNNNGTC	1 cut(s)	1488
Van91I	CCANNNNNTGG	2 cut(s)	1664, 2093
VpaK11BI	GGWCC	4 cut(s)	22, 1411, 1490, 1538
XapI	RAATTY	3 cut(s)	1181, 1555, 1561
XceI	RCATGY	4 cut(s)	668, 707, 1158, 1427
XhoI	CTCGAG	1 cut(s)	911
XmaI	CCCGGG	1 cut(s)	1774
XmaJI	CCTAGG	1 cut(s)	683
XspI	CTAG	6 cut(s)	12, 684, 1124, 1176, 1283, 1730
ZraI	GACGTC	1 cut(s)	55
Zsp2I	ATGCAT	3 cut(s)	666, 1160, 1519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.