MD14G1171300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015127
Physical Location & Seq
Forward (+)
26524153 .. 26524581
429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1171300.v1.1.491

Sequence Viewer

Length: 429 bp
ATGAAACAAAAGTTACAGCTTGAAGATGTCGACAGAGAACTTGAAATCATCAAGGATGTGGCACAAGCTTGGTACAGCCACTCCGGCAACTCCCGGCCCATGTCCGAATTTGATGCCCACCGGAGAAACTACAAAGGTAAGTCACCGTCCCGGTTGAAGCTTGAAGCAATGAGAAAATCATCATCAGCTAGTGCACATCAGAGTGGTTATGAAAAATGGGATTTTGGGCAGTCACTATGGGATGCTTATGAGATTGTTGCCGTGTCGAAAAGGTTAGAGACCGGGCTAGTCCTTGATGAGTTGGAGAGTTCGGCTCGAGTCCATCGGAGACGTAGGGAGAGCAAGCAGAGTCTTATTAGAAGTTTTTTCAATACAATGTTTTCAAGGAGATTTAATGAGGCAGATATTCCACCTGATGAGGATACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

143

Amino Acids

16.61

Weight (kDa)

8.89

Isoelectric Point (pI)

67.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24700
fragaria_vesca FvH4_5g06310
malus_domestica MD06G1165700.v1.1 MD14G1171300.v1.1
prunus_persica Prupe.5G165400_v2.0.a1
pyrus_communis pycom06g14820
rosa_chinensis RchiOBHm_Chr7g0192551
rosa_laevigata RLG00000004353
rosa_multiflora Rmu_sc0003108.1_g000040
rosa_roxburghii Rroxscaffold_3G00262940
rosa_rugosa Rorug07G0002100
rosa_samantha Rh7AG126600 Rh7BG128800 Rh7CG130600 Rh7DG130100
rosa_wichuraiana Rw7G010880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 30
AcsI RAATTY 1 cut(s) 107
AfaI GTAC 1 cut(s) 74
AgsI TTSAA 6 cut(s) 23, 44, 157, 164, 370, 384
AluBI AGCT 4 cut(s) 19, 68, 160, 188
AluI AGCT 4 cut(s) 19, 68, 160, 188
Alw21I GWGCWC 1 cut(s) 196
Alw26I GTCTC 2 cut(s) 272, 322
Alw44I GTGCAC 1 cut(s) 192
Ama87I CYCGRG 1 cut(s) 315
AoxI GGCC 1 cut(s) 95
ApaLI GTGCAC 1 cut(s) 192
ApoI RAATTY 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 96
AsuC2I CCSGG 3 cut(s) 94, 151, 283
AsuHPI GGTGA 1 cut(s) 135
AvaI CYCGRG 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 196
Bbv12I GWGCWC 1 cut(s) 196
BccI CCATC 1 cut(s) 330
BceAI ACGGC 1 cut(s) 245
BcgI CGANNNNNNTGC 2 cut(s) 95, 129
BciVI GTATCC 1 cut(s) 415
BcnI CCSGG 3 cut(s) 94, 151, 283
BcoDI GTCTC 2 cut(s) 272, 322
BfaI CTAG 2 cut(s) 189, 287
BfuI GTATCC 1 cut(s) 415
BglI GCCNNNNNGGC 1 cut(s) 84
Bme1390I CCNGG 3 cut(s) 94, 151, 283
BmeT110I CYCGRG 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 96
BmrFI CCNGG 3 cut(s) 94, 151, 283
BmsI GCATC 2 cut(s) 103, 232
BplI GAGNNNNNCTC 2 cut(s) 298, 330
BpuMI CCSGG 3 cut(s) 94, 151, 283
BsaAI YACGTR 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 272
BsaWI WCCGGW 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bse3DI GCAATG 1 cut(s) 174
BseGI GGATG 2 cut(s) 61, 247
BseMI GCAATG 1 cut(s) 174
BseSI GKGCMC 1 cut(s) 196
BshFI GGCC 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 196
BsiHKCI CYCGRG 1 cut(s) 315
BsiSI CCGG 5 cut(s) 84, 94, 121, 151, 282
BslFI GGGAC 1 cut(s) 133
BsmAI GTCTC 2 cut(s) 272, 322
BsmBI CGTCTC 1 cut(s) 322
BsmFI GGGAC 1 cut(s) 133
BsnI GGCC 1 cut(s) 97
Bso31I GGTCTC 1 cut(s) 272
BsoBI CYCGRG 1 cut(s) 315
Bsp1286I GDGCHC 1 cut(s) 196
BspANI GGCC 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 174
Bst4CI ACNGT 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 344
BstF5I GGATG 2 cut(s) 61, 247
BstMAI GTCTC 2 cut(s) 272, 322
BstMWI GCNNNNNNNGC 1 cut(s) 84
BstSCI CCNGG 3 cut(s) 92, 149, 281
BstSLI GKGCMC 1 cut(s) 196
BstSNI TACGTA 1 cut(s) 426
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 1 cut(s) 97
BtsCI GGATG 2 cut(s) 61, 247
Cac8I GCNNGC 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 96
Csp6I GTAC 1 cut(s) 73
CviAII CATG 1 cut(s) 100
CviJI RGCY 8 cut(s) 19, 68, 78, 97, 160, 188, 286, 314
CviKI_1 RGCY 8 cut(s) 19, 68, 78, 97, 160, 188, 286, 314
CviQI GTAC 1 cut(s) 73
Eco105I TACGTA 1 cut(s) 426
Eco31I GGTCTC 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 1 cut(s) 103
FaiI YATR 4 cut(s) 101, 210, 238, 249
FaqI GGGAC 1 cut(s) 133
FatI CATG 1 cut(s) 99
FblI GTMKAC 1 cut(s) 30
FokI GGATG 2 cut(s) 68, 254
FspBI CTAG 2 cut(s) 189, 287
HaeIII GGCC 1 cut(s) 97
HapII CCGG 5 cut(s) 84, 94, 121, 151, 282
Hin1II CATG 1 cut(s) 103
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 2 cut(s) 66, 158
HinfI GANTC 2 cut(s) 318, 349
HpaII CCGG 5 cut(s) 84, 94, 121, 151, 282
HphI GGTGA 1 cut(s) 135
Hpy166II GTNNAC 2 cut(s) 31, 194
Hpy188I TCNGA 3 cut(s) 106, 201, 327
Hpy8I GTNNAC 2 cut(s) 31, 194
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4IV ACGT 2 cut(s) 331, 425
HpyCH4V TGCA 1 cut(s) 194
HpyF10VI GCNNNNNNNGC 1 cut(s) 84
HpySE526I ACGT 2 cut(s) 331, 425
Hsp92II CATG 1 cut(s) 103
LpnPI CCDG 5 cut(s) 97, 107, 134, 164, 295
LweI GCATC 2 cut(s) 103, 232
MaeI CTAG 2 cut(s) 189, 287
MaeII ACGT 2 cut(s) 331, 425
MaeIII GTNAC 3 cut(s) 12, 141, 231
MboII GAAGA 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 196
MluCI AATT 1 cut(s) 107
MlyI GAGTC 2 cut(s) 327, 358
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 2 cut(s) 391, 412
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 201
MspI CCGG 5 cut(s) 84, 94, 121, 151, 282
MspR9I CCNGG 3 cut(s) 94, 151, 283
MwoI GCNNNNNNNGC 1 cut(s) 84
NciI CCSGG 3 cut(s) 94, 151, 283
NlaIII CATG 1 cut(s) 103
NmuCI GTSAC 2 cut(s) 141, 231
PaeR7I CTCGAG 1 cut(s) 315
PleI GAGTC 2 cut(s) 326, 357
PpsI GAGTC 2 cut(s) 326, 357
Ppu21I YACGTR 1 cut(s) 426
PspPI GGNCC 1 cut(s) 96
PspXI VCTCGAGB 1 cut(s) 315
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 1 cut(s) 201
SalI GTCGAC 1 cut(s) 29
SaqAI TTAA 1 cut(s) 393
Sau96I GGNCC 1 cut(s) 96
SchI GAGTC 2 cut(s) 327, 358
ScrFI CCNGG 3 cut(s) 94, 151, 283
SduI GDGCHC 1 cut(s) 196
SetI ASST 9 cut(s) 21, 70, 139, 162, 190, 275, 334, 415, 428
SfaNI GCATC 2 cut(s) 103, 232
Sfr274I CTCGAG 1 cut(s) 315
SlaI CTCGAG 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 201
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
SnaBI TACGTA 1 cut(s) 426
Sse9I AATT 1 cut(s) 107
SspMI CTAG 2 cut(s) 189, 287
StyD4I CCNGG 3 cut(s) 92, 149, 281
TaaI ACNGT 1 cut(s) 147
TaiI ACGT 2 cut(s) 334, 428
TaqI TCGA 3 cut(s) 30, 266, 316
TasI AATT 1 cut(s) 107
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TseFI GTSAC 2 cut(s) 141, 231
Tsp45I GTSAC 2 cut(s) 141, 231
TspDTI ATGAA 2 cut(s) 17, 225
VneI GTGCAC 1 cut(s) 192
XapI RAATTY 1 cut(s) 107
XhoI CTCGAG 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 30
XspI CTAG 2 cut(s) 189, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.