MD14G1172000.v1.1

Anaphase-promoting complex subunit 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015257
Physical Location & Seq
Forward (+)
26569760 .. 26571789
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1172000.v1.1.491

Sequence Viewer

Length: 300 bp
ATGTTACAGTTCCCGGGGTTCATGAAACAGTACCCGTGGTCAACAAGGACCATACCGACGTCGTTTCTGCTGCCAACCCAATGGCCCCAACCCCATAGTGAAGAGCTCCTCCTCGCCATGGAGGAGTCCGATTTCGAAGAGAAGTGCAATGAAATCAGAAAGAGTAGCAGCAGCATGGCTGTTATTGGGAAAACCGCTGTCGACAACGATAAAGAAGACTATGACAACGATCCAGATGATGATGATGTAGACAATGCAGAGGATTCCGAAGGTGAAGAGTTTGAGCAGGAAACCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC15

Protein Analysis

100

Amino Acids

11.39

Weight (kDa)

4.05

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC15 PF15243 8 - 97 5.6e-18 Anaphase-promoting complex subunit 15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63135
fragaria_vesca FvH4_5g06250
malus_domestica MD14G1172000.v1.1
prunus_persica Prupe.5G166100_v2.0.a1
pyrus_communis pycom06g14870
rosa_chinensis RchiOBHm_Chr7g0192611
rosa_multiflora Rmu_sc0004161.1_g000003
rosa_roxburghii Rroxscaffold_3G00262840
rosa_rugosa Rorug07G0002900
rosa_samantha Rh2CG350000 Rh7AG127100 Rh7CG131200 Rh7DG130700
rosa_wichuraiana Rw2G014800 Rw6G012180 Rw7G010950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 62
AccI GTMKAC 2 cut(s) 201, 249
AciI CCGC 1 cut(s) 195
AclWI GGATC 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 1 cut(s) 118
AluBI AGCT 1 cut(s) 106
AluI AGCT 1 cut(s) 106
Alw21I GWGCWC 1 cut(s) 108
AlwI GGATC 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 70, 168, 171
AspS9I GGNCC 2 cut(s) 48, 84
AsuC2I CCSGG 2 cut(s) 14, 15
AsuHPI GGTGA 1 cut(s) 284
AsuII TTCGAA 1 cut(s) 135
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 1 cut(s) 48
BanII GRGCYC 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 3 cut(s) 57, 180, 183
BcnI CCSGG 2 cut(s) 14, 15
BisI GCNGC 3 cut(s) 71, 169, 172
BlsI GCNGC 3 cut(s) 72, 170, 173
Bme1390I CCNGG 2 cut(s) 14, 15
Bme18I GGWCC 1 cut(s) 48
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 2 cut(s) 48, 84
BmiI GGNNCC 1 cut(s) 86
BmrFI CCNGG 2 cut(s) 14, 15
BpiI GAAGAC 1 cut(s) 222
Bpu14I TTCGAA 1 cut(s) 135
BpuMI CCSGG 2 cut(s) 14, 15
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 4 cut(s) 13, 14, 35, 117
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 1 cut(s) 154
BseDI CCNNGG 4 cut(s) 13, 14, 35, 117
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 154
BseNI ACTGG 1 cut(s) 294
BseRI GAGGAG 3 cut(s) 98, 101, 137
BseXI GCAGC 3 cut(s) 57, 180, 183
BshFI GGCC 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 108
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 1 cut(s) 14
BslI CCNNNNNNNGG 1 cut(s) 118
BsnI GGCC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 13
Bsp119I TTCGAA 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 1 cut(s) 229
Bsp19I CCATGG 1 cut(s) 117
BspACI CCGC 1 cut(s) 195
BspANI GGCC 1 cut(s) 85
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 86
BspPI GGATC 1 cut(s) 224
BspQI GCTCTTC 1 cut(s) 96
BspT104I TTCGAA 1 cut(s) 135
BsrDI GCAATG 1 cut(s) 154
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 4 cut(s) 13, 14, 35, 117
BssMI GATC 1 cut(s) 229
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 9, 30
Bst6I CTCTTC 3 cut(s) 96, 132, 270
BstACI GRCGYC 1 cut(s) 59
BstBI TTCGAA 1 cut(s) 135
BstDSI CCRYGG 2 cut(s) 35, 117
BstKTI GATC 1 cut(s) 232
BstMBI GATC 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 12, 13
BstV1I GCAGC 3 cut(s) 57, 180, 183
BstV2I GAAGAC 1 cut(s) 222
BstXI CCANNNNNNTGG 1 cut(s) 81
BsuRI GGCC 1 cut(s) 85
BtgI CCRYGG 2 cut(s) 35, 117
CciI TCATGA 1 cut(s) 21
Cfr13I GGNCC 2 cut(s) 48, 84
Cfr9I CCCGGG 1 cut(s) 13
Csp6I GTAC 1 cut(s) 31
CviAII CATG 3 cut(s) 22, 118, 175
CviJI RGCY 3 cut(s) 85, 106, 179
CviKI_1 RGCY 3 cut(s) 85, 106, 179
CviQI GTAC 1 cut(s) 31
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
Eam1104I CTCTTC 3 cut(s) 96, 132, 270
EarI CTCTTC 3 cut(s) 96, 132, 270
Ecl136II GAGCTC 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 106
Eco88I CYCGRG 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 117
EcoT38I GRGCYC 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 3 cut(s) 25, 121, 178
FaiI YATR 6 cut(s) 23, 53, 96, 119, 176, 222
FatI CATG 3 cut(s) 21, 117, 174
FblI GTMKAC 2 cut(s) 201, 249
Fnu4HI GCNGC 3 cut(s) 71, 169, 172
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 3 cut(s) 71, 169, 172
GluI GCNGC 3 cut(s) 71, 169, 172
HaeIII GGCC 1 cut(s) 85
HapII CCGG 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 3 cut(s) 25, 121, 178
HincII GTYRAC 2 cut(s) 42, 202
HindII GTYRAC 2 cut(s) 42, 202
HinfI GANTC 2 cut(s) 125, 263
HpaII CCGG 1 cut(s) 14
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 3 cut(s) 42, 202, 250
Hpy188I TCNGA 3 cut(s) 130, 158, 268
Hpy188III TCNNGA 2 cut(s) 22, 233
Hpy8I GTNNAC 3 cut(s) 42, 202, 250
Hpy99I CGWCG 2 cut(s) 61, 64
HpyAV CCTTC 1 cut(s) 263
HpyCH4III ACNGT 2 cut(s) 9, 30
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 147, 257
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 3 cut(s) 25, 121, 178
Kzo9I GATC 1 cut(s) 229
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 1 cut(s) 111
LpnPI CCDG 3 cut(s) 27, 246, 272
Lsp1109I GCAGC 3 cut(s) 57, 180, 183
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 1 cut(s) 3
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 4 cut(s) 113, 149, 227, 287
MhlI GDGCHC 1 cut(s) 108
MlyI GAGTC 1 cut(s) 134
MnlI CCTC 4 cut(s) 115, 119, 122, 253
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 2 cut(s) 14, 15
NciI CCSGG 2 cut(s) 14, 15
NcoI CCATGG 1 cut(s) 117
NdeII GATC 1 cut(s) 229
NlaIII CATG 3 cut(s) 25, 121, 178
NlaIV GGNNCC 1 cut(s) 86
NspV TTCGAA 1 cut(s) 135
PagI TCATGA 1 cut(s) 21
PciSI GCTCTTC 1 cut(s) 96
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 3 cut(s) 72, 170, 173
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Psp124BI GAGCTC 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 86
PspPI GGNCC 2 cut(s) 48, 84
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SacI GAGCTC 1 cut(s) 108
SalI GTCGAC 1 cut(s) 200
SapI GCTCTTC 1 cut(s) 96
SatI GCNGC 3 cut(s) 71, 169, 172
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 2 cut(s) 48, 84
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 2 cut(s) 14, 15
SduI GDGCHC 1 cut(s) 108
SetI ASST 3 cut(s) 62, 108, 274
SfuI TTCGAA 1 cut(s) 135
SinI GGWCC 1 cut(s) 48
SmaI CCCGGG 1 cut(s) 15
SsiI CCGC 1 cut(s) 195
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 12, 13
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 2 cut(s) 9, 30
TaiI ACGT 1 cut(s) 62
TaqI TCGA 2 cut(s) 135, 201
TfiI GAWTC 1 cut(s) 263
TseI GCWGC 3 cut(s) 70, 168, 171
TspDTI ATGAA 3 cut(s) 10, 38, 165
TspMI CCCGGG 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 48
XmaI CCCGGG 1 cut(s) 13
XmiI GTMKAC 2 cut(s) 201, 249
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.