MD14G1173100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0017088
Physical Location & Seq
Forward (+)
26714351 .. 26714911
561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1173100.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGCACCCCCCGAGGATGTCAAAATCCTCGCCAAATTCAAGTCCATGCACAACTACATTCGGGTCCTCGAGGTCTCGAAAAGGGCTGACGACCCCTTTACCGGCTCAAGACTCCTCCTTCTTGACAACCCTGGAAACATCCACAGCATCTCTTTCATTTTCAAGTCCCTCACAAGCACTTACTTCGACGTCTTCACCTTTCCGCCGATTATCCCTCCCGGACCCATAGGCATTCTTGGGTTCGGGGCGGGATCCGTGGCAAGGTCGATTCTTGATCTGTACCCAGAAGTTGTGGTTCATGGGTGGGAGCTTGACCCATCTGTGATTGGTGTGGGCAGAGAGTACTCTGGACTCTCAAAGCTTGAGAGGCAATACACAGATAGGCTTATAATTCATGTCGGAGATGCCTTCAAAGTCAGCACTCGAGGAGGTAGCTGTGTGGAGGCTGAAGATTCACTGAGACATGGGAAATTGGTTACGGAAGAAACTCTTGAAGGAAATGCAACGGGTTTTTGGAGACAAGCTTTTCGTGTTGAGTCTTGGGAATCGATAGGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.62

Weight (kDa)

5.96

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63100
fragaria_vesca FvH4_5g06050
malus_domestica MD06G1167600.v1.1 MD14G1173100.v1.1
rosa_chinensis RchiOBHm_Chr7g0192811
rosa_laevigata RLG00000004327
rosa_multiflora Rmu_sc0002040.1_g000029
rosa_roxburghii Rroxscaffold_3G00262670
rosa_rugosa Rorug07G0004600
rosa_samantha Rh7AG130000 Rh7CG134400 Rh7DG132200
rosa_wichuraiana Rw7G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AatII GACGTC 1 cut(s) 192
AbsI CCTCGAGG 1 cut(s) 68
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 2 cut(s) 203, 248
AclWI GGATC 2 cut(s) 246, 259
AcsI RAATTY 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 2 cut(s) 281, 344
AfiI CCNNNNNNNGG 2 cut(s) 101, 261
AgsI TTSAA 4 cut(s) 40, 163, 412, 494
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 4 cut(s) 310, 361, 435, 524
AluI AGCT 4 cut(s) 310, 361, 435, 524
Alw26I GTCTC 3 cut(s) 79, 454, 511
AlwI GGATC 2 cut(s) 246, 259
Ama87I CYCGRG 3 cut(s) 11, 68, 423
ApoI RAATTY 1 cut(s) 35
ArsI GACNNNNNNTTYG 2 cut(s) 510, 542
AspS9I GGNCC 2 cut(s) 64, 221
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 1 cut(s) 187
AvaI CYCGRG 3 cut(s) 11, 68, 423
AvaII GGWCC 2 cut(s) 64, 221
BamHI GGATCC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 184
BccI CCATC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 1 cut(s) 132
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 3 cut(s) 79, 454, 511
BmcAI AGTACT 1 cut(s) 344
Bme1390I CCNGG 2 cut(s) 132, 219
Bme18I GGWCC 2 cut(s) 64, 221
BmeT110I CYCGRG 3 cut(s) 11, 68, 423
BmgT120I GGNCC 2 cut(s) 64, 221
BmiI GGNNCC 4 cut(s) 6, 65, 223, 253
BmrFI CCNGG 2 cut(s) 132, 219
BmsI GCATC 2 cut(s) 156, 394
BpiI GAAGAC 1 cut(s) 184
BpuEI CTTGAG 2 cut(s) 91, 383
BpuMI CCSGG 1 cut(s) 219
Bsa29I ATCGAT 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 79
BsaJI CCNNGG 3 cut(s) 12, 130, 255
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 261
Bse118I RCCGGY 1 cut(s) 101
BseBI CCWGG 1 cut(s) 132
BseCI ATCGAT 1 cut(s) 548
BseDI CCNNGG 3 cut(s) 12, 130, 255
BseGI GGATG 2 cut(s) 22, 138
BseLI CCNNNNNNNGG 2 cut(s) 101, 261
BseMII CTCAG 1 cut(s) 449
BseRI GAGGAG 2 cut(s) 104, 441
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 1 cut(s) 548
BsiHKCI CYCGRG 3 cut(s) 11, 68, 423
BsiSI CCGG 2 cut(s) 102, 219
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 2 cut(s) 101, 261
BsmAI GTCTC 3 cut(s) 79, 454, 511
BsmFI GGGAC 1 cut(s) 151
BsmI GAATGC 1 cut(s) 231
Bso31I GGTCTC 1 cut(s) 79
BsoBI CYCGRG 3 cut(s) 11, 68, 423
Bsp143I GATC 2 cut(s) 251, 274
BspACI CCGC 2 cut(s) 203, 248
BspCNI CTCAG 1 cut(s) 450
BspDI ATCGAT 1 cut(s) 548
BspLI GGNNCC 4 cut(s) 6, 65, 223, 253
BspPI GGATC 2 cut(s) 246, 259
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 79
BsrFI RCCGGY 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 3 cut(s) 12, 130, 255
BssMI GATC 2 cut(s) 251, 274
BssNI GRCGYC 1 cut(s) 189
Bst2UI CCWGG 1 cut(s) 132
BstACI GRCGYC 1 cut(s) 189
BstDEI CTNAG 1 cut(s) 458
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 2 cut(s) 22, 138
BstKTI GATC 2 cut(s) 254, 277
BstMAI GTCTC 3 cut(s) 79, 454, 511
BstMBI GATC 2 cut(s) 251, 274
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 130, 217
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
Bsu15I ATCGAT 1 cut(s) 548
BsuTUI ATCGAT 1 cut(s) 548
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 2 cut(s) 22, 138
BtsIMutI CAGTG 1 cut(s) 455
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 2 cut(s) 64, 221
ClaI ATCGAT 1 cut(s) 548
Csp6I GTAC 2 cut(s) 280, 343
CviAII CATG 4 cut(s) 46, 299, 395, 464
CviJI RGCY 8 cut(s) 86, 105, 310, 361, 385, 435, 446, 524
CviKI_1 RGCY 8 cut(s) 86, 105, 310, 361, 385, 435, 446, 524
CviQI GTAC 2 cut(s) 280, 343
DdeI CTNAG 1 cut(s) 458
DpnI GATC 2 cut(s) 253, 276
DpnII GATC 2 cut(s) 251, 274
EciI GGCGGA 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 79
Eco47I GGWCC 2 cut(s) 64, 221
Eco57I CTGAAG 1 cut(s) 468
Eco88I CYCGRG 3 cut(s) 11, 68, 423
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 130
FaeI CATG 4 cut(s) 49, 302, 398, 467
FaiI YATR 6 cut(s) 47, 227, 300, 389, 396, 465
FalI AAGNNNNNCTT 2 cut(s) 474, 506
FaqI GGGAC 1 cut(s) 151
FatI CATG 4 cut(s) 45, 298, 394, 463
FauI CCCGC 1 cut(s) 241
FokI GGATG 2 cut(s) 29, 125
HapII CCGG 2 cut(s) 102, 219
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 4 cut(s) 49, 302, 398, 467
HindIII AAGCTT 2 cut(s) 359, 522
HinfI GANTC 6 cut(s) 111, 268, 351, 452, 536, 545
HpaII CCGG 2 cut(s) 102, 219
HphI GGTGA 1 cut(s) 187
Hpy188I TCNGA 1 cut(s) 401
Hpy188III TCNNGA 6 cut(s) 76, 108, 122, 272, 348, 491
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 128, 418, 488
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 2 cut(s) 49, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 1 cut(s) 458
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 4 cut(s) 49, 302, 398, 467
Kzo9I GATC 2 cut(s) 251, 274
LmnI GCTCC 1 cut(s) 307
LpnPI CCDG 6 cut(s) 115, 117, 144, 232, 297, 333
LweI GCATC 2 cut(s) 156, 394
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 475
MalI GATC 2 cut(s) 253, 276
MboI GATC 2 cut(s) 251, 274
MboII GAAGA 3 cut(s) 184, 461, 494
MflI RGATCY 1 cut(s) 251
MluCI AATT 3 cut(s) 35, 390, 470
MlyI GAGTC 3 cut(s) 105, 345, 545
MmeI TCCRAC 1 cut(s) 379
MspI CCGG 2 cut(s) 102, 219
MspR9I CCNGG 2 cut(s) 132, 219
Mva1269I GAATGC 1 cut(s) 231
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 367
NciI CCSGG 1 cut(s) 219
NdeII GATC 2 cut(s) 251, 274
NlaIII CATG 4 cut(s) 49, 302, 398, 467
NlaIV GGNNCC 4 cut(s) 6, 65, 223, 253
PaeR7I CTCGAG 2 cut(s) 68, 423
PctI GAATGC 1 cut(s) 231
PfeI GAWTC 3 cut(s) 268, 452, 545
PfoI TCCNGGA 1 cut(s) 217
PleI GAGTC 3 cut(s) 105, 345, 544
PpsI GAGTC 3 cut(s) 105, 345, 544
PpuMI RGGWCCY 1 cut(s) 64
PsiI TTATAA 1 cut(s) 389
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 4 cut(s) 6, 65, 223, 253
PspPI GGNCC 2 cut(s) 64, 221
PspPPI RGGWCCY 1 cut(s) 64
PspXI VCTCGAGB 2 cut(s) 68, 423
PsuI RGATCY 1 cut(s) 251
RsaI GTAC 2 cut(s) 281, 344
RsaNI GTAC 2 cut(s) 280, 343
Sau3AI GATC 2 cut(s) 251, 274
Sau96I GGNCC 2 cut(s) 64, 221
ScaI AGTACT 1 cut(s) 344
SchI GAGTC 3 cut(s) 105, 345, 545
ScrFI CCNGG 2 cut(s) 132, 219
SetI ASST 9 cut(s) 75, 192, 200, 266, 312, 363, 433, 437, 526
SfaNI GCATC 2 cut(s) 156, 394
Sfr274I CTCGAG 2 cut(s) 68, 423
SinI GGWCC 2 cut(s) 64, 221
SlaI CTCGAG 2 cut(s) 68, 423
SmlI CTYRAG 4 cut(s) 68, 106, 362, 423
SmoI CTYRAG 4 cut(s) 68, 106, 362, 423
Sse9I AATT 3 cut(s) 35, 390, 470
SsiI CCGC 2 cut(s) 203, 248
StyD4I CCNGG 2 cut(s) 130, 217
TaiI ACGT 1 cut(s) 192
TaqI TCGA 6 cut(s) 69, 77, 186, 266, 424, 548
TasI AATT 3 cut(s) 35, 390, 470
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 3 cut(s) 268, 452, 545
TscAI CASTG 1 cut(s) 462
TspDTI ATGAA 3 cut(s) 145, 287, 383
TspGWI ACGGA 2 cut(s) 244, 494
TspRI CASTG 1 cut(s) 462
VpaK11BI GGWCC 2 cut(s) 64, 221
XapI RAATTY 1 cut(s) 35
XhoI CTCGAG 2 cut(s) 68, 423
ZraI GACGTC 1 cut(s) 190
ZrmI AGTACT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.