Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004805

Physical Location & Seq

Genomic Coordinates Forward (+)

27248450 .. 27257075

Gene Structure 8626 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1179800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2724 bp

ATGGCTGGTTTTGGAACTAACGATATAAAAGGGAGAGAGAAGGAAGAAGGTGACAAAAACAGTAATACTTTAGAGTCCCATGTGGTGATTAGAACAGAAGGAGGAGAGGTTGGTCAAAGTAGTAGTGAGAGAGATACGATTCACCGGTCAGGTTCCCGGCCTCAGTTGGACCTCAGCAAAGCTGCCATTCAAGGGAATTTTGAAGAGAGGGACCCCGCAATTCTTCTGCCTAATCAGTCTGATGACTTGTCTCACTTGGCTTTGGACATTGGAGGATCTCTCATCAAGTTGGTGTATTTCTCAAGACATGAAGGCCAATCAATTGATGATAAAAGGAAGAAAACTTTGAAGGAAAGGTTGGGAATATCAAATGGTAGTAGGAGAAGCTACCCTATTCTAGGTGGGCGGCTTCATTTTGTGAAGTTTGAGACAAACAAGATTAACGAGTGCTTAGACTTCATCTATTCCAAGCAGCTTCACCGTGGTGGAACGGATGCTCGTATCTGGAATCCTGATGCCCCAACCAATGAAAGTTCTGTAATCAAGGCTACAGGTGGTGGGGCATACAAGTTTGCGGACCTCTTCAAAGAACGGCTTGGGGTTAGTCTTGACAAAGAAGACGAAATGAATTGTCTAGTATCTGGAGCAAATTTTCTCCTCAAGGCAATACGTCATGAAGCTTTCACACACATGGAGGGTCAGAAAGAGTTTGTGCAGATTGACCAGAATGAATTATTCCCATATCTTCTAGTTAACATTGGGTCTGGTGTTAGTATAATTAAGGTTGATGGAGATGGCAAATTTCAGCGGGTAAGTGGAACAAATGTTGGTGGCGGTACTTATTGGGGCTTGGGAAAACTGTTAACAAAGTGCAAAAGTTTCGATGAGTTGTTAGAGCTGAGTCAACGGGGAGACAATGGTGCTATAGACATGCTTGTCGGGGACATTTATGGTGGTATGGACTACTCAAAGATCGGTCTATCTGCATCAACCATCGCCTCTAGTTTTGGGAAGGCTATTTCAGAAAACAAGGAACTTGAAGACTACAGCCCTGAAGATATATCACTGTCTCTCTTACGAATGATTTCTTATAATATTGGCCAGATAGCTTATCTAAATGCACTACGGTTTGGTCTGAAGCGAATATATTTTGGAGGTTTTTTCATAAGGGGCCATGCTTATACCATGGACACTATATCCTTTGCGGTTCAGTTCTGGTCAAATGGAGATGCACAAGCAATGTTTTTGAGACATGAAGGTTTTCTGGGAGCTTTAGGTGCATTTATGAGCTATGAAAAGCATGGTCTTGATGACTTGAAGGTCCATCATTTAGTTGAAAGATTTCCTATGGGCGCACCATATACTGGAGGAAAGATTCATGGTCCACCACTTGGGGATTTGAACGAGAAGATTTCATGGATGGAGAAGTTTGTTCGGAAAGGGACTGAGATTACTGCTCCAGTACCAATGGCTCCTCCTGGAACTACCGGACTTGGAGGCTTTGAAGTTCCTTCGTCCAAAGGAGGTACTCTGCGTTCTGATGCAAGTGCTTTAAACATTGGGGTCCTACATCTGGTACCCACGTTGGAAGTCTTTCCATTATTGGCGGACCCAAAAACGTATGAGCCTAACACTATTGATCTCTCAGATCCCGGCGAGTTAGAGTACTGGTTCACTGTGCTGTCAGAGCATCTACCAGACCTCGTTGATAAGGCAGTGGCAAGTGAAGGTGGAACGGATGATGCTAAAAGAAGGGGCGATGCTTTTGCTCGTGCTTTTTCTGCTCACTTGGCACGGTTGATGGAGGAGCCTGCTGCATATGGAAAGTTAGGGTTGGTGAATCTATTGGAACTAAGAGAAGAATGCTTGAGGGAGTTCCAATTTGTTGACGCGTATAGAAGTATAAAACAAAGGGAAAATGAGGCATCACTTGCTGTTTTAGCCGATCTGCTGATGGAGCTTGATAGTATGAGTGAGGAAGCAAGACTACTTACCCTTATTGAAGGTGTCCTTGCTGCAAACATTTTCGACTGGGGATCCCGTGCCTGCGTGGATCTCTATCACAAAGGAACGATTATTGAAATTTATAGAATGAGCCGCAATAAGATGCAAAGACCTTGGCGCGTGGATGACTTTGATGTTTTCAAAGAGAGAATGTTAGGATCTGGAGATACAAAACCTCGTCCGCATAAAAGAGCTTTGCTATTTGTGGATAACTCGGGTGCTGACGTTATTCTAGGAATGCTGCCCCTAGCACGGGAACTTCTCCGACGTGGAACGGAAGTTGTTTTGGTTGCCAACTCCCTTCCGGCTCTAAATGATGCAACTGCAATGGAGCTTCCTGAAATTGTTGCTGAAGCTGCCAAGCACTGTGACATTCTTCGCAGAGCTGCTGAAGCGGGAGGTTTACTTGTCGATGCAATGGTTAGCTCTCTAGATGGTTCCAAGGGAAATTCATCTTCAGTTCCGTTAATGGTTGTTGAGAATGGTTGCGGCAGTCCTTGTATAGACTTACGGCAGGTTAGCTCTGAGCTAGCCGCTGCTGCAAAAGAGGCCGATTTGGTTATCTTGGAAGGGATGGGCAGGGCTCTTCATACCAACCTTAATGCGAAGTTTAAATGTGATGCGTTAAAGCTTGCAATGGTGAAGAATCAAAGGCTGGCCCAAAAACTGATCCAAGGCAACATATACGACTGTGTTTGCAGATACGAACCAGTAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004594 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0044237 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00770 ko01100 map00770 map01100

Pfam Domains

Protein Families

DUF89 Fumble

Protein Analysis

908

Amino Acids

99.62

Weight (kDa)

5.63

Isoelectric Point (pI)

36.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fumble	PF03630	85 - 429	2.7e-137	Fumble
ARMT1-like_dom	PF01937	566 - 891	1.3e-44	Damage-control phosphatase ARMT1-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004805)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32180 AT4G32180 AT4G32180
fragaria_vesca	FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350
malus_domestica	MD06G1173300.v1.1 MD14G1179800.v1.1
prunus_persica	Prupe.5G172800_v2.0.a1 Prupe.5G172800_v2.0.a1
pyrus_communis	pycom06g15440 pycom14g14960
rosa_chinensis	RchiOBHm_Chr7g0193481
rosa_laevigata	RLG00000004267
rosa_multiflora	Rmu_sc0006629.1_g000003 Rmu_sc0012783.1_g000004 Rmu_sc0031135.1_g000001
rosa_roxburghii	Rroxscaffold_3G00262060
rosa_rugosa	Rorug07G0009700 Rorug07G0009700
rosa_samantha	Rh7AG136400 Rh7BG136800 Rh7CG140200 Rh7DG138000
rosa_wichuraiana	Rw7G011630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1092
AasI	GACNNNNNNGTC	2 cut(s)	935, 1319
Acc36I	ACCTGC	1 cut(s)	2509
Acc65I	GGTACC	1 cut(s)	1576
AccB1I	GGYRCC	1 cut(s)	1576
AccB7I	CCANNNNNTGG	2 cut(s)	1364, 1391
AccII	CGCG	2 cut(s)	1892, 2124
AclWI	GGATC	7 cut(s)	283, 1643, 2031, 2044, 2061, 2170, 2668
AcoI	YGGCCR	1 cut(s)	1099
AcsI	RAATTY	5 cut(s)	196, 649, 800, 2082, 2452
AcuI	CTGAAG	5 cut(s)	1074, 1157, 2376, 2415, 2445
AfaI	GTAC	5 cut(s)	838, 1464, 1528, 1578, 1667
AfiI	CCNNNNNNNGG	7 cut(s)	192, 398, 1364, 1391, 1573, 2256, 2257
AflIII	ACRYGT	1 cut(s)	1890
AgeI	ACCGGT	1 cut(s)	144
AjiI	CACGTC	1 cut(s)	2273
AjnI	CCWGG	1 cut(s)	1477
AjuI	GAANNNNNNNTTGG	2 cut(s)	341, 373
AleI	CACNNNNGTG	1 cut(s)	483
Alw26I	GTCTC	5 cut(s)	255, 422, 906, 1074, 1243
AlwI	GGATC	7 cut(s)	283, 1643, 2031, 2044, 2061, 2170, 2668
Ama87I	CYCGRG	1 cut(s)	2218
AoxI	GGCC	6 cut(s)	158, 313, 1099, 1171, 2553, 2661
ApeKI	GCWGC	9 cut(s)	182, 472, 1814, 2015, 2245, 2360, 2390, 2540, 2543
ApoI	RAATTY	5 cut(s)	196, 649, 800, 2082, 2452
AsiGI	ACCGGT	1 cut(s)	144
Asp700I	GAANNNNTTC	4 cut(s)	1084, 1260, 1341, 1593
Asp718I	GGTACC	1 cut(s)	1576
AspLEI	GCGC	2 cut(s)	1355, 2124
AspS9I	GGNCC	9 cut(s)	169, 211, 577, 1171, 1321, 1382, 1564, 1609, 2662
AsuC2I	CCSGG	2 cut(s)	157, 1653
AsuHPI	GGTGA	6 cut(s)	62, 97, 134, 470, 1849, 2656
AsuNHI	GCTAGC	1 cut(s)	2533
AvaI	CYCGRG	1 cut(s)	2218
AvaII	GGWCC	7 cut(s)	169, 211, 577, 1321, 1382, 1564, 1609
BaeI	ACNNNNGTAYC	2 cut(s)	1568, 1601
BalI	TGGCCA	1 cut(s)	1101
BamHI	GGATCC	1 cut(s)	2036
BanI	GGYRCC	1 cut(s)	1576
BanII	GRGCYC	1 cut(s)	2590
BarI	GAAGNNNNNNTAC	2 cut(s)	1971, 2003
BauI	CACGAG	1 cut(s)	1770
BbsI	GAAGAC	2 cut(s)	624, 1047
BbvCI	CCTCAGC	1 cut(s)	173
BbvI	GCAGC	9 cut(s)	169, 484, 1801, 2002, 2232, 2347, 2377, 2527, 2530
BccI	CCATC	9 cut(s)	782, 788, 1001, 1332, 1414, 1795, 1948, 2432, 2572
BceAI	ACGGC	2 cut(s)	608, 2531
BcgI	CGANNNNNNTGC	4 cut(s)	862, 896, 1748, 1782
BciT130I	CCWGG	1 cut(s)	1479
BcnI	CCSGG	2 cut(s)	157, 1653
BcoDI	GTCTC	5 cut(s)	255, 422, 906, 1074, 1243
BfaI	CTAG	8 cut(s)	398, 635, 749, 1002, 2237, 2252, 2435, 2534
BfmI	CTRYAG	3 cut(s)	549, 924, 1045
BfuAI	ACCTGC	1 cut(s)	2509
BmcAI	AGTACT	1 cut(s)	1667
Bme1390I	CCNGG	3 cut(s)	157, 1479, 1653
Bme18I	GGWCC	7 cut(s)	169, 211, 577, 1321, 1382, 1564, 1609
BmeT110I	CYCGRG	1 cut(s)	2218
BmgBI	CACGTC	1 cut(s)	2273
BmgT120I	GGNCC	9 cut(s)	169, 211, 577, 1171, 1321, 1382, 1564, 1609, 2662
BmrFI	CCNGG	3 cut(s)	157, 1479, 1653
BmrI	ACTGGG	1 cut(s)	2041
BmtI	GCTAGC	1 cut(s)	2537
BmuI	ACTGGG	1 cut(s)	2041
BpiI	GAAGAC	2 cut(s)	624, 1047
BplI	GAGNNNNNCTC	2 cut(s)	264, 296
BpmI	CTGGAG	4 cut(s)	663, 1386, 1443, 2187
Bpu10I	CCTNAGC	1 cut(s)	173
BpuEI	CTTGAG	3 cut(s)	286, 644, 1888
BpuMI	CCSGG	2 cut(s)	157, 1653
BsaBI	GATNNNNATC	1 cut(s)	2058
BsaJI	CCNNGG	5 cut(s)	481, 1185, 2117, 2445, 2677
BsaWI	WCCGGW	2 cut(s)	144, 1487
BsaXI	ACNNNNNCTCC	2 cut(s)	93, 123
Bsc4I	CCNNNNNNNGG	7 cut(s)	192, 398, 1364, 1391, 1573, 2256, 2257
Bse118I	RCCGGY	1 cut(s)	144
Bse1I	ACTGG	5 cut(s)	1369, 1460, 1673, 2036, 2714
Bse3DI	GCAATG	4 cut(s)	1245, 2337, 2427, 2646
Bse8I	GATNNNNATC	1 cut(s)	2058
BseBI	CCWGG	1 cut(s)	1479
BseDI	CCNNGG	5 cut(s)	481, 1185, 2117, 2445, 2677
BseGI	GGATG	5 cut(s)	499, 1425, 1744, 2134, 2583
BseJI	GATNNNNATC	1 cut(s)	2058
BseLI	CCNNNNNNNGG	7 cut(s)	192, 398, 1364, 1391, 1573, 2256, 2257
BseMI	GCAATG	4 cut(s)	1245, 2337, 2427, 2646
BseMII	CTCAG	6 cut(s)	176, 187, 890, 1437, 1659, 2520
BseNI	ACTGG	5 cut(s)	1369, 1460, 1673, 2036, 2714
BseRI	GAGGAG	4 cut(s)	117, 647, 1464, 1820
BseXI	GCAGC	9 cut(s)	169, 484, 1801, 2002, 2232, 2347, 2377, 2527, 2530
BsgI	GTGCAG	1 cut(s)	734
Bsh1236I	CGCG	2 cut(s)	1892, 2124
BshFI	GGCC	6 cut(s)	160, 315, 1101, 1173, 2555, 2663
BshNI	GGYRCC	1 cut(s)	1576
BshTI	ACCGGT	1 cut(s)	144
BsiHKCI	CYCGRG	1 cut(s)	2218
BsiSI	CCGG	5 cut(s)	145, 157, 1488, 1653, 2309
BslFI	GGGAC	4 cut(s)	61, 224, 956, 1456
BslI	CCNNNNNNNGG	7 cut(s)	192, 398, 1364, 1391, 1573, 2256, 2257
BsmAI	GTCTC	5 cut(s)	255, 422, 906, 1074, 1243
BsmFI	GGGAC	4 cut(s)	61, 224, 956, 1456
BsmI	GAATGC	2 cut(s)	1868, 2247
BsnI	GGCC	6 cut(s)	160, 315, 1101, 1173, 2555, 2663
BsoBI	CYCGRG	1 cut(s)	2218
Bsp1286I	GDGCHC	1 cut(s)	2590
Bsp143I	GATC	9 cut(s)	275, 972, 1639, 1648, 1945, 2036, 2053, 2162, 2673
Bsp19I	CCATGG	1 cut(s)	1185
BspANI	GGCC	6 cut(s)	160, 315, 1101, 1173, 2555, 2663
BspCNI	CTCAG	6 cut(s)	175, 186, 891, 1438, 1658, 2521
BspFNI	CGCG	2 cut(s)	1892, 2124
BspHI	TCATGA	1 cut(s)	673
BspMI	ACCTGC	1 cut(s)	2509
BspOI	GCTAGC	1 cut(s)	2537
BspPI	GGATC	7 cut(s)	283, 1643, 2031, 2044, 2061, 2170, 2668
BspQI	GCTCTTC	1 cut(s)	2595
BspT107I	GGYRCC	1 cut(s)	1576
BsrDI	GCAATG	4 cut(s)	1245, 2337, 2427, 2646
BsrFI	RCCGGY	1 cut(s)	144
BsrI	ACTGG	5 cut(s)	1369, 1460, 1673, 2036, 2714
BssAI	RCCGGY	1 cut(s)	144
BssECI	CCNNGG	5 cut(s)	481, 1185, 2117, 2445, 2677
BssMI	GATC	9 cut(s)	275, 972, 1639, 1648, 1945, 2036, 2053, 2162, 2673
BssSI	CACGAG	1 cut(s)	1770
BssT1I	CCWWGG	4 cut(s)	1185, 2117, 2445, 2677
Bst2BI	CACGAG	1 cut(s)	1770
Bst2UI	CCWGG	1 cut(s)	1479
Bst4CI	ACNGT	9 cut(s)	62, 482, 861, 1068, 1128, 1678, 1797, 2372, 2696
Bst6I	CTCTTC	3 cut(s)	198, 587, 2595
BstAPI	GCANNNNNTGC	1 cut(s)	1931
BstC8I	GCNNGC	5 cut(s)	1812, 2047, 2535, 2637, 2661
BstDEI	CTNAG	8 cut(s)	162, 173, 451, 899, 1446, 1645, 1853, 2529
BstDSI	CCRYGG	2 cut(s)	481, 1185
BstENI	CCTNNNNNAGG	1 cut(s)	396
BstF5I	GGATG	5 cut(s)	499, 1425, 1744, 2134, 2583
BstFNI	CGCG	2 cut(s)	1892, 2124
BstHHI	GCGC	2 cut(s)	1355, 2124
BstKTI	GATC	9 cut(s)	278, 975, 1642, 1651, 1948, 2039, 2056, 2165, 2676
BstMAI	GTCTC	5 cut(s)	255, 422, 906, 1074, 1243
BstMBI	GATC	9 cut(s)	275, 972, 1639, 1648, 1945, 2036, 2053, 2162, 2673
BstNI	CCWGG	1 cut(s)	1479
BstNSI	RCATGY	1 cut(s)	934
BstSCI	CCNGG	3 cut(s)	155, 1477, 1651
BstSFI	CTRYAG	3 cut(s)	549, 924, 1045
BstUI	CGCG	2 cut(s)	1892, 2124
BstV1I	GCAGC	9 cut(s)	169, 484, 1801, 2002, 2232, 2347, 2377, 2527, 2530
BstV2I	GAAGAC	2 cut(s)	624, 1047
BstX2I	RGATCY	5 cut(s)	275, 1648, 2036, 2053, 2162
BstYI	RGATCY	5 cut(s)	275, 1648, 2036, 2053, 2162
BsuRI	GGCC	6 cut(s)	160, 315, 1101, 1173, 2555, 2663
BtgI	CCRYGG	2 cut(s)	481, 1185
BtgZI	GCGATG	2 cut(s)	979, 1773
BtrI	CACGTC	1 cut(s)	2273
BtsCI	GGATG	5 cut(s)	499, 1425, 1744, 2134, 2583
BtsI	GCAGTG	1 cut(s)	1722
BtsIMutI	CAGTG	4 cut(s)	1064, 1674, 1722, 2368
BveI	ACCTGC	1 cut(s)	2509
Cac8I	GCNNGC	5 cut(s)	1812, 2047, 2535, 2637, 2661
CciI	TCATGA	1 cut(s)	673
CfoI	GCGC	2 cut(s)	1355, 2124
Cfr10I	RCCGGY	1 cut(s)	144
Cfr13I	GGNCC	9 cut(s)	169, 211, 577, 1171, 1321, 1382, 1564, 1609, 2662
CseI	GACGC	1 cut(s)	1898
Csp6I	GTAC	5 cut(s)	837, 1463, 1527, 1577, 1666
CspAI	ACCGGT	1 cut(s)	144
CviQI	GTAC	5 cut(s)	837, 1463, 1527, 1577, 1666
DdeI	CTNAG	8 cut(s)	162, 173, 451, 899, 1446, 1645, 1853, 2529
DpnI	GATC	9 cut(s)	277, 974, 1641, 1650, 1947, 2038, 2055, 2164, 2675
DpnII	GATC	9 cut(s)	275, 972, 1639, 1648, 1945, 2036, 2053, 2162, 2673
DraI	TTTAAA	2 cut(s)	1554, 2617
DrdI	GACNNNNNNGTC	2 cut(s)	935, 1319
DseDI	GACNNNNNNGTC	2 cut(s)	935, 1319
EaeI	YGGCCR	1 cut(s)	1099
Eam1104I	CTCTTC	3 cut(s)	198, 587, 2595
EarI	CTCTTC	3 cut(s)	198, 587, 2595
EciI	GGCGGA	1 cut(s)	1622
Eco130I	CCWWGG	4 cut(s)	1185, 2117, 2445, 2677
Eco24I	GRGCYC	1 cut(s)	2590
Eco47I	GGWCC	7 cut(s)	169, 211, 577, 1321, 1382, 1564, 1609
Eco57I	CTGAAG	5 cut(s)	1074, 1157, 2376, 2415, 2445
Eco88I	CYCGRG	1 cut(s)	2218
EcoNI	CCTNNNNNAGG	1 cut(s)	396
EcoO109I	RGGNCCY	2 cut(s)	211, 1564
EcoRII	CCWGG	1 cut(s)	1477
EcoT14I	CCWWGG	4 cut(s)	1185, 2117, 2445, 2677
EcoT38I	GRGCYC	1 cut(s)	2590
ErhI	CCWWGG	4 cut(s)	1185, 2117, 2445, 2677
FalI	AAGNNNNNCTT	4 cut(s)	579, 611, 1995, 2027
FaqI	GGGAC	4 cut(s)	61, 224, 956, 1456
FauI	CCCGC	3 cut(s)	223, 801, 2392
FauNDI	CATATG	1 cut(s)	1819
FokI	GGATG	5 cut(s)	506, 1432, 1751, 2141, 2590
FriOI	GRGCYC	1 cut(s)	2590
FspBI	CTAG	8 cut(s)	398, 635, 749, 1002, 2237, 2252, 2435, 2534
GlaI	GCGC	2 cut(s)	1354, 2123
GsuI	CTGGAG	4 cut(s)	663, 1386, 1443, 2187
HaeIII	GGCC	6 cut(s)	160, 315, 1101, 1173, 2555, 2663
HapII	CCGG	5 cut(s)	145, 157, 1488, 1653, 2309
HgaI	GACGC	1 cut(s)	1898
HhaI	GCGC	2 cut(s)	1355, 2124
Hin6I	GCGC	2 cut(s)	1353, 2122
HinP1I	GCGC	2 cut(s)	1353, 2122
HincII	GTYRAC	4 cut(s)	754, 864, 905, 1888
HindII	GTYRAC	4 cut(s)	754, 864, 905, 1888
HindIII	AAGCTT	2 cut(s)	678, 2633
HinfI	GANTC	7 cut(s)	74, 139, 508, 901, 1375, 1840, 2650
HpaI	GTTAAC	2 cut(s)	754, 864
HpaII	CCGG	5 cut(s)	145, 157, 1488, 1653, 2309
HphI	GGTGA	6 cut(s)	62, 97, 134, 470, 1849, 2656
Hpy166II	GTNNAC	7 cut(s)	754, 864, 905, 1385, 1674, 1888, 2408
Hpy8I	GTNNAC	7 cut(s)	754, 864, 905, 1385, 1674, 1888, 2408
Hpy99I	CGWCG	1 cut(s)	2274
HpyCH4III	ACNGT	9 cut(s)	62, 482, 861, 1068, 1128, 1678, 1797, 2372, 2696
HpyCH4IV	ACGT	5 cut(s)	670, 1583, 1619, 2229, 2272
HpyF3I	CTNAG	8 cut(s)	162, 173, 451, 899, 1446, 1645, 1853, 2529
HpySE526I	ACGT	5 cut(s)	670, 1583, 1619, 2229, 2272
HspAI	GCGC	2 cut(s)	1353, 2122
KflI	GGGWCCC	1 cut(s)	211
KpnI	GGTACC	1 cut(s)	1580
KspAI	GTTAAC	2 cut(s)	754, 864
Kzo9I	GATC	9 cut(s)	275, 972, 1639, 1648, 1945, 2036, 2053, 2162, 2673
LguI	GCTCTTC	1 cut(s)	2595
LmnI	GCTCC	7 cut(s)	644, 1268, 1462, 1477, 1807, 1957, 2335
Lsp1109I	GCAGC	9 cut(s)	169, 484, 1801, 2002, 2232, 2347, 2377, 2527, 2530
MaeI	CTAG	8 cut(s)	398, 635, 749, 1002, 2237, 2252, 2435, 2534
MaeII	ACGT	5 cut(s)	670, 1583, 1619, 2229, 2272
MaeIII	GTNAC	2 cut(s)	50, 2372
MalI	GATC	9 cut(s)	277, 974, 1641, 1650, 1947, 2038, 2055, 2164, 2675
MboI	GATC	9 cut(s)	275, 972, 1639, 1648, 1945, 2036, 2053, 2162, 2673
MfeI	CAATTG	1 cut(s)	321
MflI	RGATCY	5 cut(s)	275, 1648, 2036, 2053, 2162
MhlI	GDGCHC	1 cut(s)	2590
MlsI	TGGCCA	1 cut(s)	1101
MluI	ACGCGT	1 cut(s)	1890
MluNI	TGGCCA	1 cut(s)	1101
MlyI	GAGTC	2 cut(s)	83, 910
MmeI	TCCRAC	3 cut(s)	147, 1566, 2293
Mox20I	TGGCCA	1 cut(s)	1101
MroXI	GAANNNNTTC	4 cut(s)	1084, 1260, 1341, 1593
MscI	TGGCCA	1 cut(s)	1101
MslI	CAYNNNNRTG	2 cut(s)	483, 689
Msp20I	TGGCCA	1 cut(s)	1101
MspA1I	CMGCKG	2 cut(s)	808, 2540
MspI	CCGG	5 cut(s)	145, 157, 1488, 1653, 2309
MspR9I	CCNGG	3 cut(s)	157, 1479, 1653
MunI	CAATTG	1 cut(s)	321
Mva1269I	GAATGC	2 cut(s)	1868, 2247
MvaI	CCWGG	1 cut(s)	1479
MvnI	CGCG	2 cut(s)	1892, 2124
NciI	CCSGG	2 cut(s)	157, 1653
NcoI	CCATGG	1 cut(s)	1185
NdeI	CATATG	1 cut(s)	1819
NdeII	GATC	9 cut(s)	275, 972, 1639, 1648, 1945, 2036, 2053, 2162, 2673
NheI	GCTAGC	1 cut(s)	2533
NmuCI	GTSAC	2 cut(s)	50, 2372
NspI	RCATGY	1 cut(s)	934
OliI	CACNNNNGTG	1 cut(s)	483
PagI	TCATGA	1 cut(s)	673
PciSI	GCTCTTC	1 cut(s)	2595
PctI	GAATGC	2 cut(s)	1868, 2247
PdmI	GAANNNNTTC	4 cut(s)	1084, 1260, 1341, 1593
PfeI	GAWTC	5 cut(s)	139, 508, 1375, 1840, 2650
PflMI	CCANNNNNTGG	2 cut(s)	1364, 1391
PfoI	TCCNGGA	1 cut(s)	1477
PinAI	ACCGGT	1 cut(s)	144
PleI	GAGTC	2 cut(s)	82, 909
PpsI	GAGTC	2 cut(s)	82, 909
PpuMI	RGGWCCY	2 cut(s)	211, 1564
PsiI	TTATAA	1 cut(s)	1092
Psp5II	RGGWCCY	2 cut(s)	211, 1564
Psp6I	CCWGG	1 cut(s)	1477
PspGI	CCWGG	1 cut(s)	1477
PspPI	GGNCC	9 cut(s)	169, 211, 577, 1171, 1321, 1382, 1564, 1609, 2662
PspPPI	RGGWCCY	2 cut(s)	211, 1564
PsrI	GAACNNNNNNTAC	2 cut(s)	1519, 1551
PsuI	RGATCY	5 cut(s)	275, 1648, 2036, 2053, 2162
RsaI	GTAC	5 cut(s)	838, 1464, 1528, 1578, 1667
RsaNI	GTAC	5 cut(s)	837, 1463, 1527, 1577, 1666
RseI	CAYNNNNRTG	2 cut(s)	483, 689
SapI	GCTCTTC	1 cut(s)	2595
Sau3AI	GATC	9 cut(s)	275, 972, 1639, 1648, 1945, 2036, 2053, 2162, 2673
Sau96I	GGNCC	9 cut(s)	169, 211, 577, 1171, 1321, 1382, 1564, 1609, 2662
ScaI	AGTACT	1 cut(s)	1667
SchI	GAGTC	2 cut(s)	83, 910
ScrFI	CCNGG	3 cut(s)	157, 1479, 1653
SduI	GDGCHC	1 cut(s)	2590
SfcI	CTRYAG	3 cut(s)	549, 924, 1045
SinI	GGWCC	7 cut(s)	169, 211, 577, 1321, 1382, 1564, 1609
SmiMI	CAYNNNNRTG	2 cut(s)	483, 689
SmlI	CTYRAG	3 cut(s)	301, 659, 1867
SmoI	CTYRAG	3 cut(s)	301, 659, 1867
SspI	AATATT	1 cut(s)	1096
SspMI	CTAG	8 cut(s)	398, 635, 749, 1002, 2237, 2252, 2435, 2534
StyD4I	CCNGG	3 cut(s)	155, 1477, 1651
StyI	CCWWGG	4 cut(s)	1185, 2117, 2445, 2677
TaaI	ACNGT	9 cut(s)	62, 482, 861, 1068, 1128, 1678, 1797, 2372, 2696
TaiI	ACGT	5 cut(s)	673, 1586, 1622, 2232, 2275
TaqI	TCGA	3 cut(s)	882, 2028, 2415
TaqII	GACCGA	1 cut(s)	965
TatI	WGTACW	1 cut(s)	1665
TauI	GCSGC	4 cut(s)	409, 2100, 2496, 2540
TfiI	GAWTC	5 cut(s)	139, 508, 1375, 1840, 2650
TscAI	CASTG	4 cut(s)	1071, 1681, 1722, 2375
TseFI	GTSAC	2 cut(s)	50, 2372
TseI	GCWGC	9 cut(s)	182, 472, 1814, 2015, 2245, 2360, 2390, 2540, 2543
Tsp45I	GTSAC	2 cut(s)	50, 2372
TspGWI	ACGGA	4 cut(s)	506, 1751, 2294, 2457
TspRI	CASTG	4 cut(s)	1071, 1681, 1722, 2375
Van91I	CCANNNNNTGG	2 cut(s)	1364, 1391
VpaK11BI	GGWCC	7 cut(s)	169, 211, 577, 1321, 1382, 1564, 1609
XagI	CCTNNNNNAGG	1 cut(s)	396
XapI	RAATTY	5 cut(s)	196, 649, 800, 2082, 2452
XbaI	TCTAGA	1 cut(s)	2434
XceI	RCATGY	1 cut(s)	934
XmnI	GAANNNNTTC	4 cut(s)	1084, 1260, 1341, 1593
XspI	CTAG	8 cut(s)	398, 635, 749, 1002, 2237, 2252, 2435, 2534
ZrmI	AGTACT	1 cut(s)	1667

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.