Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0004805

Physical Location & Seq

Genomic Coordinates Forward (+)

19961595 .. 19968823

Gene Structure 7229 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g15440.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGGCTCCGGCCACCGGAAGTTCGATTCACCGGTCGGGTTCCCGGCCTCAGTTGGACCTCAGCAAAGCTGCCATTCAAGGGAATTTTGAAGAGAGGGACCCTACAATTCTTCTGCCTAATCAATCTGATGACCTGTCTCACTTGGCTTTGGACATTGGAGGATCGCTCATCAAGTTGGTGTATTTCTCAAGACATGAAGGCCAATCAATTGATGATAAAAGGAAGAAAACTTTGAAGGAGAGATTGGGAATATCAAATGGTAGTAGGAGAAGCTATCCTATTCTTGGTGGGAGGCTTCATTTTGTGAAGTTTGAGACGAACAAGATTAACGAGTGCTTAGACTTCATCTATTCTAAGCAGCTTCACCGTGGTGGAATGGATGCTCGTATCTGGAATCCTGATGCCCCAACCAATGAAAGTTCTGTAATTAAGGCCACAGGTGGTGGGGCATACAAGTTTGCGGACCTCTTCAAAGAACGGCTTGGGGTTAGTCTTGACAAAGAAGACGAAATGAATTGTCTGGTGGCTGGAGCAAATTTTCTACTCAAGGCAATTCGTCATGAAGCTTTCACACACAAGGAGGGTCAGAAAGAGTTTGTGCAGATTGACCAGAATGAATTATTCCCGTATCTTCTAGTTAACATTGGGTCTGGTGTTAGTATGATTAAGGTTGATGGAGACGGAAAATTTCAGCGGATAAGTGGGACAAATGTTGGTGGTGGTACTTATTGGGGCTTGGGGAAACTGTTAACAAAGTGCAAAAGTTTTGACGAGTTGTTAGAGCTGAGTCAACGGGGAGATAATAGGACTATAGACATGCTTGTTGGGGACATTTATGGTGGTATGGACTACTCAAAGATCGGACTCTCTGCGTCAACCATCGCCTCTAGTTTTGGGAAGGCTATTTCAGAAAAAAAGGAGCTTGAAGACTACAGTCCTGAAGATATATCCTTGTCTCTCTTACGAATGATTTCTTATAATATTGGCCAGATAGCTTATCTAAATGCACTACGGTTTGGTCTGAAGCGAATATTTTTTGGAGGTTTTTTCATAAGGGGTCATGCTTATACCATGGACACTATCTCCTTTGCGGTTCAGTTCTGGTCAAAAGGAGAGGCACAAGCAATGTTCTTGAGACATGAAGGTTTTCTGGGAGCTTTAGGTGCATTTATGAGCTATGAAAAGCATGGTCTCGATGACTTGATGGTCCATCATTTAGTTGAAAGATTTCCTATGGGCGCACCATATACTGGAGGAAAGATTCATGGTCCACCACTTGGGGATTTGAACGAGAAGATTTCATGGATGGAAAAGTTTGTTCGCAAAGGGACTGAGATTACTGCTCCAGTACCAATGGCTCCACCTGGAACTACTGGACTTGGAGGCTTTGAAGTTCCTTCATCCAAAGGAGGTACTCTGCGTTCTGATGCAAGTGCTTTAAACGTTGGGGTTCTCCATCTGGTACCCACTTTGGAAGTCTTTCCATTATTGGCAGACCCAAAAACGTATGAACCCAACACTATCGATCTCTCAGATCACGGAGAGTTAGAGTACTGGTTCACTGTGCTGTCAGAGCATCTACCAGACCTTGTTGATAAGGCAGTGGCAAGTGAAGGTGGGACGGATGATGCTAAAAGAAGGGGTGATGCTTTTGCTCGTGCCTTTTCTGCTCACTTGGCACGGTTGATGGAGGAGCCTGCTGCATATGGAAAGTTAGGGTTGGCCAATCTATTGGAACTGAGAGAAGAATGCTTGAGGGAGTTCCAATTTGTTGATGCGTATAGAAGTATAAAACAAAGGGAAAATGAGGCATCACTTGCTGTTTTAGCTGATCTGCTTATGGAGCTCGATGGTATGAGTGAGGAAGCAAGACTGCTTACCCTTATTGAAGGTGTTCTTGCTGCGAACATTTTCGACTGGGGATCCCGTGCTTGCGTGGATCTCTATCACAAAGGAACGATTATTGAAATTTATAGAATGAGCCGCAATAAGATGCAAAGACCTTGGCGCGTGGACGACTTTGATGTTTTCAAAGAGAGAATGTTAGGATCTGGAGATAAAAAACCTCGTCCACATAAAAGAGCTTTGCTATTTGTGGATAACTCGGGTGCTGACGTTATTCTAGGAATGCTACCCTTAGCACGGGAACTTCTCCGACGGGGAACAGAAGTTGTTTTGGTTGCCAACTCCCTTCCAGCTCTAAATGATGTGACTGCAATGGAGCTTCCTGAAATTGTTGCCGAAGCTGCCAAGCATTGTGACATTCTTCGCAGAGCTGCTGAAGCGGGAGGCTTGCTTGTGGATGCAATGGTTAGCACTCTAGATGGCTCTAAAGGAAATTCATCTTCGGTTCCCTTGATGGTTGTTGAGAATGGTTGCGGCAGTCCTTGCATAGACTTACGGCAAGTTAGCTCTGAGCTAGCTGCTGCTGCAAAAGAGGCCGATTTGGTTATCTTGGAAGGCATGGGCAGAGCTCTTCATACCAACTTTAATGCGAAGTTTAAATGCGACGCTTTAAAGCTTGCAATGGTGAAGAACCAAAGGTTGGCCCAAAAACTGATCAAAGGTGACATATACGACTGCGTTTGCAGATACGAACCAGTAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004594 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0044237 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00770 ko01100 map00770 map01100

Pfam Domains

Protein Families

DUF89 Fumble

Protein Analysis

870

Amino Acids

95.69

Weight (kDa)

6.02

Isoelectric Point (pI)

35.53

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004805)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32180 AT4G32180 AT4G32180
fragaria_vesca	FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350 FvH4_5g05350
malus_domestica	MD06G1173300.v1.1 MD14G1179800.v1.1
prunus_persica	Prupe.5G172800_v2.0.a1 Prupe.5G172800_v2.0.a1
pyrus_communis	pycom06g15440 pycom14g14960
rosa_chinensis	RchiOBHm_Chr7g0193481
rosa_laevigata	RLG00000004267
rosa_multiflora	Rmu_sc0006629.1_g000003 Rmu_sc0012783.1_g000004 Rmu_sc0031135.1_g000001
rosa_roxburghii	Rroxscaffold_3G00262060
rosa_rugosa	Rorug07G0009700 Rorug07G0009700
rosa_samantha	Rh7AG136400 Rh7BG136800 Rh7CG140200 Rh7DG138000
rosa_wichuraiana	Rw7G011630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	978
AasI	GACNNNNNNGTC	1 cut(s)	1205
Acc65I	GGTACC	1 cut(s)	1462
AccB1I	GGYRCC	1 cut(s)	1462
AccB7I	CCANNNNNTGG	3 cut(s)	1250, 1277, 2545
AccII	CGCG	1 cut(s)	2010
AciI	CCGC	6 cut(s)	461, 694, 1091, 1984, 2285, 2379
AclI	AACGTT	1 cut(s)	1443
AclWI	GGATC	5 cut(s)	169, 1917, 1930, 1947, 2056
AcoI	YGGCCR	3 cut(s)	9, 985, 1722
AcsI	RAATTY	5 cut(s)	82, 535, 686, 1968, 2338
AcuI	CTGAAG	3 cut(s)	960, 1043, 2301
AfaI	GTAC	5 cut(s)	724, 1350, 1414, 1464, 1553
AfiI	CCNNNNNNNGG	7 cut(s)	14, 78, 284, 1250, 1277, 2142, 2545
AgeI	ACCGGT	1 cut(s)	30
AjnI	CCWGG	1 cut(s)	1363
AjuI	GAANNNNNNNTTGG	4 cut(s)	227, 259, 2528, 2560
AleI	CACNNNNGTG	1 cut(s)	369
Alw21I	GWGCWC	2 cut(s)	1848, 2476
Alw26I	GTCTC	6 cut(s)	141, 308, 672, 960, 1129, 1196
AlwI	GGATC	5 cut(s)	169, 1917, 1930, 1947, 2056
Ama87I	CYCGRG	1 cut(s)	2104
AoxI	GGCC	8 cut(s)	9, 44, 199, 432, 985, 1722, 2439, 2547
ApeKI	GCWGC	9 cut(s)	68, 358, 1700, 1901, 2246, 2276, 2423, 2426, 2429
ApoI	RAATTY	5 cut(s)	82, 535, 686, 1968, 2338
AsiGI	ACCGGT	1 cut(s)	30
Asp700I	GAANNNNTTC	6 cut(s)	970, 1146, 1227, 1479, 1893, 1910
Asp718I	GGTACC	1 cut(s)	1462
AspLEI	GCGC	2 cut(s)	1241, 2010
AspS9I	GGNCC	6 cut(s)	55, 97, 463, 1207, 1268, 2548
AsuC2I	CCSGG	1 cut(s)	43
AsuHPI	GGTGA	5 cut(s)	20, 356, 1655, 2542, 2579
AsuNHI	GCTAGC	1 cut(s)	2419
AvaI	CYCGRG	1 cut(s)	2104
AvaII	GGWCC	5 cut(s)	55, 97, 463, 1207, 1268
BalI	TGGCCA	2 cut(s)	987, 1724
BamHI	GGATCC	1 cut(s)	1922
BanI	GGYRCC	1 cut(s)	1462
BanII	GRGCYC	2 cut(s)	1848, 2476
BauI	CACGAG	1 cut(s)	1656
BbsI	GAAGAC	2 cut(s)	510, 933
Bbv12I	GWGCWC	2 cut(s)	1848, 2476
BbvCI	CCTCAGC	1 cut(s)	59
BbvI	GCAGC	9 cut(s)	55, 370, 1687, 1888, 2233, 2263, 2410, 2413, 2416
BceAI	ACGGC	2 cut(s)	494, 2417
BciT130I	CCWGG	1 cut(s)	1365
BclI	TGATCA	1 cut(s)	2559
BcnI	CCSGG	1 cut(s)	43
BcoDI	GTCTC	6 cut(s)	141, 308, 672, 960, 1129, 1196
BfaI	CTAG	5 cut(s)	635, 888, 2123, 2321, 2420
BfmI	CTRYAG	2 cut(s)	810, 931
BmcAI	AGTACT	1 cut(s)	1553
Bme1390I	CCNGG	2 cut(s)	43, 1365
Bme18I	GGWCC	5 cut(s)	55, 97, 463, 1207, 1268
BmeT110I	CYCGRG	1 cut(s)	2104
BmgT120I	GGNCC	6 cut(s)	55, 97, 463, 1207, 1268, 2548
BmiI	GGNNCC	9 cut(s)	6, 40, 98, 99, 1359, 1464, 1695, 1924, 2352
BmrFI	CCNGG	2 cut(s)	43, 1365
BmrI	ACTGGG	1 cut(s)	1927
BmtI	GCTAGC	1 cut(s)	2423
BmuI	ACTGGG	1 cut(s)	1927
BoxI	GACNNNNGTC	1 cut(s)	933
BpiI	GAAGAC	2 cut(s)	510, 933
BplI	GAGNNNNNCTC	2 cut(s)	150, 182
BpmI	CTGGAG	4 cut(s)	549, 1272, 1329, 2073
Bpu10I	CCTNAGC	2 cut(s)	59, 2137
BpuEI	CTTGAG	4 cut(s)	172, 530, 1153, 1774
BpuMI	CCSGG	1 cut(s)	43
Bsa29I	ATCGAT	1 cut(s)	1524
BsaBI	GATNNNNATC	1 cut(s)	1944
BsaI	GGTCTC	1 cut(s)	1196
BsaJI	CCNNGG	3 cut(s)	367, 1071, 2003
BsaWI	WCCGGW	2 cut(s)	14, 30
BsaXI	ACNNNNNCTCC	2 cut(s)	1067, 1097
Bsc4I	CCNNNNNNNGG	7 cut(s)	14, 78, 284, 1250, 1277, 2142, 2545
Bse118I	RCCGGY	1 cut(s)	30
Bse1I	ACTGG	6 cut(s)	1255, 1346, 1378, 1559, 1922, 2600
Bse3DI	GCAATG	4 cut(s)	1131, 2223, 2313, 2532
Bse8I	GATNNNNATC	1 cut(s)	1944
BseBI	CCWGG	1 cut(s)	1365
BseCI	ATCGAT	1 cut(s)	1524
BseDI	CCNNGG	3 cut(s)	367, 1071, 2003
BseGI	GGATG	5 cut(s)	385, 1311, 1400, 1630, 2308
BseJI	GATNNNNATC	1 cut(s)	1944
BseLI	CCNNNNNNNGG	7 cut(s)	14, 78, 284, 1250, 1277, 2142, 2545
BseMI	GCAATG	4 cut(s)	1131, 2223, 2313, 2532
BseMII	CTCAG	7 cut(s)	62, 73, 776, 1323, 1545, 1730, 2406
BseNI	ACTGG	6 cut(s)	1255, 1346, 1378, 1559, 1922, 2600
BseRI	GAGGAG	1 cut(s)	1706
BseXI	GCAGC	9 cut(s)	55, 370, 1687, 1888, 2233, 2263, 2410, 2413, 2416
BsgI	GTGCAG	1 cut(s)	620
Bsh1236I	CGCG	1 cut(s)	2010
Bsh1285I	CGRYCG	1 cut(s)	35
BshFI	GGCC	8 cut(s)	11, 46, 201, 434, 987, 1724, 2441, 2549
BshNI	GGYRCC	1 cut(s)	1462
BshTI	ACCGGT	1 cut(s)	30
BshVI	ATCGAT	1 cut(s)	1524
BsiEI	CGRYCG	1 cut(s)	35
BsiHKAI	GWGCWC	2 cut(s)	1848, 2476
BsiHKCI	CYCGRG	1 cut(s)	2104
BsiSI	CCGG	4 cut(s)	8, 15, 31, 43
BslFI	GGGAC	5 cut(s)	110, 718, 842, 1342, 1633
BslI	CCNNNNNNNGG	7 cut(s)	14, 78, 284, 1250, 1277, 2142, 2545
BsmAI	GTCTC	6 cut(s)	141, 308, 672, 960, 1129, 1196
BsmBI	CGTCTC	2 cut(s)	308, 672
BsmFI	GGGAC	5 cut(s)	110, 718, 842, 1342, 1633
BsmI	GAATGC	2 cut(s)	1754, 2133
BsnI	GGCC	8 cut(s)	11, 46, 201, 434, 987, 1724, 2441, 2549
Bso31I	GGTCTC	1 cut(s)	1196
BsoBI	CYCGRG	1 cut(s)	2104
Bsp1286I	GDGCHC	2 cut(s)	1848, 2476
Bsp143I	GATC	9 cut(s)	161, 858, 1525, 1534, 1831, 1922, 1939, 2048, 2559
Bsp19I	CCATGG	1 cut(s)	1071
BspACI	CCGC	6 cut(s)	461, 694, 1091, 1984, 2285, 2379
BspANI	GGCC	8 cut(s)	11, 46, 201, 434, 987, 1724, 2441, 2549
BspCNI	CTCAG	7 cut(s)	61, 72, 777, 1324, 1544, 1731, 2407
BspDI	ATCGAT	1 cut(s)	1524
BspFNI	CGCG	1 cut(s)	2010
BspHI	TCATGA	1 cut(s)	559
BspLI	GGNNCC	9 cut(s)	6, 40, 98, 99, 1359, 1464, 1695, 1924, 2352
BspOI	GCTAGC	1 cut(s)	2423
BspPI	GGATC	5 cut(s)	169, 1917, 1930, 1947, 2056
BspQI	GCTCTTC	1 cut(s)	2481
BspT107I	GGYRCC	1 cut(s)	1462
BspTNI	GGTCTC	1 cut(s)	1196
BsrDI	GCAATG	4 cut(s)	1131, 2223, 2313, 2532
BsrFI	RCCGGY	1 cut(s)	30
BsrI	ACTGG	6 cut(s)	1255, 1346, 1378, 1559, 1922, 2600
BssAI	RCCGGY	1 cut(s)	30
BssECI	CCNNGG	3 cut(s)	367, 1071, 2003
BssMI	GATC	9 cut(s)	161, 858, 1525, 1534, 1831, 1922, 1939, 2048, 2559
BssSI	CACGAG	1 cut(s)	1656
BssT1I	CCWWGG	2 cut(s)	1071, 2003
Bst2BI	CACGAG	1 cut(s)	1656
Bst2UI	CCWGG	1 cut(s)	1365
Bst4CI	ACNGT	6 cut(s)	368, 747, 935, 1014, 1564, 1683
Bst6I	CTCTTC	3 cut(s)	84, 473, 2481
BstAPI	GCANNNNNTGC	2 cut(s)	1817, 2388
BstC8I	GCNNGC	5 cut(s)	1698, 1933, 2294, 2421, 2523
BstDSI	CCRYGG	2 cut(s)	367, 1071
BstF5I	GGATG	5 cut(s)	385, 1311, 1400, 1630, 2308
BstFNI	CGCG	1 cut(s)	2010
BstHHI	GCGC	2 cut(s)	1241, 2010
BstKTI	GATC	9 cut(s)	164, 861, 1528, 1537, 1834, 1925, 1942, 2051, 2562
BstMAI	GTCTC	6 cut(s)	141, 308, 672, 960, 1129, 1196
BstMBI	GATC	9 cut(s)	161, 858, 1525, 1534, 1831, 1922, 1939, 2048, 2559
BstMCI	CGRYCG	1 cut(s)	35
BstNI	CCWGG	1 cut(s)	1365
BstNSI	RCATGY	1 cut(s)	820
BstPAI	GACNNNNGTC	1 cut(s)	933
BstSCI	CCNGG	2 cut(s)	41, 1363
BstSFI	CTRYAG	2 cut(s)	810, 931
BstUI	CGCG	1 cut(s)	2010
BstV1I	GCAGC	9 cut(s)	55, 370, 1687, 1888, 2233, 2263, 2410, 2413, 2416
BstV2I	GAAGAC	2 cut(s)	510, 933
BstX2I	RGATCY	3 cut(s)	1922, 1939, 2048
BstXI	CCANNNNNNTGG	1 cut(s)	1732
BstYI	RGATCY	3 cut(s)	1922, 1939, 2048
Bsu15I	ATCGAT	1 cut(s)	1524
BsuRI	GGCC	8 cut(s)	11, 46, 201, 434, 987, 1724, 2441, 2549
BsuTUI	ATCGAT	1 cut(s)	1524
BtgI	CCRYGG	2 cut(s)	367, 1071
BtgZI	GCGATG	1 cut(s)	865
BtsCI	GGATG	5 cut(s)	385, 1311, 1400, 1630, 2308
BtsI	GCAGTG	1 cut(s)	1608
BtsIMutI	CAGTG	2 cut(s)	1560, 1608
Cac8I	GCNNGC	5 cut(s)	1698, 1933, 2294, 2421, 2523
CciI	TCATGA	1 cut(s)	559
CfoI	GCGC	2 cut(s)	1241, 2010
Cfr10I	RCCGGY	1 cut(s)	30
Cfr13I	GGNCC	6 cut(s)	55, 97, 463, 1207, 1268, 2548
ClaI	ATCGAT	1 cut(s)	1524
CseI	GACGC	2 cut(s)	861, 2519
Csp6I	GTAC	5 cut(s)	723, 1349, 1413, 1463, 1552
CspAI	ACCGGT	1 cut(s)	30
CviQI	GTAC	5 cut(s)	723, 1349, 1413, 1463, 1552
DpnI	GATC	9 cut(s)	163, 860, 1527, 1536, 1833, 1924, 1941, 2050, 2561
DpnII	GATC	9 cut(s)	161, 858, 1525, 1534, 1831, 1922, 1939, 2048, 2559
DraI	TTTAAA	3 cut(s)	1440, 2503, 2517
DrdI	GACNNNNNNGTC	1 cut(s)	1205
DseDI	GACNNNNNNGTC	1 cut(s)	1205
EaeI	YGGCCR	3 cut(s)	9, 985, 1722
Eam1104I	CTCTTC	3 cut(s)	84, 473, 2481
EarI	CTCTTC	3 cut(s)	84, 473, 2481
Ecl136II	GAGCTC	2 cut(s)	1846, 2474
Eco130I	CCWWGG	2 cut(s)	1071, 2003
Eco24I	GRGCYC	2 cut(s)	1848, 2476
Eco31I	GGTCTC	1 cut(s)	1196
Eco47I	GGWCC	5 cut(s)	55, 97, 463, 1207, 1268
Eco53kI	GAGCTC	2 cut(s)	1846, 2474
Eco57I	CTGAAG	3 cut(s)	960, 1043, 2301
Eco88I	CYCGRG	1 cut(s)	2104
EcoICRI	GAGCTC	2 cut(s)	1846, 2474
EcoO109I	RGGNCCY	1 cut(s)	97
EcoRII	CCWGG	1 cut(s)	1363
EcoT14I	CCWWGG	2 cut(s)	1071, 2003
EcoT38I	GRGCYC	2 cut(s)	1848, 2476
ErhI	CCWWGG	2 cut(s)	1071, 2003
Esp3I	CGTCTC	2 cut(s)	308, 672
FalI	AAGNNNNNCTT	4 cut(s)	465, 497, 1881, 1913
FaqI	GGGAC	5 cut(s)	110, 718, 842, 1342, 1633
FauI	CCCGC	1 cut(s)	2278
FauNDI	CATATG	1 cut(s)	1705
FbaI	TGATCA	1 cut(s)	2559
FokI	GGATG	5 cut(s)	392, 1318, 1387, 1637, 2315
FriOI	GRGCYC	2 cut(s)	1848, 2476
FspBI	CTAG	5 cut(s)	635, 888, 2123, 2321, 2420
GlaI	GCGC	2 cut(s)	1240, 2009
GsuI	CTGGAG	4 cut(s)	549, 1272, 1329, 2073
HaeIII	GGCC	8 cut(s)	11, 46, 201, 434, 987, 1724, 2441, 2549
HapII	CCGG	4 cut(s)	8, 15, 31, 43
HgaI	GACGC	2 cut(s)	861, 2519
HhaI	GCGC	2 cut(s)	1241, 2010
Hin6I	GCGC	2 cut(s)	1239, 2008
HinP1I	GCGC	2 cut(s)	1239, 2008
HincII	GTYRAC	4 cut(s)	640, 750, 791, 876
HindII	GTYRAC	4 cut(s)	640, 750, 791, 876
HindIII	AAGCTT	2 cut(s)	564, 2519
HinfI	GANTC	5 cut(s)	25, 394, 787, 864, 1261
HpaI	GTTAAC	2 cut(s)	640, 750
HpaII	CCGG	4 cut(s)	8, 15, 31, 43
HphI	GGTGA	5 cut(s)	20, 356, 1655, 2542, 2579
Hpy166II	GTNNAC	8 cut(s)	640, 750, 791, 876, 1271, 1560, 2014, 2072
Hpy8I	GTNNAC	8 cut(s)	640, 750, 791, 876, 1271, 1560, 2014, 2072
Hpy99I	CGWCG	2 cut(s)	2160, 2513
HpyCH4III	ACNGT	6 cut(s)	368, 747, 935, 1014, 1564, 1683
HpyCH4IV	ACGT	3 cut(s)	1443, 1505, 2115
HpySE526I	ACGT	3 cut(s)	1443, 1505, 2115
HspAI	GCGC	2 cut(s)	1239, 2008
KflI	GGGWCCC	1 cut(s)	97
KpnI	GGTACC	1 cut(s)	1466
Ksp22I	TGATCA	1 cut(s)	2559
KspAI	GTTAAC	2 cut(s)	640, 750
Kzo9I	GATC	9 cut(s)	161, 858, 1525, 1534, 1831, 1922, 1939, 2048, 2559
LguI	GCTCTTC	1 cut(s)	2481
LmnI	GCTCC	9 cut(s)	10, 530, 919, 1154, 1348, 1363, 1693, 1843, 2221
Lsp1109I	GCAGC	9 cut(s)	55, 370, 1687, 1888, 2233, 2263, 2410, 2413, 2416
MaeI	CTAG	5 cut(s)	635, 888, 2123, 2321, 2420
MaeII	ACGT	3 cut(s)	1443, 1505, 2115
MaeIII	GTNAC	3 cut(s)	2209, 2258, 2567
MalI	GATC	9 cut(s)	163, 860, 1527, 1536, 1833, 1924, 1941, 2050, 2561
MboI	GATC	9 cut(s)	161, 858, 1525, 1534, 1831, 1922, 1939, 2048, 2559
MfeI	CAATTG	1 cut(s)	207
MflI	RGATCY	3 cut(s)	1922, 1939, 2048
MhlI	GDGCHC	2 cut(s)	1848, 2476
MlsI	TGGCCA	2 cut(s)	987, 1724
MluNI	TGGCCA	2 cut(s)	987, 1724
MlyI	GAGTC	2 cut(s)	796, 858
MmeI	TCCRAC	2 cut(s)	33, 2179
Mox20I	TGGCCA	2 cut(s)	987, 1724
MroXI	GAANNNNTTC	6 cut(s)	970, 1146, 1227, 1479, 1893, 1910
MscI	TGGCCA	2 cut(s)	987, 1724
MslI	CAYNNNNRTG	1 cut(s)	369
Msp20I	TGGCCA	2 cut(s)	987, 1724
MspA1I	CMGCKG	1 cut(s)	694
MspI	CCGG	4 cut(s)	8, 15, 31, 43
MspR9I	CCNGG	2 cut(s)	43, 1365
MunI	CAATTG	1 cut(s)	207
Mva1269I	GAATGC	2 cut(s)	1754, 2133
MvaI	CCWGG	1 cut(s)	1365
MvnI	CGCG	1 cut(s)	2010
NciI	CCSGG	1 cut(s)	43
NcoI	CCATGG	1 cut(s)	1071
NdeI	CATATG	1 cut(s)	1705
NdeII	GATC	9 cut(s)	161, 858, 1525, 1534, 1831, 1922, 1939, 2048, 2559
NheI	GCTAGC	1 cut(s)	2419
NlaIV	GGNNCC	9 cut(s)	6, 40, 98, 99, 1359, 1464, 1695, 1924, 2352
NmuCI	GTSAC	3 cut(s)	2209, 2258, 2567
NspI	RCATGY	1 cut(s)	820
OliI	CACNNNNGTG	1 cut(s)	369
PagI	TCATGA	1 cut(s)	559
PciSI	GCTCTTC	1 cut(s)	2481
PctI	GAATGC	2 cut(s)	1754, 2133
PdmI	GAANNNNTTC	6 cut(s)	970, 1146, 1227, 1479, 1893, 1910
PfeI	GAWTC	3 cut(s)	25, 394, 1261
PflMI	CCANNNNNTGG	3 cut(s)	1250, 1277, 2545
PinAI	ACCGGT	1 cut(s)	30
PleI	GAGTC	2 cut(s)	795, 858
PpsI	GAGTC	2 cut(s)	795, 858
PpuMI	RGGWCCY	1 cut(s)	97
PshAI	GACNNNNGTC	1 cut(s)	933
PsiI	TTATAA	1 cut(s)	978
Psp124BI	GAGCTC	2 cut(s)	1848, 2476
Psp1406I	AACGTT	1 cut(s)	1443
Psp5II	RGGWCCY	1 cut(s)	97
Psp6I	CCWGG	1 cut(s)	1363
PspGI	CCWGG	1 cut(s)	1363
PspN4I	GGNNCC	9 cut(s)	6, 40, 98, 99, 1359, 1464, 1695, 1924, 2352
PspPI	GGNCC	6 cut(s)	55, 97, 463, 1207, 1268, 2548
PspPPI	RGGWCCY	1 cut(s)	97
PsrI	GAACNNNNNNTAC	2 cut(s)	1405, 1437
PsuI	RGATCY	3 cut(s)	1922, 1939, 2048
RsaI	GTAC	5 cut(s)	724, 1350, 1414, 1464, 1553
RsaNI	GTAC	5 cut(s)	723, 1349, 1413, 1463, 1552
RseI	CAYNNNNRTG	1 cut(s)	369
SacI	GAGCTC	2 cut(s)	1848, 2476
SapI	GCTCTTC	1 cut(s)	2481
Sau3AI	GATC	9 cut(s)	161, 858, 1525, 1534, 1831, 1922, 1939, 2048, 2559
Sau96I	GGNCC	6 cut(s)	55, 97, 463, 1207, 1268, 2548
ScaI	AGTACT	1 cut(s)	1553
SchI	GAGTC	2 cut(s)	796, 858
ScrFI	CCNGG	2 cut(s)	43, 1365
SduI	GDGCHC	2 cut(s)	1848, 2476
SfcI	CTRYAG	2 cut(s)	810, 931
SinI	GGWCC	5 cut(s)	55, 97, 463, 1207, 1268
SmiMI	CAYNNNNRTG	1 cut(s)	369
SmlI	CTYRAG	4 cut(s)	187, 545, 1132, 1753
SmoI	CTYRAG	4 cut(s)	187, 545, 1132, 1753
SsiI	CCGC	6 cut(s)	461, 694, 1091, 1984, 2285, 2379
SspI	AATATT	2 cut(s)	982, 1032
SspMI	CTAG	5 cut(s)	635, 888, 2123, 2321, 2420
SstI	GAGCTC	2 cut(s)	1848, 2476
StyD4I	CCNGG	2 cut(s)	41, 1363
StyI	CCWWGG	2 cut(s)	1071, 2003
TaaI	ACNGT	6 cut(s)	368, 747, 935, 1014, 1564, 1683
TaiI	ACGT	3 cut(s)	1446, 1508, 2118
TaqI	TCGA	5 cut(s)	23, 1194, 1524, 1848, 1914
TatI	WGTACW	1 cut(s)	1551
TauI	GCSGC	2 cut(s)	1986, 2382
TfiI	GAWTC	3 cut(s)	25, 394, 1261
TscAI	CASTG	2 cut(s)	1567, 1608
TseFI	GTSAC	3 cut(s)	2209, 2258, 2567
TseI	GCWGC	9 cut(s)	68, 358, 1700, 1901, 2246, 2276, 2423, 2426, 2429
Tsp45I	GTSAC	3 cut(s)	2209, 2258, 2567
TspGWI	ACGGA	3 cut(s)	696, 1554, 1637
TspRI	CASTG	2 cut(s)	1567, 1608
Van91I	CCANNNNNTGG	3 cut(s)	1250, 1277, 2545
VpaK11BI	GGWCC	5 cut(s)	55, 97, 463, 1207, 1268
XapI	RAATTY	5 cut(s)	82, 535, 686, 1968, 2338
XbaI	TCTAGA	1 cut(s)	2320
XceI	RCATGY	1 cut(s)	820
XmnI	GAANNNNTTC	6 cut(s)	970, 1146, 1227, 1479, 1893, 1910
XspI	CTAG	5 cut(s)	635, 888, 2123, 2321, 2420
ZrmI	AGTACT	1 cut(s)	1553

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.