MD14G1206000.v1.1

Protein WEAK CHLOROPLAST MOVEMENT UNDER BLUE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015628
Physical Location & Seq
Forward (+)
29327106 .. 29329754
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1206000.v1.1.491

Sequence Viewer

Length: 1896 bp
ATGGGGGAGATTGATACAAAACCAATTGAACCAGTCCAGGCTGCATTGTGCCTGTTTGAAGAGAAGAACGATCACCGGAAAACCCGGCTTTCCGGCACAGATGTAAGTGATCGTAAGGATAGTGAGAAAGAGAGAGAGCTTGAAGGTGTGTTGAAGGATTTGGCCAATTACAAGGTGCAGCTGGAAGCAAAAGATGCTTCCTACATGCAAGCCCTTCTCAAGCTGCAACACCACGAGTACACAGCGGAGGAACTCTCTGCCCTCCTTGAAAACATCGAGTTTGAGAGAGATAAGTGCGTCGAGGAATGTAAAGAGGCTCGGGCGCAGATATACCAACTCGAATCCAAGGTAAAGGAAATGGGGGATCAGCTGTCTGAGACTCAGAAGGTGAGAGAGCAACTCATGCATGTTTTTAGTGAGTTGAAGGCTACACAATCAGAGCTGCTTGGCATGGAAACAAAACTAGCGGACGCTAGAGATTCGGAGCTAAAAGCTCTAACGCAAGCGGAGCTAATGGAAACAGCTTTCGTGATGGAAAAGGAGAGGGTGGAAGAGCTTCTTAAACATGTCTCCGAGCTAAGTGAAGCCGTGGTTAAGTCAAAGGTTGCTGCTAGTGAAACAGATAAAGAGAAGTTGGCGATATTATCTGCGAAGGATACTGAGATTGAGTTAGCTACACAGGCTGCTCTTCAAGAGCAGGAGCAGCTCGAATATTCTAAGAAACAAACGGTGAAGATGGAGGAGTTGGAGGATCAGCTCCTCGCCAAGTCTTCGTTCATTGAGATGCTTCAGCTGGAGATTAAACAGGTGAATGAAAACCTTAGTTTCTCTAAGATGGCGTCTTCTGATGCGATGAATGACTTGAAGCAGCTGGAAAAAGAGTTAGAAGAGAAGGAAAGAAAGATCTCGGATCAGGAAGGTTTCATCGAGGGGCTAGAAATGGAACTGAATCAGTTAAAATTGGAGCTTAGTGATGCAAATCAAGTGGCCAACAGCCTGAAGCACGACATTGAAGTTCTTACTGAGGATTTACAGAAAGCAAAAATGCAGCTTGATGAGATGGCGGAAAGGGAAAACAATGCGCAGGTTGAGATAGCAATGCTGGAATCTGAGCTTCATAGAGGGAGGTCAAAAATTGCAGCAGCAGAGGCAGCTGAAGAAAGAACCGAAAATGTTAAATCGGGGCTGTACCTTGCGATTCATCAACTAGTAATAGAAGCCGAGACTGCCAAGATGGAAAACAGAATGTTAAAGCAAGGAGCAGATAGCGCAGACAAAGAAACTGACGAACAATCTCCCCTTTTTGATTCTAATGAAGATGCTGAGGTTTCCAAAACTGGTGAACTTAAGGACGAAGCTGAAGAGCGAAAGGAAGAGAACGATTCCTATGTAACAATTTCATTGAAGGAATACAAGTACTTGGTTAAGAAGGCTGACCAGGCTGATCAAATTCCTTTGTCGTTGGTGGAAGCCTTTAGTCGTCAGTTGAGCTTCAGCACCGCAGGAGATAAGTCTGAGTTGGAACATTTGAAGAAGGAGTTGGAAGCTGCAATGGAAAAAGTTGCGCAATTCAGGAACCGGGCTGAGCAGGCTACTACTAGGGCCGATCTTGCCGAGAGAGCGAAAGAAAGACTAGAGGACCGGATAAGGATTTGGCGAAAGGTGAAGCAAAAGAGAAGAGCTGCTCTTGCTGCGCTTAGGGAGGTGTCTCTTCCTAAACAATTTAGCCCTCCCGCATATGAATCTCCAGATGAAATTAAGCCTCCTGCATATGAATCTCCAGATGAAATTAAGCCTCCTGCATATGAATCTCCAGATGAACTTATGCATAATGCATCACAAGAAACACAAAAACCATATCCGCAATTGTGTGACGTACTTAAAATGAAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WEMBL

Protein Analysis

632

Amino Acids

72.07

Weight (kDa)

4.8

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 3 - 427 4.4e-15 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14430
malus_domestica MD06G1198500.v1.1 MD14G1206000.v1.1
prunus_persica Prupe.5G199300_v2.0.a1 Prupe.5G199300_v2.0.a1
pyrus_communis pycom14g17040
rosa_chinensis RchiOBHm_Chr7g0182231
rosa_laevigata RLG00000005138
rosa_multiflora Rmu_sc0000003.1_g000012
rosa_roxburghii Rroxscaffold_3G00271400
rosa_rugosa Rorug06G0446300
rosa_samantha Rh7AG049300 Rh7BG049400 Rh7CG050700 Rh7DG048600
rosa_wichuraiana Rw7G004040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1083, 1566
Acc36I ACCTGC 1 cut(s) 1075
AciI CCGC 7 cut(s) 245, 467, 506, 1064, 1500, 1734, 1862
AclWI GGATC 3 cut(s) 372, 759, 918
AcoI YGGCCR 2 cut(s) 162, 987
AcsI RAATTY 2 cut(s) 1449, 1889
AcuI CTGAAG 5 cut(s) 773, 1019, 1176, 1380, 1477
AcyI GRCGYC 1 cut(s) 839
AfaI GTAC 4 cut(s) 239, 1190, 1418, 1878
AflII CTTAAG 1 cut(s) 1346
AflIII ACRYGT 1 cut(s) 565
AhlI ACTAGT 1 cut(s) 1207
AjnI CCWGG 2 cut(s) 36, 1437
AjuI GAANNNNNNNTTGG 4 cut(s) 758, 790, 1523, 1555
Alw26I GTCTC 4 cut(s) 371, 574, 1217, 1713
AlwI GGATC 3 cut(s) 372, 759, 918
Ama87I CYCGRG 1 cut(s) 318
AoxI GGCC 3 cut(s) 162, 987, 1602
ApoI RAATTY 2 cut(s) 1449, 1889
Asp700I GAANNNNTTC 1 cut(s) 555
AspLEI GCGC 5 cut(s) 325, 1084, 1271, 1567, 1696
AspS9I GGNCC 2 cut(s) 1602, 1639
AsuC2I CCSGG 2 cut(s) 85, 1580
AsuHPI GGTGA 6 cut(s) 65, 400, 742, 820, 1352, 1675
AvaI CYCGRG 1 cut(s) 318
AvaII GGWCC 1 cut(s) 1639
BalI TGGCCA 2 cut(s) 164, 989
BauI CACGAG 1 cut(s) 233
BbsI GAAGAC 2 cut(s) 762, 834
BbvCI CCTCAGC 1 cut(s) 1323
BccI CCATC 5 cut(s) 526, 730, 829, 1054, 1228
BceAI ACGGC 1 cut(s) 572
BciT130I CCWGG 2 cut(s) 38, 1439
BciVI GTATCC 1 cut(s) 649
BclI TGATCA 1 cut(s) 1444
BcnI CCSGG 2 cut(s) 85, 1580
BcoDI GTCTC 4 cut(s) 371, 574, 1217, 1713
BcuI ACTAGT 1 cut(s) 1207
BfaI CTAG 8 cut(s) 464, 474, 612, 935, 1208, 1599, 1634, 1894
BfrI CTTAAG 1 cut(s) 1346
BfuAI ACCTGC 1 cut(s) 1075
BfuI GTATCC 1 cut(s) 649
BglII AGATCT 1 cut(s) 903
BlpI GCTNAGC 1 cut(s) 1584
BmcAI AGTACT 1 cut(s) 1418
Bme1390I CCNGG 4 cut(s) 38, 85, 1439, 1580
Bme18I GGWCC 1 cut(s) 1639
BmeT110I CYCGRG 1 cut(s) 318
BmgT120I GGNCC 2 cut(s) 1602, 1639
BmiI GGNNCC 1 cut(s) 1577
BmrFI CCNGG 4 cut(s) 38, 85, 1439, 1580
BmsI GCATC 6 cut(s) 184, 774, 838, 964, 1309, 1844
BpiI GAAGAC 2 cut(s) 762, 834
BplI GAGNNNNNCTC 4 cut(s) 239, 271, 384, 416
BpmI CTGGAG 4 cut(s) 815, 1731, 1764, 1797
Bpu10I CCTNAGC 2 cut(s) 1323, 1697
Bpu1102I GCTNAGC 1 cut(s) 1584
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 2 cut(s) 85, 1580
BsaBI GATNNNNATC 1 cut(s) 978
BsaHI GRCGYC 1 cut(s) 839
BsaJI CCNNGG 2 cut(s) 345, 588
BsaWI WCCGGW 2 cut(s) 75, 1641
Bse1I ACTGG 2 cut(s) 32, 1342
Bse3DI GCAATG 2 cut(s) 1104, 1557
Bse8I GATNNNNATC 1 cut(s) 978
BseBI CCWGG 2 cut(s) 38, 1439
BseDI CCNNGG 2 cut(s) 345, 588
BseJI GATNNNNATC 1 cut(s) 978
BseMI GCAATG 2 cut(s) 1104, 1557
BseMII CTCAG 8 cut(s) 366, 395, 651, 1014, 1101, 1314, 1506, 1575
BseNI ACTGG 2 cut(s) 32, 1342
BseRI GAGGAG 2 cut(s) 749, 755
BsgI GTGCAG 1 cut(s) 197
BshFI GGCC 3 cut(s) 164, 989, 1604
BsiHKCI CYCGRG 1 cut(s) 318
BsiSI CCGG 5 cut(s) 76, 85, 93, 1579, 1642
BsmAI GTCTC 4 cut(s) 371, 574, 1217, 1713
BsnI GGCC 3 cut(s) 164, 989, 1604
BsoBI CYCGRG 1 cut(s) 318
Bsp143I GATC 8 cut(s) 70, 109, 364, 751, 903, 910, 1444, 1606
Bsp1720I GCTNAGC 1 cut(s) 1584
BspACI CCGC 7 cut(s) 245, 467, 506, 1064, 1500, 1734, 1862
BspANI GGCC 3 cut(s) 164, 989, 1604
BspCNI CTCAG 8 cut(s) 367, 394, 652, 1015, 1102, 1315, 1507, 1576
BspLI GGNNCC 1 cut(s) 1577
BspMI ACCTGC 1 cut(s) 1075
BspPI GGATC 3 cut(s) 372, 759, 918
BspQI GCTCTTC 4 cut(s) 546, 693, 1356, 1672
BspTI CTTAAG 1 cut(s) 1346
BsrDI GCAATG 2 cut(s) 1104, 1557
BsrI ACTGG 2 cut(s) 32, 1342
BssECI CCNNGG 2 cut(s) 345, 588
BssMI GATC 8 cut(s) 70, 109, 364, 751, 903, 910, 1444, 1606
BssNI GRCGYC 1 cut(s) 839
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 345
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 2 cut(s) 38, 1439
Bst4CI ACNGT 1 cut(s) 730
Bst6I CTCTTC 8 cut(s) 54, 546, 693, 882, 1356, 1368, 1672, 1716
BstACI GRCGYC 1 cut(s) 839
BstAFI CTTAAG 1 cut(s) 1346
BstAPI GCANNNNNTGC 2 cut(s) 194, 403
BstC8I GCNNGC 3 cut(s) 210, 504, 1590
BstDSI CCRYGG 1 cut(s) 588
BstHHI GCGC 5 cut(s) 325, 1084, 1271, 1567, 1696
BstKTI GATC 8 cut(s) 73, 112, 367, 754, 906, 913, 1447, 1609
BstMAI GTCTC 4 cut(s) 371, 574, 1217, 1713
BstMBI GATC 8 cut(s) 70, 109, 364, 751, 903, 910, 1444, 1606
BstNI CCWGG 2 cut(s) 38, 1439
BstNSI RCATGY 3 cut(s) 208, 410, 569
BstSCI CCNGG 4 cut(s) 36, 83, 1437, 1578
BstV2I GAAGAC 2 cut(s) 762, 834
BstX2I RGATCY 1 cut(s) 903
BstYI RGATCY 1 cut(s) 903
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 3 cut(s) 164, 989, 1604
BtgI CCRYGG 1 cut(s) 588
BtgZI GCGATG 1 cut(s) 866
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 3 cut(s) 210, 504, 1590
CfoI GCGC 5 cut(s) 325, 1084, 1271, 1567, 1696
Cfr13I GGNCC 2 cut(s) 1602, 1639
CseI GACGC 3 cut(s) 286, 479, 828
Csp6I GTAC 4 cut(s) 238, 1189, 1417, 1877
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
CviAII CATG 5 cut(s) 205, 403, 407, 451, 566
CviQI GTAC 4 cut(s) 238, 1189, 1417, 1877
DpnI GATC 8 cut(s) 72, 111, 366, 753, 905, 912, 1446, 1608
DpnII GATC 8 cut(s) 70, 109, 364, 751, 903, 910, 1444, 1606
EaeI YGGCCR 2 cut(s) 162, 987
Eam1104I CTCTTC 8 cut(s) 54, 546, 693, 882, 1356, 1368, 1672, 1716
EarI CTCTTC 8 cut(s) 54, 546, 693, 882, 1356, 1368, 1672, 1716
EciI GGCGGA 1 cut(s) 1079
Eco130I CCWWGG 1 cut(s) 345
Eco47I GGWCC 1 cut(s) 1639
Eco57I CTGAAG 5 cut(s) 773, 1019, 1176, 1380, 1477
Eco88I CYCGRG 1 cut(s) 318
EcoRII CCWGG 2 cut(s) 36, 1437
EcoT14I CCWWGG 1 cut(s) 345
EcoT22I ATGCAT 3 cut(s) 408, 1830, 1837
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 5 cut(s) 208, 406, 410, 454, 569
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FatI CATG 5 cut(s) 204, 402, 406, 450, 565
FauI CCCGC 1 cut(s) 1741
FauNDI CATATG 3 cut(s) 1738, 1771, 1804
FbaI TGATCA 1 cut(s) 1444
FspBI CTAG 8 cut(s) 464, 474, 612, 935, 1208, 1599, 1634, 1894
FspI TGCGCA 2 cut(s) 1083, 1566
GlaI GCGC 5 cut(s) 324, 1083, 1270, 1566, 1695
GsuI CTGGAG 4 cut(s) 815, 1731, 1764, 1797
HaeIII GGCC 3 cut(s) 164, 989, 1604
HapII CCGG 5 cut(s) 76, 85, 93, 1579, 1642
HgaI GACGC 3 cut(s) 286, 479, 828
HhaI GCGC 5 cut(s) 325, 1084, 1271, 1567, 1696
Hin1I GRCGYC 1 cut(s) 839
Hin1II CATG 5 cut(s) 208, 406, 410, 454, 569
Hin6I GCGC 5 cut(s) 323, 1082, 1269, 1565, 1694
HinP1I GCGC 5 cut(s) 323, 1082, 1269, 1565, 1694
HpaII CCGG 5 cut(s) 76, 85, 93, 1579, 1642
HphI GGTGA 6 cut(s) 65, 400, 742, 820, 1352, 1675
Hpy166II GTNNAC 2 cut(s) 240, 1343
Hpy188I TCNGA 9 cut(s) 376, 384, 439, 484, 574, 847, 910, 1111, 1516
Hpy188III TCNNGA 7 cut(s) 529, 692, 914, 1573, 1748, 1781, 1814
Hpy8I GTNNAC 2 cut(s) 240, 1343
Hpy99I CGWCG 1 cut(s) 302
HpyCH4III ACNGT 1 cut(s) 730
HpyCH4IV ACGT 1 cut(s) 1875
HpySE526I ACGT 1 cut(s) 1875
Hsp92I GRCGYC 1 cut(s) 839
Hsp92II CATG 5 cut(s) 208, 406, 410, 454, 569
HspAI GCGC 5 cut(s) 323, 1082, 1269, 1565, 1694
Ksp22I TGATCA 1 cut(s) 1444
Kzo9I GATC 8 cut(s) 70, 109, 364, 751, 903, 910, 1444, 1606
LguI GCTCTTC 4 cut(s) 546, 693, 1356, 1672
LmnI GCTCC 6 cut(s) 484, 508, 700, 762, 964, 1259
LweI GCATC 6 cut(s) 184, 774, 838, 964, 1309, 1844
MaeI CTAG 8 cut(s) 464, 474, 612, 935, 1208, 1599, 1634, 1894
MaeII ACGT 1 cut(s) 1875
MaeIII GTNAC 2 cut(s) 1390, 1871
MalI GATC 8 cut(s) 72, 111, 366, 753, 905, 912, 1446, 1608
MboI GATC 8 cut(s) 70, 109, 364, 751, 903, 910, 1444, 1606
MfeI CAATTG 2 cut(s) 24, 1865
MflI RGATCY 1 cut(s) 903
MlsI TGGCCA 2 cut(s) 164, 989
MluNI TGGCCA 2 cut(s) 164, 989
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 3 cut(s) 726, 1500, 1521
Mox20I TGGCCA 2 cut(s) 164, 989
Mph1103I ATGCAT 3 cut(s) 408, 1830, 1837
MroXI GAANNNNTTC 1 cut(s) 555
MscI TGGCCA 2 cut(s) 164, 989
MslI CAYNNNNRTG 1 cut(s) 782
Msp20I TGGCCA 2 cut(s) 164, 989
MspA1I CMGCKG 6 cut(s) 181, 245, 370, 793, 871, 1154
MspCI CTTAAG 1 cut(s) 1346
MspI CCGG 5 cut(s) 76, 85, 93, 1579, 1642
MspR9I CCNGG 4 cut(s) 38, 85, 1439, 1580
MunI CAATTG 2 cut(s) 24, 1865
MvaI CCWGG 2 cut(s) 38, 1439
NciI CCSGG 2 cut(s) 85, 1580
NdeI CATATG 3 cut(s) 1738, 1771, 1804
NdeII GATC 8 cut(s) 70, 109, 364, 751, 903, 910, 1444, 1606
NlaIII CATG 5 cut(s) 208, 406, 410, 454, 569
NlaIV GGNNCC 1 cut(s) 1577
NmeAIII GCCGAG 2 cut(s) 1246, 1639
NmuCI GTSAC 1 cut(s) 1871
NsbI TGCGCA 2 cut(s) 1083, 1566
NsiI ATGCAT 3 cut(s) 408, 1830, 1837
NspI RCATGY 3 cut(s) 208, 410, 569
PciI ACATGT 1 cut(s) 565
PciSI GCTCTTC 4 cut(s) 546, 693, 1356, 1672
PdmI GAANNNNTTC 1 cut(s) 555
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
PscI ACATGT 1 cut(s) 565
Psp6I CCWGG 2 cut(s) 36, 1437
PspGI CCWGG 2 cut(s) 36, 1437
PspN4I GGNNCC 1 cut(s) 1577
PspPI GGNCC 2 cut(s) 1602, 1639
PsuI RGATCY 1 cut(s) 903
PvuII CAGCTG 5 cut(s) 181, 370, 793, 871, 1154
RsaI GTAC 4 cut(s) 239, 1190, 1418, 1878
RsaNI GTAC 4 cut(s) 238, 1189, 1417, 1877
RseI CAYNNNNRTG 1 cut(s) 782
SapI GCTCTTC 4 cut(s) 546, 693, 1356, 1672
Sau3AI GATC 8 cut(s) 70, 109, 364, 751, 903, 910, 1444, 1606
Sau96I GGNCC 2 cut(s) 1602, 1639
ScaI AGTACT 1 cut(s) 1418
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 4 cut(s) 38, 85, 1439, 1580
SfaNI GCATC 6 cut(s) 184, 774, 838, 964, 1309, 1844
SinI GGWCC 1 cut(s) 1639
SmiMI CAYNNNNRTG 1 cut(s) 782
SmlI CTYRAG 2 cut(s) 218, 1346
SmoI CTYRAG 2 cut(s) 218, 1346
SpeI ACTAGT 1 cut(s) 1207
SsiI CCGC 7 cut(s) 245, 467, 506, 1064, 1500, 1734, 1862
SspI AATATT 1 cut(s) 713
SspMI CTAG 8 cut(s) 464, 474, 612, 935, 1208, 1599, 1634, 1894
StyD4I CCNGG 4 cut(s) 36, 83, 1437, 1578
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 730
TaiI ACGT 1 cut(s) 1878
TaqI TCGA 5 cut(s) 276, 300, 339, 708, 927
TatI WGTACW 2 cut(s) 237, 1416
TseFI GTSAC 1 cut(s) 1871
Tsp45I GTSAC 1 cut(s) 1871
Vha464I CTTAAG 1 cut(s) 1346
VpaK11BI GGWCC 1 cut(s) 1639
XapI RAATTY 2 cut(s) 1449, 1889
XceI RCATGY 3 cut(s) 208, 410, 569
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 8 cut(s) 464, 474, 612, 935, 1208, 1599, 1634, 1894
ZrmI AGTACT 1 cut(s) 1418
Zsp2I ATGCAT 3 cut(s) 408, 1830, 1837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.