Rh7BG049400

Protein WEAK CHLOROPLAST MOVEMENT UNDER BLUE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0015628
Physical Location & Seq
Forward (+)
3235699 .. 3237980
2282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG049400.1

Sequence Viewer

Length: 1803 bp
ATGGGTGAGGTTGATACCAAACCAATTGAATCAGTCCAGGCTGCTTTGTCCTTGTTTGAAGATAAAGCTGATCACCGGAAATCCCGCCCTACCGGCACCGATATGGAGAGTGGGAAAGAGAGAGAAATTGAAGGTATACTAAAGGATTTGGCCAATTACAAGGTGCAATTGGAAGCAAAGGATGCTGCCTATATGCAAGCTCTCCTTAAGCTGCAACACAATCAGTACACAGCAGATGAGCTTTCCACTCTGCTTCAAAGCATTGAGATTGAGAGAGATGAATACATACGAGACTGTAAAGAGGCTAGGACTCGCGTGGATGAACTCGAGTCCAAGGTGAAGGAGATGGCTGATCAGCTTTCGGAAACTGCCAAGCTACGCGAGCAGCTTATGCACGTTTTTAGCGAGTTGAAGGCCACACAAGCCGAGCTGTTTAGCATGGAAACTGACCTCACGCGTGCCAGAGATTCAGAGCTCAAGGCCCTAACACAAGCGGAACTGATGGAGAGTGCTTTCATCATGGAGCAAGAAAGGACAGAAGATCTTGTGAGACAGGTTGCAGATCTCAATGACGCGATATTCATGTCAAAAGATGCTGCCATTGAGGCCGAGAATGACAAGTTAGCAATGTTATCTGAGAAAGATGCCGAGATTGAGCTAGCTACACAAGCTGCTCTTAAAGCACAGGAGCAGCTAGAAGACGCAAGAATACAAACACTAGCAGTTCAGGAGTTGGAGAATCAGCTCCTTTCCAAGTCTGAGTACATTGAAATGCTTAAATATGAGATCAAAGATGTAAATGAACAACTTGTTTTGTCCAAGAAGGCTTCTGATGCTATTGATAATCTGAAGCTGGTACAAGAAGAGTTGGAAGGGATGGAAAGAAAGAACTTCGATCAGGAAGGTTTGATTGAGTCACTTGAAATGGAATTGAATCAGTTAAAACAAGAGATAATAAATGCTAATGAAGTGGCTAATCGCTTGAAGGTTGATGTTGAAATTCTAACTAGCGAGTTACAGGTGGCAAGAAGTGAGATTGATGAAATCAAGCAGAGGGAAAACAATGCACTAGTTGAGATAGCAATGCTGGAATCTGAGCTGCATAGAGGAAGGTCAAAAATTGCAGCAGCTGAGGCAGCTGAAGCACGAAAGGAGAACATGAAATCTGGGATGTATCTTGCAATTCAGCAACTAGCAATAGAAGCCGAAACTGCCAAGATGGAAAACAGAATGTTAAAACAAGGAGCAGATGGGGTAGAGGAAGAAGCTGATGAACAATATCAGGAGGCTGAAGCTTCCGAAATTGAAGAACTCAATGTTGAAGAAGATCATGAAGAGAGAAAAGAACAGGATAATGGACAACTTATAACAATTTCGTTGAAGGAATATGAGTGCTTGATTGAGAAGGCAGAGAAGGCTGATCAAATTCCTGTGTCATTGGAGAAGGAACTTAGTTTTGGTCGACAGTCAAGCTTAAGCGCCACAGAAGATAAGTCTGAGTTGGAAAGGTTGAGGAAGGAGCTAGAGGCTGCTTTGGAAAAAGTAGTGCAATTCAGGAACCGAGCAGAACAGGCAGTTACGAGGGCTGATCTTGCTGAGACAGCTAAAGCAGGACTCGAGGATCAGATAAGGCGATGGAGAGAGCACAGGCAAAAGAGAAAGGCTGCTCTTATTGCACTTAGGGAGGTAGCTGCTCCAAAAGAATATGGATATCATGCTCCTCAAGAATTTAGCCTTCCTGAATACAATGAAATACAAAAGGAAAATCCCCCACTAGGTCAGGTACTTAACTTGAAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WEMBL

Protein Analysis

600

Amino Acids

68.5

Weight (kDa)

4.64

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 3 - 433 4.9e-24 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14430
malus_domestica MD06G1198500.v1.1 MD14G1206000.v1.1
prunus_persica Prupe.5G199300_v2.0.a1 Prupe.5G199300_v2.0.a1
pyrus_communis pycom14g17040
rosa_chinensis RchiOBHm_Chr7g0182231
rosa_laevigata RLG00000005138
rosa_multiflora Rmu_sc0000003.1_g000012
rosa_roxburghii Rroxscaffold_3G00271400
rosa_rugosa Rorug06G0446300
rosa_samantha Rh7AG049300 Rh7BG049400 Rh7CG050700 Rh7DG048600
rosa_wichuraiana Rw7G004040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1367
AccB1I GGYRCC 1 cut(s) 95
AccI GTMKAC 2 cut(s) 136, 1462
AccII CGCG 4 cut(s) 315, 381, 457, 575
AciI CCGC 2 cut(s) 85, 494
AclWI GGATC 1 cut(s) 1629
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 3 cut(s) 999, 1425, 1727
AcuI CTGAAG 3 cut(s) 869, 1161, 1311
AfaI GTAC 4 cut(s) 227, 764, 858, 1785
AfiI CCNNNNNNNGG 1 cut(s) 1775
AflII CTTAAG 2 cut(s) 206, 1474
AflIII ACRYGT 1 cut(s) 455
AhlI ACTAGT 1 cut(s) 1069
AjnI CCWGG 1 cut(s) 36
AjuI GAANNNNNNNTTGG 2 cut(s) 1440, 1472
Alw21I GWGCWC 2 cut(s) 477, 1647
Alw26I GTCTC 3 cut(s) 285, 544, 1592
AlwI GGATC 1 cut(s) 1629
AlwNI CAGNNNCTG 1 cut(s) 1130
Ama87I CYCGRG 2 cut(s) 326, 1616
AoxI GGCC 4 cut(s) 150, 414, 480, 606
ApoI RAATTY 3 cut(s) 999, 1425, 1727
AspLEI GCGC 1 cut(s) 1481
AspS9I GGNCC 1 cut(s) 481
AsuHPI GGTGA 3 cut(s) 17, 65, 349
AsuNHI GCTAGC 1 cut(s) 658
AvaI CYCGRG 2 cut(s) 326, 1616
BalI TGGCCA 1 cut(s) 152
BanI GGYRCC 1 cut(s) 95
BanII GRGCYC 1 cut(s) 477
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 2 cut(s) 477, 1647
BbvCI CCTCAGC 1 cut(s) 1131
BccI CCATC 6 cut(s) 340, 496, 871, 1213, 1244, 1629
BciT130I CCWGG 1 cut(s) 38
BclI TGATCA 3 cut(s) 70, 352, 1420
BcoDI GTCTC 3 cut(s) 285, 544, 1592
BcuI ACTAGT 1 cut(s) 1069
BfoI RGCGCY 1 cut(s) 1482
BfrI CTTAAG 2 cut(s) 206, 1474
BglI GCCNNNNNGGC 2 cut(s) 93, 605
BglII AGATCT 2 cut(s) 541, 562
Bme1390I CCNGG 1 cut(s) 38
BmeT110I CYCGRG 2 cut(s) 326, 1616
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 2 cut(s) 97, 1559
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 4 cut(s) 172, 583, 634, 823
BmtI GCTAGC 1 cut(s) 662
BpiI GAAGAC 1 cut(s) 705
Bpu10I CCTNAGC 1 cut(s) 1131
BpuEI CTTGAG 2 cut(s) 461, 1707
BsaJI CCNNGG 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 75
Bsc4I CCNNNNNNNGG 1 cut(s) 1775
Bse118I RCCGGY 1 cut(s) 92
Bse3DI GCAATG 2 cut(s) 633, 1089
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 333
BseGI GGATG 4 cut(s) 187, 325, 882, 1176
BseLI CCNNNNNNNGG 1 cut(s) 1775
BseMI GCAATG 2 cut(s) 633, 1089
BseMII CTCAG 6 cut(s) 627, 750, 1086, 1122, 1488, 1587
BseRI GAGGAG 1 cut(s) 1710
Bsh1236I CGCG 4 cut(s) 315, 381, 457, 575
BshFI GGCC 4 cut(s) 152, 416, 482, 608
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 2 cut(s) 477, 1647
BsiHKCI CYCGRG 2 cut(s) 326, 1616
BsiSI CCGG 2 cut(s) 76, 93
BslI CCNNNNNNNGG 1 cut(s) 1775
BsmAI GTCTC 3 cut(s) 285, 544, 1592
BsnI GGCC 4 cut(s) 152, 416, 482, 608
BsoBI CYCGRG 2 cut(s) 326, 1616
Bsp1286I GDGCHC 2 cut(s) 477, 1647
BspACI CCGC 2 cut(s) 85, 494
BspANI GGCC 4 cut(s) 152, 416, 482, 608
BspCNI CTCAG 6 cut(s) 628, 751, 1087, 1123, 1489, 1588
BspFNI CGCG 4 cut(s) 315, 381, 457, 575
BspHI TCATGA 1 cut(s) 1330
BspLI GGNNCC 2 cut(s) 97, 1559
BspOI GCTAGC 1 cut(s) 662
BspPI GGATC 1 cut(s) 1629
BspT107I GGYRCC 1 cut(s) 95
BspTI CTTAAG 2 cut(s) 206, 1474
BsrDI GCAATG 2 cut(s) 633, 1089
BsrFI RCCGGY 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 333
BssNAI GTATAC 1 cut(s) 137
BssT1I CCWWGG 1 cut(s) 333
Bst1107I GTATAC 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 296, 1467
Bst6I CTCTTC 2 cut(s) 858, 1329
BstAFI CTTAAG 2 cut(s) 206, 1474
BstAPI GCANNNNNTGC 2 cut(s) 182, 391
BstC8I GCNNGC 4 cut(s) 198, 383, 459, 660
BstDEI CTNAG 8 cut(s) 636, 759, 1095, 1131, 1451, 1497, 1596, 1679
BstF5I GGATG 4 cut(s) 187, 325, 882, 1176
BstFNI CGCG 4 cut(s) 315, 381, 457, 575
BstH2I RGCGCY 1 cut(s) 1482
BstHHI GCGC 1 cut(s) 1481
BstMAI GTCTC 3 cut(s) 285, 544, 1592
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 36
BstUI CGCG 4 cut(s) 315, 381, 457, 575
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 2 cut(s) 541, 562
BstYI RGATCY 2 cut(s) 541, 562
BstZ17I GTATAC 1 cut(s) 137
BsuRI GGCC 4 cut(s) 152, 416, 482, 608
BtgZI GCGATG 1 cut(s) 1648
BtsCI GGATG 4 cut(s) 187, 325, 882, 1176
Cac8I GCNNGC 4 cut(s) 198, 383, 459, 660
CaiI CAGNNNCTG 1 cut(s) 1130
CciI TCATGA 1 cut(s) 1330
CfoI GCGC 1 cut(s) 1481
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 481
CseI GACGC 2 cut(s) 581, 710
Csp6I GTAC 4 cut(s) 226, 763, 857, 1784
CviAII CATG 6 cut(s) 439, 520, 583, 1159, 1331, 1715
CviQI GTAC 4 cut(s) 226, 763, 857, 1784
DdeI CTNAG 8 cut(s) 636, 759, 1095, 1131, 1451, 1497, 1596, 1679
EaeI YGGCCR 1 cut(s) 150
Eam1104I CTCTTC 2 cut(s) 858, 1329
EarI CTCTTC 2 cut(s) 858, 1329
Ecl136II GAGCTC 1 cut(s) 475
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 477
Eco32I GATATC 1 cut(s) 1712
Eco53kI GAGCTC 1 cut(s) 475
Eco57I CTGAAG 3 cut(s) 869, 1161, 1311
Eco88I CYCGRG 2 cut(s) 326, 1616
EcoICRI GAGCTC 1 cut(s) 475
EcoO109I RGGNCCY 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 36
EcoRV GATATC 1 cut(s) 1712
EcoT14I CCWWGG 1 cut(s) 333
EcoT38I GRGCYC 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 6 cut(s) 442, 523, 586, 1162, 1334, 1718
FalI AAGNNNNNCTT 4 cut(s) 189, 221, 660, 692
FatI CATG 6 cut(s) 438, 519, 582, 1158, 1330, 1714
FauI CCCGC 1 cut(s) 92
FbaI TGATCA 3 cut(s) 70, 352, 1420
FblI GTMKAC 2 cut(s) 136, 1462
FokI GGATG 4 cut(s) 194, 332, 889, 1183
FriOI GRGCYC 1 cut(s) 477
GlaI GCGC 1 cut(s) 1480
HaeII RGCGCY 1 cut(s) 1482
HaeIII GGCC 4 cut(s) 152, 416, 482, 608
HapII CCGG 2 cut(s) 76, 93
HgaI GACGC 2 cut(s) 581, 710
HhaI GCGC 1 cut(s) 1481
Hin1II CATG 6 cut(s) 442, 523, 586, 1162, 1334, 1718
Hin6I GCGC 1 cut(s) 1479
HinP1I GCGC 1 cut(s) 1479
HincII GTYRAC 1 cut(s) 1463
HindII GTYRAC 1 cut(s) 1463
HindIII AAGCTT 2 cut(s) 1293, 1471
HinfI GANTC 9 cut(s) 29, 310, 329, 467, 739, 914, 934, 1091, 1614
HpaII CCGG 2 cut(s) 76, 93
HphI GGTGA 3 cut(s) 17, 65, 349
Hpy166II GTNNAC 3 cut(s) 137, 228, 1463
Hpy188III TCNNGA 7 cut(s) 728, 899, 1283, 1331, 1555, 1724, 1739
Hpy8I GTNNAC 3 cut(s) 137, 228, 1463
HpyCH4III ACNGT 2 cut(s) 296, 1467
HpyCH4IV ACGT 1 cut(s) 396
HpyF3I CTNAG 8 cut(s) 636, 759, 1095, 1131, 1451, 1497, 1596, 1679
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 6 cut(s) 442, 523, 586, 1162, 1334, 1718
HspAI GCGC 1 cut(s) 1479
Ksp22I TGATCA 3 cut(s) 70, 352, 1420
LmnI GCTCC 7 cut(s) 523, 688, 750, 1244, 1519, 1699, 1723
LweI GCATC 4 cut(s) 172, 583, 634, 823
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 3 cut(s) 915, 1014, 1576
MfeI CAATTG 2 cut(s) 24, 167
MflI RGATCY 2 cut(s) 541, 562
MhlI GDGCHC 2 cut(s) 477, 1647
MlsI TGGCCA 1 cut(s) 152
MluI ACGCGT 1 cut(s) 455
MluNI TGGCCA 1 cut(s) 152
MlyI GAGTC 4 cut(s) 304, 338, 923, 1608
MmeI TCCRAC 3 cut(s) 714, 849, 1482
Mox20I TGGCCA 1 cut(s) 152
MscI TGGCCA 1 cut(s) 152
MseI TTAA 7 cut(s) 207, 678, 777, 941, 1235, 1475, 1788
MslI CAYNNNNRTG 2 cut(s) 101, 770
Msp20I TGGCCA 1 cut(s) 152
MspA1I CMGCKG 2 cut(s) 1130, 1139
MspCI CTTAAG 2 cut(s) 206, 1474
MspI CCGG 2 cut(s) 76, 93
MspR9I CCNGG 1 cut(s) 38
MunI CAATTG 2 cut(s) 24, 167
MvaI CCWGG 1 cut(s) 38
MvnI CGCG 4 cut(s) 315, 381, 457, 575
NheI GCTAGC 1 cut(s) 658
NlaIII CATG 6 cut(s) 442, 523, 586, 1162, 1334, 1718
NlaIV GGNNCC 2 cut(s) 97, 1559
NmeAIII GCCGAG 3 cut(s) 451, 634, 673
NmuCI GTSAC 1 cut(s) 915
PaeR7I CTCGAG 2 cut(s) 326, 1616
PagI TCATGA 1 cut(s) 1330
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PfeI GAWTC 5 cut(s) 29, 467, 739, 934, 1091
PleI GAGTC 4 cut(s) 304, 337, 922, 1608
PpsI GAGTC 4 cut(s) 304, 337, 922, 1608
PsiI TTATAA 1 cut(s) 1367
Psp124BI GAGCTC 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 97, 1559
PspPI GGNCC 1 cut(s) 481
PspXI VCTCGAGB 2 cut(s) 326, 1616
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 2 cut(s) 541, 562
PvuII CAGCTG 2 cut(s) 1130, 1139
RsaI GTAC 4 cut(s) 227, 764, 858, 1785
RsaNI GTAC 4 cut(s) 226, 763, 857, 1784
RseI CAYNNNNRTG 2 cut(s) 101, 770
SacI GAGCTC 1 cut(s) 477
SalI GTCGAC 1 cut(s) 1461
SaqAI TTAA 7 cut(s) 207, 678, 777, 941, 1235, 1475, 1788
Sau96I GGNCC 1 cut(s) 481
SchI GAGTC 4 cut(s) 304, 338, 923, 1608
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 2 cut(s) 477, 1647
SfaNI GCATC 4 cut(s) 172, 583, 634, 823
Sfr274I CTCGAG 2 cut(s) 326, 1616
SlaI CTCGAG 2 cut(s) 326, 1616
SmiMI CAYNNNNRTG 2 cut(s) 101, 770
SmlI CTYRAG 6 cut(s) 206, 326, 476, 1474, 1616, 1722
SmoI CTYRAG 6 cut(s) 206, 326, 476, 1474, 1616, 1722
SpeI ACTAGT 1 cut(s) 1069
SsiI CCGC 2 cut(s) 85, 494
SstI GAGCTC 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 2 cut(s) 296, 1467
TaiI ACGT 1 cut(s) 399
TaqI TCGA 4 cut(s) 327, 894, 1462, 1617
TatI WGTACW 2 cut(s) 225, 762
TfiI GAWTC 5 cut(s) 29, 467, 739, 934, 1091
Tru1I TTAA 7 cut(s) 207, 678, 777, 941, 1235, 1475, 1788
Tru9I TTAA 7 cut(s) 207, 678, 777, 941, 1235, 1475, 1788
TseFI GTSAC 1 cut(s) 915
Tsp45I GTSAC 1 cut(s) 915
Vha464I CTTAAG 2 cut(s) 206, 1474
XapI RAATTY 3 cut(s) 999, 1425, 1727
XhoI CTCGAG 2 cut(s) 326, 1616
XmiI GTMKAC 2 cut(s) 136, 1462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.