Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0010077

Physical Location & Seq

Genomic Coordinates Forward (+)

31055804 .. 31060763

Gene Structure 4960 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1229400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2928 bp

ATGACTGTCAAAACTCCTGGAACACCGGCTTCAAAAGTAGACAGGACACCAGTATCAACACCGGCTTCAAAAATAGACAGGACACCAGTCTCAACTCCGGGCGGGCCAAGAGCTAAGGAGGAGAAGATTGTTGTCACGGTGCGATTAAGGCCTCTAAACAAAAGAGAGCAACTAGCAAAAGACCAAGTTGCGTGGGAATGCCTCGATGACAACACGATTGTTTATAAGCCCCCGCCTCAAGAACGCTCAGTTCAACCTGCCCCATTCACATTTGATAAAGTTTTTGGTCCTACTTCCTTAACGGAAGCAGTATATGAGGAAGGAGTGAAAAATGTCGCCTTGTCTTCTTTGATGGGCATCAATGCAACTATATTCGCTTATGGGCAAACGAGCAGTGGGAAGACATACACAATGAGAGGGATAACAGAGAAAGCCGTCATTGATATCTACAATCATATAATCAATACTCCGGAGAGAGACTTTACAATAAAAATCTCTGGACTTGAAATATACAACGAGAATGTGAGGGACCTGCTAAATTCTGAATCAGGTCGTAGTTTGAAGCTTCTAGATGATCCAGAGGTGAAAGGTACCGTGGTTGAGAAGCTGGTGGAGGAAACTGCAAGCAATGATCAGCATTTGAGACATTTAATCAGCATTTGTGAAGCCCAAAGACAAGTTGGTGAAACTGCTTTAAATGATAACAGCTCCCGGTCTCATCAGATAATAAGACTGACAATTGAAAGTACACTCCGTGAGAATTCAGATTGCGTGAGATCTTTCGTGGCAAGCCTGAACTTTGTCGATCTGGCTGGAAGCGAAAGAGCTTCACAATCACTTGCAGATGGTGCTAGGCTCAGGGAAGGTTGCCACATCAACCTTAGCTTGATGACACTCACAACTGTCATTAGAAAGCTCAGCAGTGGGAAAAGAAGTGGTCATATACCTTATCGAGACTCAAAGCTCACTCGCATATTGCAGCATTCTCTAGGTGGGAATGCACGCACAGCCATCATATGCACCTTGAGTCCGGCATTGAGCCATTTTGAACAATCTCGCAATACGCTCTTCTTTGCCACCAGGGCAAAGGAAGTAACAAATAACGCTCGTGTGAATATGGTTGTTTCGGACAAACAGCTAGTGAAACATTTGCAGAAGGAAGTAGCGAGGCTGGAAGCAGAGCTGCGCACCCCTGACCCATTAAGGGAAAAAGAATTGAAAATTCAGCAGATGGAAATGGAGATTGAAGAATTGAGACGCCAAAGAGATCGTGCTCAGTCTCAGGTGCTTGAATTACAGCAAAAACTTCAGGAGGATCCACAGGGTTCAAACCCTTCTGGAATACCCCATCCCTCTGTGAAGAAATGTCTCTCATACACCGGTGTTCTATCAACAAAACCCGACAAAAAGGATATAGGCCCTGGTGATAGAGCAAGAAATTTGAGGCAGTCTATGAGGCAATCATCAACTGCTCCTTTCACACTAATGCATGAAATCCGCAAACTTGAACACCTGCAAGAACAGCTTGGGGAAGAAGCAAATCGAGCTCTGGAAGTATTACAAAAGGAGGTGGCTTGTCATAGGCTAGGCAACCAAGATGCAGCTGAAACAATTGCCAATCTGCAAGCGGAAATAAGAGAAATGCGTTCTGTCAGATCAGTGCCAAAAGAAGTTGAACTTGGAACTGTAGTTGCTACTAACAAGAGTGTAAGTGCTAATCTCAAGGAAGAGATTACGAGACTTCATTCACAGGGCAGCACGATTGAAAATCTCGAGGAACAGCTAGAAAGTGTTCAGAAGTCAATAGATAAATTGGTGATGTCTCTTCCTAGCAATTATCAAGAGTGTAATATTGAGGCATCCTCCAAGCCTAAGAAGGAGTCCAAAAAGAAGAAGTGTCTACCTTTGGCCTCAAGTAACATTGCCAACCGACAAAATTTCATCAGATCACCTTGCTCACCTCTATCAGCTTCTCGGCAAATTGCAGAATCTGAAACTGAAAATAGGGCTCCTGAGAATGATGATATTGTGTCCGGTGAGATTCAACCAGAGTCTGAGAAGGGGACTCCGACAAAGAATGAAGAATGTGGAGATGTCTCATCAAAGGAAAGCACTCCAACAGGTTATCGGCGTTCTAGTTCAGTAAACATGAAGAAAATGCAGAAGATGTTCCAAAATGCAGCAGAAGAGAACGTAAGAAACATTAGAACATATGTAACGGAACTGAAAGAGCGGGTGGCCAAACTGCAGTACCAAAAGCAGCTACTAGTTTGCCAGGTTCTGGAGCTGGAAGCAAATGAAGCTGCCGGGTACAATTTAGAGAATGACGAGGACACATGTGAGACTGAGGAGCCCCAAGTCTCGTGGCAAATAACTTTCAAGGAACAAAGACAGCAGATCATTGAGTTATGGGATTTGTGCTTTGTCTCCATCATTCATAGGACACAATTTTATTTGTTGTTTAAAGGTGACCCAGCTGATCAAATCTACATGGAAGTGGAGCTTAGACGGTTGACATGGCTGCAACAGCATCTCGCAGAACTTGGGGATGCTAGCCCTGCTCATGTGGGAGACGAACCCAGAGTTTCGTTGTCATCAAGCATGAGAGCGCTAAAGCGCGAAAGAGAGTTCCTTGCGAAGAGGTTGTCTTCACGCTTGACAGCAGAGGAGAGAGATGCATTGTACATGAAATGGGATGTCCCGCTTGAAGGAAAGCACAGGAAGCTGCAGTTTGTGAACAAGCTTTGGCAAGATCCCCATGATCCCAGGCACATCCAGGAGAGTGCTGAAGTAGTTGCAAAGCTTGTTGGCTTCTGCGAAAGTGGAAACCTGTCGAGAGAGATGTTTGAGCTCAATTTTGTGCTCCCATCTGATAGGAGGCCATGGATGATGGGCTGGAACCAGATTTCCAACCTTCTCAATTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000775 GO:0000910 GO:0000911 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007275 GO:0007349 GO:0008150 GO:0009524 GO:0009553 GO:0009555 GO:0009558 GO:0009561 GO:0009653 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022607 GO:0032501 GO:0032502 GO:0032506 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043515 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0048229 GO:0048285 GO:0048646 GO:0048856 GO:0051225 GO:0051301 GO:0061640 GO:0070925 GO:0071840 GO:0090307 GO:0098687 GO:0140014 GO:1902410 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3490 Kinesin

Protein Analysis

976

Amino Acids

110.22

Weight (kDa)

6.89

Isoelectric Point (pI)

56.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	46 - 179	3.4e-20	Microtubule binding
Kinesin	PF00225	48 - 365	3.4e-97	Kinesin motor domain
DUF3490	PF11995	793 - 955	2.1e-70	Domain of unknown function (DUF3490)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010077)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18370
fragaria_vesca	FvH4_5g11710 FvH4_5g11710 FvH4_5g11710 FvH4_5g11710
malus_domestica	MD06G1219700.v1.1 MD14G1229400.v1.1
prunus_persica	Prupe.5G224200_v2.0.a1
pyrus_communis	pycom06g19610 pycom14g19070
rosa_chinensis	RchiOBHm_Chr7g0186051
rosa_laevigata	RLG00000004869
rosa_multiflora	Rmu_co8507379.1_g000001 Rmu_sc0000194.1_g000022
rosa_roxburghii	Rroxscaffold_3G00268470
rosa_rugosa	Rorug06G0471900
rosa_samantha	Rh7AG070000 Rh7BG086000 Rh7CG078300 Rh7DG078300
rosa_wichuraiana	Rw7G006510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	225
AarI	CACCTGC	1 cut(s)	1521
Acc16I	TGCGCA	1 cut(s)	1187
Acc36I	ACCTGC	3 cut(s)	265, 540, 1521
Acc65I	GGTACC	1 cut(s)	590
AccB1I	GGYRCC	1 cut(s)	590
AccB7I	CCANNNNNTGG	1 cut(s)	2281
AccBSI	CCGCTC	1 cut(s)	2233
AccI	GTMKAC	2 cut(s)	39, 1900
AccII	CGCG	1 cut(s)	2619
AccIII	TCCGGA	1 cut(s)	469
AciI	CCGC	6 cut(s)	102, 233, 1498, 1628, 2233, 2702
AclWI	GGATC	5 cut(s)	569, 1310, 1323, 2747, 2756
AcoI	YGGCCR	1 cut(s)	2238
AcsI	RAATTY	5 cut(s)	538, 760, 1221, 1438, 1936
AcuI	CTGAAG	2 cut(s)	1292, 2808
AcyI	GRCGYC	1 cut(s)	1258
AdeI	CACNNNGTG	1 cut(s)	755
AfaI	GTAC	5 cut(s)	592, 748, 2252, 2312, 2684
AfeI	AGCGCT	1 cut(s)	2610
AfiI	CCNNNNNNNGG	3 cut(s)	1204, 2281, 2708
AflIII	ACRYGT	1 cut(s)	2336
AgeI	ACCGGT	1 cut(s)	1379
AhlI	ACTAGT	1 cut(s)	2266
AjnI	CCWGG	6 cut(s)	16, 1079, 1420, 2274, 2765, 2775
AjuI	GAANNNNNNNTTGG	2 cut(s)	1662, 1694
AloI	GAACNNNNNNTCC	2 cut(s)	2891, 2923
Alw21I	GWGCWC	4 cut(s)	1276, 1549, 2853, 2865
AlwI	GGATC	5 cut(s)	569, 1310, 1323, 2747, 2756
AlwNI	CAGNNNCTG	3 cut(s)	1991, 2054, 2281
Ama87I	CYCGRG	1 cut(s)	1772
Aor13HI	TCCGGA	1 cut(s)	469
Aor51HI	AGCGCT	1 cut(s)	2610
AoxI	GGCC	6 cut(s)	104, 149, 1417, 1908, 2238, 2879
ApeKI	GCWGC	9 cut(s)	979, 1183, 1601, 1755, 2180, 2260, 2303, 2521, 2725
ApoI	RAATTY	5 cut(s)	538, 760, 1221, 1438, 1936
ArsI	GACNNNNNNTTYG	2 cut(s)	2630, 2662
AsiGI	ACCGGT	1 cut(s)	1379
Asp700I	GAANNNNTTC	2 cut(s)	1791, 2168
Asp718I	GGTACC	1 cut(s)	590
AspLEI	GCGC	3 cut(s)	1188, 2611, 2619
AspS9I	GGNCC	4 cut(s)	104, 287, 529, 1418
AsuC2I	CCSGG	3 cut(s)	99, 712, 2308
AsuHPI	GGTGA	8 cut(s)	595, 695, 1436, 1828, 1941, 1950, 2048, 2480
AsuNHI	GCTAGC	1 cut(s)	2552
AvaI	CYCGRG	1 cut(s)	1772
AvaII	GGWCC	2 cut(s)	287, 529
BaeI	ACNNNNGTAYC	6 cut(s)	36, 69, 582, 615, 2234, 2267
BalI	TGGCCA	1 cut(s)	2240
BamHI	GGATCC	1 cut(s)	1315
BanI	GGYRCC	1 cut(s)	590
BanII	GRGCYC	4 cut(s)	1549, 2011, 2355, 2853
BauI	CACGAG	2 cut(s)	1107, 2362
BbsI	GAAGAC	3 cut(s)	336, 407, 2640
Bbv12I	GWGCWC	4 cut(s)	1276, 1549, 2853, 2865
BbvI	GCAGC	9 cut(s)	991, 1170, 1613, 1767, 2192, 2272, 2290, 2508, 2712
BccI	CCATC	8 cut(s)	346, 839, 1019, 1225, 1356, 2438, 2875, 2884
BceAI	ACGGC	1 cut(s)	419
BciT130I	CCWGG	6 cut(s)	18, 1081, 1422, 2276, 2767, 2777
BclI	TGATCA	2 cut(s)	631, 2479
BcnI	CCSGG	3 cut(s)	99, 712, 2308
BcuI	ACTAGT	1 cut(s)	2266
BfmI	CTRYAG	3 cut(s)	1686, 2246, 2726
BfoI	RGCGCY	1 cut(s)	2612
BfuAI	ACCTGC	3 cut(s)	265, 540, 1521
BglI	GCCNNNNNGGC	1 cut(s)	1082
BglII	AGATCT	1 cut(s)	776
BisI	GCNGC	9 cut(s)	980, 1184, 1602, 1756, 2181, 2261, 2304, 2522, 2726
BlpI	GCTNAGC	1 cut(s)	917
BlsI	GCNGC	9 cut(s)	981, 1185, 1603, 1757, 2182, 2262, 2305, 2523, 2727
Bme1390I	CCNGG	9 cut(s)	18, 99, 712, 1081, 1422, 2276, 2308, 2767, 2777
Bme18I	GGWCC	2 cut(s)	287, 529
BmeT110I	CYCGRG	1 cut(s)	1772
BmgT120I	GGNCC	4 cut(s)	104, 287, 529, 1418
BmiI	GGNNCC	6 cut(s)	530, 592, 1317, 2010, 2352, 2900
BmrFI	CCNGG	9 cut(s)	18, 99, 712, 1081, 1422, 2276, 2308, 2767, 2777
BmsI	GCATC	6 cut(s)	366, 1588, 1868, 2539, 2539, 2665
BmtI	GCTAGC	1 cut(s)	2556
BoxI	GACNNNNGTC	1 cut(s)	86
BpiI	GAAGAC	3 cut(s)	336, 407, 2640
BplI	GAGNNNNNCTC	2 cut(s)	1847, 1879
BpmI	CTGGAG	1 cut(s)	2303
Bpu10I	CCTNAGC	3 cut(s)	114, 857, 881
Bpu1102I	GCTNAGC	1 cut(s)	917
BpuEI	CTTGAG	4 cut(s)	222, 1045, 1706, 1897
BpuMI	CCSGG	3 cut(s)	99, 712, 2308
BsaBI	GATNNNNATC	1 cut(s)	356
BsaHI	GRCGYC	1 cut(s)	1258
BsaI	GGTCTC	1 cut(s)	720
BsaJI	CCNNGG	5 cut(s)	594, 1080, 1420, 2765, 2882
BsaWI	WCCGGW	3 cut(s)	469, 1379, 2033
Bsc4I	CCNNNNNNNGG	3 cut(s)	1204, 2281, 2708
Bse118I	RCCGGY	3 cut(s)	25, 61, 1379
Bse1I	ACTGG	2 cut(s)	50, 86
Bse3DI	GCAATG	2 cut(s)	634, 1920
Bse8I	GATNNNNATC	1 cut(s)	356
BseAI	TCCGGA	1 cut(s)	469
BseBI	CCWGG	6 cut(s)	18, 1081, 1422, 2276, 2767, 2777
BseDI	CCNNGG	5 cut(s)	594, 1080, 1420, 2765, 2882
BseGI	GGATG	6 cut(s)	1348, 1859, 2554, 2701, 2772, 2892
BseJI	GATNNNNATC	1 cut(s)	356
BseLI	CCNNNNNNNGG	3 cut(s)	1204, 2281, 2708
BseMI	GCAATG	2 cut(s)	634, 1920
BseMII	CTCAG	8 cut(s)	261, 871, 931, 1289, 1295, 2004, 2046, 2337
BseNI	ACTGG	2 cut(s)	50, 86
BseRI	GAGGAG	3 cut(s)	134, 2363, 2681
BseXI	GCAGC	9 cut(s)	991, 1170, 1613, 1767, 2192, 2272, 2290, 2508, 2712
BseYI	CCCAGC	1 cut(s)	2473
Bsh1236I	CGCG	1 cut(s)	2619
BshFI	GGCC	6 cut(s)	106, 151, 1419, 1910, 2240, 2881
BshNI	GGYRCC	1 cut(s)	590
BshTI	ACCGGT	1 cut(s)	1379
BsiHKAI	GWGCWC	4 cut(s)	1276, 1549, 2853, 2865
BsiHKCI	CYCGRG	1 cut(s)	1772
BsiSI	CCGG	9 cut(s)	26, 62, 98, 470, 712, 1031, 1380, 2034, 2307
BslFI	GGGAC	3 cut(s)	542, 2077, 2684
BslI	CCNNNNNNNGG	3 cut(s)	1204, 2281, 2708
BsmBI	CGTCTC	2 cut(s)	1249, 2565
BsmFI	GGGAC	3 cut(s)	542, 2077, 2684
BsmI	GAATGC	3 cut(s)	203, 982, 1003
BsnI	GGCC	6 cut(s)	106, 151, 1419, 1910, 2240, 2881
Bso31I	GGTCTC	1 cut(s)	720
BsoBI	CYCGRG	1 cut(s)	1772
Bsp1286I	GDGCHC	6 cut(s)	1276, 1549, 2011, 2355, 2853, 2865
Bsp13I	TCCGGA	1 cut(s)	469
Bsp1407I	TGTACA	1 cut(s)	2682
Bsp1720I	GCTNAGC	1 cut(s)	917
Bsp19I	CCATGG	1 cut(s)	2882
BspACI	CCGC	6 cut(s)	102, 233, 1498, 1628, 2233, 2702
BspANI	GGCC	6 cut(s)	106, 151, 1419, 1910, 2240, 2881
BspCNI	CTCAG	8 cut(s)	260, 870, 930, 1288, 1294, 2005, 2047, 2338
BspEI	TCCGGA	1 cut(s)	469
BspFNI	CGCG	1 cut(s)	2619
BspLI	GGNNCC	6 cut(s)	530, 592, 1317, 2010, 2352, 2900
BspMAI	CTGCAG	2 cut(s)	2250, 2730
BspMI	ACCTGC	3 cut(s)	265, 540, 1521
BspOI	GCTAGC	1 cut(s)	2556
BspPI	GGATC	5 cut(s)	569, 1310, 1323, 2747, 2756
BspQI	GCTCTTC	1 cut(s)	1073
BspT107I	GGYRCC	1 cut(s)	590
BspTNI	GGTCTC	1 cut(s)	720
BsrBI	CCGCTC	1 cut(s)	2233
BsrDI	GCAATG	2 cut(s)	634, 1920
BsrFI	RCCGGY	3 cut(s)	25, 61, 1379
BsrGI	TGTACA	1 cut(s)	2682
BsrI	ACTGG	2 cut(s)	50, 86
BssAI	RCCGGY	3 cut(s)	25, 61, 1379
BssECI	CCNNGG	5 cut(s)	594, 1080, 1420, 2765, 2882
BssNI	GRCGYC	1 cut(s)	1258
BssSI	CACGAG	2 cut(s)	1107, 2362
BssT1I	CCWWGG	1 cut(s)	2882
Bst2BI	CACGAG	2 cut(s)	1107, 2362
Bst2UI	CCWGG	6 cut(s)	18, 1081, 1422, 2276, 2767, 2777
Bst4CI	ACNGT	6 cut(s)	7, 139, 595, 904, 1687, 2511
Bst6I	CTCTTC	5 cut(s)	1073, 1722, 1830, 2181, 2633
BstACI	GRCGYC	1 cut(s)	1258
BstAPI	GCANNNNNTGC	1 cut(s)	848
BstAUI	TGTACA	1 cut(s)	2682
BstC8I	GCNNGC	6 cut(s)	104, 625, 790, 1003, 1626, 2554
BstDSI	CCRYGG	2 cut(s)	594, 2882
BstEII	GGTNACC	1 cut(s)	2468
BstF5I	GGATG	6 cut(s)	1348, 1859, 2554, 2701, 2772, 2892
BstFNI	CGCG	1 cut(s)	2619
BstH2I	RGCGCY	1 cut(s)	2612
BstHHI	GCGC	3 cut(s)	1188, 2611, 2619
BstNI	CCWGG	6 cut(s)	18, 1081, 1422, 2276, 2767, 2777
BstNSI	RCATGY	1 cut(s)	2340
BstPAI	GACNNNNGTC	1 cut(s)	86
BstPI	GGTNACC	1 cut(s)	2468
BstSCI	CCNGG	9 cut(s)	16, 97, 710, 1079, 1420, 2274, 2306, 2765, 2775
BstSFI	CTRYAG	3 cut(s)	1686, 2246, 2726
BstUI	CGCG	1 cut(s)	2619
BstV1I	GCAGC	9 cut(s)	991, 1170, 1613, 1767, 2192, 2272, 2290, 2508, 2712
BstV2I	GAAGAC	3 cut(s)	336, 407, 2640
BstX2I	RGATCY	3 cut(s)	776, 1315, 2752
BstYI	RGATCY	3 cut(s)	776, 1315, 2752
BsuRI	GGCC	6 cut(s)	106, 151, 1419, 1910, 2240, 2881
BtgI	CCRYGG	2 cut(s)	594, 2882
BtsCI	GGATG	6 cut(s)	1348, 1859, 2554, 2701, 2772, 2892
BtsI	GCAGTG	2 cut(s)	400, 928
BtsIMutI	CAGTG	3 cut(s)	400, 928, 1665
BveI	ACCTGC	3 cut(s)	265, 540, 1521
Cac8I	GCNNGC	6 cut(s)	104, 625, 790, 1003, 1626, 2554
CaiI	CAGNNNCTG	3 cut(s)	1991, 2054, 2281
CfoI	GCGC	3 cut(s)	1188, 2611, 2619
Cfr10I	RCCGGY	3 cut(s)	25, 61, 1379
Cfr13I	GGNCC	4 cut(s)	104, 287, 529, 1418
CseI	GACGC	1 cut(s)	1266
Csp6I	GTAC	5 cut(s)	591, 747, 2251, 2311, 2683
CspAI	ACCGGT	1 cut(s)	1379
CspCI	CAANNNNNGTGG	4 cut(s)	173, 208, 2345, 2380
CviQI	GTAC	5 cut(s)	591, 747, 2251, 2311, 2683
DraI	TTTAAA	2 cut(s)	696, 2464
DraIII	CACNNNGTG	1 cut(s)	755
EaeI	YGGCCR	1 cut(s)	2238
Eam1104I	CTCTTC	5 cut(s)	1073, 1722, 1830, 2181, 2633
EarI	CTCTTC	5 cut(s)	1073, 1722, 1830, 2181, 2633
Ecl136II	GAGCTC	2 cut(s)	1547, 2851
Eco130I	CCWWGG	1 cut(s)	2882
Eco147I	AGGCCT	1 cut(s)	151
Eco24I	GRGCYC	4 cut(s)	1549, 2011, 2355, 2853
Eco31I	GGTCTC	1 cut(s)	720
Eco32I	GATATC	1 cut(s)	445
Eco47I	GGWCC	2 cut(s)	287, 529
Eco47III	AGCGCT	1 cut(s)	2610
Eco53kI	GAGCTC	2 cut(s)	1547, 2851
Eco57I	CTGAAG	2 cut(s)	1292, 2808
Eco88I	CYCGRG	1 cut(s)	1772
Eco91I	GGTNACC	1 cut(s)	2468
EcoICRI	GAGCTC	2 cut(s)	1547, 2851
EcoO109I	RGGNCCY	2 cut(s)	529, 1418
EcoO65I	GGTNACC	1 cut(s)	2468
EcoRI	GAATTC	1 cut(s)	760
EcoRII	CCWGG	6 cut(s)	16, 1079, 1420, 2274, 2765, 2775
EcoRV	GATATC	1 cut(s)	445
EcoT14I	CCWWGG	1 cut(s)	2882
EcoT22I	ATGCAT	2 cut(s)	1491, 2680
EcoT38I	GRGCYC	4 cut(s)	1549, 2011, 2355, 2853
ErhI	CCWWGG	1 cut(s)	2882
Esp3I	CGTCTC	2 cut(s)	1249, 2565
FalI	AAGNNNNNCTT	6 cut(s)	1509, 1541, 1662, 1694, 2487, 2519
FaqI	GGGAC	3 cut(s)	542, 2077, 2684
FauI	CCCGC	4 cut(s)	95, 240, 2226, 2709
FauNDI	CATATG	2 cut(s)	1016, 2212
FbaI	TGATCA	2 cut(s)	631, 2479
FblI	GTMKAC	2 cut(s)	39, 1900
Fnu4HI	GCNGC	9 cut(s)	980, 1184, 1602, 1756, 2181, 2261, 2304, 2522, 2726
FokI	GGATG	6 cut(s)	1335, 1846, 2561, 2708, 2759, 2899
FriOI	GRGCYC	4 cut(s)	1549, 2011, 2355, 2853
Fsp4HI	GCNGC	9 cut(s)	980, 1184, 1602, 1756, 2181, 2261, 2304, 2522, 2726
FspI	TGCGCA	1 cut(s)	1187
GlaI	GCGC	3 cut(s)	1187, 2610, 2618
GluI	GCNGC	9 cut(s)	980, 1184, 1602, 1756, 2181, 2261, 2304, 2522, 2726
GsaI	CCCAGC	1 cut(s)	2477
GsuI	CTGGAG	1 cut(s)	2303
HaeII	RGCGCY	1 cut(s)	2612
HaeIII	GGCC	6 cut(s)	106, 151, 1419, 1910, 2240, 2881
HapII	CCGG	9 cut(s)	26, 62, 98, 470, 712, 1031, 1380, 2034, 2307
HgaI	GACGC	1 cut(s)	1266
HhaI	GCGC	3 cut(s)	1188, 2611, 2619
Hin1I	GRCGYC	1 cut(s)	1258
Hin6I	GCGC	3 cut(s)	1186, 2609, 2617
HinP1I	GCGC	3 cut(s)	1186, 2609, 2617
HincII	GTYRAC	1 cut(s)	2514
HindII	GTYRAC	1 cut(s)	2514
HindIII	AAGCTT	3 cut(s)	563, 2741, 2801
HinfI	GANTC	8 cut(s)	545, 956, 1027, 1880, 1988, 2041, 2051, 2065
HpaII	CCGG	9 cut(s)	26, 62, 98, 470, 712, 1031, 1380, 2034, 2307
HphI	GGTGA	8 cut(s)	595, 695, 1436, 1828, 1941, 1950, 2048, 2480
Hpy166II	GTNNAC	6 cut(s)	40, 749, 1901, 2146, 2514, 2737
Hpy8I	GTNNAC	6 cut(s)	40, 749, 1901, 2146, 2514, 2737
HpyAV	CCTTC	8 cut(s)	314, 857, 1150, 1344, 1870, 2053, 2702, 2924
HpyCH4III	ACNGT	6 cut(s)	7, 139, 595, 904, 1687, 2511
HpyCH4IV	ACGT	1 cut(s)	2193
HpySE526I	ACGT	1 cut(s)	2193
Hsp92I	GRCGYC	1 cut(s)	1258
HspAI	GCGC	3 cut(s)	1186, 2609, 2617
Kpn2I	TCCGGA	1 cut(s)	469
KpnI	GGTACC	1 cut(s)	594
Ksp22I	TGATCA	2 cut(s)	631, 2479
LguI	GCTCTTC	1 cut(s)	1073
LmnI	GCTCC	7 cut(s)	713, 1477, 2014, 2284, 2350, 2500, 2868
Lsp1109I	GCAGC	9 cut(s)	991, 1170, 1613, 1767, 2192, 2272, 2290, 2508, 2712
LweI	GCATC	6 cut(s)	366, 1588, 1868, 2539, 2539, 2665
MaeII	ACGT	1 cut(s)	2193
MaeIII	GTNAC	5 cut(s)	133, 1093, 1916, 2215, 2468
MbiI	CCGCTC	1 cut(s)	2233
MfeI	CAATTG	2 cut(s)	738, 1611
MflI	RGATCY	3 cut(s)	776, 1315, 2752
MhlI	GDGCHC	6 cut(s)	1276, 1549, 2011, 2355, 2853, 2865
MlsI	TGGCCA	1 cut(s)	2240
MluNI	TGGCCA	1 cut(s)	2240
MlyI	GAGTC	5 cut(s)	950, 1036, 1889, 2059, 2060
MmeI	TCCRAC	2 cut(s)	2093, 2141
Mox20I	TGGCCA	1 cut(s)	2240
Mph1103I	ATGCAT	2 cut(s)	1491, 2680
MroI	TCCGGA	1 cut(s)	469
MroXI	GAANNNNTTC	2 cut(s)	1791, 2168
MscI	TGGCCA	1 cut(s)	2240
MseI	TTAA	6 cut(s)	146, 299, 650, 695, 1202, 2463
MslI	CAYNNNNRTG	2 cut(s)	1484, 2495
Msp20I	TGGCCA	1 cut(s)	2240
MspA1I	CMGCKG	2 cut(s)	1604, 2477
MspI	CCGG	9 cut(s)	26, 62, 98, 470, 712, 1031, 1380, 2034, 2307
MspR9I	CCNGG	9 cut(s)	18, 99, 712, 1081, 1422, 2276, 2308, 2767, 2777
MunI	CAATTG	2 cut(s)	738, 1611
Mva1269I	GAATGC	3 cut(s)	203, 982, 1003
MvaI	CCWGG	6 cut(s)	18, 1081, 1422, 2276, 2767, 2777
MvnI	CGCG	1 cut(s)	2619
NciI	CCSGG	3 cut(s)	99, 712, 2308
NcoI	CCATGG	1 cut(s)	2882
NdeI	CATATG	2 cut(s)	1016, 2212
NheI	GCTAGC	1 cut(s)	2552
NlaIV	GGNNCC	6 cut(s)	530, 592, 1317, 2010, 2352, 2900
NmeAIII	GCCGAG	1 cut(s)	1954
NmuCI	GTSAC	2 cut(s)	133, 2468
NsbI	TGCGCA	1 cut(s)	1187
NsiI	ATGCAT	2 cut(s)	1491, 2680
NspI	RCATGY	1 cut(s)	2340
PaeR7I	CTCGAG	1 cut(s)	1772
PaqCI	CACCTGC	1 cut(s)	1521
PceI	AGGCCT	1 cut(s)	151
PciI	ACATGT	1 cut(s)	2336
PciSI	GCTCTTC	1 cut(s)	1073
PctI	GAATGC	3 cut(s)	203, 982, 1003
PdmI	GAANNNNTTC	2 cut(s)	1791, 2168
PfeI	GAWTC	3 cut(s)	545, 1988, 2041
PflMI	CCANNNNNTGG	1 cut(s)	2281
PfoI	TCCNGGA	2 cut(s)	16, 2775
PinAI	ACCGGT	1 cut(s)	1379
PkrI	GCNGC	9 cut(s)	981, 1185, 1603, 1757, 2182, 2262, 2305, 2523, 2727
PleI	GAGTC	5 cut(s)	950, 1035, 1888, 2059, 2059
PpsI	GAGTC	5 cut(s)	950, 1035, 1888, 2059, 2059
PpuMI	RGGWCCY	1 cut(s)	529
PscI	ACATGT	1 cut(s)	2336
PshAI	GACNNNNGTC	1 cut(s)	86
PsiI	TTATAA	1 cut(s)	225
Psp124BI	GAGCTC	2 cut(s)	1549, 2853
Psp5II	RGGWCCY	1 cut(s)	529
Psp6I	CCWGG	6 cut(s)	16, 1079, 1420, 2274, 2765, 2775
PspEI	GGTNACC	1 cut(s)	2468
PspFI	CCCAGC	1 cut(s)	2473
PspGI	CCWGG	6 cut(s)	16, 1079, 1420, 2274, 2765, 2775
PspN4I	GGNNCC	6 cut(s)	530, 592, 1317, 2010, 2352, 2900
PspPI	GGNCC	4 cut(s)	104, 287, 529, 1418
PspPPI	RGGWCCY	1 cut(s)	529
PstI	CTGCAG	2 cut(s)	2250, 2730
PstNI	CAGNNNCTG	3 cut(s)	1991, 2054, 2281
PsuI	RGATCY	3 cut(s)	776, 1315, 2752
PvuII	CAGCTG	2 cut(s)	1604, 2477
RsaI	GTAC	5 cut(s)	592, 748, 2252, 2312, 2684
RsaNI	GTAC	5 cut(s)	591, 747, 2251, 2311, 2683
RseI	CAYNNNNRTG	2 cut(s)	1484, 2495
SacI	GAGCTC	2 cut(s)	1549, 2853
SapI	GCTCTTC	1 cut(s)	1073
SaqAI	TTAA	6 cut(s)	146, 299, 650, 695, 1202, 2463
SatI	GCNGC	9 cut(s)	980, 1184, 1602, 1756, 2181, 2261, 2304, 2522, 2726
Sau96I	GGNCC	4 cut(s)	104, 287, 529, 1418
SchI	GAGTC	5 cut(s)	950, 1036, 1889, 2059, 2060
ScrFI	CCNGG	9 cut(s)	18, 99, 712, 1081, 1422, 2276, 2308, 2767, 2777
SduI	GDGCHC	6 cut(s)	1276, 1549, 2011, 2355, 2853, 2865
SfaNI	GCATC	6 cut(s)	366, 1588, 1868, 2539, 2539, 2665
SfcI	CTRYAG	3 cut(s)	1686, 2246, 2726
Sfr274I	CTCGAG	1 cut(s)	1772
SgrAI	CRCCGGYG	1 cut(s)	1379
SinI	GGWCC	2 cut(s)	287, 529
SlaI	CTCGAG	1 cut(s)	1772
SmiMI	CAYNNNNRTG	2 cut(s)	1484, 2495
SmlI	CTYRAG	5 cut(s)	237, 1024, 1721, 1772, 1912
SmoI	CTYRAG	5 cut(s)	237, 1024, 1721, 1772, 1912
SpeI	ACTAGT	1 cut(s)	2266
SseBI	AGGCCT	1 cut(s)	151
SsiI	CCGC	6 cut(s)	102, 233, 1498, 1628, 2233, 2702
SspI	AATATT	1 cut(s)	1852
SstI	GAGCTC	2 cut(s)	1549, 2853
StuI	AGGCCT	1 cut(s)	151
StyD4I	CCNGG	9 cut(s)	16, 97, 710, 1079, 1420, 2274, 2306, 2765, 2775
StyI	CCWWGG	1 cut(s)	2882
TaaI	ACNGT	6 cut(s)	7, 139, 595, 904, 1687, 2511
TaiI	ACGT	1 cut(s)	2196
TaqI	TCGA	6 cut(s)	204, 804, 952, 1543, 1773, 2834
TatI	WGTACW	2 cut(s)	746, 2682
TfiI	GAWTC	3 cut(s)	545, 1988, 2041
Tru1I	TTAA	6 cut(s)	146, 299, 650, 695, 1202, 2463
Tru9I	TTAA	6 cut(s)	146, 299, 650, 695, 1202, 2463
TscAI	CASTG	3 cut(s)	400, 928, 1665
TseFI	GTSAC	2 cut(s)	133, 2468
TseI	GCWGC	9 cut(s)	979, 1183, 1601, 1755, 2180, 2260, 2303, 2521, 2725
Tsp45I	GTSAC	2 cut(s)	133, 2468
TspDTI	ATGAA	8 cut(s)	1506, 1733, 1930, 2094, 2165, 2313, 2426, 2702
TspGWI	ACGGA	3 cut(s)	317, 743, 2234
TspRI	CASTG	3 cut(s)	400, 928, 1665
Van91I	CCANNNNNTGG	1 cut(s)	2281
VpaK11BI	GGWCC	2 cut(s)	287, 529
XapI	RAATTY	5 cut(s)	538, 760, 1221, 1438, 1936
XbaI	TCTAGA	1 cut(s)	568
XceI	RCATGY	1 cut(s)	2340
XcmI	CCANNNNNNNNNTGG	1 cut(s)	677
XhoI	CTCGAG	1 cut(s)	1772
XmiI	GTMKAC	2 cut(s)	39, 1900
XmnI	GAANNNNTTC	2 cut(s)	1791, 2168
Zsp2I	ATGCAT	2 cut(s)	1491, 2680

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.