Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0010077

Physical Location & Seq

Genomic Coordinates Forward (+)

5564027 .. 5568356

Gene Structure 4330 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G006510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2958 bp

ATGACGGTCAGAACTCCTGGAACCCCAGCTTCAAAAATTGACAAGACGCCAGTGACAACTCCGGCTTCGAAAATCGAGAGGACACCAGTGTCAACTCCAGGAGGGCCGAGAGCTAAGGAAGACAAGATTGTTCAATTGGCAAAAGACCAAGTGGCATGGGAATGCATCGATGATAACACCATTGTGTCGAAACCTGTGGACCGCTCAACTCAGCAGGCCTCATTTTCATTTGATAAAGTTTTCGGTCCAAATAGTATAACCGACACTGTATATGAGGAAGGAGTGAAGAATGTAGCCTTGTCTTCTTTGATGGGCATCAATGCTACTATATTTGCTTATGGACAAACTAGCAGTGGGAAGACGTACACAATGCGAGGAATAACAGAGAAAGCTGTCATTGATATCTACAACCATATAATGAATACTCCAGAGAGAGATTTTACAATAAAAATCTCCGGACTTGAAATATACAATGAGAATGTCAAGGACCTGTTAAATGCTGAATCTGGTCGTAATCTAAAGCTTCTAGATGATCCAGAGAAAGGTACTGTGGTTGAGAAACTGGTGGAGGAAACTGCAAGCAATGATCAGCACTTGAGACACTTAATCAGCATTTGTGAAGCTCAAAGACAAGTTGGTGAAACAGCTTTAAATGATAATAGCTCACGGTCACATCAGATAATAAGGCTGACAATTGAAAGTACACTCCGTGAAAATTCAGATTGTGAGATCTTTTGTTGCAAGCCTGTTGATTCGGTTATTCCTTTCTTGTTAATAACTTTCAACATCAACCTTCTGCAGAACTTTGTAGATCTGGCTGGAAGTGAACGAGCTTCACAGACACATGCAGATGGTGCTAGGCTAAGGGAAGGTTGCCATATCAACCTTAGCCTGATGACACTTACAACTGTTATCAGAAAGCTCAGTGTTGGAAAAAGAAGTGGCCATATACCCTACAGAGACTCAAAGCTCACTCGCATATTGCAGCACTCTCTTGGTGGGAATGCACGCACTGCTATCATATGTACCTTGAGTCCAGCAATGAGTCATCTTGAACAATCTCGGAATACTCTTTACTTTGCCACCAGGGCAAAGGAAGTCACAAATAATGCACGCGTGAACATGGTTGTTTCGGACAAGCAACTAGTGAAACATTTGCAGAAGGAAGTAGCTAGGCTGGAAGCAGAGCTGCGCACCCCTGCCACCCCAGATCCATCGAGTGAAAAAGATTTGAAAATTCAGCAGATGGAAATGGAAATTGAAGAATTGAAACGCCAGAGAGATATTGCTCAATCTCAAGTAATTGAACTACGCCAAAAGCTCAAGGAGGATCCGCAGGTTTCAAATCCATTGGAATTACCCCATCCTTCAGTGACTTCAGTGAAGAAATGTCTCTCCTACACTGGTGTTCTATCAACAAAACTTGATGGCAAGGAGCTAGGCCGTGGTGATAAAGCAAGAAATATAAAGTTGAGGCAGTCTATGAGGCAATCATCAGCTGCTCCTTTTACACTAATGCACGAAATCCGCAAACTTGAGCACCTGCAAGAGCAGCTAGGAGATGAAGCAAATCGAGCCCTGGAAGTATTACAAAAGGAGGTGGCTTGTCATAGACTAGGCAACCAAGATGCAGCTGAAACAATTGCTAACCTGCAAGCAGAAATAAGAGAAATGCAGTCAGTAAGATCAGTGCCAAAAGATGTTGATGTTGGAACTGTGGTTGCTACCAACAAGAGTGTCAGTGCTAATCTAAAGGAGGAGATCACAAGACTTCATTCACAGGGCACCACTATTGCAAATCTTGAGGAACAGCTTGAAAGTGTTCAGAAGTCTATAGATAAATTGGTGATGTCTCTTCCTAGCAATTTTGAACAATGCAATAATGAAGCATTCACCAAGTCTAAGAAGGAGTCCAAAAAGAAAAAGTTATCACCTTTAGCTTCAAGCAATGCTGCCAACCTGCCAAATTTTATCAGATCACCTTGCTCACCTCTATCAGCTTCTCGTCAAATTGTAGAGTCTGATGAAATTGAAAATAGAGTTCCTGAACATGATGATACTTTGTTCAGTGATATCCAACCAGAGTCTGATAAGGGGACTCCCACAAAGAGTGAAGATTGTGGAGATGTTTCGTCAAAGGAAAACACTCCATATAGGCCTTCTAGTTCGGTAAACATGAAGAAAATGCAGAAGATGTTCCAAAATGCAGCAGAAGAGAATGTAAGAAGCATAAGAACATATGTAACAGAGCTCAAAGAGCGGGTGGCCAAACTGCAGTACCAAAAGCAACTTCTAGTCTGCCAGGTTTTGGAGCTGGAAGCAAACGAAGCAGCTGGGTATGAGATGGAGGATGATGAAATCACATGCGAGCCAGAGGAGCCCCAGGTCCCATGGCAGATAACTTTCAAGGATCAAAGGCAGCAAATCATTGAGTTATGGGATTTGTGCTATGTCTCTATCATCCATAGGACACAGTTTTATTTGTTATTCAAGGGAGATCCAGCTGATCAAATCTATATGGAAGTGGAGCTCAGGCGATTGACATGGTTGCAAGAGCATCTGGCAGAACTTGGTAATGCTAGCCCTGCTCCTCATGTGGGAGACGAGCCAGCAGTCTCTTTGTCATCAAGTATCCGAGCACTAAGGCGTGAAAGGGAGTTCCTTGCCAAGAGGGTAACCTCACGTTTGAGCGCAGAGGAGAGAGATGCATTGTACATCAAATGGGATGTTCCACTTGAAGGGAAGCAGAGGAGGATGCAGTTTGTGAATAAGCTCTGGACCGACCCTCATGATGCCAAGCATATACAGGAGAGTGCTGAGATAGTTGCAAAGCTTGTTGGTTTCTGCGAAAGCGGCAACATGCCAAAGGAGATGTTTGAGCTGAATTTTGCACTTCCATCTGATAGGAGGCCATGGATTATGGGATGGAACCCAATTTCAAACCTTCTTAACATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000775 GO:0000910 GO:0000911 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007275 GO:0007349 GO:0008150 GO:0009524 GO:0009553 GO:0009555 GO:0009558 GO:0009561 GO:0009653 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022607 GO:0032501 GO:0032502 GO:0032506 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043515 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0048229 GO:0048285 GO:0048646 GO:0048856 GO:0051225 GO:0051301 GO:0061640 GO:0070925 GO:0071840 GO:0090307 GO:0098687 GO:0140014 GO:1902410 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3490 Kinesin

Protein Analysis

985

Amino Acids

110.95

Weight (kDa)

6.06

Isoelectric Point (pI)

49.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	55 - 367	1.6e-92	Kinesin motor domain
Microtub_bd	PF16796	58 - 165	7.5e-21	Microtubule binding
DUF3490	PF11995	802 - 965	8.8e-73	Domain of unknown function (DUF3490)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010077)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18370
fragaria_vesca	FvH4_5g11710 FvH4_5g11710 FvH4_5g11710 FvH4_5g11710
malus_domestica	MD06G1219700.v1.1 MD14G1229400.v1.1
prunus_persica	Prupe.5G224200_v2.0.a1
pyrus_communis	pycom06g19610 pycom14g19070
rosa_chinensis	RchiOBHm_Chr7g0186051
rosa_laevigata	RLG00000004869
rosa_multiflora	Rmu_co8507379.1_g000001 Rmu_sc0000194.1_g000022
rosa_roxburghii	Rroxscaffold_3G00268470
rosa_rugosa	Rorug06G0471900
rosa_samantha	Rh7AG070000 Rh7BG086000 Rh7CG078300 Rh7DG078300
rosa_wichuraiana	Rw7G006510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1551
AasI	GACNNNNNNGTC	1 cut(s)	88
Acc16I	TGCGCA	1 cut(s)	1193
Acc36I	ACCTGC	4 cut(s)	1327, 1551, 1659, 1968
AccB1I	GGYRCC	1 cut(s)	1784
AccB7I	CCANNNNNTGG	1 cut(s)	2308
AccBSI	CCGCTC	2 cut(s)	204, 2260
AccII	CGCG	1 cut(s)	1116
AccIII	TCCGGA	1 cut(s)	455
AciI	CCGC	5 cut(s)	202, 1334, 1528, 2260, 2853
AclWI	GGATC	6 cut(s)	527, 1205, 1325, 1338, 2418, 2492
AcoI	YGGCCR	2 cut(s)	943, 2265
AcsI	RAATTY	4 cut(s)	715, 1236, 1966, 2884
AcuI	CTGAAG	2 cut(s)	1353, 1362
AcyI	GRCGYC	1 cut(s)	47
AdeI	CACNNNGTG	1 cut(s)	710
AfaI	GTAC	6 cut(s)	365, 547, 703, 1027, 2279, 2714
AfiI	CCNNNNNNNGG	4 cut(s)	542, 2308, 2597, 2738
AflIII	ACRYGT	2 cut(s)	1114, 2952
AhlI	ACTAGT	1 cut(s)	1144
AjnI	CCWGG	6 cut(s)	16, 97, 1085, 1578, 2301, 2382
AjuI	GAANNNNNNNTTGG	2 cut(s)	2274, 2306
AleI	CACNNNNGTG	1 cut(s)	182
Alw21I	GWGCWC	4 cut(s)	1542, 2253, 2532, 2641
Alw26I	GTCTC	7 cut(s)	592, 954, 1397, 1857, 2458, 2595, 2620
AlwI	GGATC	6 cut(s)	527, 1205, 1325, 1338, 2418, 2492
AlwNI	CAGNNNCTG	1 cut(s)	2087
Aor13HI	TCCGGA	1 cut(s)	455
AoxI	GGCC	7 cut(s)	104, 216, 943, 1441, 2156, 2265, 2909
ApeKI	GCWGC	9 cut(s)	985, 1189, 1499, 1552, 1631, 1952, 2207, 2330, 2419
ApoI	RAATTY	4 cut(s)	715, 1236, 1966, 2884
Asp700I	GAANNNNTTC	2 cut(s)	1821, 2195
AspLEI	GCGC	2 cut(s)	1194, 2693
AspS9I	GGNCC	6 cut(s)	104, 199, 245, 487, 2386, 2778
AsuHPI	GGTGA	7 cut(s)	650, 1460, 1858, 1885, 1923, 1971, 1980
AsuII	TTCGAA	1 cut(s)	68
AsuNHI	GCTAGC	1 cut(s)	2579
AvaII	GGWCC	5 cut(s)	199, 245, 487, 2386, 2778
BaeGI	GKGCMC	1 cut(s)	1787
BaeI	ACNNNNGTAYC	4 cut(s)	537, 570, 2261, 2294
BalI	TGGCCA	2 cut(s)	945, 2267
BamHI	GGATCC	1 cut(s)	1330
BanI	GGYRCC	1 cut(s)	1784
BanII	GRGCYC	4 cut(s)	1579, 2253, 2382, 2532
BbsI	GAAGAC	3 cut(s)	126, 294, 365
Bbv12I	GWGCWC	4 cut(s)	1542, 2253, 2532, 2641
BbvI	GCAGC	9 cut(s)	997, 1176, 1486, 1564, 1643, 1939, 2219, 2342, 2431
BccI	CCATC	9 cut(s)	304, 845, 1222, 1240, 1371, 1421, 2338, 2905, 2919
BceAI	ACGGC	1 cut(s)	1428
BciT130I	CCWGG	6 cut(s)	18, 99, 1087, 1580, 2303, 2384
BciVI	GTATCC	1 cut(s)	2642
BclI	TGATCA	2 cut(s)	586, 2506
BcoDI	GTCTC	7 cut(s)	592, 954, 1397, 1857, 2458, 2595, 2620
BcuI	ACTAGT	1 cut(s)	1144
BfmI	CTRYAG	4 cut(s)	797, 955, 1833, 2273
BfuAI	ACCTGC	4 cut(s)	1327, 1551, 1659, 1968
BfuI	GTATCC	1 cut(s)	2642
BglI	GCCNNNNNGGC	1 cut(s)	1088
BglII	AGATCT	2 cut(s)	729, 811
Bme1390I	CCNGG	6 cut(s)	18, 99, 1087, 1580, 2303, 2384
Bme18I	GGWCC	5 cut(s)	199, 245, 487, 2386, 2778
BmgT120I	GGNCC	6 cut(s)	104, 199, 245, 487, 2386, 2778
BmiI	GGNNCC	6 cut(s)	22, 1332, 1786, 2379, 2388, 2930
BmrFI	CCNGG	6 cut(s)	18, 99, 1087, 1580, 2303, 2384
BmsI	GCATC	7 cut(s)	174, 324, 1618, 2566, 2695, 2745, 2782
BmtI	GCTAGC	1 cut(s)	2583
BpiI	GAAGAC	3 cut(s)	126, 294, 365
BpmI	CTGGAG	2 cut(s)	81, 411
Bpu10I	CCTNAGC	4 cut(s)	114, 863, 887, 2531
Bpu14I	TTCGAA	1 cut(s)	68
BpuEI	CTTGAG	6 cut(s)	616, 1051, 1281, 1307, 1556, 1823
Bsa29I	ATCGAT	1 cut(s)	168
BsaBI	GATNNNNATC	1 cut(s)	314
BsaHI	GRCGYC	1 cut(s)	47
BsaJI	CCNNGG	6 cut(s)	1086, 1444, 1578, 2382, 2390, 2912
BsaWI	WCCGGW	1 cut(s)	455
Bsc4I	CCNNNNNNNGG	4 cut(s)	542, 2308, 2597, 2738
Bse1I	ACTGG	4 cut(s)	50, 86, 567, 1408
Bse3DI	GCAATG	3 cut(s)	589, 1047, 1954
Bse8I	GATNNNNATC	1 cut(s)	314
BseAI	TCCGGA	1 cut(s)	455
BseBI	CCWGG	6 cut(s)	18, 99, 1087, 1580, 2303, 2384
BseCI	ATCGAT	1 cut(s)	168
BseDI	CCNNGG	6 cut(s)	1086, 1444, 1578, 2382, 2390, 2912
BseGI	GGATG	6 cut(s)	1363, 2356, 2460, 2731, 2760, 2930
BseJI	GATNNNNATC	1 cut(s)	314
BseLI	CCNNNNNNNGG	4 cut(s)	542, 2308, 2597, 2738
BseMI	GCAATG	3 cut(s)	589, 1047, 1954
BseMII	CTCAG	4 cut(s)	224, 937, 2545, 2808
BseNI	ACTGG	4 cut(s)	50, 86, 567, 1408
BseRI	GAGGAG	5 cut(s)	1772, 2390, 2580, 2711, 2764
BseSI	GKGCMC	1 cut(s)	1787
BseXI	GCAGC	9 cut(s)	997, 1176, 1486, 1564, 1643, 1939, 2219, 2342, 2431
BseYI	CCCAGC	2 cut(s)	25, 2333
Bsh1236I	CGCG	1 cut(s)	1116
BshFI	GGCC	7 cut(s)	106, 218, 945, 1443, 2158, 2267, 2911
BshNI	GGYRCC	1 cut(s)	1784
BshVI	ATCGAT	1 cut(s)	168
BsiHKAI	GWGCWC	4 cut(s)	1542, 2253, 2532, 2641
BsiSI	CCGG	2 cut(s)	62, 456
BslFI	GGGAC	2 cut(s)	2110, 2372
BslI	CCNNNNNNNGG	4 cut(s)	542, 2308, 2597, 2738
BsmAI	GTCTC	7 cut(s)	592, 954, 1397, 1857, 2458, 2595, 2620
BsmBI	CGTCTC	1 cut(s)	2595
BsmFI	GGGAC	2 cut(s)	2110, 2372
BsmI	GAATGC	3 cut(s)	167, 1009, 1889
BsnI	GGCC	7 cut(s)	106, 218, 945, 1443, 2158, 2267, 2911
Bsp119I	TTCGAA	1 cut(s)	68
Bsp1286I	GDGCHC	7 cut(s)	1542, 1579, 1787, 2253, 2382, 2532, 2641
Bsp13I	TCCGGA	1 cut(s)	455
Bsp1407I	TGTACA	1 cut(s)	2712
Bsp19I	CCATGG	2 cut(s)	2390, 2912
BspACI	CCGC	5 cut(s)	202, 1334, 1528, 2260, 2853
BspANI	GGCC	7 cut(s)	106, 218, 945, 1443, 2158, 2267, 2911
BspCNI	CTCAG	4 cut(s)	223, 936, 2544, 2809
BspDI	ATCGAT	1 cut(s)	168
BspEI	TCCGGA	1 cut(s)	455
BspFNI	CGCG	1 cut(s)	1116
BspHI	TCATGA	1 cut(s)	2788
BspLI	GGNNCC	6 cut(s)	22, 1332, 1786, 2379, 2388, 2930
BspMAI	CTGCAG	2 cut(s)	801, 2277
BspMI	ACCTGC	4 cut(s)	1327, 1551, 1659, 1968
BspOI	GCTAGC	1 cut(s)	2583
BspPI	GGATC	6 cut(s)	527, 1205, 1325, 1338, 2418, 2492
BspT104I	TTCGAA	1 cut(s)	68
BspT107I	GGYRCC	1 cut(s)	1784
BsrBI	CCGCTC	2 cut(s)	204, 2260
BsrDI	GCAATG	3 cut(s)	589, 1047, 1954
BsrGI	TGTACA	1 cut(s)	2712
BsrI	ACTGG	4 cut(s)	50, 86, 567, 1408
BssECI	CCNNGG	6 cut(s)	1086, 1444, 1578, 2382, 2390, 2912
BssNI	GRCGYC	1 cut(s)	47
BssT1I	CCWWGG	2 cut(s)	2390, 2912
Bst2UI	CCWGG	6 cut(s)	18, 99, 1087, 1580, 2303, 2384
Bst4CI	ACNGT	7 cut(s)	7, 268, 550, 669, 910, 1717, 2475
Bst6I	CTCTTC	2 cut(s)	1860, 2208
BstACI	GRCGYC	1 cut(s)	47
BstAPI	GCANNNNNTGC	2 cut(s)	854, 1013
BstAUI	TGTACA	1 cut(s)	2712
BstBI	TTCGAA	1 cut(s)	68
BstC8I	GCNNGC	9 cut(s)	216, 580, 743, 1009, 1114, 1656, 2369, 2581, 2610
BstDEI	CTNAG	9 cut(s)	114, 210, 863, 887, 923, 1902, 2531, 2642, 2817
BstDSI	CCRYGG	3 cut(s)	1444, 2390, 2912
BstEII	GGTNACC	1 cut(s)	2674
BstF5I	GGATG	6 cut(s)	1363, 2356, 2460, 2731, 2760, 2930
BstFNI	CGCG	1 cut(s)	1116
BstHHI	GCGC	2 cut(s)	1194, 2693
BstMAI	GTCTC	7 cut(s)	592, 954, 1397, 1857, 2458, 2595, 2620
BstNI	CCWGG	6 cut(s)	18, 99, 1087, 1580, 2303, 2384
BstNSI	RCATGY	4 cut(s)	848, 2367, 2863, 2956
BstPI	GGTNACC	1 cut(s)	2674
BstSCI	CCNGG	6 cut(s)	16, 97, 1085, 1578, 2301, 2382
BstSFI	CTRYAG	4 cut(s)	797, 955, 1833, 2273
BstSLI	GKGCMC	1 cut(s)	1787
BstUI	CGCG	1 cut(s)	1116
BstV1I	GCAGC	9 cut(s)	997, 1176, 1486, 1564, 1643, 1939, 2219, 2342, 2431
BstV2I	GAAGAC	3 cut(s)	126, 294, 365
BstX2I	RGATCY	5 cut(s)	729, 811, 1210, 1330, 2497
BstYI	RGATCY	5 cut(s)	729, 811, 1210, 1330, 2497
Bsu15I	ATCGAT	1 cut(s)	168
BsuI	GTATCC	1 cut(s)	2642
BsuRI	GGCC	7 cut(s)	106, 218, 945, 1443, 2158, 2267, 2911
BsuTUI	ATCGAT	1 cut(s)	168
BtgI	CCRYGG	3 cut(s)	1444, 2390, 2912
BtsCI	GGATG	6 cut(s)	1363, 2356, 2460, 2731, 2760, 2930
BtsI	GCAGTG	2 cut(s)	358, 1011
BveI	ACCTGC	4 cut(s)	1327, 1551, 1659, 1968
Cac8I	GCNNGC	9 cut(s)	216, 580, 743, 1009, 1114, 1656, 2369, 2581, 2610
CaiI	CAGNNNCTG	1 cut(s)	2087
CciI	TCATGA	1 cut(s)	2788
CfoI	GCGC	2 cut(s)	1194, 2693
Cfr13I	GGNCC	6 cut(s)	104, 199, 245, 487, 2386, 2778
ClaI	ATCGAT	1 cut(s)	168
CseI	GACGC	1 cut(s)	55
Csp6I	GTAC	6 cut(s)	364, 546, 702, 1026, 2278, 2713
CviQI	GTAC	6 cut(s)	364, 546, 702, 1026, 2278, 2713
DdeI	CTNAG	9 cut(s)	114, 210, 863, 887, 923, 1902, 2531, 2642, 2817
DraI	TTTAAA	1 cut(s)	651
DraIII	CACNNNGTG	1 cut(s)	710
DrdI	GACNNNNNNGTC	1 cut(s)	88
DseDI	GACNNNNNNGTC	1 cut(s)	88
EaeI	YGGCCR	2 cut(s)	943, 2265
Eam1104I	CTCTTC	2 cut(s)	1860, 2208
EarI	CTCTTC	2 cut(s)	1860, 2208
Ecl136II	GAGCTC	2 cut(s)	2251, 2530
Eco130I	CCWWGG	2 cut(s)	2390, 2912
Eco147I	AGGCCT	2 cut(s)	218, 2158
Eco24I	GRGCYC	4 cut(s)	1579, 2253, 2382, 2532
Eco32I	GATATC	2 cut(s)	403, 2074
Eco47I	GGWCC	5 cut(s)	199, 245, 487, 2386, 2778
Eco53kI	GAGCTC	2 cut(s)	2251, 2530
Eco57I	CTGAAG	2 cut(s)	1353, 1362
Eco91I	GGTNACC	1 cut(s)	2674
EcoICRI	GAGCTC	2 cut(s)	2251, 2530
EcoO109I	RGGNCCY	2 cut(s)	487, 2386
EcoO65I	GGTNACC	1 cut(s)	2674
EcoRII	CCWGG	6 cut(s)	16, 97, 1085, 1578, 2301, 2382
EcoRV	GATATC	2 cut(s)	403, 2074
EcoT14I	CCWWGG	2 cut(s)	2390, 2912
EcoT22I	ATGCAT	2 cut(s)	167, 2710
EcoT38I	GRGCYC	4 cut(s)	1579, 2253, 2382, 2532
ErhI	CCWWGG	2 cut(s)	2390, 2912
Esp3I	CGTCTC	1 cut(s)	2595
FaqI	GGGAC	2 cut(s)	2110, 2372
FauI	CCCGC	1 cut(s)	2253
FauNDI	CATATG	2 cut(s)	1022, 2239
FbaI	TGATCA	2 cut(s)	586, 2506
FokI	GGATG	6 cut(s)	1350, 2363, 2447, 2738, 2767, 2937
FriOI	GRGCYC	4 cut(s)	1579, 2253, 2382, 2532
FspI	TGCGCA	1 cut(s)	1193
GlaI	GCGC	2 cut(s)	1193, 2692
GsaI	CCCAGC	2 cut(s)	29, 2337
GsuI	CTGGAG	2 cut(s)	81, 411
HaeIII	GGCC	7 cut(s)	106, 218, 945, 1443, 2158, 2267, 2911
HapII	CCGG	2 cut(s)	62, 456
HgaI	GACGC	1 cut(s)	55
HhaI	GCGC	2 cut(s)	1194, 2693
Hin1I	GRCGYC	1 cut(s)	47
Hin6I	GCGC	2 cut(s)	1192, 2691
HinP1I	GCGC	2 cut(s)	1192, 2691
HincII	GTYRAC	1 cut(s)	93
HindII	GTYRAC	1 cut(s)	93
HindIII	AAGCTT	2 cut(s)	521, 2831
HinfI	GANTC	9 cut(s)	503, 752, 962, 1033, 1045, 1910, 2018, 2084, 2098
HpaII	CCGG	2 cut(s)	62, 456
HphI	GGTGA	7 cut(s)	650, 1460, 1858, 1885, 1923, 1971, 1980
Hpy166II	GTNNAC	7 cut(s)	93, 199, 366, 704, 827, 1120, 2173
Hpy8I	GTNNAC	7 cut(s)	93, 199, 366, 704, 827, 1120, 2173
HpyAV	CCTTC	9 cut(s)	272, 803, 863, 1156, 1377, 1900, 2169, 2732, 2954
HpyCH4III	ACNGT	7 cut(s)	7, 268, 550, 669, 910, 1717, 2475
HpyCH4IV	ACGT	2 cut(s)	362, 2683
HpyF3I	CTNAG	9 cut(s)	114, 210, 863, 887, 923, 1902, 2531, 2642, 2817
HpySE526I	ACGT	2 cut(s)	362, 2683
Hsp92I	GRCGYC	1 cut(s)	47
HspAI	GCGC	2 cut(s)	1192, 2691
Kpn2I	TCCGGA	1 cut(s)	455
Ksp22I	TGATCA	2 cut(s)	586, 2506
LmnI	GCTCC	6 cut(s)	1435, 1507, 2311, 2377, 2527, 2593
Lsp1109I	GCAGC	9 cut(s)	997, 1176, 1486, 1564, 1643, 1939, 2219, 2342, 2431
LweI	GCATC	7 cut(s)	174, 324, 1618, 2566, 2695, 2745, 2782
MaeII	ACGT	2 cut(s)	362, 2683
MaeIII	GTNAC	6 cut(s)	52, 669, 1099, 1372, 2242, 2674
MbiI	CCGCTC	2 cut(s)	204, 2260
MfeI	CAATTG	3 cut(s)	134, 693, 1641
MflI	RGATCY	5 cut(s)	729, 811, 1210, 1330, 2497
MhlI	GDGCHC	7 cut(s)	1542, 1579, 1787, 2253, 2382, 2532, 2641
MlsI	TGGCCA	2 cut(s)	945, 2267
MluI	ACGCGT	1 cut(s)	1114
MluNI	TGGCCA	2 cut(s)	945, 2267
MlyI	GAGTC	7 cut(s)	956, 1042, 1054, 1919, 2027, 2092, 2093
MmeI	TCCRAC	3 cut(s)	910, 1690, 2101
Mox20I	TGGCCA	2 cut(s)	945, 2267
Mph1103I	ATGCAT	2 cut(s)	167, 2710
MroI	TCCGGA	1 cut(s)	455
MroXI	GAANNNNTTC	2 cut(s)	1821, 2195
MscI	TGGCCA	2 cut(s)	945, 2267
MseI	TTAA	5 cut(s)	494, 605, 650, 773, 2949
MslI	CAYNNNNRTG	3 cut(s)	160, 182, 849
Msp20I	TGGCCA	2 cut(s)	945, 2267
MspA1I	CMGCKG	4 cut(s)	1499, 1634, 2333, 2504
MspI	CCGG	2 cut(s)	62, 456
MspR9I	CCNGG	6 cut(s)	18, 99, 1087, 1580, 2303, 2384
MunI	CAATTG	3 cut(s)	134, 693, 1641
Mva1269I	GAATGC	3 cut(s)	167, 1009, 1889
MvaI	CCWGG	6 cut(s)	18, 99, 1087, 1580, 2303, 2384
MvnI	CGCG	1 cut(s)	1116
NcoI	CCATGG	2 cut(s)	2390, 2912
NdeI	CATATG	2 cut(s)	1022, 2239
NheI	GCTAGC	1 cut(s)	2579
NlaIV	GGNNCC	6 cut(s)	22, 1332, 1786, 2379, 2388, 2930
NmeAIII	GCCGAG	1 cut(s)	132
NmuCI	GTSAC	4 cut(s)	52, 669, 1099, 1372
NsbI	TGCGCA	1 cut(s)	1193
NsiI	ATGCAT	2 cut(s)	167, 2710
NspI	RCATGY	4 cut(s)	848, 2367, 2863, 2956
NspV	TTCGAA	1 cut(s)	68
OliI	CACNNNNGTG	1 cut(s)	182
PagI	TCATGA	1 cut(s)	2788
PaqCI	CACCTGC	1 cut(s)	1551
PceI	AGGCCT	2 cut(s)	218, 2158
PciI	ACATGT	1 cut(s)	2952
PctI	GAATGC	3 cut(s)	167, 1009, 1889
PdmI	GAANNNNTTC	2 cut(s)	1821, 2195
PfeI	GAWTC	2 cut(s)	503, 752
PflMI	CCANNNNNTGG	1 cut(s)	2308
PfoI	TCCNGGA	2 cut(s)	16, 97
PleI	GAGTC	7 cut(s)	956, 1041, 1053, 1918, 2026, 2092, 2092
PpsI	GAGTC	7 cut(s)	956, 1041, 1053, 1918, 2026, 2092, 2092
PpuMI	RGGWCCY	2 cut(s)	487, 2386
PscI	ACATGT	1 cut(s)	2952
Psp124BI	GAGCTC	2 cut(s)	2253, 2532
Psp5II	RGGWCCY	2 cut(s)	487, 2386
Psp6I	CCWGG	6 cut(s)	16, 97, 1085, 1578, 2301, 2382
PspEI	GGTNACC	1 cut(s)	2674
PspFI	CCCAGC	2 cut(s)	25, 2333
PspGI	CCWGG	6 cut(s)	16, 97, 1085, 1578, 2301, 2382
PspN4I	GGNNCC	6 cut(s)	22, 1332, 1786, 2379, 2388, 2930
PspPI	GGNCC	6 cut(s)	104, 199, 245, 487, 2386, 2778
PspPPI	RGGWCCY	2 cut(s)	487, 2386
PstI	CTGCAG	2 cut(s)	801, 2277
PstNI	CAGNNNCTG	1 cut(s)	2087
PsuI	RGATCY	5 cut(s)	729, 811, 1210, 1330, 2497
PvuII	CAGCTG	4 cut(s)	1499, 1634, 2333, 2504
RsaI	GTAC	6 cut(s)	365, 547, 703, 1027, 2279, 2714
RsaNI	GTAC	6 cut(s)	364, 546, 702, 1026, 2278, 2713
RseI	CAYNNNNRTG	3 cut(s)	160, 182, 849
SacI	GAGCTC	2 cut(s)	2253, 2532
SaqAI	TTAA	5 cut(s)	494, 605, 650, 773, 2949
Sau96I	GGNCC	6 cut(s)	104, 199, 245, 487, 2386, 2778
SchI	GAGTC	7 cut(s)	956, 1042, 1054, 1919, 2027, 2092, 2093
ScrFI	CCNGG	6 cut(s)	18, 99, 1087, 1580, 2303, 2384
SduI	GDGCHC	7 cut(s)	1542, 1579, 1787, 2253, 2382, 2532, 2641
SfaNI	GCATC	7 cut(s)	174, 324, 1618, 2566, 2695, 2745, 2782
SfcI	CTRYAG	4 cut(s)	797, 955, 1833, 2273
SfuI	TTCGAA	1 cut(s)	68
SinI	GGWCC	5 cut(s)	199, 245, 487, 2386, 2778
SmiMI	CAYNNNNRTG	3 cut(s)	160, 182, 849
SmlI	CTYRAG	6 cut(s)	595, 1030, 1296, 1322, 1535, 1802
SmoI	CTYRAG	6 cut(s)	595, 1030, 1296, 1322, 1535, 1802
SpeI	ACTAGT	1 cut(s)	1144
SseBI	AGGCCT	2 cut(s)	218, 2158
SsiI	CCGC	5 cut(s)	202, 1334, 1528, 2260, 2853
SstI	GAGCTC	2 cut(s)	2253, 2532
StuI	AGGCCT	2 cut(s)	218, 2158
StyD4I	CCNGG	6 cut(s)	16, 97, 1085, 1578, 2301, 2382
StyI	CCWWGG	2 cut(s)	2390, 2912
TaaI	ACNGT	7 cut(s)	7, 268, 550, 669, 910, 1717, 2475
TaiI	ACGT	2 cut(s)	365, 2686
TaqI	TCGA	6 cut(s)	68, 75, 168, 188, 1217, 1573
TaqII	GACCGA	2 cut(s)	233, 2795
TatI	WGTACW	2 cut(s)	701, 2712
TauI	GCSGC	1 cut(s)	2856
TfiI	GAWTC	2 cut(s)	503, 752
Tru1I	TTAA	5 cut(s)	494, 605, 650, 773, 2949
Tru9I	TTAA	5 cut(s)	494, 605, 650, 773, 2949
TseFI	GTSAC	4 cut(s)	52, 669, 1099, 1372
TseI	GCWGC	9 cut(s)	985, 1189, 1499, 1552, 1631, 1952, 2207, 2330, 2419
Tsp45I	GTSAC	4 cut(s)	52, 669, 1099, 1372
TspDTI	ATGAA	8 cut(s)	216, 434, 1578, 1763, 1899, 2040, 2192, 2370
TspGWI	ACGGA	1 cut(s)	698
Van91I	CCANNNNNTGG	1 cut(s)	2308
VpaK11BI	GGWCC	5 cut(s)	199, 245, 487, 2386, 2778
XapI	RAATTY	4 cut(s)	715, 1236, 1966, 2884
XbaI	TCTAGA	1 cut(s)	526
XceI	RCATGY	4 cut(s)	848, 2367, 2863, 2956
XmnI	GAANNNNTTC	2 cut(s)	1821, 2195
Zsp2I	ATGCAT	2 cut(s)	167, 2710

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.