MD14G1246000.v1.1

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009787
Physical Location & Seq
Reverse (-)
32378961 .. 32382924
3964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1246000.v1.1.491

Sequence Viewer

Length: 2202 bp
ATGGGGGGTCTAGACACTCAGAGGCCGTATCAACTTGAAGAAGATGATAACGATCGTCATCATCATAACATAAATTCATCACCATCATCAGGAGATGCGGAGCTGGATTCCCAGCACCAGACTATGACAACGAACAAGAAGAAGAACAAGTACGTGGTGGTGGAAGACGATGACGATGACACGGCAAAGGAAGAATTACTGTCGGTGGAGAAGTTGTTCGAGAACCAACCAGTGCCGTCATGGCGGGAGCAGCTCACGTTCAGAGCCTTCTTCATAAGCTTCGTCCTCAGCATCCTCTTCAGCGTCATCGTCATGAAGCTCAACCTCACCACCGGTATTATCCCCTCTCTCAACGTCTCTGCGGGTCTCTTGGGCTTCTTCTTTGTCAGGACTTGGACCAAGTTCCTCGAGAGGTGCGGCCTCCTCAACCAGCCCTTCACCAGGCAGGAGAACACCGTCATCCAGACCTGCGTCGTTGCCTCCTCCGGGATCGCCTTCAGCGGAGGGTTTGGGAGCTATCTGTTTGGAATGAGCGAACGCATTGCCCAGCAATCCAAAGACAGGAGTGACACAAAAGATCCAGGATTACTCTGGATCATTGGCTTTCTCTTTGTTGTTAGCTTTCTGGGCCTCTTCTCAGTCGTGCCTCTCCGAAAGATCATGATCATAGACTTCAAATTGACATATCCAAGTGGCACTGCAACTGCTTACCTTATCAACAGTTTCCACACTCCCGAAGGAGTCAAGCTAGCCAAGAAACAAGTAAGGGAGTTGGGCAAGTTCTTCTCCTTCAGTTTTTTGTGGGGGTTCTTTCAGTGGTTCTATACTGCCGGAGACAATTGTGGATTTGCAAGCTTCCCTTCACTTGGCCTCAAAGCATATCAGAATAAGTTCTTCTTTGATTTCTCGGCAACATATGTTGGAGTGGGGATGATTTGCCCCTACAATGTGAACATATCAGTTCTTCTTGGAGGCATTCTTTCCTGGGGATTAATGTGGCCTCTCATAGAAACCAGGAGTGGTGATTGGTACGATAAAGAGCTCAGCCCAAAAAGCATGAATGGCCTTCAAGGTTACAAGGTCTTCATTGCCATTGCCCTGATCCTTGGTGATGGGCTATACAACTTCGTGAAGGTGCTAAGTCGAACCCTCATTGCCTTACAATCTCAACTCCGAAGCAAAGATGCGAGCGGCATCCTCCCTGTTAAGAAACATCAAGGCTCTCCAGATGTGAGCTCTGAGCTGTCTTATGATGACCAGCGGCGGACCCAGCTGTTTCTTAAAGACCAAATCCCAGCGTGGATAGCTGTTGGAGGGTATGTTGTGTTTGCTGCAATCTCAATTGCCACTGTGCCGCACATATTTCCCCAACTCAAGTGGTACTATATATTGGTTATCTACCTCTTTGCACCCACGTTGGCTTTCTGTAATGCATACGGTTGTGGCCTCACTGACTGGTCCCTGGCATCCACTTACGGTAAGGTGGCCATCTTCACGATTGGGGCATGGGCCGGTTCTGCACATGGTGGTGTTCTTGCTGGACTAGCAGCATGTGGAGTTATGATGAACATTGTGTCGACAGCCTCTGATCTAACCCAGGATTTCAAGACCGGCTACCTAACCTTGGCTTCCCCTCGGTCCATGTTTGTGAGCCAAGTCGTTGGGACAGCAATGGGTTGTGTAGTTTCTCCTTGTGTGTTTTGGCTCTTCTACAAGGCCTTCAGTGACCTCGGAATACCTGAAAGTGCGTATCCATCTCCCTTTGCTGTTGTGTACCGTAACATAGCTGTCTTGGCGGTAGAAGGTGTAAAATCTCTACCCAAGAACTGCCTCTTGCTTTGTGGCATCTTCTTTGGGGCGGCCATTGCCATAAACGTGCTCAAGGATTGCCTTGGTAAAAAGAGGGGACGCTTTGTTCCACTACCAATGGCAATGGCAATCCCTTTCTATCTAGGGTCGTATTTTGCGATCGACATGTGCATTGGGAGCTTGATAGTGTTTTTGTGGGGAAGGGTAAACAAAGCCAAGGCAGACGCTTTTGTCCCTGCCGTAGCTTCTGGTTTGATTTGCGGGGACGGTACTTGGACTTTGCCTGCTTCAATACTCGCTCTTGCCGGGGTTCAGCCGCCAATTTGCATGAAGTTTTTGTCAAGCTCACAAAACACTAGAGTTGATGCATTCATAGCTGGTAAAGCGCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

734

Amino Acids

80.57

Weight (kDa)

8.7

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 84 - 702 8.7e-169 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2039
Acc36I ACCTGC 1 cut(s) 476
AccBSI CCGCTC 1 cut(s) 1191
AccI GTMKAC 1 cut(s) 1577
AccII CGCG 1 cut(s) 2197
AclWI GGATC 4 cut(s) 497, 572, 602, 1096
AcoI YGGCCR 2 cut(s) 1485, 1860
AcsI RAATTY 1 cut(s) 73
AcuI CTGAAG 4 cut(s) 283, 481, 775, 1705
AdeI CACNNNGTG 1 cut(s) 1526
AfaI GTAC 5 cut(s) 152, 1031, 1382, 1775, 2080
AfiI CCNNNNNNNGG 6 cut(s) 89, 441, 486, 561, 866, 1624
AflIII ACRYGT 1 cut(s) 1974
AgeI ACCGGT 1 cut(s) 332
AgsI TTSAA 5 cut(s) 38, 676, 1070, 1606, 2100
AjnI CCWGG 6 cut(s) 440, 580, 983, 1013, 1461, 1596
AloI GAACNNNNNNTCC 4 cut(s) 200, 232, 1899, 1931
Alw21I GWGCWC 3 cut(s) 1044, 1238, 1881
Alw26I GTCTC 3 cut(s) 361, 371, 828
AlwI GGATC 4 cut(s) 497, 572, 602, 1096
AlwNI CAGNNNCTG 1 cut(s) 1586
Ama87I CYCGRG 1 cut(s) 407
ApeKI GCWGC 3 cut(s) 250, 1331, 1547
ApoI RAATTY 1 cut(s) 73
AseI ATTAAT 1 cut(s) 992
AsiGI ACCGGT 1 cut(s) 332
Asp700I GAANNNNTTC 2 cut(s) 215, 890
AspLEI GCGC 2 cut(s) 2197, 2199
AspS9I GGNCC 6 cut(s) 396, 628, 1266, 1458, 1509, 1638
AsuC2I CCSGG 2 cut(s) 487, 2116
AsuHPI GGTGA 5 cut(s) 72, 319, 430, 1034, 1121
AsuNHI GCTAGC 1 cut(s) 748
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 4 cut(s) 396, 1266, 1458, 1638
BalI TGGCCA 1 cut(s) 1487
BanII GRGCYC 2 cut(s) 1044, 1238
BarI GAAGNNNNNNTAC 2 cut(s) 134, 166
BbsI GAAGAC 2 cut(s) 171, 1075
Bbv12I GWGCWC 3 cut(s) 1044, 1238, 1881
BbvCI CCTCAGC 1 cut(s) 287
BbvI GCAGC 3 cut(s) 262, 1318, 1559
BccI CCATC 4 cut(s) 91, 1106, 1496, 1762
BceAI ACGGC 4 cut(s) 10, 198, 220, 2033
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 6 cut(s) 442, 582, 985, 1015, 1463, 1598
BciVI GTATCC 1 cut(s) 1761
BclI TGATCA 1 cut(s) 663
BcnI CCSGG 2 cut(s) 487, 2116
BcoDI GTCTC 3 cut(s) 361, 371, 828
BfaI CTAG 5 cut(s) 11, 749, 1544, 1952, 2166
BfuAI ACCTGC 1 cut(s) 476
BfuI GTATCC 1 cut(s) 1761
BglI GCCNNNNNGGC 1 cut(s) 241
BisI GCNGC 9 cut(s) 251, 418, 1192, 1262, 1332, 1355, 1548, 1860, 2126
BlpI GCTNAGC 1 cut(s) 1043
BlsI GCNGC 9 cut(s) 252, 419, 1193, 1263, 1333, 1356, 1549, 1861, 2127
Bme1390I CCNGG 8 cut(s) 442, 487, 582, 985, 1015, 1463, 1598, 2116
Bme18I GGWCC 4 cut(s) 396, 1266, 1458, 1638
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 6 cut(s) 396, 628, 1266, 1458, 1509, 1638
BmiI GGNNCC 2 cut(s) 1268, 1460
BmrFI CCNGG 8 cut(s) 442, 487, 582, 985, 1015, 1463, 1598, 2116
BmsI GCATC 7 cut(s) 85, 300, 1174, 1203, 1475, 1854, 2164
BmtI GCTAGC 1 cut(s) 752
BoxI GACNNNNGTC 1 cut(s) 470
BpiI GAAGAC 2 cut(s) 171, 1075
BpmI CTGGAG 1 cut(s) 1209
Bpu10I CCTNAGC 1 cut(s) 287
Bpu1102I GCTNAGC 1 cut(s) 1043
BpuEI CTTGAG 2 cut(s) 1358, 1865
BpuMI CCSGG 2 cut(s) 487, 2116
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 3 cut(s) 51, 57, 662
BsaI GGTCTC 1 cut(s) 371
BsaWI WCCGGW 1 cut(s) 332
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 1305, 1335
Bsc4I CCNNNNNNNGG 6 cut(s) 89, 441, 486, 561, 866, 1624
Bse118I RCCGGY 3 cut(s) 332, 1511, 1610
Bse1I ACTGG 2 cut(s) 230, 1460
Bse3DI GCAATG 7 cut(s) 540, 1086, 1092, 1152, 1677, 1863, 1938
Bse8I GATNNNNATC 3 cut(s) 51, 57, 662
BseBI CCWGG 6 cut(s) 442, 582, 985, 1015, 1463, 1598
BseGI GGATG 5 cut(s) 291, 459, 936, 1194, 1466
BseJI GATNNNNATC 3 cut(s) 51, 57, 662
BseLI CCNNNNNNNGG 6 cut(s) 89, 441, 486, 561, 866, 1624
BseMI GCAATG 7 cut(s) 540, 1086, 1092, 1152, 1677, 1863, 1938
BseMII CTCAG 5 cut(s) 32, 301, 651, 1057, 1230
BseNI ACTGG 2 cut(s) 230, 1460
BsePI GCGCGC 1 cut(s) 2195
BseRI GAGGAG 2 cut(s) 413, 472
BseXI GCAGC 3 cut(s) 262, 1318, 1559
BseYI CCCAGC 4 cut(s) 111, 546, 1269, 1294
BsgI GTGCAG 1 cut(s) 1503
Bsh1236I CGCG 1 cut(s) 2197
Bsh1285I CGRYCG 2 cut(s) 55, 1971
BshTI ACCGGT 1 cut(s) 332
BsiEI CGRYCG 2 cut(s) 55, 1971
BsiHKAI GWGCWC 3 cut(s) 1044, 1238, 1881
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 6 cut(s) 333, 486, 831, 1512, 1611, 2115
BslFI GGGAC 5 cut(s) 1444, 1678, 1920, 2027, 2087
BslI CCNNNNNNNGG 6 cut(s) 89, 441, 486, 561, 866, 1624
BsmAI GTCTC 3 cut(s) 361, 371, 828
BsmBI CGTCTC 1 cut(s) 361
BsmFI GGGAC 5 cut(s) 1444, 1678, 1920, 2027, 2087
BsmI GAATGC 2 cut(s) 975, 2177
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 3 cut(s) 1044, 1238, 1881
Bsp143I GATC 9 cut(s) 52, 489, 577, 594, 657, 663, 1101, 1588, 1968
Bsp1720I GCTNAGC 1 cut(s) 1043
BspCNI CTCAG 5 cut(s) 31, 300, 650, 1056, 1231
BspFNI CGCG 1 cut(s) 2197
BspHI TCATGA 2 cut(s) 312, 660
BspLI GGNNCC 2 cut(s) 1268, 1460
BspMI ACCTGC 1 cut(s) 476
BspOI GCTAGC 1 cut(s) 752
BspPI GGATC 4 cut(s) 497, 572, 602, 1096
BspQI GCTCTTC 1 cut(s) 1712
BspTNI GGTCTC 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 1191
BsrDI GCAATG 7 cut(s) 540, 1086, 1092, 1152, 1677, 1863, 1938
BsrFI RCCGGY 3 cut(s) 332, 1511, 1610
BsrI ACTGG 2 cut(s) 230, 1460
BssAI RCCGGY 3 cut(s) 332, 1511, 1610
BssHII GCGCGC 1 cut(s) 2195
BssMI GATC 9 cut(s) 52, 489, 577, 594, 657, 663, 1101, 1588, 1968
BssT1I CCWWGG 4 cut(s) 1105, 1623, 1891, 2025
Bst2UI CCWGG 6 cut(s) 442, 582, 985, 1015, 1463, 1598
Bst4CI ACNGT 8 cut(s) 201, 457, 722, 1351, 1439, 1478, 1778, 2078
Bst6I CTCTTC 3 cut(s) 302, 638, 1712
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 5 cut(s) 750, 853, 1189, 2094, 2197
BstDEI CTNAG 6 cut(s) 18, 287, 637, 1043, 1139, 1239
BstENI CCTNNNNNAGG 1 cut(s) 439
BstF5I GGATG 5 cut(s) 291, 459, 936, 1194, 1466
BstFNI CGCG 1 cut(s) 2197
BstHHI GCGC 2 cut(s) 2197, 2199
BstKTI GATC 9 cut(s) 55, 492, 580, 597, 660, 666, 1104, 1591, 1971
BstMAI GTCTC 3 cut(s) 361, 371, 828
BstMBI GATC 9 cut(s) 52, 489, 577, 594, 657, 663, 1101, 1588, 1968
BstMCI CGRYCG 2 cut(s) 55, 1971
BstNI CCWGG 6 cut(s) 442, 582, 985, 1015, 1463, 1598
BstNSI RCATGY 2 cut(s) 1554, 1978
BstPAI GACNNNNGTC 1 cut(s) 470
BstSCI CCNGG 8 cut(s) 440, 485, 580, 983, 1013, 1461, 1596, 2114
BstUI CGCG 1 cut(s) 2197
BstV1I GCAGC 3 cut(s) 262, 1318, 1559
BstV2I GAAGAC 2 cut(s) 171, 1075
BstX2I RGATCY 1 cut(s) 577
BstXI CCANNNNNNTGG 1 cut(s) 1661
BstYI RGATCY 1 cut(s) 577
BsuI GTATCC 1 cut(s) 1761
BtsCI GGATG 5 cut(s) 291, 459, 936, 1194, 1466
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 6 cut(s) 237, 696, 821, 1347, 1449, 1729
BveI ACCTGC 1 cut(s) 476
Cac8I GCNNGC 5 cut(s) 750, 853, 1189, 2094, 2197
CaiI CAGNNNCTG 1 cut(s) 1586
CciI TCATGA 2 cut(s) 312, 660
CfoI GCGC 2 cut(s) 2197, 2199
Cfr10I RCCGGY 3 cut(s) 332, 1511, 1610
Cfr13I GGNCC 6 cut(s) 396, 628, 1266, 1458, 1509, 1638
CseI GACGC 4 cut(s) 292, 460, 1917, 2042
Csp6I GTAC 5 cut(s) 151, 1030, 1381, 1774, 2079
CspAI ACCGGT 1 cut(s) 332
CspCI CAANNNNNGTGG 2 cut(s) 1358, 1393
CviQI GTAC 5 cut(s) 151, 1030, 1381, 1774, 2079
DdeI CTNAG 6 cut(s) 18, 287, 637, 1043, 1139, 1239
DpnI GATC 9 cut(s) 54, 491, 579, 596, 659, 665, 1103, 1590, 1970
DpnII GATC 9 cut(s) 52, 489, 577, 594, 657, 663, 1101, 1588, 1968
DraIII CACNNNGTG 1 cut(s) 1526
DrdI GACNNNNNNGTC 1 cut(s) 2039
DseDI GACNNNNNNGTC 1 cut(s) 2039
EaeI YGGCCR 2 cut(s) 1485, 1860
Eam1104I CTCTTC 3 cut(s) 302, 638, 1712
EarI CTCTTC 3 cut(s) 302, 638, 1712
EciI GGCGGA 1 cut(s) 1279
Ecl136II GAGCTC 2 cut(s) 1042, 1236
Eco130I CCWWGG 4 cut(s) 1105, 1623, 1891, 2025
Eco147I AGGCCT 1 cut(s) 1718
Eco24I GRGCYC 2 cut(s) 1044, 1238
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 4 cut(s) 396, 1266, 1458, 1638
Eco53kI GAGCTC 2 cut(s) 1042, 1236
Eco57I CTGAAG 4 cut(s) 283, 481, 775, 1705
Eco88I CYCGRG 1 cut(s) 407
EcoICRI GAGCTC 2 cut(s) 1042, 1236
EcoNI CCTNNNNNAGG 1 cut(s) 439
EcoRII CCWGG 6 cut(s) 440, 580, 983, 1013, 1461, 1596
EcoT14I CCWWGG 4 cut(s) 1105, 1623, 1891, 2025
EcoT22I ATGCAT 2 cut(s) 1435, 2179
EcoT38I GRGCYC 2 cut(s) 1044, 1238
ErhI CCWWGG 4 cut(s) 1105, 1623, 1891, 2025
Esp3I CGTCTC 1 cut(s) 361
FalI AAGNNNNNCTT 4 cut(s) 844, 876, 881, 913
FaqI GGGAC 5 cut(s) 1444, 1678, 1920, 2027, 2087
FauI CCCGC 3 cut(s) 237, 355, 2063
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 1 cut(s) 663
FblI GTMKAC 1 cut(s) 1577
Fnu4HI GCNGC 9 cut(s) 251, 418, 1192, 1262, 1332, 1355, 1548, 1860, 2126
FokI GGATG 5 cut(s) 278, 446, 943, 1181, 1453
FriOI GRGCYC 2 cut(s) 1044, 1238
Fsp4HI GCNGC 9 cut(s) 251, 418, 1192, 1262, 1332, 1355, 1548, 1860, 2126
FspBI CTAG 5 cut(s) 11, 749, 1544, 1952, 2166
GlaI GCGC 2 cut(s) 2196, 2198
GluI GCNGC 9 cut(s) 251, 418, 1192, 1262, 1332, 1355, 1548, 1860, 2126
GsaI CCCAGC 4 cut(s) 115, 550, 1273, 1298
GsuI CTGGAG 1 cut(s) 1209
HapII CCGG 6 cut(s) 333, 486, 831, 1512, 1611, 2115
HgaI GACGC 4 cut(s) 292, 460, 1917, 2042
HhaI GCGC 2 cut(s) 2197, 2199
Hin6I GCGC 2 cut(s) 2195, 2197
HinP1I GCGC 2 cut(s) 2195, 2197
HincII GTYRAC 1 cut(s) 1578
HindII GTYRAC 1 cut(s) 1578
HindIII AAGCTT 2 cut(s) 277, 853
HinfI GANTC 2 cut(s) 107, 741
HpaII CCGG 6 cut(s) 333, 486, 831, 1512, 1611, 2115
HphI GGTGA 5 cut(s) 72, 319, 430, 1034, 1121
Hpy166II GTNNAC 4 cut(s) 952, 1578, 1774, 2017
Hpy188I TCNGA 8 cut(s) 21, 263, 653, 885, 1175, 1240, 1588, 1733
Hpy8I GTNNAC 4 cut(s) 952, 1578, 1774, 2017
Hpy99I CGWCG 1 cut(s) 476
HpyCH4III ACNGT 8 cut(s) 201, 457, 722, 1351, 1439, 1478, 1778, 2078
HpyCH4IV ACGT 5 cut(s) 153, 257, 354, 1415, 1875
HpyCH4V TGCA 9 cut(s) 701, 851, 1334, 1409, 1433, 1520, 1980, 2136, 2177
HpyF3I CTNAG 6 cut(s) 18, 287, 637, 1043, 1139, 1239
HpySE526I ACGT 5 cut(s) 153, 257, 354, 1415, 1875
HspAI GCGC 2 cut(s) 2195, 2197
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 9 cut(s) 52, 489, 577, 594, 657, 663, 1101, 1588, 1968
LguI GCTCTTC 1 cut(s) 1712
LmnI GCTCC 4 cut(s) 100, 247, 513, 1986
Lsp1109I GCAGC 3 cut(s) 262, 1318, 1559
LweI GCATC 7 cut(s) 85, 300, 1174, 1203, 1475, 1854, 2164
MaeI CTAG 5 cut(s) 11, 749, 1544, 1952, 2166
MaeII ACGT 5 cut(s) 153, 257, 354, 1415, 1875
MaeIII GTNAC 4 cut(s) 566, 1073, 1724, 1778
MalI GATC 9 cut(s) 54, 491, 579, 596, 659, 665, 1103, 1590, 1970
MbiI CCGCTC 1 cut(s) 1191
MboI GATC 9 cut(s) 52, 489, 577, 594, 657, 663, 1101, 1588, 1968
MfeI CAATTG 2 cut(s) 838, 1341
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 3 cut(s) 1044, 1238, 1881
MlsI TGGCCA 1 cut(s) 1487
MluCI AATT 6 cut(s) 73, 194, 677, 838, 1341, 2130
MluNI TGGCCA 1 cut(s) 1487
MlyI GAGTC 1 cut(s) 750
MmeI TCCRAC 2 cut(s) 901, 1291
Mox20I TGGCCA 1 cut(s) 1487
Mph1103I ATGCAT 2 cut(s) 1435, 2179
MroXI GAANNNNTTC 2 cut(s) 215, 890
MscI TGGCCA 1 cut(s) 1487
MseI TTAA 3 cut(s) 992, 1206, 1281
MslI CAYNNNNRTG 4 cut(s) 311, 1527, 1646, 1874
Msp20I TGGCCA 1 cut(s) 1487
MspA1I CMGCKG 3 cut(s) 501, 1261, 1273
MspI CCGG 6 cut(s) 333, 486, 831, 1512, 1611, 2115
MspR9I CCNGG 8 cut(s) 442, 487, 582, 985, 1015, 1463, 1598, 2116
MunI CAATTG 2 cut(s) 838, 1341
Mva1269I GAATGC 2 cut(s) 975, 2177
MvaI CCWGG 6 cut(s) 442, 582, 985, 1015, 1463, 1598
MvnI CGCG 1 cut(s) 2197
NciI CCSGG 2 cut(s) 487, 2116
NdeI CATATG 1 cut(s) 916
NdeII GATC 9 cut(s) 52, 489, 577, 594, 657, 663, 1101, 1588, 1968
NheI GCTAGC 1 cut(s) 748
NlaIV GGNNCC 2 cut(s) 1268, 1460
NmeAIII GCCGAG 1 cut(s) 887
NmuCI GTSAC 2 cut(s) 566, 1724
NsiI ATGCAT 2 cut(s) 1435, 2179
NspI RCATGY 2 cut(s) 1554, 1978
PaeR7I CTCGAG 1 cut(s) 407
PagI TCATGA 2 cut(s) 312, 660
PauI GCGCGC 1 cut(s) 2195
PceI AGGCCT 1 cut(s) 1718
PciI ACATGT 1 cut(s) 1974
PciSI GCTCTTC 1 cut(s) 1712
PcsI WCGNNNNNNNCGW 1 cut(s) 1964
PctI GAATGC 2 cut(s) 975, 2177
PdmI GAANNNNTTC 2 cut(s) 215, 890
PfeI GAWTC 1 cut(s) 107
PfoI TCCNGGA 2 cut(s) 485, 580
PinAI ACCGGT 1 cut(s) 332
PkrI GCNGC 9 cut(s) 252, 419, 1193, 1263, 1333, 1356, 1549, 1861, 2127
Ple19I CGATCG 2 cut(s) 55, 1971
PleI GAGTC 1 cut(s) 749
PpsI GAGTC 1 cut(s) 749
Ppu21I YACGTR 1 cut(s) 154
PscI ACATGT 1 cut(s) 1974
PshAI GACNNNNGTC 1 cut(s) 470
PshBI ATTAAT 1 cut(s) 992
Psp124BI GAGCTC 2 cut(s) 1044, 1238
Psp6I CCWGG 6 cut(s) 440, 580, 983, 1013, 1461, 1596
PspFI CCCAGC 4 cut(s) 111, 546, 1269, 1294
PspGI CCWGG 6 cut(s) 440, 580, 983, 1013, 1461, 1596
PspN4I GGNNCC 2 cut(s) 1268, 1460
PspPI GGNCC 6 cut(s) 396, 628, 1266, 1458, 1509, 1638
PstNI CAGNNNCTG 1 cut(s) 1586
PsuI RGATCY 1 cut(s) 577
PteI GCGCGC 1 cut(s) 2195
PvuI CGATCG 2 cut(s) 55, 1971
PvuII CAGCTG 1 cut(s) 1273
RsaI GTAC 5 cut(s) 152, 1031, 1382, 1775, 2080
RsaNI GTAC 5 cut(s) 151, 1030, 1381, 1774, 2079
RseI CAYNNNNRTG 4 cut(s) 311, 1527, 1646, 1874
SacI GAGCTC 2 cut(s) 1044, 1238
SalI GTCGAC 1 cut(s) 1576
SapI GCTCTTC 1 cut(s) 1712
SaqAI TTAA 3 cut(s) 992, 1206, 1281
SatI GCNGC 9 cut(s) 251, 418, 1192, 1262, 1332, 1355, 1548, 1860, 2126
Sau3AI GATC 9 cut(s) 52, 489, 577, 594, 657, 663, 1101, 1588, 1968
Sau96I GGNCC 6 cut(s) 396, 628, 1266, 1458, 1509, 1638
SchI GAGTC 1 cut(s) 750
ScrFI CCNGG 8 cut(s) 442, 487, 582, 985, 1015, 1463, 1598, 2116
SduI GDGCHC 3 cut(s) 1044, 1238, 1881
SfaNI GCATC 7 cut(s) 85, 300, 1174, 1203, 1475, 1854, 2164
Sfr274I CTCGAG 1 cut(s) 407
SinI GGWCC 4 cut(s) 396, 1266, 1458, 1638
SlaI CTCGAG 1 cut(s) 407
SmiMI CAYNNNNRTG 4 cut(s) 311, 1527, 1646, 1874
SmlI CTYRAG 3 cut(s) 407, 1373, 1880
SmoI CTYRAG 3 cut(s) 407, 1373, 1880
Sse9I AATT 6 cut(s) 73, 194, 677, 838, 1341, 2130
SseBI AGGCCT 1 cut(s) 1718
SspMI CTAG 5 cut(s) 11, 749, 1544, 1952, 2166
SstI GAGCTC 2 cut(s) 1044, 1238
StuI AGGCCT 1 cut(s) 1718
StyD4I CCNGG 8 cut(s) 440, 485, 580, 983, 1013, 1461, 1596, 2114
StyI CCWWGG 4 cut(s) 1105, 1623, 1891, 2025
TaaI ACNGT 8 cut(s) 201, 457, 722, 1351, 1439, 1478, 1778, 2078
TaiI ACGT 5 cut(s) 156, 260, 357, 1418, 1878
TaqI TCGA 5 cut(s) 219, 408, 1144, 1577, 1971
TaqII GACCGA 1 cut(s) 1626
TasI AATT 6 cut(s) 73, 194, 677, 838, 1341, 2130
TauI GCSGC 6 cut(s) 420, 1194, 1264, 1357, 1862, 2128
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 3 cut(s) 992, 1206, 1281
Tru9I TTAA 3 cut(s) 992, 1206, 1281
TscAI CASTG 6 cut(s) 237, 703, 821, 1354, 1456, 1729
TseFI GTSAC 2 cut(s) 566, 1724
TseI GCWGC 3 cut(s) 250, 1331, 1547
Tsp45I GTSAC 2 cut(s) 566, 1724
TspDTI ATGAA 8 cut(s) 66, 262, 329, 1073, 1075, 1580, 2153, 2170
TspRI CASTG 6 cut(s) 237, 703, 821, 1354, 1456, 1729
VpaK11BI GGWCC 4 cut(s) 396, 1266, 1458, 1638
VspI ATTAAT 1 cut(s) 992
XagI CCTNNNNNAGG 1 cut(s) 439
XapI RAATTY 1 cut(s) 73
XbaI TCTAGA 1 cut(s) 10
XceI RCATGY 2 cut(s) 1554, 1978
XcmI CCANNNNNNNNNTGG 2 cut(s) 237, 588
XhoI CTCGAG 1 cut(s) 407
XmiI GTMKAC 1 cut(s) 1577
XmnI GAANNNNTTC 2 cut(s) 215, 890
XspI CTAG 5 cut(s) 11, 749, 1544, 1952, 2166
Zsp2I ATGCAT 2 cut(s) 1435, 2179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.