MD14G1247500.v1.1

Sm-like protein LSM8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011059
Physical Location & Seq
Reverse (-)
32499343 .. 32501394
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1247500.v1.1.491

Sequence Viewer

Length: 297 bp
ATGTCGAGTGGCACTGGTCTTGAGTCACTCGTTGATCAAACAATTTCAGTTATCACAAATGATGGACGGAATATTGTGGGAGTCTTGAAAGGCTTTGACCAGTCTACTAATATCATTCTCGATGAATCTCATGAACGTGTTTTCTCTACAAAGGAGGGTGTTCAGCAACTCGTGTTGGGGTTATATATAATCAGAGGTGACAACATAAGCATTGTTGGGGAATTAGATGCAGATCTTGATTCTACTGTTGAGTGGTCAAAGATGAGAGCTTATCCCCTCAAGCCTGTCATCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 ko03040 map03018 map03040
Pfam Domains
Protein Families
LSM

Protein Analysis

99

Amino Acids

10.75

Weight (kDa)

4.64

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 7 - 73 2.6e-18 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 104
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 1 cut(s) 88
AluBI AGCT 1 cut(s) 269
AluI AGCT 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 209
BauI CACGAG 1 cut(s) 170
BccI CCATC 1 cut(s) 56
BclI TGATCA 1 cut(s) 34
BglII AGATCT 1 cut(s) 232
BmsI GCATC 1 cut(s) 217
BpuEI CTTGAG 2 cut(s) 41, 263
BsaBI GATNNNNATC 1 cut(s) 231
Bse1I ACTGG 2 cut(s) 19, 100
Bse8I GATNNNNATC 1 cut(s) 231
BseGI GGATG 1 cut(s) 288
BseJI GATNNNNATC 1 cut(s) 231
BseNI ACTGG 2 cut(s) 19, 100
Bsp143I GATC 2 cut(s) 34, 232
BspHI TCATGA 1 cut(s) 130
BsrI ACTGG 2 cut(s) 19, 100
BssMI GATC 2 cut(s) 34, 232
BssSI CACGAG 1 cut(s) 170
Bst2BI CACGAG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 247
BstF5I GGATG 1 cut(s) 288
BstKTI GATC 2 cut(s) 37, 235
BstMBI GATC 2 cut(s) 34, 232
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BtsCI GGATG 1 cut(s) 288
BtsIMutI CAGTG 2 cut(s) 12, 292
CciI TCATGA 1 cut(s) 130
CviAII CATG 1 cut(s) 131
CviJI RGCY 3 cut(s) 93, 269, 283
CviKI_1 RGCY 3 cut(s) 93, 269, 283
DpnI GATC 2 cut(s) 36, 234
DpnII GATC 2 cut(s) 34, 232
FaeI CATG 1 cut(s) 134
FaiI YATR 5 cut(s) 132, 184, 186, 188, 206
FatI CATG 1 cut(s) 130
FbaI TGATCA 1 cut(s) 34
FblI GTMKAC 1 cut(s) 104
FokI GGATG 1 cut(s) 275
Hin1II CATG 1 cut(s) 134
HinfI GANTC 4 cut(s) 23, 81, 125, 239
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 5 cut(s) 20, 85, 119, 131, 236
Hpy8I GTNNAC 1 cut(s) 105
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 1 cut(s) 230
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 2 cut(s) 34, 232
LpnPI CCDG 1 cut(s) 113
LweI GCATC 1 cut(s) 217
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 2 cut(s) 24, 197
MalI GATC 2 cut(s) 36, 234
MboI GATC 2 cut(s) 34, 232
MflI RGATCY 1 cut(s) 232
MluCI AATT 2 cut(s) 42, 221
MlyI GAGTC 2 cut(s) 32, 90
MnlI CCTC 3 cut(s) 148, 188, 287
MslI CAYNNNNRTG 1 cut(s) 135
NdeII GATC 2 cut(s) 34, 232
NlaIII CATG 1 cut(s) 134
NmuCI GTSAC 2 cut(s) 24, 197
PagI TCATGA 1 cut(s) 130
PfeI GAWTC 2 cut(s) 125, 239
PleI GAGTC 2 cut(s) 31, 89
PpsI GAGTC 2 cut(s) 31, 89
PsuI RGATCY 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 135
Sau3AI GATC 2 cut(s) 34, 232
SchI GAGTC 2 cut(s) 32, 90
SetI ASST 3 cut(s) 139, 199, 271
SfaNI GCATC 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 2 cut(s) 20, 278
SmoI CTYRAG 2 cut(s) 20, 278
Sse9I AATT 2 cut(s) 42, 221
SspI AATATT 1 cut(s) 73
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 139
TaqI TCGA 2 cut(s) 5, 120
TasI AATT 2 cut(s) 42, 221
TfiI GAWTC 2 cut(s) 125, 239
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 2 cut(s) 24, 197
Tsp45I GTSAC 2 cut(s) 24, 197
TspDTI ATGAA 2 cut(s) 138, 147
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.