RchiOBHm_Chr1g0326651

Sm-like protein LSM8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0011059
Physical Location & Seq
Reverse (-)
15176563 .. 15179919
3357 bp
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UTR
Exon/CDS
Intron
PRQ55625

Sequence Viewer

Length: 297 bp
ATGTCCGGTGGCCCTGGTCTTGAGTCACTTGTTGATCAAACAATTTCAGTTATCACAAATGATGGACGCAATATTGTGGGAGTCTTGAAAGGCTTTGACCAGGCTACTAATATCATTCTTGATGAGTCTCATGAACGTGTCTTCTCTACAAAGGAGGGTGTTCAGCAACTTGTGTTGGGATTGTATATAATCAGGGGCGACAACATAAGCATCGTTGGGGAACTAGATGCAGATCTTGATTCAAGCCTTGACTGGTCGAAGATGAGAGCGTATCCTCTGAAGCCTGTCATCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 ko03040 map03018 map03040
Pfam Domains
Protein Families
LSM

Protein Analysis

98

Amino Acids

10.68

Weight (kDa)

4.61

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 7 - 73 1.2e-18 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 2 cut(s) 88, 243
AjnI CCWGG 2 cut(s) 13, 99
Alw26I GTCTC 1 cut(s) 132
AoxI GGCC 1 cut(s) 10
AspS9I GGNCC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 133
BccI CCATC 1 cut(s) 56
BciT130I CCWGG 2 cut(s) 15, 101
BciVI GTATCC 1 cut(s) 282
BclI TGATCA 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 1 cut(s) 224
BfuI GTATCC 1 cut(s) 282
BglII AGATCT 1 cut(s) 232
Bme1390I CCNGG 2 cut(s) 15, 101
BmgT120I GGNCC 1 cut(s) 11
BmrFI CCNGG 2 cut(s) 15, 101
BmsI GCATC 2 cut(s) 217, 219
BpiI GAAGAC 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 13
BsaWI WCCGGW 1 cut(s) 5
Bse1I ACTGG 1 cut(s) 257
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 2 cut(s) 15, 101
BseDI CCNNGG 1 cut(s) 13
BseGI GGATG 1 cut(s) 288
BseJI GATNNNNATC 1 cut(s) 231
BseNI ACTGG 1 cut(s) 257
BshFI GGCC 1 cut(s) 12
BsiSI CCGG 1 cut(s) 6
BsmAI GTCTC 1 cut(s) 132
BsnI GGCC 1 cut(s) 12
Bsp143I GATC 2 cut(s) 34, 232
BspANI GGCC 1 cut(s) 12
BspHI TCATGA 1 cut(s) 130
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 13
BssMI GATC 2 cut(s) 34, 232
Bst2UI CCWGG 2 cut(s) 15, 101
BstF5I GGATG 1 cut(s) 288
BstKTI GATC 2 cut(s) 37, 235
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 34, 232
BstNI CCWGG 2 cut(s) 15, 101
BstSCI CCNGG 2 cut(s) 13, 99
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BsuI GTATCC 1 cut(s) 282
BsuRI GGCC 1 cut(s) 12
BtsCI GGATG 1 cut(s) 288
CciI TCATGA 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 11
CseI GACGC 1 cut(s) 75
CviAII CATG 1 cut(s) 131
CviJI RGCY 5 cut(s) 12, 93, 104, 246, 283
CviKI_1 RGCY 5 cut(s) 12, 93, 104, 246, 283
DpnI GATC 2 cut(s) 36, 234
DpnII GATC 2 cut(s) 34, 232
EcoRII CCWGG 2 cut(s) 13, 99
FaeI CATG 1 cut(s) 134
FaiI YATR 4 cut(s) 132, 186, 188, 206
FatI CATG 1 cut(s) 130
FbaI TGATCA 1 cut(s) 34
FokI GGATG 1 cut(s) 275
FspBI CTAG 1 cut(s) 224
HaeIII GGCC 1 cut(s) 12
HapII CCGG 1 cut(s) 6
HgaI GACGC 1 cut(s) 75
Hin1II CATG 1 cut(s) 134
HinfI GANTC 4 cut(s) 23, 81, 125, 239
HpaII CCGG 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 279
Hpy188III TCNNGA 5 cut(s) 20, 85, 119, 131, 236
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 1 cut(s) 230
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 2 cut(s) 34, 232
LpnPI CCDG 6 cut(s) 19, 27, 86, 113, 178, 238
LweI GCATC 2 cut(s) 217, 219
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 24
MalI GATC 2 cut(s) 36, 234
MboI GATC 2 cut(s) 34, 232
MboII GAAGA 2 cut(s) 133, 271
MflI RGATCY 1 cut(s) 232
MluCI AATT 1 cut(s) 42
MlyI GAGTC 3 cut(s) 32, 90, 134
MnlI CCTC 2 cut(s) 148, 285
MslI CAYNNNNRTG 1 cut(s) 135
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 2 cut(s) 15, 101
MvaI CCWGG 2 cut(s) 15, 101
NdeII GATC 2 cut(s) 34, 232
NlaIII CATG 1 cut(s) 134
NmuCI GTSAC 1 cut(s) 24
PagI TCATGA 1 cut(s) 130
PfeI GAWTC 1 cut(s) 239
PleI GAGTC 3 cut(s) 31, 89, 133
PpsI GAGTC 3 cut(s) 31, 89, 133
Psp6I CCWGG 2 cut(s) 13, 99
PspGI CCWGG 2 cut(s) 13, 99
PspPI GGNCC 1 cut(s) 11
PsuI RGATCY 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 135
Sau3AI GATC 2 cut(s) 34, 232
Sau96I GGNCC 1 cut(s) 11
SchI GAGTC 3 cut(s) 32, 90, 134
ScrFI CCNGG 2 cut(s) 15, 101
SetI ASST 1 cut(s) 139
SfaNI GCATC 2 cut(s) 217, 219
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 1 cut(s) 42
SspI AATATT 1 cut(s) 73
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 2 cut(s) 13, 99
TaiI ACGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 257
TasI AATT 1 cut(s) 42
TfiI GAWTC 1 cut(s) 239
TseFI GTSAC 1 cut(s) 24
Tsp45I GTSAC 1 cut(s) 24
TspDTI ATGAA 1 cut(s) 147
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.