MD15G1049800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012130
Physical Location & Seq
Forward (+)
3393376 .. 3395831
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1049800.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGCCACGCTGAGCAGCACCGCGCACGTTAGCACCAACCCCTTCAGCTCCTCGATTGTCCCTCTCTCACAGCACGTAAAGGTCGCCGCAGCATCAAAGAAGTCACGGCGGGTGAGGTGCGCATTGCCACCCTCGAATTGGCGGGAGAGCAGGCGATTGGTCTCCATCTCTCTGTCACTCGCCCTCTCTAATCTGCTCCTTGTCCCTCACCATGCTGTTGCCGGCGGATTCGACAAGTATGTGAAAAGGAAGAAGCTCGACCCTCTCGAGGCTTATGTGTCGCCAGTCATACTCACCCAATTACAAATCAAGGACTTGGAGAAATCTCTGGAAGGTGATCAACCCCAGTTTGCTACTTGCCGGTCTCTGCTGCGTTCAGGCCCCGCAGCGTCCCTTCGCATAAATATTCGAGCTGTGGCGCAATATGCTTCAGATGATGGTAATGGGAAGGCTGCTTCGAGCGATGTTGATCAATGTCTCAGGGCATTGGAGGAACTGGATTCCTTGCTTTTGCACGCAACAAGGAACGAACGAGCTCCAGTCAGTTCGATGAAGGCACAGATTAATACTGCCCTTAATGCGTTGGACAGCCTCCTCAAAACTGTACCTTCGGAAGTGCTAGACAAGGGGAAGGTAGCGGCCGATTCTTACCGGAACTCGTTAGAGAACGCAGATCTTGATATTTCGGACCCGGACCTGAAGCAGTTGGAATCGATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

25.99

Weight (kDa)

8.96

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7880 PF25306 88 - 211 1.3e-54 Domain of unknown function (DUF7880)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012130)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26340 AT2G26340
fragaria_vesca FvH4_2g30980 FvH4_2g30980 FvH4_2g30980 FvH4_2g30980
malus_domestica MD08G1071400.v1.1 MD15G1049800.v1.1
prunus_persica Prupe.1G404100_v2.0.a1
pyrus_communis pycom08g05930
rosa_chinensis RchiOBHm_Chr6g0311811
rosa_laevigata RLG00000010372
rosa_multiflora Rmu_sc0002888.1_g000044
rosa_roxburghii Rroxscaffold_7G00157290
rosa_samantha Rh6AG507200 Rh6BG518200 Rh6CG523700 Rh6DG509500
rosa_wichuraiana Rw6G044130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 121
AccII CGCG 1 cut(s) 23
AciI CCGC 7 cut(s) 21, 87, 109, 142, 225, 384, 638
AcoI YGGCCR 2 cut(s) 3, 639
AcuI CTGAAG 3 cut(s) 28, 414, 719
AfaI GTAC 1 cut(s) 606
AfiI CCNNNNNNNGG 2 cut(s) 138, 268
AluBI AGCT 4 cut(s) 48, 256, 413, 536
AluI AGCT 4 cut(s) 48, 256, 413, 536
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 3 cut(s) 166, 369, 482
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 3 cut(s) 3, 379, 639
ApeKI GCWGC 5 cut(s) 15, 89, 370, 386, 452
AseI ATTAAT 1 cut(s) 564
AspLEI GCGC 3 cut(s) 25, 122, 421
AspS9I GGNCC 3 cut(s) 380, 688, 694
AsuC2I CCSGG 1 cut(s) 692
AsuHPI GGTGA 4 cut(s) 124, 200, 286, 347
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 2 cut(s) 688, 694
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 538
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 5 cut(s) 27, 101, 357, 398, 439
BccI CCATC 2 cut(s) 173, 431
BceAI ACGGC 1 cut(s) 122
BclI TGATCA 2 cut(s) 337, 469
BcnI CCSGG 1 cut(s) 692
BcoDI GTCTC 3 cut(s) 166, 369, 482
BfaI CTAG 1 cut(s) 620
BglII AGATCT 1 cut(s) 673
BisI GCNGC 7 cut(s) 16, 87, 90, 371, 387, 453, 639
BlpI GCTNAGC 1 cut(s) 11
BlsI GCNGC 7 cut(s) 17, 88, 91, 372, 388, 454, 640
Bme1390I CCNGG 1 cut(s) 692
Bme18I GGWCC 2 cut(s) 688, 694
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 3 cut(s) 380, 688, 694
BmiI GGNNCC 2 cut(s) 382, 690
BmrFI CCNGG 1 cut(s) 692
BmrI ACTGGG 1 cut(s) 340
BmsI GCATC 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 340
BpmI CTGGAG 1 cut(s) 522
Bpu1102I GCTNAGC 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 692
Bsa29I ATCGAT 1 cut(s) 713
BsaAI YACGTR 1 cut(s) 76
BsaBI GATNNNNATC 1 cut(s) 468
BsaI GGTCTC 2 cut(s) 166, 369
BsaWI WCCGGW 1 cut(s) 651
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 268
Bse118I RCCGGY 2 cut(s) 221, 360
Bse1I ACTGG 4 cut(s) 284, 346, 501, 539
Bse3DI GCAATG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 468
BseCI ATCGAT 1 cut(s) 713
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 2 cut(s) 138, 268
BseMI GCAATG 1 cut(s) 122
BseMII CTCAG 1 cut(s) 493
BseNI ACTGG 4 cut(s) 284, 346, 501, 539
BseRI GAGGAG 2 cut(s) 40, 584
BseX3I CGGCCG 1 cut(s) 639
BseXI GCAGC 5 cut(s) 27, 101, 357, 398, 439
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 642
BshFI GGCC 3 cut(s) 5, 381, 641
BshVI ATCGAT 1 cut(s) 713
BsiEI CGRYCG 1 cut(s) 642
BsiHKAI GWGCWC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 4 cut(s) 222, 361, 652, 692
BslFI GGGAC 3 cut(s) 44, 188, 376
BslI CCNNNNNNNGG 2 cut(s) 138, 268
BsmAI GTCTC 3 cut(s) 166, 369, 482
BsmFI GGGAC 3 cut(s) 44, 188, 376
BsnI GGCC 3 cut(s) 5, 381, 641
Bso31I GGTCTC 2 cut(s) 166, 369
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 3 cut(s) 337, 469, 673
Bsp1720I GCTNAGC 1 cut(s) 11
BspACI CCGC 7 cut(s) 21, 87, 109, 142, 225, 384, 638
BspANI GGCC 3 cut(s) 5, 381, 641
BspCNI CTCAG 1 cut(s) 492
BspDI ATCGAT 1 cut(s) 713
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 382, 690
BspTNI GGTCTC 2 cut(s) 166, 369
BsrDI GCAATG 1 cut(s) 122
BsrFI RCCGGY 2 cut(s) 221, 360
BsrI ACTGG 4 cut(s) 284, 346, 501, 539
BssAI RCCGGY 2 cut(s) 221, 360
BssMI GATC 3 cut(s) 337, 469, 673
Bst4CI ACNGT 1 cut(s) 604
BstBAI YACGTR 1 cut(s) 76
BstC8I GCNNGC 3 cut(s) 152, 223, 516
BstDEI CTNAG 2 cut(s) 11, 479
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 3 cut(s) 25, 122, 421
BstKTI GATC 3 cut(s) 340, 472, 676
BstMAI GTCTC 3 cut(s) 166, 369, 482
BstMBI GATC 3 cut(s) 337, 469, 673
BstMCI CGRYCG 1 cut(s) 642
BstMWI GCNNNNNNNGC 2 cut(s) 425, 578
BstSCI CCNGG 1 cut(s) 690
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 5 cut(s) 27, 101, 357, 398, 439
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
BstZI CGGCCG 1 cut(s) 639
Bsu15I ATCGAT 1 cut(s) 713
BsuRI GGCC 3 cut(s) 5, 381, 641
BsuTUI ATCGAT 1 cut(s) 713
BtgZI GCGATG 1 cut(s) 477
Cac8I GCNNGC 3 cut(s) 152, 223, 516
CfoI GCGC 3 cut(s) 25, 122, 421
Cfr10I RCCGGY 2 cut(s) 221, 360
Cfr13I GGNCC 3 cut(s) 380, 688, 694
ClaI ATCGAT 1 cut(s) 713
CseI GACGC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 605
CviAII CATG 1 cut(s) 212
CviQI GTAC 1 cut(s) 605
DdeI CTNAG 2 cut(s) 11, 479
DpnI GATC 3 cut(s) 339, 471, 675
DpnII GATC 3 cut(s) 337, 469, 673
EaeI YGGCCR 2 cut(s) 3, 639
EagI CGGCCG 1 cut(s) 639
EciI GGCGGA 1 cut(s) 240
Ecl136II GAGCTC 1 cut(s) 536
EclXI CGGCCG 1 cut(s) 639
Eco24I GRGCYC 1 cut(s) 538
Eco31I GGTCTC 2 cut(s) 166, 369
Eco47I GGWCC 2 cut(s) 688, 694
Eco52I CGGCCG 1 cut(s) 639
Eco53kI GAGCTC 1 cut(s) 536
Eco57I CTGAAG 3 cut(s) 28, 414, 719
Eco88I CYCGRG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 536
EcoO109I RGGNCCY 1 cut(s) 380
EcoT38I GRGCYC 1 cut(s) 538
FaeI CATG 1 cut(s) 215
FaiI YATR 6 cut(s) 213, 240, 276, 290, 401, 426
FaqI GGGAC 3 cut(s) 44, 188, 376
FatI CATG 1 cut(s) 211
FauI CCCGC 3 cut(s) 102, 135, 391
FbaI TGATCA 2 cut(s) 337, 469
Fnu4HI GCNGC 7 cut(s) 16, 87, 90, 371, 387, 453, 639
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 7 cut(s) 16, 87, 90, 371, 387, 453, 639
FspAI RTGCGCAY 1 cut(s) 121
FspBI CTAG 1 cut(s) 620
FspI TGCGCA 1 cut(s) 121
GlaI GCGC 3 cut(s) 24, 121, 420
GluI GCNGC 7 cut(s) 16, 87, 90, 371, 387, 453, 639
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 3 cut(s) 5, 381, 641
HapII CCGG 4 cut(s) 222, 361, 652, 692
HgaI GACGC 1 cut(s) 378
HhaI GCGC 3 cut(s) 25, 122, 421
Hin1II CATG 1 cut(s) 215
Hin6I GCGC 3 cut(s) 23, 120, 419
HinP1I GCGC 3 cut(s) 23, 120, 419
HinfI GANTC 4 cut(s) 228, 500, 644, 710
HpaII CCGG 4 cut(s) 222, 361, 652, 692
HphI GGTGA 4 cut(s) 124, 200, 286, 347
Hpy188I TCNGA 3 cut(s) 433, 613, 688
Hpy188III TCNNGA 3 cut(s) 266, 329, 677
HpyAV CCTTC 7 cut(s) 52, 326, 404, 442, 547, 618, 625
HpyCH4III ACNGT 1 cut(s) 604
HpyCH4IV ACGT 2 cut(s) 27, 75
HpyCH4V TGCA 1 cut(s) 514
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 578
HpyF3I CTNAG 2 cut(s) 11, 479
HpySE526I ACGT 2 cut(s) 27, 75
Hsp92II CATG 1 cut(s) 215
HspAI GCGC 3 cut(s) 23, 120, 419
KroI GCCGGC 1 cut(s) 221
KroNI GCCGGC 1 cut(s) 223
Ksp22I TGATCA 2 cut(s) 337, 469
Kzo9I GATC 3 cut(s) 337, 469, 673
LmnI GCTCC 3 cut(s) 53, 201, 541
Lsp1109I GCAGC 5 cut(s) 27, 101, 357, 398, 439
LweI GCATC 1 cut(s) 101
MaeI CTAG 1 cut(s) 620
MaeII ACGT 2 cut(s) 27, 75
MaeIII GTNAC 2 cut(s) 102, 174
MalI GATC 3 cut(s) 339, 471, 675
MboI GATC 3 cut(s) 337, 469, 673
MboII GAAGA 1 cut(s) 262
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 1 cut(s) 538
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 136, 299
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 564, 687
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 221
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 564, 576
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 222, 361, 652, 692
MspR9I CCNGG 1 cut(s) 692
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 425, 578
NaeI GCCGGC 1 cut(s) 223
NciI CCSGG 1 cut(s) 692
NdeII GATC 3 cut(s) 337, 469, 673
NgoMIV GCCGGC 1 cut(s) 221
NlaIII CATG 1 cut(s) 215
NlaIV GGNNCC 2 cut(s) 382, 690
NmuCI GTSAC 2 cut(s) 102, 174
NsbI TGCGCA 1 cut(s) 121
PaeR7I CTCGAG 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 223
PfeI GAWTC 4 cut(s) 228, 500, 644, 710
PkrI GCNGC 7 cut(s) 17, 88, 91, 372, 388, 454, 640
Ppu21I YACGTR 1 cut(s) 76
PshBI ATTAAT 1 cut(s) 564
Psp124BI GAGCTC 1 cut(s) 538
PspN4I GGNNCC 2 cut(s) 382, 690
PspPI GGNCC 3 cut(s) 380, 688, 694
PsuI RGATCY 1 cut(s) 673
RsaI GTAC 1 cut(s) 606
RsaNI GTAC 1 cut(s) 605
SacI GAGCTC 1 cut(s) 538
SaqAI TTAA 2 cut(s) 564, 576
SatI GCNGC 7 cut(s) 16, 87, 90, 371, 387, 453, 639
Sau3AI GATC 3 cut(s) 337, 469, 673
Sau96I GGNCC 3 cut(s) 380, 688, 694
ScrFI CCNGG 1 cut(s) 692
SduI GDGCHC 1 cut(s) 538
SfaNI GCATC 1 cut(s) 101
Sfr274I CTCGAG 1 cut(s) 266
SinI GGWCC 2 cut(s) 688, 694
SlaI CTCGAG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 2 cut(s) 136, 299
SsiI CCGC 7 cut(s) 21, 87, 109, 142, 225, 384, 638
SspI AATATT 1 cut(s) 406
SspMI CTAG 1 cut(s) 620
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 1 cut(s) 690
TaaI ACNGT 1 cut(s) 604
TaiI ACGT 2 cut(s) 30, 78
TaqI TCGA 9 cut(s) 53, 134, 231, 258, 267, 409, 458, 548, 713
TasI AATT 2 cut(s) 136, 299
TauI GCSGC 2 cut(s) 89, 641
TfiI GAWTC 4 cut(s) 228, 500, 644, 710
Tru1I TTAA 2 cut(s) 564, 576
Tru9I TTAA 2 cut(s) 564, 576
TseFI GTSAC 2 cut(s) 102, 174
TseI GCWGC 5 cut(s) 15, 89, 370, 386, 452
Tsp45I GTSAC 2 cut(s) 102, 174
TspDTI ATGAA 1 cut(s) 566
VpaK11BI GGWCC 2 cut(s) 688, 694
VspI ATTAAT 1 cut(s) 564
XcmI CCANNNNNNNNNTGG 1 cut(s) 135
XhoI CTCGAG 1 cut(s) 266
XspI CTAG 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.