Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009330

Physical Location & Seq

Genomic Coordinates Forward (+)

10568677 .. 10573665

Gene Structure 4989 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1143000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1449 bp

ATGTCAACTTGCAACCAGACGCCCACTTTCTGCTTCATAAGCTCCGCCATTCCAACGTTCCTACGGTCGGGAATTTCTTATGGAGCAGGTCTTGAAGCTCATAAGGTTAGTATTCTCAAAGAAATTGCGGCGCAATATTCCATCCATTGCTTCCTCTTAGATGAATCCGCATCCTCTATAGACGATGCTATACGCGAGGTTATGCTAGCTTGGCCTAATACCGGAGGCCGGATTTTGTATATAGTATCCAACAACAAGGAATACATGTTTCCTAAACTAAGCAAATGCGGTTGGCTTATCACGCTTCTCAATGTTGAAGGCAGCACCGCATCCACATGTGAGAGTTCAAGCATGGTGTATATTAACAAACTACGCGAGTTGCCTTTGACTATTTGCCAAATAAATAGAAAGGCATTTGGAAACAGCGTTGTGACAGTTGGTTATATTATGAAGCCGTCTCGTGAAGAGGATTTCGCTAAGAGGGGTGCATTCCCTATGTATCCTACTCAAGATGGGTTGATGTTCGTTCCCATCACATTTGATCTCCCTCTAGCTCCTCAATTACAAGAAGTTGATGTAGTTCTCCATAAAGCAACTGATGAAATTATATCCATTAATTTGGATGGATCTTTACCATCTTCTACCAGCATAACATACAGCAGGGGAATGCAGGAATTGCAAAGATATATGGAACATCACCTAGACCTCTGTGTGATTGACCCACTTAGCTCCATTTATCCTGTAGTAGATCGATTGAAAATTCAACAGATTCTACTTGGGTTGGAGGATCTCAAAACCGCCGGTTGCTGCGCAATTAGGGGGCCTAATTTTCTTAAGGTTGATGATTTTAACCAGTCTGATTTGGTTCAAAGCCTATCTGAAGCCAAGTTAGCTCTTCCAAGTATAGTAAAACCTCAAGTTGCTTGTGGTGTGGCCGATGCTCATAGCATGGCAATAGTTTTTAGAGTTGAAGATTTCAAGGATTTGACTGTTCCTCTTCCAGCAATTATACAGGAATATGTGGATCATTCATCAACGCTCTACAAATTTTACGTGTTGGGTGAGAAAGTTTATCATGCAGTTAAGAGCTCTACTCCAAATGCAGATGGTTTGAAAAAATTATCTAGAAGTACGGAGCTCAAACCTTTAGTCTTTGACAGCTTAAAATCCTTACCCACTGCAAAAGGGAACCTGAGTTCTGGAGATGGTAATAGCTCAAAGGCCACTATTGATCTTGAGTTGGTTACTAGTGCAGCTAATTGGCTTATGAGAAATCTTGAGCTGACCATCTTCGGCTTCGATGTTGTTATTGAGGAAGGCACCGGCGACCATGTCATTGTCGATGTAAATTACCTCCCATCATTCAAGGAAGTTCCCAACGAAGTTGCTATCCCTGCGTTTTGGGGTGCGATTAAGAAGAAGTTTGAATTAAAAGGAAGAAATAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010264 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0019637 GO:0019751 GO:0032958 GO:0033517 GO:0043647 GO:0044237 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046173 GO:0047325 GO:0051765 GO:0051766 GO:0052725 GO:0052726 GO:0071704 GO:0090407 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00562 map00562

Pfam Domains

Protein Families

Ins134_P3_kin

Protein Analysis

483

Amino Acids

53.18

Weight (kDa)

5.69

Isoelectric Point (pI)

36.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ins134_P3_kin	PF05770	274 - 460	3.5e-27	Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009330)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43980
fragaria_vesca	FvH4_1g00201
malus_domestica	MD00G1087100.v1.1 MD15G1143000.v1.1
prunus_persica	Prupe.7G270000_v2.0.a1 Prupe.7G270000_v2.0.a1 Prupe.7G270000_v2.0.a1 Prupe.7G270000_v2.0.a1 Prupe.7G270000_v2.0.a1
pyrus_communis	pycom15g12910
rosa_chinensis	RchiOBHm_Chr2g0084491
rosa_laevigata	RLG00000015622
rosa_multiflora	Rmu_sc0003974.1_g000001 Rmu_sc0009647.1_g000006
rosa_roxburghii	Rroxscaffold_2G00156020
rosa_rugosa	Rorug01G0454400 Rorug01G0454500
rosa_samantha	Rh2AG002500 Rh2BG002100 Rh2CG002600 Rh2DG002600
rosa_wichuraiana	Rw2G000220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	811
Acc36I	ACCTGC	1 cut(s)	77
AccB1I	GGYRCC	1 cut(s)	1319
AccII	CGCG	2 cut(s)	195, 375
AciI	CCGC	6 cut(s)	45, 128, 168, 288, 327, 798
AclI	AACGTT	1 cut(s)	56
AclWI	GGATC	3 cut(s)	634, 795, 1032
AcoI	YGGCCR	1 cut(s)	933
AcsI	RAATTY	3 cut(s)	72, 759, 1046
AcuI	CTGAAG	1 cut(s)	900
AcyI	GRCGYC	1 cut(s)	20
AfaI	GTAC	1 cut(s)	1132
AfiI	CCNNNNNNNGG	3 cut(s)	67, 221, 228
AflII	CTTAAG	1 cut(s)	833
AflIII	ACRYGT	3 cut(s)	264, 335, 1053
AhlI	ACTAGT	1 cut(s)	1247
AloI	GAACNNNNNNTCC	2 cut(s)	1180, 1212
Alw21I	GWGCWC	2 cut(s)	1091, 1140
Alw26I	GTCTC	1 cut(s)	462
AlwI	GGATC	3 cut(s)	634, 795, 1032
AoxI	GGCC	5 cut(s)	212, 226, 821, 933, 1221
ApeKI	GCWGC	3 cut(s)	321, 807, 1253
ApoI	RAATTY	3 cut(s)	72, 759, 1046
ArsI	GACNNNNNNTTYG	2 cut(s)	1134, 1166
AseI	ATTAAT	1 cut(s)	615
AspLEI	GCGC	2 cut(s)	133, 812
AspS9I	GGNCC	1 cut(s)	821
AsuHPI	GGTGA	2 cut(s)	689, 1073
AsuNHI	GCTAGC	1 cut(s)	205
BanI	GGYRCC	1 cut(s)	1319
BanII	GRGCYC	2 cut(s)	1091, 1140
BauI	CACGAG	1 cut(s)	459
Bbv12I	GWGCWC	2 cut(s)	1091, 1140
BbvI	GCAGC	3 cut(s)	333, 794, 1265
BccI	CCATC	9 cut(s)	149, 506, 539, 617, 643, 1100, 1199, 1295, 1366
BceAI	ACGGC	1 cut(s)	439
BciVI	GTATCC	2 cut(s)	256, 510
BcoDI	GTCTC	1 cut(s)	462
BcuI	ACTAGT	1 cut(s)	1247
BfaI	CTAG	5 cut(s)	206, 551, 701, 1125, 1248
BfmI	CTRYAG	2 cut(s)	177, 741
BfrI	CTTAAG	1 cut(s)	833
BfuAI	ACCTGC	1 cut(s)	77
BfuI	GTATCC	2 cut(s)	256, 510
BisI	GCNGC	4 cut(s)	129, 322, 808, 1254
BlsI	GCNGC	4 cut(s)	130, 323, 809, 1255
BmgT120I	GGNCC	1 cut(s)	821
BmiI	GGNNCC	3 cut(s)	822, 1190, 1321
BmsI	GCATC	4 cut(s)	175, 179, 338, 928
BmtI	GCTAGC	1 cut(s)	209
BplI	GAGNNNNNCTC	2 cut(s)	1078, 1110
BpmI	CTGGAG	1 cut(s)	1221
BpuEI	CTTGAG	4 cut(s)	492, 900, 1256, 1298
Bsa29I	ATCGAT	1 cut(s)	751
BsaAI	YACGTR	1 cut(s)	1054
BsaHI	GRCGYC	1 cut(s)	20
BsaWI	WCCGGW	1 cut(s)	221
Bsc4I	CCNNNNNNNGG	3 cut(s)	67, 221, 228
Bse118I	RCCGGY	2 cut(s)	800, 1322
Bse1I	ACTGG	1 cut(s)	853
Bse3DI	GCAATG	1 cut(s)	145
BseCI	ATCGAT	1 cut(s)	751
BseGI	GGATG	4 cut(s)	141, 170, 329, 628
BseLI	CCNNNNNNNGG	3 cut(s)	67, 221, 228
BseMI	GCAATG	1 cut(s)	145
BseMII	CTCAG	1 cut(s)	1184
BseNI	ACTGG	1 cut(s)	853
BseRI	GAGGAG	1 cut(s)	546
BseXI	GCAGC	3 cut(s)	333, 794, 1265
BsgI	GTGCAG	1 cut(s)	1272
Bsh1236I	CGCG	2 cut(s)	195, 375
Bsh1285I	CGRYCG	1 cut(s)	68
BshFI	GGCC	5 cut(s)	214, 228, 823, 935, 1223
BshNI	GGYRCC	1 cut(s)	1319
BshVI	ATCGAT	1 cut(s)	751
BsiEI	CGRYCG	1 cut(s)	68
BsiHKAI	GWGCWC	2 cut(s)	1091, 1140
BsiSI	CCGG	4 cut(s)	222, 229, 801, 1323
BslI	CCNNNNNNNGG	3 cut(s)	67, 221, 228
BsmAI	GTCTC	1 cut(s)	462
BsmBI	CGTCTC	1 cut(s)	462
BsmI	GAATGC	2 cut(s)	488, 672
BsnI	GGCC	5 cut(s)	214, 228, 823, 935, 1223
Bsp1286I	GDGCHC	2 cut(s)	1091, 1140
Bsp143I	GATC	6 cut(s)	541, 626, 748, 787, 1024, 1231
BspACI	CCGC	6 cut(s)	45, 128, 168, 288, 327, 798
BspANI	GGCC	5 cut(s)	214, 228, 823, 935, 1223
BspCNI	CTCAG	1 cut(s)	1185
BspDI	ATCGAT	1 cut(s)	751
BspFNI	CGCG	2 cut(s)	195, 375
BspLI	GGNNCC	3 cut(s)	822, 1190, 1321
BspMI	ACCTGC	1 cut(s)	77
BspOI	GCTAGC	1 cut(s)	209
BspPI	GGATC	3 cut(s)	634, 795, 1032
BspQI	GCTCTTC	1 cut(s)	900
BspT107I	GGYRCC	1 cut(s)	1319
BspTI	CTTAAG	1 cut(s)	833
BsrDI	GCAATG	1 cut(s)	145
BsrFI	RCCGGY	2 cut(s)	800, 1322
BsrI	ACTGG	1 cut(s)	853
BssAI	RCCGGY	2 cut(s)	800, 1322
BssMI	GATC	6 cut(s)	541, 626, 748, 787, 1024, 1231
BssNI	GRCGYC	1 cut(s)	20
BssSI	CACGAG	1 cut(s)	459
Bst2BI	CACGAG	1 cut(s)	459
Bst4CI	ACNGT	3 cut(s)	66, 436, 991
Bst6I	CTCTTC	3 cut(s)	459, 900, 1002
BstACI	GRCGYC	1 cut(s)	20
BstAFI	CTTAAG	1 cut(s)	833
BstAPI	GCANNNNNTGC	1 cut(s)	676
BstBAI	YACGTR	1 cut(s)	1054
BstC8I	GCNNGC	1 cut(s)	207
BstDEI	CTNAG	5 cut(s)	157, 278, 477, 725, 1193
BstF5I	GGATG	4 cut(s)	141, 170, 329, 628
BstFNI	CGCG	2 cut(s)	195, 375
BstHHI	GCGC	2 cut(s)	133, 812
BstKTI	GATC	6 cut(s)	544, 629, 751, 790, 1027, 1234
BstMAI	GTCTC	1 cut(s)	462
BstMBI	GATC	6 cut(s)	541, 626, 748, 787, 1024, 1231
BstMCI	CGRYCG	1 cut(s)	68
BstMWI	GCNNNNNNNGC	6 cut(s)	39, 211, 301, 676, 890, 1394
BstNSI	RCATGY	2 cut(s)	268, 339
BstSFI	CTRYAG	2 cut(s)	177, 741
BstUI	CGCG	2 cut(s)	195, 375
BstV1I	GCAGC	3 cut(s)	333, 794, 1265
BstX2I	RGATCY	2 cut(s)	626, 787
BstXI	CCANNNNNNTGG	1 cut(s)	619
BstYI	RGATCY	2 cut(s)	626, 787
Bsu15I	ATCGAT	1 cut(s)	751
BsuI	GTATCC	2 cut(s)	256, 510
BsuRI	GGCC	5 cut(s)	214, 228, 823, 935, 1223
BsuTUI	ATCGAT	1 cut(s)	751
BtsCI	GGATG	4 cut(s)	141, 170, 329, 628
BtsI	GCAGTG	1 cut(s)	1176
BtsIMutI	CAGTG	1 cut(s)	1176
BveI	ACCTGC	1 cut(s)	77
Cac8I	GCNNGC	1 cut(s)	207
CfoI	GCGC	2 cut(s)	133, 812
Cfr10I	RCCGGY	2 cut(s)	800, 1322
Cfr13I	GGNCC	1 cut(s)	821
ClaI	ATCGAT	1 cut(s)	751
CseI	GACGC	1 cut(s)	28
Csp6I	GTAC	1 cut(s)	1131
CviAII	CATG	6 cut(s)	265, 336, 352, 949, 1076, 1331
CviQI	GTAC	1 cut(s)	1131
DdeI	CTNAG	5 cut(s)	157, 278, 477, 725, 1193
DpnI	GATC	6 cut(s)	543, 628, 750, 789, 1026, 1233
DpnII	GATC	6 cut(s)	541, 626, 748, 787, 1024, 1231
EaeI	YGGCCR	1 cut(s)	933
Eam1104I	CTCTTC	3 cut(s)	459, 900, 1002
EarI	CTCTTC	3 cut(s)	459, 900, 1002
EciI	GGCGGA	1 cut(s)	34
Ecl136II	GAGCTC	2 cut(s)	1089, 1138
Eco24I	GRGCYC	2 cut(s)	1091, 1140
Eco53kI	GAGCTC	2 cut(s)	1089, 1138
Eco57I	CTGAAG	1 cut(s)	900
EcoICRI	GAGCTC	2 cut(s)	1089, 1138
EcoO109I	RGGNCCY	1 cut(s)	821
EcoT38I	GRGCYC	2 cut(s)	1091, 1140
Esp3I	CGTCTC	1 cut(s)	462
FaeI	CATG	6 cut(s)	268, 339, 355, 952, 1079, 1334
FatI	CATG	6 cut(s)	264, 335, 351, 948, 1075, 1330
Fnu4HI	GCNGC	4 cut(s)	129, 322, 808, 1254
FokI	GGATG	4 cut(s)	128, 157, 316, 635
FriOI	GRGCYC	2 cut(s)	1091, 1140
Fsp4HI	GCNGC	4 cut(s)	129, 322, 808, 1254
FspBI	CTAG	5 cut(s)	206, 551, 701, 1125, 1248
FspI	TGCGCA	1 cut(s)	811
GlaI	GCGC	2 cut(s)	132, 811
GluI	GCNGC	4 cut(s)	129, 322, 808, 1254
GsuI	CTGGAG	1 cut(s)	1221
HaeIII	GGCC	5 cut(s)	214, 228, 823, 935, 1223
HapII	CCGG	4 cut(s)	222, 229, 801, 1323
HgaI	GACGC	1 cut(s)	28
HhaI	GCGC	2 cut(s)	133, 812
Hin1I	GRCGYC	1 cut(s)	20
Hin1II	CATG	6 cut(s)	268, 339, 355, 952, 1079, 1334
Hin6I	GCGC	2 cut(s)	131, 810
HinP1I	GCGC	2 cut(s)	131, 810
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HinfI	GANTC	2 cut(s)	164, 769
HpaII	CCGG	4 cut(s)	222, 229, 801, 1323
HphI	GGTGA	2 cut(s)	689, 1073
Hpy166II	GTNNAC	1 cut(s)	6
Hpy188I	TCNGA	2 cut(s)	859, 880
Hpy188III	TCNNGA	8 cut(s)	69, 92, 461, 509, 1125, 1200, 1235, 1277
Hpy8I	GTNNAC	1 cut(s)	6
HpyAV	CCTTC	2 cut(s)	311, 1310
HpyCH4III	ACNGT	3 cut(s)	66, 436, 991
HpyCH4IV	ACGT	2 cut(s)	56, 1053
HpyCH4V	TGCA	8 cut(s)	12, 488, 670, 679, 1079, 1103, 1181, 1253
HpyF10VI	GCNNNNNNNGC	6 cut(s)	39, 211, 301, 676, 890, 1394
HpyF3I	CTNAG	5 cut(s)	157, 278, 477, 725, 1193
HpySE526I	ACGT	2 cut(s)	56, 1053
Hsp92I	GRCGYC	1 cut(s)	20
Hsp92II	CATG	6 cut(s)	268, 339, 355, 952, 1079, 1334
HspAI	GCGC	2 cut(s)	131, 810
Kzo9I	GATC	6 cut(s)	541, 626, 748, 787, 1024, 1231
LguI	GCTCTTC	1 cut(s)	900
LmnI	GCTCC	5 cut(s)	47, 83, 559, 734, 1135
Lsp1109I	GCAGC	3 cut(s)	333, 794, 1265
LweI	GCATC	4 cut(s)	175, 179, 338, 928
MaeI	CTAG	5 cut(s)	206, 551, 701, 1125, 1248
MaeII	ACGT	2 cut(s)	56, 1053
MaeIII	GTNAC	2 cut(s)	430, 1243
MalI	GATC	6 cut(s)	543, 628, 750, 789, 1026, 1233
MboI	GATC	6 cut(s)	541, 626, 748, 787, 1024, 1231
MboII	GAAGA	8 cut(s)	476, 630, 887, 983, 989, 1282, 1429, 1449
MflI	RGATCY	2 cut(s)	626, 787
MhlI	GDGCHC	2 cut(s)	1091, 1140
MmeI	TCCRAC	3 cut(s)	77, 273, 762
MseI	TTAA	8 cut(s)	363, 615, 834, 849, 1083, 1163, 1413, 1430
MslI	CAYNNNNRTG	1 cut(s)	334
MspCI	CTTAAG	1 cut(s)	833
MspI	CCGG	4 cut(s)	222, 229, 801, 1323
Mva1269I	GAATGC	2 cut(s)	488, 672
MvnI	CGCG	2 cut(s)	195, 375
MwoI	GCNNNNNNNGC	6 cut(s)	39, 211, 301, 676, 890, 1394
NdeII	GATC	6 cut(s)	541, 626, 748, 787, 1024, 1231
NheI	GCTAGC	1 cut(s)	205
NlaIII	CATG	6 cut(s)	268, 339, 355, 952, 1079, 1334
NlaIV	GGNNCC	3 cut(s)	822, 1190, 1321
NmuCI	GTSAC	1 cut(s)	430
NsbI	TGCGCA	1 cut(s)	811
NspI	RCATGY	2 cut(s)	268, 339
PciI	ACATGT	2 cut(s)	264, 335
PciSI	GCTCTTC	1 cut(s)	900
PctI	GAATGC	2 cut(s)	488, 672
PfeI	GAWTC	2 cut(s)	164, 769
PflFI	GACNNNGTC	1 cut(s)	1331
PkrI	GCNGC	4 cut(s)	130, 323, 809, 1255
Ppu21I	YACGTR	1 cut(s)	1054
PscI	ACATGT	2 cut(s)	264, 335
PshBI	ATTAAT	1 cut(s)	615
Psp124BI	GAGCTC	2 cut(s)	1091, 1140
Psp1406I	AACGTT	1 cut(s)	56
PspN4I	GGNNCC	3 cut(s)	822, 1190, 1321
PspPI	GGNCC	1 cut(s)	821
PsuI	RGATCY	2 cut(s)	626, 787
PsyI	GACNNNGTC	1 cut(s)	1331
RsaI	GTAC	1 cut(s)	1132
RsaNI	GTAC	1 cut(s)	1131
RseI	CAYNNNNRTG	1 cut(s)	334
SacI	GAGCTC	2 cut(s)	1091, 1140
SapI	GCTCTTC	1 cut(s)	900
SaqAI	TTAA	8 cut(s)	363, 615, 834, 849, 1083, 1163, 1413, 1430
SatI	GCNGC	4 cut(s)	129, 322, 808, 1254
Sau3AI	GATC	6 cut(s)	541, 626, 748, 787, 1024, 1231
Sau96I	GGNCC	1 cut(s)	821
SduI	GDGCHC	2 cut(s)	1091, 1140
SfaNI	GCATC	4 cut(s)	175, 179, 338, 928
SfcI	CTRYAG	2 cut(s)	177, 741
SgrAI	CRCCGGYG	1 cut(s)	1322
SmiMI	CAYNNNNRTG	1 cut(s)	334
SmlI	CTYRAG	5 cut(s)	507, 833, 915, 1235, 1277
SmoI	CTYRAG	5 cut(s)	507, 833, 915, 1235, 1277
SpeI	ACTAGT	1 cut(s)	1247
SsiI	CCGC	6 cut(s)	45, 128, 168, 288, 327, 798
SspI	AATATT	1 cut(s)	137
SspMI	CTAG	5 cut(s)	206, 551, 701, 1125, 1248
SstI	GAGCTC	2 cut(s)	1091, 1140
TaaI	ACNGT	3 cut(s)	66, 436, 991
TaiI	ACGT	2 cut(s)	59, 1056
TaqI	TCGA	3 cut(s)	751, 1299, 1341
TauI	GCSGC	1 cut(s)	131
TfiI	GAWTC	2 cut(s)	164, 769
Tru1I	TTAA	8 cut(s)	363, 615, 834, 849, 1083, 1163, 1413, 1430
Tru9I	TTAA	8 cut(s)	363, 615, 834, 849, 1083, 1163, 1413, 1430
TscAI	CASTG	1 cut(s)	1183
TseFI	GTSAC	1 cut(s)	430
TseI	GCWGC	3 cut(s)	321, 807, 1253
Tsp45I	GTSAC	1 cut(s)	430
TspDTI	ATGAA	5 cut(s)	25, 177, 464, 615, 1020
TspGWI	ACGGA	1 cut(s)	1148
TspRI	CASTG	1 cut(s)	1183
Tth111I	GACNNNGTC	1 cut(s)	1331
Vha464I	CTTAAG	1 cut(s)	833
VspI	ATTAAT	1 cut(s)	615
XapI	RAATTY	3 cut(s)	72, 759, 1046
XbaI	TCTAGA	1 cut(s)	1124
XceI	RCATGY	2 cut(s)	268, 339
XspI	CTAG	5 cut(s)	206, 551, 701, 1125, 1248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.