MD15G1248200.v1.1

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014637
Physical Location & Seq
Forward (+)
20692664 .. 20693835
1172 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1248200.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGAGATTGAGGAAGCCAGCAACTGCGAGGCACTGCCTTTGCTTTCTTTAAATCATGTGTCTTTGTTGTGCAGATCAGTGTGGACTTCTATGAGGTTCTATGAAGAAGTGTTGGGCTTTGTTCTCATCAAACGCCCCTCGTCCTTCAACTTCAATGGAGCCTGGTTGTATAACTATGGAATTGGGATACACTTGATTGAAAGTCCGGCAATGGAAGACTATGAATTGCGACCGATCAATCCCAAGGACAACCACATCTCCTTCCAATGCACTGATGTTGGACTTGTTAAGAGGAGGCTGCAAGATATGGGGATGAGGTACGTGACAGCCGTGGTGGAGGATGATGGGGTCAAGGTCGACCAGGTGTTCTTCCATGACCCGGATGGGTATATGATCGAGCTCTGCAACTGTGAGAACATCCCCATCATTCCAATCTCTGCTTGTTACTTCAAGCCCCGAGGCAGTAGTTTCAAGAAGGCATCGACTAGTTGTGGATTCATGGAAACTGTGATGATGGAGAGCTTGAGCCTGGACATGATGAACTTCTCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

185

Amino Acids

21.03

Weight (kDa)

4.86

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 17 - 134 1.7e-11 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014637)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12090 FvH4_1g12090
malus_domestica MD02G1110500.v1.1 MD15G1248200.v1.1
prunus_persica Prupe.7G165900_v2.0.a1
pyrus_communis pycom15g21850
rosa_chinensis RchiOBHm_Chr2g0099751
rosa_laevigata RLG00000016910
rosa_multiflora Rmu_sc0034974.1_g000001 Rmu_sc0034975.1_g000001
rosa_roxburghii Rroxscaffold_2G00142800
rosa_rugosa Rorug02G0082700
rosa_samantha Rh2AG132400 Rh2BG135200 Rh2CG136400 Rh2DG136900
rosa_wichuraiana Rw2G010240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 357
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 379
AgsI TTSAA 5 cut(s) 148, 154, 200, 451, 472
AhlI ACTAGT 1 cut(s) 485
AjnI CCWGG 3 cut(s) 161, 360, 528
AluBI AGCT 2 cut(s) 400, 522
AluI AGCT 2 cut(s) 400, 522
Alw21I GWGCWC 1 cut(s) 402
AlwNI CAGNNNCTG 1 cut(s) 24
Ama87I CYCGRG 1 cut(s) 456
ApeKI GCWGC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 380
AvaI CYCGRG 1 cut(s) 456
BanII GRGCYC 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 285
BccI CCATC 4 cut(s) 338, 377, 431, 508
BceAI ACGGC 1 cut(s) 314
BciT130I CCWGG 3 cut(s) 163, 362, 530
BciVI GTATCC 1 cut(s) 180
BcnI CCSGG 1 cut(s) 380
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 1 cut(s) 486
BfuI GTATCC 1 cut(s) 180
BisI GCNGC 1 cut(s) 299
BlsI GCNGC 1 cut(s) 300
Bme1390I CCNGG 4 cut(s) 163, 362, 380, 530
BmeT110I CYCGRG 1 cut(s) 456
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 4 cut(s) 163, 362, 380, 530
BmsI GCATC 1 cut(s) 488
BpiI GAAGAC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 544
BpuMI CCSGG 1 cut(s) 380
BsaAI YACGTR 1 cut(s) 322
BsaJI CCNNGG 3 cut(s) 243, 330, 457
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 3 cut(s) 163, 362, 530
BseDI CCNNGG 3 cut(s) 243, 330, 457
BseGI GGATG 4 cut(s) 318, 346, 388, 417
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMI GCAATG 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 285
BsgI GTGCAG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 233
BsiEI CGRYCG 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 2 cut(s) 206, 380
BslI CCNNNNNNNGG 1 cut(s) 379
BsoBI CYCGRG 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 3 cut(s) 74, 234, 393
BspLI GGNNCC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 216
BssECI CCNNGG 3 cut(s) 243, 330, 457
BssMI GATC 3 cut(s) 74, 234, 393
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 3 cut(s) 163, 362, 530
Bst4CI ACNGT 2 cut(s) 410, 508
BstBAI YACGTR 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 19
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 4 cut(s) 318, 346, 388, 417
BstKTI GATC 3 cut(s) 77, 237, 396
BstMBI GATC 3 cut(s) 74, 234, 393
BstMCI CGRYCG 1 cut(s) 233
BstNI CCWGG 3 cut(s) 163, 362, 530
BstSCI CCNGG 4 cut(s) 161, 360, 378, 528
BstV1I GCAGC 1 cut(s) 285
BstV2I GAAGAC 1 cut(s) 222
BsuI GTATCC 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 4 cut(s) 318, 346, 388, 417
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 3 cut(s) 32, 84, 270
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 24
CsiI ACCWGGT 1 cut(s) 360
Csp6I GTAC 1 cut(s) 319
CviAII CATG 4 cut(s) 56, 374, 499, 535
CviJI RGCY 9 cut(s) 17, 117, 161, 298, 329, 400, 454, 522, 528
CviKI_1 RGCY 9 cut(s) 17, 117, 161, 298, 329, 400, 454, 522, 528
CviQI GTAC 1 cut(s) 319
DpnI GATC 3 cut(s) 76, 236, 395
DpnII GATC 3 cut(s) 74, 234, 393
DraI TTTAAA 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 243
Eco24I GRGCYC 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 400
EcoRII CCWGG 3 cut(s) 161, 360, 528
EcoT14I CCWWGG 1 cut(s) 243
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 4 cut(s) 59, 377, 502, 538
FatI CATG 4 cut(s) 55, 373, 498, 534
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 299
FokI GGATG 4 cut(s) 325, 353, 395, 404
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 1 cut(s) 299
FspBI CTAG 1 cut(s) 486
GluI GCNGC 1 cut(s) 299
HapII CCGG 2 cut(s) 206, 380
Hin1II CATG 4 cut(s) 59, 377, 502, 538
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 1 cut(s) 495
HpaII CCGG 2 cut(s) 206, 380
Hpy166II GTNNAC 2 cut(s) 84, 358
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 2 cut(s) 84, 358
HpyAV CCTTC 3 cut(s) 154, 271, 469
HpyCH4III ACNGT 2 cut(s) 410, 508
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 4 cut(s) 72, 270, 301, 405
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 4 cut(s) 59, 377, 502, 538
Kzo9I GATC 3 cut(s) 74, 234, 393
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 9 cut(s) 31, 148, 175, 219, 347, 374, 393, 515, 542
Lsp1109I GCAGC 1 cut(s) 285
LweI GCATC 1 cut(s) 488
MabI ACCWGGT 1 cut(s) 360
MaeI CTAG 1 cut(s) 486
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 2 cut(s) 322, 443
MalI GATC 3 cut(s) 76, 236, 395
MboI GATC 3 cut(s) 74, 234, 393
MboII GAAGA 3 cut(s) 116, 227, 361
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 2 cut(s) 180, 224
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 9 cut(s) 4, 22, 87, 148, 285, 288, 309, 331, 452
MseI TTAA 2 cut(s) 50, 288
MspI CCGG 2 cut(s) 206, 380
MspR9I CCNGG 4 cut(s) 163, 362, 380, 530
MvaI CCWGG 3 cut(s) 163, 362, 530
NciI CCSGG 1 cut(s) 380
NdeII GATC 3 cut(s) 74, 234, 393
NlaIII CATG 4 cut(s) 59, 377, 502, 538
NlaIV GGNNCC 1 cut(s) 160
NmuCI GTSAC 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PfeI GAWTC 1 cut(s) 495
PkrI GCNGC 1 cut(s) 300
Ppu21I YACGTR 1 cut(s) 322
Psp124BI GAGCTC 1 cut(s) 402
Psp6I CCWGG 3 cut(s) 161, 360, 528
PspGI CCWGG 3 cut(s) 161, 360, 528
PspN4I GGNNCC 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 24
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacI GAGCTC 1 cut(s) 402
SalI GTCGAC 1 cut(s) 356
SaqAI TTAA 2 cut(s) 50, 288
SatI GCNGC 1 cut(s) 299
Sau3AI GATC 3 cut(s) 74, 234, 393
ScrFI CCNGG 4 cut(s) 163, 362, 380, 530
SduI GDGCHC 1 cut(s) 402
SetI ASST 7 cut(s) 98, 320, 324, 357, 366, 402, 524
SexAI ACCWGGT 1 cut(s) 360
SfaNI GCATC 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
SpeI ACTAGT 1 cut(s) 485
Sse9I AATT 2 cut(s) 180, 224
SspMI CTAG 1 cut(s) 486
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 4 cut(s) 161, 360, 378, 528
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 410, 508
TaiI ACGT 1 cut(s) 324
TaqI TCGA 3 cut(s) 357, 396, 482
TaqII GACCGA 1 cut(s) 247
TasI AATT 2 cut(s) 180, 224
TfiI GAWTC 1 cut(s) 495
Tru1I TTAA 2 cut(s) 50, 288
Tru9I TTAA 2 cut(s) 50, 288
TscAI CASTG 3 cut(s) 39, 84, 277
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 1 cut(s) 298
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 4 cut(s) 117, 237, 487, 554
TspRI CASTG 3 cut(s) 39, 84, 277
XcmI CCANNNNNNNNNTGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.