pycom15g21850

lactoylglutathione

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014637
Physical Location & Seq
Forward (+)
15898548 .. 15899382
835 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g21850.1

Sequence Viewer

Length: 555 bp
ATGGAGATTGAGGAAGCCAGCAACTGCGAGGCACTGCCTTTGCTCTCTTTAAATCATGTGTCTTTGTTGTGCAGATCAGTGTGGACTTCTATGAGGTTCTATGAAGAAGTGTTGGGCTTTGTTCTCATCAAACGCCCCTCGTCTTTCAACTTCAATGGAGCCTGGTTGTATAACTATGGCATTGGGATACACTTGATTGAAAGTCCGGCAATGGAAGACTATGAATTGCGATCGATCAATCCCAAGGACAACCACATCTCCTTCCAATGCACTGATGTTGGACTTGTTAAGAGGAGGCTGCAAGATATGGGGATGAGGTACGTGACGGCCGTGGTGGAGGACGATGGGGTCAAGGTCGACCAGGTGTTCTTCCATGACCCGGATGGGTATATGATCGAGCTCTGCAACTGTGAGAACATCCCCATCATTCCAATCTCTGCTTGTTACTTAAAGCCCCGACGTAGCAGTTTCAAGAAGGCACCGACTAGTTGTGGATTCATGGAAACTGTGATGATGGAGAGCTTGAGCCTGGACATGATGAACTTCTCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

185

Amino Acids

21.09

Weight (kDa)

4.97

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 17 - 134 3.8e-11 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014637)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12090 FvH4_1g12090
malus_domestica MD02G1110500.v1.1 MD15G1248200.v1.1
prunus_persica Prupe.7G165900_v2.0.a1
pyrus_communis pycom15g21850
rosa_chinensis RchiOBHm_Chr2g0099751
rosa_laevigata RLG00000016910
rosa_multiflora Rmu_sc0034974.1_g000001 Rmu_sc0034975.1_g000001
rosa_roxburghii Rroxscaffold_2G00142800
rosa_rugosa Rorug02G0082700
rosa_samantha Rh2AG132400 Rh2BG135200 Rh2CG136400 Rh2DG136900
rosa_wichuraiana Rw2G010240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 478
AccI GTMKAC 1 cut(s) 357
AcoI YGGCCR 1 cut(s) 327
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 379
AgsI TTSAA 4 cut(s) 148, 154, 200, 472
AhlI ACTAGT 1 cut(s) 485
AjnI CCWGG 3 cut(s) 161, 360, 528
AluBI AGCT 2 cut(s) 400, 522
AluI AGCT 2 cut(s) 400, 522
Alw21I GWGCWC 1 cut(s) 402
AlwNI CAGNNNCTG 1 cut(s) 24
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 380
BanI GGYRCC 1 cut(s) 478
BanII GRGCYC 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 285
BccI CCATC 4 cut(s) 338, 377, 431, 508
BceAI ACGGC 2 cut(s) 314, 342
BciT130I CCWGG 3 cut(s) 163, 362, 530
BciVI GTATCC 1 cut(s) 180
BcnI CCSGG 1 cut(s) 380
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 1 cut(s) 486
BfuI GTATCC 1 cut(s) 180
BisI GCNGC 1 cut(s) 299
BlsI GCNGC 1 cut(s) 300
Bme1390I CCNGG 4 cut(s) 163, 362, 380, 530
BmiI GGNNCC 2 cut(s) 160, 480
BmrFI CCNGG 4 cut(s) 163, 362, 380, 530
BpiI GAAGAC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 544
BpuMI CCSGG 1 cut(s) 380
Bsa29I ATCGAT 1 cut(s) 233
BsaAI YACGTR 1 cut(s) 322
BsaJI CCNNGG 2 cut(s) 243, 330
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 3 cut(s) 163, 362, 530
BseCI ATCGAT 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 243, 330
BseGI GGATG 3 cut(s) 318, 388, 417
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMI GCAATG 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 307
BseX3I CGGCCG 1 cut(s) 327
BseXI GCAGC 1 cut(s) 285
BsgI GTGCAG 1 cut(s) 91
Bsh1285I CGRYCG 2 cut(s) 233, 330
BshFI GGCC 1 cut(s) 329
BshNI GGYRCC 1 cut(s) 478
BshVI ATCGAT 1 cut(s) 233
BsiEI CGRYCG 2 cut(s) 233, 330
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 2 cut(s) 206, 380
BslI CCNNNNNNNGG 1 cut(s) 379
BsnI GGCC 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 4 cut(s) 74, 230, 234, 393
BspANI GGCC 1 cut(s) 329
BspDI ATCGAT 1 cut(s) 233
BspLI GGNNCC 2 cut(s) 160, 480
BspT107I GGYRCC 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 216
BssECI CCNNGG 2 cut(s) 243, 330
BssMI GATC 4 cut(s) 74, 230, 234, 393
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 3 cut(s) 163, 362, 530
Bst4CI ACNGT 2 cut(s) 410, 508
BstBAI YACGTR 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 19
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 3 cut(s) 318, 388, 417
BstKTI GATC 4 cut(s) 77, 233, 237, 396
BstMBI GATC 4 cut(s) 74, 230, 234, 393
BstMCI CGRYCG 2 cut(s) 233, 330
BstNI CCWGG 3 cut(s) 163, 362, 530
BstSCI CCNGG 4 cut(s) 161, 360, 378, 528
BstV1I GCAGC 1 cut(s) 285
BstV2I GAAGAC 1 cut(s) 222
BstZI CGGCCG 1 cut(s) 327
Bsu15I ATCGAT 1 cut(s) 233
BsuI GTATCC 1 cut(s) 180
BsuRI GGCC 1 cut(s) 329
BsuTUI ATCGAT 1 cut(s) 233
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 3 cut(s) 318, 388, 417
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 3 cut(s) 32, 84, 270
Cac8I GCNNGC 1 cut(s) 19
CaiI CAGNNNCTG 1 cut(s) 24
ClaI ATCGAT 1 cut(s) 233
CsiI ACCWGGT 1 cut(s) 360
Csp6I GTAC 1 cut(s) 319
CviAII CATG 4 cut(s) 56, 374, 499, 535
CviJI RGCY 9 cut(s) 17, 117, 161, 298, 329, 400, 454, 522, 528
CviKI_1 RGCY 9 cut(s) 17, 117, 161, 298, 329, 400, 454, 522, 528
CviQI GTAC 1 cut(s) 319
DpnI GATC 4 cut(s) 76, 232, 236, 395
DpnII GATC 4 cut(s) 74, 230, 234, 393
DraI TTTAAA 1 cut(s) 51
DrdI GACNNNNNNGTC 1 cut(s) 347
DseDI GACNNNNNNGTC 1 cut(s) 347
EaeI YGGCCR 1 cut(s) 327
EagI CGGCCG 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 400
EclXI CGGCCG 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 243
Eco24I GRGCYC 1 cut(s) 402
Eco52I CGGCCG 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 400
EcoICRI GAGCTC 1 cut(s) 400
EcoRII CCWGG 3 cut(s) 161, 360, 528
EcoT14I CCWWGG 1 cut(s) 243
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 4 cut(s) 59, 377, 502, 538
FatI CATG 4 cut(s) 55, 373, 498, 534
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 299
FokI GGATG 3 cut(s) 325, 395, 404
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 1 cut(s) 299
FspBI CTAG 1 cut(s) 486
GluI GCNGC 1 cut(s) 299
HaeIII GGCC 1 cut(s) 329
HapII CCGG 2 cut(s) 206, 380
Hin1II CATG 4 cut(s) 59, 377, 502, 538
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 1 cut(s) 495
HpaII CCGG 2 cut(s) 206, 380
Hpy166II GTNNAC 2 cut(s) 84, 358
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 2 cut(s) 84, 358
Hpy99I CGWCG 1 cut(s) 462
HpyAV CCTTC 2 cut(s) 271, 469
HpyCH4III ACNGT 2 cut(s) 410, 508
HpyCH4IV ACGT 2 cut(s) 321, 460
HpyCH4V TGCA 4 cut(s) 72, 270, 301, 405
HpySE526I ACGT 2 cut(s) 321, 460
Hsp92II CATG 4 cut(s) 59, 377, 502, 538
Kzo9I GATC 4 cut(s) 74, 230, 234, 393
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 9 cut(s) 31, 148, 175, 219, 347, 374, 393, 515, 542
Lsp1109I GCAGC 1 cut(s) 285
MabI ACCWGGT 1 cut(s) 360
MaeI CTAG 1 cut(s) 486
MaeII ACGT 2 cut(s) 321, 460
MaeIII GTNAC 2 cut(s) 322, 443
MalI GATC 4 cut(s) 76, 232, 236, 395
MboI GATC 4 cut(s) 74, 230, 234, 393
MboII GAAGA 3 cut(s) 116, 227, 361
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 8 cut(s) 4, 22, 87, 148, 285, 288, 309, 331
MseI TTAA 3 cut(s) 50, 288, 449
MspI CCGG 2 cut(s) 206, 380
MspR9I CCNGG 4 cut(s) 163, 362, 380, 530
MvaI CCWGG 3 cut(s) 163, 362, 530
NciI CCSGG 1 cut(s) 380
NdeII GATC 4 cut(s) 74, 230, 234, 393
NlaIII CATG 4 cut(s) 59, 377, 502, 538
NlaIV GGNNCC 2 cut(s) 160, 480
NmuCI GTSAC 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PfeI GAWTC 1 cut(s) 495
PkrI GCNGC 1 cut(s) 300
Ple19I CGATCG 1 cut(s) 233
Ppu21I YACGTR 1 cut(s) 322
Psp124BI GAGCTC 1 cut(s) 402
Psp6I CCWGG 3 cut(s) 161, 360, 528
PspGI CCWGG 3 cut(s) 161, 360, 528
PspN4I GGNNCC 2 cut(s) 160, 480
PstNI CAGNNNCTG 1 cut(s) 24
PvuI CGATCG 1 cut(s) 233
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacI GAGCTC 1 cut(s) 402
SalI GTCGAC 1 cut(s) 356
SaqAI TTAA 3 cut(s) 50, 288, 449
SatI GCNGC 1 cut(s) 299
Sau3AI GATC 4 cut(s) 74, 230, 234, 393
ScrFI CCNGG 4 cut(s) 163, 362, 380, 530
SduI GDGCHC 1 cut(s) 402
SetI ASST 8 cut(s) 98, 320, 324, 357, 366, 402, 463, 524
SexAI ACCWGGT 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
SpeI ACTAGT 1 cut(s) 485
Sse9I AATT 1 cut(s) 224
SspMI CTAG 1 cut(s) 486
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 4 cut(s) 161, 360, 378, 528
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 410, 508
TaiI ACGT 2 cut(s) 324, 463
TaqI TCGA 3 cut(s) 233, 357, 396
TasI AATT 1 cut(s) 224
TfiI GAWTC 1 cut(s) 495
Tru1I TTAA 3 cut(s) 50, 288, 449
Tru9I TTAA 3 cut(s) 50, 288, 449
TscAI CASTG 3 cut(s) 39, 84, 277
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 1 cut(s) 298
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 4 cut(s) 117, 237, 487, 554
TspRI CASTG 3 cut(s) 39, 84, 277
XcmI CCANNNNNNNNNTGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.